A cytokine flow cytometry detection kit

Abstract

This utility model belongs to the field of medical device technology, and particularly relates to a cytokine flow cytometry detection kit, including a reagent bottle and a storage box. The reagent bottle is snapped into a limiting tube. A partition plate is snapped into the inside of the storage box, and a bottle holder slot is opened on the partition plate. The bottom of the reagent bottle is inserted into the bottle holder slot. A limiting cardboard is movably set on the inner wall of the storage box. The bottom of the partition plate abuts against the bottom of the inner wall of the storage box, and the top abuts against the bottom of the limiting cardboard. In this utility model, the limiting tube can limit the pipette tip, preventing the pipette tip from falling into the reagent bottle during the process of using the pipette to draw liquid, which would prevent the operator from being unable to quickly remove the tip. The limiting cardboard can limit the top of the partition plate, preventing the partition plate from being pulled out with the bottle when the operator pulls the reagent bottle out of the bottle holder slot.

Description

Technical Field

[0001] This invention belongs to the field of medical device technology, and in particular relates to a cytokine flow cytometry detection kit. Background Technology

[0002] Commercially available cytokine flow cytometry kits are generally divided into seven-item and twelve-item cytokine flow cytometry kits. They are mainly used to simultaneously and quantitatively detect cytokines in human serum, plasma, and cell culture supernatants, assisting laboratory personnel and medical staff in assessing the state of human immune function. During the experiment, a pipette is needed to aspirate the reaction solution from the reagent bottle. When aspirating, the pipette tip is prone to falling into the reagent bottle. Due to the limitation of the bottle opening, the staff cannot quickly remove the pipette tip that has fallen into the bottle, which affects the normal progress of the experiment.

[0003] In existing technologies, the reagent kits used to store test reagent bottles are relatively simple in structure. Typically, a divider with sockets is placed inside the kit to hold the reagent bottle in place. However, in practice, the divider lacks proper fixation, causing it to move upwards with the bottle when it is removed. This hinders the smooth removal of the bottle and requires the operator to hold the divider with one hand while using the other to retrieve the bottle, a cumbersome process. Furthermore, when the last bottle is removed, the divider is often pulled out with it, requiring the operator to reinstall it back into the kit, which is also inconvenient.

[0004] Secondly, in the existing technology, due to the different types of test reagents, the capacity and height of each reagent bottle are different, and the caps of some reagent bottles are set at the bottle opening by a snap-fit ​​method. However, the reagent kit only uses a divider to limit the body of the reagent bottle, and lacks a limit for the cap. When the cardboard box storing the reagent kit is accidentally tilted or tipped over, the shorter reagent bottle will detach from the socket and collide with other reagent bottles, causing the body of the reagent bottle to be damaged due to the impact. At the same time, when the collision occurs, the cap snapped to the top of the reagent bottle will loosen due to the impact and open by itself, causing the reaction liquid in the reagent bottle to leak. Utility Model Content

[0005] (a) Technical problems to be solved

[0006] This invention provides a cytokine flow cytometry detection kit to solve the following problems:

[0007] 1. When using a pipette to aspirate the reaction solution from a reagent bottle, the pipette tip is prone to falling into the reagent bottle. Due to the restriction of the bottle opening, the staff cannot quickly remove the pipette tip that has fallen into the bottle.

[0008] 2. Existing reagent kits lack a fixing device for the separator plate used to limit the reagent bottle. When the reagent bottle is removed, the separator plate is easy to move upward with it or be taken out with it.

[0009] 3. Existing reagent kits lack cap restraint. When the kit is tilted, the bottle body is prone to detaching from the separator and being bumped, causing damage to the bottle body. The cap may also open due to the impact, resulting in leakage of the reaction solution.

[0010] (II) Technical Content

[0011] To achieve the above objectives, this utility model provides the following technical solution:

[0012] A flow cytometry assay kit for cytokines includes a reagent bottle and a limiting tube. The tops of both the reagent bottle and the limiting tube are open. The top of the reagent bottle has a concave annular groove. The limiting tube is placed in the reagent bottle. A retaining ring is fixed to the outer wall of the top of the limiting tube. The top of the limiting tube is engaged with the concave annular groove through the retaining ring.

[0013] The wall of the limiting tube has several perforated holes, and the inner cavity of the limiting tube is connected to the inner cavity of the reagent bottle through the perforated holes.

[0014] The top of the retaining ring has a first recess, and a lifting strip is placed on the first recess. One side of the lifting strip is fixed to the inner wall of the first recess.

[0015] The reagent bottle is equipped with a cap on top;

[0016] It also includes a storage box for storing reagent bottles. The storage box has a partition plate inside, and the partition plate has a bottle holder slot. The reagent bottles are inserted into the bottle holder slot. The inner wall of the storage box is movably provided with a limiting cardboard. The bottom of the partition plate abuts against the bottom of the inner wall of the storage box, and the top abuts against the bottom of the limiting cardboard.

[0017] Furthermore, the bottom of the limiting tube contacts the bottom of the inner cavity of the reagent bottle.

[0018] Furthermore, both the retaining ring and the limiting tube are transparent.

[0019] Furthermore, when the retaining ring is inserted into the concave groove, the top end face of the retaining ring is not higher than the top end face of the reagent bottle. A sealing film is affixed to the top of the reagent bottle, and the inner cavity of the reagent bottle and the limiting tube are isolated from the outside through the sealing film.

[0020] Furthermore, the edges of the divider are bent downwards to form a rectangular box with the opening facing downwards. The divider opening faces downwards and is secured to the bottom of the inner cavity of the storage box.

[0021] The top of the partition plate has multiple bottle holder slots, and reagent bottles are inserted into each of the multiple bottle holder slots.

[0022] The bottoms of multiple reagent bottles are all in contact with the bottom of the inner cavity of the storage box.

[0023] Furthermore, a second recess is provided on the two opposite inner side walls of the storage box, and a limiting cardboard is rotatably connected to one vertical side edge of the inner wall of the second recess.

[0024] The limiting cardboard is adapted to the second recess;

[0025] The top of the limiting cardboard has a notch.

[0026] Furthermore, a connecting plate is fitted into the top of the inner cavity of the storage box. The connecting plate is located above the reagent bottle. The connecting plate is hollow inside and open at the top. Multiple hollow pressure columns are integrally set at the bottom of the connecting plate. Each hollow pressure column corresponds to a reagent bottle. The top of the bottle cap on the reagent bottle abuts against the bottom of the corresponding hollow pressure column.

[0027] A cover plate is rotatably connected to the top side of the storage box. A third recess is opened on one side of the storage box. A first magnet is embedded in the inner wall of the third recess. A lifting strap is fixed to one end of the cover plate near the third recess. A second magnet is embedded in the lifting strap. The magnetic poles of the first magnet and the second magnet are opposite. The lifting strap is placed in the third recess, and the first magnet and the second magnet are magnetically attracted together.

[0028] The top of the connecting plate is flush with the top of the storage box, and the top of the connecting plate abuts against the bottom of the cover plate.

[0029] Furthermore, the bottle cap is fastened to the top of the corresponding reagent bottle, and a traction strip is fixed to the outer wall of the top of the reagent bottle, with the free end of the traction strip fixed to the outer wall of the corresponding bottle cap.

[0030] Furthermore, the inner walls of multiple bottle caps are provided with internal threads, and the top of the outer walls of multiple reagent bottles are provided with external threads. The bottle caps are connected to the corresponding reagent bottles by the threads.

[0031] (III) Beneficial Effects

[0032] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0033] 1. In this utility model, when the suction head falls into the limiting tube, the user can pull the lifting strip upward to remove the limiting tube from the reagent bottle, thereby removing the fallen suction head from the reagent bottle. After removing the suction head from the limiting tube, the user can then use the lifting strip to re-engage the retaining ring with the concave groove. After engagement, the suction of the reaction liquid can continue.

[0034] Second, in this utility model, when installing the partition plate, first rotate the limiting cardboard so that it is locked in the corresponding second inner recess. The limiting cardboard can be stored by setting the second inner recess, so that the partition plate can be placed at the bottom of the inner cavity of the storage box. After placement, the limiting cardboard is pushed outward by the notch, thereby pulling the limiting cardboard out of the second inner recess. At this time, the top of the partition plate abuts against the bottom of the limiting cardboard. The limiting cardboard can limit the top of the partition plate, preventing the partition plate from being pulled out with the bottle when the staff pulls the reagent bottle out of the bottle holder.

[0035] Third, in this utility model, multiple hollow pressure columns set at the bottom of the connecting plate can simultaneously limit the top of the bottle caps at different heights, preventing the storage box from tilting or tipping over due to accidents, and preventing reagent bottles with lower body height from falling out of the corresponding bottle holder and colliding with other reagent bottles, which could lead to damage to the reagent bottle or its cap opening due to impact, causing leakage of the reaction liquid.

[0036] Fourth, in this utility model, both the retaining ring and the limiting tube are transparent structures, so that staff can clearly observe the inside of the reagent bottle from the top opening, ensuring that staff can promptly detect whether impurities have fallen into the reagent bottle during storage and use.

[0037] Fifth, in this utility model, the connecting plate is hollow inside and open at the top, which reduces production costs and also serves as a storage function, so that staff can store and carry the items needed for the experiment (including but not limited to pipettes) in the internal space of the connecting plate. Attached Figure Description

[0038] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0039] Figure 2 This is a schematic diagram of the overall structure of the present invention when the cover plate is in the open state;

[0040] Figure 3 This is a partial cross-sectional view of the cardboard box in this utility model;

[0041] Figure 4 This is a partial cross-sectional view of the cardboard box in this utility model from another perspective;

[0042] Figure 5 This is a schematic diagram of the structure of the reagent bottle and the retaining ring after they are engaged in this utility model;

[0043] Figure 6 This is an explosion diagram of the limiting tube and reagent bottle in this utility model;

[0044] Figure 7 This is a schematic diagram of the structure of the reagent bottle and sealing film in this utility model;

[0045] Figure 8 This is a schematic diagram of the partition plate in this utility model;

[0046] Figure 9 This is a schematic diagram of the structure of the present invention when the partition plate is placed at the bottom of the inner cavity of the storage box;

[0047] Figure 10 This is a schematic diagram of the structure of the present invention when the limiting cardboard is pulled out from the second concave opening;

[0048] Figure 11 This is a schematic diagram of the connecting plate and hollow pressure column in this utility model;

[0049] Figure 12 This is a schematic diagram of the assembly of the reagent bottle and bottle cap provided in Embodiment 1 of this utility model;

[0050] Figure 13 This is a schematic diagram of the assembly of the reagent bottle and bottle cap provided in Embodiment 2 of this utility model;

[0051] Figure 14 This is an exploded schematic diagram of the reagent bottle and bottle cap provided in Embodiment 2 of this utility model.

[0052] In the diagram: 11. Reagent bottle; 1101. Concave annular groove; 111. Traction strip; 12. Bottle cap; 21. Limiting tube; 2101. First concave opening; 211. Clamping ring; 212. Lifting strip; 31. Sealing film; 41. Storage box; 4101. Second concave opening; 4102. Third concave opening; 411. Limiting cardboard; 412. Cover plate; 413. Lifting strap; 42. First magnet; 43. Second magnet; 51. Divider plate; 5101. Bottle holder clamp; 61. Connecting plate; 611. Hollow pressure column. Detailed Implementation

[0053] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0054] Example 1

[0055] like Figures 1-14 As shown, a cytokine flow cytometry assay kit includes a reagent bottle 11 and a limiting tube 21, as follows: Figure 5 and Figure 6 As shown, both the reagent bottle 11 and the limiting tube 21 have open tops. The top of the reagent bottle 11 has a concave annular groove 1101. The limiting tube 21 is placed in the reagent bottle 11. A retaining ring 211 is fixed to the outer wall of the top of the limiting tube 21. The top of the limiting tube 21 is engaged with the concave annular groove 1101 through the retaining ring 211. During the experiment, a pipette is needed to aspirate the reaction liquid in the reagent bottle 11. By setting the limiting tube 21, the pipette tip can be limited, preventing the pipette tip from falling into the reagent bottle 11 during the aspiration of liquid, which would prevent the staff from quickly removing the tip.

[0056] The concave annular groove 1101 can limit the retaining ring 211, thereby limiting the top of the limiting tube 21 and preventing the limiting tube 21 from shifting randomly in the inner cavity of the reagent bottle 11, which would affect the staff's ability to put the aspiration head into the limiting tube 21.

[0057] The wall of the limiting tube 21 is provided with several hollow holes. The inner cavity of the limiting tube 21 is connected to the inner cavity of the reagent bottle 11 through the hollow holes. Under the action of the hollow holes, the limiting tube 21 will only limit the pipette tip, but will not obstruct the flow of the reaction liquid, so that the pipette can draw the reaction liquid from the reagent bottle 11.

[0058] The top of the retaining ring 211 has a first concave opening 2101, and a lifting strip 212 is placed on the first concave opening 2101. One side of the lifting strip 212 is fixed to the inner wall of the first concave opening 2101. The lifting strip 212 facilitates the operator to remove the limiting tube 21 with the retaining ring 211 from the reagent bottle 11. When the suction head falls into the limiting tube 21, the user can pull the lifting strip 212 upward to remove the limiting tube 21 from the reagent bottle 11, thereby removing the fallen suction head from the reagent bottle 11. After removing the suction head from the limiting tube 21, the retaining ring 211 is re-engaged with the concave ring groove 1101 by the lifting strip 212. After engagement, the suction of the reaction solution can continue.

[0059] The reagent bottle 11 is provided with a cap 12 at the top. The cap 12 can seal the top of the reagent bottle 11 to prevent small impurities from the outside from falling into the reagent bottle 11 during storage.

[0060] Furthermore: the bottom of the limiting tube 21 contacts the bottom of the inner cavity of the reagent bottle 11, so that the pipette can draw the reaction liquid from the bottom of the reagent bottle 11.

[0061] Furthermore, both the retaining ring 211 and the limiting tube 21 are transparent, so that staff can clearly observe the inside of the reagent bottle 11 from the top opening, ensuring that staff can promptly detect whether impurities have fallen into the inside of the reagent bottle 11 during storage and use.

[0062] Furthermore, such as Figure 7 As shown, when the retaining ring 211 is inserted into the concave annular groove 1101, the top end face of the retaining ring 211 is not higher than the top end face of the reagent bottle 11. A sealing film 31 is attached to the top of the reagent bottle 11, and the inner cavity of the reagent bottle 11 and the limiting tube 21 are isolated from the outside through the sealing film 31.

[0063] During production, after the limiting tube 21 is inserted into the reagent bottle 11 via the retaining ring 211, a sealing film 31 is affixed to the top of the reagent bottle 11. The sealing film 31 isolates the inner cavity of the reagent bottle 11 and the limiting tube 21 from the outside world, preventing dust and other small impurities in the air from falling into the reagent bottle 11 during production and transportation. When using the reagent bottle, the user only needs to tear off the sealing film 31 from the top of the reagent bottle 11.

[0064] When the retaining ring 211 is inserted into the concave annular groove 1101, the top end face of the retaining ring 211 is not higher than the top end face of the reagent bottle 11, so that the sealing film 31 can be flatly attached to the top end face of the reagent bottle 11 during production, reducing the difficulty of attaching the sealing film 31.

[0065] Combination Figure 3 , Figure 4 , Figure 9 and Figure 10 The cytokine flow cytometry assay kit also includes a storage box 41 for storing reagent bottles 11. A divider 51 is inserted inside the storage box 41. A bottle holder slot 51 is provided on the divider 51. The reagent bottle 11 is inserted into the bottle holder slot 5101. Furthermore, the edge of the divider 51 is bent downward to form a rectangular box with the opening facing downward. The divider 51 is placed at the bottom of the inner cavity of the storage box 41 with the opening facing downward.

[0066] The top of the partition plate 51 is provided with multiple bottle holder slots 5101, and each of the multiple bottle holder slots 5101 is inserted with a reagent bottle 11. The multiple bottle holder slots 5101 are provided to facilitate the placement of reagent bottles 11 containing different reaction solutions by the staff.

[0067] The partition plate 51 is positioned with one open end facing downwards, which increases the height of the bottle holder 5101. After the reagent bottle 11 is inserted into the corresponding bottle holder 5101, the bottle holder 5101 can form a circumferential restraint on the lower part of the reagent bottle 11, preventing the reagent bottle 11 from tilting or tipping over in the storage box 41, which could lead to leakage of the test liquid inside the bottle.

[0068] The inner wall of the storage box 41 is movably provided with a limiting cardboard 411. The bottom of the partition plate 51 abuts against the bottom of the inner wall of the storage box 41 and the top abuts against the bottom of the limiting cardboard 411. The bottom and top of the partition plate 51 are limited by the bottom of the inner wall of the storage box 41 and the limiting cardboard 411 respectively, so as to prevent the partition plate 51 from moving freely in the storage box 41.

[0069] Furthermore, the storage box 41 has two opposite inner walls with a second recess 4101, and a limiting cardboard 411 is rotatably connected to one vertical side edge of the inner wall of the second recess 4101.

[0070] The limiting cardboard 411 is adapted to the second recess 4101;

[0071] The top of the limiting cardboard 411 is notched. When installing the divider 51, first rotate the limiting cardboard 411 so that it is locked in the corresponding second recess 4101. The second recess 4101 can be used to store the limiting cardboard 411 so that the divider 51 can be placed at the bottom of the inner cavity of the storage box 41. After placement, the limiting cardboard 411 is pushed outward by the notch, so that the limiting cardboard 411 is pulled out from the second recess 4101. At this time, the top of the divider 51 abuts against the bottom of the limiting cardboard 411. The limiting cardboard 411 can limit the top of the divider 51, so as to prevent the divider 51 from being pulled out with the bottle when the staff pulls the reagent bottle 11 out of the bottle holder slot 5101.

[0072] Furthermore, such as Figure 12 As shown, the bottle cap 12 is fastened to the top of the corresponding reagent bottle 11. A traction strip 111 is fixed to the outer wall of the top of the reagent bottle 11. The free end of the traction strip 111 is fixed to the outer wall of the corresponding bottle cap 12. The bottle cap 12 is fastened to the reagent bottle 11 by a snap-fit ​​method. Whether opening the bottle cap 12 or closing it again, the disassembly and closure can be completed quickly. The traction strip 111 can limit the bottle cap 12, preventing it from falling off accidentally after being opened from the top of the reagent bottle 11 and thus being lost.

[0073] After the experiment, reagent bottle 11 was reinserted into the corresponding bottle holder 5101, combined with... Figures 1-3 , Figure 11 A connecting plate 61 is fitted into the top of the inner cavity of the storage box 41. The connecting plate 61 is located above the reagent bottle 11. The connecting plate 61 is hollow inside and open at the top, thereby reducing the material consumed when making the connecting plate 61 and reducing production costs. Since the types of test reagents are different, the capacity and height of each reagent bottle 11 are also different. Multiple hollow pressure columns 611 are integrally set at the bottom of the connecting plate 61. The multiple hollow pressure columns 611 correspond one-to-one with the multiple reagent bottles 11. The top of the bottle cap 12 set on the reagent bottle 11 abuts against the bottom of the corresponding hollow pressure column 611.

[0074] The bottom of the reagent bottle 11 can be supported by the bottom of the inner cavity of the storage box 41;

[0075] The bottle holder 5101 can form a circumferential restraint on the lower part of the body of the reagent bottle 11;

[0076] Multiple hollow pressure columns 611 set at the bottom of the connecting plate 61 can simultaneously limit the top of the bottle cap 12 located at different heights, preventing the reagent bottle 11 with a lower body height from coming out of the corresponding bottle holder 5101 and colliding with other reagent bottles 11 when the storage box 41 is accidentally tilted or tipped over, causing damage to the reagent bottle 11, and preventing the bottle cap 12 at the top of the reagent bottle 11 from loosening and opening on its own due to the impact, resulting in liquid leakage from the reagent bottle 11;

[0077] The connecting plate 61 can move multiple hollow pressure columns 611 together. The connecting plate 61 is hollow inside and open at the top, which reduces production costs and can also serve as a storage function, so that staff can store and carry the items needed for the experiment (including but not limited to pipettes) in the internal space of the connecting plate 61.

[0078] like Figures 1-3 As shown, a cover plate 412 is rotatably connected to one side of the top of the storage box 41. The cover plate 412 can seal the top of the storage box 41 to prevent external debris from falling into the storage box 41. A third recess 4102 is provided on one side of the storage box 41. A first magnet 42 is embedded in the inner wall of the third recess 4102. A lifting strap 413 is fixed to one end of the cover plate 412 near the third recess 4102. A second magnet 43 is embedded in the lifting strap 413. The magnetic poles of the first magnet 42 and the second magnet 43 are opposite. The lifting strap 413 is placed in the third recess 4102, and the first magnet 42 and the second magnet 43 are magnetically attracted together, thereby limiting the position of the cover plate 412 and fixing the cover plate 412 to the top of the storage box 41.

[0079] The top of the connecting plate 61 is flush with the top of the storage box 41 so that the cover plate 412 can be placed flat on the top of the storage box 41. After the cover plate 412 is fixed to the top of the storage box 41 under the action of the first magnet 42 and the second magnet 43, the top of the connecting plate 61 abuts against the bottom of the cover plate 412. The cover plate 412 limits the connecting plate 61 to prevent the connecting plate 61 from falling out of the storage box 41 when the storage box 41 is tilted or tipped over, so that the hollow pressure column 611 cannot properly limit the corresponding bottle cap 12.

[0080] Example 2

[0081] like Figures 1-14 As shown, this embodiment has been improved on the basis of embodiment one as follows: further, the inner walls of the plurality of bottle caps 12 are provided with internal threads, and the top of the outer walls of the plurality of reagent bottles 11 are provided with external threads, and the bottle caps 12 are connected to the corresponding reagent bottles 11 by threads.

[0082] The cap 12 is threaded to the top of the corresponding reagent bottle 11. Although the speed of disassembling and installing the cap 12 is slower than that of directly snapping the cap 12 to the top of the corresponding reagent bottle 11, it can effectively improve the firmness of the cap 12 on the reagent bottle 11 and prevent the cap 12 from opening naturally due to bumps, thus preventing liquid leakage.

[0083] Staff can choose the appropriate way to connect the bottle cap 12 to the corresponding reagent bottle 11 according to the actual usage scenario.

[0084] In summary, the workflow of this utility model is as follows:

[0085] During assembly: First, rotate the limiting cardboard 411 so that it is locked in the corresponding second recess 4101. Then, place the partition plate 51 at the bottom of the inner cavity of the storage box 41. After placement, push the limiting cardboard 411 outward through the notch to pull it out of the second recess 4101. At this time, the top of the partition plate 51 abuts against the bottom of the limiting cardboard 411, thereby limiting the top of the partition plate 51. Then, insert the reagent bottle 11 into the corresponding bottle holder slot 5101. After placement, lock the connecting plate 61 into the storage box 41. At this time, the hollow pressure column 611 located on the connecting plate 61 will limit the bottle cap 12 set on the top of the reagent bottle 11. Then, cover the top of the storage box 41 with the cap plate 412 and lock the lifting strap 413 into the third recess 4102 so that the first magnet 42 and the second magnet 43 are magnetically attracted together.

[0086] In use: During the experiment, the pipette is used to aspirate the reaction solution in reagent bottle 11. The reaction solution in reagent bottle 11 flows into the limiting tube 21 through the perforated hole for the pipette to aspirate. When the aspirator tip falls into the limiting tube 21, the user can pull up the lifting strip 212 to remove the limiting tube 21 from the reagent bottle 11, thereby removing the fallen aspirator tip from the reagent bottle 11. After removing the aspirator tip from the limiting tube 21, the retaining ring 211 is re-engaged with the concave annular groove 1101 by the lifting strip 212. After engagement, the aspiration of the reaction solution can continue.

[0087] The different embodiments described above can be combined, substituted, or used in combination with each other.

[0088] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0089] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cytokine flow cytometry detection kit, characterized in that: The reagent bottle (11) and the limiting tube (21) are both open at the top. The top of the reagent bottle (11) is provided with an inner concave annular groove (1101). The limiting tube (21) is placed in the reagent bottle (11). A retaining ring (211) is fixed to the outer wall of the top of the limiting tube (21). The top of the limiting tube (21) is engaged with the inner concave annular groove (1101) through the retaining ring (211). The wall of the limiting tube (21) is provided with several hollow holes, and the inner cavity of the limiting tube (21) is connected to the inner cavity of the reagent bottle (11) through the hollow holes; The top of the retaining ring (211) is provided with a first recess (2101), and a lifting strip (212) is placed on the first recess (2101). One side of the lifting strip (212) is fixed to the inner wall of the first recess (2101). The reagent bottle (11) is provided with a cap (12) on the top. It also includes a storage box (41) for storing reagent bottles (11). A partition plate (51) is inserted inside the storage box (41). A bottle holder slot (5101) is opened on the partition plate (51). The reagent bottle (11) is inserted into the bottle holder slot (5101). A limiting cardboard (411) is movably provided on the inner wall of the storage box (41). The bottom of the partition plate (51) abuts against the bottom of the inner wall of the storage box (41), and the top abuts against the bottom of the limiting cardboard (411).

2. The cytokine flow cytometry detection kit according to claim 1, characterized in that: The bottom of the limiting tube (21) is in contact with the bottom of the inner cavity of the reagent bottle (11).

3. The cytokine flow cytometry detection kit according to claim 2, characterized in that: Both the retaining ring (211) and the limiting tube (21) are transparent structures.

4. The cytokine flow cytometry detection kit according to claim 2, characterized in that: When the retaining ring (211) is inserted into the concave annular groove (1101), the top end face of the retaining ring (211) is not higher than the top end face of the reagent bottle (11). The top of the reagent bottle (11) is covered with a sealing film (31), and the inner cavity of the reagent bottle (11) and the limiting tube (21) are isolated from the outside through the sealing film (31).

5. The cytokine flow cytometry detection kit according to claim 1, characterized in that: The edge of the partition (51) is bent downward to form a rectangular box with the opening facing downward. The partition (51) is positioned with the opening facing downward and is placed at the bottom of the inner cavity of the storage box (41). The top of the partition plate (51) is provided with multiple bottle holder slots (5101), and a reagent bottle (11) is inserted into each of the multiple bottle holder slots (5101). The bottoms of multiple reagent bottles (11) are in contact with the bottom of the inner cavity of the storage box (41).

6. The cytokine flow cytometry detection kit according to claim 5, characterized in that: The storage box (41) has a second recess (4101) on its two opposite inner side walls, and a limiting cardboard (411) is rotatably connected to one vertical side edge of the inner wall of the second recess (4101). The limiting cardboard (411) is adapted to the second recess (4101); The top of the limiting cardboard (411) is provided with a notch.

7. The cytokine flow cytometry detection kit according to claim 5, characterized in that: A connecting plate (61) is placed on the top of the inner cavity of the storage box (41). The connecting plate (61) is located above the reagent bottle (11). The connecting plate (61) is hollow inside and open at the top. Multiple hollow pressure columns (611) are integrally provided at the bottom of the connecting plate (61). The multiple hollow pressure columns (611) correspond one-to-one with the multiple reagent bottles (11). The top of the bottle cap (12) on the reagent bottle (11) abuts against the bottom of the corresponding hollow pressure column (611). A cover plate (412) is rotatably connected to the top side of the storage box (41). A third recess (4102) is opened on one side of the storage box (41). A first magnet (42) is embedded in the inner wall of the third recess (4102). A lifting strap (413) is fixed to one end of the cover plate (412) near the third recess (4102). A second magnet (43) is embedded in the lifting strap (413). The magnetic poles of the first magnet (42) and the second magnet (43) are opposite. The lifting strap (413) is placed in the third recess (4102), and the first magnet (42) and the second magnet (43) are magnetically attracted together. The top of the connecting plate (61) is flush with the top of the storage box (41), and the top of the connecting plate (61) abuts against the bottom of the cover plate (412).

8. The cytokine flow cytometry detection kit according to claim 7, characterized in that: The bottle cap (12) is fastened to the top of the corresponding reagent bottle (11), and a traction strip (111) is fixed to the outer wall of the top of the reagent bottle (11). The free end of the traction strip (111) is fixed to the outer wall of the corresponding bottle cap (12).

9. The cytokine flow cytometry detection kit according to claim 7, characterized in that: The inner walls of the multiple bottle caps (12) are provided with internal threads, and the top of the outer walls of the multiple reagent bottles (11) are provided with external threads. The bottle caps (12) are connected to the corresponding reagent bottles (11) by threads.

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