In-situ isotope tracing soil incubation device

Abstract

The utility model discloses a kind of isotopic tracing soil culture devices in situ in field, solve the problems such as high isotope loss of traditional device, environmental simulation distortion and cumbersome operation etc..The isotopic tracing soil culture device, including split type pipe body, by split type pipe body using left, right semicircular pipe body's movable inlay, reduce the structure damage to in-situ soil when sampling;By setting upper end cap and lower end cap respectively in the upper and lower ends of split type pipe body, fixed split type pipe body also make the closed circulation inside culture tube, prevent isotope leakage.Several through-holes are set in the pipe wall of split type pipe body and corresponding filter membrane is provided to realize the distribution and diffusion of isotope control;By setting lifting component, manual use is facilitated.

Description

Technical Field

[0001] This utility model relates to the field of isotope in-situ soil culture, specifically to an isotope tracing soil culture device for in-situ field use. Background Technology

[0002] Isotope tracing techniques (such as) ¹³ C 15 N、 14 (C, etc.) By labeling target elements with nuclides, the dynamic tracking of their migration, transformation, and metabolic processes in soil, plants, and the environment has become an indispensable research tool in agricultural science. In soil and fertilization science, this technology can accurately analyze fertilizer absorption and utilization rates, soil nutrient dynamics, organic matter decomposition mechanisms, and pollutant migration patterns, providing crucial data support for optimizing fertilization strategies, improving resource utilization efficiency, and assessing environmental risks. For example, the "A-value method" can be used to determine the available phosphorus content in soil, or... 15 Nitrogen (N) labeling studies on nitrogen fertilizer leaching and volatilization pathways have significantly promoted the development of precision agriculture and sustainable soil management. Furthermore, isotope tracing technology has been used to reveal the microbial degradation mechanisms of straw carbon in soil and its carbon sequestration potential, providing a scientific basis for addressing climate change.

[0003] While existing isotope tracing technology has significant value in agriculture and soil science, its underlying devices and methods suffer from core drawbacks such as structural redundancy, distorted environmental simulation, severe isotope depletion, and excessively high costs. There is an urgent need to develop an innovative device that combines in-situ environmental simulation, efficient isotope utilization, accuracy and reliability, and low-cost adaptability to overcome the technological bottlenecks in soil ecological research and precision agriculture.

[0004] The information disclosed in this background section is intended only to enhance the understanding of the background technology of this disclosure and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0005] The inventors discovered through research that traditional in-situ soil culture devices have simple structures and limited functions, making it difficult to fill the devices with in-situ soil, inconvenient to set up in the field, and difficult to collect samples subsequently; at the same time, there are problems such as serious isotope loss and inaccurate experimental results.

[0006] In view of at least one of the above technical problems, this disclosure provides an isotope tracing in-situ soil culture device, including a culture tube. The device solves the problems of high isotope loss, environmental simulation distortion and cumbersome operation of traditional devices by combining a split tube body, through holes and a first filter membrane, an upper end cover and a filter screen, a lower end cover and a second filter membrane. It is especially suitable for in-situ research on sensitive ecosystems such as paddy fields and wetlands.

[0007] According to one aspect of this disclosure, a field in-situ isotope tracing soil culture device is provided, comprising a culture tube, the culture tube including a split tube body, an upper end cap detachably connected to the upper port of the split tube body, and a lower end cap detachably connected to the lower port of the split tube body. The split tube body provides soil experimental space for isotopes while the split design reduces mechanical damage to the soil during sampling, ensuring the integrity of the soil structure. The tube wall of the split tube body is provided with a lifting component and several through holes. The lifting component enables portable access to the culture tube, the through holes enable uniform diffusion of isotopes, and a reverse osmosis membrane controls isotope exchange between the inside and outside of the culture tube, thereby improving the accuracy of the experiment. A first filter membrane is provided on the inner surface of the through holes, a filter screen is provided on the upper end cap corresponding to the upper port of the split tube body, and a second filter membrane is provided on the lower end cap corresponding to the lower port of the split tube body.

[0008] In some embodiments of this disclosure, the split tube body includes a left semicircular tube body and a right semicircular tube body, which are movably fitted together by a mortise and tenon structure, making the fit simple and the disassembly and assembly convenient.

[0009] In some embodiments of this disclosure, the mortise and tenon structure includes a trapezoidal groove disposed at the joint of the left semicircular pipe and a trapezoidal protrusion disposed at the joint of the right semicircular pipe and movably fitted with the trapezoidal groove. A leak-proof step is provided at the joint of the left and right semicircular pipes. The leak-proof step improves the airtightness of the split pipe body and prevents water and fertilizer leakage at the joint of the left and right semicircular pipes.

[0010] In some embodiments of this disclosure, the two ends of the split tube are provided with external threads and are respectively threaded to the upper end cap and the lower end cap, so that the interior of the split tube forms a closed loop, preventing isotope leakage, while the left and right semicircular tubes of the split tube are tightly spliced ​​together, improving the airtightness of the culture tube and the integrity of the device.

[0011] In some embodiments of this disclosure, the filter mesh is a stainless steel woven mesh, and the second filter membrane is a nylon microporous filter membrane. The stainless steel woven mesh provides insect prevention, ventilation, and light transmission, intercepting large particulate impurities and preventing blockage of the separate culture tubes, thereby ensuring continuous isotope permeation. The nylon microporous filter membrane prevents soil particle loss while allowing air and moisture exchange, thus maintaining a stable microenvironment within the culture tubes.

[0012] In some embodiments of this disclosure, the plurality of through holes are arranged circumferentially at 5 cm intervals on the lower tube wall of the split tube, and the first filter membrane is a reverse osmosis membrane. The reverse osmosis membrane controls the isotope exchange between the inside and outside of the culture tube, thereby improving the accuracy of the experiment.

[0013] In some embodiments of this disclosure, the lifting component includes a detachably connected flexible support belt, which is secured to the wall of the split-type pipe body by at least two anchors. The flexible support belt is adaptable to rapid loading, unloading, and transportation under different terrain conditions, offering convenient operation.

[0014] In some embodiments of this disclosure, the flexible load-bearing belt is a nylon braided belt or a silicone-coated steel wire rope, which is strong and durable.

[0015] One or more technical solutions provided in the embodiments of this application have at least one of the following technical effects or advantages:

[0016] 1. By adopting a movable interlocking scheme based on a mortise and tenon structure for the left and right semi-circular tubes, the rigidity limitation of traditional integrated tubes is overcome. The mortise and tenon structure, combined with the external thread design of the upper and lower ports of the split tubes, enables rapid and sealed connection and disassembly of the split tubes and the upper and lower end caps, facilitating the non-destructive collection and acquisition of tracer soil inside the culture tubes and improving the in-situ authenticity of isotope labeling. At the same time, it significantly reduces the time spent on device assembly and cleaning, supports reuse, and reduces the cost of experimental consumables. By setting steps and an impermeable membrane lining at the joint, a multi-level sealing barrier is formed, effectively suppressing the lateral leakage of isotope labeling and interference from the external environment.

[0017] 2. By setting stainless steel woven mesh and nylon microporous filter membranes at the upper and lower ends of the corresponding split tube body on the upper and lower end caps, a closed circulation is formed inside the culture tube, allowing air and water exchange while preventing soil particles from overflowing, thereby maintaining the stability of the microenvironment inside the culture tube.

[0018] 3. By arranging several circumferentially distributed through holes in the split tube wall and installing a reverse osmosis membrane inside, a selective material exchange channel is formed, realizing the exchange of soil water and air inside and outside, while controlling the leakage of isotopes inside the culture tube and improving the accuracy of the experiment; by setting up a lifting component to adapt to rapid loading and unloading and transportation under different terrain conditions, the operation is convenient. Attached Figure Description

[0019] Figure 1 This is a front view of a culture tube according to an embodiment of this application.

[0020] Figure 2 This is a cross-sectional view of a culture tube according to an embodiment of this application.

[0021] Figure 3 This is a schematic diagram of the structure of a split tube body and upper and lower end caps according to an embodiment of this application.

[0022] In the above figures, 1 is a split tube body, 2 is the upper end cap, 3 is the lower end cap, 4 is the lifting component, 5 is the through hole, 6 is the first filter membrane, 7 is the filter screen, 8 is the second filter membrane, 9 is the left semi-circular tube body, 10 is the right semi-circular tube body, 11 is the trapezoidal groove, 12 is the trapezoidal protrusion, 13 is the anti-leakage step, 14 is the external thread, 15 is the flexible bearing band, and 16 is the anchor. Detailed Implementation

[0023] In the description of this application, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," "vertical," "horizontal," "clockwise," and "counterclockwise," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the terms "connection" and "linkage" in this application, unless otherwise specified, include both direct and indirect connections (linkages).

[0024] Unless otherwise specified, the unit modules, components, structures, mechanisms, or sensors involved in the following embodiments are all commercially available products.

[0025] This application provides an automatic tilting and erecting machine device, which solves the problems of high isotope loss, environmental simulation distortion, and cumbersome operation of traditional devices.

[0026] The technical solution in this application is to solve the above problems, and the overall approach is as follows:

[0027] The problem of traditional culture devices easily damaging the soil structure and causing microenvironment disturbance is solved by setting up a split tube body 1; the distribution and diffusion of isotopes are controlled by setting several through holes 5 in the tube wall of the split tube body 1 and lining it with a first filter membrane 6; the split tube body 1 is fixed by setting threaded upper end cap 2 and lower end cap 3 while controlling the closed circulation of isotopes; and the lifting component 4 set on the tube wall of the split tube body 1 facilitates manual use.

[0028] To better understand the technical solution of this application, the above technical solution will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0029] Example 1

[0030] This example discloses an in-situ isotope tracing soil culture device for field use, such as Figure 1 , 2As shown in Figure 3, the culture tube includes a split tube body 1, an upper end cap 2 detachably connected to the upper port of the split tube body 1, and a lower end cap 3 detachably connected to the lower port of the split tube body 1. The split tube body 1 provides soil experimental space for isotopes while its split design reduces mechanical damage to the soil during sampling, ensuring the integrity of the soil structure. The tube wall of the split tube body 1 is provided with a lifting component 4 and several through holes 5. The lifting component 4 enables portable access to the culture tube. Preferably, the several through holes 5 are arranged circumferentially at 5cm intervals on the lower tube wall of the split culture tube 1, allowing the through holes 6 to be buried in the soil to prevent surface runoff from affecting the soil inside the tube. A first filter membrane 6 is provided on the inner surface of the through holes 5. Preferably, the first filter membrane 6 is a reverse osmosis membrane, and its edges are fixed to the periphery of the through holes by heat fusion. A series of equally spaced through-holes 5 enable uniform diffusion of isotopes, while the reverse osmosis membrane controls isotope exchange between the inside and outside of the culture tube, thereby improving the accuracy of the experiment. The upper end cap 2 is equipped with a filter screen 7 at the upper port of the split-type tube 1, and the lower end cap 3 is equipped with a second filter membrane 8 at the lower port of the split-type tube 1. Preferably, the filter screen 7 is a stainless steel woven mesh with a pore size of 0.5~1mm, and the second filter membrane 8 is a nylon microporous filter membrane with a pore size of 0.2~0.5μm. The stainless steel woven mesh, positioned at the upper port of the split-type tube 1, provides insect prevention, ventilation, and light transmission, intercepting large particles and preventing blockage of the split-type culture tube 1, thus ensuring continuous isotope permeation. The nylon microporous filter membrane, positioned at the lower port of the split-type tube 1, prevents soil particle loss while allowing air and moisture exchange, thereby maintaining a stable microenvironment within the culture tube. Preferably, when soil or isotope solution is poured into the culture tube, a thick-necked funnel can be placed at the upper end of the split tube 1 to facilitate introduction and simplify the operation process.

[0031] Furthermore, such as Figure 1 As shown, the split-type pipe body 1 includes a left semicircular pipe body 9 and a right semicircular pipe body 10. Preferably, the split-type pipe body 1 is a PVC pipe with a diameter of 5-10cm, which is inexpensive, sturdy, and durable. The left semicircular pipe body 9 and the right semicircular pipe body 10 are movably fitted together by a mortise and tenon structure. The mortise and tenon structure includes a trapezoidal groove 11 set at the joint of the left semicircular pipe body 9 and a trapezoidal protrusion 12 set at the joint of the right semicircular pipe body 10 and movably fitted with the trapezoidal groove 11, which is simple to fit and easy to assemble and disassemble. A leak-proof step 13 is provided at the joint of the left semicircular pipe body 9 and the right semicircular pipe body 10 to improve the airtightness of the split-type pipe body 1 and prevent water and fertilizer leakage at the joint of the left and right semicircular pipe bodies. Preferably, a waterproof membrane can also be lined on the inner wall of the split-type pipe body 1 to further enhance the leak-proof effect.

[0032] Specifically, the left semicircular tube 9 and the right semicircular tube 10 of the split-type tube 1 are movably fitted together using a mortise and tenon structure. According to experimental requirements, the culture tubes can be assembled after being pre-grooved in the soil at the experimental site to prevent disturbance to the microenvironment during soil sampling, reduce human disturbance, ensure the integrity of the soil pore structure and redox state, and improve the in-situ authenticity of isotope labeling. Furthermore, the split-type tube 1 can be easily disassembled and cleaned after the experiment, allowing for multiple reuses and application in different experimental scenarios, resulting in a long service life and strong adaptability.

[0033] Furthermore, such as Figure 1 , 3 As shown, the upper and lower ends of the split tube 1 are provided with external threads 14, which are threaded to the upper and lower end caps respectively, forming a closed loop inside the split tube 1. This prevents isotope leakage and ensures that the left semicircular tube 9 and right semicircular tube 10 of the split tube 1 are tightly joined, improving the airtightness of the culture tube and the overall integrity of the device. Preferably, an annular filter screen fixing frame can be embedded on the inner side of the upper and lower end caps, and the corresponding filter screen or filter membrane can be fixed by a snap-fit ​​structure.

[0034] Furthermore, such as Figure 2 As shown, the lifting component includes a detachably connected flexible support belt 15, which is fixed to the wall of the split-type pipe body 1 by two anchors 16. The flexible support belt 15 is adaptable to rapid loading, unloading, and transportation under different terrain conditions, and is easy to operate. Preferably, the flexible support belt is a nylon braided belt or a silicone-coated steel wire rope, and both ends of the nylon braided belt or silicone-coated steel wire rope are provided with buckles that cooperate with the anchors 16 to achieve quick insertion and removal.

[0035] The method of using this device is as follows:

[0036] According to the experimental requirements, the first procedure is as follows: Assemble the split-type tube 1 and tighten the lower end cap 3. Place a thick-necked funnel at the upper end of the split-type tube 1. Line the split-type tube 1 with an additional impermeable membrane. Then, mix the in-situ soil from the experimental site with the isotopes and pour the mixture into the tube through the thick-necked funnel. Remove the thick-necked funnel and tighten the upper end cap 2. Use the flexible support belt 15 to carry the culture tube to the experimental site and bury it in the soil. After the experiment, retrieve the culture tube using the flexible support belt 15. After obtaining the experimental data, disassemble the split-type tube 1 for cleaning to facilitate future use.

[0037] Operation 2: First, dig a trench in the soil of the experimental site, then assemble the left semicircular tube 9 and right semicircular tube 1 of the split-type tube body 1. Tighten the upper end cap 2 and the lower end cap 3. After bringing the in-situ soil back, pour the isotope solution into the upper port of the split-type tube body 1 through a thick-necked funnel. Remove the thick-necked funnel and tighten the upper end cap 2. Use the flexible support belt 15 to carry the culture tube to the experimental site and bury it in the soil. After the experiment, retrieve the culture tube through the flexible support belt 15, obtain the experimental data, disassemble the split-type tube body 1 for cleaning, and facilitate the next use.

[0038] Although some preferred embodiments of the present invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of this application.

[0039] Obviously, those skilled in the art can make various modifications and variations to this disclosure without departing from the spirit and scope of its inventive concept. Therefore, if such modifications and variations to this disclosure fall within the scope of the claims of this application and their equivalents, this application also intends to include such modifications and variations.

Claims

1. A field-based in-situ isotope tracing soil culture device, comprising a culture tube, characterized in that, The culture tube includes a split tube body, an upper end cap detachably connected to the upper port of the split tube body, and a lower end cap detachably connected to the lower port of the split tube body. The tube wall of the split tube body is provided with a lifting component and several through holes. The inner surface of the through holes is provided with a first filter membrane. The upper end cap is provided with a filter screen corresponding to the upper port of the split tube body, and the lower end cap is provided with a second filter membrane corresponding to the lower port of the split tube body.

2. The isotope-tracing soil culture device according to claim 1, characterized in that, The split-type tube body includes a left semicircular tube body and a right semicircular tube body, which are movably fitted together by a mortise and tenon structure.

3. The isotope-tracing soil culture device according to claim 2, characterized in that, The mortise and tenon structure includes a trapezoidal groove at the joint of the left semicircular tube and a trapezoidal protrusion at the joint of the right semicircular tube that is movably fitted with the trapezoidal groove. The joint of the left and right semicircular tubes is provided with a leak-proof step.

4. The isotope-tracing soil culture device according to claim 3, characterized in that, The split-type tube has external threads at both ends, which are threaded to the upper end cap and the lower end cap, respectively.

5. The isotope-tracing soil culture device according to claim 4, characterized in that, The filter screen is a stainless steel woven mesh, and the second filter membrane is a nylon microporous filter membrane.

6. The isotope-tracing soil culture device according to claim 1, characterized in that, The plurality of through holes are arranged circumferentially at 5cm intervals on the lower tube wall of the split tube body, and the first filter membrane is a reverse osmosis membrane.

7. The isotope-tracing soil culture device according to claim 1, characterized in that, The lifting component includes a detachably connected flexible support belt, which is fixed to the wall of the split-type tube by at least two anchors.

8. The isotope-tracing soil culture device according to claim 7, characterized in that, The flexible load-bearing belt is a nylon braided belt or a silicone-coated steel wire rope.

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