An immunobiochemical reaction device

Abstract

The utility model relates to an immune biochemical reaction device belongs to immune biochemical reaction device technical field, the immune biochemical reaction device, include: base, apron, feeding assembly, motor base, reaction cup, rotor, electric push rod and support, and feeding assembly is located on the support, and is located reaction cup top, base adopts aluminium alloy integrated mould, and motor base realizes rotation through traditional screw rod drive mechanism, two magnetic rings are respectively with the both ends magnetic connection of magnetic rotor, drive the rotation of magnetic rotor of the magnetic interface, and the bottom of magnetic rotor is through the contactless transmission of brushless motor in motor base with magnetic coupling mode, avoids the leakage risk caused by mechanical seal, and the electric push rod is used for adjusting the height of apron, is used for sealing reaction cup, and the support is the portal type structure, through motor base, magnetic stirring, sealed sample adding etc. design, effectively solve the problem of bubble production, sample pollution and uneven mixing of traditional device, be applicable to the immune analysis scene such as chemiluminescence.

Description

Technical Field

[0001] This utility model relates to the field of immunobiochemical reaction device technology, and in particular to an immunobiochemical reaction device. Background Technology

[0002] Immunoassay is based on the immunological reaction of antigen-antibody interaction. It uses enzymes and other labeling agents to label antigens and antibodies, and analyzes the antigens or antibodies to be tested in human samples. It is mainly used in hospital laboratories, independent third-party laboratories, blood testing centers and other institutions to perform quantitative, semi-quantitative or qualitative detection of the content of various analytes in human body fluids, and to diagnose infectious diseases, tumors, endocrine function, cardiovascular diseases, eugenics and autoimmune diseases.

[0003] Immunoassay testing typically uses traditional mechanical paddle stirring or centrifugal mixing methods, which easily introduce air bubbles and generate mechanical shear forces, leading to a decrease in the efficiency of antigen-antibody complex formation. At the same time, magnetic beads or enzyme-labeled reagents in high-viscosity samples (such as whole blood) are easily destroyed by shear forces. Open sample addition processes pose a risk of air pollution, and aerosols or dust particles in the environment can enter the reaction system through pipetting, causing false positive results. These shortcomings directly limit the sensitivity and repeatability of the test, making it difficult to meet the clinical POCT demand for rapid and high-precision testing. Utility Model Content

[0004] Therefore, it is necessary to provide an immunobiochemical reaction device to address the problems that traditional mechanical paddle stirring or centrifugal mixing methods easily introduce air bubbles and generate mechanical shear forces, leading to a decrease in the efficiency of antigen-antibody complex formation, and that magnetic beads or enzyme-labeled reagents in high-viscosity samples (such as whole blood) are easily destroyed by shear forces.

[0005] An immunochemical reaction device includes: a base, a motor seat movably disposed inside the base, a reaction cup movably disposed above the motor seat, and a rotor movably disposed inside the reaction cup and magnetically connected to the motor seat below;

[0006] A cover plate is disposed above the reaction cup, and the reaction cup and the cover plate form a sealed reaction chamber. An electric push rod is disposed above the cover plate, and a bracket is disposed on one side of the electric push rod.

[0007] The feeding assembly is mounted on the support and positioned above the reaction cup.

[0008] In one embodiment, the upper part of the motor base is provided with a groove, and the lower part of the reaction cup is embedded in the motor base through the groove.

[0009] In one embodiment, a limiting groove is formed in the middle of the base, and a controller is electrically connected to one side of the motor base. One side of the controller is embedded inside the base through the limiting groove.

[0010] In one embodiment, a sealing ring is fixedly connected to the lower part of the cover plate, and a sealing groove is formed annularly above the reaction cup. The lower part of the sealing ring is embedded into the interior of the cover plate through the sealing groove.

[0011] In one embodiment, a support plate is movably inserted into the middle of the bracket, and one side of the electric actuator is fixed to the support plate.

[0012] In one embodiment, the feeding assembly includes a sealed container located on the reaction cup, with a connecting pipe fixedly inserted at the lower center of the sealed container. The lower end of the connecting pipe passes through the cover plate and is inserted into the interior of the reaction cup.

[0013] In one embodiment, the connecting pipe has a flexible tube and a one-way valve in the middle, the two ends of the flexible tube are sleeved on the port surface of the connecting pipe, and the one-way valve is located below the flexible tube.

[0014] In one embodiment, the rotor has spiral grooves on its surface and rotates at the bottom center of the reaction cup. Beneficial effects

[0015] 1. The base is made of one-piece aluminum alloy. The motor base rotates through a screw drive mechanism, driving the disc to rotate. Two magnetic rings are symmetrically arranged inside the disc, and the two magnetic rings are magnetically connected to the two ends of the magnetic rotor. When the magnetic rings rotate, they drive the connected magnetic rotor to rotate. The reaction cup is made of transparent quartz glass with a hydrophobic coating on the inner wall to prevent sample from sticking to the wall. The bottom of the magnetic rotor is magnetically coupled to the brushless motor in the motor base for contactless transmission, avoiding the risk of leakage caused by mechanical seals. An electric actuator is embedded in the center of the cover plate, which is used to adjust the height of the cover plate and seal the reaction cup. The support is a gantry structure. Through the design of the motor base, magnetic stirring, and sealed sample addition, it effectively solves the problems of bubble generation, sample contamination and uneven mixing in traditional devices, and is suitable for immunoassay scenarios such as chemiluminescence.

[0016] 2. The rotor surface is machined with double helical grooves, which generate axial vortices during rotation, causing the reaction liquid to circulate vertically, improving mixing efficiency compared to a single helical design. At the same time, the chamfered design of the helical groove edges avoids shear force concentration and protects the integrity of magnetic bead reagents. Magnets are embedded at both ends of the magnetic rotor and form multi-pole magnetic coupling with the annular magnetic array in the motor base, which improves efficiency compared to traditional gear transmission. This layout allows the reaction cup to complete the entire process from sample addition to detection in a sealed state. The sealing pressure can be dynamically adjusted by an electric actuator to adapt to the packaging requirements of samples of different volumes, effectively solving the cross-contamination problem caused by open operation in traditional devices. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a schematic diagram of the reaction cup structure of this utility model;

[0020] Figure 3 This is a schematic diagram of the support plate structure of this utility model;

[0021] Figure 4 This is a schematic diagram of the rotor structure of this utility model;

[0022] Figure 5 This is a schematic diagram of the feeding component structure of this utility model.

[0023] Figure label:

[0024] 100. Base; 101. Limiting groove; 200. Motor base; 201. Reaction cup; 202. Sealing groove; 203. Cover plate; 204. Sealing ring; 205. Controller; 300. Bracket; 400. Support plate; 401. Electric actuator; 500. Feeding assembly; 501. Sealed tank; 502. One-way valve; 503. Connecting pipe; 504. Flexible pipe; 600. Rotor; 601. Spiral groove. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0026] The following is combined with Figures 1-5 This invention describes the immunobiochemical reaction device.

[0027] In one embodiment, an immunobiochemical reaction device includes: a base 100, a cover plate 203, and a feeding assembly 500. A motor base 200 is movably disposed inside the base 100, and a reaction cup 201 is movably disposed above the motor base 200. A rotor 600 magnetically connected to the lower motor base 200 is movably disposed inside the reaction cup 201. The cover plate 203 is disposed above the reaction cup 201, and the reaction cup 201 and the cover plate 203 form a sealed reaction chamber. An electric push rod 401 is disposed above the cover plate 203, and a bracket 300 is disposed on one side of the electric push rod 401. The feeding assembly 500 is disposed on the bracket 300 and located above the reaction cup 201.

[0028] In this embodiment, the base 100 is integrally formed from aluminum alloy, and the motor base 200 rotates through a traditional lead screw transmission mechanism, driving the disc to rotate. Two magnetic rings are symmetrically arranged inside the disc, and the two magnetic rings are magnetically connected to the two ends of the magnetic rotor 600 respectively. When the magnetic rings rotate, they will drive the connected magnetic rotor 600 to rotate. It should be noted that the motor base 200 is a relatively mature product on the market. The appropriate model can be selected according to the actual use requirements. The reaction cup 201 is made of transparent quartz glass, and the inner wall is coated with a hydrophobic coating to prevent the sample from sticking to the wall. The bottom of the magnetic rotor 600 is connected to the brushless motor in the motor base 200 through magnetic coupling without contact, avoiding the risk of leakage caused by mechanical seals.

[0029] An electric actuator 401 is embedded in the center of the cover plate 203. The electric actuator 401 is used to adjust the height of the cover plate 203 and to seal the reaction cup 201. The support 300 has a gantry structure. Through the design of motor base 200, magnetic stirring, and sealed sample addition, it effectively solves the problems of bubble generation, sample contamination and uneven mixing in traditional devices. It is suitable for immunoassay scenarios such as chemiluminescence.

[0030] like Figure 1 , Figure 2 and Figure 3As shown, a groove is provided on the upper part of the motor base 200, and the reaction cup 201 is embedded in the motor base 200 through the groove. A limiting groove 101 is provided in the middle of the base 100. A controller 205 is electrically connected to one side of the motor base 200, and one side of the controller 205 is embedded in the base 100 through the limiting groove 101.

[0031] In this embodiment, the limiting groove 101 in the middle of the base 100 is used to support and limit the motor base 200. The reaction cup 201 maintains stability during magnetic stirring, effectively avoiding vibration noise caused by eccentricity. The controller 205 is connected to the brushless motor in the motor base 200 through a flexible FPC cable. The cable length is reserved for extension and retraction to avoid wire breakage caused by rotation and twisting, so that the motor base 200 maintains stable communication with the controller 205. At the same time, the magnetic coupling effect of the magnetic rotor 600 is improved, and the loss is reduced compared with the traditional gear transmission.

[0032] like Figure 1 , Figure 2 and Figure 4 As shown, a sealing ring 204 is fixedly connected to the bottom of the cover plate 203, a sealing groove 202 is annularly opened above the reaction cup 201, and the bottom of the sealing ring 204 is embedded in the cover plate 203 through the sealing groove 202. A support plate 400 is movably inserted into the middle of the bracket 300, one side of the electric push rod 401 is fixed on the support plate 400, and a spiral groove 601 is spirally opened on the surface of the rotor 600. The rotor 600 rotates at the bottom center of the reaction cup 201.

[0033] In this embodiment, a silicone sealing ring 204 is fixed below the cover plate 203 by a molding process. The sealing ring 204 has a trapezoidal cross-section and forms an interference fit with the annular sealing groove 202 at the top of the reaction cup 201. A sliding groove is opened in the middle of the bracket 300. The support plate 400 is connected to the sliding groove through a ball block. The bottom flange of the electric push rod 401 is locked to the support plate 400 by an M3 hexagonal screw, so that the cover plate 203 can be raised and lowered smoothly within the stroke.

[0034] The rotor 600 has double helical grooves 601 machined on its surface, which generate axial vortices when rotating, causing the reaction liquid to circulate up and down, improving the mixing efficiency compared to the single helical design. At the same time, the chamfered design of the edges of the helical grooves 601 avoids the concentration of shear force and protects the integrity of magnetic bead reagents. Magnets are embedded at the bottom of both ends of the magnetic rotor 600 and form multi-pole magnetic coupling with the annular magnetic array in the motor base 200, which improves the efficiency compared to the traditional gear transmission. This layout allows the reaction cup 201 to complete the entire process from sample addition to detection in a sealed state. The sealing pressure is dynamically adjusted by the electric push rod 401 to adapt to the packaging requirements of samples of different volumes, effectively solving the cross-contamination problem caused by open operation in traditional devices.

[0035] like Figure 1 , Figure 3and Figure 5 As shown, the feeding assembly 500 includes a sealed container 501 located on the reaction cup 201. A connecting pipe 503 is fixedly inserted into the center of the lower end of the sealed container 501. The lower end of the connecting pipe 503 passes through the cover plate 203 and is inserted into the interior of the reaction cup 201. A flexible pipe 504 and a one-way valve 502 are provided in the middle of the connecting pipe 503. The two ends of the flexible pipe 504 are sleeved on the port surface of the connecting pipe 503, and the one-way valve 502 is located below the flexible pipe 504.

[0036] In this embodiment, the sealing tank 501 of the feeding component 500 is integrally molded with PTFE material, and the tank body is corrosion resistant. The bottom of the sealing tank 501 is connected to the connecting pipe 503 through a clamp joint. The surface of the connecting pipe 503 is coated with polytetrafluoroethylene to reduce the liquid adsorption rate.

[0037] The connecting tube 503 integrates a flexible tube 504 in the middle, made of silicone material, with a certain length allowance for expansion and contraction. This, combined with the vertical displacement generated when the cover plate 203 is raised and lowered, minimizes the risk of pipe breakage. The one-way valve 502 has a built-in spring-loaded polyether ether ketone valve core. The opening pressure can prevent the reaction liquid from flowing back to the sealed container 501, while allowing the ethanol cleaning solution to backwash the pipeline. It is also linked and controlled by the electric actuator 401 to realize the full automation of the "sealing-sampling-cleaning" process, shortening the single detection cycle and greatly reducing the risk of cross-contamination.

[0038] Working principle: The motor seat 200 inside the base 100 supports the reaction cup 201 and achieves precise axial positioning with the limiting groove 101. The brushless motor drives the magnetic ring inside the disk to rotate, and through magnetic coupling, it drives the magnetic rotor 600 inside the reaction cup 201 to rotate synchronously. The double spiral groove 601 on the surface of the magnetic rotor 600 generates axial eddies, which promotes the reaction liquid to form an up-and-down circulation flow. At the same time, the chamfered edge design of the spiral groove 601 avoids shear force from damaging the magnetic bead reagent.

[0039] The height of the cover plate 203 is adjusted by the electric push rod 401. The silicone sealing ring 204 below forms an interference fit with the sealing groove 202 at the top of the reaction cup 201 to seal the reaction chamber. The sealed tank 501 of the feeding component 500 injects reagents into the reaction cup 201 through the connecting pipe 503. The flexible tube 504 adapts to the lifting and lowering displacement of the cover plate 203. The one-way valve 502 opens the pressure to prevent liquid backflow and supports ethanol backflushing of the pipeline.

[0040] The controller 205 communicates with the motor mount 200 via a flexible FPC cable to adjust the speed of the brushless motor and the sealing pressure of the electric push rod 401 in real time. This design realizes full automation of the "sample addition-stirring-detection-cleaning" process, reduces the single detection cycle, lowers the cross-contamination rate, and significantly improves the sensitivity and repeatability of immunoassays such as chemiluminescence.

[0041] It should be noted that the motor mount 200, controller 205 and electric actuator 401 mentioned above are all devices with relatively mature existing technology. The specific models can be selected according to actual needs. At the same time, the motor mount 200, controller 205 and electric actuator 401 can be powered by the built-in power supply or by the mains power. The specific power supply method is selected according to the situation and will not be elaborated here.

[0042] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. An immunobiochemical reaction device, characterized in that, include: A base (100) is provided with a motor seat (200) inside the base (100), and a reaction cup (201) is provided above the motor seat (200). A rotor (600) is provided inside the reaction cup (201) and is magnetically connected to the motor seat (200) below. A cover plate (203) is provided above the reaction cup (201). The reaction cup (201) and the cover plate (203) form a sealed reaction chamber. An electric push rod (401) is provided above the cover plate (203). A bracket (300) is provided on one side of the electric push rod (401). A feeding assembly (500) is disposed on the support (300) and located above the reaction cup (201).

2. The immunobiochemical reaction apparatus according to claim 1, characterized in that, The motor base (200) has a groove on its upper part, and the reaction cup (201) is embedded in the motor base (200) through the groove below.

3. The immunobiochemical reaction apparatus according to claim 1, characterized in that, A limiting groove (101) is provided in the middle of the base (100), and a controller (205) is electrically connected to one side of the motor base (200). One side of the controller (205) is embedded in the base (100) through the limiting groove (101).

4. The immunobiochemical reaction apparatus according to claim 1, characterized in that, A sealing ring (204) is fixedly connected to the bottom of the cover plate (203), and a sealing groove (202) is provided in an annular shape above the reaction cup (201). The bottom of the sealing ring (204) is embedded in the inside of the cover plate (203) through the sealing groove (202).

5. The immunobiochemical reaction apparatus according to claim 1, characterized in that, A support plate (400) is movably inserted into the middle of the bracket (300), and one side of the electric push rod (401) is fixed on the support plate (400).

6. The immunobiochemical reaction apparatus according to claim 1, characterized in that, The feeding assembly (500) includes a sealed container (501) located on the reaction cup (201). A connecting pipe (503) is fixedly inserted at the center of the lower end of the sealed container (501). The lower end of the connecting pipe (503) passes through the cover plate (203) and is inserted into the interior of the reaction cup (201).

7. The immunobiochemical reaction apparatus according to claim 6, characterized in that, The connecting pipe (503) has a flexible pipe (504) and a one-way valve (502) in the middle. The two ends of the flexible pipe (504) are sleeved on the port surface of the connecting pipe (503), and the one-way valve (502) is located below the flexible pipe (504).

8. The immunobiochemical reaction apparatus according to claim 1, characterized in that, The rotor (600) has a spiral groove (601) spirally opened on its surface, and the rotor (600) rotates at the bottom center of the reaction cup (201).

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