A fluorescent temperature detection system

Abstract

The utility model provides a fluorescent temperature detection system, the utility model discloses in order to solve the problem that the measurement precision and stability of existing fluorescent temperature sensing device have to be improved, the utility model fluorescent temperature detection system is provided with laser, rare earth fluorescent sample and first lens in the fluorescence generation chamber, is provided with two groups of lens - filter assembly in the fluorescence detection chamber, the filter wavelength is different in two groups of lens - filter assembly through the filter, is provided with two fluorescent detectors on the fluorescent detection circuit, and the emission light of rare earth fluorescent sample is irradiated on the half -transparent half -mirror through first lens, and the reflection light of half -transparent half -mirror is incident into one fluorescent detector through first lens - filter assembly, the light splitting of passing through half -transparent half -mirror is incident to full -mirror, and the reflection light of full -mirror is incident into another fluorescent detector through second lens - filter assembly, the utility model fluorescent temperature sensing optical system's light path coupling structure is simple, and the stability and precision of temperature measurement are improved through the characteristic of rare earth fluorescence.

Description

Technical Field

[0001] This utility model relates to a fluorescence temperature sensing device. Background Technology

[0002] Fluorescence thermometry is an optical temperature measurement technique based on the luminescence properties of fluorescent materials. It relies on the instantaneous optical response of fluorescent materials to temperature changes (such as rapid modulation of fluorescence lifetime, intensity, or wavelength), combined with high-speed photodetectors and lock-in amplification technology, to achieve real-time temperature monitoring at the microsecond to nanosecond level. Some rare-earth-doped phosphors exhibit excellent linearity between fluorescence parameters and temperature, with a sensitivity down to 0.01 K. Accurate analysis of the luminescence signal using a spectrometer allows for stable temperature measurement accuracy within ±0.1 K, maintaining high resolution even at the nanoscale, such as for single cells or micro / nano devices. FIR thermometry relies on the ratio of fluorescence signal intensities at two wavelengths, the ratio of luminescence intensities at two excited-state energy levels, rather than the absolute intensity of a single wavelength. Ambient light (such as natural light or LED backlight) is typically a broadband light source, exhibiting similar interference to both wavelengths. Therefore, ratio calculations effectively cancel common-mode interference, significantly reducing the impact of background light fluctuations on the measurement results, resulting in strong anti-interference capabilities. It is widely used in medical hyperthermia, and its unique properties make it an important supplement to traditional temperature measurement techniques. Utility Model Content

[0003] The purpose of this invention is to address the problem that the measurement accuracy and stability of existing fluorescence temperature sensing devices need to be improved, and to provide a fluorescence temperature detection system.

[0004] This utility model of fluorescence temperature detection system includes a fluorescence generation chamber, a fluorescence detection chamber, a laser, a rare earth fluorescent sample, a first lens, a semi-transparent and semi-reflective mirror, a total reflection mirror, two sets of lens-filter assemblies, a fluorescence detection circuit and a signal processing circuit. The fluorescence generation chamber and the fluorescence detection chamber are connected by an optical path through hole. The first lens is set in the fluorescence generation chamber, and the laser generated by the laser irradiates the rare earth fluorescent sample.

[0005] The fluorescence detection chamber is equipped with a semi-transparent and semi-reflective mirror, a total reflection mirror, two sets of lens-filter components, a fluorescence detection circuit, and a signal processing circuit. Each set of lens-filter components has a first lens, a filter, and a second lens arranged sequentially along the optical path transmission direction. The filtering wavelengths of the two sets of lens-filter components are different.

[0006] Two fluorescence detectors are set on the fluorescence detection circuit. The emitted light of the rare earth fluorescent sample is irradiated onto the semi-transparent mirror through the first lens. The reflected light from the semi-transparent mirror passes through the first lens-filter assembly and enters the fluorescence detector on the fluorescence detection circuit. The light is then split by the semi-transparent mirror and enters the total reflection mirror. The reflected light from the total reflection mirror passes through the second lens-filter assembly and enters the other fluorescence detector on the fluorescence detection circuit. The fluorescence detector is electrically connected to the signal processing circuit.

[0007] This novel fluorescence temperature detection system mainly comprises a fluorescence generation chamber and a fluorescence detection chamber. In the fluorescence generation chamber, a laser emitted by a laser excites a rare-earth fluorescent sample to emit light. The emitted fluorescence is focused by a first lens and first reaches a semi-transparent mirror. Part of the light is reflected, and the reflected light passes through the first lens, then through a (bandpass) filter to obtain light of a specific wavelength. Finally, it passes through a second lens, and fluorescence A reaches the fluorescence detector. The other part of the light is transmitted, reflected by a total reflection mirror, and then filtered by a second lens-filter assembly, and fluorescence B reaches another fluorescence detector. Both fluorescence detectors are driven and controlled by the same light source, simultaneously detecting fluorescence A and fluorescence B signals. Fluorescence A and fluorescence B pass through different filters, resulting in different wavelengths of filtered light and different intensities at different wavelengths. Based on the particle number distribution pattern, fluorescence intensity is found to be related to temperature. However, many factors influence fluorescence intensity, making accurate temperature measurement difficult. By using the ratio of fluorescence intensities at two energy levels, environmental interference can be eliminated, thus obtaining the relationship between the fluorescence intensity ratio and temperature, and using the fluorescence intensity ratio to measure temperature. Finally, the detected fluorescence signal is transmitted to the signal demodulation processing circuit, which calculates the fluorescence intensity ratio and displays the temperature.

[0008] The fluorescence temperature sensing optical system provided by this invention facilitates installation by using a simplified optical path coupling device, while also improving the system's stability and measurement accuracy through the characteristics of rare-earth fluorescence.

[0009] The fluorescence temperature sensing optical system of this utility model also includes a fluorescence detection circuit, which converts the fluorescence signal measured by the fluorescence detector into an electrical signal; a signal demodulation processing circuit, which receives the electrical signal sent by the fluorescence detection circuit and extracts fluorescence intensity information from the electrical signal; and a display device, which receives the data signal sent by the signal demodulation processing circuit, performs data analysis by comparing it with existing spectra, and displays it on the display screen.

[0010] This invention relates to a light source driving circuit that controls the pulse width and amplitude of the light signal emitted by a fluorescent excitation light source through a light source driving signal. After the fluorescence detector converts the light signal into an electrical signal, the fluorescence signal detection circuit receives the electrical signal sent by the fluorescence detector and filters and amplifies it. The signal demodulation processing circuit performs digital signal processing on the electrical signal processed by the fluorescence signal detection circuit to obtain temperature information data. Based on this temperature information data, it sends a control signal to the light source driving circuit, enabling the light source driving circuit to adjust the pulse width and amplitude of the light signal emitted by the fluorescent excitation light source. After the signal demodulation processing circuit converts the analog-to-digital signal to obtain the temperature information, it displays the temperature information on a display device, allowing the testing personnel to intuitively obtain relevant information about the detected temperature. Attached Figure Description

[0011] Figure 1 This is a schematic diagram of the overall structure of the fluorescent temperature sensing device of this utility model. The bold line represents the optical path. Detailed Implementation

[0012] Specific Implementation Method 1: The fluorescence temperature detection system in this implementation method includes a fluorescence generation chamber, a fluorescence detection chamber, a laser 1, a rare earth fluorescent sample 2, a first lens 3, a semi-transparent and semi-reflective mirror 6, a total reflection mirror 7, two sets of lens-filter assemblies, a fluorescence detection circuit 12, and a signal processing circuit 13. The fluorescence generation chamber and the fluorescence detection chamber are connected through an optical path through hole. The laser 1, the rare earth fluorescent sample 2, and the first lens 3 are arranged in the fluorescence generation chamber. The laser generated by the laser 1 irradiates the rare earth fluorescent sample 2.

[0013] The fluorescence detection chamber is equipped with a semi-transparent mirror 6, a total reflection mirror 7, two sets of lens-filter components, a fluorescence detection circuit 12, and a signal processing circuit 13. Each set of lens-filter components is provided with a first lens 8, a filter 9, and a second lens 10 in sequence along the optical path transmission direction. The filtering wavelengths of the two sets of lens-filter components through the filter 9 are different.

[0014] Two fluorescence detectors 11 are provided on the fluorescence detection circuit 12. The emitted light of the rare earth fluorescent sample 2 is irradiated onto the semi-transparent mirror 6 through the first lens 3. The reflected light from the semi-transparent mirror 6 passes through the first lens-filter assembly and enters the fluorescence detector 11 on the fluorescence detection circuit 12. The light is then split by the semi-transparent mirror 6 and enters the total reflection mirror 7. The reflected light from the total reflection mirror 7 passes through the second lens-filter assembly and enters the other fluorescence detector 11 on the fluorescence detection circuit 12. The fluorescence detector 11 is electrically connected to the signal processing circuit 13.

[0015] Specific Implementation Method Two: The difference between this implementation method and Specific Implementation Method One is that a switch button is provided on the laser 1.

[0016] Specific Implementation Method 3: This implementation method differs from Specific Implementation Method 1 or 2 in that a cooling device 4 is also provided in the fluorescence generation chamber.

[0017] In this embodiment, the rare earth fluorescent sample is cooled by cooling device 4.

[0018] Specific Implementation Method Four: This implementation method differs from one of the specific implementation methods one to three in that the first lens 8, filter 9, and second lens 10 in the lens-filter assembly are fixed on the substrate 15.

[0019] Specific Implementation Method 5: This implementation method differs from Specific Implementation Methods 1 to 4 in that the signal processing circuit 13 is electrically connected to the display screen 14, and the display screen 14 is located at the bottom of the fluorescence detection chamber.

[0020] Example: The fluorescence temperature detection system in this example includes a fluorescence generation chamber, a fluorescence detection chamber, a laser 1, a rare earth fluorescent sample 2, a first lens 3, a semi-transparent mirror 6, a total reflection mirror 7, two sets of lens-filter assemblies, a fluorescence detection circuit 12, and a signal processing circuit 13. The fluorescence generation chamber and the fluorescence detection chamber are connected by an optical path through-hole. The fluorescence generation chamber is equipped with a laser 1, a rare earth fluorescent sample 2, a first lens 3, a cooling device 4, and a power supply 5. The laser generated by the laser 1 irradiates the rare earth fluorescent sample 2. The power supply 5 is located in the fluorescence generation chamber and supplies power to the cooling device 4 and the laser 1. The cooling device 4 is a fan.

[0021] The fluorescence detection chamber is equipped with a semi-transparent mirror 6, a total reflection mirror 7, two sets of lens-filter components, a fluorescence detection circuit 12, and a signal processing circuit 13. Each set of lens-filter components is provided with a first lens 8, a filter 9, and a second lens 10 in sequence along the optical path transmission direction. The filters 9 in the two sets of lens-filter components are different, and the filtering wavelengths of the two sets of lens-filter components through the filters 9 are different.

[0022] Two fluorescence detectors 11 are provided on the fluorescence detection circuit 12. The emitted light of the rare earth fluorescent sample 2 is irradiated onto the semi-transparent mirror 6 through the optical path through the first lens 3. The reflected light from the semi-transparent mirror 6 is incident into the fluorescence detector 11 on the fluorescence detection circuit 12 through the first lens-filter assembly. After being split by the semi-transparent mirror 6, the light is incident onto the total reflection mirror 7. The reflected light from the total reflection mirror 7 is incident into the other fluorescence detector 11 on the fluorescence detection circuit 12 through the second lens-filter assembly. The fluorescence detector 11 is electrically connected to the signal processing circuit 13, and the signal processing circuit 13 is electrically connected to the display screen 14. The display screen 14 is located at the bottom of the fluorescence detection chamber.

[0023] In this embodiment, a controller 16 is provided in the fluorescence generation chamber. The controller 16 is used to control the cooling device 4 and the fluorescence detection circuit 12. During the temperature measurement process, the laser 1 is turned on and the fluorescence detection circuit 12 is working. After the temperature measurement is completed, the laser 1 is turned off and the controller 16 controls the cooling device 4 to start cooling the rare earth fluorescent sample 2.

[0024] The temperature measurement process using the fluorescence temperature detection system in this embodiment is as follows:

[0025] A laser excites a rare-earth fluorescent sample to produce fluorescence. The fluorescence is focused by lens 3 and illuminates a semi-transparent mirror 6. The reflected light is then focused and filtered by a first lens-filter assembly to extract fluorescence A. The intensity of fluorescence A is detected by a fluorescence detector 11. Another portion of the transmitted light passes through the semi-transparent mirror 6 and reaches a total reflection mirror 7. The reflected light is focused and filtered by a second lens-filter assembly to extract fluorescence B. The intensities of fluorescence A and fluorescence B are then acquired by two fluorescence detectors 11, respectively. Finally, the data processing circuit calculates the temperature corresponding to the intensity ratio of fluorescence A and fluorescence B, and displays the result on a display screen 14.

[0026] This novel fluorescence temperature detection system utilizes fluorescence thermometry, which offers high precision. By measuring the temperature through the relationship between the fluorescence intensity ratio of two energy levels and the temperature, environmental interference is eliminated, making fluorescence thermometry more accurate and stable.

Claims

1. A fluorescence temperature detection system, characterized in that... The fluorescence temperature detection system includes a fluorescence generation chamber, a fluorescence detection chamber, a laser (1), a rare earth fluorescent sample (2), a first lens (3), a semi-transparent mirror (6), a total reflection mirror (7), two sets of lens-filter assemblies, a fluorescence detection circuit (12), and a signal processing circuit (13). The fluorescence generation chamber and the fluorescence detection chamber are connected by an optical path through-hole. The laser (1), the rare earth fluorescent sample (2), and the first lens (3) are set in the fluorescence generation chamber. The laser generated by the laser (1) irradiates the rare earth fluorescent sample (2). The fluorescence detection chamber is equipped with a semi-transparent mirror (6), a total reflection mirror (7), two sets of lens-filter components, a fluorescence detection circuit (12) and a signal processing circuit (13). Each set of lens-filter components is provided with a first lens (8), a filter (9) and a second lens (10) in sequence along the optical path transmission direction. The filtering wavelengths of the two sets of lens-filter components through the filter (9) are different. Two fluorescence detectors (11) are set on the fluorescence detection circuit (12). The emitted light of the rare earth fluorescent sample (2) is irradiated on the semi-transparent mirror (6) through the first lens (3). The reflected light of the semi-transparent mirror (6) is incident into the fluorescence detector (11) on the fluorescence detection circuit (12) through the first lens-filter assembly. The light is split by the semi-transparent mirror (6) and incident on the total reflection mirror (7). The reflected light of the total reflection mirror (7) is incident into the other fluorescence detector (11) on the fluorescence detection circuit (12) through the second lens-filter assembly. The fluorescence detector (11) is electrically connected to the signal processing circuit (13).

2. The fluorescence temperature detection system according to claim 1, characterized in that... A switch button is provided on the laser (1).

3. The fluorescence temperature detection system according to claim 1, characterized in that... A cooling device (4) is also installed in the fluorescence generation chamber.

4. The fluorescence temperature detection system according to claim 1, characterized in that... The first lens (8), the filter (9), and the second lens (10) in the lens-filter assembly are fixed on the substrate (15).

5. The fluorescence temperature detection system according to claim 1, characterized in that... The signal processing circuit (13) is electrically connected to the display screen (14), which is located at the bottom of the fluorescence detection chamber.

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