An experimental device for microbial strain proportioning

Abstract

The utility model relates to microorganism strain proportioning technical field discloses a kind of experimental devices for microorganism strain proportioning, including jar body, installation position is opened in jar body;Mixed component, mixed component is set in jar body, and mixed component is used to proportion and mix to microorganism strain, and mixed component includes driving motor, movable frame, auger shaft and fixed block, driving motor is fixedly connected with jar body, movable frame is set in jar body, movable frame is drivingly connected with driving motor, auger shaft array is set in jar body, each auger shaft is drivingly connected with driving motor, multiple fluid effects are formed by the cooperation of mixed component internal parts, under the cooperation of multiple fluid effects, avoid in stirring Large amount of foam covers liquid surface to hinder oxygen dissolving, lead to aerobic bacteria growth is limited;Foam layer hinders liquid flow, causes local strain or nutrient concentration difference;Foam occupies reactor space, reduces actual utilization rate and other problems.

Description

Technical Field

[0001] This utility model belongs to the field of microbial strain ratio technology, specifically, it relates to an experimental device for microbial strain ratio. Background Technology

[0002] Microbial culture is a method of propagating microorganisms under artificial conditions. Depending on the type of microorganism and its requirements for environmental conditions such as nutrients, temperature, oxygen, moisture, and pH, and in conjunction with specific production and experimental requirements, there are different culture methods. These can be divided into two categories: aerobic culture and anaerobic culture. Submerged culture is suitable for large-scale fermentation culture of aerobic microorganisms. In a large volume of liquid culture medium, sterile air is introduced and continuously stirred, allowing the microorganisms to fully contact the air, rapidly multiply, and accumulate metabolic products. Microbial strain cultivation devices are the main equipment for microbial cultivation.

[0003] A document with publication number (CN221759839U) discloses a microbial strain cultivation device, including a cultivation tank and a bidirectional drive mechanism. The cultivation tank has a rotating cylinder rotatably connected to it via a bearing, and a rotating column is rotatably connected inside the rotating cylinder. A drive chamber is located at the upper end of the cultivation tank. The upper end of the rotating column is rotatably connected to the top wall of the drive chamber via a bearing. A connecting column is fixedly connected to the lower end of the rotating column. Evenly distributed stirring rods are fixedly connected to both the connecting column and the middle of the rotating cylinder, and evenly distributed stirring blades are fixedly connected to the middle of each stirring rod. The bidirectional drive mechanism is located inside the drive chamber. The upper ends of the rotating column and the rotating cylinder are fixedly connected to the middle of the bidirectional drive mechanism. This microbial strain cultivation device can perform bidirectional stirring of the microbial culture medium in two layers, effectively improving the mixing efficiency of the microbial strain and the microbial culture medium, thereby effectively improving the microbial strain cultivation efficiency.

[0004] The above-mentioned device mixes the biological culture medium inside the device by double-layer rotation of multiple stirring shafts. However, during the mixing of microbial strains and microbial culture medium, a large amount of foam is generated due to stirring. The foam covers the liquid surface and hinders oxygen dissolution, which restricts the growth of aerobic bacteria. The foam layer hinders the flow of liquid, causing local differences in bacterial or nutrient concentrations. The foam occupies reactor space and reduces the actual utilization rate.

[0005] In view of this, this utility model is proposed. Utility Model Content

[0006] To solve the technical problem of foam elimination in microbial strain mixing, the basic concept of the technical solution adopted by this utility model is as follows:

[0007] An experimental apparatus for the proportioning of microbial strains includes a tank with an installation position inside the tank; a mixing component disposed inside the tank, the mixing component being used for the proportioning and mixing of microbial strains, the mixing component including a drive motor, a movable frame, an auger shaft, and fixed blocks, the drive motor being fixedly connected to the tank, the movable frame being disposed inside the tank and being drivenly connected to the drive motor, an array of auger shafts being disposed inside the tank, each auger shaft being drivenly connected to the drive motor, and fixed blocks being symmetrically disposed inside the tank, each fixed block being drivenly connected to the drive motor.

[0008] In a preferred embodiment of the present invention, the output shaft of the drive motor is fixedly connected to a drive shaft, the drive shaft is rotatably connected to the tank body, and first connecting rods are arranged in an array and symmetrically on the drive shaft. Secondary rods are also arranged in an array on the drive shaft, and each first connecting rod and secondary rod is fixedly connected to the drive shaft.

[0009] In a preferred embodiment of this utility model, each of the opposite first connecting rods is fixedly connected to a corresponding auger shaft, and the auger shafts face opposite directions.

[0010] In a preferred embodiment of this utility model, a support rod is fixedly connected between each corresponding first connecting rod and the secondary rod, a fixing block is arranged in an array on each support rod, a stirring group is symmetrically arranged on each fixing block, and an end block is fixedly connected to the end of each stirring group.

[0011] In a preferred embodiment of the present invention, each of the stirring groups consists of two symmetrical side plates, and multiple diversion blocks are fixedly connected between the side plates, with each diversion block forming a hollow tooth-like structure.

[0012] In a preferred embodiment of the present invention, a second abutting block is fixedly connected to the upper end of each of the first connecting rods, each of the second abutting blocks abuts against a first abutting block, and each of the first abutting blocks is fixedly connected to the movable frame.

[0013] In a preferred embodiment of this utility model, the inner wall of the tank is fixedly connected to an installation frame, and multiple springs are provided between the movable frame and the installation frame. The end of each spring is fixedly connected to the movable frame and the installation frame respectively, and the movable frame and the installation frame are slidably connected.

[0014] In a preferred embodiment of this utility model, the movable frame is symmetrically provided with support rods, each support rod is fixedly connected to the movable frame, and each support rod is provided with an array of ring blocks, each ring block being fixedly connected to the corresponding support rod.

[0015] In a preferred embodiment of the present invention, each of the ring blocks is ring-shaped and downward-pointingly conical, and each ring block is in close contact with the inner wall of the tank.

[0016] In a preferred embodiment of the present invention, the upper end of the tank is provided with a conveying end, which is connected to the tank by fasteners, and the bottom end of the tank is fixedly connected with a discharge end.

[0017] Compared with the prior art, the present invention has the following advantages:

[0018] 1. This experimental apparatus for microbial strain formulation utilizes a toothed structure in the flow divider to generate multi-stage shear effects, segmenting the liquid flow into fine micro-clusters. The sharp edges formed by adjacent teeth create local high-pressure gradients during rotation, significantly enhancing the turbulent kinetic energy dissipation rate and achieving efficient mixing. The symmetrical side plates of the stirring group form Venturi-type flow channels, which, combined with the irregular gaps between the teeth, induce counter-rotating Taylor-Green vortex pairs. This design shortens the mixing time and generates a high shear rate, effectively depolymerizing the microbial strains. The hollowed-out teeth of the flow divider generate periodic pressure pulsations during rotation, and the radius of curvature at the tooth tips generates a high Laplace pressure difference. The forced vibration acceleration at the gas-liquid interface reaches a high order of magnitude, disrupting the elasticity of the foam film. Jet streams are generated between the teeth, and the impact force of these jets eliminates the foam. The microchannels formed by the tooth gaps generate Dean vortices, increasing bubble migration speed and surface renewal frequency, thus reducing the foam phase content. This prevents the generation of large amounts of foam covering the liquid surface during stirring, which hinders oxygen dissolution and restricts the growth of aerobic bacteria. The foam layer also impedes liquid flow.

[0019] 2. In this experimental apparatus for the proportioning of microbial strains, the first connecting rod drives the auger shaft in a circular motion trajectory during rotation. Compared with horizontal and vertical straight stirring components, the spiral setting of the auger shaft increases the shear and extrusion forces on the microbial strains during movement. Furthermore, the spiral setting forms multi-layered vortices. The generation of multi-layered vortices leads to the collision between the vortices. By mixing the microbial strains through the collision of multi-layered vortices, the concentration difference between the microbial strains is kept the same, and the mixing efficiency of this apparatus is improved, so as to fully mix the microbial strains.

[0020] 3. In this experimental device for microbial strain proportioning, the first abutting block pushes the movable frame to rise and fall intermittently. During the intermittent rise and fall of the movable frame, the spring is intermittently compressed, and the spring intermittently deforms and applies the deformation force to the movable frame. The movable frame drives the support rod, and the support rod drives multiple ring blocks to rise and fall continuously. During the rise and fall, the ring blocks rub against the inner wall of the tank, scraping off the microbial strains adhering to the inner wall of the tank downwards. Furthermore, during the continuous rise and fall, due to the ring shape and downward conical structure of the ring blocks, the fluid in the tank is divided into multiple vortices and the direction of the vortices is guided downwards, thereby improving the mixing efficiency.

[0021] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings. Attached Figure Description

[0022] In the attached diagram:

[0023] Figure 1 This is a schematic diagram of the present invention;

[0024] Figure 2 This is a schematic diagram of the present invention;

[0025] Figure 3 This is a schematic diagram of the present invention;

[0026] Figure 4 This is a schematic diagram of the present invention;

[0027] Figure 5 This is a schematic diagram of the present invention.

[0028] In the diagram: 1. Tank body; 11. Conveying end; 12. Discharging end; 2. Drive motor; 21. Drive shaft; 3. Movable frame; 31. Mounting frame; 32. Spring; 33. First abutting block; 34. First connecting rod; 35. Second abutting block; 36. Support rod; 37. Ring block; 38. Screw shaft; 4. Secondary rod; 41. Support rod; 42. Fixing block; 43. Mixing group; 44. Diverting block; 45. End block. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate this utility model.

[0030] Please see Figure 1-5 An experimental apparatus for the proportioning of microbial strains includes a tank 1 with an installation position inside; a mixing component disposed inside the tank 1, used for the proportioning and mixing of microbial strains; the mixing component includes a drive motor 2, a movable frame 3, an auger shaft 38, and fixed blocks 42; the drive motor 2 is fixedly connected to the tank 1; the movable frame 3 is disposed inside the tank 1 and is driven by the drive motor 2; an array of auger shafts 38 is disposed inside the tank 1, each auger shaft 38 being driven by the drive motor 2; and fixed blocks 42 are symmetrically disposed inside the tank 1, each fixed block 42 being driven by the drive motor 2. Through the cooperation of the internal components of the mixing component, a multiple fluid effect is formed. This multiple fluid effect avoids problems such as excessive foam covering the liquid surface during stirring, hindering oxygen dissolution and limiting the growth of aerobic bacteria; foam layer obstructing liquid flow, causing local differences in bacterial or nutrient concentrations; and foam occupying reactor space, reducing actual utilization.

[0031] The output shaft of the drive motor 2 is fixedly connected to the drive shaft 21, which is rotatably connected to the tank body 1. First connecting rods 34 are arranged in an array and symmetrically on the drive shaft 21. Secondary rods 4 are also arranged in an array on the drive shaft 21. Each first connecting rod 34 and secondary rod 4 is fixedly connected to the drive shaft 21. Each opposing first connecting rod 34 is fixedly connected to a corresponding auger shaft 38. Each auger shaft 38 faces opposite directions. The drive motor 2 drives the drive shaft 21 to rotate via its output shaft, and the drive shaft 21, during rotation, drives the corresponding first connecting rods 34. When the auxiliary rod 4 rotates, the first connecting rod 34 drives the auger shaft 38 to move in a circular trajectory. Compared with horizontal and vertical straight stirring components, the spiral setting of the auger shaft 38 increases the shearing and extrusion forces on the microbial strains during movement. Furthermore, the spiral setting forms multi-layer vortices. The generation of multi-layer vortices leads to the collision between the vortices. By mixing the microbial strains through the collision of multi-layer vortices, the concentration difference between the microbial strains is kept the same, and the mixing efficiency of this device is improved, so as to fully mix the microbial strains.

[0032] Each corresponding first connecting rod 34 and secondary rod 4 is fixedly connected to a support rod 41. Each support rod 41 is arranged with an array of fixing blocks 42. Each fixing block 42 is symmetrically arranged with a stirring group 43. Each stirring group 43 is fixedly connected to an end block 45. Each stirring group 43 consists of two symmetrical side plates. Multiple diversion blocks 44 are fixedly connected between the side plates. Each diversion block 44 forms a hollow tooth-like structure. The first connecting rod 34 and secondary rod 4 are driven by the rotating drive shaft 21. The first connecting rod 34 and secondary rod 4 drive the support rod 41. The support rod 41 drives the fixing blocks 42. The fixing blocks 42 drive the stirring group 43 and diversion blocks 44 to rotate and come into contact with the microbial strains. The tooth-like structure of the diversion blocks 44 generates a multi-level shearing effect, dividing the liquid flow into small micro-clusters. The sharp edges formed by adjacent teeth form local angular edges when rotating. The high-pressure gradient significantly enhances the turbulent kinetic energy dissipation rate, achieving efficient mixing. The symmetrical side plates of the stirring group 43 form a Venturi-type flow channel, which, combined with the irregular gaps between the teeth, induces counter-rotating Taylor-Green vortex pairs. This design shortens the mixing time and generates a high shear rate, effectively depolymerizing microbial strains. The hollow teeth of the flow divider block 44 generate periodic pressure pulsations during rotation, and the radius of curvature of the tooth tips generates a high Laplace pressure difference. The forced vibration acceleration of the gas-liquid interface reaches a high order of magnitude, destroying the elasticity of the foam film. Jet streams are generated between the teeth, and the impact force generated by the jets impacts and eliminates the foam. The microchannels formed by the gaps between the teeth generate Dean vortices, increasing the bubble migration speed and surface renewal frequency, thus reducing the foam phase content. This avoids the generation of a large amount of foam covering the liquid surface during stirring, which hinders oxygen dissolution and restricts the growth of aerobic bacteria. The foam layer also hinders liquid flow.

[0033] Each of the first connecting rods 34 has a second abutting block 35 fixedly connected to its upper end, and each second abutting block 35 abuts against a first abutting block 33. Each first abutting block 33 is fixedly connected to the movable frame 3. An installation frame 31 is fixedly connected to the inner wall of the tank body 1. Multiple springs 32 are provided between the movable frame 3 and the installation frame 31. The end of each spring 32 is fixedly connected to both the movable frame 3 and the installation frame 31. The movable frame 3 and the installation frame 31 are slidably connected. Support rods 36 are symmetrically arranged on the movable frame 3. Each support rod 36 is fixedly connected to the movable frame 3. Each support rod 36 has an array of ring blocks 37. Each ring block 37 is fixedly connected to the corresponding support rod 36. Each ring block 37 is ring-shaped and has a downward cone shape. All blocks 37 are in close contact with the inner wall of the tank 1. The first connecting rod 34 drives the second abutting block 35 to abut against the first abutting block 33 during rotation. During the abutting, intermittent transmission occurs. The first abutting block 33 pushes the movable frame 3 to rise and fall intermittently. During the intermittent rise and fall of the movable frame 3, the spring 32 is intermittently compressed. The spring 32 intermittently deforms and applies the deformation force to the movable frame 3. The movable frame 3 drives the support rod 36. The support rod 36 drives multiple ring blocks 37 to rise and fall continuously. During the rise and fall, the ring blocks 37 rub against the inner wall of the tank 1, scraping away the microorganisms adhering to the inner wall of the tank 1 downwards. During the continuous rise and fall, due to the ring shape and downward conical structure of the ring blocks 37, the fluid in the tank 1 is divided into multiple vortices and the direction of the vortices is guided downwards, improving the mixing efficiency.

[0034] The upper end of the tank body 1 is provided with a feeding end 11, which is connected to the tank body 1 by fasteners, including but not limited to bolts. The bottom end of the tank body 1 is fixedly connected with a discharge end 12. Microbial inoculum is fed into the tank body 1 through the feeding end 11, and the mixed microbial inoculum is discharged through the discharge end 12. The discharge efficiency of the microbial inoculum is improved by the guidance of the ring block 37. A valve is installed on the discharge end 12.

[0035] Working Principle: Microbial inoculum is fed into tank 1 through feeding end 11. Drive motor 2 drives drive shaft 21 to rotate via output shaft. Drive shaft 21, in turn, drives corresponding first connecting rod 34 and auxiliary rod 4 to rotate. The first connecting rod 34, in turn, drives auger shaft 38 in a circular motion trajectory. Compared to horizontal and vertical straight stirring components, the spiral arrangement of auger shaft 38 increases the shear and extrusion forces on the microbial inoculum during mixing. Furthermore, the spiral arrangement creates multiple vortices. The generation of these vortices leads to the opposing forces between them, maintaining a uniform concentration difference among the microbial inoculum and improving the mixing efficiency of the device. The microbial strains are thoroughly mixed. The first connecting rod 34 and the secondary rod 4 are driven by the rotating drive shaft 21. The first connecting rod 34 and the secondary rod 4 drive the support rod 41, which in turn drives the fixed block 42. The fixed block 42 drives the stirring group 43 and the flow divider 44 to rotate and come into contact with the microbial strains. The toothed structure of the flow divider 44 generates a multi-stage shear effect, dividing the liquid flow into fine micro-clusters. The sharp edges formed by adjacent teeth create a local high-pressure gradient during rotation, significantly enhancing the turbulent kinetic energy dissipation rate and achieving efficient mixing. The symmetrical side plates of the stirring group 43 form a Venturi-type flow channel. Combined with the irregular gaps between the teeth, it can induce counter-rotating Taylor-Green vortex pairs. This design shortens the mixing time and generates a high shear rate. The system effectively depolymerizes microbial strains. The perforated teeth of the diverter block 44 create periodic pressure pulsations during rotation, and the radius of curvature at the tooth tips generates a high Laplace pressure differential. The forced vibration acceleration at the gas-liquid interface reaches a high level, disrupting the elasticity of the foam film. A jet is generated between the teeth, and the impact force of the jet eliminates the foam. The microchannels formed between the teeth create Dean's vortexes, increasing bubble migration speed and surface renewal frequency, thus reducing the foam phase content. This prevents the formation of a large amount of foam covering the liquid surface during stirring, which would hinder oxygen dissolution and limit the growth of aerobic bacteria. The foam layer obstructs liquid flow. The first connecting rod 34, during rotation, drives the second abutting block 35 to abut against the first abutting block 33. Intermittent transmission occurs during this abutment. The movable frame 3 is pushed up and down intermittently. During the intermittent lifting and lowering of the movable frame 3, the spring 32 is compressed intermittently. The spring 32 deforms intermittently and applies the force of the deformation to the movable frame 3. The movable frame 3 drives the support rod 36, which drives multiple ring blocks 37 to continuously lift and lower. During the lifting and lowering, the ring blocks 37 rub against the inner wall of the tank 1, scraping the microbial inoculum adhering to the inner wall of the tank 1 downwards. During the continuous lifting and lowering, due to the ring shape and downward conical structure of the ring blocks 37, the fluid in the tank 1 is divided into multiple vortices and the direction of the vortices is guided downwards, which improves the mixing efficiency. The mixed microbial inoculum is discharged through the discharge end 12, and the discharge efficiency of the microbial inoculum is improved by the guidance of the ring blocks 37. A valve is installed on the discharge end 12.

[0036] It is understood that this utility model has been described through some embodiments, and those skilled in the art will recognize that various changes or equivalent substitutions can be made to these features and embodiments without departing from the spirit and scope of this utility model. Furthermore, under the teachings of this utility model, these features and embodiments can be modified to adapt to specific situations and materials without departing from the spirit and scope of this utility model. Therefore, this utility model is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the protection scope of this utility model.

Claims

1. An experimental apparatus for the proportioning of microbial strains, characterized in that, include: Tank body (1), with an installation position inside the tank body (1); The mixing component is set inside the tank (1) and is used to mix microbial strains in proportion. The mixing component includes a drive motor (2), a movable frame (3), an auger shaft (38) and a fixed block (42). The drive motor (2) is fixedly connected to the tank (1). The movable frame (3) is set inside the tank (1) and is connected to the drive motor (2) in a transmission manner. An array of auger shafts (38) is set inside the tank (1), and each auger shaft (38) is connected to the drive motor (2) in a transmission manner. The fixed blocks (42) are symmetrically set inside the tank (1), and each fixed block (42) is connected to the drive motor (2) in a transmission manner.

2. The experimental apparatus for microbial strain proportioning according to claim 1, characterized in that, The output shaft of the drive motor (2) is fixedly connected to the drive shaft (21), the drive shaft (21) is rotatably connected to the tank (1), the drive shaft (21) is arranged in an array and symmetrically with first connecting rods (34), and the drive shaft (21) is also arranged in an array with secondary rods (4), each of the first connecting rods (34) and secondary rods (4) is fixedly connected to the drive shaft (21).

3. The experimental apparatus for microbial strain proportioning according to claim 2, characterized in that, Each of the opposing first connecting rods (34) is fixedly connected to a corresponding auger shaft (38), and each auger shaft (38) is oriented in opposite directions.

4. The experimental apparatus for microbial strain proportioning according to claim 2, characterized in that, Each of the corresponding first connecting rods (34) and the secondary rods (4) is fixedly connected to a support rod (41). Each support rod (41) is provided with an array of fixing blocks (42). Each fixing block (42) is symmetrically provided with a stirring group (43). Each stirring group (43) is fixedly connected to an end block (45) at its end.

5. The experimental apparatus for microbial strain proportioning according to claim 4, characterized in that, Each of the stirring units (43) consists of two symmetrical side plates, with multiple diverter blocks (44) fixedly connected between the side plates, and each diverter block (44) forming a hollow tooth-like structure.

6. The experimental apparatus for microbial strain proportioning according to claim 2, characterized in that, Each of the first connecting rods (34) has a second abutting block (35) fixedly connected to its upper end, each of the second abutting blocks (35) abutting against a first abutting block (33), and each of the first abutting blocks (33) is fixedly connected to the movable frame (3).

7. The experimental apparatus for microbial strain proportioning according to claim 1, characterized in that, The inner wall of the tank (1) is fixedly connected to an installation frame (31). Multiple springs (32) are provided between the movable frame (3) and the installation frame (31). The end of each spring (32) is fixedly connected to the movable frame (3) and the installation frame (31) respectively. The movable frame (3) and the installation frame (31) are slidably connected.

8. The experimental apparatus for microbial strain proportioning according to claim 1, characterized in that, The movable frame (3) is symmetrically provided with support rods (36), each support rod (36) is fixedly connected to the movable frame (3), and each support rod (36) is provided with an array of ring blocks (37), each ring block (37) is fixedly connected to the corresponding support rod (36).

9. The experimental apparatus for microbial strain proportioning according to claim 8, characterized in that, Each of the ring blocks (37) is ring-shaped as a whole, and the ring blocks (37) are cone-shaped downwards. Each ring block (37) is in close contact with the inner wall of the tank (1).

10. The experimental apparatus for microbial strain proportioning according to claim 1, characterized in that, The upper end of the tank (1) is provided with a material conveying end (11), which is connected to the tank (1) by fasteners, and the bottom end of the tank (1) is fixedly connected with a discharge end (12).

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