A kit for an in vitro transcription reaction of mRNA

Abstract

The utility model discloses a mRNA in vitro transcription reaction kit, including the placing box, is provided with the receiving block in the placing box, is provided with test tube hole on the receiving block, and the both sides of placing box are slidably connected with the seal, and the one side of seal is connected with the limiting strip, and the spring is connected between the limiting strip and the placing box, and the limiting mechanism that is used for limiting the test tube in test tube hole is connected on the receiving block, and the seal is connected with the wrenching block, and the limiting mechanism includes the gyro wheel, and the gyro wheel is rotatably connected on the receiving block through the rotating shaft, and the one end of rotating shaft is connected with the gear, and the rack is connected on the inner wall of placing box. The utility model discloses utilize the gyro wheel to be able to good to test tube and carry out the clamping action, cooperate the rubber ring to be able to effectively reduce the structure wear and tear, and improve the limiting stability, utilize the gear structure to guarantee the unity of limiting, make each test tube be a whole, improve the stability of transportation.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, and in particular to an mRNA in vitro transcription reaction kit. Background Technology

[0002] The mRNA in vitro transcription reaction kit is an integrated biotechnology product containing reagents such as RNA polymerase, nucleotides, and buffer solutions, along with a complete operating protocol. It enables efficient in vitro synthesis of mRNA using DNA as a template. Its core function is to achieve controlled mRNA synthesis through optimized reaction systems. It is widely used in vaccine development, gene therapy, and other fields, serving as a key tool for the transformation of biomedical technologies.

[0003] Traditional kits for in vitro mRNA transcription reactions are mainly designed to prevent the use of reagent tubes, facilitating their collection or storage and ensuring the stability of the tube structure during the reaction. However, traditional kits have a simple structure, with no additional restraint mechanisms for the tubes inside, making them prone to shaking when the kit is moved. Furthermore, the kits have poor transport stability, allowing internal tubes to easily fall out and result in losses.

[0004] Based on this, an in vitro mRNA transcription reaction kit is proposed. Utility Model Content

[0005] The purpose of this invention is to provide an in vitro mRNA transcription reaction kit to solve the above-mentioned problems.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] An mRNA in vitro transcription reaction kit includes a placement box, a storage block disposed inside the placement box, a test tube well on the storage block, a sealing strip slidably connected to both sides of the placement box, a limiting strip connected to one side of the sealing strip, a spring connected between the limiting strip and the placement box, and a limiting mechanism connected to the storage block for limiting the test tubes in the test tube well.

[0008] Preferably, the seal is connected to a lever block.

[0009] Preferably, the limiting mechanism includes a roller, which is rotatably connected to the storage block via a rotating shaft. One end of the rotating shaft is connected to a gear, and a rack is connected to the inner wall of the placement box.

[0010] Preferably, a rubber ring is connected to the outside of the roller, and the rubber ring abuts against the outside of the test tube.

[0011] Preferably, the storage block has a rotating hole, and the rotating shaft is rotatably connected to the rotating hole.

[0012] Preferably, the storage block has a docking groove, the gear is stored in the docking groove, and the gears on adjacent storage blocks mesh with each other.

[0013] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0014] 1. This application adopts a roller structure, which can effectively clamp the test tubes. The rubber ring can effectively reduce structural wear and improve the stability of the limit. The gear structure ensures the uniformity of the limit, making each test tube a whole and improving the stability of transportation.

[0015] 2. This application employs a sealing structure to limit the position of the storage block structure. At the same time, it utilizes a rack and pinion structure to ensure that the entire test tube will not separate from the storage block when the sealing strip limits the position of the storage block, thus maintaining the integrity of each structure. Attached Figure Description

[0016] Figure 1 A schematic diagram of the overall structure of the reagent kit provided according to an embodiment of the present invention is shown;

[0017] Figure 2 A schematic diagram of the structure of the docking groove connection provided according to an embodiment of the present utility model is shown;

[0018] Figure 3 An exploded structural diagram of the roller connection provided according to an embodiment of the present invention is shown;

[0019] Figure 4 A schematic diagram of the rack and pinion connection provided according to an embodiment of the present invention is shown.

[0020] Legend:

[0021] 1. Placement box; 2. Storage block; 3. Test tube hole; 4. Seal; 5. Limiting strip; 6. Actuating block; 7. Gear; 8. Connecting groove; 9. Rotating shaft; 10. Roller; 11. Rubber ring; 12. Rotating hole; 13. Rack. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0023] Please see Figures 1-4This utility model provides a technical solution:

[0024] An mRNA in vitro transcription reaction kit includes a placement box 1, a storage block 2 inside the placement box 1, and multiple test tube holes 3 on the storage block 2. Seals 4 are slidably connected to both sides of the placement box 1 to prevent separation between the storage block 2 and the placement box 1. A limiting strip 5 is connected to one side of the sealing strip 4, and a spring connects the limiting strip 5 to the placement box 1. The spring ensures that the sealing strip 4 is positioned above the storage block 2 without external force. A limiting mechanism is connected to the storage block 2 to limit the test tubes inside the test tube holes 3.

[0025] Specifically, such as Figure 2 As shown, a lever block 6 is connected to the seal 4, which facilitates the operator to separate the seal 4.

[0026] Specifically, such as Figure 3 As shown, the limiting mechanism includes a roller 10, which is rotatably connected to the storage block 2 via a rotating shaft 9. One end of the rotating shaft 9 is connected to a gear 7, which rotates synchronously with the roller 10. A rack 13 is connected to the inner wall of the storage box 1. Multiple racks 13 are provided, and the number of racks 13 is related to the number of gears 7 on the outer side of the storage block 2. The outer gears 7 mesh with the corresponding racks 13. The upper part of the rack 13 is a strip-shaped structure, while the lower part is a toothed strip structure that can mesh with the gears 7.

[0027] Specifically, such as Figure 3 As shown, a rubber ring 11 is connected to the outside of the roller 10. The rubber ring 11 abuts against the outside of the test tube. By setting the rubber ring 11, structural wear can be effectively reduced and the extrusion effect can be improved.

[0028] Specifically, such as Figure 3 As shown, the storage block 2 has a rotating hole 12, and the rotating shaft 9 is rotatably connected to the rotating hole 12.

[0029] Specifically, such as Figure 3 As shown, the storage block 2 has a docking groove 8, and the gear 7 is stored in the docking groove 8. The gears 7 on adjacent storage blocks 2 mesh with each other. There are two gears 7 connected to the storage block 2 corresponding to a single test tube, but the two gears 7 do not mesh with each other. The synchronous rotation between the two gears 7 is achieved by the test tube.

[0030] In summary, the mRNA in vitro transcription reaction kit provided in this embodiment allows for the transport of test tubes for the mRNA in vitro transcription reaction. Test tubes are placed uniformly in the well 3, ensuring that all wells 3 contain test tubes. The test tubes are then limited by the rubber ring 11 and cannot reach the bottom of the well 3. The receiving block 2 is then placed in the placement box 1. At this point, the gear 7 and rack 13 are in contact. As the receiving block 2 moves, the gear 7 rotates, coordinating with the roller 10 to compress the test tubes, causing each test tube to move downwards uniformly. The sealing strip 4 then limits the receiving block 2, ensuring the stability of its structural connection. The rack 13 limits the gear 7, preventing rotation and stabilizing the placement of the test tubes.

[0031] The above description of the embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An mRNA in vitro transcription reaction kit, comprising a placement box (1), characterized in that, The placement box (1) is provided with a storage block (2), and the storage block (2) has a test tube hole (3). The two sides of the placement box (1) are slidably connected with a sealing strip (4). A limit strip (5) is connected to one side of the sealing strip (4). A spring is connected between the limit strip (5) and the placement box (1). A limiting mechanism is connected to the storage block (2) to limit the test tube in the test tube hole (3).

2. The mRNA in vitro transcription reaction kit according to claim 1, characterized in that, A lever block (6) is connected to the seal (4).

3. The mRNA in vitro transcription reaction kit according to claim 1, characterized in that, The limiting mechanism includes a roller (10), which is rotatably connected to the storage block (2) via a rotating shaft (9). One end of the rotating shaft (9) is connected to a gear (7), and a rack (13) is connected to the inner wall of the placement box (1).

4. The mRNA in vitro transcription reaction kit according to claim 3, characterized in that, A rubber ring (11) is connected to the outside of the roller (10), and the rubber ring (11) abuts against the outside of the test tube.

5. The mRNA in vitro transcription reaction kit according to claim 3, characterized in that, The storage block (2) has a rotating hole (12), and the rotating shaft (9) is rotatably connected to the rotating hole (12).

6. The mRNA in vitro transcription reaction kit according to claim 3, characterized in that, The storage block (2) is provided with a docking groove (8), and the gear (7) is stored in the docking groove (8). The gears (7) on adjacent storage blocks (2) mesh with each other.

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