Automatic polypeptide solid carrier loading and cleaning device

Abstract

The utility model relates to polypeptide processing technical field discloses a kind of polypeptide solid phase carrier automatic filling and cleaning device, including cylinder, the upper end surface of cylinder is passed through and is provided with lifting assembly, the side of cylinder is provided with filling assembly, and the inside of cylinder is provided with cleaning assembly, and the drive end of lifting assembly is connected in cleaning assembly;Through the cooperation of motor, rotating lever, paddle and filter plate, polypeptide solid phase carrier is agitated, and its surface is cleaned, simultaneously using the cooperation of hydraulic rod and mounting plate, polypeptide solid phase carrier can be separated from liquid and moved to square tube, so that polypeptide solid phase carrier is filled to the inside of reaction equipment by adapter and elbow, and then the process to polypeptide solid phase carrier is completed, and polypeptide solid phase carrier can be cleaned, avoid impurity residue, ensure the quality when polypeptide production.

Description

Technical Field

[0001] This utility model relates to the field of polypeptide processing technology, and more specifically, to an automatic filling and cleaning device for polypeptide solid-phase carriers. Background Technology

[0002] Peptide processing refers to the synthesis, modification, or processing of peptide molecules through chemical, enzymatic, or biological methods. It is commonly used in research, pharmaceuticals, food, and cosmetics. With the rapid development of biotechnology and the pharmaceutical industry, peptide synthesis technology has attracted much attention due to its wide application in peptide drug development and scientific research. Solid-phase peptide synthesis (SPPS), as a highly efficient and high-purity synthesis method, has become an industry standard by sequentially coupling amino acids onto a solid support. However, this process is highly dependent on equipment, involving key steps such as solid support loading, cleaning, and peptide cleavage. Traditional solid-phase synthesis equipment often relies on manual operation, resulting in problems such as uneven support loading and low cleaning efficiency, leading to decreased peptide yield and increased production costs.

[0003] In the process of processing peptides, a filling device is required to fill the peptide solid carrier. In the existing technology, the solid carrier usually relies on external cleaning equipment for cleaning, which cannot be synchronized with the filling process. This can easily introduce impurities, resulting in impurity residues on the surface of the solid carrier, which in turn affects the quality of the peptide during processing.

[0004] Therefore, there is a need to provide an automatic loading and cleaning device for polypeptide solid-phase carriers to solve the problem that existing polypeptide processing methods easily lead to the formation of impurities on the surface of solid-phase carriers. Utility Model Content

[0005] The main objective of this invention is to provide an automatic loading and cleaning device for polypeptide solid-phase carriers, which aims to solve the technical problems mentioned in the background section.

[0006] The present invention adopts the following technical solution:

[0007] An automatic filling and cleaning device for polypeptide solid-phase carriers includes: a cylinder, a lifting component is provided through the upper end face of the cylinder, a filling component is provided on one side of the cylinder, and a cleaning component is provided inside the cylinder, the cleaning component being connected to the drive end of the lifting component;

[0008] The cleaning assembly includes a motor disposed inside the cylinder, a rotating rod fixedly installed at the output end of the motor, a set of paddles fixedly installed on the outer surface of the rotating rod, and a filter plate rotatably installed at the bottom end of the rotating rod via a bearing.

[0009] Furthermore, the filling assembly includes a square tube installed on the right side of the cylinder, the right end of the square tube being fixedly connected to an adapter, and the output end of the adapter being fixedly connected to a bent pipe.

[0010] Furthermore, a nitrogen inlet pipe is fixedly connected to the upper surface of the square tube, a connecting joint is fixedly connected to the top end of the nitrogen inlet pipe, a perforated diverter box is fixedly connected to the bottom end of the nitrogen inlet pipe, an opening is opened on the bottom surface of the square tube, and a filter screen is fixedly installed on the inner wall of the opening.

[0011] Furthermore, the lifting assembly includes a hydraulic rod installed on the upper surface of the cylinder, a mounting plate is fixedly installed on the telescopic end of the hydraulic rod, the upper surface of the motor is fixedly installed on the bottom surface of the mounting plate, and a control panel is fixedly installed on the upper surface of the cylinder.

[0012] Furthermore, two sets of support legs are provided at the bottom of the cylinder, and the upper surface of each support leg is fixedly installed to the bottom surface of the cylinder.

[0013] Furthermore, a feed inlet is provided on the upper surface of the cylinder, and a sealing plug is threaded onto the inner wall of the feed inlet.

[0014] Furthermore, a drain pipe is fixedly connected to the inner bottom wall of the cylinder, and a solenoid valve is fixedly connected to the bottom end of the drain pipe.

[0015] Furthermore, the inner wall of the cylinder is provided with two sets of guide grooves, and a guide block is slidably installed on the inner wall of each guide groove. The side of the two sets of guide blocks that are close to each other is fixedly installed on the outer surface of the filter plate.

[0016] Beneficial effects:

[0017] This invention provides an automatic loading and cleaning device for polypeptide solid carriers. Through the cooperation of a motor, rotating rod, deflector, and filter plate, the polypeptide solid carrier is agitated and its surface cleaned. Simultaneously, the cooperation of a hydraulic rod and mounting plate separates the polypeptide solid carrier from the liquid, allowing it to move to a square tube. This facilitates the entry of the polypeptide solid carrier into the square tube under the action of the deflector. The cooperation of a nitrogen inlet pipe, a perforated distribution box, and a filter screen dries any residual solution on the polypeptide solid carrier. The polypeptide solid carrier is then loaded into the reaction equipment through an adapter and a bend, thus completing the process of cleaning the polypeptide solid carrier, preventing impurities, and ensuring the quality of polypeptide production. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of an automatic filling and cleaning device for polypeptide solid-phase carriers according to this utility model.

[0019] Figure 2This is a top view cross-sectional structural diagram of the present invention;

[0020] Figure 3 This is a frontal cross-sectional structural diagram of the present invention;

[0021] Figure 4 yes Figure 3 Enlarged structural diagram at point A;

[0022] The components include: 1. Cylinder; 101. Support leg; 102. Feed inlet; 103. Sealing plug; 2. Lifting assembly; 201. Hydraulic rod; 202. Mounting plate; 203. Control panel; 3. Filling assembly; 301. Square tube; 302. Adapter; 303. Bend; 304. Connecting joint; 305. Perforated diverter box; 306. Nitrogen inlet pipe; 307. Port; 308. Filter screen; 4. Cleaning assembly; 401. Motor; 402. Rotating rod; 403. Paddle; 404. Filter plate; 5. Guide groove; 501. Guide block; 6. Drain pipe; 601. Solenoid valve.

[0023] The realization of the purpose, functional features and advantages of this utility model will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0024] It should be understood that the specific embodiments described herein are merely illustrative of the present invention and are not intended to limit the present invention.

[0025] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined with "first" or "second" may explicitly or implicitly include one or more of the stated features. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0026] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection, a direct connection, or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0027] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0028] Reference Figures 1 to 3 This utility model proposes an automatic filling and cleaning device for polypeptide solid carriers, comprising: a cylinder 1, a lifting component 2 being provided through the upper end face of the cylinder 1, a filling component 3 being provided on one side of the cylinder 1, and a cleaning component 4 being provided inside the cylinder 1, the cleaning component 4 being connected to the driving end of the lifting component 2.

[0029] The cleaning assembly 4 includes a motor 401 disposed inside the cylinder 1. A rotating rod 402 is fixedly installed at the output end of the motor 401. A set of paddles 403 are fixedly installed on the outer surface of the rotating rod 402. A filter plate 404 is rotatably installed at the bottom end of the rotating rod 402 through a bearing.

[0030] In the above embodiments, the automated cleaning and filling of the peptide solid-phase carrier is achieved through the synergistic action of the cylinder 1, lifting assembly 2, filling assembly 3, and cleaning assembly 4. Specifically, the cylinder 1 serves as the main container, holding the peptide solid-phase carrier and the cleaning solution. The lifting assembly 2 is installed on the upper surface of the cylinder 1 and moves up and down by driving the cleaning assembly 4, thus facilitating the cleaning and transfer of the solid-phase carrier. The filling assembly 3 is located on one side of the cylinder 1 and fills the cleaned solid-phase carrier into the reaction equipment. The cleaning assembly 4 is connected to the lifting assembly 2 through its drive end, ensuring efficient stirring and separation of the solid-phase carrier during the cleaning process.

[0031] The effective cleaning of the peptide solid-phase carrier is achieved through the cooperation of motor 401, rotating rod 402, levers 403, and filter plate 404. Motor 401 is fixedly installed inside the cylinder 1, and its output end is connected to rotating rod 402. A set of levers 403 is fixed to the outer surface of rotating rod 402, and the bottom end of rotating rod 402 is connected to filter plate 404 via a bearing. Filter plate 404 serves as a support platform for the solid-phase carrier, maintaining stability during the rotation of rotating rod 402 while allowing the cleaning solution to pass through.

[0032] After the motor 401 starts, it drives the rotating rod 402 to rotate. The paddle 403 on the rotating rod 402 rotates accordingly, stirring the polypeptide solid carrier on the filter plate 404 to ensure full contact with the cleaning solution, thereby removing surface impurities and residues. The filter plate 404, connected to the rotating rod 402, ensures that it will not tilt or shift when the rotating rod 402 rotates at high speed, thus guaranteeing the stability of the cleaning process. In addition, the porous structure of the filter plate 404 allows the cleaning solution to flow out through the filter plate 404 after stirring, facilitating solid-liquid separation. Subsequently, the lifting component 2 moves the cleaning component 4 upward, lifting the cleaned solid carrier to the inlet position of the filling component 3. The filling component 3 smoothly transfers the solid carrier to the external reaction equipment through its conveying channel. The entire process is automated through the control system, reducing manual intervention and improving production efficiency. This embodiment solves the problems of low cleaning and filling efficiency and easy residue of impurities in traditional polypeptide solid carriers through the reasonable layout and coordinated work of each component, providing reliable technical support for polypeptide production.

[0033] refer to Figure 2 and Figure 4 In one embodiment, the filling assembly 3 includes a square tube 301 installed on the right side of the cylinder 1. The right end of the square tube 301 is fixedly connected to an adapter 302, and the output end of the adapter 302 is fixedly connected to a bend 303. The upper surface of the square tube 301 is fixedly connected to a nitrogen inlet pipe 306. The top end of the nitrogen inlet pipe 306 is fixedly connected to a connector 304, and the bottom end of the nitrogen inlet pipe 306 is fixedly connected to a perforated distribution box 305. The bottom surface of the square tube 301 has an opening 307, and a filter screen 308 is fixedly installed on the inner wall of the opening 307.

[0034] In the above embodiment, the automated loading of the cleaned polypeptide solid carrier is achieved through a square tube 301, an adapter 302, and a bend 303. The square tube 301 is fixed to the right side of the cylinder 1, and its right end is connected to the bend 303 via the adapter 302, forming a continuous conveying channel. A nitrogen inlet pipe 306 is connected to the upper surface of the square tube 301, and a perforated distribution box 305 is connected to the bottom end of the nitrogen inlet pipe 306. A through-hole 307 is provided on the bottom surface of the square tube 301, and a filter screen 308 is installed inside the through-hole 307. The cleaned polypeptide solid carrier is transferred to the inlet of the square tube 301. Nitrogen gas enters the perforated distribution box 305 through the nitrogen inlet pipe 306, and the distribution box evenly sprays nitrogen gas onto the flowing solid carrier below, drying any residual cleaning solution on its surface. The filter screen 308 prevents the solid carrier from scattering from the through-hole 307 during nitrogen purging, while allowing gas and a small amount of liquid to pass through. The dried solid support continues forward along the square tube 301, enters the bend tube 303 through the adapter 302, and is finally transported to the reaction equipment. This embodiment, through the combination of nitrogen drying and pipeline transport, ensures that the solid support remains dry and clean during the filling process, avoiding the impact of solution residue on the quality of peptide production.

[0035] refer to Figure 3 In one example, the lifting assembly 2 includes a hydraulic rod 201 mounted on the upper surface of the cylinder 1, with a mounting plate 202 fixedly mounted on the telescopic end of the hydraulic rod 201, the upper surface of the motor 401 fixedly mounted to the bottom surface of the mounting plate 202, and a control panel 203 fixedly mounted on the upper surface of the cylinder 1.

[0036] In the above embodiment, the precise lifting and lowering of the cleaning assembly 4 is achieved through the cooperation of the hydraulic rod 201, the mounting plate 202, and the control panel 203. The hydraulic rod 201 is installed on the upper surface of the cylinder 1, and its telescopic end is fixedly connected to the mounting plate 202. The motor 401 of the cleaning assembly 4 is fixed to the bottom surface of the mounting plate 202. The upper surface of the cylinder 1 is also provided with a control panel 203 for controlling the movement of the hydraulic rod 201. The control panel 203 issues a command, and the hydraulic rod 201 extends or retracts as needed, driving the mounting plate 202 and the cleaning assembly 4 fixed thereon to move up and down. When it is necessary to transfer the cleaned solid carrier to the filling assembly 3, the hydraulic rod 201 retracts, causing the mounting plate 202 to lift the cleaning assembly 4 until the filter plate 404 is flush with the inlet of the filling assembly 3. Subsequently, the cleaning assembly 4 pushes the solid carrier into the filling assembly 3 through its stirring mechanism. The high-precision control of the hydraulic rod 201 ensures the smoothness and positioning accuracy of the lifting process, avoiding the scattering or loss of the solid carrier during the transfer process.

[0037] refer to Figure 1 In one embodiment, two sets of support legs 101 are provided below the cylinder 1, and the upper surface of each support leg 101 is fixedly installed to the bottom surface of the cylinder 1.

[0038] In the above embodiment, two sets of support legs 101 are fixed to the bottom surface of the cylinder 1. The upper surface of each set of support legs 101 is connected to the bottom surface of the cylinder 1 by welding or bolting to ensure sturdiness. The support legs 101 elevate the cylinder 1 to an appropriate height, providing space for wastewater collection and device maintenance. After cleaning, the wastewater is discharged through the drain pipe 6, and the support legs 101 allow the wastewater collection container to be easily placed under the cylinder 1. Furthermore, the stable structure of the support legs 101 can withstand the vibration and weight load during device operation, ensuring the stability of the device.

[0039] In one embodiment, the upper surface of the cylinder 1 is provided with a feed inlet 102, and the inner wall of the feed inlet 102 is threaded with a sealing plug 103.

[0040] In the above embodiment, the polypeptide solid carrier is dispensed through the inlet 102 and the sealing plug 103. The inlet 102 is located on the upper surface of the cylinder 1, and the inner wall of the inlet 102 is threaded, with the sealing plug 103 connected to the thread to seal the inlet 102. The operator unscrews the sealing plug 103 and adds the polypeptide solid carrier and cleaning solution into the cylinder 1 through the inlet 102. After dispensing, the sealing plug 103 is tightened. The threaded connection provides a seal, preventing foreign objects or dust from entering the cylinder 1 and avoiding solution splashing during the cleaning process.

[0041] refer to Figure 3 In one embodiment, a drain pipe 6 is fixedly connected to the inner bottom wall of the cylinder 1, and a solenoid valve 601 is fixedly connected to the bottom end of the drain pipe 6.

[0042] In the above embodiment, the effective discharge of cleaning wastewater is achieved through the cooperation of the drain pipe 6 and the solenoid valve 601. The drain pipe 6 is fixedly connected to the bottom wall of the cylinder 1, and its bottom end is connected to the solenoid valve 601 to control the discharge of wastewater. During the cleaning process, after the polypeptide solid carrier and the cleaning solution are fully mixed, the wastewater is filtered through the filter plate 404 to the bottom of the cylinder 1. The operator activates the solenoid valve 601 through the control system to open the drain pipe 6, and the wastewater can then be discharged from the cylinder 1. The solenoid valve 601 can adjust the drainage time and flow rate as needed to avoid the wastewater from remaining in the cylinder 1 for a long time and to reduce the residue of the solution on the surface of the solid carrier.

[0043] refer to Figure 2 In one embodiment, the inner wall of the cylinder 1 is provided with two sets of guide grooves 5, and a guide block 501 is slidably installed on the inner wall of each guide groove 5. The two sets of guide blocks 501 are fixedly installed on the outer surface of the filter plate 404 on the side that is close to each other.

[0044] In the above embodiment, the stable movement of the filter plate 404 is ensured by the sliding engagement of the guide groove 5 and the guide block 501. Two sets of guide grooves 5 are formed on the inner wall of the cylinder 1, and a guide block 501 is slidably installed in each set of guide grooves 5. The side of the guide block 501 is fixedly connected to the outer surface of the filter plate 404. When the lifting assembly 2 drives the cleaning assembly 4 to move up and down, the filter plate 404 slides within the guide groove 5 via the guide block 501. The vertical arrangement of the guide groove 5 and the tight engagement of the guide block 501 limit the horizontal offset and tilt of the filter plate 404, ensuring that the filter plate 404 remains horizontal during lifting. When the filter plate 404 carries a large amount of solid phase carrier, it can effectively prevent tilting or jamming caused by weight or vibration.

[0045] The above description is only a preferred embodiment of the present utility model and does not limit the patent scope of the present utility model. Any equivalent structural or procedural transformations made based on the content of the present utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present utility model.

Claims

1. An automatic filling and cleaning device for polypeptide solid-phase carriers, characterized in that, Includes a cylinder (1), a lifting assembly (2) is provided through the upper end face of the cylinder (1), a filling assembly (3) is provided on one side of the cylinder (1), and a cleaning assembly (4) is provided inside the cylinder (1), the cleaning assembly (4) is connected to the driving end of the lifting assembly (2). The cleaning assembly (4) includes a motor (401) disposed inside the cylinder (1), a rotating rod (402) is fixedly installed at the output end of the motor (401), a set of paddles (403) is fixedly installed on the outer surface of the rotating rod (402), and a filter plate (404) is rotatably installed at the bottom end of the rotating rod (402) through a bearing.

2. The automatic loading and cleaning device for polypeptide solid-phase carriers according to claim 1, characterized in that, The filling component (3) includes a square tube (301) installed on the right side of the cylinder (1), the right end of the square tube (301) is fixedly connected to an adapter (302), and the output end of the adapter (302) is fixedly connected to a bend (303).

3. The automatic loading and cleaning device for polypeptide solid-phase carriers according to claim 2, characterized in that, The upper surface of the square tube (301) is fixedly connected to a nitrogen inlet pipe (306), the top end of the nitrogen inlet pipe (306) is fixedly connected to a connecting joint (304), the bottom end of the nitrogen inlet pipe (306) is fixedly connected to a perforated diverter box (305), the bottom surface of the square tube (301) is provided with an opening (307), and a filter screen (308) is fixedly installed on the inner wall of the opening (307).

4. The automatic loading and cleaning device for polypeptide solid-phase carriers according to claim 1, characterized in that, The lifting assembly (2) includes a hydraulic rod (201) installed on the upper surface of the cylinder (1), and an installation plate (202) is fixedly installed on the telescopic end of the hydraulic rod (201). The upper surface of the motor (401) is fixedly installed on the bottom surface of the installation plate (202), and a control panel (203) is fixedly installed on the upper surface of the cylinder (1).

5. The automatic loading and cleaning device for polypeptide solid-phase carriers according to claim 1, characterized in that, Two sets of support legs (101) are provided below the cylinder (1), and the upper surface of each support leg (101) is fixedly installed to the bottom surface of the cylinder (1).

6. The automatic loading and cleaning device for polypeptide solid-phase carriers according to claim 1, characterized in that, The upper surface of the cylinder (1) is provided with a feed inlet (102), and the inner wall of the feed inlet (102) is threaded with a sealing plug (103).

7. The automatic loading and cleaning device for polypeptide solid-phase carriers according to claim 1, characterized in that, The inner bottom wall of the cylinder (1) is fixedly connected to a drain pipe (6), and the bottom end of the drain pipe (6) is fixedly connected to a solenoid valve (601).

8. The automatic loading and cleaning device for polypeptide solid-phase carriers according to claim 1, characterized in that, The inner wall of the cylinder (1) is provided with two sets of guide grooves (5), and each guide groove (5) is slidably installed with a guide block (501) on its inner wall. The two sets of guide blocks (501) are fixedly installed on the outer surface of the filter plate (404) on the side that is close to each other.

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