A large tissue section embedding clamp assembly

Abstract

The utility model discloses a big organization section of tissue embedding clamping assembly relates to the technical field of embedding box, including clamping adapter seat and embedding piece, two parallel clamping surfaces are arranged in the both sides of clamping adapter seat, and the clamping surface is used for clamping on the sample clamp of automatic pathological paraffin embedding wheel type section of tissue machine, and the top of two parallel clamping surfaces is connected through a locating surface, and the end face of bottom is flush and can be in abutment with the bottom surface of sample clamp, and the embedding piece is detachably connected on the locating surface, and the embedding piece is provided with big organization section of tissue wax block. The utility model discloses utilize clamping adapter seat and the fixed connection of section of tissue machine's sample clamp, and the one -time clamping of clamping adapter seat can replace multiple embedding pieces and carry out sectioning, make big organization section of tissue wax block realize quick clamping, and can guarantee the position of rear embedding piece is relatively fixed, so repeatedly multiple times sectioning, and the section angle can all be kept on the same section surface, avoid repeatedly adjusting section angle, and the phenomenon of cutting the focus organization.

Description

Technical Field

[0001] This utility model relates to the technical field of embedding cassettes, and in particular to a large tissue section embedding clamping assembly. Background Technology

[0002] Embedding is a technique that uses embedding agents (such as organic plastics, metals, paraffin, etc.) to encapsulate powdered or bulk materials, primarily to provide physical support or chemical protection. This technique is widely used in pathology. In tissue section preparation, embedding mainly involves fixing dehydrated tissue into a hard block with paraffin, facilitating subsequent cutting into thin sections (typically 4 μm) for staining and microscopic observation.

[0003] Large tissue sections are embedded in paraffin blocks to present the entire organ or tumor and its surrounding tissues on a single pathological slide. This allows for the identification of small lesions, calculation of tumor proportions, assessment of the relationship between the tumor and surrounding tissues, and a comprehensive evaluation of tumor infiltration. Currently, paraffin blocks for embedding large tissue sections can reach lengths of 5.5 cm, widths of 4.8 cm, and thicknesses exceeding 1.5 cm (even reaching 2 cm). The paraffin blocks used for clamping alone are at least 5 mm thick, making them bulky and unsuitable for storage in conventional paraffin block storage cabinets. They require separate archiving, and their large size results in high storage costs.

[0004] Currently, when using automated paraffin embedding microtome for large pathological tissue sections, paraffin blocks are held in place by a rotating sample clamp that uses a nut to hold the paraffin block in place. Because this rotating clamping device (sample clamp) lacks a limiting structure for the paraffin block, and because existing conventional paraffin block embedding clamps are too small for large pathological tissue sections, the paraffin blocks cannot be directly and quickly held in place by the sample clamp. This results in inconsistent clamping positions each time, requiring manual adjustment of the cutting angle and multiple clamping cycles. Furthermore, repeated clamping can easily cause the paraffin block to break. If the cutting surfaces are inconsistent when the paraffin block is moved horizontally or vertically, either the tissue section is incomplete, or significant tissue may be removed to achieve a complete section. This is especially problematic when resection is required for small lesions, as the cross-sectional angle may not correspond to the previous section, leading to the removal of lesion tissue during the slicing process. Therefore, there is an urgent need to propose an embedding and clamping component for large tissue sections to achieve rapid clamping and maintain the same angle on the cutting surface during repeated slicing. Utility Model Content

[0005] The purpose of this invention is to provide a large tissue section embedding clamping component to solve the problems existing in the prior art, enabling rapid clamping of large tissue section wax blocks and maintaining the same angle on the cut surface during repeated slicing.

[0006] To achieve the above objectives, this utility model provides the following solution:

[0007] This utility model provides a large tissue section embedding clamping assembly, including a clamping adapter and an embedding slide. The clamping adapter has two parallel clamping surfaces on both sides. The clamping surfaces are used to clamp onto the sample holder of an automatic pathological paraffin embedding wheel microtome. The tops of the two parallel clamping surfaces are connected by a positioning surface, and the bottom end faces are flush and can abut against the bottom surface of the sample holder. An embedding slide is detachably connected to the positioning surface, and a large tissue section paraffin block is disposed on the embedding slide.

[0008] Preferably, the clamping adapter includes a sloping side, a stepped surface, a positioning surface, and two clamping surfaces. The positioning surface is rectangular and is sequentially connected to the sloping side, one clamping surface, the stepped surface, and the other clamping surface. The sloping side, the two clamping surfaces, the stepped surface, and the positioning surface are joined together to form a frame with a flat bottom and an open opening. The two clamping surfaces are connected to the long side of the positioning surface.

[0009] Preferably, the clamping adapter is made of metal or metal alloy; the embedded sheet is made of polyester or polypropylene.

[0010] Preferably, a slot or a block is provided on the positioning surface, and a block or slot is provided on the back of the embedded sheet, wherein the block can be inserted into the slot.

[0011] Preferably, both the insertion slot and the insertion block include a pair of strip-shaped protrusions. The cross-section of the strip-shaped protrusions is L-shaped, T-shaped, right-angled trapezoidal, or isosceles trapezoidal, and the two pairs of strip-shaped protrusions can mutually limit and clamp each other in a direction perpendicular to the strip-shaped protrusions and the positioning surface. The length direction of the strip-shaped protrusions is perpendicular to the slicing direction of the automatic pathological paraffin-embedded wheel microtome. A positioning block is provided at one end of the strip-shaped protrusion.

[0012] Preferably, a pair of strip-shaped protrusions on the positioning surface are symmetrically arranged at both ends of the positioning surface, and the distance between the two strip-shaped protrusions is not less than 1 / 3 of the length of the positioning surface; a pair of strip-shaped protrusions on the embedding sheet are symmetrically arranged at both ends of the embedding sheet and are parallel to the width direction of the embedding sheet.

[0013] Preferably, the length direction of the strip-shaped protrusion is perpendicular to the direction of the two clamping surfaces, and the height of the strip-shaped protrusion is no more than 3mm.

[0014] Preferably, the width of the clamping adapter and the embedding piece is not less than the width of the clamping adapter, the length of the embedding piece is not less than the length of the clamping adapter, and the clamping surface of the clamping adapter is located in the width direction of the clamping adapter.

[0015] Preferably, the clamping surface of the clamping adapter has a width of 5mm-8mm, and the positioning surface has a length of 4cm and a width of 2.3cm.

[0016] Preferably, the embedding slide is no more than 5cm in length, no more than 4cm in width, and no more than 2mm in thickness, and the protrusion thickness of the large tissue section wax block on the embedding slide is no more than 4mm; the embedding slide is uniformly provided with perforated holes.

[0017] The present invention achieves the following technical advantages over the prior art:

[0018] This invention utilizes a clamping adapter that is fixedly connected to the sample clamp of a microtome. The embedded slides and the clamping adapter are detachable and assembleable. With a single clamping action of the clamping adapter, multiple embedded slides can be replaced for slicing, enabling rapid clamping of large tissue sections and ensuring that the position of the embedded slides remains relatively fixed. Therefore, even after repeated slicing, the cutting angle can be maintained on the same cutting plane, avoiding the phenomenon of repeatedly adjusting the cutting angle and cutting over the lesion tissue. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of the structure of the large tissue section embedding and clamping assembly in this embodiment of the present invention. Figure 1 ;

[0021] Figure 2 This is a schematic diagram of the structure of the large tissue section embedding and clamping assembly in this embodiment of the present invention. Figure 2 ;

[0022] Figure 3 This is an exploded structural diagram of the large tissue slice embedding and clamping assembly in an embodiment of this utility model;

[0023] Figure 4 This is a schematic diagram of the embedded sheet structure in an embodiment of the present invention. Figure 1 ;

[0024] Figure 5This is a schematic diagram of the embedded sheet structure in an embodiment of the present invention. Figure 2 ;

[0025] Figure 6 This is a schematic diagram of the embedded sheet structure in an embodiment of the present invention. Figure 3 ;

[0026] Figure 7 This is a schematic diagram of the clamping adapter structure in an embodiment of the present invention. Figure 1 ;

[0027] Figure 8 This is a schematic diagram of the clamping adapter structure in an embodiment of the present invention. Figure 2 ;

[0028] In the figure: 1-clamping adapter seat, 2-positioning surface, 3-sloping side, 4-stepped surface, 5-embedded piece, 6-insertion groove, 7-insertion block, 8-hollow hole, 9-positioning stop. Detailed Implementation

[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0030] It should be noted that in the description of this utility model, the terms "upper," "lower," "left," "right," "inner," "outer," "front," "rear," "clockwise," and "counterclockwise," etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings. These are merely for ease of description and do not indicate or imply that the device or element must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," "third," and "fourth" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," "third," and "fourth" may explicitly or implicitly include one or more of the stated features. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0031] Furthermore, it should be noted that, in the description of this utility model, unless otherwise explicitly specified and limited, the terms "set," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0032] The purpose of this invention is to provide a large tissue section embedding clamping component to solve the problems existing in the prior art, enabling rapid clamping of large tissue section wax blocks and maintaining the same angle on the cut surface during repeated slicing.

[0033] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0034] Example 1

[0035] like Figures 1 to 8 As shown, this embodiment provides a large tissue section embedding clamping assembly, including a clamping adapter 2 and an embedding slide 5. The clamping adapter 2 has two parallel clamping surfaces on both sides, which are used to clamp the sample holder of an automated pathological paraffin embedding wheel microtome. The tops of the two parallel clamping surfaces are connected by a positioning surface 2, and their bottom ends are flush and can abut against the bottom surface of the sample holder. The embedding slide 5 is detachably connected to the positioning surface 2, and a large tissue section wax block is mounted on the embedding slide 5. In this embodiment, the clamping adapter 2 is fixedly connected to the sample holder of the microtome. The embedding slide 5 and the clamping adapter 2 are detachably assembled. A single clamping action of the clamping adapter 2 allows for the replacement of multiple embedding slides 5 for sectioning, enabling rapid clamping of the large tissue section wax block and ensuring that the position of the embedded slide remains relatively fixed. Therefore, even after repeated sectioning, the cutting angle can be maintained on the same cutting plane, avoiding the phenomenon of repeatedly adjusting the cutting angle and over-shaving the lesion tissue.

[0036] As an optional solution, in this embodiment, the clamping adapter 2 includes a sloping side 3, a stepped surface 4, a positioning surface 2, and two clamping surfaces. The positioning surface 2 is rectangular and is sequentially connected to the sloping side 3, one clamping surface, the stepped surface 4, and the other clamping surface. The sloping side 3, the two clamping surfaces, the stepped surface 4, and the positioning surface 2 are joined to form a frame with a flat bottom and an open opening. The two clamping surfaces are connected to the long side of the positioning surface 2. The stepped surface 4 facilitates disassembly of the clamping adapter 2 by using fingers to grip the stepped surface 4.

[0037] As an optional solution, in this embodiment, the clamping adapter 2 is made of metal or a metal alloy, which is sturdy, durable, and not easily deformed, ensuring clamping accuracy and allowing for reusability. The embedding sheet 5 is made of polyester or polypropylene; plastic is inexpensive and easy to process, making it a disposable tool for bonding with paraffin.

[0038] As an optional solution, in this embodiment, a slot 6 or a block 7 is provided on the positioning surface 2, and a block 7 or a slot 6 is provided on the back of the embedding slide 5. The block 7 can be slidably inserted into the slot 6 to achieve a detachable connection. The operation is simple, quick and convenient, and it is convenient to quickly switch between different large tissue section wax blocks.

[0039] As an optional solution, in this embodiment, both the insertion slot 6 and the insertion block 7 include a pair of strip-shaped protrusions. The cross-section of the strip-shaped protrusions is L-shaped, T-shaped, right-angled trapezoidal, or isosceles trapezoidal. Preferably, the cross-sectional dimensions of the strip-shaped protrusions are larger on the outside and smaller on the inside. They have both limiting surfaces parallel to the direction of the positioning surface 2 and limiting surfaces perpendicular to the direction of the positioning surface 2, which can prevent the embedded slide 5 from moving during the slicing process. The two pairs of strip-shaped protrusions can mutually limit and clamp each other in the direction perpendicular to the strip-shaped protrusions and the positioning surface 2. A positioning block 9 is provided at one end of the strip-shaped protrusion, which makes it easy for the embedded slide 5 to slide into the strip-shaped protrusion from one end along the length direction of the strip-shaped protrusion and be positioned by the positioning block 9 at the other end to prevent the position from being too off-center. At the same time, it can limit the embedded slide 5. The length direction of the strip-shaped protrusions is perpendicular to the slicing direction of the automatic pathological paraffin embedding wheel microtome. When in use, the strip-shaped protrusions are horizontal.

[0040] As an optional solution, in this embodiment, a pair of strip-shaped protrusions on the positioning surface 2 are symmetrically arranged at both ends of the positioning surface 2, and the distance between the two strip-shaped protrusions is not less than 1 / 3 of the length of the positioning surface 2, so that the force is uniform during the slicing process of the embedded slide 5. The pair of strip-shaped protrusions on the embedded slide 5 are symmetrically arranged at both ends of the embedded slide 5 and are parallel to the wide side direction of the embedded slide 5, ensuring that they are perpendicular to the slicing direction of the automatic pathological paraffin embedding wheel microtome.

[0041] As an alternative, in this embodiment, the length direction of the strip protrusion is perpendicular to the direction of the two clamping surfaces, and the height of the strip protrusion is no more than 3mm, which occupies a small volume and can be staggered to save storage space.

[0042] As an optional solution, in this embodiment, the width of the clamping adapter 2 and the embedding piece 5 is not less than the width of the clamping adapter 2, the length of the embedding piece 5 is not less than the length of the clamping adapter 2, and the clamping surface of the clamping adapter 2 is located in the width direction of the clamping adapter.

[0043] As an alternative, in this embodiment, the clamping surface of the clamping adapter 2 is 5mm-8mm wide, the positioning surface 2 is 4cm long and 2.3cm wide, and its volume and shape are similar to existing small tissue section embedding blocks. It can be clamped directly without changing the sample clamp.

[0044] As an optional solution, in this embodiment, the length of the embedding slide 5 does not exceed 5cm, the width does not exceed 4cm, and the thickness does not exceed 2mm. The protruding thickness of the large tissue section wax block on the embedding slide 5 is no greater than 4mm, so that the thickness of the large tissue section embedding wax block plus the strip protrusion does not exceed 8mm, and the overall thickness is much smaller than that of existing embedding wax blocks. The embedding slide 5 is uniformly provided with perforated holes 8 to ensure that the paraffin embedding has sufficient connection surface and support force, so that the wax block does not break during sectioning.

[0045] Example 2

[0046] This embodiment provides a method for using a large tissue slice embedding and clamping component, as detailed below:

[0047] First, clamp and fix the positioning surface 2 of the clamping adapter 2 onto the sample holder of the automatic pathology paraffin embedding wheel microtome with the positioning surface 2 facing upwards. Then, insert the insertion block 7 of the embedding slide 5, which carries the large tissue section paraffin block, laterally into the insertion slot 6 of the clamping adapter 2 from the side. The assembly and positioning process of the embedding slide 5 only takes 2 seconds, and then the automatic pathology paraffin embedding wheel microtome can be turned on for sectioning. After the large tissue paraffin section is completed, the embedding slide 5 with the large tissue section paraffin block can be directly removed for archiving and storage. Since the clamping adapter 2 is not used for storage, the storage volume is much smaller, which facilitates long-term storage and reduces storage costs.

[0048] The entire operation process in this embodiment is simple and quick, without the need for multiple clamping and adjustment of the cutting angle by the sample clamp. This allows for rapid clamping of large tissue section paraffin blocks and ensures that the position of the embedded section 5 is relatively fixed. Therefore, even after repeated slicing, the cutting angle can be kept on the same cutting plane, avoiding the phenomenon of repeatedly adjusting the cutting angle and cutting over the lesion tissue.

[0049] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "this embodiment," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with the described embodiment or example, which are included in at least one embodiment or example of this utility model. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0050] This utility model uses specific examples to illustrate its principles and implementation methods. The above description of the embodiments is only for the purpose of helping to understand the method and core idea of ​​this utility model. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the idea of ​​this utility model. In summary, the content of this specification should not be construed as a limitation of this utility model.

Claims

1. A large tissue section embedding clamping assembly, characterized in that: The device includes a clamping adapter and an embedding slide. The clamping adapter has two parallel clamping surfaces on both sides. The clamping surfaces are used to clamp onto the sample holder of an automatic pathological paraffin embedding wheel microtome. The tops of the two parallel clamping surfaces are connected by a positioning surface, and the bottom end faces are flush and can abut against the bottom surface of the sample holder. An embedding slide is detachably connected to the positioning surface, and a large tissue section paraffin block is placed on the embedding slide.

2. The large tissue section embedding and clamping assembly according to claim 1, characterized in that: The clamping adapter includes a sloping side, a stepped surface, a positioning surface, and two clamping surfaces. The positioning surface is rectangular and is sequentially connected to the sloping side, one clamping surface, the stepped surface, and the other clamping surface. The sloping side, the two clamping surfaces, the stepped surface, and the positioning surface are joined together to form a frame with a flat bottom and an open opening. The two clamping surfaces are connected to the long side of the positioning surface.

3. The large tissue section embedding clamping assembly according to claim 1, characterized in that: The clamping adapter is made of metal or metal alloy; the embedding sheet is made of polyester or polypropylene.

4. The large tissue section embedding and clamping assembly according to claim 1, characterized in that: A slot or a block is provided on the positioning surface, and a block or slot is provided on the back of the embedded sheet, wherein the block can be inserted into the slot.

5. The large tissue section embedding and clamping assembly according to claim 4, characterized in that: Both the insertion slot and the insertion block include a pair of strip-shaped protrusions. The cross-section of the strip-shaped protrusions is L-shaped, T-shaped, right-angled trapezoidal, or isosceles trapezoidal, and the two pairs of strip-shaped protrusions can mutually limit and clamp each other in a direction perpendicular to the strip-shaped protrusions and the positioning surface. The length direction of the strip-shaped protrusions is perpendicular to the slicing direction of the automatic pathological paraffin-embedded wheel microtome. A positioning stop is provided at one end of the strip-shaped protrusion.

6. The large tissue section embedding clamping assembly according to claim 5, characterized in that: A pair of strip-shaped protrusions are symmetrically arranged at both ends of the positioning surface, and the distance between the two strip-shaped protrusions is not less than 1 / 3 of the length of the positioning surface; a pair of strip-shaped protrusions are symmetrically arranged at both ends of the embedding sheet, and are parallel to the width direction of the embedding sheet.

7. The large tissue section embedding clamping assembly according to claim 5, characterized in that: The length direction of the strip-shaped protrusion is perpendicular to the direction of the two clamping surfaces, and the height of the strip-shaped protrusion is no more than 3mm.

8. The large tissue section embedding clamping assembly according to claim 1, characterized in that: The width of the clamping adapter and the embedding piece is not less than the width of the clamping adapter, the length of the embedding piece is not less than the length of the clamping adapter, and the clamping surface of the clamping adapter is located in the width direction of the clamping adapter.

9. The large tissue section embedding clamping assembly according to claim 1, characterized in that: The clamping surface of the clamping adapter has a width of 5mm-8mm, and the positioning surface has a length of 4cm and a width of 2.3cm.

10. The large tissue section embedding clamping assembly according to claim 1, characterized in that: The embedding slide is no more than 5cm in length, no more than 4cm in width, and no more than 2mm in thickness. The protrusion thickness of the large tissue section wax block on the embedding slide is no more than 4mm. The embedding slide is uniformly provided with perforated holes.

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