COSMETIC PREPARATION AGAINST ACNE

Description

[0001] The present invention relates to a cosmetic product consisting of a packaging material with two spatially separated chambers, wherein the first chamber contains a lyophyllisate of bacteria of the strains C . acnes 6609 (H1), C1, C3, D1, A5, H1, H2, H3, K1, K2, K4, K6, K8, K9, L1 and / or F4, and the second chamber contains a water-containing cosmetic preparation in the form of an oil-in-water emulsion and a method for treating acne with this preparation.

[0002] The desire to look beautiful and attractive is rooted in human nature. Even though the ideal of beauty has changed over time, the pursuit of a flawless appearance has always been a human goal, as a pleasing appearance boosts self-esteem and attractiveness to others. The condition and appearance of the skin plays a significant role in a beautiful and attractive appearance.

[0003] In oily and impure skin, which is described as the transitional state between healthy, normal skin and pathologically altered acne-prone skin, the skin produces increased amounts of sebum (seborrhea). This sebum serves as a breeding ground for numerous microorganisms, especially Propionibacterium acnes (nowadays known as Cutibacterium acnes, C . acnes This explains why the new abbreviation was introduced in this revelation C . acnes instead of P . acnes is used) and PityrosporumThese species provide an ideal breeding ground. The microorganisms decompose the sebum into glycerol and fatty acids, which stimulates the sebaceous glands to increase production and attacks and destroys the follicle walls in the skin. This causes inflammation in the skin (pimples, pustules, nodules, cysts), which often heal only with scarring, thus permanently damaging the appearance of people suffering from impure skin (W. Umbach [ed.], Cosmetics, Development, Production and Application of Cosmetic Products, 2nd ed. Thieme Verlag, Stuttgart, 1995).

[0004] Acne (in the narrower sense) acne vulgaris Sebaceous cysts (SCCs) are various diseases of the sebaceous gland follicles, characterized by disorders of secretion and keratinization, subsequent inflammation, and possible scarring. acne vulgarisIt occurs mainly during puberty and usually concentrates on areas of skin rich in sebaceous glands (face, neck, chest, back). Sebaceous gland hyperplasia and a keratinization disorder of the follicles lead to their blockage with the formation of comedones and the associated symptoms. acne vulgaris typical efflorescences (Pschyrembel, Clinical Dictionary, 258th edition, Walter de Gruyter Publishing House, Berlin, 1998).

[0005] Conventional products for treating oily and / or impure skin, as well as acne-prone skin, usually have the disadvantage of stressing and drying out the skin and providing little nourishment.

[0006] Furthermore, the treatment of acne involves the use of strongly acidic (pH values ​​below pH 4.0) and strongly oxidizing agents (e.g., benzoyl peroxide), which strain the skin's acid mantle and attack or burn the skin.

[0007] US 2017 / 065647 A1 describes the treatment of acne by topical application of live strains of Cutibacterium acnes. WO 2017 / 136738 A2 describes three strains of Cutibacterium acnes , which are classified as K1 according to the SLST scheme and can be assigned to healthy skin.

[0008] It was therefore the object of the present invention to provide a significantly milder and better caring cosmetic and / or dermatological preparation for the treatment of oily and impure skin as well as for the treatment of acne (and in particular acne vulgaris ) to develop than they are known from the prior art. Furthermore, it was the object of the present invention to develop a cosmetic and / or dermatological preparation for the treatment of acne (and in particular acne vulgarisThe aim is to develop preparations that are effective at a skin-friendly pH (pH greater than 4.0), do not irritate the skin, and have minimal impact on the skin's protective acid mantle. Furthermore, preparations should be developed that suppress the growth and spread of acne-causing bacteria on the skin at a skin-friendly pH (pH greater than 4.0). These preparations should also significantly reduce the concentration of acne-causing bacteria on the skin and in the sebaceous glands.

[0009] The tasks are surprisingly solved by a cosmetic product consisting of a packaging material with two spatially separated chambers, the first chamber containing a lyophyllisate of bacteria from the strains C . acnes6609 (H1), C1, C3, D1, A5, H1, H2, H3, K1, K2, K4, K6, K8, K9, L1 and / or F4, and the second chamber contains a water-containing cosmetic preparation in the form of an oil-in-water emulsion, wherein the cosmetic preparation in the second chamber contains glyceryl stearate citrate, cetearyl alcohol, sodium cetearyl sulfate, glyceryl stearate, cetearyl sulfosuccinate, sodium stearoyl glutamate, polyglyceryl-3 methylglucose distearate, stearic acid, potassium cetyl phosphate, polyglyceryl-10 stearate (INCI polyglyceryl-10 stearate) and / or polyglyceryl-2 caprate.

[0010] The lyophyllisate can be obtained from bacteria of the strains C . acnes 6609 (H1), C1, C3, D1, A5, H1, H2, H3, K1, K2, K4, K6, K8, K9, L1 and / or F4 are prepared according to one of the conventional state-of-the-art methods for the lyophilization of bacteria (for example, with the addition of dextran and / or glycerin), which can be found in the standard works of pharmaceutical technology.

[0011] Although the prior art includes WO 2016 / 172196 and WO 2018 / 073651, which also cover the invention C . acnes Tribes reveal, but these writings could not point the way to the present invention because, among other things, it is not revealed with which medium the C. acnesBacteria can be activated in sufficient quantities. While WO 2016 / 172196 mentions the fundamental possibility of using freeze-dried bacteria, experts found it extremely difficult to reactivate them on the skin in medically safe yet effective quantities within a short time. Furthermore, it is not disclosed how the bacteria can be applied to the affected skin areas in a targeted and uniform (homogeneous) concentration in a way that is easy for laypersons to handle, as not every cosmetic product is a suitable carrier. Phase separation, agglomeration, separation from the preparation, and changes in viscosity are typical problems that occur when incorporating lyophilisates.Last but not least, virtually all cosmetics contain ingredients intended to protect them from microbial degradation, so that in a simple combination of cosmetic preparation and bacteria, it could be assumed that the latter would be more or less completely killed by the preservative components of the cosmetic preparation.

[0012] The first chamber may contain, in addition to the lyophyllisate from bacteria, cosmetic components such as oils and / or thickeners. It may also be advantageous according to the invention, as proposed in WO 2018 / 073651, to add peptone and / or hydroxyethylcellulose.

[0013] According to the invention, it is preferred that C . acnes Strains C3 and / or K8 are to be used. The combination of both strains in equal proportions is particularly preferred according to the invention.

[0014] According to the invention, it is particularly preferred to combine these strains (i.e., C3 and / or K8) with C. acnes To combine stems A5 and / or F4.

[0015] According to the invention, it is advantageous if the cosmetic preparation in the second chamber has a pH value of 4 to 8.

[0016] In order to give the product according to the invention the correct consistency for the application, it is advantageous according to the invention if the cosmetic preparation in the second chamber contains one or more thickening agents.

[0017] It is particularly advantageous within the meaning of the present invention if the cosmetic preparation in the second chamber has a viscosity of at least 500 mPas. According to the invention, the viscosities are measured using the Rheomat R 123 device with the spindle measuring body 1 and a constant rotational speed of 62.5 rpm at a temperature of 25°C.

[0018] According to the invention, it is preferred if the cosmetic preparation in the second chamber contains carrageenan and / or polyacrylic acid. According to the invention, the term polyacrylic acid also includes the salts (in particular the sodium salt) of this compound.

[0019] If the preparation contains carrageenan, a concentration of 0.01 to 1% by weight, based on the total weight of the cosmetic preparation in the second chamber, is preferred according to the invention.

[0020] If the preparation contains polyacrylic acid (carbomer), a concentration of 0.01 to 1% by weight, based on the total weight of the cosmetic preparation in the second chamber, is preferred according to the invention.

[0021] However, according to the invention, thickening the preparation in the second chamber with xanthan gum is a disadvantage because in this case agglomerate formation and clumping can occur in the preparation.

[0022] Therefore, advantageous embodiments of the present invention are characterized in that the cosmetic preparation in the second chamber is free of xanthan gum.

[0023] According to the invention, it is advantageous if the cosmetic preparation in the second chamber is free of parabens, methylisothiazolinone, chloromethylisothiazolinone, 3-iodo-2-propynyl butylcarbamate, DMDM ​​hydantoin, 2-hydroxy-4-methoxybenzophenone, 4-methoxycinnamic acid (2-ethylhexyl) ester, 4-methoxycinnamic acid isoamyl ester, and 3-(4-methylbenzylidene)camphor.

[0024] In general, it is preferred according to the invention if the cosmetic preparation in the second chamber is free of antimicrobial substances. Therefore, the second chamber of the product according to the invention is advantageously designed such that filling takes place under sterile conditions, the chamber is subsequently sealed airtight, and its contents are removed from the storage container by means of a dispensing device in a single dispensing (single application) and, after mixing with the lyophyllisate in the first chamber, are immediately ready for use.

[0025] Advantageous embodiments of the present invention are also characterized in that the cosmetic preparation in the second chamber contains glycerin, panthenol, ubiquinone Q10 and / or hyaluronic acid.

[0026] If the preparation contains glycerin, a concentration of 1 to 10% by weight, based on the total weight of the cosmetic preparation in the second chamber, is preferred according to the invention.

[0027] If the preparation contains panthenol, a concentration of 0.01 to 5% by weight, based on the total weight of the cosmetic preparation in the second chamber, is preferred according to the invention.

[0028] If the preparation contains hyaluronic acid, a concentration of 0.01 to 2 wt%, based on the total weight of the cosmetic preparation in the second chamber, is preferred according to the invention.

[0029] If the preparation contains ubiquinone Q10, a concentration of 0.01 to 0.05 wt%, based on the total weight of the cosmetic preparation in the second chamber, is preferred according to the invention.

[0030] Furthermore, according to the invention, the cosmetic preparation in the second chamber may preferably contain anti-inflammatory substances, for example polidocanol, bisabolol, aloe vera extract, chamomile flower extract, glycyrrhiza inflata root extract.

[0031] According to the invention, a water content of up to 100% by weight, based on the total weight of the cosmetic preparation in the second chamber, is advantageous for the cosmetic preparation in the second chamber.

[0032] According to the invention, it is advantageous if the cosmetic preparation in the second chamber contains salicylic acid, lactic acid and / or citric acid.

[0033] If the preparation contains salicylic acid, a concentration of 0.01 to 2% by weight, based on the total weight of the cosmetic preparation in the second chamber, is preferred according to the invention.

[0034] If the preparation contains lactic acid, a concentration of 0.01 to 3% by weight, based on the total weight of the cosmetic preparation in the second chamber, is preferred according to the invention.

[0035] If the preparation contains citric acid, a concentration of 0.01 to 2% by weight, based on the total weight of the cosmetic preparation in the second chamber, is preferred according to the invention.

[0036] According to the invention, the cosmetic preparation in the second chamber can advantageously contain one or more perfume substances. These perfume ingredients can be selected, for example, from the group of compounds including limonene, citral, linalool, alpha-isomethyl ionone, geraniol, citronellol, 2-isobutyl-4-hydroxy-4-methyltetrahydropyran, 2-tert-pentylcyclohexyl acetate, 3-methyl-5-phenyl-1-pentanol, 7-acetyl-1,1,3,4,4,6-hexamethyltetralin, adipic acid diesters, alpha-amylcinnamaldehyde, alpha-methyl ionone, amyl C, butylphenyl methylpropional cinnamal, amyl salicylate, amylcinnamyl alcohol, anise alcohol, benzoin, benzyl alcohol, benzyl benzoate, benzyl cinnamate, benzyl salicylate, bergamot oil, bitter orange oil, butylphenyl methylpropional, cardamom oil, cedrol, cinnamal, cinnamyl alcohol, and citronellyl methyl crotonate. Lemon Oil, Coumarin, Diethyl Succinate, Ethyllinalool, Eugenol, Ethylene Brassylate, Evernia Furfuracea Extract, Evernia Prunastri Extract, Farnesol, Guaiac Wood Oil, Geraniol, Hexyl Cinnamal, Hexyl Salicylate,Hydroxycitronellal, lavender oil, lemon oil, linalyl acetate, mandarin oil, menthyl PCA, methylheptenone, nutmeg oil, rosemary oil, sweet orange oil, terpineol, thymol, tonka bean oil, triethyl citrate and / or vanillin.

[0037] The cosmetic preparation in the second is in the form of an oil-in-water emulsion and contains glyceryl stearate citrate, cetearyl alcohol, sodium cetearyl sulfate, glyceryl stearate, cetearyl sulfosuccinate, sodium stearoyl glutamate, polyglyceryl-3 methylglucose distearate, stearic acid, potassium cetyl phosphate, polyglyceryl-10 stearate (INCI Polyglyceryl-10 Stearate) and / or polyglyceryl-2 caprate.

[0038] However, preparation methods based on W / O emulsions are disadvantageous according to the invention.

[0039] Advantageous embodiments of the present invention are also characterized in that the cosmetic preparation in the second chamber contains starch.

[0040] According to the invention, it is advantageous if the cosmetic preparation in the second chamber contains triglycerides and / or octyldodecanol in the oil phase.

[0041] In addition, the oil phase may contain oils from the group of lecithins, cocoglycerides, olive oil, sunflower oil, jojoba oil, soybean oil, peanut oil, rapeseed oil, almond oil, palm oil, coconut oil, castor oil, wheat germ oil, grapeseed oil, safflower oil, evening primrose oil, macadamia nut oil and the like.

[0042] However, it is disadvantageous if the cosmetic preparation in the second chamber contains silicone oils, mineral oil and polyethylene glycols.

[0043] Advantageous embodiments of the present invention are therefore characterized in that the cosmetic preparation in the second chamber is free of silicone oils, mineral oil and polyethylene glycols.

[0044] To ensure safe application from a medical point of view and effective application from a cosmetic point of view, a balanced ratio of lyophilisate to water-containing cosmetic preparation is important.

[0045] It is therefore advantageous according to the invention if the weight ratio of lyophilisate to water-containing cosmetic preparation is 1:10 to 1:100.

[0046] According to the invention, preferably the cosmetic product contains, after mixing the contents of both chambers, 1-10 wt% lyophilisate and 99-90 wt% of the water-containing cosmetic preparation.

[0047] Furthermore, it is advantageous in the sense of the present invention if the ratio of lyophilisate to water-containing cosmetic preparation is selected such that there are 10⁴ to 10⁷ bacteria per 2g of water-containing cosmetic preparation.

[0048] Advantageous embodiments of the present invention are characterized in that the packaging means includes a dispensing device and a mixing device with which the contents of both chambers can be mixed in the closed system and the mixture can be removed from an opening.

[0049] It is also advantageous according to the invention if the volume of the second chamber is 1.5 to 2.5 cm³.

[0050] Packaging materials may include, for example, Lyo-Ject®< dual-chamber syringes, V-LK®< dual-chamber cartridges or dual-chamber systems as disclosed in WO2018077598 A1.

[0051] According to the invention, a cosmetic product for use in a method for treating acne is also characterized in that the contents of the first and second chambers of a cosmetic product according to the invention, described above, are mixed and withdrawn and applied to the skin affected by acne in an amount of 0.003 to 0.005 g / cm².

[0052] The application to the skin usually takes place 0-12 seconds after the contents of both chambers have been mixed together.

[0053] In this process, it is advantageous according to the invention if the skin affected by acne is cleaned with a surfactant-containing cleansing preparation before the application of the cosmetic product.

[0054] It is also advantageous according to the invention if the skin affected by acne is disinfected with a benzoyl peroxide-containing preparation before applying the cosmetic product. Example

[0055] The following example, which is not according to the invention, is intended to illustrate the described items. Unless otherwise stated, all quantities, proportions, and percentages are based on the weight and total quantity or total weight of the preparations.

[0056] The following reference formulations can be supplied in packaging such as Lyo-Ject® dual-chamber syringes or multi-chamber blisters (Rohrer AG). The examples from the first and second chambers are combined below. first chamber [weight %] ingredient Example 1 Example 2 Example 3 Example 4 Example 5 Carrageenan 0,50 Citrate 0,17 C. acnes* 5,00 1,00 10,00 8,00 2,00 Glycerin 10,00 Sodium chloride 0,90 0,90 Neutral oil 20,00 Octyldodecanol 10,00 Polyacrylic acid 0,50 Strength 2,00 citric acid 0,09 second chamber [weight %] without preservative Carrageenan 0,20 Citrate 0,17 Glycerin 5,00 Hyaluronic acid 1,00 Polyacrylic acid 0,20 Water 94,24 92,34 58,10 91,80 85,80 citric acid 0,09 * The C. mentioned in claim 1 are used. acnes Strains used individually or in combination in the specified quantity.

Claims

1. Cosmetic product comprising a packaging means with two spatially separated chambers, wherein the first chamber contains a lyophilisate of bacteria of the strains P. acnes 6609 (H1), C1, C3, D1, A5, H1, H2, H3, K1, K2, K4, K6, K8, K9, L1 and / or F4, and the second chamber contains a water-containing cosmetic preparation in the form of an oil-in-water emulsion, characterized in that the cosmetic preparation in the second chamber contains glyceryl stearate citrate, cetearyl alcohol, sodium cetearyl sulphate, glyceryl stearate, cetearyl sulfosuccinate, sodium stearoyl glutamate, polyglyceryl-3-methylglucose distearate, stearic acid, potassium cetyl phosphate, polyglyceryl-10 stearate (INCI polyglyceryl-10 stearate) and / or polyglyceryl-2 caprate.

2. Cosmetic product according to claim 1, characterized in that the cosmetic preparation in the second chamber (a) has a pH value of 4 to 8, (b) contains carrageenan and / or polyacrylic acid, (c) is free from xanthan gum, (d) is free from parabens, methylisothiazolinone, methylchloroisothiazolinone, 3-iodo-2-propynyl butylcarbamate, DMDM hydantoin, 2-hydroxy-4-methoxybenzophenone, 4-methoxycinnamic acid (2-ethylhexyl) ester, 4-methoxycinnamic acid isoamyl ester, 3-(4-methylbenzylidene)camphor, and / or (e) contains glycerine, panthenol, ubiquinone Q10 and / or hyaluronic acid.

3. Cosmetic product according to one of the preceding claims, characterized in that the cosmetic preparation in the second chamber contains salicylic acid, lactic acid and / or citric acid.

4. Cosmetic product according to one of the preceding claims, characterized in that the cosmetic preparation in the second chamber contains triglycerides and / or octyldodecanol.

5. Cosmetic product according to one of the preceding claims, characterized in that the cosmetic preparation in the second chamber (a) contains one or more perfumes, (b) is free from silicone oils, mineral oil and polyethylene glycols, and / or (c) has a viscosity of at least 500 mPas.

6. Cosmetic product according to one of the preceding claims, characterized in that the ratio of lyophilisate to water-containing cosmetic preparation is selected such that there are 104 to 107 bacteria per 2 g of water-containing cosmetic preparation.

7. Cosmetic product according to one of the preceding claims, characterized in that a lyophilisate of bacteria of C. acnes strains C3 and / or K8 is used in the first chamber.

8. Cosmetic product according to claim 7, wherein the C. acnes strains C3 and K8 are used in equal proportions.

9. Cosmetic product according to claim 8 or 9, characterized in that the bacterial strains in the first chamber are combined with C. acnes strains A5 and / or F4.

10. Cosmetic product according to one of the preceding claims, characterized in that (a) the packaging means contains a dispensing device and a mixing device with which the contents of both chambers can be mixed in a closed system and the mixture can be dispensed from an opening, and / or (b) the volume of the second chamber is 1.5 to 2.5 cm3.

11. Cosmetic product according to any of claims 1-10 for use in a method for treating acne, characterized in that the contents of the first and second chambers of the cosmetic product are mixed, dispensed and applied in an amount of 0.003 to 0.005 g / cm2 to the skin affected by acne.

12. Cosmetic product for use in a method according to claim 11, characterized in that the skin affected by acne is cleansed with a surfactant-containing cleansing preparation before the cosmetic product is applied.

13. Cosmetic product for use in a method according to one of claims 11 or 12, characterized in that the skin affected by acne is disinfected with a benzoyl peroxide-containing preparation before applying the cosmetic product.