Triple spray film instrument for producing animal epidemic disease triple detection card

Abstract

The utility model discloses a kind of triple membrane spraying instruments for animal epidemic disease triple detection card production, it is related to detection card membrane scriber field.The utility model rigid rack, closed frame formed by base, top plate and side wall;High-precision plane displacement platform, integrated in base, include: X-axis linear sliding table, by first servo motor through ball screw drive, the utility model is through three piezoelectric micro-nozzle in the rigidity isolation layout of Y-axis direction, thoroughly eliminate the cross-contamination of different biological reagent from physical structure, nozzle is preset interval synchronous spraying, ensure hog cholera, foot-and-mouth disease etc. Detection line is independently formed on nitrocellulose membrane, thereby produce test paper as shown in figure 4, wherein three groups of detection line are in ladder structure, further avoid the problem that when detection liquid flows to second, third detection line, its concentration and effective combination ability have dropped, reduce the probability of false negative appearance.

Description

Technical Field

[0001] This utility model relates to the field of testing card coating instruments, specifically a triple coating instrument for producing animal disease triple testing cards. Background Technology

[0002] The test card scribing instrument uses a sophisticated mechanical control system to drive a nozzle or scribing needle loaded with a solution of a specific biological reagent (such as an antibody or antigen) to precisely scribble and spray a small amount of liquid onto a nitrocellulose membrane (NC membrane) or other chromatographic membrane substrate at a preset path, speed, and width.

[0003] Currently, multiplex coating instruments on the market require a relatively large amount of solution to cover the entire linewidth. Several coating antigens or antibodies may overlap in space, potentially reducing the sensitivity of downstream detection lines. Furthermore, traditional coating needles can damage the membrane structure and alter chromatography performance. Therefore, the inventors urgently need to design a coating instrument capable of step-by-step spraying to reduce the probability of false negatives. Utility Model Content

[0004] Based on this, the purpose of this utility model is to provide a triple spraying instrument for the production of animal disease triple test cards, so as to solve the technical problem that the antigens or antibodies applied by the traditional spraying instrument have "crossing" in space, which may reduce the sensitivity of downstream detection lines.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a triple-spray film machine for producing animal disease triple test cards, comprising: a rigid frame, and a closed frame consisting of a base, a top plate, and side walls;

[0006] A high-precision planar displacement platform, integrated into the base, includes:

[0007] The X-axis linear slide is driven by the first servo motor via a ball screw.

[0008] The Y-axis linear slide is orthogonally arranged above the X-axis slide and is driven by the second servo motor.

[0009] The membrane material support platform, fixed to the Y-axis slide, is used to adsorb and fix the nitrocellulose membrane;

[0010] The Z-axis lifting mechanism is suspended below the top plate and is driven by a linear motor to achieve vertical movement via a precision guide rail.

[0011] A micro-fluid distribution unit, connected to the end of the Z-axis lifting mechanism, includes:

[0012] Three independent piezoelectric micro-nozzles are rigidly arranged at a preset spacing in the Y-axis direction, and each nozzle is connected to a liquid pump through an independent microchannel.

[0013] Stainless steel cutting blades are fixed to the rear of the nozzle bracket along the negative Y-axis.

[0014] The control system is configured to synchronously drive the displacement platform, nozzle start / stop, and cutter action.

[0015] By adopting the above technical solution, a stable closed frame is constructed through a rigid frame to ensure the mechanical stability of the high-precision displacement platform during high-speed operation. The X / Y axis dual slide design of the planar displacement platform, combined with servo motors and ball screw drives, achieves nanometer-level positioning accuracy of the film material carrying platform in the horizontal direction, ensuring no deviation in the spraying path.

[0016] Furthermore, the Y-axis center distance between the three piezoelectric micro-nozzles is 5-10 mm; the nozzle diameter of each nozzle is 50±5 μm, and the inner diameter of the independent microchannel is 0.5±0.05 mm.

[0017] By adopting the above technical solution, the center-to-center distance of the nozzles on the Y-axis is precisely matched to the chromatographic diffusion characteristics of the nitrocellulose membrane, ensuring that the three detection lines do not interfere with each other during the chromatography process and avoiding false negatives caused by reagent diffusion overlap.

[0018] Furthermore, the Z-axis lifting mechanism controls the height of the nozzle from the film surface to be 0.5-1.0 mm;

[0019] The piezoelectric micro-nozzle is connected to the corresponding liquid pump through the first hose connection port and the second hose connection port.

[0020] By adopting the above technical solution, the Z-axis lifting mechanism controls the suspension height of the nozzle from the membrane surface to 0.5-1.0 mm, completely avoiding membrane structure damage caused by the scratching needle and maintaining the original chromatography performance of the nitrocellulose membrane.

[0021] Furthermore, the cutting blade has a blade width of 25±1mm;

[0022] The downward pressure applied when the cutter is triggered is 5±0.5N, and the cutting width is 3.8-4.2mm.

[0023] By adopting the above technical solution, the blade width of the slitting blade covers the maximum width of the triple test strip, and with constant pressure control, burr-free slitting is achieved without damaging the pre-sprayed reagent layer.

[0024] Furthermore, the membrane material support platform is equipped with a vacuum adsorption structure, with an adsorption negative pressure of -0.06 to -0.1 MPa.

[0025] By adopting the above technical solution, the vacuum adsorption structure of the membrane material support platform achieves full-area flatness and fixation of the nitrocellulose membrane with negative pressure, overcoming the problem of spraying wrinkles caused by the deformation of the membrane material due to humidity.

[0026] Furthermore, the control system integrates an operation panel for setting:

[0027] nozzle Y-axis spacing parameters;

[0028] Spraying line width and speed;

[0029] Cut width and trigger delay time.

[0030] By adopting the above technical solution, the graphical control interface of the integrated operation screen integrates more than 20 parameters such as nozzle spacing, line width, and speed into preset process templates, which greatly reduces the operational complexity of multi-unit test card production.

[0031] Furthermore, at least one of the three piezoelectric micro-nozzles is detachable, forming a dual-detection spray film structure.

[0032] By adopting the above technical solution, the detachable nozzle is set with a modular slot structure to realize the quick replacement or removal of a single nozzle. At the same time, the disassembly interface adopts a self-sealing flow path, which automatically seals the microchannel when the nozzle is removed to prevent reagent leakage.

[0033] In summary, the present invention has the following main advantages:

[0034] 1. This invention utilizes a rigid, isolated layout of three piezoelectric micro-nozzles along the Y-axis to completely eliminate cross-contamination between different biological reagents from a physical structure perspective. The nozzles spray synchronously at preset intervals, ensuring that detection lines for diseases such as swine fever and foot-and-mouth disease are independently formed on the nitrocellulose membrane. This avoids false negatives caused by reagent diffusion and overlap in traditional membrane-scraping processes. Simultaneously, the non-contact spraying mode completely avoids scratching damage from the scrubbing needles, maintaining the integrity of the membrane's capillary structure and ensuring the accuracy of the test results. Furthermore, in conjunction with a control system, it achieves precise point spraying by the three piezoelectric micro-nozzles, thereby producing products such as… Figure 4 The test strip shown has three sets of test lines arranged in a stepped structure, which further avoids the problem that the concentration and effective binding capacity of the test liquid have decreased when it flows to the second and third test lines, thus reducing the sensitivity and lowering the probability of false negatives.

[0035] 2. This utility model provides the equipment with multi-scenario compatibility through the detachable nozzle design. By removing some nozzles, it can quickly switch to dual or single detection modes, adapting to different disease combination requirements without replacing the equipment. The control system's integrated operation screen has preset parameter templates, enabling mode switching operations to be completed in a short time, significantly reducing the conversion cost of multi-variety production. Attached Figure Description

[0036] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0037] Figure 2This is a schematic diagram of the main structure of this utility model;

[0038] Figure 3 This is a top view of the structure of this utility model;

[0039] Figure 4 This is a schematic diagram of the test strip structure produced by this utility model.

[0040] In the diagram: 1. Base; 2. Z-axis lifting mechanism; 3. Membrane material support platform; 4. Liquid pump; 5. First hose connection port; 6. Second hose connection port; 7. Top plate; 8. Piezoelectric micro-nozzle; 9. Blade; 10. Control panel. Detailed Implementation

[0041] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0042] In this embodiment:

[0043] A triple-coating machine for producing animal disease triple test cards, such as Figure 1-3 As shown, it includes: a rigid frame, a closed frame consisting of a base 1, a top plate 7 and side walls;

[0044] A high-precision planar displacement platform, integrated into base 1, includes:

[0045] The X-axis linear slide is driven by the first servo motor via a ball screw.

[0046] The Y-axis linear slide is orthogonally arranged above the X-axis slide and is driven by the second servo motor.

[0047] The membrane material support platform 3 is fixed to the Y-axis slide and is used to adsorb and fix the nitrocellulose membrane;

[0048] Z-axis lifting mechanism 2 is suspended below the top plate and is driven by a linear motor to achieve vertical movement via a precision guide rail.

[0049] A micro-fluid distribution unit, connected to the end of the Z-axis lifting mechanism 2, includes:

[0050] Three independent piezoelectric micro-nozzles 8 are rigidly arranged at a preset spacing in the Y-axis direction, and each nozzle is connected to a liquid pump 4 through an independent microchannel.

[0051] Stainless steel cutting blade 9 is fixed to the rear of the nozzle bracket along the negative Y-axis;

[0052] The control system is configured to synchronously drive the displacement platform, nozzle start / stop, and cutter action.

[0053] A robust, enclosed frame is constructed using a rigid chassis to ensure the mechanical stability of the high-precision displacement platform during high-speed operation. The X / Y axis dual-slide design of the planar displacement platform, combined with servo motors and ball screw drives, achieves nanometer-level positioning accuracy of the membrane material support platform 3 in the horizontal direction, ensuring no deviation in the spraying path. Meanwhile, the micro-fluid distribution unit innovatively employs three independent piezoelectric micro-nozzles 8 rigidly arranged along the Y-axis, completely eliminating the risk of cross-contamination between different biological reagents during the spraying process from a physical structure perspective. The slitting blade 9 integrated at the nozzle end is synchronously controlled by the Z-axis lifting mechanism 2, immediately slitting the test strips after spraying, integrating the traditionally separate spraying and slitting processes into a single continuous action, significantly improving the production efficiency and consistency of the triple test cards.

[0054] See Figure 1 , Figure 2 , Figure 3 The center-to-center distance between the three piezoelectric micro-nozzles 8 on the Y-axis is 5-10 mm; the nozzle diameter of each nozzle is 50±5 μm, and the inner diameter of the independent microchannel is 0.5±0.05 mm.

[0055] The precise matching of the Y-axis center spacing of the nozzles to the chromatographic diffusion characteristics of the nitrocellulose membrane ensures that the three detection lines do not interfere with each other during chromatography, avoiding false negatives caused by reagent diffusion overlap. At the same time, the coordinated design of the nozzle diameter and the microchannel inner diameter not only meets the high-precision non-contact spraying requirements of micro-reagents, but also avoids the blockage of biomolecules by optimizing fluid resistance.

[0056] See Figure 1 , Figure 2 , Figure 3 The Z-axis lifting mechanism 2 controls the height of the nozzle from the film surface to be 0.5-1.0 mm;

[0057] The piezoelectric micro-nozzle 8 is connected to the corresponding liquid addition pump 4 through the first hose connection port 5 and the second hose connection port 6. The Z-axis lifting mechanism 2 controls the suspension height of the nozzle from the membrane surface to be 0.5-1.0 mm, which completely avoids membrane structure damage caused by the scratching needle and maintains the original chromatography performance of the nitrocellulose membrane. At the same time, the independent microchannel is directly connected to the liquid addition pump 4 through the first and second hose connection ports to build a physically isolated closed flow path system, which prevents cross-contamination of different reagents in the delivery process, and is especially suitable for active and sensitive reagents.

[0058] See Figure 1 , Figure 2 , Figure 3 The blade width of the slicing blade 9 is 25±1mm;

[0059] The downward pressure applied when the cutter is triggered is 5±0.5N, and the cutting strip width is 3.8-4.2mm. The blade width of the cutting strip 9 covers the maximum width of the triple test strip. With constant pressure control, burr-free cutting is achieved without damaging the pre-sprayed reagent layer. At the same time, the cutting strip width is precisely matched with the standard test card size, avoiding secondary trimming. This cutting system is seamlessly connected with the spraying action through mechanical linkage, which improves the cutting efficiency of a single batch of test strips and eliminates the risk of membrane material contamination introduced by manual operation.

[0060] See Figure 1 , Figure 2 , Figure 3 The membrane material support platform 3 is equipped with a vacuum adsorption structure with an adsorption negative pressure of -0.06 to -0.1 MPa. The vacuum adsorption structure of the membrane material support platform 3 uses negative pressure to achieve full-area flat fixation of the nitrocellulose membrane, overcoming the problem of spraying wrinkles caused by membrane deformation due to humidity. At the same time, the uniform distribution of adsorption force ensures that the membrane material does not slide locally during high-speed displacement, which is especially suitable for ultra-thin NC membranes and avoids the blockage of chromatography channels caused by mechanical indentation.

[0061] See Figure 1 , Figure 2 , Figure 3 The control system integrates an operation panel 10 for setting:

[0062] nozzle Y-axis spacing parameters;

[0063] Spraying line width and speed;

[0064] Cut width and trigger delay time.

[0065] The graphical control interface of the integrated operation screen 10 integrates more than 20 parameters such as nozzle spacing, line width, and speed into preset process templates, which greatly reduces the operational complexity of multi-unit test card production. At the same time, the programmable setting of the cutting trigger delay time allows for dynamic adjustment of the cutting timing according to the drying characteristics of the reagent, avoiding the sticking of the cutter of uncured film material. This intelligent system shortens the time for switching test items to within 5 minutes, supporting flexible production.

[0066] See Figure 1 , Figure 2 , Figure 3 At least one of the three piezoelectric micro-nozzles is detachable, forming a dual-detection spray film structure. The detachable nozzles are set through a modular slot structure, which enables quick replacement or removal of a single nozzle. At the same time, the disassembly interface adopts a self-sealing flow path, which automatically seals the microchannel when the nozzle is removed to prevent reagent leakage. This extended structure makes a single device compatible with the production of a full range of detection cards from single to triplet, so users can meet the needs of different disease combinations without purchasing new equipment.

[0067] The implementation principle of this embodiment is as follows: During implementation, the nitrocellulose membrane is fixed on the membrane material support platform 3, and different biological reagents are supplied to three independent piezoelectric micro-nozzles 8 through the liquid pump 4; the control system drives the high-precision planar displacement platform to move the membrane material along the chromatography direction, while the Z-axis lifting mechanism 2 controls the nozzles to maintain a non-contact height. The three nozzles 8 spray synchronously at the Y-axis isolation spacing to form spatially isolated detection lines. After spraying, the cutting blade 9 immediately cuts the membrane material, and finally the base 1 integrates and collects the finished detection strips, realizing the efficient and cross-contamination-free production of the three-part detection card, thus producing the attached... Figure 4 The test strip shown;

[0068] like Figure 4 As shown, the test strip chromatography membrane has a first flow channel, a second flow channel, and a third flow channel that are physically isolated from each other; a first detection line is located on the first flow channel, a second detection line is located on the second flow channel, and a third detection line is located on the third flow channel. The first detection line, the second detection line, and the third detection line are arranged in a stepped gradient on the surface of the chromatography membrane, wherein the physical isolation between them is achieved through any of the following methods:

[0069] 1. The isolation trenches formed by laser etching have a depth of 0.1-0.3 mm and a width of 0.3-0.8 mm;

[0070] In this process, the etching machine is installed on one side of several piezoelectric micro-nozzles 8. In conjunction with the X / Y axis double slide table, the film material carrying platform is displaced, thereby realizing the simultaneous etching and spraying, and producing a new type of test strip. This provides another embodiment of the present application, further improving the production efficiency of test strips using the spraying machine and improving the ease of use of the spraying machine.

[0071] 2. Polyethylene terephthalate separator, with a thickness of 0.05-0.15 mm;

[0072] In some other embodiments, the piezoelectric micro-nozzle 8 is not limited to three sets. In necessary processing steps, a quality control line piezoelectric micro-nozzle can be added to the test strip and connected to an external quality control line raw material storage device via a corresponding liquid pump and hose to complete the spraying operation of the quality control line.

[0073] Although embodiments of the present invention have been shown and described, these specific embodiments are merely explanations of the present invention and are not intended to limit the invention. The specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions, and variations to the embodiments as needed without departing from the principles and spirit of the present invention, provided that such modifications, substitutions, and variations are within the scope of the claims of the present invention and are protected by patent law.

Claims

1. A triple-coating machine for producing animal disease triple test cards, characterized in that: include: A rigid frame, consisting of a base (1), a top plate (7) and side walls forming a closed frame; A high-precision planar displacement platform, integrated into a base (1), includes: The X-axis linear slide is driven by the first servo motor via a ball screw. The Y-axis linear slide is orthogonally arranged above the X-axis slide and is driven by the second servo motor. The membrane material support platform (3) is fixed to the Y-axis slide and is used to adsorb and fix the nitrocellulose membrane; Z-axis lifting mechanism (2) is suspended below the top plate and vertical movement is achieved by a linear motor driving a precision guide rail; A micro-fluid distribution unit, connected to the end of the Z-axis lifting mechanism (2), includes: Three independent piezoelectric micro-nozzles (8) are rigidly arranged at a preset spacing in the Y-axis direction, and each nozzle is connected to a liquid pump (4) through an independent microchannel. Stainless steel cutting blade (9) is fixed to the rear of the nozzle bracket along the negative Y-axis; The control system is configured to synchronously drive the displacement platform, nozzle start / stop, and cutter action.

2. The triple-coating machine for producing animal disease triple test cards according to claim 1, characterized in that: The center-to-center distance between the three piezoelectric micro-nozzles (8) on the Y-axis is 5-10 mm; the nozzle diameter of each nozzle is 50±5 μm, and the inner diameter of the independent microchannel is 0.5±0.05 mm.

3. The triple-coating apparatus for producing animal disease triple test cards according to claim 1, characterized in that: The Z-axis lifting mechanism (2) controls the height of the nozzle from the film surface to be 0.5-1.0 mm; The piezoelectric micro-nozzle (8) is connected to the corresponding liquid pump (4) through the first hose connection port (5) and the second hose connection port (6).

4. The triple-coating machine for producing animal disease triple test cards according to claim 1, characterized in that: The blade width of the slicing blade (9) is 25±1mm; The downward pressure applied when the cutter is triggered is 5±0.5N, and the cutting width is 3.8-4.2mm.

5. The triple-coating apparatus for producing animal disease triple test cards according to any one of claims 1-4, characterized in that: The membrane material support platform (3) is equipped with a vacuum adsorption structure, and the adsorption negative pressure is -0.06 to -0.1 MPa.

6. The triple-coating machine for producing animal disease triple test cards according to claim 1, characterized in that: The control system integrates an operation panel (10) for setting: nozzle Y-axis spacing parameters; Spraying line width and speed; Cut width and trigger delay time.

7. The triple-coating apparatus for producing animal disease triple test cards according to claim 1, characterized in that: At least one of the three piezoelectric micro-nozzles (8) is detachable, forming a dual-detection spray film structure.

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