METHOD FOR THE QUALITATIVE AND / OR QUANTITATIVE DETECTION OF SUBSTANCES CONTAINED IN A HEMP PLANT AND KIT FOR USE THEREIN

Description

[0001] The present invention relates to a method for the qualitative and / or quantitative detection of substances contained in the hemp plant. The invention further relates to a kit for use in this method. BACKGROUND OF THE INVENTION

[0002] WO 2012 / 154306 A1 describes a kit comprising a) an ampoule; b) a material comprising a hemp plant or parts thereof; and c) a color indicator capable of reacting with the hemp plant and / or at least a part thereof to change the color of the color indicator, wherein the material and the color indicator are arranged in the ampoule.

[0003] The legalization of cannabis and its use as a medicine raises many questions regarding quality assurance by producers and distributors, especially pharmacists and physicians. In particular, the pharmacist must ensure and verifiably document, in accordance with pharmaceutical regulations, the active ingredient content of a marketed product.

[0004] Information on the THC and CBD content is particularly problematic. There are no fixed upper and lower limits for the content of these key substances, but they should be known or able to be determined. The content of a batch should be within a range of + / - 10 percent of the content declared on the packaging. This is due to the fact that individual varieties differ considerably in their tetrahydrocannabinol (THC) and cannabidiol (CBD) content. The fact that there can also be significant differences from harvest to harvest within a single variety is not taken into account. From a pharmaceutical perspective, this is unsatisfactory, especially given that changed and stricter regulations are expected in the future.

[0005] EP 1 32 313 A2 describes cannabinoid detection methods for drug test kits based on diazonium reagents. The reaction is carried out on filter paper, and the reagents are applied in liquid form or as a spray. These and similar methods are relatively cumbersome to perform. Furthermore, preparation of the sample material through measures such as drying, extraction, filtration, and evaporation is necessary to carry out the corresponding determinations. This requires considerable labor and time and can sometimes lead to a change in the material composition of the sample material. Furthermore, these methods often require the use of toxic or highly corrosive chemicals, which is problematic for widespread application by laypersons.

[0006] It is therefore the object of the present invention to provide a method and the associated kit for the qualitative and / or quantitative detection of substances contained in hemp plants, which overcomes disadvantages of the prior art, in particular to provide a method and the associated kit that allows the simple and cost-effective analysis of substances that may be contained in hemp plants or other hemp-based products, in particular by persons who are inexperienced in chemical analysis, such as specialists, pharmacists, doctors, hospital staff, etc. The method according to the invention and the associated kit should also allow analytically inexperienced persons, such as pharmacists, to determine the active ingredient content in hemp-based products with an accuracy that satisfies the current requirements. DESCRIPTION OF THE INVENTION

[0007] This object is achieved by a kit for the qualitative and / or quantitative detection of one or more substances contained in the hemp plant, comprising: a) an ampoule which is transparent and evacuated and consists at least partially of borosilicate glass; b) a material which comprises a hemp plant or parts thereof; and c) a color indicator which is suitable for reacting with the hemp plant and / or at least a part thereof by changing the color of the color indicator, wherein the material and the color indicator are arranged in the airtight and evacuated ampoule.

[0008] The method according to the invention allows for a simple qualitative and quantitative quality testing of hemp- or cannabis-based materials prior to marketing. This testing can be performed easily and safely even by an untrained person, such as a pharmacist, who can then guarantee and certify the quality of the product they distribute.

[0009] The material comprises a hemp plant or a part thereof. A part of the hemp plant can also be understood as a single chemical compound isolated from the hemp plant, preferably a pharmaceutically active compound.

[0010] Cannabis (hemp), along with the genus Humulus (hops), belongs to the Cannabisaceae family, although Humulus does not contain cannabinoids. Within the genus Cannabis, a botanical and chemotaxonomic differentiation occurs, namely into the species Cannabis sativa Linnaeus, Cannabis indica LAM, and Cannabis ruderalis, or into the "collective species" Cannabis sativa L., consisting of the subspecies Cannabis sativa ssp. sativa and ssp. indica. Furthermore, cannabis is divided into drug hemp and fiber hemp, with the distinction being based on the quantitative ratio of the main cannabinoids cannabidiol (CBD) and Δ<-tetrahydrocannabinol (Δ<-THC) (INN: dronabinol). Fiber hemp (also: commercial hemp, industrial hemp) is mainly used for industrial fiber production and may have a maximum Δ 9< -THC content of 0.2% (e.g. Germany, etc.), while the drug type can have a Δ 9< -THC content of approx. 5-15% (marijuana, hashish).Cannabis sativa L. contains over 400 different compounds, of which more than 60 belong to the class of cannabinoids. The most important cannabinoids are listed below: ∘ Cannabigerol-like (CBG) : cannabigerol ((E)-CBG-C 5 ), cannabigerol monomethyl ether ((E)-CBGM-C 5 A), cannabigerolic acid A ((Z)-CBGA-C 5 A), cannabigerovarin ((E)-CBGV-C 3 ), cannabigerolic acid A ((E)-CBGA-C 5 A), cannabigerolic acid A monomethyl ether ((E)-CBGAM-C 5 A), cannabigerovaric acid A ((E)-CBGVA-C 3 A); ∘ Cannabichromene-like (CBC) : cannabichromene (CBC-C 5 ), cannabichromenic acid A (CBCA-C 5 A), cannabichromevarin (CBCV-C 3 ), cannabichromevarinic acid A (CBCVA-C3 A); ∘ Cannabidiol (CBD): Cannabidiol (CBD-C 5 ), Cannabidiol monomethyl ether (CBDM-C 5 ), Cannabidiol-C4 (CBD-C 4 ), Cannabidivarin (CBDV-C 3 ), Cannabidiorcol (CBD-C 1 ), Cannabidiolic acid (CBDA-C 5 ), Cannabidivarinic acid (CBDVA-C 3 ); ∘ Cannabinodiol-like (CBND) : cannabinodiol (CBND-C 5 ), cannabinodivarin (CBND-C 3 ); ∘ Tetrahydrocannabinol-like (THC):Δ9-Tetrahydrocannabinol (Δ9-THC-C 5 ), Δ9-Tetrahydrocannabinol-C4 (Δ9-THC-C 4 ), Δ9-Tetrahydrocannabivarin (Δ9-THCV-C 3 ), Δ9-Tetrahydrocannabiorcol (Δ9-THCO-C 9 Δ9-Tetrahydrocannabinolsäure (Δ9-THCA-C 5 A), Δ9-Tetrahydrocannabinolsäure B (Δ9-THCA-C 5 B), Δ9-Tetrahydrocannabinolsäure-C4 (Δ9-THCAins-C 4 A und / oderbi Ahydra BTevar Δ9 (Δ9-THCVA-C 3 A), Δ9-Tetrahydrocannabiorcolsäure (Δ9-THCOA-C 1 A und / oder B), (-)-Δ8-trans-(6aR,10aR)-Δ8-Tetrahydrocannabinol (Δ8-THC-C 5 ),(-)-Δ8-trans-(6aR,10aR)-Tetrahydrocannabinolsäure A (Δ8-THCA-C 5 A); (-)-(6aS,10aR)-Δ9-Tetrahydrocannabinol ((-)-cis-Δ9-THC-C 5 ); ∘ Cannabinol-like (CBN): Cannabinol CBN-C 5 , Cannabinol-C4 (CBN-C 4 ), Cannabivarin (CBN-C 3 ), Cannabinol-C2 (CBN-C 2 ), Cannabiorcol (CBN-C 1 ), Cannabinolsäure A (CBNA-C 5 A), Cannabinolmethylether (5CBNM ) Cannabitriol-like (CBT): (-)-(9R,10R)-trans-Cannabitriol ((-)-trans-CBT-C 5 ), (+)-(9S,10S)-Cannabitriol ((+)-trans-CBT-C 5 ), (±)-(9R,10S / 9S,10R)-Cannabitriol ((+)-cis-CBT-C 5 ), (-)-(9R,10R)-trans[10-o-Ethyl-cannabitriol] ((-)-trans-CBT-OEt-C 5 ), (±)-(9R,10R / 9S,1oS)-Cannabitriol-C3 ((±)-trans-CBT-C 3 ),8,9-Dihydroxy-Δ6a(10a) tetrahydrocannabinol (8,9-Di-OH-CBT-C 5 ), Cannabidiolsäure A (CBDA-C 5 9-OH-CBT-C5 ester), (-)-(6aR,9S,10S,10aR)-9,10-Dihydroxy-hexahydrocannabinol, Cannabiripsol Cannabiripsol-C5, (-)-6a,7,10a-Trihydroxy-Δ9-tetrahydrocannabinol ((-)-Cannabitetrol), 10-Oxo-Δ6a(10a) tetrahydrocannabinol (OTHC); ∘ Cannabielsoin-like (CBE) : (5aS, 6S, 9R, 9aR) - C 5 -Cannabielsoin (CBE-C 5 ), (5aS,6S,9R,9aR)-C 3 -Cannabielsoin (CBE-C 3 ), (5aS,6S,9R,9aR)-Cannabielsoinsäure A (CBEA-C 5 A), (5aS,6S,9R,9aR)-Cannabielsoinsäure B (CBEA-C 5 B), (5aS,6S,9R,9aR)-C3-Cannabielsoinsäure B (CBEA-C 3 B), Cannabiglendol-C3 (OH-iso-HHCV-C 3 ), Dehydrocannabifuran (DCBF-C 5 ), Cannabifuran (CBF-C 5 ) ; ∘ Isocannabinoid:(-)-Δ7-trans-(1R,3R,6R)-Isotetrahydrocannabinol, (±)-Δ7-1,2-cis-(1R,3R,6S / 1S,3S,6R)-Isotetrahydrocannabivarin, (-)-Δ7-trans-(1R,3R,6R)-Isotetrahydrocannabivarin; ∘ Cannabicyclol-like (CBL) : (±)-(1aS,3aR,8bR,8cR-cannabicyclol (CBL-C 5 ), (±)-(1aS,3aR,8bR,8cR-cannabicyclolic acid A (CBLA-C 5 A), (±)-(1aS,3aR,8bR,8cR-cannabicyclovarin (CBLV-C 3 ); ∘ Cannabidiol (CBT) : Cannabicitran (CBT-C 5 ) ; ∘ Cannabichromanone-like (CBCN) : cannabichromanone (CBCN-C 5 ), cannabichromanone-C3 (CBCN-C 3 ), cannabicoumaronone (CBCON-C 5 ).

[0011] In addition to the cannabinoids mentioned above, their corresponding carboxylic acids are found in the raw drug. These carboxylic acids are biosynthetic precursors.

[0012] Cannabis preparations exert a variety of therapeutic effects, including antispastic, analgesic, antiemetic, neuroprotective, anti-inflammatory and effects in psychiatric disorders (Grotenhermen F, Müller-Vahl K: The therapeutic potential of cannabis and cannabinoids.

[0013] In Germany, a cannabis extract containing THC (dronabinol) and CBD in a 1:1 ratio (nabiximols) has been approved under pharmaceutical law since 2011 for the treatment of moderate to severe, therapy-resistant spasticity in multiple sclerosis (MS) as a sublingual spray (Sativex).

[0014] Cannabidiol (CBD, CBD-C 5 ) is the most important non-psychotropic cannabinoid of the genus Cannabis and CBD is not a cannabinoid receptor agonist. Fig. 1: CBD (structural formula)

[0015]

[0016] CBD can be produced synthetically (Michoulam R, Shvo Y., Hashish. I. The structure of cannabidiol, Tetrahedron. 1963, 19(12), 2073).

[0017] In one embodiment, the material may comprise a cannabis flower, marijuana, hashish, hashish oil, at least one cannabinoid, or a mixture thereof.

[0018] In the various embodiments, the kit according to the invention (as well as the method according to the invention described below) can be used for the identification and quality determination of cannabis preparations such as marijuana, hashish, hashish oil and other cannabinoid-containing materials, for determining the degree of maturity of hemp plants, as well as for distinguishing between drug and young hemp plants in fresh or dried form.

[0019] In particular, it can be provided that the kit / method according to the invention serves for the chemical detection of various phenol-like compounds, essential oils, resinoids, fresh plants, plant drugs, plant extracts and extracts and odorous substances, as well as for their identification and characterization and quality determination.

[0020] In a further embodiment, it can be provided that the color indicator comprises a color-forming substance which is preferably selected from the group consisting of fast black, fast blue salt, dibromoquinone chloroimide, dichloroquinone chloroimide, vanillin, salicylaldehyde, formaldehyde, acetaldehyde, p-dimethylaminobenzaldehyde, diethylaminobenzaldehyde, iron(III) chloride, aminophenol, aminoantipyrine, potassium hexacyanoferrate and a mixture of two or more thereof.

[0021] It can also be provided that the color indicator comprises at least one solvent, which is preferably selected from the group consisting of water and mono- or polyhydric alcohols.

[0022] In particular, it can be provided that monohydric or polyhydric alcohols alone or mixtures thereof are used as solvents, which on the one hand provide optimal extraction of the active ingredients from the material and on the other hand also provide good dissolving properties for the color-forming substance used and, if applicable, other components of the color indicator and guarantee a trouble-free color reaction.

[0023] In a further embodiment, it can be provided that the color indicator further comprises a reagent that supports the reaction of the material with the color-forming substance, wherein the reagent is preferably a basic compound, particularly preferably selected from the group consisting of alkali hydroxide, alkali carbonate, ammonium or alkali salts of an organic acid and a mixture of two or more thereof.

[0024] Furthermore, the color indicator may further comprise a carrier material, preferably an absorbent, neutral carrier material. The carrier material may be open-pored or closed-pored. The individual components of the color indicator, such as the coloring substance or the reagent, may be contained in the pores.

[0025] In one embodiment, it can be provided that the color indicator is composed of a first solution of the color-forming substance in water and / or primary, secondary and / or tertiary alcohols and a second solution of a base, for example alkali hydroxide, in water or alcohols or mixtures thereof.

[0026] According to the invention, the components contained in the color indicator react, alone or together with a change in the color of the color indicator, to the presence of substances contained in the material according to the invention.

[0027] In a further embodiment, it may be provided that the ampoule is a divisible ampoule consisting of two or more parts that can be joined together to form the ampoule, wherein the color indicator is arranged on at least part of an inner wall of one of the parts that can be joined together to form the ampoule.

[0028] According to the invention, the ampoule is hermetically and / or hermetically sealed.

[0029] For example, it can be provided that the ampoule consists of three different parts that are connected to one another in an airtight manner. The connection of the individual parts of the ampoule can be achieved in various ways, for example by screwing the parts together. It can be provided that the color indicator is arranged on an inner wall (or a part thereof) of one part (or several parts) of the divisible ampoule. The inner wall is the part that is arranged towards the interior of the ampoule after the parts have been assembled and connected. It can be provided that the carrier is arranged on the inner wall of the ampoule or a part thereof, for example by an adhesive connection between the carrier and the inner wall.The remaining components of the color indicator, in particular the color-forming substance, are then arranged on the carrier, towards the inside of the ampoule, in order to be brought into contact with the material or components released from this material, for example with substances released from the hemp plant.

[0030] In one embodiment, it can be provided that the part on whose inner wall the color indicator is at least partially arranged is a spacer disc which can be connected by screwing to one or more other parts of the divisible ampoule.

[0031] In one embodiment, it can be provided that the individual components of the divisible ampoule comprise disc-shaped screwable rings that can be connected to one another on both sides.

[0032] In a further embodiment, it can be provided that the ampoule, in particular the interior of the ampoule, is corrosion-resistant, in particular against acids and bases.

[0033] Since the ampoule is transparent and at least partially made of borosilicate glass, the color change of the color indicator can be easily evaluated visually, and if necessary, stored accordingly and compared with data stored in a database as a reference.

[0034] According to the invention, the ampoule is evacuated. The object is further achieved by a method for the qualitative and / or quantitative detection of one or more substances contained in the hemp plant, comprising the steps: a) providing the kit according to the invention; b) contacting the material or parts thereof with the color indicator; and c) detecting a change in the color of the color indicator. Bringing the material or parts thereof into contact with the color indicator comprises heating the material or parts thereof in the transparent, evacuated ampoule, which is made at least partially of borosilicate glass.

[0035] It is contemplated that bringing the material or parts thereof into contact with the color indicator comprises heating the material or parts thereof in the ampoule. In this context, it may be contemplated that the heating comprises inductive heating.

[0036] In a further embodiment, it may be provided that the detection is carried out using a color comparison scale, an optical sensor, a chemical sensor or two or more thereof.

[0037] It can also be provided that the method further comprises a step, after the detection, comprising comparing information obtained by the detection with data stored in a database. DETAILED DESCRIPTION OF THE INVENTION

[0038] The invention will be described below with reference to the drawings using specific embodiments. It should be understood that the reference to the specific embodiments serves merely to illustrate the invention. The features of the specific embodiments are not necessarily limiting for the invention, but can, particularly in combination with the aforementioned embodiments, contribute to the advantageous realization of the invention. Fig.1 : schematic representation of an ampoule according to an embodiment of the invention; Fig.2 : schematic representation of an ampoule according to a further embodiment of the invention; and Fig.3 : schematic representation of an ampoule according to another embodiment of the invention.

[0039] A method for quality testing of pharmaceutical cannabis is described, in which an ampoule containing a material, such as cannabis flowers or parts thereof, can be designed in such a way that the ampoule itself or by combining it with additional elements ensures the appropriate tests for placing on the market by the pharmacist and test certificates can be verifiably documented and issued in accordance with pharmaceutical regulations.

[0040] According to the invention, the cannabis flowers or parts thereof are hermetically packaged immediately after harvest and sealed in a special transparent ampoule made of borosilicate glass in such a way that the active ingredient can only be extracted by breaking the ampoule. A chemical-analytical examination can be carried out within the evacuated ampoule using various tools described herein. The results can be applied as data to corresponding barcodes or similar. Other relevant information, such as cultivation, harvesting, processing, quality control, storage, and packaging, is of course also available in a tamper-proof manner. This can be achieved by burning a laser code into the surface. The data can be read out in a docking station.

[0041] With the new method, which is described in more detail in the claims, it is possible to conduct quality tests even during the plant's growth. It is conceivable that the cannabis flower in the vial could be examined during growth.

[0042] Chemical tests are performed in the ampoule. The ampoule is designed so that the test elements and corresponding reagents are either part of the ampoule or can be screwed onto the ampoule.

[0043] The kit described in the claims ensures compliance with the usual requirements, in particular: consistent product quality substances contained information on the concentration of the active ingredients avoidance of any kind of contamination traceability

[0044] The kit and method according to the invention enable specialists, pharmacists, physicians or clinical staff to chemically detect phenol-like substances, in particular cannabinoids and materials containing such substances.

[0045] The sample can be directly mixed with a color-forming reagent, e.g. in pretreated or untreated form, without prior drying. This can be achieved by applying a Figure 1 The multi-part ampoule 100 shown comprises a screw-on annular adapter 110. The annular adapter 110 contains, in its area filling the ring, the color indicator required for the analysis of the material 120.

[0046] The color indicator can be used as a solution of a color-forming substance and the reagent with or without the addition of organic solvents.

[0047] The color-forming substance is preferably presented in the form of dilute solutions of the color-forming substance in water and / or primary, secondary and tertiary alcohols with or without the addition of organic solvents such as saturated and unsaturated hydrocarbons, halogenated hydrocarbons, ethers, ketones, carboxylic acid esters and / or aromatic hydrocarbons.

[0048] The reagent is preferably a base, such as an alkali metal hydroxide and / or an alkali metal carbonate, an ammonium or alkali metal salt of an organic acid, optionally substituted by one or more organic radicals, e.g., alkyl groups, or mixtures thereof, and can be present in the form of a dilute solution together with the color-forming substance in water and / or primary, secondary, and alcohols or mixtures thereof. Ammonium or alkali metal salts of organic acids such as acetic acid, propionic acid, butyric acid, malic acid, sorbic acid, fumaric acid, benzoic acid, phenylacetic acid, phthalic acid, naphthylacetic acid, or mixtures thereof can optionally be used.

[0049] Surprisingly, it has been possible to develop a process in which specially selected color reactions are modified in such a way that the components relevant for the color reaction are added directly to the solvent acting as the extraction agent and are used directly in the chemical detection reaction. Figure 2 In the ampoule 200 shown, this is achieved by pressing the test material (not shown), in this case cannabis flowers, into the ampoule 200 between a first spacer ring 210 and a second spacer ring 220. In the Figure 2a the ampoule is shown in the non-compressed state, whereas the Figure 2b shows the same ampoule in the compressed state.

[0050] The color indicator and its color change can be evaluated using appropriate optical and chemical sensors.

[0051] According to the aperture measurement method, the predominant phenolic body is made individually visible to the eye by developing a specific, clearly distinguishable color tone, and can thus be directly detected in a previously unknown, simple and quick way. Because the method used takes place within the ampoule, external influences are excluded. By refining the method with the aid of electronic optical sensors, data collection and storage is possible. This also results in an optimal, particularly easy-to-read gradation, as well as permanent storage of the resulting color tones. In this way, it is also possible for the first time to test fresh, untreated sample material and even fresh plants in pretreated or untreated form, even without prior drying, e.g.in the field, to be examined directly and analyzed for phenolic or cannabinoid components. This allows a comparison of the recorded data over the course of growth. The method according to the invention has proven particularly effective in the detection of cannabinoids. This makes it possible for the first time to distinguish between drug and industrial hemp in young or adult, male or female plants from the age of two weeks. Furthermore, the maturity of hemp plants can be determined quickly and easily, even directly on fresh plants. The areas of application of the method according to the invention include cannabis research and medicine, cannabis consumption (quality control) and official requirements for pharmacists, doctors, etc. Furthermore, the method can be used to detect cannabis preparations in biological sample materials.Through special modifications of the reagents and procedures, new methods for the selective detection of individual specific cannabinoids have been found.

[0052] Due to the simple design of the method and the required reagents, this procedure is particularly suitable for analysis kits that can be used directly on-site. Using software-supported, photometric measurement of the main absorption maxima of the developed colors, the method can also be used to quantitatively determine the detected phenolic substances. Data storage and comparison options enhance the safety of handling medical cannabis.

[0053] The solvents used in the reagent solution are preferably mono- or polyhydric alcohols, either alone or as a mixture. These ensure optimal extraction of the active ingredients and, on the other hand, good dissolving properties for the components of the dressing carton responsible for the bonding, thus ensuring a trouble-free color reaction. The components of the color dictator required for color development were designed so that they only need to be added to the reagent mixture in drop quantities for the simplest and most effective handling. The addition of organic acid salts to the reagent solution can improve the readability of the developed colors and extend their shelf life.

[0054] By storing the color tones accordingly, a database can be created that allows the hemp plants to be assigned according to location and origin. Comparative example: Examples of color representations:

[0055] Fresh hemp plants, immature drug hemp, violet reddish, mature blue-green, mature EU industrial hemp, violet reddish, thymol, deep blue, cannabidiol (CBD), violet pink, tetrahydrocannabinol, green-blue, cannabinol (CBN), blue Fresh, not dried, plant material is used to determine ripeness, as drying can alter the cannabinoid content (e.g., conversion of cannabidiol to THC). To distinguish industrial from drug hemp, fully developed, normal, finger-shaped leaves (fresh or dried) of the plant are used, not the flower or fruit clusters. By testing the normal, finger-shaped leaves of a specific young plant that is at least two weeks old, its maximum achievable future cannabinoid spectrum can be determined. If the developed colors are too intense and therefore difficult to read, the test should be repeated with a smaller sample size. Too small a sample size, on the other hand, can produce pale, indistinct, and yellow-shifted colors. In this case, the test should be repeated with a larger sample size. Conducting the tests:

[0056] A small amount of the sample is applied to a ring-shaped reagent-soaked surface 310 of a part of the Figure 3 The resulting color of the supernatant solution can be read within one minute. The best results are visible in transmitted daylight or against a bright surface. A very accurate result is naturally provided by a software-controlled evaluation using a photospectrometric device.

[0057] The features disclosed in the foregoing description and the appended claims may, separately or in combination, be subject to realization of the aspects of the disclosure made in the independent claims in different forms thereof.

Claims

1. A kit for the qualitative and / or quantitative detection of one or more substance(s) contained in the hemp plant, comprising: a) an ampoule (100) which is transparent and evacuated and at least partially consists of borosilicate glass; b) a material (120) comprising a hemp plant or parts thereof; and c) a color indicator adapted to react by contacting the hemp plant and / or at least a part thereof while changing the color of the color indicator; wherein the material (120) and the color indicator are disposed in the evacuated ampoule (100).

2. The kit of claim 1, wherein the material (120) comprises a cannabis blossom, marijuana, hashish, hashish oil, at least one cannabinoid, or a mixture thereof.

3. The kit of claim 1 or 2, wherein the color indicator comprises a color-forming substance, which is preferably selected from the group consisting of real black, real blue salt, dibromoquinone chlorimide, dichloroquinone chlorimide, vanillin, salicylaldehyde, formaldehyde, acetaldehyde, p-dimethylaminobenzaldehyde, diethylaminobenzaldehyde, ferric chloride, aminophenol, potassium hexacyanoferrate, and a mixture of two or more thereof.

4. The kit of any one of the preceding claims, wherein the color indicator comprises at least one solvent, which is preferably selected from the group consisting of water and mono- or polyhydric alcohols.

5. The kit of any one of claims 3 or 4, wherein the color indicator further comprises a reagent that assists the reaction of the material with the color-forming substance, wherein the reagent is preferably a basic compound, more preferably selected from the group consisting of alkali hydroxide, alkali carbonate, ammonium or alkali salts of an organic acid, and a mixture of two or more thereof.

6. The kit of any one of claims 3 to 5, wherein the color indicator further comprises a carrier material, preferably an absorbent neutral carrier material.

7. The kit of any one of the preceding claims, wherein the ampoule (100) is a separable ampoule (300) consisting of two or more parts (310, 320, 330, 340) that can be joined together to form the ampoule (300), wherein the color indicator is disposed on at least a part of an inner wall of one of the parts that can be joined together to form the ampoule (300).

8. The kit of claim 7, wherein the part on the inner wall of which the color indicator is at least partially disposed is a spacer which can be joined by screwing to one or more further parts (310, 320, 330, 340) of the separable ampoule (300).

9. A method for the qualitative and / or quantitative detection of one or more substance(s) contained in the hemp plant, comprising the steps of: a) providing a kit according to any one of claims 1 to 8; b) contacting the material (120) or parts thereof with the color indicator; and c) detecting a change in the color of the color indicator; characterized in that contacting the material (120) or parts thereof with the color indicator comprises heating the material (120) or parts thereof in the transparent, evacuated and at least partially borosilicate glass ampoule (100).

10. The method of claim 9, wherein the heating comprises inductive heating.

11. The method of any one of claims 9 or 10, wherein the detecting is performed using a color comparison scale, an optical sensor, a chemical sensor, or two or more thereof.

12. The method of any one of claims 9 to 11, further comprising a step, after the detecting, of matching information obtained by the detecting with data stored in a database.