A nucleic acid extraction reagent strip and system

Abstract

The utility model belongs to the technical field of medical apparatus and instruments, concretely relates to a nucleic acid extraction reagent strip and system, a nucleic acid extraction reagent strip, including reagent strip main part, reagent strip main part is by the reagent end of one end and the consumable end of the other end detachable connection constitutes, and the both sides of the connecting place of reagent end and consumable end are equipped with the sliding slot, and the both sides of the connecting place of consumable end and reagent end are equipped with the guide slide, and the guide slide is used for inserting the sliding slot of reagent end, and consumable end and reagent end are detachably connected, the utility model makes nucleic acid extraction reagent strip can realize first encapsulation reagent again installation consumable, and processing is convenient, and flexible collocation, and also can avoid consumable pollution, reduce the pollution risk, improve the practicality and efficiency of reagent strip.

Description

Technical Field

[0001] This utility model belongs to the field of medical device technology, specifically relating to a nucleic acid extraction reagent strip and system. Background Technology

[0002] Sample processing is the "first step" in molecular diagnostic experiments, and it is also a crucial step, as the quality of the nucleic acids obtained directly affects the success or failure of downstream molecular biology experiments.

[0003] Nucleic acid extraction reagent strips used in the nucleic acid purification process are disposable consumables used to obtain purified products. They can accommodate various reaction reagents, provide reaction space, and meet the requirements for sample protection during the nucleic acid extraction and purification process.

[0004] The magnetic bead method features magnetic beads with unique separation properties and a unique buffer system. Under certain conditions, magnetic beads have a strong affinity for nucleic acids. When the conditions change and an external magnetic field is applied, the nucleic acids attach to the magnetic beads and move in a specific direction, thereby achieving the purpose of separating nucleic acids from other substances. Nucleic acid extraction using the magnetic bead method can generally be divided into four steps: lysis, binding, washing, and elution.

[0005] Commercially available nucleic acid extraction reagent strips are generally one-piece units, suitable for use with matching nucleic acid extraction equipment. However, they are incompatible when changing nucleic acid extraction equipment or consumables, which limits the experiment. Therefore, there is a need for a nucleic acid extraction reagent strip that can be flexibly combined. Utility Model Content

[0006] The problem this invention aims to solve is to provide a nucleic acid extraction reagent strip that is easy to handle and can be flexibly combined when changing nucleic acid extraction equipment or consumables.

[0007] To solve the above-mentioned technical problems, this utility model provides the following technical solution: Firstly, This utility model provides a nucleic acid extraction reagent strip, including a reagent strip body. The reagent strip body is composed of a reagent end at one end and a consumable end at the other end, which are detachably connected. Slide grooves are provided on both sides of the connection between the reagent end and the consumable end, and guide slides are provided on both sides of the connection between the consumable end and the reagent end. The guide slides are used to insert into the slide groove of the reagent end to detachably connect the consumable end and the reagent end.

[0008] Furthermore, a buckle is provided at the connection between the consumable end and the reagent end, and the buckle is located between the guide slides on both sides; a buckle fixing position is provided at the bottom of the slide groove of the reagent end, and the top of the slide groove is set as the upper limit position of the slide; when the consumable end and the reagent end are connected, the guide slide is inserted into the slide groove, and the top of the guide slide abuts against the bottom of the upper limit position of the slide, and the bottom of the buckle is locked on the buckle fixing position.

[0009] Furthermore, the reagent end is equipped with multiple reagent wells, which are used to store various reagents required for nucleic acid extraction; each reagent well is uniformly sealed with a membrane.

[0010] Furthermore, from the reagent end to the consumable end, there are sequentially arranged pyrolysis wells, idle well one, cleaning well one, cleaning well two, idle well two, magnetic bead preparation / recovery wells, and elution wells.

[0011] Furthermore, the lysis well, idle well one, cleaning well one, cleaning well two, idle well two, magnetic bead preparation / recovery well, and elution well are all reagent wells.

[0012] Furthermore, the consumable end is provided with a magnetic rod sleeve hole and multiple tip suction head holes in sequence.

[0013] Furthermore, there are two tip nozzle holes, including tip nozzle hole two and tip nozzle hole one.

[0014] Secondly, This utility model provides a nucleic acid extraction reagent system, including a reagent strip holder and the aforementioned nucleic acid extraction reagent strip that is movably installed on the reagent strip holder; a reinforcing rib that also functions as a slide rail is provided below the reagent end, so that the reagent strip body can slide when installed on the reagent strip holder.

[0015] Furthermore, a fixing head is provided at one end of the tray of the reagent strip holder, and a fixing head is provided below the reagent end. The reagent strip body can be connected to the fixing head by means of the fixing head.

[0016] Furthermore, the reagent strip holder has raised fixing positions on both sides of the middle of the tray, and raised positions on both sides of the other end of the reagent strip. The reagent strip body can be connected to the raised fixing positions through the raised positions.

[0017] Compared with the prior art, the present invention has the following beneficial effects: This invention provides a nucleic acid extraction reagent strip. The main body of the reagent strip is composed of a reagent end at one end and a consumable end at the other end, which can be detachably connected. This allows for the pre-packaging of reagents and subsequent installation of consumables, making the process convenient and flexible. It also avoids consumable contamination and reduces the risk of contamination, thereby improving the practicality and efficiency of the reagent strip.

[0018] This novel nucleic acid extraction reagent strip uses a snap-fit ​​assembly method, which allows for different production processes such as simultaneous packaging of reagents and consumables followed by reassembly, or packaging reagents first and then consumables after assembly; thus improving the practicality and efficiency of the reagent strip.

[0019] The nucleic acid extraction reagent strip provided by this utility model has seven sets of reagent wells, containing a variety of reagents required for nucleic acid extraction. The main body of the reagent strip is assembled from two parts, one end of which is the reagent end and the other end is the consumable end. The reagent is first encapsulated and then the consumable is installed, allowing for flexible matching and avoiding contamination of the consumable.

[0020] The nucleic acid extraction reagent strip provided by this utility model can provide the reagents and consumables required for nucleic acid extraction, and ultimately serve as a waste disposal site, making it easy to handle; the reagent is manually sealed first, and then the tip is installed to avoid contamination, and it is a disposable consumable that does not require cleaning or replacement; it can prevent reagents from dripping onto the instrument or cross-contamination between reagents, making it safe and effective.

[0021] The nucleic acid extraction reagent strip provided by this utility model has a reinforcing rib for support and to act as a slide rail; the protrusion is used to fix the reagent strip to the reagent strip holder, making it easy to install to the reagent strip holder. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of the nucleic acid extraction reagent strip provided by this utility model.

[0023] Figure 2 This is a schematic diagram of the combined structure of the nucleic acid extraction reagent strip provided by this utility model, showing the consumable end side.

[0024] Figure 3 This is a schematic diagram of the combined structure of the nucleic acid extraction reagent strip provided by this utility model, showing the reagent end side.

[0025] Figure 4 This is a magnified view of the connection between the reagent end and the consumable end of the nucleic acid extraction reagent strip provided by this utility model after disassembly.

[0026] Figure 5 This is a schematic diagram of the nucleic acid extraction reagent system provided by this utility model.

[0027] Figure label: 100 - Reagent end; 101 - Elution well; 102 - Magnetic bead preparation / recovery well; 103 - Second available well; 104 - Second cleaning well; 105 - First cleaning well; 106 - First available well; 107 - Pyrolysis well; 108 - Fixing head; 109 - Reinforcing rib; 110 - Protrusion; 111 - Slide groove; 112 - Snap-fit ​​fixing position; 113 - Upper limit of slide bar; 200 - Consumable end; 201 - Tip nozzle hole position one; 202 - Tip nozzle hole position two; 203 - Magnetic rod sleeve hole; 204 - Buckle; 205 - Guide slide bar; 300 - Reagent strip holder; 301 - Protruding fixing position; 302 - Fixing head fixing position. Detailed Implementation

[0028] The technical solution of this utility model will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are not all embodiments of this utility model. All other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0029] It should be noted that the terms "center", "upper", "lower", "horizontal", "left", "right", "front", "back", "lateral", "longitudinal", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0030] Example 1 like Figures 1-4 As shown, this utility model provides a nucleic acid extraction reagent strip, which includes a reagent strip body. The reagent strip body is composed of a reagent end 100 at one end and a consumable end 200 at the other end that are detachably connected.

[0031] The reagent end 100 is provided with the following in sequence from the consumable end 200: pyrolysis well 107, idle well 106, cleaning well 105, cleaning well 104, idle well 103, magnetic bead preparation / recovery well 102, and elution well 101. Lysis well 107, idle well 106, washing well 105, washing well 2 104, idle well 2 103, magnetic bead preparation / recovery well 102, and elution well 101 are all reagent wells. There are seven reagent wells, which are used to store various reagents required for nucleic acid extraction.

[0032] Starting from the connection point with the reagent end 100, the consumable end 200 is sequentially provided with a magnetic rod sleeve hole 203, a second tip pipette hole 202, and a first tip pipette hole 201. There are two tip pipette holes, which are used to hold ready-to-use or used discarded pipette tips. The magnetic rod sleeve hole 203 is used to hold ready-to-use or used discarded magnetic rod sleeves; the magnetic rod sleeve is used to attract magnetic beads and for stirring.

[0033] The reagent end 100 and the consumable end 200 are connected by sliding grooves 111 on both sides, which are used to install the consumable end 200 to the reagent end 100; the consumable end 200 and the reagent end 100 are connected by guide strips 205 on both sides, which are used to insert into the sliding grooves 111 of the reagent end 100, and serve to connect the consumable end 200 and the reagent end 100.

[0034] A snap-fit ​​fixing position 112 is provided at the bottom between the slide grooves 111 of the reagent end 100, and the top of the slide grooves 111 is provided as the upper limit position of the slide bar 113.

[0035] A buckle 204 is provided at the connection between the consumable end 200 and the reagent end 100. The buckle 204 is located between the guide slides 205 on both sides. It can be understood that the guide slide 205 on the consumable end 200 is slowly inserted from the bottom of the slide groove 111 on the reagent end 100 until the top of the guide slide 205 abuts against the bottom of the upper limit position 113 of the slide on the reagent end 100. When the guide slide 205 slides upward, the buckle 204 is first squeezed. When the bottom of the buckle 204 gradually comes to the top of the buckle fixing position 112, the bottom of the buckle 204 is locked on the buckle fixing position 112 on the reagent end 110, realizing the vertical limit and playing a fastening role.

[0036] Example 2 like Figures 1-5 As shown, this utility model provides a nucleic acid extraction reagent system, including the nucleic acid extraction reagent strip of Example 1 and the reagent strip holder 300, wherein the nucleic acid extraction reagent strip is movably installed in the reagent strip holder 300.

[0037] The reagent strip holder 300 has a fixing head fixing position 302 at one end of the tray for holding the reagent strip, and raised fixing positions 301 on both sides of the middle of the tray of the reagent strip holder 300.

[0038] A reinforcing rib 109 is provided below the reagent end 100. The reinforcing rib 109 is used to support the reagent strip body and act as a slide rail. The reinforcing rib 109 enables the reagent strip body to slide when installed on the reagent strip holder 300.

[0039] A fixing head 108 is provided at the bottom of one end of the reagent end 100, and protrusions 110 are provided on both sides of the other end. The reagent strip body can be connected to the fixing head fixing position 302 through the fixing head 108; the reagent strip body can be connected to the protrusion fixing position 301 through the protrusions 110.

[0040] The above technical features constitute the preferred embodiment of this utility model, which has strong adaptability and optimal implementation effect. Non-essential technical features can be added or removed according to actual needs to meet the needs of different situations.

[0041] Finally, it should be noted that the above content is only used to illustrate the technical solution of this utility model, and is not intended to limit the scope of protection of this utility model. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of this utility model do not depart from the essence and scope of the technical solution of this utility model.

Claims

1. A nucleic acid extraction reagent strip, comprising a reagent strip body, characterized in that, The reagent strip body is composed of a reagent end at one end and a consumable end at the other end, which are detachably connected. There are grooves on both sides of the connection between the reagent end and the consumable end, and guide rails on both sides of the connection between the consumable end and the reagent end. The guide rails are used to insert into the groove of the reagent end to detachably connect the consumable end and the reagent end.

2. The nucleic acid extraction reagent strip according to claim 1, characterized in that, The connection between the consumable end and the reagent end is equipped with a buckle, which is located between the guide slides on both sides; the bottom of the slide groove of the reagent end is provided with a buckle fixing position, and the top of the slide groove is set as the upper limit position of the slide; when the consumable end and the reagent end are connected, the guide slide is inserted into the slide groove, and the top of the guide slide abuts against the bottom of the upper limit position of the slide, and the bottom of the buckle is locked on the buckle fixing position.

3. The nucleic acid extraction reagent strip according to claim 1, characterized in that, The reagent end has multiple reagent wells, which are used to store various reagents needed for nucleic acid extraction; each reagent well is uniformly sealed with a membrane.

4. The nucleic acid extraction reagent strip according to claim 3, characterized in that, The reagent end is arranged sequentially towards the consumable end as follows: pyrolysis well, idle well one, cleaning well one, cleaning well two, idle well two, magnetic bead preparation / recovery well, and elution well.

5. The nucleic acid extraction reagent strip according to claim 4, characterized in that, The lysis well, idle well one, cleaning well one, cleaning well two, idle well two, magnetic bead preparation / recovery well, and elution well are all reagent wells.

6. The nucleic acid extraction reagent strip according to claim 5, characterized in that, The consumable end is provided with a magnetic rod sleeve hole and multiple tip suction head holes in sequence.

7. A nucleic acid extraction reagent strip according to claim 6, characterized in that, There are two tip nozzle holes, including tip nozzle hole two and tip nozzle hole one.

8. A nucleic acid extraction reagent system, comprising a reagent strip holder, characterized in that, It also includes the nucleic acid extraction reagent strip of any one of claims 1-7 that can be movably mounted on the reagent strip holder; a reinforcing rib that also functions as a slide rail is provided below the reagent end, so that the reagent strip body can slide when mounted on the reagent strip holder.

9. The nucleic acid extraction reagent system according to claim 8, characterized in that, The reagent strip holder has a fixing head at one end of the tray and a fixing head at the bottom of the reagent end. The reagent strip body can be connected to the fixing head by the fixing head.

10. A nucleic acid extraction reagent system according to claim 9, characterized in that, The reagent strip holder has raised fixing positions on both sides of the middle of the tray, and raised positions on both sides of the other end of the reagent strip. The reagent strip body can be connected to the raised fixing positions through the raised positions.

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