Human stem cell culture instrument

Abstract

The utility model relates to cell culture technical field, concretely is a kind of human stem cell culture instrument, including incubator, the inside of the incubator is provided with several layers of tray, the tray is equipped with breathable hole, the side surface of the incubator is fixedly connected with air inlet pipe, the end of air inlet pipe away from incubator is fixedly connected with purification tank. In the utility model, by setting up air inlet pipe connection purification tank in the side surface of incubator, there is air filter unit in purification tank, can effectively filter the gas entering incubator, remove dust particles, microorganism and other impurities, guarantee the purity and sterility of gas entering incubator inside, create stable and suitable gas environment for stem cell culture, conducive to normal growth, proliferation and maintaining its characteristics of stem cell, simultaneously, by fixed assembly, it is also convenient to dismount the bearing frame in purification tank, it is convenient to replace the air filter unit in bearing frame in later period.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a human stem cell culture instrument. Background Technology

[0002] In the in vitro culture of human stem cells, creating a stable, sterile, and suitable growth environment is crucial. The concentrations of gases in this environment, such as oxygen and carbon dioxide, need precise control, and the purity of these gases directly affects the quality of stem cell culture. Currently, conventional stem cell culture instruments often lack effective air filtration mechanisms in their gas supply. Dust particles, microorganisms, and other impurities from the outside air can easily enter the culture chamber with the gas, potentially contaminating the stem cell culture medium, interfering with the normal growth, proliferation, and maintenance of stem cell characteristics, leading to culture failure or affecting the effectiveness of subsequent research and clinical applications. Utility Model Content

[0003] The purpose of this invention is to solve the problems mentioned in the background art and to propose a human stem cell culture instrument.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: a human stem cell culture instrument, comprising a culture chamber, wherein the culture chamber has several layers of trays inside, the trays having ventilation holes, an air inlet pipe fixedly connected to the side of the culture chamber, a purification chamber fixedly connected to the end of the air inlet pipe away from the culture chamber, a gas supply pipe fixedly connected to the side of the purification chamber away from the air inlet pipe, an air outlet pipe fixedly connected to the other side of the culture chamber, an electrically controlled valve provided on the air outlet pipe, a flow regulating valve provided on the air inlet pipe, a controller fixedly connected to the side of the culture chamber fixedly connected to the air inlet pipe, a square groove provided on the top of the purification chamber, a slot provided at the bottom of the square groove, a support frame inserted inside the slot, an air filter unit provided inside the support frame, a slidably connected cover plate provided on the top of the support frame, the cover plate being secured inside the square groove, and the support frame being fixedly connected to the purification chamber through the cover plate and two sets of fixing components.

[0005] Preferably, the fixing component includes a fixing block, which is fixedly connected to the side of the purification box. The fixing block is internally threaded with a lead screw, and a knob is fixedly connected to the top of the lead screw. A locking block is rotatably connected to the lead screw and is locked onto the cover plate.

[0006] Preferably, the air filtration unit includes a pre-filter layer, a medium-efficiency filter layer and a high-efficiency filter layer in sequence along the gas inlet direction. The pre-filter layer is made of fiber filter material with large pore size, the medium-efficiency filter layer is made of material composed of activated carbon fiber and polypropylene fiber, and the high-efficiency filter layer is made of high-efficiency air filter (HEPA) material.

[0007] Preferably, a support rod is fixedly connected to the inner wall of the incubator, and a groove is provided on the support rod at the position corresponding to the edge of the tray, and the two side edges of the tray are slidably connected to the inside of the groove.

[0008] Preferably, the incubator has a door on the front, and a transparent observation window is embedded in the door.

[0009] Preferably, a sealing ring is provided inside the square groove, and the sealing ring is made of elastic rubber.

[0010] Preferably, a limiting rod is fixedly connected to the bottom of the card block, and a limiting groove is formed on the cover plate at the position corresponding to the limiting rod, with the limiting rod inserted inside the limiting groove.

[0011] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0012] 1. In this utility model, an air inlet pipe is set on the side of the incubator to connect to a purification box. The purification box has an air filter unit that can effectively filter the gas entering the incubator, remove dust particles, microorganisms and other impurities, and ensure the purity and sterility of the gas entering the incubator. This creates a stable and suitable gas environment for stem cell culture, which is conducive to the normal growth, proliferation and maintenance of stem cells. At the same time, the fixing components also make it easy to disassemble and assemble the carrier frame inside the purification box, which is convenient for the air filter unit inside the carrier frame to be replaced later.

[0013] 2. In this utility model, the limiting rod at the bottom of the card block is inserted into the limiting groove of the cover plate. This structural design enhances the connection stability between the card block and the cover plate, making the fixing component more secure and reliable when fixing the carrier frame. It avoids the card block from loosening due to vibration and other factors during the operation of the incubator, which would affect the fixing effect between the carrier frame and the purification box. Attached Figure Description

[0014] Figure 1 A schematic diagram of a human stem cell culture device is provided for this utility model;

[0015] Figure 2 An exploded view of a human stem cell culture instrument is provided for this utility model.

[0016] Figure 3An exploded view of the purification chamber of a human stem cell culture instrument is provided for this utility model.

[0017] Figure 4 This invention provides a partially exploded view of a human stem cell culture device.

[0018] Figure 5 This invention provides a cross-sectional view of the air purification unit of a human stem cell culture device.

[0019] Legend:

[0020] 1. Incubator; 2. Door; 3. Observation window; 4. Tray; 5. Support rod; 6. Slide groove; 7. Vent hole; 8. Controller; 9. Gas supply pipe; 10. Purification chamber; 11. Air inlet pipe; 12. Flow regulating valve; 13. Fixing assembly; 131. Fixing block; 132. Lead screw; 133. Knob; 134. Locking block; 14. Groove; 15. Square groove; 16. Sealing ring; 17. Bearing frame; 18. Cover plate; 19. Air filtration unit; 191. Primary filter layer; 192. Medium-efficiency filter layer; 193. High-efficiency filter layer; 20. Limiting rod; 21. Limiting groove; 22. Air outlet pipe; 23. Electrically controlled valve. Detailed Implementation

[0021] To better understand the above-mentioned objectives, features, and advantages of this utility model, the present utility model will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0022] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed in the following specification.

[0023] Example 1: As Figure 1 - Figure 5As shown, this utility model provides a technical solution: a human stem cell culture instrument, including a culture chamber 1, with several layers of trays 4 inside the culture chamber 1, and ventilation holes 7 on the trays 4. An air inlet pipe 11 is fixedly connected to the side of the culture chamber 1, and a purification chamber 10 is fixedly connected to the end of the air inlet pipe 11 away from the culture chamber 1. A gas supply pipe 9 is fixedly connected to the side of the purification chamber 10 away from the air inlet pipe 11, and an air outlet pipe 22 is fixedly connected to the other side of the culture chamber 1. An electrically controlled valve 23 is installed on the air outlet pipe 22. A flow regulating valve 12 is installed on the air inlet pipe 11. A controller 8 is fixedly connected to one side of the incubator 1 where the air inlet pipe 11 is fixedly connected. A square groove 15 is opened on the top of the purification chamber 10, and a slot 14 is opened at the bottom of the square groove 15. A support frame 17 is inserted into the slot 14, and an air filter unit 19 is installed inside the support frame 17. A slidably connected cover plate 18 is installed on the top of the support frame 17. The cover plate 18 is locked inside the square groove 15, and the support frame 17 is secured by the cover plate 18 and two sets of fixing components. The fixing assembly 13 is fixedly connected to the purification box 10. The fixing assembly 13 includes a fixing block 131, which is fixedly connected to the side of the purification box 10. A screw rod 132 is threadedly connected to the inside of the fixing block 131. A knob 133 is fixedly connected to the top of the screw rod 132. A locking block 134 is rotatably connected to the screw rod 132 and is locked onto the cover plate 18. The air filter unit 19 includes a pre-filter layer 191, a medium-efficiency filter layer 192, and a high-efficiency filter layer 193 in sequence along the gas inlet direction. The high-efficiency filter layer 191 is made of fiber filter material with large pore size, the medium-efficiency filter layer 192 is made of material composed of activated carbon fiber and polypropylene fiber, and the high-efficiency filter layer 193 is made of high-efficiency air filter (HEPA) material. The inner wall of the incubator 1 is fixedly connected to the support rod 5, and the support rod 5 is provided with a groove 6 at the position corresponding to the edge of the tray 4. The two sides of the tray 4 are slidably connected to the inside of the groove 6. The front of the incubator 1 is provided with a door 2, and a transparent observation window 3 is embedded in the door 2.

[0024] In this embodiment, an air inlet pipe 11 is provided on the side of the incubator 1 to connect to the purification box 10. The purification box 10 contains an air filter unit 19, which can effectively filter the gas entering the incubator 1, removing dust particles, microorganisms and other impurities, ensuring the purity and sterility of the gas entering the incubator 1, creating a stable and suitable gas environment for stem cell culture, which is conducive to the normal growth, proliferation and maintenance of stem cells. At the same time, by rotating the knob 133 of the fixing component 13, the lead screw 132 is driven to rotate, so that the locking block 134 is locked on the cover plate 18, thereby fixing the support frame 17. The operation is simple and convenient, and it is convenient to quickly disassemble and install the support frame 17 when the air filter unit 19 needs to be replaced or maintained. It is also convenient to regularly inspect, clean or replace the filter unit, ensuring the effectiveness of the air filtration function, thereby continuously maintaining a good gas filtration effect and ensuring the quality of the gas entering the incubator 1. The air filter unit 19 is composed of a primary filter layer 191, a medium-efficiency filter layer 192 and a high-efficiency filter layer 193, forming a layered filtration system. The primary filter layer 191 intercepts large-diameter dust particles and other impurities, reducing the burden on subsequent filter layers; the secondary filter layer 192 adsorbs odors, some organic pollutants, and smaller particles, further purifying the air; the high-efficiency filter layer 193 filters out bacteria, viruses, and other microorganisms to the maximum extent, ensuring that the gas entering the incubator 1 is nearly sterile. This multi-layer filtration structure greatly improves the purity of the gas, effectively avoiding interference from external air pollution on stem cell culture and creating an ideal growth environment for stem cells; the slide groove 6 on the inner wall support rod 5 of the incubator 1 is slidably connected to the edge of the tray 4, allowing the culture dish to be placed on the tray 4 by sliding the tray 4 from... Pulling the incubator 1 outwards so that most of the tray 4 is outside the incubator 1 improves the convenience of placing the culture dish in the groove on the tray 4, making it easier to remove the culture dish from the incubator 1 later. By setting a door 2 with a transparent observation window 3 on the front of the incubator 1, the operator can observe the culture status of stem cells inside the incubator 1 in real time without opening the door 2, such as observing the growth morphology of cells, the color and volume of the culture medium, etc. This avoids the risk of contamination caused by the entry of outside air due to frequent door opening, and also improves the efficiency of daily observation, which helps to detect abnormalities in the culture process in a timely manner and take corresponding measures.

[0025] Example 2: Figure 3 As shown, a sealing ring 16 is provided inside the square groove 15. The sealing ring 16 is made of elastic rubber. A limiting rod 20 is fixedly connected to the bottom of the locking block 134. A limiting groove 21 is opened on the cover plate 18 at the position corresponding to the limiting rod 20. The limiting rod 20 is inserted into the limiting groove 21.

[0026] In this embodiment, an elastic rubber sealing ring 16 is provided in the square groove 15. When the support frame 17 is installed in conjunction with the square groove 15 through the cover plate 18, the sealing ring 16 can fit tightly and fill the gap between the two, effectively preventing gas leakage from the connection between the support frame 17 and the purification box 10. The limiting rod 20 at the bottom of the locking block 134 is inserted into the limiting groove 21 of the cover plate 18. This structural design enhances the connection stability between the locking block 134 and the cover plate 18, making the fixing component 13 more secure and reliable when fixing the support frame 17. This avoids the locking block 134 from loosening due to vibration or other factors during the operation of the incubator, which would affect the fixing effect between the support frame 17 and the purification box 10.

[0027] The working principle of this embodiment is as follows: In use, first open the door 2 of the incubator 1, then pull the tray 4 outwards. The two sides of the tray 4 slide within the grooves 6 on the corresponding support rods 5. Then place the culture dish in the groove on the tray 4. Repeat this process for the remaining trays 4. Finally, push the tray 4 into the incubator 1 and close the door 2. When the human stem cell culture instrument is working, outside air first enters the purification chamber 10 through the gas supply pipe 9. An air filtration unit 19 is installed inside the support frame 17 of the purification chamber 10. The air filtration unit 19 consists of a primary filter layer 191, a medium-efficiency filter layer 192, and a high-efficiency filter layer 193, arranged sequentially according to the gas entry direction. When air enters, it first passes through the primary filter layer 191. The high-efficiency filter layer 191 intercepts large-particle impurities such as dust and hair in the air, completing the initial purification of the air and reducing the burden on subsequent filter layers. The pre-purified air then enters the medium-efficiency filter layer 192, which adsorbs odors, some organic pollutants, and fine particles, further improving air cleanliness. The air then passes through the medium-efficiency filter layer 193, which filters out bacteria, viruses, and other tiny microorganisms to the maximum extent, ensuring that the gas entering the incubator 1 is almost sterile and pure. The pure gas filtered by the purification chamber 10 enters the incubator 1 through the inlet pipe 11, which is equipped with a flow regulating valve 12 to adjust the flow rate according to the dryness and fineness of the air inside the incubator 1. The required gas concentration for cell culture is precisely controlled by the flow regulating valve 12, regulated by the controller 8. The gas entering the incubator 1 is evenly distributed inside through the vents 7 on the tray 4, providing a suitable gaseous environment for the stem cell culture samples placed on the tray 4. During culture, stem cells consume oxygen and produce metabolic gases such as carbon dioxide. The exhaust pipe 22 on the other side of the incubator 1 can expel these excess gases. The electrically controlled valve 23 on the exhaust pipe 22 can open or close in a timely manner based on the gas concentration monitoring inside the incubator 1 (which can be fed back to the controller 8 via external sensors, and the controller 8 determines and controls the process), maintaining a stable gas concentration and internal pressure balance within the incubator 1. Various sensors installed inside chamber 1, such as oxygen sensors, carbon dioxide sensors, temperature sensors, and humidity sensors (not shown in the figure, but these sensors are present in actual applications), monitor various parameters of the culture environment in real time. If any parameter deviates from the preset range, the controller 8 promptly activates the corresponding adjustment mechanism, such as adjusting heating, cooling, humidification, dehumidification devices, and gas flow regulation devices (not shown in the figure, but these sensors are present in actual applications), ensuring that the entire environment inside chamber 1 remains in a stable state optimal for stem cell culture. When maintenance or replacement of the air filter unit 19 is required, it can be operated through the fixing component 13. First, turn the knob 133...The screw 132 rotates within the fixed block 131, causing the locking block 134 to move upwards, disengaging it from the cover plate 18. Then, the handle on the cover plate 18 is grasped to pull the carrier frame 17 out of the slot 14. The cover plate 18 is then separated from the carrier frame 17, allowing the air filter unit 19 to be removed from the carrier frame 17 for replacement or cleaning. After replacing the air filter unit 19, the carrier frame 17 is reinstalled in reverse. The limiting rod 20 on the locking block 134 is inserted into the limiting groove 21 on the cover plate 18, improving the stability of the locking block 134's fixation on the cover plate 18 and preventing rotation due to vibration or other reasons, which could affect the fixation effect. The incubator 1 has a door 2 with an observation window 3 on the front, allowing operators to easily observe the internal stem cell culture process while reducing unnecessary door opening operations, lowering the risk of external air contamination of the culture environment, and ensuring that stem cell culture proceeds smoothly in a stable, sterile, and suitable environment.

[0028] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present utility model without departing from the technical solution of the present utility model shall still fall within the protection scope of the technical solution of the present utility model.

Claims

1. A human stem cell culture instrument, comprising an incubator (1), characterized in that: The incubator (1) has several layers of trays (4) inside, and each tray (4) has ventilation holes (7). An air inlet pipe (11) is fixedly connected to the side of the incubator (1). A purification chamber (10) is fixedly connected to the end of the air inlet pipe (11) away from the incubator (1). A gas supply pipe (9) is fixedly connected to the side of the purification chamber (10) away from the air inlet pipe (11). An air outlet pipe (22) is fixedly connected to the other side of the incubator (1). An electric control valve (23) is installed on the air outlet pipe (22). A flow regulating valve (12) is installed on the air inlet pipe (11). A controller (8) is fixedly connected to one side of the air inlet pipe (11). A square groove (15) is opened on the top of the purification box (10). A slot (14) is opened at the bottom of the square groove (15). A support frame (17) is inserted inside the slot (14). An air filter unit (19) is installed inside the support frame (17). A cover plate (18) is slidably connected to the top of the support frame (17). The cover plate (18) is locked inside the square groove (15). The support frame (17) is fixedly connected to the purification box (10) through the cover plate (18) and two sets of fixing components (13).

2. The human stem cell culture instrument according to claim 1, characterized in that: The fixing component (13) includes a fixing block (131), which is fixedly connected to the side of the purification box (10). The fixing block (131) is internally threaded with a screw rod (132), and a knob (133) is fixedly connected to the top of the screw rod (132). A locking block (134) is rotatably connected to the screw rod (132), and the locking block (134) is locked onto the cover plate (18).

3. The human stem cell culture instrument according to claim 1, characterized in that: The air filtration unit (19) includes a primary filter layer (191), a secondary filter layer (192), and a high-efficiency filter layer (193) in sequence along the gas inlet direction. The primary filter layer (191) is made of fiber mesh material, the secondary filter layer (192) is made of a composite material of activated carbon fiber and polypropylene fiber, and the high-efficiency filter layer (193) is made of high-efficiency air filter material.

4. The human stem cell culture instrument according to claim 1, characterized in that: The inner wall of the incubator (1) is fixedly connected to a support rod (5), and the support rod (5) is provided with a groove (6) at the position corresponding to the edge of the tray (4). The two sides of the tray (4) are slidably connected to the inside of the groove (6).

5. The human stem cell culture instrument according to claim 1, characterized in that: The incubator (1) has a door (2) on the front, and a transparent observation window (3) is embedded in the door (2).

6. The human stem cell culture instrument according to claim 1, characterized in that: The square groove (15) is provided with a sealing ring (16), which is made of elastic rubber.

7. The human stem cell culture instrument according to claim 2, characterized in that: The bottom of the card block (134) is fixedly connected to a limiting rod (20), and a limiting groove (21) is opened on the cover plate (18) at the position corresponding to the limiting rod (20), and the limiting rod (20) is inserted into the limiting groove (21).

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