Proteins and nanostructures of respiratory syncytial virus and their uses

Abstract

Recombinant polypeptides comprising a modified ectodomain of a respiratory syncytial virus (RSV) fusion protein (F) are provided herein, wherein the ectodomain comprises a modified C-terminus alpha-helical segment and / or amino acid substitutions that stabilize the F protein in a prefusion conformation. A two-component protein nanostructure comprising a first trimeric component and a second pentameric component is also provided herein. A composition for use in vaccination, elicitation of an immune response, or treatment or prevention of RSV disease is provided herein.