Dendrobium nobile tissue culture and epiphyte integrated device

Abstract

This invention relates to an integrated tissue culture and epiphytic device for Dendrobium officinale. It addresses the problem of plant separation or root damage during transplanting of Dendrobium officinale plants obtained through tissue culture. The main body of this integrated tissue culture and epiphytic device is composed of planar sidewalls and arc-shaped sidewalls. This device can be used for both tissue culture and epiphytic cultivation of Dendrobium officinale. When used for tissue culture, the roots of adjacent seedlings will not become entangled, avoiding root damage caused by separation during transplanting. When used for tissue culture, the device is easily connected to the seedlings by threading ropes through the hanging loops and binding them to the trunk for transplanting. Furthermore, the planar sidewalls are made of large-pore mesh, allowing roots to pass through the mesh and contact the epiphyte, which improves the survival rate of epiphytic seedlings.

Description

Technical Field

[0001] This utility model relates to a tissue culture epiphytic device for Dendrobium officinale. Background Technology

[0002] Dendrobium officinale Kimura et Migo is a valuable medicinal herb belonging to the epiphytic orchid family. It mainly grows in most areas of southern my country's tropical and subtropical monsoon regions, with a potential distribution area of ​​193 × 10⁻⁶. 4 km 2 Dendrobium officinale is renowned as the top of the "Nine Great Chinese Herbs" due to its remarkable effects on the stomach and throat, including nourishing the body, promoting saliva production, clearing heat and nourishing yin, improving eyesight and soothing the throat, enhancing immunity, anti-oxidation, lowering blood sugar, lowering blood lipids, and preventing and fighting cancer. It also has the effect of lowering blood sugar and is effective in treating gastrointestinal diseases and cataracts. Its main active ingredients are polysaccharides, dendrobine, and amino acids. Therefore, Dendrobium officinale is highly favored as a fresh food, health product, or in traditional Chinese medicine formulations.

[0003] Dendrobium officinale prefers cool, moist environments and typically grows epiphytically on high-altitude cliffs, tree trunks in forests, and in rock crevices. Due to its low natural reproductive capacity, slow growth, and long-term harvesting, wild resources of Dendrobium officinale are on the verge of depletion.

[0004] The emerging method of rapid propagation through tissue culture has solved the problem of the shortage of Dendrobium officinale seedlings. Therefore, improving and mastering the cultivation technology of Dendrobium officinale tissue culture seedlings is the foundation for large-scale artificial cultivation. Dendrobium officinale tissue culture utilizes the principle of plant cell totipotency, using aseptic techniques to inoculate callus tissue of Dendrobium officinale onto an artificially controlled culture medium, allowing it to germinate and then propagate the plants. However, because the root systems of multiple plants obtained through tissue culture are intertwined during propagation, plant separation or root damage may occur during transplanting. Furthermore, when using tissue culture-derived plants for epiphytic cultivation, the plants are often tied and fixed, making the transplanting process complex. Utility Model Content

[0005] To address the problem of plant separation or root damage during transplantation of Dendrobium officinale plants obtained through tissue culture, this invention proposes an integrated tissue culture and epiphytic device for Dendrobium officinale.

[0006] The main body of this utility model's integrated tissue culture and epiphytic device for Dendrobium officinale is composed of planar sidewalls and arc-shaped sidewalls. The upper and lower ends of the main body are open, and the upper end of the main body is provided with a constricted neck, the upper end of which is the upper port of the device. Multiple sleeves are provided on the inner surface of the arc-shaped sidewalls, the sleeves are arranged vertically, and a support rod is provided inside the sleeve. The two ends of the arc-shaped sidewalls are symmetrically provided with hanging ears. The arc-shaped sidewalls and the constricted neck are made of small-pore mesh, and the planar sidewalls are made of large-pore mesh.

[0007] The principle and beneficial effects of this utility model are as follows:

[0008] This utility model relates to an integrated tissue culture and epiphytic device for Dendrobium officinale, which can be used for both tissue culture and epiphytic cultivation of Dendrobium officinale plants. For tissue culture, callus tissue is inoculated into the main body of the device, which is then placed in a culture medium. Multiple integrated tissue culture and epiphytic devices can be placed in one culture medium. The planar sidewalls of adjacent devices face the same direction, thus their planar and arc-shaped sidewalls are adjacent. Because the arc-shaped sidewalls are made of a porous mesh, the roots of the cultured seedlings cannot penetrate them, preventing root entanglement and avoiding root damage caused by root separation during transplanting. Simultaneously, multiple sleeves are provided on the inner surface of the arc-shaped sidewalls. These sleeves are vertically arranged and contain support rods, which support the main body of the device and the root seedlings. When used for tissue culture of Dendrobium officinale plants, the support rod is removed, one side of the flat sidewall faces the epiphyte such as the trunk, and then the device is connected to the seedling by passing a rope through the hanging ear. The transplanting process is simple, and the flat sidewall is a large-pore mesh, which allows the roots to pass through the mesh and make close contact with the epiphyte, which is beneficial to improving the survival rate of seedling epiphytic cultivation. Attached Figure Description

[0009] Figure 1 This is a schematic diagram of the integrated tissue culture and epiphytic device for Dendrobium officinale in an embodiment.

[0010] Figure 2 for Figure 1 Sectional view at point AA. Detailed Implementation

[0011] The technical solution of this utility model is not limited to the specific embodiments listed below, but also includes any reasonable combination of the specific embodiments.

[0012] Specific Implementation Method 1: The main body of the Dendrobium officinale tissue culture epiphytic integrated device in this implementation method is composed of a planar side wall (1) and an arc-shaped side wall (2); the upper and lower ends of the main body are open, the upper end of the main body is provided with a constricted neck (3), the upper end of the constricted neck (3) is the upper port (7) of the device, the inner surface of the arc-shaped side wall (2) is provided with multiple sleeves (4), the sleeves (4) are arranged vertically, and a support rod (5) is provided inside the sleeves (4); the two ends of the arc-shaped side wall (2) are provided with symmetrically arranged hanging ears (6); the arc-shaped side wall (2) and the constricted neck (3) are small-pore mesh; the planar side wall (1) is large-pore mesh.

[0013] This embodiment has the following beneficial effects:

[0014] This embodiment of the Dendrobium officinale tissue culture and epiphytic integrated device can be used for both tissue culture of Dendrobium officinale plants and epiphytic cultivation of Dendrobium officinale. When used for tissue culture of Dendrobium officinale plants, callus tissue of Dendrobium officinale is inoculated into the main body of the device, and the main body of the device is placed in the culture medium. Multiple Dendrobium officinale tissue culture and epiphytic integrated devices can be placed in one culture medium. The planar sidewalls (1) of adjacent devices face the same direction. Therefore, the planar sidewalls (1) and the arc-shaped sidewalls (2) of adjacent devices are adjacent. Since the arc-shaped sidewalls (2) are made of small-pore mesh, the roots of the cultured seedlings cannot penetrate it. Therefore, the roots of adjacent seedlings will not become entangled, avoiding root damage caused by root separation during transplanting. At the same time, multiple sleeves (4) are provided on the inner surface of the arc-shaped sidewalls (2). The sleeves (4) are arranged vertically and support rods (5) are provided inside the sleeves (4), which can support the main body of the device and the root seedlings. When used for tissue culture of Dendrobium officinale plants, the support rod (5) is removed, and one side of the flat sidewall (1) is turned toward the epiphyte, such as the trunk. Then, the device is connected by a rope through the hanging ear (6) and the seedling is tied to the trunk. The transplanting process is simple, and the flat sidewall (1) is a large-pore mesh, which allows the roots to pass through the mesh and make close contact with the epiphyte, which is beneficial to improving the survival rate of seedling epiphytic cultivation.

[0015] Specific Implementation Method Two: This implementation method differs from Specific Implementation Method One in that the hanging ear (6) is an L-shaped plastic hanging ear.

[0016] Specific implementation method three: This implementation method differs from specific implementation method one or two in that: the hanging ear (6) is provided with a rope hole.

[0017] Specific Implementation Method Four: This implementation method differs from one of the specific implementation methods one to three in that the aperture of the mesh on the small-pore mesh is 0.1 to 0.3 mm.

[0018] Specific Implementation Method 5: This implementation method differs from Specific Implementation Methods 1 to 4 in that the aperture of the mesh on the large-pore mesh is 2 to 3 mm.

[0019] Specific Implementation Method Six: This implementation method differs from Specific Implementation Methods One to Five in that both the small-pore mesh and the large-pore mesh are PVC mesh.

[0020] Example 1

[0021] This embodiment of the Dendrobium officinale tissue culture and epiphytic integrated device can be used for both tissue culture of Dendrobium officinale plants and epiphytic cultivation of Dendrobium officinale. The main body of this embodiment of the Dendrobium officinale tissue culture and epiphytic integrated device is composed of a planar sidewall (1) and an arc-shaped sidewall (2); the upper and lower ends of the main body of the device are open, and the upper end of the main body of the device is provided with a constricted neck (3), the upper end of the constricted neck (3) is the upper port (7) of the device, the arc-shaped sidewall (2) and the constricted neck (3) are small-pore mesh, and the mesh diameter of the small-pore mesh is 0.1mm; the planar sidewall (1) is a large-pore mesh, and the mesh diameter of the large-pore mesh is 3mm; both the small-pore mesh and the large-pore mesh are PVC mesh; the inner surface of the arc-shaped sidewall (2) is provided with multiple sleeves (4), the sleeves ( 4) Vertically set, with a support rod (5) inside the sleeve (4); When used for tissue culture of Dendrobium officinale plants, the callus tissue of Dendrobium officinale is inoculated into the main body of the device, and the main body of the device is placed in the culture medium. Multiple Dendrobium officinale tissue culture epiphytic devices can be placed in one culture medium. The planar sidewalls (1) of adjacent devices face the same direction. Therefore, the planar sidewalls (1) and arc-shaped sidewalls (2) of adjacent devices are adjacent. Since the arc-shaped sidewall (2) is a small-pore mesh, the roots of the cultured seedlings cannot penetrate it. Therefore, the roots of adjacent seedlings will not entangle, avoiding root damage caused by root separation during transplanting. At the same time, multiple sleeves (4) are set on the inner surface of the arc-shaped sidewall (2). The sleeves (4) are set vertically and the support rods (5) are set inside the sleeves (4), which can support the main body of the device and the root seedlings. The arc-shaped sidewall (2) is provided with symmetrical hanging ears (6) at both ends. The hanging ears (6) are L-shaped plastic hanging ears with rope holes. When used for tissue culture of Dendrobium officinale plants, the support rod (5) is removed, and one side of the flat sidewall (1) is oriented towards the epiphyte, such as the trunk. Then, the device is connected by a rope through the hanging ear (6) and the seedling is tied to the trunk. The transplanting process is simple. The flat sidewall (1) is a large-pore mesh, and the roots can pass through the mesh and make close contact with the epiphyte, which is conducive to improving the survival rate of seedling epiphytic cultivation.

Claims

1. A device for integrating tissue culture and epiphytic growth of Dendrobium officinale, characterized in that: The main body of the Dendrobium officinale tissue culture epiphytic integrated device is composed of a planar side wall (1) and an arc-shaped side wall (2); the upper and lower ends of the main body are open, and the upper end of the main body is provided with a constricted neck (3), the upper end of the constricted neck (3) is the upper port (7) of the device, and multiple sleeves (4) are provided on the inner surface of the arc-shaped side wall (2). The sleeves (4) are arranged vertically, and a support rod (5) is provided inside the sleeve (4); the two ends of the arc-shaped side wall (2) are provided with symmetrically arranged hanging ears (6); the arc-shaped side wall (2) and the constricted neck (3) are made of small-pore mesh; the planar side wall (1) is made of large-pore mesh.

2. The Dendrobium nobile tissue culture epiphyte integrated device according to claim 1, characterized in that: The hanging ear (6) is an L-shaped plastic hanging ear.

3. The Dendrobium nobile tissue culture epiphyte integrated device according to claim 1, characterized in that: The loop (6) is provided with a rope hole.

4. The integrated tissue culture and epiphytic device for Dendrobium officinale according to claim 1, characterized in that: The aperture of the mesh on the small-pore mesh is 0.1 to 0.3 mm.

5. The integrated tissue culture and epiphytic device for Dendrobium officinale according to claim 1, characterized in that: The aperture of the mesh on the large-pore mesh is 2-3 mm.

6. The integrated tissue culture and epiphytic device for Dendrobium officinale according to claim 1, characterized in that: Both the small-pore and large-pore mesh fabrics are PVC mesh.

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