A high pressure immunohistochemistry system

Abstract

The utility model relates to a kind of biological reaction devices, especially a kind of high-pressure immunohistochemical system.The utility model provides a kind of high-pressure immunohistochemical system, it includes hot repair reaction device, incubator, turnover mechanism;The hot repair reaction device, incubator and turnover mechanism;The hot repair reaction device and incubator can be housed sample carrier;The turnover mechanism is used to overturn or / and move sample carrier.The utility model is through the cooperation of hot repair reaction device, incubator and turnover mechanism, realizes the accurate positioning of sample carrier in different operating stages.Turnover mechanism can ensure that sample realizes the mode of covering in liquid-adding and incubation link, reduces reagent loss and evaporation.

Description

Technical Field

[0001] This utility model relates to a bioreactor, and more particularly to a high-pressure immunohistochemistry system. Background Technology

[0002] In the field of biotechnology, immunohistochemistry is a commonly used method to study the expression of specific proteins in cell or tissue samples. This technique has wide applications in medical diagnostics, drug development, and basic scientific research.

[0003] Immunohistochemistry is a technique that uses the basic principles of immunology—antigen-antibody reaction, namely the principle of specific binding between antigens and antibodies—to identify antigens (peptides and proteins) in tissue cells by making chromogenic agents (fluorescein, enzymes, metal ions, isotopes) labeled with antibodies show color through chemical reactions. It is used to study the localization, qualitative and relative quantification of these antigens. This technique is called immunohistochemistry or immunocytochemistry.

[0004] In existing immunohistochemistry systems, the sample carrier (such as a glass slide) is typically placed face up during reagent addition and incubation, which has the following drawbacks: 1. Reagent loss and evaporation: Since the sample surface is exposed to the air, reagents are easily evaporated or lost from the slide, causing the sample tissue to dry out directly in the air, which affects the experimental results.

[0005] 2. Reagents react with non-antigen substances: Exposed samples are prone to reacting with substances in the air, leading to an increase in non-specific reactions and affecting the accuracy of the test. Summary of the Invention

[0006] (a) Technical problems to be solved In view of the above-mentioned shortcomings and deficiencies of the prior art, the present invention provides a high-pressure immunohistochemistry system. The flipping mechanism allows the sample carrier to be inverted on the container containing the reagent, preventing reagent loss and evaporation, reducing non-specific reactions and enabling a more efficient incubation process.

[0007] (II) Technical Solution To achieve the above objectives, the main technical solutions adopted by this utility model include: In a first aspect, this utility model provides a high-pressure immunohistochemistry system, which includes a thermal repair reaction device, an incubation device, and a flipping mechanism; Both the thermal repair reaction device and the incubation device can accommodate sample carriers; The flipping mechanism is used to flip and / or move the sample carrier.

[0008] This invention achieves precise positioning of the sample carrier at different operational stages through the cooperation of a thermal repair reaction device, an incubation device, and a flipping mechanism. The flipping mechanism ensures that the sample is covered during liquid addition and incubation, reducing reagent loss and evaporation.

[0009] Optionally, the thermal repair reaction device includes: a reactor body, a sample carrier placement rack, a reaction driving mechanism, and a heating element; In this invention, the reactor body contains a heating element, which provides a stable temperature environment for the sample to undergo dewaxing and thermal repair treatment. The reaction drive mechanism can move the reactor lid, making the loading and unloading of the sample carrier more convenient.

[0010] The reactor body includes a reactor wall and a reactor cover, which form a accommodating cavity for accommodating sample carriers; The reactor cover is connected to a sample carrier placement rack on the side near the accommodating cavity; The reactor cover is driven by a reaction-driven mechanism to move closer to or away from the reactor wall; The heating element is mounted on the reactor body.

[0011] Optionally, the thermal repair reaction apparatus further includes a temperature sensor and a thermal repair control unit, wherein the temperature probe of the temperature sensor is disposed on the reactor body; The thermal repair control unit includes a temperature control module and a drive module. The temperature control module is electrically connected to the temperature sensor and is used to control the heating element based on the temperature reading of the temperature sensor. The drive module is electrically connected to the reaction drive mechanism and is used to drive the movement of the reactor cover.

[0012] In this scheme, the temperature sensor is connected to the thermal repair control unit, and the output of the heating element is controlled through temperature feedback to ensure that the temperature in the reaction device is always within the set range, thus ensuring the accuracy and repeatability of the experiment.

[0013] Optionally, the sample carrier placement rack is provided with a limiting component to restrict the vertical placement of the sample carrier.

[0014] In this design, the limiting components ensure that the sample carrier can be placed vertically during loading, unloading, and flipping, avoiding tilting and displacement, and improving the accuracy of incubation and processing.

[0015] The positioning element keeps the sample carriers upright, preventing them from tilting or shifting during operation and ensuring stable reactions during heating or incubation. Vertical placement of the sample carriers saves space, allowing the system to hold more samples in a smaller volume, thus improving operational efficiency. The vertical and dense arrangement of the samples enables higher pressure to be achieved in a smaller space, improving the effectiveness of thermal remediation within the reactor body. Uniform heating: The vertical arrangement ensures that each sample carrier is evenly exposed to the heat of the heating element, resulting in uniform dewaxing and thermal remediation. In summary, the positioning element design not only ensures that the sample carriers remain upright during operation but also improves the system's space utilization, achieving more efficient and stable immunohistochemical processing.

[0016] Optionally, the flipping mechanism includes a rotating arm, a flipping motor, and a first suction component. The rotating arm is provided with a first suction element for adsorbing sample carriers at its end. The first suction element is used to adsorb and release sample carriers. The output shaft of the flip motor is connected to the rotating shaft of the rotating arm to drive the rotating arm to rotate. In this solution, the rotating arm can pick up or put down the sample carrier by driving the flipping motor, realizing the flipping or moving operation and ensuring the correct positioning of the sample carrier between different devices.

[0017] Optionally, it includes a flip control unit, which is connected to the flip motor via a signal; Optionally, it also includes an automated sample carrier transfer device, which includes a first robotic arm assembly. The first robotic arm assembly includes a robotic arm, a rotating arm mounted on the robotic arm and rotating between the Y and Z directions, and a flip control unit. The flip control unit is connected to the flip motor in the flip mechanism via a signal.

[0018] Optionally, the automatic sample carrier transfer device further includes a third robotic arm assembly and a transfer control unit. The third robotic arm assembly is provided with a second absorbent, which can simultaneously absorb and transfer more than one sample carrier. The transfer control unit controls the rotation of the third robotic arm by connecting to the transfer motor in the third robotic arm assembly via a signal.

[0019] Optionally, the first robotic arm assembly includes a first X-axis movement group, a first Y-axis movement group, and a first Z-axis movement group; the first Y-axis movement group is provided with a rotating arm that rotates between the Y and Z axes.

[0020] Optionally, it also includes a transfer module for accommodating more than one sample carrier.

[0021] Optionally, it also includes a liquid dispensing device, which includes a liquid dispensing body and a reagent assembly and a cleaning assembly disposed on the liquid dispensing body; The reagent assembly includes a pipette and a reagent driving mechanism mounted on the dispensing body; The reagent driving mechanism drives the pipette to move up and down relative to the liquid dispensing body Z, causing the pipette tip to extend downward and retract. The cleaning assembly includes a suction pipe, an air blowing pipe, a liquid outlet pipe, and a cleaning drive mechanism disposed on the liquid adding body; the cleaning drive mechanism drives the cleaning assembly to move up and down relative to the liquid adding body Z. In this design, the pipette moves up and down along the Z-axis under the drive of the reagent driving mechanism, achieving precise reagent dispensing. The aspiration tube, air blowing tube, and dispensing tube are driven by a cleaning driving mechanism to perform sample cleaning operations. The entire process can be controlled by a transfer control unit.

[0022] Optionally, it also includes a liquid addition control unit, which drives the movement of the cleaning component and the reagent component respectively via signal connection to the reagent motor of the reagent driving mechanism and the cleaning motor of the cleaning driving mechanism.

[0023] Optionally, it further includes a second robotic arm assembly, the second robotic arm assembly including a second X-axis movement group and a second Y-axis movement group disposed on the second X-axis movement group; the liquid dispensing device is disposed on the second Y-axis movement group.

[0024] Optionally, it also includes a fixed pipette tip holder, which has several slots for placing pipette tips and several pipette tips that are locked in the slots. The pipette tips are configured to adsorb different reagents.

[0025] This protocol provides a quick procedure for changing multiple reagents, preventing cross-contamination and improving experimental efficiency.

[0026] Optionally, the incubation device includes an incubation rack comprising multiple parallel incubation trays, each tray being connected to an adjacent tray via at least one support structure; each tray can be independently extended. The independent extension of each tray allows for flexible sample handling and ensures effective incubation under different experimental requirements.

[0027] The independent ejection of the incubation tray, in conjunction with the flipping mechanism, allows for the pre-addition of reagents to the incubation tray. The flipping mechanism then flips and moves the sample carrier so that the side carrying the sample is inverted and attached to the incubation tray, facilitating contact between the reagents and the sample for sample or liquid addition. The tray remains attached for incubation.

[0028] Optionally, the incubation rack is further provided with an air blowing plate, which can be independently extended relative to the incubation plate; the air blowing plate is provided with an air blowing port. The independent extension of the air blowing plate provides an air blowing function, ensuring uniform reagent adhesion and reducing interference from residual liquid on the detection.

[0029] Optionally, the incubation rack is further provided with a washing tray, the inner side wall of which is provided with a water outlet. The water outlet on the inner side of the washing tray can ensure thorough cleaning of the sample carrier, reduce interference from impurities, and provide a clean environment for subsequent reactions.

[0030] Optionally, it also includes a third robotic arm assembly, which can simultaneously adsorb and transfer more than one sample carrier; it can process multiple sample carriers at the same time, shorten the operation time, and significantly improve the system efficiency.

[0031] Optionally, it also includes a transfer module for accommodating more than one sample carrier.

[0032] Secondly, this utility model provides a high-pressure immunohistochemistry method, which includes the following steps: S1 Dewaxing and thermal retrieval of sample carriers containing samples; S2 involves more than one incubation, the incubation method including: flipping the sample carrier so that the side containing the sample covers the container with reagents so that the reagents adhere to the sample, and then incubating after adding the liquid; S3 colorimetric reaction: After incubation, the sample carrier is added to the colorimetric reagent to carry out the colorimetric reaction.

[0033] In this scheme, the flipping operation moves the sample away from the surface or other non-specific substances to participate in the incubation and other reaction steps, reducing noise and improving the specificity of the reaction.

[0034] Optionally, it further includes the following steps: In step S2, the sample carrier is cleaned after incubation. The cleaning method includes: flipping the sample carrier to expose the side carrying the sample, and cleaning with water.

[0035] Optionally, dewaxing and thermal repair are carried out in a thermal repair reaction apparatus; The sample carrier is removed from the thermal repair reaction device by a flipping mechanism, and the sample carrier is flipped so that the side containing the sample is upside down. The sample carrier is moved to the incubation device by a flipping mechanism to carry out steps including adding liquid and incubation.

[0036] Optionally, the incubation may include primary antibody incubation; the sample vector may be incubated with primary antibody reagent. Optionally, the incubation process may include secondary antibody incubation; the sample vector may be incubated with secondary antibody reagent. Optionally, the primary antibody reagent may be an amplifying agent or an enhancing agent; Optionally, the colorimetric reagent is a DAB reagent.

[0037] Optionally, it also includes the following steps: In step S2, the sample carrier is cleaned after incubation. The cleaning method includes: flipping the sample carrier to expose the side carrying the sample, and cleaning with water.

[0038] Optionally, the dewaxing and thermal repair are carried out in a thermal repair reaction apparatus; The sample carrier is removed from the thermal repair reaction device by a flipping mechanism, and the sample carrier is flipped so that the side containing the sample is upside down. The sample carrier is moved to the incubation device by a flipping mechanism to carry out steps including adding liquid and incubation.

[0039] In this scheme, the flipping mechanism can accurately move the sample carrier between the thermal repair reaction device and the incubation device, realizing continuous operation of incubation and color development reaction, and improving the degree of automation of the experiment.

[0040] (III) Beneficial Effects The beneficial effects of this utility model are: This invention provides a high-pressure immunohistochemistry system suitable for high-pressure immunohistochemistry methods. By improving the moisture retention and coverage of reagents, it significantly enhances the efficiency and quality of immunohistochemistry experiments and solves the problems of reagent evaporation and sample drying in traditional techniques. It is an innovative design with significant advantages.

[0041] Among its features, the special inverting mechanism prevents reagent loss and evaporation: the sample carrier is inverted to cover the container containing the reagents, avoiding direct exposure of the reagents to the air, reducing evaporation, and ensuring that the reagents are in full contact with the sample surface during incubation, thus improving experimental stability; it also reduces non-specific reactions: the inverted design ensures full contact between the sample and the reagents inside the container while isolating them from the air, effectively reducing the chance of non-antigen substances reacting with the reagents, thereby reducing noise and improving sensitivity; and it enables a more efficient incubation process: the reagents can more evenly and fully cover the sample, ensuring the effectiveness and consistency of incubation, and enhancing the sensitivity and accuracy of immunohistochemical reactions.

[0042] Furthermore, this invention utilizes a thermal repair reaction device for dewaxing and thermal repair, which has a sealed cavity that allows for simultaneous heating and pressurization. Attached Figure Description

[0043] Figure 1 This is a schematic diagram of the high-pressure immunohistochemistry system of this invention; Figure 2 This is a schematic diagram of the structure of the first robotic arm assembly and the flipping mechanism of this utility model; Figure 3This is a schematic diagram of the thermal repair reaction device of this utility model; Figure 4 This is a more detailed structural schematic diagram of the thermal repair reaction device of this utility model; Figure 5 This is a schematic diagram of the structure of the first sample carrier placement area of ​​this utility model; Figure 6 This is a schematic diagram of the liquid addition device of this utility model; Figure 7 This is a schematic diagram of the structure of the incubation device of this utility model; Figure 8 This is a schematic diagram of the structure of the first robotic arm assembly, the second robotic arm assembly, and the third robotic arm assembly of this utility model; Figure 9 This is a schematic diagram of the reagent storage tank of this utility model; Figure 10 This is a schematic diagram of the push-pull component of this utility model; Figure 11 This is an enlarged schematic diagram of the air blowing plate of this utility model; Figure 12 This is an enlarged schematic diagram of the structure of the cleaning disc of this utility model.

[0044] The reference numerals used in the above figures are explained as follows: 1. Thermal repair reaction device; 11. Reactor body; 111. Reactor wall; 112. First outlet; 113. Inlet; 114. Exhaust port; 115. Temperature probe; 116. Heating element; 117. Receptacle; 118. Top cover; 119. Bottom cover; 12. First sample carrier placement area; 13. Tank; 14. Lifting mechanism; 15. Stepper motor; 16. Lead screw; 17. Bracket; 18. Support rod; 19. Connecting rod; 2. Incubation apparatus; 21. First incubation rack; 22. Second incubation rack; 23. Incubation tray; 24. Air blowing tray; 25. Air blowing port; 26. Push-pull component; 261. Push-pull rod; 262. Fastening block; 263. Fastening hole; 27. Cleaning tray; 28. Second water outlet; 29. ​​Pipette tip holder; 3. First robotic arm assembly; 31. Tilting mechanism; 311. Rotating arm; 312. Tilting motor; 313. First suction component; 32. First X-axis moving group; 33. First Y-axis moving group; 34. First Z-axis moving group; 4. Workbench; 41. Crossbar; 5. Reagent storage tank; 6. Liquid addition device; 61. Liquid addition body; 621. Pipette; 631. Suction tube; 632. Air blowing tube; 633. Liquid outlet tube; 7. Second robotic arm assembly; 71. Second robotic arm; 8. Third robotic arm assembly; 81. Third Y-axis movement group; 82. Third Z-axis movement group; 83 Second suction component; 9. Transfer module; 10. Sample carrier. Detailed Implementation

[0045] To better explain and facilitate understanding of this invention, exemplary embodiments of the invention will be described in more detail below. While exemplary embodiments of the invention are shown below, it should be understood that the invention can be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this invention will be clearer and more thorough in its understanding and will fully convey the scope of the invention to those skilled in the art.

[0046] In this document, the term "embodiment" means that a specific feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The term "embodiment" appearing in various places throughout the specification does not necessarily refer to the same embodiment, nor does it specifically limit its independence or connection with other embodiments. In principle, in this application, as long as there are no technical contradictions or conflicts, the technical features mentioned in each embodiment can be combined in any way to form corresponding implementable technical solutions.

[0047] Unless otherwise defined, the technical terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains; the use of related terms herein is merely for the purpose of describing particular embodiments and is not intended to limit this application.

[0048] In the description of this application, the term "and / or" is used to describe the logical relationship between objects, indicating that three relationships can exist. For example, A and / or B means: A exists, B exists, and A and B exist simultaneously. Additionally, the character " / " in this document generally indicates that the preceding and following objects have an "or" logical relationship.

[0049] In this application, terms such as “first” and “second” are used only to distinguish one entity or operation from another, and do not necessarily require or imply any actual quantity, hierarchy or order relationship between these entities or operations.

[0050] Unless otherwise specified, the use of terms such as “comprising,” “including,” “having,” or other similar expressions in this application is intended to cover non-exclusive inclusion, which does not exclude the presence of additional elements in a process, method, or product that includes the stated elements, such that a process, method, or product that includes a list of elements may include not only those defined elements but also other elements not expressly listed, or elements inherent to such a process, method, or product.

[0051] As understood in the Examination Guidelines, in this application, expressions such as "greater than," "less than," and "exceeding" are understood to exclude the stated number; expressions such as "above," "below," and "within" are understood to include the stated number. Furthermore, in the description of the embodiments in this application, "multiple" means two or more (including two), and similar expressions related to "multiple" are also understood in this way, such as "multiple groups" and "multiple times," unless otherwise explicitly specified.

[0052] In the description of the embodiments of this application, the space-related expressions used, such as "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "vertical," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential," indicate the orientation or positional relationship based on the orientation or positional relationship shown in the specific embodiments or drawings. They are only for the purpose of describing the specific embodiments of this application or for the reader's understanding, and do not indicate or imply that the device or component referred to must have a specific position, a specific orientation, or be constructed or operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this application.

[0053] Unless otherwise expressly specified or limited, the terms "installation," "connection," "linking," "fixing," and "setting," as used in the description of the embodiments of this application, should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral setting; it can be a mechanical connection, an electrical connection, or a communication connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be the internal connection of two components or the interaction between two components. For those skilled in the art to which this application pertains, the specific meaning of the above terms in the embodiments of this application can be understood according to the specific circumstances.

[0054] It should be noted that the sample carrier described in this utility model includes, but is not limited to, glass slides, and may include any container or carrier having a side on which a sample can be placed.

[0055] The X, Y, and Z directions referred to in this utility model do not only refer to the directions of the arrows on the X, Y, and Z axes in the accompanying drawings, but can be the opposite directions indicated by the arrows on the X, Y, and Z axes.

[0056] Example 1 like Figure 1 As shown: This embodiment provides a high-pressure immunohistochemistry system, which includes a heat repair reaction device 1, an incubation device 2, and a flipping mechanism 31; both the heat repair reaction device 1 and the incubation device can accommodate the sample carrier 10; the flipping mechanism 31 is used to flip or / and move the sample carrier 10.

[0057] In this embodiment, the flipping mechanism 31 is used to flip and / or move the sample carrier 10, and through the cooperation of the thermal repair reaction device 1, the incubation device, and the flipping mechanism 31, the sample carrier 10 is precisely positioned at different operational stages. The flipping mechanism 31 ensures that the sample is inverted and covered during the liquid addition and incubation stages, reducing reagent loss and evaporation.

[0058] In this embodiment, the flipping mechanism 31 can flip the sample carrier 10 so that the sample carrier 10, such as a glass slide, has the side containing tissue or other samples facing down, and then moves it to the incubation device for incubation. The incubation device 2 has a first sample carrier placement area, where the reagent to be reacted is pre-added. After the flipping mechanism 31 flips and inverts the sample carrier 10, the side of the sample carrier 10 containing tissue or other samples facing down covers any area containing the reaction reagent, completing the addition of the reagent while continuing the incubation reaction in a covered manner.

[0059] In this embodiment, the flipping mechanism 31 can be any other structure that can flip the sample carrier 10 upside down so that the side of the sample carrier 10 with the tissue sample facing down.

[0060] In some other specific embodiments, such as Figure 2 As shown, the flipping mechanism 31 includes a rotating arm 311, a flipping motor 312, and a first suction member 313. The end of the rotating arm 311 is provided with a first suction member 313 for adsorbing the sample carrier 10. The first suction member 313 is used to adsorb and release the sample carrier 10. The output shaft of the flipping motor 312 is connected to the rotating shaft of the rotating arm 311, driving the rotating arm 311 to rotate.

[0061] In some other embodiments, the flipping mechanism 31 is driven to move by a robotic arm. The robotic arm and the flipping mechanism 31 constitute the first robotic arm assembly 3 in the automatic slide transfer module. The first robotic arm assembly 3 further includes a first X-axis moving group 32, a first Y-axis moving group 33, and a first Z-axis moving group 34. The first Y-axis moving group 33 is provided with a rotating arm 311 that can rotate between the Y and Z axes. The output shaft of the flipping motor 312 is rotatably connected to the rotating arm 311. In some other embodiments, the high-pressure immunohistochemistry system also includes a workbench 4, and a thermal repair reaction device 1, an incubation device 2, and a robotic arm are disposed on the workbench 4. The flipping mechanism 31 is connected to the workbench 4 via the robotic arm.

[0062] In some other embodiments, the first robotic arm assembly 3 also includes a flip control unit. The flip control unit is connected to the flip motor 312 in the flip mechanism 31 via a signal connection.

[0063] In some other specific embodiments, the first suction member 313 is one or more suction cups disposed at the end of the rotating disk. A vacuum interface is provided inside the suction cup, which is connected to a vacuum pump pipeline. The vacuum pump controls a switch via a first solenoid valve to enable the suction cups to pick up and place the glass slides. The first solenoid valve is controlled by a suction control unit to control its suction and placement.

[0064] Example 2 like Figure 3 , 4 As shown in Figure 5, this embodiment provides a high-pressure immunohistochemistry system, which, compared to Embodiment 1, includes a thermal repair reaction device 1 comprising: The reactor includes a reactor body 11, a heating element 116, a temperature probe 115, a sample carrier placement area 12, a lifting mechanism 14, and a thermal repair control unit. The reactor body 11 must meet the dewaxing and repair requirements of immunohistochemistry in the biomedical field, such as heating uniformity, durability and safety, ease of cleaning, and compatibility with commonly used immunohistochemical reagents and solutions. It can be made of materials such as stainless steel, aluminum alloy, or ceramic, and its shape is not particularly limited, including but not limited to cylindrical, square, and rectangular shapes, but a cylindrical stainless steel reactor is preferred. The reactor body 11 has a reactor wall 111, a top cover 118, and a bottom cover 119. The reactor wall 111 and the bottom cover 119 enclose a receiving cavity 117, which is used to hold the test sample. During the dewaxing and repair process, the test sample is the slide assembly to be dewaxed and repaired. The heating element 116 is, for example, an electric heating wire, an electric heating tube, an electric heating plate, an electromagnetic coil, an electric heating film, a graphite heating element, etc., and is disposed separately in the reactor wall 111 or the accommodating cavity 117, or simultaneously in the reactor wall 111 and the accommodating cavity 117. It is preferred to be disposed separately in the reactor wall 111 to avoid occupying space and unnecessary experimental risks in the accommodating cavity 117. The temperature probe 115 is disposed on the top cover 118. In this embodiment, the first sample carrier placement area 12 is a box or basket-shaped slide holder made of a material that can withstand high temperatures above 60°C and has a slide restraint structure that can place slides horizontally or vertically. The lifting mechanism 14 includes a stepper motor 15, a lead screw 16, a bracket 17, a support rod 18, and some fixing screws, nuts, and other accessories. The stepper motor 15 is fixedly mounted on the bracket 17 and connected to the top cover 118 located directly below the stepper motor 15 via the lead screw 16. The top cover 118 is connected to the first sample carrier placement area 12 via a connecting rod 19. The maximum distance between the top cover 118 and the first sample carrier placement area 12 is less than or equal to the depth of the accommodating cavity 117. Thus, when the top cover 118 descends with the lead screw 16, the slide placement area also gradually descends into the accommodating cavity 117, ensuring that the top cover 118 can be completely closed with the upper part of the reactor body 11. The support rod 18 passes through the edge of the top cover 118, supports the bracket 17, and is used for the lifting and lowering movement of the top cover 118 along the support rod 18. The thermal repair control unit includes a temperature control module and a drive module. The temperature control module is electrically connected to the temperature probe 115 and is used to control the heating element 116 based on the temperature reading of the temperature probe 115. The drive module is electrically connected to the lifting mechanism 14 and is used to drive the lifting mechanism 14 to move up or down.

[0065] These embodiments automate the thermal restoration process, automatically exposing or sealing the slide placement area, effectively reducing the complexity and potential errors of manual operations. This shortens the total time required for thermal restoration, which is significant for accelerating experimental procedures and improving laboratory efficiency.

[0066] Example 3 This embodiment provides a high-pressure immunohistochemistry system, which, compared to Embodiment 1, is as follows: Figure 6 As shown, it also includes a liquid adding device 6. The liquid dispensing device 6 includes a liquid dispensing body 61 and a reagent assembly and a cleaning assembly disposed on the liquid dispensing body 61; the reagent assembly includes a pipette 621 and a reagent driving mechanism disposed on the liquid dispensing body 61. The reagent driving mechanism drives the pipette 621 to move up and down relative to the liquid addition body 61, so that the tip of the pipette 621 extends downward and retracts. In some other specific embodiments, the reagent driving mechanism includes a reagent motor, a rotating shaft, gears, and a drive belt. The output shaft of the reagent motor is connected to the rotating shaft. Rotating shafts are respectively provided at the upper and lower ends of the liquid addition body 61. Gears are fixedly provided on the rotating shafts, and drive belts are provided on the gears. Part of the drive belts are fixedly connected to the pipette 621. In some other specific embodiments, the pipette 621 includes a body, a pipette tip, a suction button, a tip ejection button, and an electric telescopic mechanism. The pipette tip is located below the body, and the pipette tip connector, piston mechanism, and ejection mechanism control element are also included. The telescopic end of the electric telescopic mechanism faces downward and is directly opposite the top of the liquid suction button. The electric telescopic mechanism is connected to the power supply through a controller, and the controller controls the extension and retraction of the electric telescopic mechanism and sends control signals to the controller.

[0067] In some other specific embodiments, such as Figure 7 As shown, it also includes a fixedly mounted pipette tip holder 29; the pipette tip holder 29 is provided with several slots for placing pipette tips and several pipette tips that are locked in the slots, and the several pipette tips are configured to adsorb different reagents. The pipette tip holder 29 can be fixedly mounted on the worktable 4.

[0068] In some other specific embodiments, like Figure 6 As shown, the cleaning assembly includes a suction pipe 631, an air blowing pipe 632, a liquid outlet pipe 633, and a cleaning drive mechanism disposed on the liquid adding body 61; the cleaning drive mechanism drives the cleaning assembly to move up and down in the Z direction relative to the liquid adding body 61. In some other specific embodiments, the cleaning assembly includes a suction pipe 631, an air blowing pipe 632, a liquid outlet pipe 633, a water tank, a waste liquid tank, and a reagent driving mechanism disposed on the liquid adding body 61. The water tank and waste liquid tank are detachably mounted on the liquid filling body 61. The other end of the outlet pipe 633 is connected to the outlet of the water tank. A first valve is installed on the outlet pipe 633, and the outlet of the outlet pipe 633 is set downwards. The other end of the suction pipe 631 is connected to the inlet of the waste liquid tank. A second valve is installed on the suction pipe 631, and the outlet of the suction pipe 631 is set downwards. The outlet of the air blowing pipe 632 is set downwards. The outlets of the outlet pipe 633, the suction pipe 631, and the air blowing pipe 632 are not on the same horizontal plane. Preferably, the outlet of the suction pipe 631 is set at the bottom and the outlet of the outlet pipe 633 is set at the top. In some other embodiments, it also includes a cleaning control unit that controls the opening and closing of the first and second valves via signals.

[0069] In some other specific embodiments, such as Figure 1 , 7 As shown in Figure 8, it also includes a second robotic arm assembly 7, which includes a second X-axis moving group and a second Y-axis moving group disposed on the second X-axis moving group; the liquid dispensing device 6 is disposed on the second Y-axis moving group. The second robotic arm assembly 7 drives the liquid dispensing device 6 to move in three-dimensional directions, so that the liquid dispensing device 6 moves to the required position to add reagent.

[0070] In some other specific embodiments, such as Figure 1 and 7 The worktable shown is a three-dimensional worktable 4. The second X-axis moving group includes a crossbar 41 set on the three-dimensional worktable 4 and a second robotic arm 71 driven by a second X-axis motor to move on the crossbar 41. The second Y-axis moving assembly includes a second Y-axis slider mounted on the second robotic arm 71 and sliding along the second robotic arm 71, and a second Y-axis motor that drives the second Y-axis slider; the liquid adding device 6 is connected to the second Y-axis slider.

[0071] In some other specific embodiments, it also includes a liquid dispensing control unit, which drives the movement of the cleaning component and the reagent component respectively by means of a reagent motor of the reagent driving mechanism and a cleaning motor of the cleaning driving mechanism; and controls the movement of the liquid dispensing component in the X and Y directions by means of a second X-axis motor and a second Y-axis motor.

[0072] In some other specific embodiments, such as Figure 9 As shown, the workbench 4 is also equipped with several reagent storage slots 5, which are used to hold fixed reagent bottles. The liquid dispensing control unit is connected to the electric telescopic mechanism via a signal. The liquid dispensing control unit controls the liquid dispensing device 6 to draw specific reagents at specific positions in the reagent storage slots 5 by coordinating the control of the second X-axis motor, the second Y-axis motor 73, and the electric telescopic mechanism.

[0073] The high-pressure immunohistochemistry system provided in this embodiment integrates a thermal retrieval reaction device 1, an incubation device 2, and a robotic arm device on a single workbench 4, which can automatically complete the sample processing, incubation, and cleaning process, significantly improving experimental efficiency and sample processing speed.

[0074] The system uses a robotic arm to automatically transfer sample carriers and dispense reagents, reducing human intervention during the experiment, thereby reducing potential operational errors and improving the accuracy and repeatability of experimental results.

[0075] Automation allows users to complete the entire experimental process automatically by simply setting relevant parameters, reducing operational complexity.

[0076] This embodiment can not only effectively improve the level of automation and efficiency of experiments, but also ensure the accuracy and reliability of experimental results, making it suitable for high-throughput biomedical research and clinical diagnosis.

[0077] In other specific embodiments, the workbench 4, the thermal repair reaction device 1, the incubation device 2, the reagent assembly, and the cleaning assembly may be structures with the same function as those described in the prior art.

[0078] Example 4 This embodiment provides a high-pressure immunohistochemistry system, which, compared to embodiments 1-3, further includes: Figure 7 As shown, the incubation device includes a first incubation rack 21 and a second incubation rack 22. Both the first incubation rack 21 and the second incubation rack 22 include a plurality of parallel incubation trays 23. Each incubation tray 23 is connected to an adjacent layer through at least one support structure. Each incubation tray 23 is provided with a plurality of slots for placing sample carriers 10, forming a first sample carrier placement area. Each incubation tray 23 can be independently extended.

[0079] The first incubation rack 21 or the second incubation rack 22 is further provided with an air blowing plate 24 parallel to the incubation tray 23, such as Figure 10 and 11 As shown, the air blowing plate 24 can be independently extended relative to the incubation plate 23; the air blowing plate 24 is provided with an air blowing port 25, which is configured for directional airflow, preferably vertically upward; the air blowing plate 24 is connected to the incubation rack through two guide rails, and the guide rails are controlled by a motor to push the air blowing plate 24 to move along the guide rails, and the motor is controlled by a controller.

[0080] The air inlet 25 is connected to a compressed air source or air pump via an air pipe 632. The push-pull member 26 is connected to the incubation tray 23 and is used to push out the incubation tray 23; The controller is connected to a compressed air source or air pump via a signal to control the opening, closing, and operating intensity of the compressed air source or air pump. The controller is connected to the transfer motor via a signal, and controls the motor to drive the push-pull component 26 forward.

[0081] like Figure 12 As shown, a cleaning tray 27 is also provided at the lower end of the first incubation rack 21 or the second incubation rack 22, and a second water outlet 28 is provided on the inner side wall of the cleaning tray 27.

[0082] In some other specific embodiments, the push-pull member 26 is disposed between the first incubation rack 21 and the second incubation rack 22, and each incubation tray 23 is provided with a fastening hole 263; like Figure 9 As shown, the push-pull component 26 includes a main body of the push-pull component 26, a first slider and a second lifting structure disposed on the main body of the push-pull component 26. The first slider is connected to push-pull rods 261 on its left and right sides respectively. The push-pull rods 261 are provided with fastening blocks 262 that cooperate with the fastening holes 263. The fastening blocks 262 are L-shaped structures extending out of the outside of the push-pull rods 261, and one end of the L-shape is set downward. Optionally, the first slider includes a left slider and a right slider, and the left slider and the right slider are respectively connected to a push-pull rod 261.

[0083] The main body of the push-pull component can selectively drive the left and right sliders to move up and down in the Z direction through the second lifting structure, thereby selectively moving the push-pull rod 261 to engage with any one of the incubation trays 23. The main body of the push-pull component moves in the Y direction through the Y-direction moving module, so that the push-pull rod 261 drives the incubation tray 23 to be pushed out.

[0084] The controller connects to the transfer motor via a signal to control the lifting and lowering of the second lifting mechanism 14, and connects to the Y-direction movement module via a signal to control the Y-direction movement of the main body of the push-pull component 26.

[0085] The second lifting mechanism includes a second stepper motor, a second drive screw, and a guide rail. The shaft of the second stepper motor is directly connected to the second drive screw. The second drive screw converts the rotational motion of the second stepper motor into linear motion. The second lead screw passes through a fixed nut, which is fixed to a second slider but allows the second lead screw to rotate freely. When the second stepper motor rotates, the second lead screw does not rotate; instead, it drives the nut and the attached second slider to move along the axial direction of the second lead screw.

[0086] To ensure stable and precise movement of the second slider along the Z-direction, the second lifting mechanism is equipped with two parallel guide rails. The second slider is fitted with ball bearings or second sliders that mate with the guide rails, providing smooth, low-friction movement around the guide rails.

[0087] The Y-axis movement module includes a servo motor, gears, and a guide rail. The Y-axis movement module is powered by a servo motor equipped with a high-precision encoder to monitor and control position and speed, ensuring the accuracy of movement.

[0088] The servo motor transmits power to a rack that meshes with it via a gear connected to its shaft. The rack is fixed to the worktable 4 and extends along the Y-axis in a preset direction of movement. The rotation of the gear causes the main body of the push-pull member 26 connected to the rack to move along the Y-axis.

[0089] The guide rail is used to maintain the linear and stable movement of the main body of the push-pull component 26. The main body of the push-pull component 26 interacts with the guide rail through ball bearings or sliding bearings.

[0090] The main body of the push-pull component 26 is connected to the first slider connecting arm through one or more transverse connecting arms, allowing the linear movement of the second lifting structure in the Z direction to work in conjunction with the linear movement of the Y direction moving module to precisely control the position of the incubation tray 23.

[0091] Example 5 like Figure 1 and 8 As shown, this embodiment provides a high-pressure immunohistochemistry system, which, compared to embodiments 1-4, further includes a third robotic arm assembly 8 and a transfer module 9 in its automatic transfer module. The third robotic arm assembly 8 includes a third Y-axis moving group 81 and a third Z-axis moving group 82 disposed on the third Y-axis moving group; it can simultaneously adsorb and transfer more than one sample carrier 10.

[0092] In some specific embodiments, the first suction member 313 and the second suction member 83 are suction cup structures. The second suction member 83 is disposed on the third Z-axis moving group 82 and includes one or more suction cups, which are arranged downwards. One or more suction cups can cooperate with one or more sample carriers 10 placed on the transfer module 9 for simultaneous adsorption. If the sample carrier 10 is a glass slide, the glass slide is placed vertically in the thermal repair reaction device 1. The controller is connected to the cleaning drive mechanism via a signal to drive the adsorption and release of the suction cups.

[0093] The first suction unit 313 and the second suction unit 83 employ a suction cup structure. These suction cups are designed to generate sufficient negative pressure to stably adsorb vertically placed glass slide sample carriers 10. The suction cups are connected to a vacuum pump via flexible tubing. The vacuum pump is switched on and off by a controller signal to ensure that the suction cups can adsorb or release the glass slides at the appropriate time.

[0094] The controller is connected to the electric telescopic mechanism, the flip control unit, the transfer control unit, the thermal repair control unit, and the suction control unit via signals. During operation, the controller controls the first suction component of the first robotic arm assembly to suction the vertically placed sample carrier 10 from the thermal repair reaction device 1. In particular, it suctions the sample carrier 10 on the side that does not carry the sample. The first robotic arm assembly flips the sample carrier 10 to a horizontal position. Optionally, the sample carrier 10 is inverted and covered on the transfer module 9 with the side carrying the sample. The above steps are repeated until the transfer module 9 has a set number of sample carriers 10. The second suction unit of the third robotic arm assembly is activated. The suction cup of the second suction unit is configured to mate with the positions of multiple sample carriers 10 on the transfer module 9, allowing simultaneous adsorption of multiple sample carriers 10. The third robotic arm assembly transfers the multiple sample carriers to the incubation tray of the incubation device. The third robotic arm assembly does not have a Y-axis movement module; the incubation tray in the incubation device will extend outwards in the Y-axis direction. The third robotic arm assembly is activated, causing the pipette in the liquid dispensing device to move to the pipette storage rack. After extending the pipette tip with the corresponding reagent onto the pipette storage rack, the pipette is retracted. The third robotic arm assembly moves to the reagent storage tank, extends the pipette tip downwards, draws the preset amount from the controller, and moves it to the incubation device, pre-adding reagent to the incubation tray. The third robotic arm assembly inverts the sample carrier to cover it in the reagent, simultaneously incubating it. After incubation, the third robotic arm transfers multiple sample carriers to another incubation tray, preferably to the tray of another incubation rack. Optionally, they can be transferred to a cleaning tray, allowing the sample carriers to adhere to the bottom of the cleaning tray. The water outlet of the cleaning tray is located on the inner wall, with the water outlet in the horizontal Y-axis direction, adapted to the position of each slide. After cleaning, the third robotic arm transfers the sample carriers directly above the air outlet of the air blowing plate. Simultaneously, the motor of the air blowing plate activates the air pump, and the air blowing plate moves back and forth along the guide rail in the Y-axis direction to achieve air blowing. After air blowing, the samples are transferred to a clean incubation tray. Simultaneously, the third robotic arm assembly moves the incubation tray, which has already been incubated, through a cleaning assembly. The cleaning assembly is activated, causing water to flow from the outlet tube onto the incubation tray. Preferably, the incubation tray has multiple grooves forming incubation zones for the sample carriers. Controlling the movement of the cleaning assembly allows the outlet tube to move and spray along the extension direction of the grooves while discharging water. After cleaning, the cleaning assembly moves downwards, allowing the suction tube to contact the incubation tray for suction. Optionally, a suction tube is installed inside the cleaning tray, and a suction pump is controlled by a controller to draw water. After suction, the cleaning assembly retracts upwards, and simultaneously, air is blown out through the air blowing tube to remove water droplets from the incubation tray. After cleaning and air blowing, the tray is clean and ready for the next step of reagent addition and incubation.

[0095] This embodiment can be applied to the full automation of any high-pressure immunohistochemistry method. It can be adapted to all high-pressure immunohistochemistry methods by setting the corresponding parameters of the controller.

[0096] Transfer drive mechanism: Responsible for controlling the suction cup's adsorption and release on the third robotic arm assembly 8. It includes a small electric motor that rotates via gears and linkages to push the suction cup toward or away from the glass slide surface. The motor's movement is fed back to the controller via an encoder to ensure precise control of the suction cup's position.

[0097] The aforementioned controller is equipped with relevant control units (including but not limited to the electric telescopic mechanism, tilt control unit, transfer control unit, thermal repair control unit, aspiration control unit, and thermal repair control unit), which transmit commands to relevant drive mechanisms, including but not limited to the transfer drive mechanism, reagent drive mechanism, cleaning drive mechanism, and transfer motor, via electrical signals to control the precise movements of the pipette 621, cleaning assembly, and suction cup. Upon receiving the signal, the relevant drive mechanism activates the corresponding motor or solenoid valve through its internal circuitry to execute the required action.

[0098] Control system integration: The control system integrates sensor data such as position sensors and pressure sensors to monitor the status of the robotic arm in real time and adjust operating parameters to ensure the accuracy and repeatability of the operation.

[0099] Example 6 This embodiment also provides a method for high-pressure immunohistochemistry liquid addition and incubation reaction, which includes the following steps: inverting the sample carrier 10 containing the sample into a container pre-added with reagents to realize the liquid addition and incubation reaction.

[0100] In the prior art, during the addition of reagents and incubation, the sample carrier 10, such as a glass slide, is set with the side containing tissue or other samples facing upwards, and at most, it is simply treated with a cover film.

[0101] There is a risk that reagents may evaporate or be lost directly from the sample carrier, such as a glass slide. After the reagents are lost, the tissues will be exposed to the air and will dry out, which may lead to experimental failure.

[0102] This embodiment uses a covered, inverted covering method, which can avoid reagent loss and reduce evaporation. At the same time, it reduces the reaction between non-antigen substances and reagents during incubation, reduces non-specific reactions, lowers noise, and improves sensitivity.

[0103] Example 7 This embodiment provides a high-pressure immunohistochemistry method, which includes the following steps: S1 Dewaxing and thermal retrieval of sample carriers containing samples; S2 primary antibody incubation; primary antibody reagent is added to the sample vector for primary antibody incubation; S3 secondary antibody incubation; the sample vector is incubated with secondary antibody reagent; S4 colorimetric reaction; Methods for adding primary antibody reagents or secondary antibody reagents include: Invert the sample carrier so that the loaded side covers the container containing the primary antibody and / or secondary antibody reagents.

[0104] The flipping operation moves the sample away from the surface or other non-specific substances to participate in the incubation and other reaction steps, reducing noise and improving the specificity of the reaction.

[0105] More specifically: dewaxing and thermal repair of the sample carrier (10) containing the sample in S1; S5 colorimetric reaction; DAB reagent was added to the sample carrier (10) for DAB incubation; The secondary antibody reagent can be, but is not limited to, an amplifying agent or an enhancing agent; In the colorimetric reaction, the reagent loaded can be, but is not limited to, DAB reagent; Optionally, it also includes the following steps: After any of steps S1-S4, the method of adding other reagents to the sample carrier for reaction includes: inverting the sample carrier so that the side containing the sample covers the container containing other reagents for reaction. or / and It also includes the following steps: The sample carrier is cleaned by means of: flipping the sample carrier to expose the side containing the sample and rinsing it with water.

[0106] Optionally, the dewaxing and thermal repair are carried out in a thermal repair reaction apparatus; The sample carrier is removed from the thermal repair reaction device by a flipping mechanism, and the sample carrier is flipped so that the side containing the sample is upside down. The sample carrier is moved to the incubation device by a flipping mechanism to carry out the liquid addition and incubation steps, including primary antibody incubation and secondary antibody incubation.

[0107] The flipping mechanism can accurately move the sample carrier between the thermal repair reaction device and the incubation device, enabling continuous operation of incubation and colorimetric reactions and improving the degree of automation of the experiment.

[0108] This embodiment provides a high-pressure immunohistochemistry method, which includes the following steps: S1 The sample carrier 10 containing the sample is dewaxed and thermally repaired in the thermal repair reaction device 1; S2 uses the first suction cup in the first robotic arm assembly 3 to pick up the sample carrier 10 from the thermal repair reaction device 1 and place it on the transfer module 9; S3 uses the liquid addition device 6 in the second robotic arm assembly 7 to pre-add the blocking agent to the incubation device; the second suction cup of the third robotic arm assembly 8 adsorbs and transfers the sample carrier 10 on the transfer module 9 to the incubation device, covering it with the blocking agent in an upside-down manner for the first incubation, and then washes the sample carrier 10 for the first time. S4 uses the liquid addition device 6 in the second robotic arm assembly 7 to pre-add the primary antibody reagent to a clean position of the incubation device; the second suction cup of the third robotic arm assembly 8 adsorbs and transfers the sample carrier 10 after the first cleaning, and covers it on the primary antibody reagent in an upside-down manner for a second incubation, and then cleans the sample carrier 10 for the second time. S6 uses the liquid addition device 6 in the second robotic arm assembly 7 to pre-add amplifying agent to a clean position in the incubation device; the second suction cup of the third robotic arm assembly 8 adsorbs and transfers the sample carrier 10 after the first cleaning, and covers it on the amplifying agent in an upside-down manner for the third incubation; the sample carrier 10 is then cleaned for the third time. S7 uses the liquid addition device 6 in the second robotic arm assembly 7 to pre-add secondary antibody reagent to a clean position of the incubation device, and uses the second suction cup of the third robotic arm assembly 8 to adsorb and transfer the sample carrier 10 after the first cleaning, and covers it on the secondary antibody reagent in an upside-down manner for the fourth incubation; then the sample carrier 10 is cleaned for the fourth time. S8 uses the liquid addition device 6 in the second robotic arm assembly 7 to pre-add substrate to a clean position in the incubation device; the second suction cup of the third robotic arm assembly 8 adsorbs and transfers the sample carrier 10 after the first cleaning, and covers it on the substrate in an upside-down manner for the fifth incubation.

[0109] Example 8 The reactor body 11 of the high-pressure dewaxing and heat repair apparatus for immunohistochemistry is further equipped with a base at its lower part, and a protective sleeve is provided around its perimeter. In addition, a transfer module 9 is further provided for placing the slides after high-pressure dewaxing and heat repair.

[0110] The operation process of this novel high-pressure dewaxing and thermal repair device for immunohistochemistry is as follows: 1. Start-up and preheating of the device: The user starts the device via the touchscreen interface and selects a preset dewaxing and heat repair program. The control unit activates the heating element 116 to preheat the reactor to the target temperature, for example, setting the temperature between 65°C and 75°C.

[0111] 2. Sample loading: Open the top cover 118 of the device by pressing the corresponding button on the touch screen interface, place the glass slide containing the tissue section on the sample carrier placement rack, start the stepper motor 15 to lower it, so that the top cover 118, the connecting rod 19 and the sample carrier placement rack fall together to the receiving cavity 117 of the reactor body 11, and close the top cover 118.

[0112] 3. Automatic heat repair and dewaxing: Temperature probe 115 monitors the temperature inside the accommodating cavity 117 in real time and adjusts heating element 116 to maintain the set temperature.

[0113] 4. Cooling and Sample Removal: After the heat treatment is completed, heating is stopped, and the control unit instructs the lifting mechanism 14 to gradually lift the top cover 118 via the stepper motor 15, allowing the user to remove the glass slide.

[0114] 5. Equipment cleaning and shutdown: After removing all test samples, the user performs simple cleaning and maintenance, and then shuts down the device via the control unit.

[0115] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A high-pressure immunohistochemistry system, characterized in that: It includes a thermal repair reaction device (1), an incubation device (2), and a flipping mechanism (31); Both the thermal repair reaction device (1) and the incubation device (2) can accommodate the sample carrier (10). The flipping mechanism (31) is used to flip and / or move the sample carrier (10). The flipping mechanism (31) includes a rotating arm (311), a flipping motor (312), and a first suction member (313). The rotating arm (311) is provided with a first suction member (313) for adsorbing the sample carrier (10) at its end. The first suction member (313) is used to adsorb and release the sample carrier (10). The output shaft of the flip motor (312) is connected to the rotating shaft of the rotating arm (311) to drive the rotating arm (311) to rotate.

2. The high-pressure immunohistochemistry system as described in claim 1, characterized in that, The thermal repair reaction device (1) includes: reactor body (11), sample carrier placement rack, reaction driving mechanism, and heating element (116). The reactor body (11) includes a reactor wall and a reactor cover, which form a accommodating cavity (117) for accommodating a sample carrier (10). The reactor cover is connected to a sample carrier placement rack on the side near the accommodating cavity (117); The reactor cover is driven by a reaction-driven mechanism to move closer to or away from the reactor wall; The heating element (116) is disposed on the reactor body (11).

3. The high-pressure immunohistochemistry system as described in claim 2, characterized in that: The sample carrier placement rack is equipped with a limiting component that restricts the vertical placement of the sample carrier (10).

4. The high-pressure immunohistochemistry system as described in claim 1, characterized in that, It also includes an automated sample carrier transfer device, which includes a first robotic arm assembly (3). The first robotic arm assembly (3) includes a robotic arm, a rotating arm (311) mounted on the robotic arm and rotating between the Y and Z directions, and a flip control unit, which is connected to the flip motor (312) in the flip mechanism (31) via a signal.

5. The high pressure immunohistochemistry system of claim 4, wherein: The automatic sample carrier transfer device also includes a third robotic arm assembly (8) and a transfer control unit. The third robotic arm assembly (8) is provided with a second absorbent, which can simultaneously adsorb and transfer more than one sample carrier (10). The transfer control unit controls the rotation of the third robotic arm by connecting to the transfer motor in the third robotic arm assembly (8) via a signal.

6. The high pressure immunohistochemistry system of claim 5, wherein: It also includes a transfer module (9) for accommodating more than one sample carrier (10).

7. The high pressure immunohistochemistry system of claim 1, wherein: It also includes a liquid dispensing device (6), which includes a liquid dispensing body (61) and a reagent assembly and a cleaning assembly disposed on the liquid dispensing body (61); The reagent assembly includes a pipette (621) and a reagent driving mechanism disposed on the liquid addition body (61); The reagent driving mechanism drives the pipette (621) to move up and down relative to the liquid addition body (61), so that the tip of the pipette (621) extends downward and retracts; The cleaning assembly includes a suction tube (631) disposed on the liquid addition body (61). Air blowing pipe (632), liquid outlet pipe (633), cleaning drive mechanism; the cleaning drive mechanism drives the cleaning component to move up and down relative to the liquid adding body (61) Z.

8. The high-pressure immunohistochemistry system as described in claim 1, characterized in that: The incubation device includes an incubation rack, which includes a plurality of parallel incubation trays (23), each incubation tray (23) being connected to an adjacent incubation tray (23) via at least one support structure; each incubation tray (23) can be independently extended.

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