Cosmetic composition combining water-soluble extracts of delipidated tubers with lipid extracts of Cyperus esculentus.

Abstract

Cosmetic use of combinations of water-soluble extracts of delipidated tubers with lipid extracts of Cyperus esculentus of the Cyperaceae family intended to be placed in contact with the various superficial parts of the human body in order to slow down the growth or regrowth of hair.

Description

Title of the invention: Cosmetic composition combining water-soluble extracts of delipidated tubers with lipid extracts of Cyperus esculentus.

[0001] The present invention relates to a cosmetic composition intended to be placed in contact with the various superficial parts of the human body (epidermis, hair and scalp systems, nails, lips and external genital organs) or with the teeth and oral mucosa, with a view, exclusively or mainly, to cleaning them, perfuming them, changing their appearance, protecting them, keeping them in good condition or correcting body odors and characterized in that it comprises at least one aqueous, glycerinated or hydroethanolic extract of delipidated tubers associated with a lipid extract both from Cyperus esculentus of the Cyperaceae family with the aim of slowing down the growth or regrowth of hair and to be used directly on the skin or after hair removal carried out manually or with laser-type devices or pulsed polychromatic lamps (PPL).

[0002] We know that more and more cosmetics are trying to claim a more organic, more ecological, more natural mode of action with the new ISO 16128 standard, vegan and even a slow cosmetics (ecological, healthy, intelligent and reasonable) taking into account the real needs of the skin, advocating the use of fewer ingredients (skinimalism) to do better and realistic promises. One possible response to this concern could be plant extracts from the same plant in order to achieve a complete effect.

[0003] Many people believe that the skin surface to which cosmetics are applied is a dead and inert structure. In fact, this is not the case, and the epidermis, including its outermost stratum corneum, is the site of intense biochemical reactions where activators, inhibitors, and inhibitors of inhibitors maintain a balance and homeostasis that varies with the time of day, the seasons, and age. Each epidermal cell, and particularly the keratinocytes, which are the predominant cells of the epidermis, exhibits a complex and intricate metabolism leading to cell migration, maturation, and differentiation under the control of the physiological expression of the 36,000 genes and variants that comprise them.

[0004] It is then easy to imagine the interest of being able to select and test plant substances on the expression of all these genes in order to be able to select the most interesting extracts and develop the most efficient extraction and production methods.

[0005] Among all these genes, there are those designated WNT for WiNgless-Type, with wingless for wingless, which have several functions, including those of interest to us here, namely that in case of activation it causes the formation of additional hairs but that in case of blockage the skin then becomes hairless.

[0006] When the plantar skin of the foot is tested, the presence of a highly expressed protein (DKK2) is observed, naturally inhibiting WNTs genes as described in the publication: Song et al., Regional Control of Hairless versus Hair-Bearing Skin by Dkk2, Cell Reports (2018), https: / / doi.Org / 10.1016 / j.celrep.2018.ll.017. This explains why humans do not have hair on the soles of their hands and feet.

[0007] We understand the interest in finding a plant, if possible edible, and extracts usable in cosmetics capable of slowing down the production of WNTs by the skin, in the manner of DKK2 naturally produced under our hands and feet, in order to act on the growth and regrowth of hair.

[0008] The applicant has indeed tested several aqueous, glycerinated, or hydroethanolic extracts of delipidated Cyperus esculentus tubers on the complete genome of normal human keratinocytes for this purpose, and particularly by analyzing genome-wide gene expression (36,000 transcribed genes and variants) of normal human epidermal keratinocytes following the extraction of total transcribed Ribonucleic Acids (RNA) and subsequent hybridization on Deoxyribonucleic Acid (DNA) microarrays. The principle of the DNA microarray is based on the property of denatured DNA (single strand) to spontaneously reform its double helix when in the presence of a complementary strand (hybridization reaction).Since it is possible to attach up to a million probes to a biochip, DNA chips constitute a massive approach that the inventor used because they allow in a single experiment to have an estimate on the expression of tens of thousands of genes, including the expression of those capable of slowing down or blocking the production of WNTs produced by the skin.

[0009] Moreover, Cyperus esculentus, from the Cyperaceae family, is used primarily for its oil, which is obtained by pressing the tubers of this rhizomatous plant. It is an edible plant whose tubers are harvested to obtain, among other things, a valuable oil. The Arabs and Egyptians already used it for cosmetic purposes due to its nourishing, revitalizing, and antioxidant effects, and also in food to give it an almond-like flavor. It is also used for a variety of internal and external treatments, such as the prevention of cardiovascular disorders, the reduction of post-meal glucose levels, the increase in mitochondrial formation, and the prevention of cognitive impairment.

[0010] It was based on the observation that plants, and particularly their tubers, very often contain a significant amount of lipids that hinder the release of substances of interest and necessitate the use of complex solvents other than simple water, hydroethanolic solvents, or vegetable glycerin. From this observation arose the idea of ​​first separating these lipids from the rest of the plant to be extracted, with the possibility of combining these extracts with the lipids if necessary. This delipidation of the tubers has the effect of releasing water-soluble components for extraction that would otherwise be insoluble.

[0011] It was by studying the properties of different water-soluble extracts of Cyperus esculentus tubers, after delipidation obtained after one or more successive cold pressings, associated with lipid extracts of the same plant, on the expression of genes of normal human keratinocytes that the applicant discovered quite unexpectedly that these extracts of Cyperus esculentus slowed down the production of these WNTs.

[0012] One of the aims of the invention is to provide a complete composition for topical use using the lipid part of the plant associated with its water-soluble part which can be extracted successively and therefore easily for achievements that are entirely sustainable development and slow cosmetics.

[0013] In the context of the invention, a water-soluble extract of a plant or part of a plant, such as tubers, is understood to be the result of any extraction in a water-soluble solvent, such as water itself, a hydroethanolic solution, or glycerin, and which can serve as a cosmetically acceptable vehicle and therefore be directly formulatable to produce a final cosmetic composition. These extracts can be formulated directly at concentrations of approximately 0.1% to 5%, or stored in a dry state after evaporation of the water or water plus ethanol, or even as is in the case of a glycerin solvent.

[0014] One of the aims of the invention is therefore to provide a complete composition for topical use using the lipid part of the plant associated with its water-soluble part which can be extracted successively and therefore easily.

[0015] Another object of the invention is to propose the use of such a composition in the context of a cosmetic application.

[0016] The invention therefore relates to a cosmetic composition comprising an association of a lipid extract with a water-soluble extract both derived from tubers of Cyperus esculentus of the Cyperaceae family and a cosmetically acceptable vehicle.

[0017] Advantageously, said cosmetically acceptable vehicle is also the one used as a solvent during the extraction of the water-soluble extract and is chosen among water with or without enzyme, a hydroethanolic solution or a water / glycerol mixture.

[0018] The invention also relates to a composition characterized in that said water-soluble extract of delipidated tubers of Cyperus esculentus of the Cyperaceae family does not include any lipids and that said lipid extract of the same plant is obtained by cold pressing.

[0019] The invention further relates to a cosmetic composition characterized in that said water-soluble extract of delipidated tubers of Cyperus esculentus of the Cyperaceae family is made from tubers previously delipidated by cold pressing, one to three times successively, in order to obtain a content of 10% or less by mass of lipids relative to the total mass of the tubers.

[0020] The invention advantageously also relates to a composition characterized in that said extract of delipidated tubers of Cyperus esculentus of the Cyperaceae family has been made water-soluble by solubilization in a solvent chosen from water with or without enzyme, a hydroethanolic solution or a water / glycerol mixture before being able to be used as is in the composition or stored after complete drying before being resolubilized in these same cosmetically acceptable solvents before use.

[0021] Even more advantageously, said association of a lipid extract with a water-soluble extract of Cyperus esculentus tubers from the Cyperaceae family is intended to produce cosmetic compositions that slow down the growth and regrowth of hair and is to be used directly on the skin or after hair removal carried out manually or with laser-type devices or pulsed polychromatic lamps (PPL).

[0022] The invention also relates to a composition which is in the form of an oil-in-water (O / W) or water-in-oil (W / O) emulsion, multiple emulsion (O / W / O, W / O / O), microemulsion, twin-phase emulsion, PIT emulsion, nanoemulsion, pseudoemulsion, aqueous gel, oily gel, hydroalcoholic gel, suspension, lyophilized gel or emulsion and / or one of the preceding forms containing microcapsules, nanocapsules, liposomes, ethosomes and which may be in the form of creams, hydrolipidic serums, liquids, pastes, lotions, emulsions, gels, solids, powders, masks, sticks, sprays, aerosols.

[0023] The invention further relates to a composition being in the form of a two-phase micellar emulsion, a foaming hydrolipidic gel, a jelly lotion, an exfoliant, a moisturizing cream, a moisturizing serum, a moisturizing body lotion, an anti-regrowth mask, an anti-regrowth cream, a body cream, a cream for intimate areas, a regenerating anti-regrowth serum, a balm, a mist, a concentrate.

[0024] And so, the non-therapeutic cosmetic use of a composition for topical application on healthy skin for the purpose of producing cosmetic compositions slowing down the growth and regrowth of hair to be used directly on the skin and scalp or after hair removal carried out manually or with laser-type devices or even pulsed polychromatic lamps (PPL) in the manner of the physiological functioning of the skin of the palms of the hands or the soles of the feet which are normally hairless.

[0025] And also a composition for its use for the treatment or prevention of hair growth and regrowth to be used directly on the skin and scalp or after hair removal carried out manually or with laser-type devices or pulsed polychromatic lamps (PPL) by inhibiting by more than 50% the production of WNTs proteins essential for the development of hair follicles in the manner of the physiological phenomena taking place at the level of the palms of the hands and the soles of the feet which are normally hairless.

[0026] The invention therefore relates to a cosmetic or pharmaceutical composition made from an extract of delipidated tubers of African Cyperus esculentus of the Cyperaceae family, characterized in that it is intended to be applied to healthy skin in order to slow down the growth and regrowth of hair, to be used directly on the skin and scalp.

[0027] Therefore, a cosmetic or pharmaceutical composition intended to be placed in contact with the various superficial parts of the human body (epidermis, hair and scalp systems, nails, lips and external genital organs) or with the teeth and oral mucosa, with a view exclusively or mainly to cleaning them, perfuming them, changing their appearance, protecting them, keeping them in good condition or correcting body odors and characterized in that it comprises at least one water-soluble extract of delipidated tubers of Cyperus esculentus of the Cyperaceae family with the aim of slowing down the growth and regrowth of hair to be used directly on the skin and scalp for bald people with unsightly residual hair.

[0028] Even more advantageously, said water-soluble extract of delipidated tubers of Cyperus esculentus of the Cyperaceae family comprises 0% by mass of lipids relative to the total mass of the extract.

[0029] Also within the scope of the invention, said extract of delipidated tubers of Cyperus esculentus of the Cyperaceae family has been made water-soluble by solubilization in a solvent chosen from water with or without enzyme, a hydroethanolic solution or a water / glycerol mixture before being able to be used as is in the composition or stored after complete drying before being resolubilized in these same cosmetically acceptable solvents before use.

[0030] The effects of different plant extracts on gene expression in Normal Human Epidermal Keratinocytes (NHEK) were investigated. More specifically, a full transcriptome analysis, i.e., the analysis of all genes expressed at a given time, was performed using the Affymetrix GeneAtlas platform and the U219 "full human transcriptome" chip containing 36,000 so-called transcribed genes and variants.

[0031] This method, described in more detail in the examples below, allows us to choose, based on the genes stimulated, i.e. whose expression is significantly increased, or inhibited, i.e. whose expression is significantly reduced, the most interesting plants for cosmetic use and which has shown the interest of Cyperus esculentus of the Cyperaceae family among other plants thus tested.

[0032] Then, by taking the most interesting gene or genes, this method makes it possible to select the optimum protocol(s) in order to obtain the best possible results. Here, for Cyperus esculentus of the Cyperaceae family, the genes encoding the WNTs proteins (WNT4 and WNT10A), essential for the development of hair follicles, were used to determine that it was the delipidated tubers, subsequently extracted in a simple solvent, that made it possible to obtain a reduction in expression of more than 50% upon contact with normal human keratinocytes (NHEK). The relative expression of this WNT4 gene in control keratinocytes had a signal intensity of 99.43, while in keratinocytes brought into contact with the water-soluble extract of Cyperus esculentus thus selected had a signal intensity of 41.13, i.e., an inhibition, under experimental conditions, of the expression of this gene called fold change or rate of modification, of 0.41.

[0033] This made it possible to determine an optimum when the extraction was carried out in simple solvents such as water, hydroethanolic mixtures in which the ethanol is then recycled after complete evaporation or mixtures of water and glycerin, whose moisturizing properties for the skin are well established.

[0034] These tests demonstrate the value of a cosmetic composition characterized in that it consists of a water-soluble extract of defatted tubers of Cyperus esculentus from the Cyperaceae family. The tubers, separated from their rhizome, are then dried and defatted by one or more cold pressings, followed by oven drying for 1 to 5 hours at a temperature between 50°C and 60°C to prevent the proliferation of microorganisms. The extract is then extracted or macerated in water with or without added enzymes, a hydroethanolic solution, a water / glycerol mixture, or macerated in glycerol alone. It can then be filtered or centrifuged and stored and used directly in its respective solvent, or dried again, or freeze-dried. or even zeodrate, which, after topical application to healthy skin, has the effect of fighting against hair growth and regrowth.

[0035] The invention also relates to a water-soluble extract of delipidated tubers of Cyperus esculentus of the Cyperaceae family characterized in that it comprises water with or without enzyme, a hydroethanolic solution or a water / glycerol mixture and that it is prepared from 10% by weight of delipidated and then dried tubers.

[0036] This allows obtaining a water-soluble extract of delipidized tubers of Cyperus esculentus of the Cyperaceae family characterized in that it is intended to be applied to healthy skin to limit the growth and regrowth of hair.

[0037] Or more specifically, a composition comprising a water-soluble extract of delipidated tubers of Cyperus esculentus of the Cyperaceae family for its use in the treatment or prevention of the hair growth stimulation effects caused by over-expression of WNT4 and WNT10A for WiNgless-Type 4 and 10A causing the formation and growth of additional hairs.

[0038] A cosmetic skincare method for the advantageous use of plant extracts that allow both the slowing down and regrowth of hair and fur, comprising the topical administration of a cosmetic composition made from a water-soluble extract of delipidated tubers of Cyperus esculentus and a lipid extract from the same plant, optimized by the use of whole genome expression analysis of cultured human keratinocytes (NHEK) in order to determine all of its properties as well as the optimization of its preparations, the association of enzymes or not during the extraction or maceration steps, the interest or not of prior roasting, the % of delipidated and dried tubers or the nature of the final drying and characterized both by an increase in the expression of certain genes of interest and by a decrease in the expression of other genes of interest.

[0039] Examples are given below without being limiting in order to illustrate the invention.

[0040] Example 1. Obtaining water-soluble extracts from delipidated tubers of Cyperus esculentus. Extracts of defatted tubers of Cyperus esculentus, from the Cyperaceae family, are obtained by harvesting the tubers followed by initial drying. The tubers are then defatted by one or more cold pressings, followed by oven drying for 1 to 5 hours at a temperature between 50°C and 60°C to prevent the proliferation of microorganisms. The extract is then extracted or macerated in water with or without added enzymes, a hydroethanolic solution, a water / glycerol mixture, or macerated in glycerol alone. It can then be filtered or centrifuged and stored for use. directly in their respective solvent or again dried or freeze-dried or even zeodrated. They can therefore include water with or without enzyme, a hydroethanolic solution or a water / glycerol mixture and be prepared from 10% by weight of delipidated tubers then dried from the solvent used for extraction or maceration. These extracts can be tested directly or they can be dried, dehydrated, freeze-dried or zeodrated before testing. In the latter cases they are rehydrated or diluted with purified water acting as a solvent. We will call the water-soluble extracts obtained and used in the following examples ECE (for Extracts of Cyperus Esculentus).

[0041] Example 2. Tests and examples of results. The biological model: Third-pass normal human epidermal keratinocytes (NHEK) cultured at 37°C and 5% CO2. Culture medium supplemented with EGF at 0.25 ng / ml and EP at 25 µg plus gentamicin 25 µg. The assay medium consisted of SFM keratinocytes with only gentamicin 25 µg / ml. Experimental conditions: Keratinocytes were seeded in 24-well plates and cultured in culture medium for 24 hours, then in test medium for an additional 24 hours. The medium was then replaced with test medium containing or not (control) the compounds under investigation, and the cells were incubated for 24 hours. All experimental conditions were performed in triplicate (n=3). At the end of incubation, the culture supernatants were removed and the cell mats were rinsed with a PBS solution. The plate was immediately frozen dry at -80°C. Prior to extraction, the culture replicates were pooled. Total RNA from each sample was extracted using TriPure Isolation Reagent® according to the protocol recommended by the supplier. The quantity and quality of RNA were assessed by capillary electrophoresis (Bioanalyzer 2100, Agilent). The synthesis of biotinylated antisense RNAs (aRNAs) was carried out using the "GeneChip 3'IVT Express" kit (Affymetrix®). For each biotinylated RNA sample, an electrophoretic profile was performed (Bioanalyzer 2100, Agilent) before and after fragmentation. The hybridization of labeled and fragmented RNAs on the Affymetrix® U219 chip (36,000 transcripts and variants) was carried out on the GeneAtlas™ fluidics Affymetrix ® hybridization station for 20 hours at 45 °C. The U219 chips were then scanned using the GeneAtlas™ Imaging station (Affymetrix® - 2 pm resolution) to generate signal intensity data as shown in the result examples below. Signal intensity data were normalized using Expression Console software (Affymetrix®), based on the RMA algorithm. Quality control of the labeling and hybridization was then performed. Quality controls of the hybridization and labeling steps validated the experimental process. Once normalized with Expression Console software, the data were transferred and formatted in a Microsoft Excel file. Calculations and filters have been added to these files to sort the data and facilitate its use. The "fold change" thresholds or rate of change (value corresponding to the ratio: signal intensity value of a probe corresponding to the treated sample / signal intensity value of a probe corresponding to the control) were defined and applied to the normalized data. The following abbreviations are used in the analysis files: - UR: Upregulated - overexpression or stimulation of the probe or gene when the fold change is greater than 2, - DR: Downregulated - underexpression or inhibition of the probe or gene when the fold change is less than 0.5. Each analysis file consists of: - The signal intensity value (RE: Relative Expression) corresponding to each of the samples, - Calculations of "fold change" or rate of change for each comparison, - Information relating to each gene. The filters inserted into these files allow the selection of significantly modulated genes. The example of gene probes corresponding to the variation in expression of WNT 4 and WNT 10A obtained with this methodology and with ECE for the Cyperus Esculentus delipidated tuber extract, the numbers indicated correspond to the signal intensity obtained for the control and for the ECE assay: Intensity of Winglet-type 4 (WNT4) signals: - Control: 99.43 - ECE: 41.13 - Change rate: 0.41 - Regulation: DR Winglet-type 10A (WNT 1 OA) signal intensity: - Witness: 153.78 - ECE: 74.09 - Change rate: 0.48 - Regulation: DR

[0042] In the examples below, which give examples of the realization of these compositions, without this list being exhaustive, the following abbreviations will be used: ECE for Extract of delipidated tubers of Cyperus Esculentus and Cyperus Oil for Cyperus esculentus Oil.

[0043] Example 3. According to a first embodiment of the invention, the composition comprises: Carboxypolymethylene or carboxylic polymer..................0.40 g Diethanolamine cetylphosphate............................................1.50g Octyl dodecanol.............................................................5.50 g Cetyl alcohol..............................................................1.00 g Mineral oil............................................................2.00 g Stearic acid.............................................................1.00 g Sterylic alcohol ethoxylate (20 E).........................................0.30 g Ketostearyl alcohol....................................................1.80 g Preservative...................................................................0.15 g Cyperus oil.............................................................0.50 g Triethanolamine........................................................0.60 g Cyclomethicone.............................................................12.00 g Dimethicone copolyol........................................................3.00 g Phenyl trimeticone...........................................................2.00 g ECE................................................................................3.00 g Dextran........................................................................1.00 g Glycerin.........................................................................2.00 g Demineralized water qsp.........................................................100 g After 4 weeks of combined use of this composition and a PPL-type hair removal device on one of the forearms of 50 volunteers, the skin appears with less hair and significantly shorter hair compared to the control forearm which only underwent PPL hair removal.

[0044] Example 4. According to a second embodiment of the invention, the composition comprises - Acrylate C10-30........................................................0.50 g - Arginine............................................................0.001g - Glycerin..................................................................................1.00 g - ECE............................................................................................0.20 g - Alcohol..................................................................2.00 g - Carbomer................................................................................0.126 g - EDTA..............................................................................................0.005 g - Preservative......................................................................0.867 g - Cyperus oil..................................................................................0.5 g - Hydrogenated lecithin.....................................................0.09 g - Hyaluronic acid....................................................................0.20 g - TEA 99%...................................................................................0.62 g - Colorings........................................................................0.0002 g - Demineralized water qsp.................................................................100 g After 4 weeks of use on 50 volunteers aged 20 to 70, the skin appeared significantly with less hair and shorter hairs.

[0045] Example 5. Oil-in-water gel emulsion - Carbopol 981 .........................................................0.6 g - Ethyl alcohol...........................................................................15 g - Volatile silicone oil...............................................................3 g - Cyperus oil.............................................................................7 g - Preservative...............................................................0.3 g - Fragrance.....................................................................0.4 g - ECE..................................................................................0.1 g - Triethanolamine.........................................................................0.2 g - Demineralized water qsp...................................................................100 g

Claims

Demands

1. Cosmetic composition comprising a combination of a lipid extract obtained by cold pressing, a water-soluble extract of delipidated tubers containing no lipids, both from tubers of Cyperus esculentus of the family Cyperaceae, and a cosmetically acceptable vehicle.

2. Cosmetic composition according to claim 1, wherein said cosmetically acceptable vehicle is also that used as solvent during the extraction of the water-soluble extract and is selected from water with or without enzyme, a hydroethanolic solution or a water / glycerol mixture.

3. Cosmetic composition according to any one of claims 1 to 2 characterized in that said water-soluble extract of delipidated tubers of Cyperus esculentus of the Cyperaceae family does not comprise any lipids and that said lipid extract of the same plant is obtained by cold pressing.

4. Cosmetic composition according to any one of claims 1 to 3 characterized in that said water-soluble extract of delipidated tubers of Cyperus esculentus of the family Cyperaceae is made from tubers previously delipidated by cold pressing, one to three times successively, in order to obtain a content of 10% or less by mass of lipids relative to the total mass of the tubers.

5. Cosmetic composition according to any one of claims 1 to 4 characterized in that said extract of delipidated tubers of Cyperus esculentus of the family Cyperaceae has been made water-soluble by solubilization in a solvent selected from water with or without enzyme, a hydroethanolic solution or a water / glycerol mixture before being able to be used as is in the composition or stored after complete drying before being resolubilized in these same cosmetically acceptable solvents before use.

6. Composition according to any one of claims 1 to 5 characterized in that said combination of a lipid extract with a water-soluble extract of Cyperus esculentus tubers of the Cyperaceae family slows down hair growth and regrowth and is for use directly on the skin or after hair removal manually or with laser-type devices or pulsed polychromatic lamps (PPL).

7. Composition according to any one of claims 1 to 6, which is in the form of an oil-in-water (O / W) or water-in-oil (W / O) emulsion, multiple emulsion (O / W / O, W / O / O), microemulsion, twin-phase emulsion, PIT emulsion, nanoemulsion, pseudoemulsion, aqueous gel, oily gel, hydroalcoholic gel, suspension, lyophilized gel or emulsion and / or any of the preceding forms containing microcapsules, nanocapsules, liposomes, ethosomes and may be in the form of creams, hydrolipidic serums, liquids, pastes, lotions, emulsions, gels, solids, powders, masks, sticks, sprays, aerosols.

8. Composition according to any one of claims 1 to 6, being in the form of a two-phase micellar emulsion, a foaming hydrolipidic gel, a jelly lotion, an exfoliant, a moisturizing cream, a moisturizing serum, a moisturizing body lotion, an anti-regrowth mask, an anti-regrowth cream, a body cream, an intimate area cream, an anti-regrowth regenerating serum, a balm, a mist, a concentrate.

9. Non-therapeutic cosmetic use of a composition as defined in any one of claims 1 to 8, for topical application to healthy skin for the purpose of slowing down hair growth and regrowth, to be used directly on the skin and scalp or after hair removal carried out manually or with laser-type devices or pulsed polychromatic lamps (PPL) in the manner of the physiological functioning of the skin of the palms of the hands or the soles of the feet which are normally hairless.

10. Composition according to any one of claims 1 to 8 for its use for the treatment or prevention of hair growth and regrowth for use directly on the skin and scalp or after hair removal carried out manually or with laser-type devices or pulsed polychromatic lamps (PPL) by inhibiting by more than 50% the production of WNTs proteins essential for the development of hair follicles in the manner of the physiological phenomena taking place on the palms of the hands and soles of the feet which are normally hairless.