A detection device for fluorescent probe production
By using a conduit and an inverted U-shaped tube design, the problem of insufficient mixing between the fluorescent probe and the reaction matrix was solved, resulting in more efficient fluorescent probe detection and improved detection accuracy and stability.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHANDONG UNIV OF TECH
- Filing Date
- 2025-07-22
- Publication Date
- 2026-05-29
AI Technical Summary
The existing fluorescent probes do not react sufficiently with the reaction matrix, which affects the detection effect.
The design employs multiple conduits and an inverted U-shaped tube to ensure that the fluorescent probe is uniformly introduced into the reaction matrix. The fluorescence reaction is excited by ultraviolet light, and the combination of temperature regulation and supplementary lighting improves the sufficiency of the mixing reaction and the accuracy of detection.
It improves the mixing and reaction effect of fluorescent probes and reaction matrix, ensures the accuracy and stability of detection, avoids backflow, and simplifies the operation process.
Smart Images
Figure CN224303566U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of detection technology, and in particular to a detection device for the production of fluorescent probes. Background Technology
[0002] Currently, the main methods for detecting fluorescent probes are single-point assay and charge-coupled device (CCD) fluorescence imaging. Single-point assay involves adding the fluorescent probe to be detected into a specific solution and then manually detecting it under ultraviolet light using a microscope. CCD fluorescence imaging uses a high-definition photoelectric device to monitor the probe and then detects it after image magnification, which facilitates observation of the fluorescent probe detection process. However, high-definition photoelectric devices are expensive and complex to use. Single-point assay cannot detect the early reactions of the fluorescent probe and requires a good detection environment, which is greatly affected by the external environment.
[0003] A search revealed a Chinese patent with publication number CN 214794469 U, which discloses a testing device for producing fluorescent probes. The device includes a box, a light-shielding cover rotatably connected to the top of the box, feet at the bottom of the box, temperature regulators on both sides of the inner cavity of the box, a colorimetric card at the bottom of the box, a slide fixedly connected to both sides of the colorimetric card, a reaction tank connected to the middle of the slide, an ultraviolet lamp on the side of the reaction tank, a capillary tube on the side of the reaction tank, a detection lens fixedly connected to both sides of the slide, the reaction tank being made of a light-transmitting material, a convex cover near the detection lens on the side of the reaction tank, and an air vent on the side of the reaction tank away from the capillary tube.
[0004] The aforementioned device has shortcomings in the mixing and reaction of the fluorescent probe and the reaction matrix. This device directly introduces the fluorescent probe formulation into the reaction tank via a capillary tube to react with the reaction matrix. This design prevents the fluorescent probe formulation from fully mixing with the reaction matrix, resulting in incomplete reaction and consequently affecting the detection effect of the fluorescent probe. Therefore, the device needs to be improved. Utility Model Content
[0005] The purpose of this invention is to provide a detection device for the production of fluorescent probes, so as to solve the problem mentioned in the background art that the insufficient mixing reaction of fluorescent probes and reaction matrix affects the detection effect.
[0006] To solve the above problems, the technical solution adopted by this utility model is as follows:
[0007] A detection device for producing fluorescent probes, comprising:
[0008] A reaction chamber, the reaction chamber including a bottom plate, one end of which extends outward from the reaction chamber, and a compartment opening is provided on one side wall of the reaction chamber;
[0009] A detection lens, which is fixedly installed on the top wall inside the reaction chamber;
[0010] The bottom plate has a square slot on its upper side, and a bracket is slidably connected inside the square slot. A reaction vessel is fixedly mounted on the bracket. The bracket is fixedly connected to a side plate via a first support rod. The size of the side plate is adapted to the size of the chamber opening. The outer wall of the reaction vessel has several mounting holes that communicate with the internal cavity of the reaction vessel. A conduit is installed inside the mounting hole. The conduit has a C-shaped structure design. One end of the conduit is connected to an inverted U-shaped tube. One end of the inverted U-shaped tube is connected to an injection tube. One end of the injection tube passes through the side plate and extends to the outside of the reaction chamber.
[0011] Two ultraviolet lamps are symmetrically arranged on both sides of the reaction vessel and fixedly mounted on the top wall of the support.
[0012] Preferably, an auxiliary handle is fixedly connected to one side wall of the side plate.
[0013] Preferably, a second support rod and a third support rod are fixedly connected to one side wall of the side plate, a fill light is fixedly connected to one end of the second support rod, and a tray is fixedly connected to one end of the third support rod. A colorimetric card is provided on the tray, and the tray is positioned above the fill light.
[0014] Preferably, the two inner side walls of the reaction chamber are symmetrically connected to two temperature regulators.
[0015] Preferably, two fourth support rods are fixedly connected to the two inner side walls of the reaction chamber, and a cover is fixedly connected to one end of each of the two fourth support rods. The cover has a convex structure design.
[0016] Compared with the prior art, this utility model has the following advantages:
[0017] This invention firstly introduces the fluorescent probe evenly into the reaction matrix by setting multiple conduits, increasing the number of mixing reaction points and ensuring that the fluorescent probe and the reaction matrix can be fully mixed and undergo a preliminary reaction, thereby improving the accuracy and reliability of detection and solving the problem of insufficient mixing between the fluorescent probe and the reaction matrix in the prior art. Secondly, the inverted U-shaped tube design used in the device effectively avoids the backflow of the reaction matrix and the fluorescent probe solution, ensuring the smooth progress of the detection process and further improving the stability of detection. Attached Figure Description
[0018] Figure 1 This is a schematic diagram of the overall external structure of a detection device for producing fluorescent probes;
[0019] Figure 2 This is a schematic cross-sectional view of a detection device for producing fluorescent probes.
[0020] Figure 3 This is a first-view structural schematic diagram of a detection device for producing fluorescent probes.
[0021] Figure 4 A detection device for the production of fluorescent probes Figure 1 Enlarged view of a portion of point A in the middle.
[0022] In the diagram: 1. Reaction chamber; 2. Probe lens; 3. Bracket; 4. Reaction dish; 5. Guide tube; 6. Inverted U-shaped tube; 7. Injection tube; 8. First support rod; 9. Side plate; 10. Ultraviolet lamp; 11. Auxiliary handle; 12. Second support rod; 13. Third support rod; 14. Supplemental light; 15. Tray; 16. Temperature regulator; 17. Fourth support rod; 18. Cover; 19. Base plate. Detailed Implementation
[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0024] Example:
[0025] Please see Figures 1-4 As shown, this utility model is a detection device for the production of fluorescent probes, including...
[0026] The reaction chamber 1 includes a bottom plate 19, one end of which extends outward from the reaction chamber 1, and a compartment opening is provided on one side wall of the reaction chamber 1.
[0027] Detection lens 2, which is fixedly installed on the top wall inside the reaction chamber 1;
[0028] The base plate 19 has a square slot on its upper wall. A bracket 3 is slidably connected inside the square slot. A reaction vessel 4 is fixedly mounted on the bracket 3. A side plate 9 is fixedly connected to the bracket 3 via a first support rod 8. The size of the side plate 9 is adapted to the size of the chamber opening. The outer wall of the reaction vessel 4 has several mounting holes that communicate with the internal cavity of the reaction vessel 4. A conduit 5 is installed inside the mounting holes. The conduit 5 has a C-shaped structure design. One end of several conduits 5 is connected to an inverted U-shaped tube 6. One end of the inverted U-shaped tube 6 is connected to an injection tube 7. One end of the injection tube 7 passes through the side plate 9 and extends to the outside of the reaction chamber 1.
[0029] Two ultraviolet lamps 10 are symmetrically arranged on both sides of the reaction vessel 4 and fixedly mounted on the top wall of the bracket 3.
[0030] Both the bracket 3 and the reaction vessel 4 are made of transparent material.
[0031] As can be seen from the above, when using it, the staff first utilizes the characteristic of the bracket 3 slidingly connected inside the square slot, and then easily moves the bracket 3 by pulling the side plate 9. The bracket 3 is then pulled to the outside of the reaction chamber 1. At this time, the staff pours the reaction matrix into the reaction dish 4, and then moves the bracket 3 into the reaction chamber 1. The detection lens 2 is activated, and the fluorescent probe to be detected is pumped into the reaction dish 4 through the injection tube 7. It is fully mixed with the reaction matrix inside the reaction dish 4 to carry out the initial reaction. Then the ultraviolet lamp 10 is turned on. The ultraviolet lamp 10 emits ultraviolet light, which causes the detection matrix to combine with the fluorescent probe and be excited to fluoresce, so as to obtain the detection result.
[0032] During the process of pumping the fluorescent probe into the reaction dish 4, the fluorescent probe first enters the inverted U-shaped tube 6 through the injection tube 7, and then enters the conduit 5 through the inverted U-shaped tube 6. There are several conduits 5. The fluorescent probe preparation is introduced into the reaction matrix through several conduits 5, which increases the mixing reaction points and ensures that the mixing is sufficient. Moreover, the inverted U-shaped tube 6 prevents the backflow of the mixed reaction matrix and the fluorescent probe solution. The structural design is user-friendly and ensures that the detection is carried out normally.
[0033] refer to Figure 3 As shown, an auxiliary handle 11 is fixedly connected to one side wall of the side plate 9.
[0034] Specifically, personnel can move the side plate 9 by pulling the auxiliary handle 11, which in turn moves the bracket 3, making the operation convenient and quick.
[0035] refer to Figure 2 As shown, a second support rod 12 and a third support rod 13 are fixedly connected to one side wall of the side plate 9. A fill light 14 is fixedly connected to one end of the second support rod 12, and a tray 15 is fixedly connected to one end of the third support rod 13. A colorimetric card is provided on the tray 15, and the tray 15 is positioned above the fill light 14. The tray 15 is made of transparent material.
[0036] As can be seen from the above, after the fluorescent probe reacts with the reaction matrix, the supplementary light 14 is turned on, and then the colorimetric card placed therein can be used for colorimetric identification in order to obtain the results.
[0037] refer to Figure 2 As shown, the reaction chamber 1 has two temperature regulators 16 that are symmetrically connected to the two inner side walls.
[0038] As can be seen from the above, by setting the temperature regulator 16, it is easy to control the reaction environment temperature inside the reaction chamber 1 and avoid the influence of the external environment temperature.
[0039] refer to Figure 2 As shown, two fourth support rods 17 are fixedly connected to the two inner side walls of the reaction chamber 1. One end of each of the two fourth support rods 17 is fixedly connected to a cover 18. The cover 18 has a convex structure design and is made of transparent material.
[0040] As can be seen from the above, the cover 18 has a convex structure design, which uses the principle of convex mirror to magnify and observe the reaction phenomenon, and the structural design is user-friendly.
[0041] All standard parts used in this invention can be purchased from the market, and irregularly shaped parts can be customized according to the description and drawings. The specific connection methods for each part all employ conventional methods such as bolts, rivets, and welding, which are mature technologies in the prior art. The machinery, parts, and equipment all use conventional models in the prior art, and the circuit connections also use conventional connection methods in the prior art, which will not be detailed here. Any content not described in detail in this specification belongs to the prior art known to those skilled in the art.
[0042] In the description of this utility model, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. "A plurality of" means two or more, unless otherwise explicitly specified.
[0043] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.
[0044] In this utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.
[0045] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.
[0046] The accompanying drawings of the embodiments disclosed in this utility model only involve the structures involved in the embodiments disclosed in this utility model. Other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of this utility model can be combined with each other.
[0047] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.
Claims
1. A detection device for producing fluorescent probes, characterized in that: include The reaction chamber (1) includes a bottom plate (19), one end of which extends outward from the reaction chamber (1), and a compartment opening is provided on one side wall of the reaction chamber (1). The detection lens (2) is fixedly installed on the top wall inside the reaction chamber (1); The bottom plate (19) has a square slot on its upper wall. A bracket (3) is slidably connected inside the square slot. A reaction vessel (4) is fixedly mounted on the bracket (3). The bracket (3) is fixedly connected to a side plate (9) via a first support rod (8). The size of the side plate (9) is adapted to the size of the chamber opening. The outer wall of the reaction vessel (4) has several mounting holes that communicate with the internal cavity of the reaction vessel (4). A conduit (5) is installed inside the mounting hole. The conduit (5) has a C-shaped structure design. One end of several conduits (5) is connected to an inverted U-shaped tube (6). One end of the inverted U-shaped tube (6) is connected to an injection tube (7). One end of the injection tube (7) passes through the side plate (9) and extends to the outside of the reaction chamber (1). Ultraviolet lamps (10) are arranged symmetrically on both sides of the reaction vessel (4) and fixedly mounted on the top wall of the bracket (3).
2. The detection device for producing fluorescent probes according to claim 1, characterized in that: An auxiliary handle (11) is fixedly connected to one side wall of the side plate (9).
3. The detection device for producing fluorescent probes according to claim 1, characterized in that: The side wall of the side plate (9) is also fixedly connected to a second support rod (12) and a third support rod (13). One end of the second support rod (12) is fixedly connected to a fill light (14), and one end of the third support rod (13) is fixedly connected to a tray (15). A colorimetric card is provided on the tray (15), and the tray (15) is positioned above the fill light (14).
4. The detection device for producing fluorescent probes according to claim 1, characterized in that: The reaction chamber (1) has two temperature regulators (16) that are symmetrically connected to the two inner side walls.
5. The detection device for producing fluorescent probes according to claim 1, characterized in that: The reaction chamber (1) has two fourth support rods (17) fixedly connected to the two inner side walls. One end of each of the two fourth support rods (17) is fixedly connected to a cover (18), which has a convex structure design.