A polypeptide elution device

Abstract

This utility model relates to the field of peptide elution technology and discloses a peptide elution device, including a shell. A rotating mechanism is installed inside the shell to improve the stirring effect of the peptides. The stirring mechanism is also installed inside the shell to improve the elution effect of the peptides. The rotating mechanism includes a rotating column, which is installed on the inner bottom of the shell. A gear is fixedly connected to the top of the rotating column, and a gear is meshed with the right side of the gear. A cylinder is fixedly connected to the top of the gear. In this utility model, the rotating column rotates with the gear, and the gear meshes with the gear, transmitting power to the rotating column. The rotating column drives the cylinder to rotate, feeding the peptide to be eluted into the cylinder through the feed inlet. The rotation of the cylinder ensures thorough mixing of the substance with the eluent, improving the elution efficiency through mechanical stirring.

Description

Technical Field

[0001] This utility model relates to the field of peptide elution technology, and in particular to a peptide elution device. Background Technology

[0002] A peptide elution apparatus is used in the separation and purification of peptides to elute peptides adsorbed on a stationary phase. It is widely used in peptide production and scientific research. By selecting a suitable elution solvent, the adsorption between the peptide and the stationary phase is broken by utilizing the interaction forces between the solvent and the peptide, such as hydrogen bonds and van der Waals forces. This allows the peptide to separate from the stationary phase and dissolve in the eluent, thus achieving peptide elution. The main structure includes a separation column, an eluent delivery system, and a detector.

[0003] In existing technologies, the elution device is used to clean peptide raw materials during the elution process. The cleaning water contains residual impurities, and water containing impurities remains on the surface of the raw materials after cleaning, affecting the cleaning effect. Existing technologies use a design to prevent extrusion and breakage of the unblocking mechanism. After the raw materials are brushed, the cleaning water is discharged through the cleaning cylinder, and then the cleaning cylinder is driven to rotate. Centrifugal force is used to throw off the residual water and impurities on the surface of the raw materials, thereby separating the raw materials from the impurities. It can also unblock impurities that clog the filter pores. However, it relies on complex mechanical structures or control systems, such as magnetic rings driving steel rings and vacuum pumps for pressurization, which increases the risk of equipment failure and maintenance costs. Utility Model Content

[0004] To overcome the above shortcomings, this invention provides a peptide elution device, which aims to improve the problem that existing technologies rely on complex mechanical structures or control systems, such as magnetic rings driving steel rings or vacuum pumps for pressurization, which increase the risk of equipment failure and maintenance costs.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a polypeptide elution device, comprising a shell, a rotating mechanism installed inside the shell to improve the stirring effect of the polypeptide, a stirring mechanism installed inside the shell for improving the elution effect of the polypeptide, the rotating mechanism comprising a rotating column one, the rotating column one being installed on the inner bottom of the shell, a gear one being fixedly connected to the top of the rotating column one, a gear two being meshed with the right side of the gear one, a cylinder being fixedly connected to the top of the gear one, a rotating column two being fixedly connected to the middle of the gear two, and a drive assembly being installed at the right end of the shell.

[0006] As a further description of the above technical solution:

[0007] The drive assembly includes a base, which is located at the bottom right side of the housing. A motor is fixedly connected to the top of the base. A rotating shaft is fixedly connected to the output end of the motor. A belt pulley transmission mechanism is fixedly connected to the outside of the rotating shaft. A rotating column is fixedly connected to the left side of the belt pulley transmission mechanism.

[0008] As a further description of the above technical solution:

[0009] The stirring mechanism includes fixed rods, and multiple fixed rods are respectively installed around the inside of the housing. Scrapers are fixedly connected to the outer perimeter of each fixed rod, and stirring columns are fixedly connected to the inner perimeter of each fixed rod. A filter plate is installed on the upper outer part of the stirring column, and sliders are fixedly connected to the outer perimeter of each filter plate. A sliding groove is slidably connected to the outer side of the slider. An actuation component is installed inside the housing.

[0010] As a further description of the above technical solution:

[0011] The execution component includes a second motor, which is installed at the top center of the housing. A transmission rod is fixedly connected to the output end of the second motor, and a rotating rod is fixedly connected to the bottom end of the transmission rod. The rotating rod is fixedly connected to the stirring column.

[0012] As a further description of the above technical solution:

[0013] A feed inlet is installed on the top front side of the outer shell, and support columns are fixedly connected to all four sides of the outer wall of the outer shell.

[0014] As a further description of the above technical solution:

[0015] A discharge port is provided on the bottom left side of the outer casing, and a filter box is fixedly connected to the bottom of the outer casing.

[0016] As a further description of the above technical solution:

[0017] The filter box has a filter plate two fixedly connected inside, and the filter box has a discharge port on the bottom left side.

[0018] As a further description of the above technical solution:

[0019] A collection box is provided at the bottom of the discharge port, and handles are installed on the left and right sides of the top of the collection box.

[0020] This utility model has the following beneficial effects:

[0021] In this invention, the starting motor drives the belt pulley transmission mechanism to rotate, which in turn drives the rotating column to rotate. The rotating column rotates with the gear, and the gear meshes with the gear, transmitting power to the rotating column. The rotating column drives the cylinder to rotate, and the peptide to be eluted is fed into the cylinder from the feed inlet. The rotation of the cylinder ensures that the substance is fully mixed with the eluent, and the elution efficiency is improved through mechanical stirring.

[0022] 2. In this utility model, the starting motor drives the transmission rod to rotate, which in turn drives the rotating rod to rotate. The stirring column fixed on the rotating rod rotates in the opposite direction to the cylinder, improving the elution efficiency. The fixed rod on the rotating rod is equipped with a scraper to scrape off residual peptides so that they can re-participate in the reaction. The trough and the slider cooperate to filter larger impurities during feeding. The filter plate can be quickly disassembled and installed by sliding the slider in the trough, which is convenient for cleaning and replacement to ensure equipment efficiency. Attached Figure Description

[0023] Figure 1 This is a front view of a polypeptide elution device proposed in this utility model;

[0024] Figure 2 This is a perspective view of a polypeptide elution device proposed in this utility model;

[0025] Figure 3 This is a partial structural exploded view of a polypeptide elution device proposed in this utility model;

[0026] Figure 4 This is a partial structural schematic diagram of a polypeptide elution device proposed in this utility model;

[0027] Figure 5 This is a partial exploded view of the structure of a polypeptide elution device proposed in this utility model.

[0028] Legend:

[0029] 1. Outer shell; 2. Rotating mechanism; 201. Rotating column one; 202. Gear one; 203. Cylinder; 204. Gear two; 205. Rotating column two; 206. Drive assembly; 2061. Base; 2062. Motor one; 2063. Rotating shaft; 2064. Belt pulley transmission mechanism; 3. Stirring mechanism; 301. Fixed rod; 302. Scraper; 303. Stirring column; 304. Slider; 305. Slide groove; 306. Filter plate one; 307. Actuating assembly; 3071. Motor two; 3072. Transmission rod; 3073. Rotating rod; 4. Feed inlet; 5. Support column; 6. Discharge outlet; 7. Handle; 8. Collection box; 9. Filter box; 10. Filter plate two; 11. Discharge port. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] Reference Figure 3 and Figure 4 This utility model provides an embodiment of a polypeptide elution device, comprising a housing 1, a rotating mechanism 2 installed inside the housing 1 to improve the stirring effect of the polypeptide, and a stirring mechanism 3 installed inside the housing 1 to improve the elution effect of the polypeptide. The rotating mechanism 2 includes a rotating column 201 installed at the bottom inner side of the housing 1, a gear 202 fixedly connected to the top of the rotating column 201, a gear 204 meshing with the right side of the gear 202, a cylinder 203 fixedly connected to the top of the gear 202, and a cylinder 203 fixedly connected to the middle of the gear 204. A rotating column 205 is fixedly connected. A drive assembly 206 is installed at the right end of the outer casing 1. The drive assembly 206 includes a base 2061. The base 2061 is located at the bottom right side of the outer casing 1. A motor 2062 is fixedly connected to the top of the base 2061. A rotating shaft 2063 is fixedly connected to the output end of the motor 2062. A belt pulley transmission mechanism 2064 is fixedly connected to the outside of the rotating shaft 2063. A rotating column 205 is fixedly connected to the left side of the belt pulley transmission mechanism 2064. A discharge port 11 is opened on the bottom left side of the outer casing 1. A filter box 9 is fixedly connected to the bottom of the outer casing 1.

[0032] Specifically, during peptide elution, motor 2062 is started, driving belt drive mechanism 2064 to rotate. Belt drive mechanism 2064 drives rotating column 205 to rotate. Rotating column 205 is connected to gear 204, which meshes with gear 202, transmitting power to rotating column 201. Rotating column 201 is connected to cylinder 203. When rotating column 201 rotates, it drives cylinder 203 to rotate, displacing the peptides to be eluted from... The feed inlet 4 is inserted into the cylinder 203. The rotation of the cylinder 203 makes the material and the eluent fully mixed, thereby improving the elution effect. After elution, the material flows into the filter box 9 through the discharge port 11. The filter plate 10 in the filter box 9 can intercept impurity particles with a diameter larger than the mesh pore size, realizing the precision filtration of the eluted product, thereby extracting higher quality peptides. The filtered product flows into the collection box 8 through the discharge port 6. The handle 7 installed on the outside of the collection box 8 makes it easy for staff to move and transfer the collection box 8.

[0033] Reference Figure 2 and Figure 5 The stirring mechanism 3 includes a fixed rod 301, and multiple fixed rods 301 are respectively installed around the inside of the outer shell 1. Scrapers 302 are fixedly connected to the outer sides of the fixed rods 301, and stirring columns 303 are fixedly connected to the inner sides of the fixed rods 301. A filter plate 306 is installed on the upper middle part of the outer side of the stirring column 303. A slider 304 is fixedly connected to the outer sides of the filter plate 306. A sliding groove 305 is slidably connected to the outer side of the slider 304. An execution component 307 is installed inside the outer shell 1. The execution component 307 includes a second motor 3071. The second motor 3071 is installed at the top middle of the outer shell 1. A transmission rod 3072 is fixedly connected to the output end of the second motor 3071. A rotating rod 3073 is fixedly connected to the bottom end of the transmission rod 3072. The rotating rod 3073 is fixedly connected to the stirring column 303. A second filter plate 10 is fixedly connected inside the filter box 9. A discharge port 6 is opened on the bottom left side of the filter box 9.

[0034] Specifically, starting motor 3071 drives transmission rod 3072 to rotate, which in turn drives rotating rod 3073 to rotate. The stirring column 303 mounted on rotating rod 3073 rotates in the opposite direction to cylinder 203, further improving elution efficiency. There is also a fixed rod 301 on rotating rod 3073. The scraper 302 mounted at the end of fixed rod 301 rotates with rotating rod 3073 and maintains a precise fit with the inner wall of cylinder 203. This effectively scrapes away residual peptides attached to the inner wall of cylinder 203, allowing these peptides to re-participate in the elution reaction and improving peptide recovery rate. The groove 305 on the inner wall of cylinder 203 is connected to filter plate 306. During the feeding stage, filter plate 306 performs preliminary filtration of the input material, intercepting larger impurity particles. By sliding slider 304 within groove 305, filter plate 306 can be quickly disassembled and installed, facilitating cleaning or replacement of filter plate 306 during equipment maintenance and ensuring continuous and stable operation of the equipment.

[0035] Reference Figure 1 The top front side of the outer shell 1 is equipped with a feed inlet 4, and the outer walls of the outer shell 1 are all fixedly connected with support columns 5. The bottom of the discharge port 6 is equipped with a collection box 8, and the top left and right sides of the collection box 8 are equipped with handles 7.

[0036] Specifically, the outer shell 1 has a feed inlet 4 at the top, support columns 5 fixed around it, and a collection box 8 below the discharge outlet 6. The collection box 8 is equipped with handles 7 on the left and right sides.

[0037] Working principle: During peptide elution, motor 2062 is started, driving belt drive mechanism 2064 to rotate. Belt drive mechanism 2064 drives rotating column 205 to rotate. Rotating column 205 rotates synchronously with gear 204. Gear 204 meshes with gear 202, transmitting power to rotating column 201. Rotating column 201 is connected to cylinder 203. When rotating column 201 rotates, it drives cylinder 203 to rotate, feeding the peptides to be eluted through inlet 4. The material is fed into the cylinder 203. The rotation of the cylinder 203 thoroughly mixes the material with the eluent. The mechanical stirring action improves the elution efficiency. After elution, the material flows into the filter box 9 through the discharge port 11. The filter plate 10 in the filter box 9 can intercept impurity particles with a diameter larger than the mesh pore size, achieving precise filtration of the eluted product, thereby extracting higher quality peptides. The filtered product flows into the collection box 8 through the discharge port 6. The handle 7 installed on the outside of the collection box 8 facilitates the handling and transfer of the collection box 8 by the staff.

[0038] Motor 3071 is started, driving transmission rod 3072 to rotate. Transmission rod 3072 drives rotating rod 3073 to rotate. The stirring column 303 fixedly installed on rotating rod 3073 rotates in the opposite direction to cylinder 203, further improving elution efficiency. A fixing rod 301 is also fixed on rotating rod 3073. The scraper 302 installed on fixing rod 301 rotates with rotating rod 3073. Its edge maintains a small gap with the inner wall of cylinder 203, which can scrape off residual waste adhering to the inner wall of cylinder 203. Peptides are added to allow these polypeptides to re-participate in the elution reaction, improving the polypeptide recovery rate. The inner wall of the cylinder 203 has a groove 305, and the outer edge of the filter plate 306 is equipped with a matching slider 304. During the feeding stage, the filter plate 306 performs preliminary filtration on the input material, intercepting larger impurity particles. By sliding the slider 304 in the groove 305, the filter plate 306 can be quickly disassembled and installed, facilitating cleaning and replacement of the filter plate 306 and ensuring the continuous operating efficiency of the equipment.

[0039] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A polypeptide elution apparatus, comprising a housing (1), characterized in that: The shell (1) is equipped with a rotating mechanism (2) to improve the stirring effect of the polypeptide. The shell (1) is also equipped with a stirring mechanism (3) to improve the elution effect of the polypeptide. The rotating mechanism (2) includes a rotating column (201), which is installed on the bottom inner side of the outer casing (1). A gear (202) is fixedly connected to the top of the rotating column (201), and a gear (204) is meshed with the right side of the gear (202). A cylinder (203) is fixedly connected to the top of the gear (202), and a rotating column (205) is fixedly connected to the middle of the gear (204). A drive assembly (206) is installed at the right end of the outer casing (1).

2. The polypeptide elution apparatus according to claim 1, characterized in that: The drive assembly (206) includes a base (2061), which is located at the bottom right side of the outer casing (1). A motor (2062) is fixedly connected to the top of the base (2061). A rotating shaft (2063) is fixedly connected to the output end of the motor (2062). A belt pulley transmission mechanism (2064) is fixedly connected to the outside of the rotating shaft (2063). The rotating column (205) is fixedly connected to the left side of the belt pulley transmission mechanism (2064).

3. The polypeptide elution apparatus according to claim 1, characterized in that: The stirring mechanism (3) includes a fixed rod (301), and multiple fixed rods (301) are respectively installed around the inside of the outer shell (1). Scrapers (302) are fixedly connected to the outer periphery of the fixed rods (301), and stirring columns (303) are fixedly connected to the inner periphery of the fixed rods (301). A filter plate (306) is installed on the upper middle part of the outer periphery of the stirring column (303). A slider (304) is fixedly connected to the outer periphery of the filter plate (306). A sliding groove (305) is slidably connected to the outer periphery of the slider (304). An actuator (307) is installed inside the outer shell (1).

4. The polypeptide elution apparatus according to claim 3, characterized in that: The execution component (307) includes a second motor (3071), which is installed at the top center of the outer casing (1). The output end of the second motor (3071) is fixedly connected to a transmission rod (3072), and the bottom end of the transmission rod (3072) is fixedly connected to a rotating rod (3073). The rotating rod (3073) is fixedly connected to the stirring column (303).

5. The polypeptide elution apparatus according to claim 1, characterized in that: The top front side of the outer shell (1) is equipped with a feed port (4), and the outer walls of the outer shell (1) are all fixedly connected with support columns (5).

6. The polypeptide elution apparatus according to claim 1, characterized in that: The bottom left side of the outer shell (1) is provided with a discharge port (11), and a filter box (9) is fixedly connected to the bottom of the outer shell (1).

7. The polypeptide elution apparatus according to claim 6, characterized in that: The filter box (9) is fixedly connected to the filter plate 2 (10), and the filter box (9) has a discharge port (6) on the bottom left side.

8. The polypeptide elution apparatus according to claim 7, characterized in that: A collection box (8) is provided at the bottom of the discharge port (6), and handles (7) are installed on the left and right sides of the top of the collection box (8).

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