Water sample enrichment and lysis device and water sample DNA monitoring apparatus

Abstract

The utility model discloses a water sample enrichment and cracking device and water sample DNA monitoring equipment, when carrying out water sample DNA monitoring, first through conveying assembly conveying water sample to be measured to make water sample flow through rich crack component, and then through rich crack component filtering water sample to carry out sample enrichment, then through conveying assembly extraction cracking solution to convey cracking solution to rich crack component, and then through rich crack component to the product of enrichment carries out cracking reaction, finally through conveying assembly extraction and convey cracking product to can carry out the subsequent storage, shift and detection of cracking product, through rich crack component and conveying assembly cooperation, through the accurate control to water sample and cracking solution, realized the automatic enrichment and cracking of water sample, relative to prior art, the monitoring process is quick and convenient, and the monitoring result error is small, and the practicality is strong, is suitable for extensive promotion and application.

Description

Technical Field

[0001] This utility model relates to the field of analytical detection technology, and in particular, to a water sample enrichment and lysis device. Furthermore, this utility model also relates to a water sample DNA monitoring device including the aforementioned water sample enrichment and lysis device. Background Technology

[0002] Water sample DNA monitoring (usually referring to the analysis of microbial communities in water bodies based on technologies such as environmental DNA (eDNA) metagenomic sequencing) plays a vital role in water quality and ecosystem health assessment, pathogen detection and public health safety assurance, revealing microbial-driven ecological processes and functions, non-invasive biodiversity surveys and conservation, water resource management, pollution control, environmental governance and ecological restoration.

[0003] The drinking water industry is plagued by odor problems primarily caused by 2-methylisoborneol (MIB). Studies have found that inhibiting the growth of MIB producers in the early stages of an odor event (<15 ng•L⁻¹) can effectively reduce the risk of odor outbreaks (<15 ng•L⁻¹). However, this method requires predicting the risk of an odor outbreak in its early stages. Currently, monitoring the cell density of MIB producers can provide early warning of MIB outbreaks, but at low concentrations, cyanobacterial cells are difficult to observe under a microscope, and cell counting morphologically makes it difficult to distinguish between odor-producing algae (MIB producers) and non-odor-producing algae, making it difficult to achieve targeted and high-quality monitoring of the production status of MIB producers. With the discovery of the metabolic mechanism of MIB, quantitative real-time PCR (qPCR) detection based on MIB synthesis genes, using water sample DNA monitoring, has been proposed as an early warning method for MIB outbreaks and has been validated in practice.

[0004] However, existing water sample DNA monitoring lacks clear and unified monitoring methods and equipment. From sampling, processing, DNA extraction to the application of qPCR detection, a lot of manpower and resources are required. At the same time, different locations, different seasons and different operators can cause errors in monitoring results, resulting in a time-consuming and labor-intensive monitoring process with large errors in monitoring results. Summary of the Invention

[0005] This invention provides a water sample enrichment and lysis device and a water sample DNA monitoring device to solve the technical problems of time-consuming and labor-intensive water sample DNA monitoring process and large error in monitoring results.

[0006] According to one aspect of the present invention, a water sample enrichment and pyrolysis device is provided, comprising: an enrichment and pyrolysis component for filtering water samples to enrich the samples and for pyrolysis reaction of the enriched products; and a conveying component for allowing the water sample to flow through the enrichment and pyrolysis component, conveying the pyrolysis liquid into the enrichment and pyrolysis component, and conveying the pyrolysis products.

[0007] As a further improvement to the above technical solution: Furthermore, the fiber enrichment component includes a robotic arm, a mounting frame, N filters arranged on the mounting frame, a first guide joint arranged on the robotic arm for docking and communicating with the filters, and a second guide joint arranged on the robotic arm for docking and communicating with the filters. Both the first and second guide joints are connected to the conveying component. The robotic arm is used to drive the first and second guide joints to move simultaneously relative to the filters on the mounting frame, or to drive the first and second guide joints to move relative to each other. N is an integer greater than 1.

[0008] Furthermore, the first guide joint and the second guide joint are arranged vertically opposite each other at opposite ends of the robotic arm, and N filters are arranged horizontally at intervals. The openings of the filters are vertical. The first guide joint is used to connect with the upper end of the filter, and the second guide joint is used to connect with the lower end of the filter.

[0009] Furthermore, the first guide joint and the second guide joint are arranged opposite each other at the two ends of the robotic arm in a horizontal direction. N filters are arranged at intervals in a horizontal or vertical direction. The openings of the filters are in the front-to-back direction. The first guide joint is used to connect with the front end of the filter and the second guide joint is used to connect with the rear end of the filter.

[0010] Furthermore, the delivery assembly includes a sampling component for allowing water samples to flow through the pyrolysis unit, a delivery component for delivering pyrolysis fluid into the pyrolysis unit, and a sample delivery component for delivering pyrolysis products.

[0011] Furthermore, the sampling components include a transfer pump connected to the crack-rich component, a sample storage container connected to the transfer pump or the crack-rich component for storing samples, and a level gauge installed on the sample storage container.

[0012] Furthermore, the infusion unit includes a second delivery pump connected to the input end of the rich cracking component, and a fourth multi-way valve connected to the output end of the rich cracking component for opening or closing the output end of the rich cracking component.

[0013] Furthermore, the infusion unit also includes a multi-way valve three connected to the second delivery pump for communicating the pyrolysis fluid. The multi-way valve three is at least a three-way valve and is also used to communicate air so that air can be supplied to compress the pyrolysis fluid after the second delivery pump delivers it.

[0014] According to another aspect of the present invention, a water sample DNA monitoring device is also provided, which includes the above-described water sample enrichment and lysis device.

[0015] As a further improvement to the above technical solution: Furthermore, the water sample DNA monitoring equipment also includes a storage container, a detection instrument for use with the storage container for nucleic acid purification and detection, and a transfer mechanism for transferring the storage container into the detection instrument. The storage container is used to store lysis products, nucleic acid purification reagents, and detection reagents.

[0016] This utility model has the following beneficial effects: This utility model's water sample enrichment and lysis device, when performing DNA monitoring on water samples, first transports the water sample to be tested through a transport component, allowing the water sample to flow through the enrichment and lysis component, where it is then filtered for sample enrichment. Next, the transport component extracts the lysis buffer and transports it to the enrichment and lysis component, where the enriched product undergoes a lysis reaction. Finally, the transport component extracts and transports the lysis product for subsequent storage, transfer, and detection. This solution, through the coordinated operation of the enrichment and lysis component and precise control of the water sample and lysis buffer, achieves automatic enrichment and lysis of water samples. Compared to existing technologies, the monitoring process is fast and convenient, with small monitoring errors, strong practicality, and is suitable for widespread promotion and application.

[0017] In addition to the objectives, features, and advantages described above, this utility model has other objectives, features, and advantages. The present utility model will now be described in further detail with reference to the figures. Attached Figure Description

[0018] The accompanying drawings, which form part of this utility model, are used to provide a further understanding of the utility model. The illustrative embodiments of the utility model and their descriptions are used to explain the utility model and do not constitute an undue limitation of the utility model. In the drawings: Figure 1 This is a first-view structural schematic diagram of the water sample enrichment and pyrolysis device according to a preferred embodiment of the present invention; Figure 2 This is a second-view structural schematic diagram of the water sample enrichment and pyrolysis device according to a preferred embodiment of the present invention; Figure 3 This is a partial structural schematic diagram of the water sample enrichment and pyrolysis device according to a preferred embodiment of the present invention; Figure 4 This is a schematic diagram of the working principle of the water sample enrichment and pyrolysis device according to a preferred embodiment of the present invention; Figure 5 This is a schematic diagram of the structure of a water sample DNA monitoring device according to a preferred embodiment of the present invention.

[0019] Legend: 100. Fiber-enriched component; 110. Robotic arm; 120. Mounting frame; 121. Support plate one; 122. Support plate two; 123. Cover plate; 130. Filter; 140. First guide joint; 150. Second guide joint; 211. Baffle; 212. Heating rod; 220. Vibrating element; 311. Multi-way valve one; 312. Multi-way valve two; 313. Transfer pump one; 314. Sample storage container; 321. Multi-way valve three; 322. Transfer pump two; 323. Multi-way valve four; 331. Transfer pump three; 340. Transfer pump four; 400. Mounting housing; 410. Sampling area; 420. Pretreatment area; 430. Storage area; 440. Detection area; 450. Recovery area; 460. Electrical control area; 510. Driver one; 520. Driver two; 530. Driver three. Detailed Implementation

[0020] The following description provides specific application scenarios and requirements for this specification, intended to enable those skilled in the art to make and use the contents of this specification. Various partial modifications to the disclosed embodiments will be apparent to those skilled in the art, and the general principles defined herein can be applied to other embodiments and applications without departing from the spirit and scope of this specification. Therefore, this specification is not limited to the embodiments shown, but rather to the widest scope consistent with the claims.

[0021] The terminology used herein is for the purpose of describing particular exemplary embodiments only and is not restrictive. For example, unless the context clearly indicates otherwise, the singular forms “a,” “an,” and “the” as used herein may also include the plural forms. When used in this specification, the terms “comprising,” “including,” and / or “containing” mean that the associated integers, steps, operations, elements, and / or components are present, but do not preclude the presence of one or more other features, integers, steps, operations, elements, components, and / or groups, or that other features, integers, steps, operations, elements, components, and / or groups may be added to the system / method.

[0022] Considering the following description, these and other features of this specification, as well as the operation and function of the related components of the structure, and the economy of assembly and manufacture of the parts, can be significantly improved. All of these form part of this specification with reference to the accompanying drawings. However, it should be clearly understood that the drawings are for illustrative and descriptive purposes only and are not intended to limit the scope of this specification. It should also be understood that the drawings are not drawn to scale.

[0023] like Figures 1-4As shown, the water sample enrichment and pyrolysis apparatus of this embodiment includes: an enrichment and pyrolysis component 100, used for filtering water samples to enrich the samples and performing pyrolysis reactions on the enriched products; and a conveying component, used for allowing the water sample to flow through the enrichment and pyrolysis component 100, conveying the pyrolysis liquid into the enrichment and pyrolysis component 100, and conveying the pyrolysis products.

[0024] like Figures 1-4 As shown, specifically, the water sample enrichment and lysis device of this invention, when performing water sample DNA monitoring, firstly transports the water sample to be tested through the transport component, allowing the water sample to flow through the enrichment and lysis component 100, and then filters the water sample through the enrichment and lysis component 100 for sample enrichment; then, the transport component extracts the lysis buffer and transports the lysis buffer into the enrichment and lysis component 100, where the enriched product undergoes a lysis reaction; finally, the transport component extracts and transports the lysis product for subsequent storage, transfer, and detection. This scheme, through the coordinated operation of the enrichment and lysis component 100 and the transport component, and through precise control of the water sample and lysis buffer, achieves automatic enrichment and lysis of the water sample. Compared with existing technologies, the monitoring process is fast and convenient, the monitoring results have small errors, and it is highly practical and suitable for widespread promotion and application.

[0025] like Figure 3 As shown, in this embodiment, the enrichment component 100 includes a robotic arm 110, a mounting frame 120, N filters 130 arranged on the mounting frame 120, a first guide joint 140 arranged on the robotic arm 110 for docking and communicating with the filters 130, and a second guide joint 150 arranged on the robotic arm 110 for docking and communicating with the filters 130. Both the first guide joint 140 and the second guide joint 150 are connected to the conveying component. The robotic arm 110 is used to drive the first guide joint 140 and the second guide joint 150 to move simultaneously relative to the filters 130 on the mounting frame 120, or to drive the first guide joint 140 and the second guide joint 150 to move relative to each other. N is an integer greater than 1.

[0026] like Figure 3 As shown, specifically, the robotic arm 110 drives the first guide connector 140 and the second guide connector 150 to move simultaneously relative to the filter 130 on the mounting frame 120, so that the first guide connector 140, the second guide connector 150 and the filter 130 coincide in a certain direction. Then, the robotic arm 110 moves the first guide connector 140 and the second guide connector 150 closer to each other in this direction, so that the first guide connector 140 and the filter 130 are connected and conductive, and the second guide connector 150 and the filter 130 are connected and conductive, so that the water sample can be enriched and lysed in the current filter 130. By repeating the above steps, the water sample can be enriched and lysed in N filters 130 in sequence, which greatly improves the detection efficiency and realizes effective monitoring of water sample DNA.

[0027] like Figure 3 As shown, in this embodiment, the vibration-enhancing assembly includes a heating element and an oscillating element 220. Specifically, the enriched product is heated by the heating element, and the enriched product is oscillated by the oscillating element 220 to improve the efficiency of the pyrolysis reaction.

[0028] like Figure 3 As shown, in this embodiment, the heating element includes a heating rod 212 and a baffle 211. The heating rod 212 is fixed on the mounting frame 120 by the baffle 211 to heat the enriched product.

[0029] like Figure 3 As shown, in this embodiment, the oscillating element 220 is a DC button motor.

[0030] like Figure 3 As shown, in this embodiment, the oscillating element 220 is arranged on the first guide joint 140 and / or the second guide joint 150, and is arranged away from the heating element to ensure that the oscillating element 220 works normally.

[0031] Optionally, the mounting bracket 120 is mounted on the fixed end of the robotic arm 110.

[0032] like Figure 3 As shown, in this embodiment, the first guide joint 140 and the second guide joint 150 are arranged vertically opposite to each other at opposite ends of the robotic arm 110, and N filters 130 are arranged at intervals in the horizontal direction. The openings of the filters 130 are in the vertical direction. The first guide joint 140 is used to connect with the upper end of the filter 130, and the second guide joint 150 is used to connect with the lower end of the filter 130. Specifically, the robotic arm 110 drives the first guide joint 140 and the second guide joint 150 to move horizontally relative to the mounting frame 120, so that the first guide joint 140, the second guide joint 150 and the filter 130 coincide in the vertical direction. Then, the robotic arm 110 moves the first guide joint 140 and the second guide joint 150 closer to each other in the vertical direction, so that the first guide joint 140 connects with the upper end of the filter 130 and the second guide joint 150 connects with the lower end of the filter 130. During the monitoring process, the water sample and lysis solution are transported by gravity, so that the water sample can be enriched and lysed in the current filter 130, thereby improving the monitoring efficiency.

[0033] Optionally, in another embodiment, the first guide joint 140 and the second guide joint 150 are arranged horizontally opposite each other at opposite ends of the robotic arm 110. N filters 130 are arranged at intervals in a horizontal or vertical direction, with the openings of the filters 130 facing forward and backward. The first guide joint 140 is used to connect with the front end of the filter 130, and the second guide joint 150 is used to connect with the rear end of the filter 130. The robotic arm 110 drives the first guide joint 140 and the second guide joint 150 to simultaneously move vertically or backward relative to the mounting frame 120. The robot moves horizontally so that the first guide joint 140, the second guide joint 150 and the filter 130 coincide in the horizontal direction. Then, the first guide joint 140 and the second guide joint 150 are brought closer to each other in the horizontal direction by the robotic arm 110 so that the first guide joint 140 is connected to the front end of the filter 130 and the second guide joint 150 is connected to the rear end of the filter 130. This allows for the enrichment and pyrolysis of water samples in the current filter 130, and improves the pyrolysis efficiency of water samples in the filter 130, thereby improving monitoring efficiency.

[0034] like Figure 3 As shown, in this embodiment, the mounting frame 120 includes a support plate 121 horizontally arranged on the fixed end of the robotic arm 110, a support plate 122 vertically arranged on the support plate 121, and a cover plate 123 arranged on the support plate 122 for fixing the filter 130.

[0035] In this embodiment, the robotic arm 110 includes a first drive member, a unidirectional lead screw arranged horizontally and connected to the output end of the first drive member, a movable frame sleeved on the unidirectional lead screw, a second drive member arranged on the movable frame, and a bidirectional lead screw arranged vertically and connected to the output end of the second drive member. The two ends of the bidirectional lead screw are respectively connected to a first guide joint 140 and a second guide joint 150. The first drive member operates to drive the unidirectional lead screw to rotate, thereby driving the movable frame to move horizontally relative to the mounting frame 120, thereby driving the first guide joint 140 and the second guide joint 150 to move horizontally relative to the mounting frame 120 at the same time. The second drive member operates to drive the bidirectional lead screw to rotate, thereby driving the first guide joint 140 and the second guide joint 150 to move relative to each other in the vertical direction.

[0036] like Figure 2 and Figure 4As shown, in this embodiment, the delivery assembly includes a sampling component for allowing water samples to flow through the enrichment assembly 100, a delivery component for delivering the pyrolysis solution into the enrichment assembly 100, and a sample delivery component for delivering the pyrolysis products. Specifically, firstly, water samples are extracted through the sampling component in the delivery assembly, allowing the water samples to flow through the enrichment assembly 100, and then the water samples are filtered through the enrichment assembly 100 for enrichment reaction; then, the pyrolysis solution is extracted through the delivery component in the delivery assembly, and the pyrolysis solution is delivered into the enrichment assembly 100, whereby the enriched products are heated and shaken by the vibration component to carry out the pyrolysis reaction; finally, the pyrolysis products are extracted and delivered through the sample delivery component in the delivery assembly, enabling subsequent storage, transfer, and detection of the pyrolysis products.

[0037] like Figure 4 As shown, in this embodiment, the sampling component includes a transfer pump 313 connected to the crack enrichment component 100, a sample storage container 314 connected to the transfer pump 313 or the crack enrichment component 100 for storing samples, and a level gauge installed on the sample storage container 314. Specifically, the transfer pump 313 operates to extract samples, allowing the samples to flow sequentially through the crack enrichment component 100 and the transfer pump 313, thereby being transported into the sample storage container 314 to achieve sample enrichment. The level gauge detects and provides feedback on the liquid level in the sample storage container 314, so that after the set liquid level is increased, the transfer pump 313 is controlled to stop operating, thus achieving quantitative extraction of the sample.

[0038] Alternatively, in another embodiment, a transfer pump 313 is used to extract a sample, which flows sequentially through the transfer pump 313 and the enrichment component 100, and is then transported to a sample storage container 314 to enrich the sample. The liquid level in the sample storage container 314 is detected and fed back by a level gauge, so that the transfer pump 313 stops working after the set liquid level is increased, thus realizing quantitative extraction of the sample.

[0039] It should be understood that the liquid level setting can be adaptively set according to monitoring needs.

[0040] like Figure 4 As shown, in this embodiment, the infusion unit includes a second transfer pump 322 connected to the input end of the pyrolysis component 100, and a fourth multi-way valve 323 connected to the output end of the pyrolysis component 100 for opening or closing the output end of the pyrolysis component 100. Specifically, during the pyrolysis reaction, the output end of the pyrolysis component 100 is first closed through the fourth multi-way valve 323, the second transfer pump 322 operates to draw up the pyrolysis liquid, and the pyrolysis liquid flows sequentially through the second transfer pump 322 to flow into the pyrolysis component 100 for the pyrolysis reaction.

[0041] In this embodiment, the multi-way valve 323 is connected to the second guide connector 150.

[0042] Optionally, the transfer pump 2322 is a ceramic pump.

[0043] Optionally, the multi-way valve 4323 is a two-way valve, which is open under normal conditions and closed during the cracking reaction.

[0044] In this embodiment, the conveying assembly also includes a fourth conveying pump 340, which is used as a backup for quick replacement in case other pumps fail.

[0045] like Figure 4 As shown, in this embodiment, the infusion unit also includes a multi-way valve 321 connected to the second transfer pump 322 for communicating the pyrolysis solution. The multi-way valve 321 is at least a three-way valve and is also used to communicate air, so that after the second transfer pump 322 delivers the pyrolysis solution, air is then delivered to compress the pyrolysis solution. Specifically, after the second transfer pump 322 delivers a quantitative amount of pyrolysis solution through the multi-way valve 321, the multi-way valve 321 is connected to air, so that air is drawn out by the second transfer pump 322, causing the pyrolysis solution to accumulate in the pyrolysis enrichment component 100, ensuring sufficient pyrolysis reaction, improving pyrolysis efficiency, and reducing the amount of pyrolysis solution used, thus reducing monitoring costs.

[0046] like Figure 4 As shown, in this embodiment, the sampling component also includes a multi-way valve 311 for connecting the water sample, which is connected to the second transfer pump 322 and the pyrolysis enrichment component 100 respectively. The multi-way valve 311 integrates the input pipelines of the water sample and the pyrolysis solution, simplifying the structure of the transfer component. During sample enrichment, the water sample is transferred to the pyrolysis enrichment component 100 under the action of the second transfer pump 313; during the pyrolysis reaction, the pyrolysis solution is transferred to the pyrolysis enrichment component 100 under the action of the second transfer pump 322.

[0047] In this embodiment, the multi-way valve 311 is a three-way valve and is connected to the first guide joint 140.

[0048] like Figure 4 As shown, in this embodiment, the sample delivery component includes a transfer pump 331 connected to the pyrolysis enrichment component 100 for transporting pyrolysis products. Specifically, after the pyrolysis reaction is complete, the transfer pump 331 operates to output the pyrolysis products in the pyrolysis enrichment component 100 via the transfer pump 331, thereby enabling subsequent storage, transfer, and detection of the pyrolysis products.

[0049] like Figure 4 As shown, in this embodiment, the sampling component also includes a multi-way valve 312 connected to the multi-way valve 323, the transfer pump 313 and the transfer pump 331 respectively. The multi-way valve 312 integrates the output pipelines of water sample and pyrolysis products, simplifying the structure of the delivery component.

[0050] The working process of the conveyor component is as follows: After the water sample is connected to multi-port valve 311, transfer pump 313 operates to extract the sample. The sample flows sequentially through multi-port valve 311, first guide connector 140, filter 130, second guide connector 150, multi-port valve 312, and transfer pump 313, thus being transported to the sample storage container 314 for sample enrichment. The liquid level in the sample storage container 314 is detected and fed back by a level gauge. When the set liquid level is reached, transfer pump 313 is stopped, achieving quantitative sample extraction. After the lysis solution is connected to multi-port valve 321, multi-port valve 311 and transfer pump 322 are connected. When multi-way valve 4 323 is closed, transfer pump 2 322 operates to extract the pyrolysis liquid and allow it to flow sequentially through multi-way valve 3 321, transfer pump 2 322, multi-way valve 1 311, and first guide joint 140, thereby delivering it to filter 130 for pyrolysis reaction. After the pyrolysis reaction is complete, multi-way valve 4 323 opens, and transfer pump 3 331 operates, allowing the pyrolysis products in filter 130 to flow sequentially through second guide joint 150, multi-way valve 4 323, multi-way valve 2 312, and transfer pump 3 331 to transport the pyrolysis products, thereby enabling subsequent storage, transfer, and detection of the pyrolysis products.

[0051] like Figure 2 As shown, in this embodiment, the water sample enrichment and pyrolysis device further includes a driver 510 connected to the sampling component, a driver 520 connected to the infusion component, and a driver 530 connected to the sample delivery component. Specifically, the driver 510 controls the operation of the sampling component to achieve quantitative delivery of the water sample to be tested; the driver 520 controls the operation of the infusion component to achieve quantitative extraction of the pyrolysis solution; and the driver 530 controls the operation of the sample delivery component to achieve quantitative delivery of the pyrolysis products.

[0052] like Figure 5 As shown, the water sample DNA monitoring device of this embodiment includes the aforementioned water sample enrichment and lysis device. Specifically, by employing the aforementioned water sample enrichment and lysis device in the water sample DNA monitoring device, and through the coordinated operation of the enrichment and lysis component 100 and the transport component, and through precise control of the water sample and lysis buffer, automatic enrichment and lysis of the water sample are achieved. This makes the monitoring process quick and convenient, the monitoring results have small errors, and it is highly practical and suitable for widespread promotion and application.

[0053] like Figure 5As shown in this embodiment, the water sample DNA monitoring device also includes a storage container, a detection instrument for cooperating with the storage container to perform nucleic acid purification and detection, and a transfer mechanism for transferring the storage container to the detection instrument. The storage container is used to store lysis products, nucleic acid purification reagents, and detection reagents. Specifically, after the sample enrichment and lysis reactions are completed sequentially, the lysis products are transported to the storage container by a transfer pump 331, and then the storage container is transferred to the detection instrument by the transfer mechanism, so that the nucleic acid purification and detection of the lysis products can be performed by the detection instrument, thereby realizing the detection of water sample DNA.

[0054] Optionally, the transfer mechanism is a three-axis robotic arm.

[0055] It should be understood that the specific structure of the three-axis robotic arm is a technology known to those skilled in the art.

[0056] Optionally, multiple storage containers are provided, and multiple pyrolysis products are stored in multiple storage containers.

[0057] Optionally, the water sample DNA monitoring equipment also includes a recycling container, which is used to transfer the storage container inside the testing instrument to the recycling container after the testing is completed.

[0058] Optionally, the water sample DNA monitoring equipment also includes a control component, which controls the coordinating operation of the enrichment and lysis component 100, the vibration component, the transport component, the detection instrument, and the transfer mechanism to achieve full automation of the water sample enrichment, lysis, sample transfer, and sample detection process.

[0059] like Figure 5 As shown, optionally, the water sample DNA monitoring equipment also includes a housing 400, a fission enrichment component 100, a vibration amplification component, a transport component, a storage container, a detection instrument, a transfer mechanism, a recovery container, and a control component, all of which are arranged inside the housing 400 to ensure that the monitoring process is carried out in a closed environment, thereby reducing the error of the monitoring results.

[0060] like Figure 5 As shown, optionally, the inner cavity of the mounting housing 400 is divided into a sampling area 410, a pretreatment area 420, a storage area 430, a detection area 440, a recovery area 450, and an electronic control area 460. The sampling area 410 is equipped with a sample rack, a sampling needle, and a quantitative cup. A magnetic stirring mechanism is installed on the sample rack. The crack enrichment component 100, the vibration enhancement component, and the conveying component are installed in the pretreatment area 420. The storage container is installed in the storage area 430. The detection instrument is installed in the detection area 440. The recovery container is installed in the recovery area 450. The control component is installed in the electronic control area 460.

[0061] The foregoing has described specific embodiments of this specification. Other embodiments are within the scope of the appended claims. In some cases, the actions or steps recited in the claims may be performed in a different order than that shown in the embodiments and may still achieve the desired result. Furthermore, the processes depicted in the drawings do not necessarily require a specific or sequential order to achieve the desired result. In some embodiments, multitasking and parallel processing are possible or may be advantageous.

[0062] In summary, after reading the detailed disclosure of this specification, those skilled in the art will understand that the foregoing detailed disclosure is presented by way of example only and is not restrictive. Although not explicitly stated herein, those skilled in the art will understand that this specification requires various reasonable changes, improvements, and modifications to the embodiments. These changes, improvements, and modifications are intended to be made by this specification and are within the spirit and scope of the exemplary embodiments described herein.

[0063] Furthermore, certain terms in this specification have been used to describe embodiments of this specification. For example, "an embodiment," "an embodiment," and / or "some embodiments" mean that a particular feature, structure, or characteristic described in connection with that embodiment may be included in at least one embodiment of this specification. Therefore, it is to be emphasized and understood that two or more references to "an embodiment" or "an embodiment" or "alternative embodiment" in various parts of this specification do not necessarily refer to the same embodiment. Moreover, specific features, structures, or characteristics may be suitably combined in one or more embodiments of this specification.

[0064] It should be understood that in the foregoing description of the embodiments in this specification, various features are combined in a single embodiment, drawing, or description for the purpose of simplifying the description and aiding in the understanding of a feature. However, this does not mean that the combination of these features is necessary, and those skilled in the art may readily identify some of the devices as separate embodiments when reading this specification. That is, the embodiments in this specification can also be understood as an integration of multiple secondary embodiments. It is also valid when each secondary embodiment contains fewer than all the features of a single foregoing disclosed embodiment.

[0065] Each patent, patent application, publication of the patent application, and other materials such as articles, books, specifications, publications, documents, articles, etc., cited herein may be incorporated by reference. The entire contents used for all purposes, except for any history of prosecution documents associated with it, that may be inconsistent with or conflict with this document, or that may have a limiting effect on the widest extent of the claims, are now or hereafter associated with this document. For example, in the event of any inconsistency or conflict between the description, definition, and / or use of terms associated with any of the included materials and the terms, description, definition, and / or used in connection with this document, the terms used herein shall prevail.

[0066] Finally, it should be understood that the embodiments disclosed herein are illustrative of the principles of the embodiments described in this specification. Other modified embodiments are also within the scope of this specification. Therefore, the embodiments disclosed in this specification are merely examples and not limitations. Those skilled in the art can implement the applications described in this specification using alternative configurations based on the embodiments in this specification. Therefore, the embodiments in this specification are not limited to the embodiments precisely described in the applications.

Claims

1. A water sample enrichment and pyrolysis device, characterized in that, include: A pyrolysis unit (100) is used to filter water samples for sample enrichment and to perform pyrolysis reactions on the enriched products. A delivery assembly for allowing water samples to flow through the crack enrichment assembly (100), delivering pyrolysis liquid into the crack enrichment assembly (100), and delivering pyrolysis products.

2. The water sample enrichment and pyrolysis device according to claim 1, characterized in that, The fiber enrichment assembly (100) includes a robotic arm (110), a mounting frame (120), N filters (130) arranged on the mounting frame (120), a first guide joint (140) arranged on the robotic arm (110) for connecting with the filters (130), and a second guide joint (150) arranged on the robotic arm (110) for connecting with the filters (130). The first guide joint (140) and the second guide joint (150) are both connected to the conveying assembly. The robotic arm (110) is used to drive the first guide joint (140) and the second guide joint (150) to move simultaneously relative to the filters (130) on the mounting frame (120), or to drive the first guide joint (140) and the second guide joint (150) to move relative to each other. N is an integer greater than 1.

3. The water sample enrichment and pyrolysis device according to claim 2, characterized in that, The first guide joint (140) and the second guide joint (150) are arranged vertically opposite to each other at opposite ends of the robotic arm (110). N filters (130) are arranged horizontally at intervals. The opening of the filters (130) is in the vertical direction. The first guide joint (140) is used to connect with the upper end of the filter (130) and the second guide joint (150) is used to connect with the lower end of the filter (130).

4. The water sample enrichment and pyrolysis device according to claim 2, characterized in that, The first guide joint (140) and the second guide joint (150) are arranged horizontally at opposite ends of the robotic arm (110). N filters (130) are arranged at intervals in the horizontal or vertical direction. The opening of the filter (130) is in the front-to-back direction. The first guide joint (140) is used to connect with the front end of the filter (130), and the second guide joint (150) is used to connect with the rear end of the filter (130).

5. The water sample enrichment and pyrolysis apparatus according to any one of claims 1-4, characterized in that, The delivery assembly includes a sampling device for allowing water samples to flow through the pyrolysis unit (100), a delivery device for delivering pyrolysis fluid into the pyrolysis unit (100), and a sample delivery device for delivering pyrolysis products.

6. The water sample enrichment and pyrolysis device according to claim 5, characterized in that, The sampling components include a transfer pump (313) connected to the crack-rich assembly (100), a sample storage container (314) connected to the transfer pump (313) or the crack-rich assembly (100) for storing samples, and a level gauge disposed on the sample storage container (314).

7. The water sample enrichment and pyrolysis device according to claim 5, characterized in that, The infusion unit includes a second delivery pump (322) connected to the input end of the rich cracking assembly (100), and a fourth multi-way valve (323) connected to the output end of the rich cracking assembly (100) for opening or closing the output end of the rich cracking assembly (100).

8. The water sample enrichment and pyrolysis device according to claim 7, characterized in that, The infusion unit also includes a multi-way valve three (321) connected to the second (322) for communicating the pyrolysis fluid. The multi-way valve three (321) is at least a three-way valve and is also used to communicate air so as to deliver air-compressed pyrolysis fluid after the second (322) has delivered the pyrolysis fluid.

9. A water sample DNA monitoring device, characterized in that, Includes the water sample enrichment and pyrolysis apparatus according to any one of claims 1-8.

10. The water sample DNA monitoring device according to claim 9, characterized in that, The water sample DNA monitoring equipment also includes a storage container, a detection instrument for use with the storage container for nucleic acid purification and detection, and a transfer mechanism for transferring the storage container into the detection instrument. The storage container is used to store lysis products, nucleic acid purification reagents, and detection reagents.

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