A nitrous oxide gas collection device with a luer quick connector acetylene quantitative and high sealing culture structure

Abstract

The utility model belongs to the technical field of culture device, especially the nitrous oxide gas collection device with luer quick -connect acetylene quantitative and high seal culture structure, the utility model discloses an acetylene supply module, soil culture module and gas collection module, the acetylene supply module includes the acetylene -filled steel bottle, is connected with the micro pressure reducing valve on the steel bottle, and the micro pressure reducing valve is connected with the luer internal thread joint through the pipeline, and the luer internal thread joint is detachably connected with the syringe, the soil culture module includes the culture cavity, and the culture cavity is equipped with the soil, and is connected with the double -way stop valve on the culture cavity, and the double -way stop valve is connected with silica gel gas injection pipe, and the silica gel gas injection pipe is inserted into the culture cavity, and the other end of double -way stop valve is connected with the luer external thread joint for detachably connecting the syringe, the utility model provides a nitrous oxide gas collection device with luer quick -connect acetylene quantitative and high seal culture structure.

Description

Technical Field

[0001] This utility model belongs to the field of culture device technology, and specifically relates to a nitrous oxide gas collection device with Luer fast-connection acetylene quantitative and highly sealed culture structure. Background Technology

[0002] Nitrous oxide (N2O) is a potent greenhouse gas, with a greenhouse effect approximately 298 times that of carbon dioxide, and is also a significant contributor to stratospheric ozone depletion. Soil is a major source of N2O emissions, with its production primarily controlled by nitrification and denitrification driven by soil microorganisms. In ecosystem nitrogen cycle research, accurately distinguishing the biological pathways of N2O is crucial for elucidating its emission mechanisms and its response to climate change. The acetylene inhibition technique (AIT), a classic method for differentiating the contributions of nitrification and denitrification, is widely used in quantitative studies of N2O emission sources in agriculture, ecology, and environmental science.

[0003] However, most commonly used acetylene inhibition experimental devices are benchtop laboratory designs, which have several limitations in structure and function. First, the acetylene gas injection method is usually rather crude, making it difficult to achieve precise control at the microliter (μL) level, affecting the accuracy of nitrification and denitrification process analysis. Second, the simple sealing structure of the device makes it prone to leakage during gas injection or sampling, resulting in large fluctuations and poor repeatability of flux measurement data. Furthermore, the sampling system interface has poor compatibility, making it impossible to achieve multiple undisturbed samplings and efficient transfer. More importantly, most devices lack real-time monitoring capabilities for environmental parameters such as temperature and humidity, failing to support the linkage analysis of dynamic environmental control and microbial activity processes, thus limiting the application of acetylene inhibition methods in in-situ, long-term, or gradient-response experiments. Utility Model Content

[0004] In order to solve the above-mentioned problems in the existing technology, the purpose of this utility model is to provide a nitrous oxide gas collection device with Luer fast-connection acetylene quantitative and highly sealed culture structure.

[0005] The technical solution adopted in this utility model is as follows:

[0006] A nitrous oxide gas collection device with a Luer quick-connect acetylene quantitative and highly sealed culture structure includes an acetylene supply module, a soil culture module, and a gas collection module. The acetylene supply module includes a steel cylinder containing acetylene, a miniature pressure reducing valve connected to the cylinder, and a Luer internal thread connector connected to the miniature pressure reducing valve via a pipe. The Luer internal thread connector is detachably connected to a syringe. The soil culture module includes a culture chamber containing soil, a two-way stopcock valve connected to the culture chamber, a silicone gas injection tube connected to the two-way stopcock valve extending into the culture chamber, and a Luer external thread connector for detachably connecting a syringe at the other end of the two-way stopcock valve.

[0007] This invention addresses the problems of imprecise acetylene injection control, unstable sealing performance, complex sampling operations, and lack of monitoring functions in existing technologies. It proposes an integrated experimental device with a compact structure, reliable sealing, precise injection, convenient sampling, and environmental monitoring capabilities, thereby improving the applicability and accuracy of the acetylene inhibition method in the study of soil N2O emission mechanisms.

[0008] This invention relates to an acetylene supply module featuring a miniature pressure reducing valve and a Luer internal thread connector, which connects to a syringe. An external Luer thread connector on the culture chamber also connects to the syringe. The miniature pressure reducing valve lowers the acetylene pressure in the cylinder to a controllable range of 0.01–0.1 MPa, while the Luer interface allows for rapid syringe connection. Users can precisely extract acetylene volumes from μL to mL using the syringe and sequentially open and close the valve to achieve gas sealing and subsequent injection operations, ensuring an operational error of ≤±2% and improving injection efficiency by approximately 300%. This invention solves the problems of difficult acetylene metering and large micro-injection errors in traditional devices, significantly improving experimental repeatability and reliability.

[0009] As a preferred embodiment of this utility model, the gas collection module includes a Luer connector, a gas collection port on the culture chamber, the Luer connector connected to the gas collection port, and a stopcock valve connected to the other end of the Luer connector via a pipe. The stopcock valve is detachably connected to a sealed syringe. The gas collection module also includes a gas collection tube and a gas storage bag connected to the gas collection tube. The other end of the gas collection tube is detachably connected to the sealed syringe. During sampling, the syringe can be connected to the gas collection port multiple times without contamination. After extracting the top gas sample, it is quantitatively transferred to the gas storage bag through the gas collection tube for subsequent gas chromatography analysis. The matching high-precision syringe and stopcock valve enable contamination-free, multiple sampling operations, and the sealed gas transfer is completed by connecting the gas storage bag through a PTFE hose, greatly improving the integrity of the N2O sample and the accuracy of subsequent analysis.

[0010] As a preferred embodiment of this utility model, the culture chamber is made of transparent PC board.

[0011] As a preferred embodiment of this utility model, the culture chamber is cube-shaped.

[0012] As a preferred embodiment of this utility model, the top of the culture chamber is connected to a snap-on top cover, and a fluororubber sealing ring is provided between the top of the culture chamber and the snap-on top cover.

[0013] As a preferred embodiment of this utility model, the culture chamber is provided with a labyrinth structure sealing pattern on the side near the snap-on top cover.

[0014] The culture chamber employs a multi-layered sealing design, including a labyrinthine interlocking structure, fluororubber sealing rings, and an epoxy composite sealing layer, ensuring the device's long-lasting airtightness under pressure or high-temperature conditions. In a 10kPa positive pressure experiment, the leakage rate is <0.1% / hour, far superior to traditional culture devices, eliminating gas leakage interference.

[0015] As a preferred embodiment of this utility model, the distance between the silicone air injection tube and the soil surface is 5±0.5cm.

[0016] As a preferred embodiment of this utility model, the inner wall of the culture chamber is pre-installed with a temperature probe.

[0017] As a preferred embodiment of this utility model, the temperature probe has a measurement range of −10 to 50°C and an accuracy of ±0.2°C.

[0018] The culture chamber incorporates a high-precision temperature probe, eliminating the need to open the lid for temperature measurement and effectively avoiding data deviations caused by disturbances. It supports subsequent expansion with humidity, conductivity, and other sensing modules, adapting to multi-factor control experimental designs.

[0019] As a preferred embodiment of this utility model, the sealed syringe has a volume of 200 mL and a scale accuracy of 0.5%.

[0020] The beneficial effects of this utility model are as follows:

[0021] This invention relates to an acetylene supply module featuring a miniature pressure reducing valve and a Luer internal thread connector, which connects to a syringe. An external Luer thread connector on the culture chamber also connects to the syringe. The miniature pressure reducing valve lowers the acetylene pressure in the cylinder to a controllable range of 0.01–0.1 MPa, while the Luer interface allows for rapid syringe connection. Users can precisely extract acetylene volumes from μL to mL using the syringe and sequentially open and close the valve to achieve gas sealing and subsequent injection operations, ensuring an operational error of ≤±2% and improving injection efficiency by approximately 300%. This invention solves the problems of difficult acetylene metering and large micro-injection errors in traditional devices, significantly improving experimental repeatability and reliability. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the soil cultivation module;

[0023] Figure 2 This is a schematic diagram of the acetylene supply module;

[0024] Figure 3 This is a schematic diagram of the gas acquisition module.

[0025] In the diagram: 1-Acetylene supply module; 2-Soil culture module; 3-Gas collection module; 4-Temperature probe; 11-Cylinder; 12-Miniature pressure reducing valve; 13-Luer internal thread connector; 14-Injector; 21-Cultivation chamber; 22-Soil; 23-Double-way stopcock valve; 24-Silicone gas injection tube; 25-Luer external thread connector; 26-Snap-on top cap; 27-Fluororubber sealing ring; 31-Luer connector; 32-Stop valve; 33-Sealed injector; 34-Gas collection tube; 35-Gas storage bag. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0027] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention. It should be noted that, unless otherwise specified, the embodiments and features described in the embodiments of the present invention can be combined with each other.

[0028] like Figures 1-3As shown, the nitrous oxide gas collection device with Luer quick-connect acetylene quantitative and high-sealing culture structure in this embodiment includes an acetylene supply module 1, a soil culture module 2, and a gas collection module 3. The acetylene supply module 1 includes a steel cylinder 11 containing acetylene, a miniature pressure reducing valve 12 connected to the cylinder 11, and a Luer internal thread connector 13 connected to the miniature pressure reducing valve 12 via a pipe. The Luer internal thread connector 13 is detachably connected to a syringe 14. The soil culture module 2 includes a culture chamber 21 containing soil 22, and a two-way stopcock valve 23 connected to the culture chamber 21. The two-way stopcock valve 23 is connected to... The gas collection module 3 includes a silicone gas injection tube 24 that extends into the culture chamber 21. The other end of the double-port stopcock valve 23 is connected to a Luer threaded connector 25 for detachable connection to the syringe 14. The gas collection module 3 includes a Luer connector 31. A gas collection port is provided on the culture chamber 21. The Luer connector 31 is connected to the gas collection port. The other end of the Luer connector 31 is connected to a stopcock valve 32 through a pipe. The stopcock valve 32 is detachably connected to a sealed syringe 33. The gas collection module 3 also includes a gas collection tube 34 and a gas storage bag connected to the gas collection tube 34. The other end of the gas collection tube 34 is detachably connected to the sealed syringe 33.

[0029] This invention addresses the problems of imprecise acetylene injection control, unstable sealing performance, complex sampling operations, and lack of monitoring functions in existing technologies. It proposes an integrated experimental device with a compact structure, reliable sealing, precise injection, convenient sampling, and environmental monitoring functions, thereby improving the applicability and accuracy of the acetylene inhibition method in the study of N2O emission mechanisms in soil.

[0030] The acetylene supply module 1 of this invention features a miniature pressure reducing valve 12 and a Luer internal thread connector 13, which connects to a syringe 14. A Luer external thread connector 25 on the culture chamber 21 also connects to the syringe 14. The miniature pressure reducing valve 12 reduces the acetylene pressure in the cylinder 11 to a controllable range of 0.01–0.1 MPa. The Luer interface allows for quick connection of the syringe 14. Users can precisely extract acetylene volumes from μL to mL using the syringe 14, and complete gas sealing and subsequent injection operations by sequentially opening and closing the valve, ensuring an operational error ≤ ±2% and improving injection efficiency by approximately 300%. This invention solves the problems of difficult acetylene metering and large micro-injection errors in traditional devices, significantly improving experimental repeatability and reliability.

[0031] During sampling, the syringe 14 can be connected to the gas sampling port multiple times without contamination. After extracting the top gas sample, it is quantitatively transferred to the gas storage bag through the gas sampling tube 34 for subsequent gas chromatography analysis. The matching high-precision syringe 14 and stopcock valve 32 enable contamination-free, multiple sampling operations, and the gas transfer is completed in a closed loop via a PTFE hose connected to the gas storage bag, greatly improving the integrity of the N2O sample and the accuracy of subsequent analysis.

[0032] Specifically, such as Figure 2 As shown, the acetylene supply module 1 consists of a miniature pressure reducing valve 12, a Luer threaded connector 13, and a standard syringe 14. The miniature pressure reducing valve 12 is used to reduce the acetylene pressure in the cylinder 11 to a controllable range of 0.01–0.1 MPa, and the Luer threaded connector 13 enables quick connection of the syringe 14. Users can accurately extract acetylene volumes from μL to mL using the syringe 14, and complete gas sealing and subsequent injection operations by sequentially opening and closing the valves, ensuring an operational error ≤ ±2%.

[0033] Specifically, such as Figure 1 As shown, soil cultivation module 2 consists of three parts:

[0034] Culture chamber 21: A cubic sealed container (adjustable volume) made of transparent high-strength PC board, with a labyrinth structure sealing pattern on the top, equipped with a fluororubber sealing ring 27 and a snap-on top cover 26, with excellent sealing performance, able to withstand 10kPa pressure, and a leakage rate of <0.1% / h.

[0035] Acetylene injection port: Located in the center of the incubator top cover, it is a Luer external thread connector 25, connecting a two-way stopcock valve 23 and a silicone gas injection tube 24, allowing direct injection of acetylene gas into the surface layer of soil 22. The distance between the silicone gas injection tube 24 and the surface layer of soil 22 is 5±0.5cm.

[0036] Embedded sensor: Temperature probe 4 is pre-installed on the inner wall of the incubator to achieve real-time monitoring of soil environmental parameters and avoid interference from opening the lid. The temperature probe 4 has a measurement range of −10 to 50°C and an accuracy of ±0.2°C.

[0037] The culture chamber 21 employs a multi-layer sealing design, consisting of a labyrinthine interlocking structure, a fluororubber sealing ring 27, and an epoxy composite sealing layer, ensuring the device's long-lasting airtightness under pressure or high temperature conditions. In a 10kPa positive pressure experiment, the leakage rate is <0.1% / hour, far superior to traditional culture devices, eliminating gas leakage interference.

[0038] The culture chamber 21 has an embedded high-precision temperature probe 4, which allows for temperature measurement without opening the lid, effectively avoiding data deviations caused by disturbances. It supports subsequent expansion with humidity, conductivity, and other sensing modules, adapting to multi-factor control experimental designs.

[0039] Specifically, such as Figure 3 As shown, the gas sampling module 3 includes a 200mL sealed syringe 33 with a graduation accuracy of 0.5%, a stopcock valve 32, a polytetrafluoroethylene (PTFE) gas sampling tube 34, and a gas storage bag. During sampling, the syringe 14 can be connected to the gas sampling port multiple times without contamination, and the gas sample from the top is quantitatively transferred to the gas storage bag through the tubing for subsequent gas chromatography analysis.

[0040] Working principle and method:

[0041] Gas preparation and injection: First, the target volume of acetylene is drawn from the outlet of the micro pressure reducing valve 12 through the syringe 14. After sealing, it is connected to the culture chamber 21 and injected precisely into the surface layer of the soil 22 inside the culture chamber 21 through the double-port stopcock valve 23.

[0042] Soil 22 cultivation process: The cultivation chamber 21 maintains the set temperature and humidity conditions, acetylene inhibits the nitrification process, and the accumulation of N2O reflects the denitrification gas production capacity. Dynamic cultivation and timed sampling can be carried out.

[0043] Gas collection and transfer: Headspace gas is extracted using a sealed syringe 33 and quantitatively transferred to a gas storage bag through a gas collection tube 34, supporting repeated collection and subsequent analysis at different time points.

[0044] Compared with existing acetylene inhibition experimental devices, this invention has significant improvements and technical advantages in terms of structural design, ease of operation, functional integration, and experimental accuracy, specifically reflected in the following aspects:

[0045] 1. Achieving precise control and injection of μL-level acetylene gas: By combining a miniature pressure reducing valve 12 with a standard Luer interface injector 14, the problems of difficult acetylene metering and large micro-injection errors in traditional devices are solved. The acetylene operation error is controlled within ±2%, and the injection efficiency is improved by approximately 300%, significantly enhancing experimental repeatability and reliability.

[0046] 2. Significantly improves the airtightness and stability of the soil culture system: The culture chamber 21 adopts a multi-seal design with a labyrinthine interlocking structure, a fluororubber sealing ring 27, and an epoxy composite sealing layer, ensuring the device's long-lasting airtightness under pressure or high temperature conditions. In a 10kPa positive pressure experiment, the leakage rate is <0.1% / hour, far superior to traditional culture devices, eliminating gas leakage interference.

[0047] 3. Supports multiple rounds of efficient gas sampling and sealed transfer during experiments: The matching high-precision syringe 14 and double-port stopcock valve 23 enable pollution-free, multiple sampling operations, and complete the sealed gas transfer by connecting the gas storage bag through a PTFE hose, which greatly improves the integrity of N2O samples and the accuracy of subsequent analysis.

[0048] 4. Features embedded real-time environmental parameter monitoring: The device incorporates a high-precision temperature probe 4, eliminating the need to open the cover for temperature measurement and effectively avoiding data deviations caused by disturbances. It supports subsequent expansion with humidity, conductivity, and other sensing modules, adapting to multi-factor control experimental designs.

[0049] 5. Modular structure design enhances system scalability and field adaptability: Each core component adopts a standard Luer interface connection, which facilitates quick assembly and replacement. It is suitable for various application scenarios such as indoor bench experiments and in-situ field measurements, and has good compatibility and promising prospects for technology promotion.

[0050] In summary, this invention not only solves the core problems of inaccurate acetylene injection, poor sealing, low gas extraction efficiency, and large environmental disturbance in the prior art, but also constructs a scalable, repeatable, and highly adaptable experimental platform for the N2O production mechanism in soil through system structure and functional integration innovation, significantly improving the application efficiency and technical level of the acetylene inhibition method in nitrogen cycle research.

[0051] Example:

[0052] I. Device Structure Composition:

[0053] This utility model includes three core modules: acetylene supply module 1, soil cultivation module 2, and gas collection module 3, with the specific structure as follows.

[0054] 1. Acetylene supply module 1 (e.g.) Figure 2 (as shown)

[0055] This module is used to achieve high-precision control and injection of acetylene gas, and mainly consists of the following structure:

[0056] Portable acetylene cylinder 11: Provides a stable acetylene gas source, with a miniature pressure reducing valve 12 connected to the cylinder opening.

[0057] Miniature pressure reducing valve 12: controls the output air pressure within the range of 0.01 to 0.1 MPa to ensure safe operation of small quantities.

[0058] Luer internal thread connector 13: connects to syringe 14 to achieve quick connection and sealing operation.

[0059] 200mL syringe 14 (with graduation): used to draw acetylene with an accuracy of 0.5%; the syringe 14 is equipped with a two-way stopcock valve 23 at the front end, which can control the air intake and closure states.

[0060] In the above structure, the miniature pressure reducing valve 12 and the Luer internal thread connector 13 are essential components to ensure safety and airtightness; the acetylene cylinder specifications are optional, and it can be replaced with a compressed acetylene tank or a gas collection bag.

[0061] 2. Soil cultivation module 2 (e.g.) Figure 1 (as shown)

[0062] This module is used to achieve a closed-loop reaction between acetylene gas and soil sample 22, while maintaining a stable experimental environment.

[0063] Sealed transparent culture chamber 21: The entire chamber is made of high-transmittance PC board with an open top structure and adjustable internal volume (usually 0.5-2L). The top edge is equipped with a labyrinth-style raised sealing strip, which, together with the fluororubber ring and the snap-lock top cover, forms a triple sealing system to ensure no gas leakage within 10kPa.

[0064] Acetylene injection channel: Located in the middle of the top cover, it is connected to the double-way plug valve 23 using a Luer external thread connector 25. The lower end is a corrosion-resistant silicone hose, which is inserted into the soil sample 22 at a depth of 5±0.5cm to ensure that acetylene diffuses rapidly into the reaction area.

[0065] Embedded temperature sensor: fixed to the inner wall or under the top cover of the culture chamber, supporting real-time data recording (range −10~50℃, error ±0.2℃).

[0066] The culture chamber 21 and the gas injection interface are essential structures, while the temperature probe 4 is an optional expansion unit. Modules such as humidity and conductivity can also be added to meet the needs of multi-factor monitoring.

[0067] 3. Gas acquisition module 3 (e.g.) Figure 3 (as shown)

[0068] This module is used to extract N2O gas samples from culture chamber 21 and transfer them to the gas analysis system.

[0069] High-airtightness syringe 14: The 200mL syringe 14 is equipped with a stopcock valve 32 at the front end, which is connected to a polytetrafluoroethylene sampling tube.

[0070] Gas sampling port: Located on the side wall or top cover of the culture chamber 21, connected to Luer connector 31 to ensure a closed and leak-free sampling process.

[0071] Transfer system: The sealed syringe 33 injects the sample into the gas storage bag (such as an aluminum foil bag or a multilayer membrane gas bag) through the gas sampling tube 34 for subsequent gas chromatography and other analyses.

[0072] The sealed syringe 33, sampling valve and gas storage device are essential structures; if conditions permit, a negative pressure pump or multi-channel sampler can be selected to realize automated sampling.

[0073] II. Operating Procedures:

[0074] 1. Preparation stage:

[0075] Soil sample 22 was placed at the bottom of the incubator;

[0076] Close the snap-on top cover 26, lock it in place, and confirm that the sealing strip is tight and there is no air leakage;

[0077] Turn on temperature probe 4 and calibrate the required experimental conditions.

[0078] 2. Acetylene injection:

[0079] Connect the syringe 14 to the miniature pressure reducing valve 12 via the Luer internal thread connector 13;

[0080] Open the acetylene cylinder main valve, miniature pressure reducing valve 12, syringe 14 stopcock valve 32 in sequence;

[0081] Slowly extract a set dose (e.g., 10 mL) of acetylene gas;

[0082] Close the syringe valve 14 and the pressure reduction system in sequence;

[0083] Connect syringe 14 to the acetylene injection port of the incubator and open the double-pass stopcock valve 23 to inject gas.

[0084] After the gas injection is complete, close the interface valve, and the incubator enters the closed culture stage.

[0085] 3. Gas sampling:

[0086] After the culture time is set (e.g., 1h, 3h, 6h, etc.), connect the sealed syringe 33 to the gas sampling port;

[0087] Slowly extract 200 mL of gas and close the stopcock valve 32;

[0088] Connect the sampling tube and transfer it to a gas storage bag for sealed storage;

[0089] Repeat the above steps to perform multi-time point sampling.

[0090] III. Description of Optional Extended Structures:

[0091] Table 1. Optional extended structure table.

[0092]

[0093] Through the above implementation methods, this invention not only improves the operational accuracy and system reliability of the acetylene inhibition experiment, but also provides a stable, efficient, and scalable standardized platform for the study of N2O flux in soil 22.

[0094] This utility model is not limited to the above-mentioned optional embodiments. Anyone can derive other forms of products under the guidance of this utility model. However, regardless of any changes made in its shape or structure, any technical solution that falls within the scope of the claims of this utility model shall be protected by this utility model.

Claims

1. A nitrous oxide gas collection device with a Luer fast-connection acetylene quantitative and highly sealed culture structure, characterized in that: The system includes an acetylene supply module (1), a soil cultivation module (2), and a gas collection module (3). The acetylene supply module (1) includes a steel cylinder (11) containing acetylene. A miniature pressure reducing valve (12) is connected to the steel cylinder (11). The miniature pressure reducing valve (12) is connected to a Luer internal thread connector (13) via a pipe. The Luer internal thread connector (13) is detachably connected to a syringe (14). The soil cultivation module (2) includes a cultivation chamber (21) containing soil (22). A two-way stopcock valve (23) is connected to the cultivation chamber (21). The two-way stopcock valve (23) is connected to a silicone gas injection tube (24). The silicone gas injection tube (24) extends into the cultivation chamber (21). The other end of the two-way stopcock valve (23) is connected to a Luer external thread connector (25) for detachably connecting the syringe (14).

2. The nitrous oxide gas collection device with Luer fast-connection acetylene quantitative and highly sealed culture structure according to claim 1, characterized in that: The gas collection module (3) includes a Luer connector (31), a gas collection port is provided on the culture chamber (21), the Luer connector (31) is connected to the gas collection port, and the other end of the Luer connector (31) is connected to a stopcock valve (32) through a pipe. The stopcock valve (32) is detachably connected to a sealing syringe (33). The gas collection module (3) also includes a gas collection pipe (34) and a gas storage bag connected to the gas collection pipe (34). The other end of the gas collection pipe (34) is detachably connected to the sealing syringe (33).

3. The nitrous oxide gas collection device with Luer fast-connection acetylene quantitative and highly sealed culture structure according to claim 1, characterized in that: The culture chamber (21) is made of transparent PC board.

4. The nitrous oxide gas collection device with Luer fast-connection acetylene quantitative and highly sealed culture structure according to claim 1, characterized in that: The culture chamber (21) is cube-shaped.

5. The nitrous oxide gas collection device with Luer fast-connection acetylene quantitative and highly sealed culture structure according to claim 1, characterized in that: The top of the culture chamber (21) is connected to a snap-on top cover (26), and a fluororubber sealing ring (27) is provided between the top of the culture chamber (21) and the snap-on top cover (26).

6. The nitrous oxide gas collection device with Luer fast-connection acetylene quantitative and highly sealed culture structure according to claim 5, characterized in that: The culture chamber (21) has a labyrinth-structured sealing pattern on the side near the snap-on top cover (26).

7. The nitrous oxide gas collection device with Luer fast-connection acetylene quantitative and highly sealed culture structure according to claim 1, characterized in that: The distance between the silicone gas injection tube (24) and the surface of the soil (22) is 5 ± 0.5 cm.

8. The nitrous oxide gas collection device with Luer fast-connection acetylene quantitative and highly sealed culture structure according to claim 1, characterized in that: The inner wall of the culture chamber (21) is pre-installed with a temperature probe (4).

9. A nitrous oxide gas collection device with a Luer fast-connection acetylene quantitative and highly sealed culture structure according to claim 8, characterized in that: The temperature probe (4) has a measurement range of −10 to 50°C and an accuracy of ±0.2°C.

10. A nitrous oxide gas collection device with a Luer fast-connection acetylene quantitative and highly sealed culture structure according to any one of claims 1 to 9, characterized in that: The sealed syringe (33) has a volume range of 200 mL and a scale accuracy of 0.5%.

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