Mechanical hyperalgesia tagging adapter

Abstract

The utility model relates to biological experiment instrument technical field especially, more particularly to a kind of mechanical pain sensitivity marking adapter, comprising: adapter main body, the adapter main body is the cylindrical shape for the convenience grip, the adapter main body top end detachably fixed installation is used to stimulate mouse sole mechanical stimulation component, the adapter main body inner portion is separately provided with bluetooth module and processor, the adapter main body outer wall one side is provided with button, marking instruction is sent to processor by pressing button, processor will transmit computer by bluetooth module with marking record is completed on computer, the utility model provides a kind of mechanical pain sensitivity marking adapter, and the result credibility is high only one person operation.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental equipment technology, and in particular to a mechanical pain-sensitive marking adapter. Background Technology

[0002] With the rapid development of basic neuroscience research, in vivo fiber optic recording technology has become one of the most crucial techniques for studying the electrical activity of a group of neurons in pain-related brain regions during free movement in small animals. This technology records event-related neuronal calcium signals or neurotransmitter release. Currently, when studying pain-like behavioral phenotypes, the optical fiber at the head of the experimental mouse is connected to the fiber optic recording instrument via a jumper. The experimental mouse is placed in an independent compartment with a wire mesh underneath, allowing the mouse to move freely on the wire mesh. The experimenter can observe the animal's pain-like behaviors—licking the paw and withdrawing the paw—through the wire mesh. Recording requires close cooperation between two researchers: one manually labels the mechanical stimulation of the sole of the foot on the software, while the other holds the mechanical analgesia device and follows instructions, stimulating the sole of the foot immediately after labeling. The final effective result is the peak of the response immediately after the labeling time point.

[0003] However, current methods for mouse labeling and mechanical stimulation present several problems: 1. The experiment requires two people: the pain-measuring experimenter needs to focus on aiming at the mouse's paw, while the labeling experimenter needs to focus on the software and be ready to label at any time, making it difficult for one person to complete independently; 2. When the stimulation and labeling commands are issued simultaneously, if the experimenter approaches the mouse with the pain-measuring cilia, the mouse may move prematurely or the stimulation position may be inaccurate, resulting in asynchrony between labeling and effective stimulation; 3. A single fiber optic recording experiment often requires multiple tests on the same mouse to obtain an average. Repeated audio commands can induce fear-based conditioned responses in the animal, causing the recorded peak to occur prematurely upon hearing the audio command; 4. In the above situations, the test results often require the experimenter to manually adjust the labeling of delayed or premature events in the software later, which seriously affects the objectivity and reliability of the test results. Clearly, the current fiber optic recording process for pain-like behavior still needs to address issues such as convenience and synchronization, which are crucial to the objectivity and reliability of the final results. Utility Model Content

[0004] The purpose of this invention is to provide a mechanical pain-sensitive marking adapter to solve the technical problems existing in the background art.

[0005] To achieve the above objectives, the technical solution of this utility model is as follows:

[0006] A mechanical pain-sensitive marking adapter includes: an adapter body, which is cylindrical for easy gripping; a mechanical stimulation component for stimulating the soles of mice is detachably fixed at the top of the adapter body; a Bluetooth module and a processor are respectively disposed inside the adapter body; and a button is disposed on one side of the outer wall of the adapter body. By pressing the button, a marking command is sent to the processor, which transmits the command to a computer via the Bluetooth module and completes the marking record on the computer.

[0007] Furthermore, the adapter body has a slot at its top, and the mechanical stimulation component includes a pain-measuring handle and pain-measuring cilia. One end of the pain-measuring cilia is fixedly connected to the upper side of the pain-measuring handle, and the lower part of the pain-measuring handle is detachably inserted and fixed in the slot.

[0008] Furthermore, the slot is circular, and the pain measurement handle includes a vertical part and a horizontal part. The horizontal part is vertically fixedly connected to the upper part of the vertical part. The vertical part is circular and matches the slot. The vertical part is detachably inserted and fixed in the slot. One end of the pain measurement cilia is fixedly connected to one end of the horizontal part.

[0009] Furthermore, a number of rubber bristles are evenly arranged on the inner wall of the slot, and the diameter of the slot is larger than the diameter of the vertical part of the pain measurement handle. When the vertical part of the pain measurement handle is inserted into the slot, its outer wall is pressed into contact with the number of rubber bristles.

[0010] Furthermore, the pain-measuring cilia are Von Frey fibers.

[0011] Furthermore, it also includes: a switch and a battery, wherein the switch is disposed on one side of the outer wall of the adapter body, the battery is disposed inside the adapter body, the battery powers the Bluetooth module and the processor, and the switch is connected in series with the battery.

[0012] Furthermore, it also includes a USB interface, which is located on one side of the outer wall of the adapter body.

[0013] Furthermore, it also includes a power indicator light, which is located on one side of the outer wall of the adapter body to indicate whether the battery is charging.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] 1. Reduced labor costs. Previously, in this experiment, one experimenter would provide mechanical stimulation while another would apply labels upon receiving instructions. Now, with the pain-measuring handle inserted into the slot, the same experimenter can apply mechanical stimulation while simultaneously pressing the button to apply labels, significantly simplifying the operation and saving labor costs.

[0016] 2. It ensures the synchronization of command issuance and mechanical stimulation. When the tester holds the pain-detecting cilia close to the mouse's foot, the button can be pressed, and the foot will be stimulated instantly, maximizing the synchronization of command issuance and mechanical stimulation, and improving the effectiveness and success rate of data collection.

[0017] 3. Avoid false positive reactions in laboratory animals. Because the above procedures are performed by one person, the transmission of verbal commands between the experimenters is avoided, which significantly reduces the risk of animals developing conditioned fear responses and thus avoids false positive signals caused by fear responses.

[0018] 4. It ensures the objectivity and reliability of the acquisition results. Since the marking and mechanical stimulation are performed by the same person, the operation steps can be highly synchronized, which improves the time resolution of marking and signal response, thus ensuring the objectivity of the acquisition results. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the main body of the adapter in this utility model;

[0020] Figure 2 This is a cross-sectional view of the adapter body in this utility model;

[0021] Figure 3 This is the control circuit diagram of this utility model;

[0022] Figure 4 This is a schematic diagram of the mechanical stimulation component in this utility model.

[0023] The labels in the attached diagram are as follows: 1-Adapter body, 2-Card slot, 3-Rubber bristles, 4-Switch, 5-Power indicator light, 6-Bluetooth module, 7-Button, 8-USB interface, 9-Battery, 10-Processor, 11-Pain measurement handpiece, 12-Pain measurement bristles. Detailed Implementation

[0024] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments.

[0025] See Figures 1-4 As shown, a mechanical pain-sensitive marking adapter includes: an adapter body 1, which is cylindrical in shape for easy gripping; a mechanical stimulation component for stimulating the soles of mice is detachably fixedly installed at the top of the adapter body 1; a Bluetooth module 6 and a processor 10 are respectively arranged inside the adapter body 1; a button 7 is arranged on one side of the outer wall of the adapter body 1; by pressing the button 7, a marking command is sent to the processor 10; the processor 10 transmits the command to the computer through the Bluetooth module 6 and completes the marking record on the computer.

[0026] In this embodiment, the adapter body 1 has a slot 2 at its top. The mechanical stimulation component includes a pain-sensing handle 11 and pain-sensing cilia 12, the pain-sensing cilia 12 being Von Frey fibers. One end of the pain-sensing cilia 12 is fixedly connected to the upper side of the pain-sensing handle 11, and the lower part of the pain-sensing handle 11 is detachably inserted and fixed in the slot 2. The slot 2 is circular. The pain-sensing handle includes a vertical part and a horizontal part. The horizontal part is vertically fixedly connected to the upper part of the vertical part. The vertical part is circular and matches the slot 2. The vertical part is detachably inserted and fixed in the slot 2. One end of the pain-sensing cilia 12 is fixedly connected to one end of the horizontal part. Several rubber bristles 3 are evenly arranged on the inner wall of the slot 2. The diameter of the slot 2 is larger than the diameter of the vertical part of the pain-sensing handle. When the vertical part of the pain-sensing handle is inserted into the slot 2, its outer wall is pressed tightly against the several rubber bristles 3, preventing the pain-sensing handle 11 from loosening.

[0027] This embodiment also includes: a switch 4 and a battery 9. The switch 4 is disposed on one side of the outer wall of the adapter body 1, and the battery 9 is disposed inside the adapter body 1. The battery 9 supplies power to the Bluetooth module 6 and the processor 10. The switch 4 and the battery 9 are connected in series. It also includes: a USB interface 8, which is disposed on one side of the outer wall of the adapter body 1 as an interface for charging the battery 9. It also includes: a power indicator light 5, which is disposed on one side of the outer wall of the adapter body 1.

[0028] USB port 8 is connected in series with battery 9, switch 4, and processor 10; processor 10 is connected in parallel with power indicator 5, Bluetooth module 6, and button 7. When USB port 8 is connected to a USB data cable, it can charge battery 9. Since power indicator 5 is connected to battery 9 via processor 10, its operation indicates the charging status. When switch 4 is turned on, battery 9 supplies power to processor 10, power indicator 5, Bluetooth module 6, and button 7 in sequence. Therefore, when switch 4 is on, power indicator 5 indicates that the device is currently powered on, and Bluetooth module 6 enters a pairing waiting state. After Bluetooth module 6 is paired with a computer, marking can be performed by pressing button 7.

[0029] The application process of the mechanical pain-sensitive marking adapter is explained below:

[0030] Taking the recording of mouse responses to mechanical stimulation as an example, two weeks prior, the test mice underwent calcium signaling virus injection and fiber optic implantation in the brain regions of interest with the assistance of a stereotaxic instrument. One end of the fiber optic cable was implanted inside the nucleus, and the other end was fixed to the surface of the mouse skull with dental cement. Before the test, the fiber optic recording instrument was connected to a computer, with one end of a jumper cable connected to the recording instrument. The recording software and instrument were opened, and the test parameters were adjusted. The other end of the jumper cable was connected to the surface of the mouse skull via the fiber optic cable. The mouse was placed in an isolated compartment with a wire mesh at the bottom. Through the bottom of the wire mesh, the operator could observe the details of the mouse's foot movements.

[0031] After the mouse has calmed down completely, turn on switch 4. The battery 9 will then power the internal components of the main unit, and the power indicator light 5 will illuminate to indicate that the device is powered on. Simultaneously, the Bluetooth module 6 will turn on and enter a connection waiting state. After the Bluetooth module 6 is connected to the recording computer, move the mouse cursor to the marking button on the screen. This completes all preparations.

[0032] Taking a 0.16g force stimulation of the mouse's right hind paw as an example, the operator first assembles the pain-measuring handle 11 by embedding it into the groove 2. Holding the 0.16g pain-measuring ciliary 12 with the thumb on the surface of button 7, the operator presses button 7 when the ciliary 12 is vertically aimed at and close to the mouse's right hind paw. Button 7 sends a click command to the computer via the processor 10 and Bluetooth module 6, resulting in a mark appearing on the recording software. Simultaneously, the operator quickly raises their arm, causing the pain-measuring ciliary 12 to contact the paw until it fully flexes, completing the mechanical stimulation. If the mouse exhibits a paw withdrawal response, it indicates that 0.16g is sufficient to elicit a pain-like response. The recording software also displays an event-response related calcium signal waveform, reflecting the neuronal activity in the brain region of interest under 0.16g force stimulation. To compare the neuronal responses of mice under different weights of pain-measuring ciliary stimulation, simply remove the pain-measuring handle and insert a handle of the next weight; details are omitted here.

[0033] In the description of this utility model, it should be noted that the terms "upper", "lower", "left", "right", "inner", "outer", "top / bottom", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0034] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any way. Any simple modifications, equivalent substitutions, and improvements made by those skilled in the art to the above embodiments without departing from the scope of the present utility model's technical solution and based on the technical essence of the present utility model shall still fall within the protection scope of the present utility model's technical solution.

Claims

1. A mechanical pain-sensitive marking adapter, characterized in that, include: The adapter body (1) is a cylindrical shape that is easy to hold. The top of the adapter body (1) is detachably fixed with a mechanical stimulation component for stimulating the soles of mice. The adapter body (1) is equipped with a Bluetooth module (6) and a processor (10) respectively. A button (7) is provided on one side of the outer wall of the adapter body (1). By pressing the button (7), a marking instruction is sent to the processor (10). The processor (10) transmits the instruction to the computer through the Bluetooth module (6) and completes the marking record on the computer.

2. The mechanical pain-sensitive marking adapter according to claim 1, characterized in that: The adapter body (1) has a slot (2) at the top. The mechanical stimulation component includes a pain-measuring handle (11) and a pain-measuring cilia (12). One end of the pain-measuring cilia (12) is fixedly connected to the upper side of the pain-measuring handle (11). The lower part of the pain-measuring handle (11) is detachably inserted and fixed in the slot (2).

3. The mechanical pain-sensitive marking adapter according to claim 2, characterized in that: The slot (2) is circular. The pain measurement handle includes a vertical part and a horizontal part. The horizontal part is vertically fixed to the upper part of the vertical part. The vertical part is circular and matches the slot (2). The vertical part is detachably inserted and fixed in the slot (2). One end of the pain measurement cilia (12) is fixedly connected to one end of the horizontal part.

4. A mechanical pain-sensitive marking adapter according to claim 3, characterized in that: The inner wall of the slot (2) is evenly provided with several rubber bristles (3). The diameter of the slot (2) is larger than the diameter of the vertical part of the pain measurement handle. When the vertical part of the pain measurement handle is inserted into the slot (2), its outer wall is pressed and contacted with several rubber bristles (3).

5. A mechanical pain-sensitive marking adapter according to claim 2, characterized in that: The pain-measuring cilia (12) are Von Frey fibers.

6. A mechanical pain-sensitive marking adapter according to claim 1, characterized in that: Also includes: A switch (4) and a battery (9) are provided. The switch (4) is located on one side of the outer wall of the adapter body (1), and the battery (9) is located inside the adapter body (1). The battery (9) supplies power to the Bluetooth module (6) and the processor (10). The switch (4) and the battery (9) are connected in series.

7. A mechanical pain-sensitive marking adapter according to claim 6, characterized in that: Also includes: USB interface (8), which is located on one side of the outer wall of the adapter body (1) as an interface for charging the battery (9).

8. A mechanical pain-sensitive marking adapter according to claim 6, characterized in that: Also includes: Power indicator light (5) is located on one side of the outer wall of the adapter body (1).

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