A multi-stage filtration system for preparing ergothioneine

Abstract

The utility model relates to a kind of multistage filtration systems for preparing ergothioneine, including fermentation tank, first feed pump, coarse filter, ceramic membrane filtration assembly, ultrafiltration membrane filtration assembly, buffer tank and second feed pump, the input end of first feed pump is connected with the outlet of fermentation tank, the output end of first feed pump is connected with the import of coarse filter by second pipeline, the ceramic membrane filtration assembly includes multiple ceramic membrane filters, the import of multiple ceramic membrane filters is respectively communicated with the outlet of coarse filter by third pipeline, the dialysate outlet of multiple ceramic membrane filters is respectively communicated with the import of ultrafiltration membrane filtration assembly by fourth pipeline, the concentrated liquid outlet of multiple ceramic membrane filters is respectively communicated with the import of buffer tank by fifth pipeline, the input end of second feed pump is connected with the outlet of buffer tank by sixth pipeline, the output end of second feed pump is communicated with second pipeline.The utility model can improve the fermentation broth filtration efficiency and ergothioneine recovery rate.

Description

Technical Field

[0001] This utility model relates to the field of ergothioneine preparation technology, specifically to a multi-stage filtration system for preparing ergothioneine. Background Technology

[0002] Ergothioneine is a natural antioxidant, a natural amino acid derived from plants and capable of accumulating in animals. It possesses strong antioxidant activity, capable of scavenging reactive oxygen species, chelating divalent metal ions, activating antioxidant enzymes, inhibiting superoxide dismutase, and suppressing the oxidation of various heme proteins. The extraction process for ergothioneine via bio-fermentation synthesis requires a filtration system to remove large molecular impurities such as particles, proteins, polysaccharides, and pigments. However, existing filtration systems are slow to filter fermentation broth and have low ergothioneine recovery rates. Utility Model Content

[0003] This invention addresses the shortcomings of existing technologies by providing a multi-stage filtration system for preparing ergothioneine, thereby improving filtration efficiency and ergothioneine recovery rate.

[0004] This utility model is achieved through the following technical solution: a multi-stage filtration system for preparing ergothioneine, comprising a fermenter, a first feed pump, a coarse filter, a ceramic membrane filter assembly, an ultrafiltration membrane filter assembly, a buffer tank, and a second feed pump. The outlet of the fermenter is connected to the input end of the first feed pump via a first pipeline, and the output end of the first feed pump is connected to the inlet of the coarse filter via a second pipeline. The ceramic membrane filter assembly includes multiple ceramic membrane filters, the inlets of which are respectively connected to the outlet of the coarse filter via a third pipeline, the dialysate outlets of which are respectively connected to the inlet of the ultrafiltration membrane filter assembly via a fourth pipeline, the concentrate outlets of which are respectively connected to the inlet of the buffer tank via a fifth pipeline, the outlet of the buffer tank is connected to the input end of the second feed pump via a sixth pipeline, and the output end of the second feed pump is connected to the second pipeline via a seventh pipeline.

[0005] This method involves fermenting ergothioneine-containing broth in a fermenter. The broth is then pumped to a coarse filter to remove large particles. Next, it enters a ceramic membrane filter assembly for primary fine filtration, removing particulate impurities. This ceramic membrane filter assembly, with multiple ceramic membrane filters connected in parallel with the coarse filter, allows for the diversion of the fermentation broth for separate filtration. This diversion reduces fluid pressure and flow rate, thereby improving overall filtration efficiency. The concentrate obtained from the ceramic membrane filter is stored in a buffer tank. Since the concentrate typically contains a small amount of unprecipitated ergothioneine, it is pumped back to the coarse filter via a second pump. Multiple cycles of filtration through the coarse filter and ceramic membrane filter assembly ensure complete ergothioneine precipitation, improving the ergothioneine recovery rate. Finally, the dialysate undergoes secondary fine filtration through an ultrafiltration membrane assembly to remove molecular impurities, yielding high-purity ergothioneine.

[0006] As an optimization, the coarse filter is a security filter. This optimized security filter offers high filtration accuracy, more uniform filtration of large particulate impurities, and no contamination of the filter media.

[0007] As an optimization, a first electrically controlled valve is installed on multiple of the third pipelines. This optimized solution facilitates the control of the opening and closing of the inlets of each ceramic membrane filter.

[0008] As an optimization, a second electrically controlled valve is installed on multiple of the fifth pipelines. This optimized design facilitates the control of the opening and closing of the concentrate outlet of each ceramic membrane filter.

[0009] As an optimization, a first three-way reversing valve is installed on the second pipeline. The three ports of the first three-way reversing valve are respectively connected to the output end of the first feed pump, the seventh pipeline, and the inlet of the coarse filter. This optimized solution, through the reversing function of the first three-way reversing valve, can control the connection between the output end of the first feed pump and the inlet of the coarse filter, or between the seventh pipeline and the inlet of the coarse filter, making it more convenient to use.

[0010] As an optimization, the outlet of the buffer tank is also connected to a drain pipe, and the outlet of the buffer tank, the drain pipe, and the sixth pipeline are connected via a second three-way reversing valve. This optimized solution, through the reversing function of the second three-way reversing valve, can control the connection between the outlet of the buffer tank and the sixth pipeline, or between the outlet of the buffer tank and the drain pipe, making it more convenient to use.

[0011] As an optimization, a cooling system is also included. This system comprises cooling coils and a cooling tower. The cooling coils are fixed to the outer wall of the buffer tank. The outlet of the cooling coils is connected to the inlet of the cooling tower via a first circulation pipeline, and the outlet of the cooling tower is connected to the inlet of the cooling coils via a second circulation pipeline. A circulation pump and a third electrically controlled valve are installed on the second circulation pipeline. This optimized solution uses the cooling tower to provide cooling water to cool the buffer tank, ensuring the concentrate remains at a low temperature, which improves the precipitation effect of ergothioneine and increases the recovery rate.

[0012] As an optimization, a temperature sensor is installed on the buffer tank. This optimization facilitates temperature detection and control.

[0013] As an optimization, the ultrafiltration membrane filtration assembly includes a primary ultrafiltration membrane filter and a secondary ultrafiltration membrane filter, which are connected in series. The fourth pipeline is connected to the inlet of the primary ultrafiltration membrane filter, and the outlet of the primary ultrafiltration membrane filter is connected to the inlet of the secondary ultrafiltration membrane filter. A collection tank is connected to the outlet of the secondary ultrafiltration membrane filter. This optimized solution ensures effective filtration of molecular impurities through multi-stage filtration using two ultrafiltration membrane filters.

[0014] As an optimization, the molecular weight cutoff of the primary ultrafiltration membrane filter is greater than that of the secondary ultrafiltration membrane filter. The primary ultrafiltration membrane filter can remove large molecular impurities such as proteins and polysaccharides, while the secondary ultrafiltration membrane filter further removes small molecular impurities such as pigments.

[0015] The beneficial effects of this invention are as follows: the fermentation broth is filtered through a multi-stage filter consisting of a coarse filter, a ceramic membrane filter assembly, and an ultrafiltration membrane filter assembly, thereby achieving multi-stage filtration of large particulate impurities, microparticle impurities, and molecular impurities, and obtaining high-purity ergothioneine.

[0016] The ceramic membrane filtration assembly, by setting up multiple ceramic membrane filters in parallel with a coarse filter, can divert the delivered fermentation broth for separate filtration. This diversion reduces fluid pressure and individual filter flow rates, thereby improving overall filtration effect and efficiency. The concentrated liquid obtained after passing through the ceramic membrane filter enters a buffer tank for storage. A second feed pump can then circulate the concentrated liquid, allowing it to undergo multiple cycles of filtration through the coarse filter and ceramic membrane filtration assembly, ensuring complete ergothioneine extraction and improving ergothioneine recovery rate.

[0017] The ultrafiltration membrane filtration unit uses a two-stage ultrafiltration membrane filter to filter the dialysate in multiple stages, ensuring the filtration effect on molecular impurities. By filtering out molecular impurities, high-purity ergothioneine can be obtained. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the process of this utility model;

[0019] As shown in the figure:

[0020] 1. Fermentation tank; 2. First feed pump; 3. Coarse filter; 4. Ceramic membrane filter assembly; 41. Ceramic membrane filter; 5. Ultrafiltration membrane filter assembly; 51. Primary ultrafiltration membrane filter; 52. Secondary ultrafiltration membrane filter; 6. Buffer tank; 7. Second feed pump; 8. Collection tank; 9. Cooling coil; 10. Cooling tower; 11. First pipeline; 12. Second pipeline; 13. First three-way reversing valve; 14. Eighth pipeline; 15. Third pipeline; 16. First solenoid valve; 17. Fourth pipeline; 18. Ninth pipeline; 19. Fifth pipeline; 20. Second solenoid valve; 21. Tenth pipeline; 22. Temperature sensor; 23. First circulation pipeline; 24. Second circulation pipeline; 25. Circulation pump; 26. Third solenoid valve; 27. Second three-way reversing valve; 28. Sixth pipeline; 29. ​​Seventh pipeline; 30. Drain pipe. Detailed Implementation

[0021] To clearly illustrate the technical features of this solution, the following detailed implementation method will be used to explain the solution.

[0022] like Figure 1 As shown, a multi-stage filtration system for preparing ergothionein includes a fermenter 1, a first feed pump 2, a coarse filter 3, a ceramic membrane filter assembly 4, an ultrafiltration membrane filter assembly 5, a buffer tank 6, and a second feed pump 7.

[0023] The outlet of the fermenter 1 is connected to the input of the first feed pump 2 via a first pipeline 11, and the output of the first feed pump 2 is connected to the inlet of the coarse filter 3 via a second pipeline 12. In this embodiment, the coarse filter 3 is a security filter. Fermentation in the fermenter 1 yields a fermentation broth containing ergothioneine. This broth is then pumped to the coarse filter 3 via the first feed pump 2 for preliminary coarse filtration, removing large particulate impurities. The security filter, however, provides high filtration precision, filters large particulate impurities more uniformly, and does not contaminate the filter medium.

[0024] The ceramic membrane filtration assembly 4 includes multiple ceramic membrane filters 41. In this embodiment, the ceramic membrane filtration assembly 4 includes three ceramic membrane filters 41, and the ceramic membrane filters 41 use 50 nm ceramic membranes. The ceramic membrane filters 41 are existing technology and have an inlet, a concentrate outlet, and a dialysate outlet. After the fermentation broth is filtered through the ceramic membrane, ergot sulfide components can pass through the ceramic membrane to form dialysate, while the liquid blocked by the ceramic membrane forms the concentrate.

[0025] The inlets of multiple ceramic membrane filters 41 are connected to the outlet of the coarse filter 3 via third pipes 15. Each of the third pipes 15 is equipped with a first electrically controlled valve 16 to facilitate the opening and closing of the inlets of each ceramic membrane filter. In this embodiment, the outlet of the coarse filter 3 is connected to an eighth pipe 14, and the three ceramic membrane filters 41 are connected in parallel to the eighth pipe 14 via three third pipes 15.

[0026] The dialysate outlets of multiple ceramic membrane filters 41 are respectively connected to the inlet of the ultrafiltration membrane filter assembly 5 through the fourth pipe 17. In this embodiment, the inlet of the ultrafiltration membrane filter assembly 5 is connected to the ninth pipe 18, and the three ceramic membrane filters 41 are connected in parallel to the ninth pipe 18 through three fourth pipes 17.

[0027] The concentrate outlets of multiple ceramic membrane filters 41 are connected to the inlet of buffer tank 6 via fifth pipes 19. Each of the fifth pipes 19 is equipped with a second electrically controlled valve 20 to facilitate control of the opening and closing of the concentrate outlets of each ceramic membrane filter. In this embodiment, the inlet of buffer tank 6 is connected to a tenth pipe 21, and the three ceramic membrane filters 41 are connected in parallel to the tenth pipe 21 via three fifth pipes 19.

[0028] The outlet of the buffer tank 6 is connected to the input end of the second feed pump 7 through the sixth pipeline 28, and the output end of the second feed pump 7 is connected to the second pipeline 12 through the seventh pipeline 29.

[0029] In this embodiment, a first three-way reversing valve 13 is installed on the second pipeline 12. The three ports of the first three-way reversing valve 13 are respectively connected to the output end of the first feed pump 2, the seventh pipeline 29, and the inlet of the coarse filter 3. By using the reversing function of the first three-way reversing valve 13, the output end of the first feed pump 2 can be connected to the inlet of the coarse filter 3, or the seventh pipeline 29 can be connected to the inlet of the coarse filter 3, making it more convenient to use.

[0030] The fermentation broth, after coarse filtration, undergoes primary fine filtration through ceramic membrane filter assembly 4 to remove particulate impurities. Ceramic membrane filter assembly 4, by setting multiple ceramic membrane filters 41 in parallel with the coarse filter 3, allows the fermentation broth to be diverted for separate filtration. This diversion reduces fluid pressure and the filtration flow rate of individual ceramic membrane filters, thereby improving the overall filtration effect and efficiency. The concentrated broth obtained after filtration enters buffer tank 6 for buffer storage. Typically, the concentrated broth contains a small amount of unprecipitated ergothioneine. The second feed pump 7 can return the concentrated broth to the second pipeline 12, where it undergoes multiple cycles of filtration through the coarse filter 3 and ceramic membrane filter assembly 4 to ensure complete ergothioneine precipitation, thus improving the ergothioneine recovery rate. The dialysate obtained after filtration then undergoes secondary fine filtration through ultrafiltration membrane filter assembly 5 to remove molecular impurities, yielding high-purity ergothioneine.

[0031] The outlet of the buffer tank 6 is also connected to a drain pipe 30. The outlet of the buffer tank 6, the drain pipe 30, and the sixth pipeline 28 are connected via a second three-way reversing valve 27. By using the reversing function of the second three-way reversing valve 27, when it is necessary to circulate and filter the concentrate, the outlet of the buffer tank 6 can be connected to the sixth pipeline 28. When it is not necessary to circulate and filter the concentrate, the outlet of the buffer tank 6 can be connected to the drain pipe 30, and the concentrate can be discharged through the drain pipe for collection and treatment.

[0032] The system also includes a cooling system comprising a cooling coil 9 and a cooling tower 10. The cooling coil 9 is fixed to the outer wall of the buffer tank 6. The outlet of the cooling coil 9 is connected to the inlet of the cooling tower 10 via a first circulation pipe 23, and the outlet of the cooling tower 10 is connected to the inlet of the cooling coil 9 via a second circulation pipe 24. A circulation pump 25 and a third electrically controlled valve 26 are installed on the second circulation pipe 24. Cooling water supplied by the cooling tower 10 cools the buffer tank 6, ensuring the concentrate is kept at a low temperature, which improves the precipitation effect of ergothioneine and increases the recovery rate of ergothioneine.

[0033] The buffer tank 6 is equipped with a temperature sensor 22 for convenient temperature detection and control.

[0034] Specifically, the ultrafiltration membrane filtration assembly 5 includes a primary ultrafiltration membrane filter 51 and a secondary ultrafiltration membrane filter 52. The primary ultrafiltration membrane filter 51 and the secondary ultrafiltration membrane filter 52 are connected in series, and the fourth pipeline 17 is connected to the inlet of the primary ultrafiltration membrane filter 51. The outlet of the primary ultrafiltration membrane filter 51 is connected to the inlet of the secondary ultrafiltration membrane filter 52, and the outlet of the secondary ultrafiltration membrane filter 52 is connected to a collection tank 8.

[0035] In this embodiment, the ninth pipeline 18 is connected to the inlet of the first-stage ultrafiltration membrane filter 51. When three fourth pipelines 17 are connected in parallel on the ninth pipeline 18, they are connected to the inlet of the first-stage ultrafiltration membrane filter 51.

[0036] The molecular weight cutoff of the primary ultrafiltration membrane filter 51 is greater than that of the secondary ultrafiltration membrane filter 52. In this embodiment, the molecular weight cutoff of the primary ultrafiltration membrane filter 51 is 3000 Da, and the molecular weight cutoff of the secondary ultrafiltration membrane filter 52 is 1000 Da. The primary ultrafiltration membrane filter removes large molecular impurities such as proteins and polysaccharides, while the secondary ultrafiltration membrane filter further removes small molecular impurities such as pigments. Through multi-stage filtration using two ultrafiltration membrane filters, the filtration effect on molecular impurities is ensured, resulting in high-purity ergothioneine, which is stored in the collection tank 8.

[0037] Working principle: Fermentation broth containing ergothionein is obtained through fermentation in fermenter 1. At this time, the first three-way reversing valve 13 controls the output end of the first feed pump 2 to connect with the inlet of the coarse filter 3. The fermentation broth is transported to the coarse filter 3 through the first feed pump 2 for preliminary coarse filtration to remove large particulate impurities.

[0038] The fermentation broth, after initial coarse filtration, then enters the ceramic membrane filter assembly 4 for primary fine filtration to remove particulate impurities. The ceramic membrane filter assembly 4, by setting multiple ceramic membrane filters 41 connected in parallel with the coarse filter 3, can split the fermentation broth for separate filtration, improving the overall filtration effect and efficiency. The concentrated solution obtained through the ceramic membrane filter 41 enters the buffer tank 6 for temporary buffering, while the dialysate undergoes secondary fine filtration through the ultrafiltration membrane filter assembly 5 to remove molecular impurities, thus obtaining high-purity ergothioneine, which is stored in the collection tank 8.

[0039] The concentrate typically contains a small amount of unprecipitated ergothioneine. Therefore, the first three-way reversing valve 13 connects the seventh pipeline 29 to the inlet of the coarse filter 3, and the second three-way reversing valve 27 connects the outlet of the buffer tank 6 to the sixth pipeline 28. The concentrate is then pumped back to the coarse filter 3 via the second feed pump 7. After multiple cycles of filtration through the coarse filter 3 and the ceramic membrane filter assembly 4, the ergothioneine in the concentrate is completely precipitated, improving the ergothioneine recovery rate.

[0040] After the concentrate has been filtered through multiple cycles, the second three-way reversing valve 27 connects the outlet of the buffer tank 6 to the drain pipe 30, discharging the concentrate for collection and treatment.

[0041] Of course, the above description is not limited to the examples above. Technical features of this utility model not described can be implemented by or using existing technology, and will not be repeated here. The above embodiments and drawings are only used to illustrate the technical solution of this utility model and are not intended to limit this utility model. This utility model has been described in detail with reference to preferred embodiments. Those skilled in the art should understand that any changes, modifications, additions or substitutions made by those skilled in the art within the scope of this utility model do not depart from the spirit of this utility model and should also fall within the protection scope of the claims of this utility model.

Claims

1. A multi-stage filtration system for preparing ergothioneine, characterized in that: It includes a fermenter (1), a first feed pump (2), a coarse filter (3), a ceramic membrane filter assembly (4), an ultrafiltration membrane filter assembly (5), a buffer tank (6), and a second feed pump (7). The outlet of the fermenter (1) is connected to the input end of the first feed pump (2) through a first pipeline (11), and the output end of the first feed pump (2) is connected to the inlet of the coarse filter (3) through a second pipeline (12). The ceramic membrane filtration assembly (4) includes multiple ceramic membrane filters (41). The inlets of the multiple ceramic membrane filters (41) are connected to the outlet of the coarse filter (3) through the third pipeline (15). The dialysate outlets of the multiple ceramic membrane filters (41) are connected to the inlet of the ultrafiltration membrane filtration assembly (5) through the fourth pipeline (17). The concentrate outlets of the multiple ceramic membrane filters (41) are connected to the inlet of the buffer tank (6) through the fifth pipeline (19). The outlet of the buffer tank (6) is connected to the input end of the second feed pump (7) through the sixth pipeline (28). The output end of the second feed pump (7) is connected to the second pipeline (12) through the seventh pipeline (29).

2. The multi-stage filtration system for preparing ergothioneine according to claim 1, characterized in that: The coarse filter (3) is a security filter.

3. The multi-stage filtration system for preparing ergothioneine according to claim 1, characterized in that: Each of the third pipelines (15) is equipped with a first electrically controlled valve (16).

4. The multi-stage filtration system for preparing ergothioneine according to claim 1, characterized in that: A second electrically controlled valve (20) is installed on each of the fifth pipelines (19).

5. The multi-stage filtration system for preparing ergothioneine according to claim 1, characterized in that: The second pipeline (12) is equipped with a first three-way reversing valve (13), and the three ports of the first three-way reversing valve (13) are respectively connected to the output end of the first feed pump (2), the seventh pipeline (29) and the inlet of the coarse filter (3).

6. The multi-stage filtration system for preparing ergothioneine according to claim 1, characterized in that: The outlet of the buffer tank (6) is also connected to a drain pipe (30), and the outlet of the buffer tank (6), the drain pipe (30) and the sixth pipeline (28) are connected by a second three-way reversing valve (27).

7. The multi-stage filtration system for preparing ergothioneine according to claim 1, characterized in that: It also includes a cooling system, which includes a cooling coil (9) and a cooling tower (10). The cooling coil (9) is fixed to the outer wall of the buffer tank (6). The outlet of the cooling coil (9) is connected to the inlet of the cooling tower (10) through the first circulation pipe (23). The outlet of the cooling tower (10) is connected to the inlet of the cooling coil (9) through the second circulation pipe (24). A circulation pump (25) and a third electrically controlled valve (26) are installed on the second circulation pipe (24).

8. The multi-stage filtration system for preparing ergothioneine according to claim 7, characterized in that: A temperature sensor (22) is installed on the buffer tank (6).

9. The multi-stage filtration system for preparing ergothioneine according to any one of claims 1 to 8, characterized in that: The ultrafiltration membrane filtration assembly (5) includes a primary ultrafiltration membrane filter (51) and a secondary ultrafiltration membrane filter (52), which are connected in series. The fourth pipeline (17) is connected to the inlet of the primary ultrafiltration membrane filter (51), the outlet of the primary ultrafiltration membrane filter is connected to the inlet of the secondary ultrafiltration membrane filter, and the outlet of the secondary ultrafiltration membrane filter (52) is connected to a collection tank (8).

10. The multi-stage filtration system for preparing ergothioneine according to claim 9, characterized in that: The molecular weight cutoff of the primary ultrafiltration membrane filter (51) is greater than that of the secondary ultrafiltration membrane filter (52).

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