A device for efficiently cleaning an enzyme-labeled plate

Abstract

The utility model discloses a kind of high-efficiency cleaning enzyme mark plate's device, including washing tank, and the lid of hinged rotation installation in the middle of washing tank top end, and the inside of washing tank is provided with enzyme mark plate body, further include: for the medium switching circulation flushing mechanism of flushing to enzyme mark plate body, medium switching circulation flushing mechanism includes the reciprocating slide rail being positioned in the inside of washing tank and being located below position of enzyme mark plate body, and the water box being installed in the bottom end of washing tank inner wall and corresponding with reciprocating slide rail position. Through medium switching circulation flushing mechanism, the way of three-way pipe, pure water intercepting valve, washing liquid intercepting valve collaborative control is utilized, pure water storage tank, washing liquid storage tank independent liquid supply structure is utilized, the quick switching supply of pure water and washing liquid can be realized, avoid artificial disassembly pipeline and container replacement operation, ensure the continuity of cleaning process, solved the problem that experiment personnel must repeatedly manual intervention in prior art due to double liquid path alternate use.

Description

Technical Field

[0001] This utility model relates to the field of enzyme-labeled plate cleaning technology, and in particular to a device for efficient cleaning of enzyme-labeled plates. Background Technology

[0002] An ELISA plate is a porous microplate that serves as the core carrier for enzyme-linked immunosorbent assays (ELISA). It is typically made of inert polymers such as polystyrene, with its surface immobilized by physical adsorption or chemical modification of specific antigens or antibodies. This micro-well array structure provides independent reaction sites for biomolecular interactions. The experiment sequentially carries out steps such as sample addition, enzyme label binding, and substrate color development. Qualitative or quantitative analysis of the analyte is achieved through the quantifiable signal generated by enzyme catalysis of the substrate. Its standardized design ensures the homogeneity of the reaction system, and combined with spectroscopic detection equipment, it enables high-throughput screening. It is widely used in clinical diagnostics, biological research, and drug development. During the use of ELISA plates, the cleaning step is crucial for ensuring detection accuracy. By removing unbound free components, background signal interference can be effectively reduced, false positive or false negative results can be avoided, and cross-contamination between wells can be prevented. This ensures the independence of each reaction well and the repeatability of results, ultimately improving the signal-to-noise ratio and data reliability of the detection system.

[0003] Existing ELISA plate cleaning equipment suffers from a liquid switching issue due to the independent piping design of the washing and rinsing systems. This necessitates repeated manual intervention by researchers during the plate washing process. Specifically, when performing the sample washing procedure, researchers must first switch the pure water supply line to a dedicated washing solution delivery channel. Then, during the rinsing phase, they must reverse the process to switch the line back to pure water supply. This dual-liquid-path alternation mechanism involves complex steps such as pipe disassembly and container replacement for each liquid switch, compromising the continuity of the cleaning process and reducing overall cleaning efficiency. Therefore, this paper proposes a device for efficiently cleaning ELISA plates. Utility Model Content

[0004] In order to overcome the shortcomings of the existing technology, this utility model proposes a device for efficiently cleaning enzyme-labeled plates.

[0005] To solve the above-mentioned technical problems, the basic technical solution proposed by this utility model is as follows:

[0006] An efficient device for cleaning ELISA plates includes a cleaning chamber and a cover that is hinged and rotated at the top center of the cleaning chamber. The cleaning chamber contains an ELISA plate body. The device also includes a media switching and circulating rinsing mechanism for rinsing the ELISA plate body. The media switching and circulating rinsing mechanism includes a reciprocating slide rail located inside the cleaning chamber and below the ELISA plate body, and a water box installed at the bottom of the inner wall of the cleaning chamber and corresponding to the position of the reciprocating slide rail.

[0007] Preferably, the inner wall of one end of the reciprocating slide rail is provided with a slide rail guide rod for correcting the reciprocating slide rail, and the inner wall of the other end of the reciprocating slide rail is provided with a reciprocating lead screw for reciprocating traction of the reciprocating slide rail. The slide rail guide rod and the reciprocating lead screw are parallel to each other. The slide rail guide rod is connected to the cleaning box. The reciprocating slide rail and the slide rail guide rod are slidably arranged. The reciprocating lead screw is rotatably arranged with the cleaning box. The outer end of the reciprocating lead screw is connected and assembled to the output end of the external drive component through a coupling.

[0008] Preferably, a scissor-type telescopic assembly is provided in the middle of the reciprocating slide rail, and a flushing nozzle is provided at the intersection center point of each scissor arm of the scissor-type telescopic assembly. Traction sliders are symmetrically arranged at both ends of the inner wall of the reciprocating slide rail. Both ends of the scissor-type telescopic assembly are rotatably connected to the two traction sliders via a transition shaft. A telescopic hose is provided at the bottom of the flushing nozzle, and both ends of the telescopic hose are connected to the flushing nozzle and the water box, respectively. A guide pipe is installed at the bottom of the water box. The flushing nozzle, telescopic hose, water box, and guide pipe are connected in series.

[0009] Preferably, the two ends of the inner wall of the reciprocating slide rail are respectively provided with a slider guide rod and a bidirectional screw rod, and the slider guide rod and the bidirectional screw rod are parallel to each other. The slider guide rod is connected to the reciprocating slide rail. Both traction sliders are slidably arranged with the slider guide rod. The bidirectional screw rod is rotatably arranged with the reciprocating slide rail. Both traction sliders are engaged with the bidirectional screw rod by threads. A knob is fixedly arranged in the middle of the outer end of the bidirectional screw rod.

[0010] Preferably, the media switching and circulating flushing mechanism further includes a pure water storage tank and a washing liquid storage tank installed at both ends of the same outer wall of the cleaning tank, a three-way pipe disposed between the pure water storage tank and the washing liquid storage tank, and a pump body located at the position of the three-way pipe, wherein the outlet end of the pump body is connected to the inlet end of the guide pipe, and the inlet end of the pump body is connected to the outlet end in the middle of the three-way pipe.

[0011] Preferably, a pure water shut-off valve is installed at the end of the three-way pipe facing the pure water storage tank, and a detergent shut-off valve is installed at the end of the three-way pipe facing the detergent storage tank. The inlet end of the pure water shut-off valve is connected to the outlet end at the bottom of the pure water storage tank, and the inlet end of the detergent shut-off valve is connected to the outlet end at the bottom of the detergent storage tank. A pure water inlet is provided on the top of the pure water storage tank, and a detergent inlet is provided on the top of the detergent storage tank.

[0012] Preferably, horizontal support plates for supporting the enzyme-labeled plate body are symmetrically installed at both ends of the upper inner wall of the washing box, and a drain outlet for discharging wastewater is opened through the middle of the bottom of the inner wall of the washing box, and a drain cover is rotatably installed at the bottom of the outer wall of the washing box at the drain outlet position via a rotating shaft.

[0013] The beneficial effects of this utility model are:

[0014] By setting up a media switching and circulating rinsing mechanism, and using a three-way pipe, a pure water shut-off valve, and a washing liquid shut-off valve for coordinated control, and utilizing an independent liquid supply structure for the pure water storage tank and the washing liquid storage tank, it is possible to quickly switch between pure water and washing liquid supply, avoid manual disassembly of pipelines and container replacement operations, ensure the continuity of the cleaning process, and solve the problem in the existing technology that requires repeated manual intervention by experimental personnel due to the alternating use of dual liquid paths. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the internal structure of the cleaning box of this utility model. Figure 1 ;

[0017] Figure 3 This is a schematic diagram of the internal structure of the cleaning box of this utility model. Figure 2 ;

[0018] Figure 4 This is a schematic diagram of the media switching and circulating flushing mechanism of this utility model.

[0019] Explanation of reference numerals in the attached figures:

[0020] 100. Cleaning tank; 101. Horizontal support plate; 102. Drain port; 103. Drain port cover; 104. Rotating shaft; 200. Media switching and circulating flushing mechanism; 201. Pure water storage tank; 202. Washing solution storage tank; 203. Reciprocating slide rail; 204. Water box; 205. Guide pipe; 206. Pure water inlet; 207. Pure water shut-off valve; 208. T-connector; 209. Washing solution shut-off valve; 210. Pump body; 211. Washing solution inlet; 212. Slide rail guide rod; 213. Reciprocating lead screw; 214. Telescopic hose; 215. Knob; 216. Adapter shaft; 217. Flushing nozzle; 218. Scissor-type telescopic assembly; 219. Bidirectional screw; 220. Traction slider; 221. Slider guide rod; 300. Box cover; 400. ELISA plate body. Detailed Implementation

[0021] The following will be combined with the appendix Figure 1 To be continued Figure 4 The technical solutions in the embodiments of this utility model are clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this utility model.

[0022] This utility model provides a technical solution: a device for efficiently cleaning ELISA plates, including a cleaning chamber 100 and a cover 300 hinged to the top center of the cleaning chamber 100. The cover 300 is made of transparent glass material, which facilitates the experimenter to observe the internal condition of the cleaning chamber 100 in real time. The cleaning chamber 100 contains an ELISA plate body 400, with the front side of the ELISA plate body 400 facing down and the back side facing up, which facilitates rinsing with pure water and washing solution. The device also includes a media switching and circulating rinsing mechanism 200 for rinsing the ELISA plate body 400.

[0023] Specifically, the media switching and cyclic rinsing mechanism 200 includes a reciprocating slide rail 203 located inside the washing tank 100 and below the ELISA plate body 400, and a water tank 204 installed at the bottom of the inner wall of the washing tank 100, corresponding to the position of the reciprocating slide rail 203. A guide rod 212 for correcting the reciprocating slide rail 203 is provided on the inner wall of one end of the reciprocating slide rail 203, and a reciprocating lead screw 213 for reciprocating traction of the reciprocating slide rail 203 is provided on the inner wall of the other end of the reciprocating slide rail 203. The guide rod 212 and the reciprocating lead screw 213 are parallel to each other. The guide rod 212 is connected to the washing tank 100, the reciprocating slide rail 203 is slidably disposed with the guide rod 212, and the reciprocating lead screw 213 is rotatably disposed with the washing tank 100. The outer end of the reciprocating lead screw 213 is connected to the output end of the external drive unit via a coupling. Using the reciprocating lead screw 213, the reciprocating slide rail 203 can be driven to slide back and forth along the axial trajectory of the slide rail guide rod 212. The principle of this is prior art and will not be elaborated here. The drive unit, i.e., the electric motor, provides driving force to the reciprocating lead screw 213. It is not shown or labeled in the accompanying drawings and will not be elaborated here as well. A scissor-type telescopic assembly 218 is provided in the middle of the reciprocating slide rail 203. Each cross-center point of the scissor arms of the scissor-type telescopic assembly 218 is equipped with a rinsing nozzle 217, used to spray pure water or washing water upwards to rinse the micropores on the front side of the ELISA plate body 400. The rinsing nozzle 217 is connected to the ELISA plate body. Corresponding to the micropores on the front of the body 400, the scissor-type telescopic assembly 218 allows for equidistant adjustment of each rinsing nozzle 217 to accommodate different sizes of the ELISA plate body 400. The rinsing nozzles 217 are in an active position at the intersection of the scissor arms, ensuring the normal extension and retraction of the scissor-type telescopic assembly 218. Traction sliders 220 are symmetrically arranged at both ends of the inner wall of the reciprocating slide rail 203 for synchronously pulling the two ends of the scissor-type telescopic assembly 218. Both ends of the scissor-type telescopic assembly 218 and the two traction sliders 220 are rotatably connected via a transition shaft 216. A slider guide rod 221 and a bidirectional screw 219 are respectively arranged at both ends of the inner wall of the reciprocating slide rail 203. Parallel to each other, the slider guide rod 221 is connected to the reciprocating slide rail 203. Two traction sliders 220 are slidably mounted on the slider guide rod 221. The bidirectional screw 219 is rotatably mounted on the reciprocating slide rail 203. Both traction sliders 220 are threaded into the bidirectional screw 219. A knob 215 is fixedly mounted at the middle of the outer end of the bidirectional screw 219. A telescopic hose 214 is mounted at the bottom of the rinsing nozzle 217 to deliver pure water or washing liquid from the water tank 204 to the rinsing nozzle 217. The two ends of the telescopic hose 214 are connected to the rinsing nozzle 217 and the water tank 204 respectively. The length of the telescopic hose 214 is sufficient to ensure that the reciprocating slide rail 203 does not break during reciprocating movement. A guide pipe 205 is installed at the bottom of the water tank 204.The flushing nozzle 217, telescopic hose 214, water tank 204, and guide pipe 205 are connected.

[0024] Specifically, the media switching circulation flushing mechanism 200 also includes a pure water storage tank 201 and a washing liquid storage tank 202 installed at both ends of the same outer wall of the cleaning tank 100, a three-way pipe 208 disposed between the pure water storage tank 201 and the washing liquid storage tank 202, and a pump body 210 located at the position of the three-way pipe 208. The outlet end of the pump body 210 is connected to the inlet end of the guide pipe 205, and the inlet end of the pump body 210 is connected to the outlet end in the middle of the three-way pipe 208. The three-way pipe 208 is directly facing... A pure water storage tank 201 is equipped with a pure water shut-off valve 207 at one end, and a detergent shut-off valve 209 is installed at the other end of the three-way pipe 208 facing the detergent storage tank 202. The inlet end of the pure water shut-off valve 207 is connected to the outlet end at the bottom of the pure water storage tank 201, and the inlet end of the detergent shut-off valve 209 is connected to the outlet end at the bottom of the detergent storage tank 202. A pure water inlet 206 is provided on the top of the pure water storage tank 201, and a detergent inlet 211 is provided on the top of the detergent storage tank 202.

[0025] Specifically, horizontal support plates 101 for supporting the enzyme-labeled plate body 400 are symmetrically installed at both ends of the upper inner wall of the washing box 100, which facilitates the experimenter to place the enzyme-labeled plate body 400 horizontally. A drain port 102 for discharging wastewater is opened through the middle of the bottom of the inner wall of the washing box 100. A drain cover 103 is rotatably installed at the bottom of the outer wall of the washing box 100 and at the position of the drain port 102 via a rotating shaft 104 to seal the drain port 102. The drain cover 103 is made of rubber material.

[0026] Based on the above, this utility model, by setting up a media switching circulation rinsing mechanism 200, utilizes a three-way pipe 208, a pure water shut-off valve 207, and a washing liquid shut-off valve 209 for coordinated control, and employs an independent liquid supply structure for the pure water storage tank 201 and the washing liquid storage tank 202. This enables rapid switching between pure water and washing liquid supply, avoiding manual disassembly of pipelines and container replacement operations, ensuring the continuity of the cleaning process, and solving the problem in existing technologies where repeated manual intervention by experimental personnel is required due to the alternating use of dual liquid paths. Furthermore, by setting up a reciprocating slide rail 203 and a reciprocating lead screw 213, the reciprocating lead screw 213... The reciprocating slide rail 203 is driven to slide back and forth along the axial trajectory of the slide rail guide rod 212, which enables the rinsing nozzle 217 to perform periodic reciprocating motion in the area below the ELISA plate body 400. This ensures that the cleaning medium fully covers all micropores on the front of the ELISA plate, avoiding cleaning dead corners. Furthermore, by setting up a scissor-type telescopic component 218 and a bidirectional screw 219, and by using the knob 215 to adjust the bidirectional screw 219 to drive the two traction sliders 220 to move synchronously, the spacing of the rinsing nozzle 217 can be dynamically adjusted according to the specifications of the ELISA plate body 400, ensuring that the rinsing nozzle 217 always corresponds to the position of the micropores.

[0027] Based on the disclosure and teachings of the above specification, those skilled in the art can make changes and modifications to the above embodiments. Therefore, this utility model is not limited to the specific embodiments disclosed and described above, and some modifications and changes to this utility model should also fall within the protection scope of the claims of this utility model. Furthermore, although some specific terms are used in this specification, these terms are only for convenience of explanation and do not constitute any limitation on this utility model.

Claims

1. A device for efficiently cleaning ELISA plates, comprising a cleaning chamber (100) and a cover (300) hinged to the center of the top of the cleaning chamber (100), wherein the cleaning chamber (100) contains an ELISA plate body (400), characterized in that, It also includes a media switching and cyclic rinsing mechanism (200) for rinsing the ELISA plate body (400), the media switching and cyclic rinsing mechanism (200) includes a reciprocating slide rail (203) disposed inside the washing tank (100) and located below the ELISA plate body (400), and a water box (204) installed at the bottom of the inner wall of the washing tank (100) and corresponding to the position of the reciprocating slide rail (203).

2. The apparatus for efficiently cleaning ELISA plates according to claim 1, characterized in that: The inner wall of one end of the reciprocating slide rail (203) is provided with a slide rail guide rod (212) for correcting the reciprocating slide rail (203), and the inner wall of the other end of the reciprocating slide rail (203) is provided with a reciprocating screw (213) for reciprocating traction of the reciprocating slide rail (203). The slide rail guide rod (212) and the reciprocating screw (213) are parallel to each other. The slide rail guide rod (212) is connected to the cleaning box (100). The reciprocating slide rail (203) and the slide rail guide rod (212) are slidably arranged. The reciprocating screw (213) is rotatably arranged with the cleaning box (100). The outer end of the reciprocating screw (213) is connected and assembled with the output end of the external drive component through a coupling.

3. The apparatus for efficiently cleaning ELISA plates according to claim 1, characterized in that: A scissor-type telescopic assembly (218) is provided in the middle of the reciprocating slide rail (203), and a flushing nozzle (217) is provided at the intersection center point of each scissor arm of the scissor-type telescopic assembly (218). Traction sliders (220) are symmetrically arranged at both ends of the inner wall of the reciprocating slide rail (203). Both ends of the scissor-type telescopic assembly (218) are rotatably arranged with the two traction sliders (220) through the adapter shaft (216). A telescopic hose (214) is provided at the bottom end of the flushing nozzle (217), and both ends of the telescopic hose (214) are connected to the flushing nozzle (217) and the water box (204) respectively. A guide pipe (205) is installed at the bottom of the water box (204). The flushing nozzle (217), the telescopic hose (214), the water box (204), and the guide pipe (205) are connected.

4. The apparatus for efficiently cleaning ELISA plates according to claim 3, characterized in that: The inner walls of the reciprocating slide rail (203) are respectively provided with a slider guide rod (221) and a bidirectional screw rod (219) at both ends, and the slider guide rod (221) and the bidirectional screw rod (219) are parallel to each other. The slider guide rod (221) is connected to the reciprocating slide rail (203). The two traction sliders (220) are slidably arranged with the slider guide rod (221). The bidirectional screw rod (219) is rotatably arranged with the reciprocating slide rail (203). The two traction sliders (220) are engaged with the bidirectional screw rod (219) by threads. A knob (215) is fixedly arranged in the middle of the outer end of the bidirectional screw rod (219).

5. The apparatus for efficiently cleaning ELISA plates according to claim 1, characterized in that: The media switching circulation flushing mechanism (200) further includes a pure water storage tank (201) and a washing liquid storage tank (202) installed at both ends of the same outer wall of the cleaning tank (100), a three-way pipe (208) disposed between the pure water storage tank (201) and the washing liquid storage tank (202), and a pump body (210) located at the position of the three-way pipe (208), wherein the outlet end of the pump body (210) is connected to the inlet end of the guide pipe (205), and the inlet end of the pump body (210) is connected to the outlet end in the middle of the three-way pipe (208).

6. The apparatus for efficiently cleaning ELISA plates according to claim 5, characterized in that: A pure water shut-off valve (207) is installed at the end of the three-way pipe (208) facing the pure water storage tank (201), and a washing liquid shut-off valve (209) is installed at the end of the three-way pipe (208) facing the washing liquid storage tank (202). The inlet end of the pure water shut-off valve (207) is connected to the outlet end at the bottom of the pure water storage tank (201), and the inlet end of the washing liquid shut-off valve (209) is connected to the outlet end at the bottom of the washing liquid storage tank (202). A pure water inlet (206) is provided on the top of the pure water storage tank (201), and a washing liquid inlet (211) is provided on the top of the washing liquid storage tank (202).

7. The apparatus for efficiently cleaning ELISA plates according to claim 1, characterized in that: The upper half of the inner wall of the washing box (100) is symmetrically equipped with horizontal support plates (101) for supporting the enzyme labeling plate body (400) at both ends. A drain port (102) for discharging wastewater is opened through the middle of the bottom of the inner wall of the washing box (100). A drain cover (103) is rotatably installed at the bottom of the outer wall of the washing box (100) at the drain port (102) via a rotating shaft (104).

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