Functional material derived from natural substances and use thereof

Abstract

[Problem] The purpose of the present invention is to pay attention to so-called upgrading and remanufacturing when selecting a material derived from a natural substance, and to provide a material having an anti-glycation inhibitory effect (hereinafter referred to as "AGE production inhibitory effect") against a glycation reaction of a protein in vivo. The composition can be used for external preparations (such as cosmetics) requiring consideration of skin affinity and food and beverage components requiring consideration of nutrition. [Solution] The present invention solves this problem by irradiating white auricularia polytricha produced by mutation of Auricularia polytricha (Mont.) Sacc. With an ultraviolet B wave and treating the obtained product under prescribed conditions, thereby obtaining an active ingredient having an ideal function and rich in vitamin D2 and amino acids.

Description

Technical Field

[0001] This invention relates to a functional material discovered based on the specificity of white hairy fungus (Auricularia polytricha) and its uses, which is produced by a mutation of hairy fungus (Auricularia polytricha (Mont.) Sacc.).

[0002] More specifically, in selecting materials derived from natural products, this invention emphasizes so-called upgraded regeneration, which has anti-glycation effects against the glycation reactions of proteins in the body (hereinafter referred to as "AGE generation inhibition"), and relates to its use in topical formulations (such as cosmetics) considering skin affinity and in food and beverage products considering nutrition. Background Technology

[0003] Since ancient times, humankind has desired to "remain young forever," leading to the development of so-called anti-aging or "anti-aging drugs," and a medical system to overcome the aging process and its causes.

[0004] In recent years, glycation has received much attention as one of the risk factors for aging. Glycation refers to the non-enzymatic chemical reaction between carbohydrates and proteins (amino acids), and the substances produced by this chemical reaction are collectively referred to as AGEs (advanced glycation end products).

[0005] Protein glycation has been reported to occur even in healthy individuals; however, recent findings indicate that ultraviolet radiation and aging accelerate glycation, leading to the production and accumulation of advanced glycation end products (AGEs). Diseases associated with recent lifestyle and dietary changes are also significant risk factors. For example, individuals with diabetes are at particularly high risk and are more prone to AGE accumulation, thus accelerating various aging phenomena.

[0006] Because the accumulation of AGEs is accompanied by the hardening and denaturation of proteins, its progression can lead to a decline in the function of body tissues, raising concerns about various health effects.

[0007] Furthermore, since proteins exist in body tissues such as the brain, organs, skin, scalp, hair, nails, and blood, the effects of AGE production and accumulation naturally extend throughout the body.

[0008] Collagen is a protein that makes up about 30% of body weight, with 40% found in the skin (including eyelid skin) and 10% to 20% in bones and cartilage. In addition, collagen is also found in blood vessels, scalp, and organs, and glycation occurs in each of these areas.

[0009] Like collagen, elastin is an important protein in the human body. It is a fibrous component that provides elasticity by linking collagen together in a network structure, and plays a role in giving skin its elasticity.

[0010] Therefore, both collagen and elastin are directly affected by AGEs. The specific effects of AGEs, especially on the skin, blood vessels, and scalp, are as follows: In other words, in the skin (including the skin around the eyes; the same term is sometimes used interchangeably below), proteins such as collagen and elastin fibers undergo glycation, triggering a biological reaction that destroys the collagen fiber structure that maintains skin firmness. Enzymes that inhibit collagen synthesis (collagenases) are also activated. As a result, the skin loses its normal elasticity, becomes lighter in color, lacks firmness and radiance, and metabolic waste accumulates in skin cells. This ultimately leads to so-called dullness and darkening of the skin, causing it to lose its translucency.

[0011] In addition, rough skin during dieting is usually caused by nutritional imbalance. However, if an unreasonable diet continues, it will not only damage the stratum corneum, which has a barrier function and lose its moisturizing ability, but also accelerate the decline in skin elasticity due to glycation.

[0012] Dark circles become more noticeable in the eyelid area, and eyelashes lose elasticity, firmness, and shine.

[0013] Advanced AGEs in the blood have a high affinity and adhesion to vascular cells, easily accumulating on the blood vessel walls. When AGEs adhere to the blood vessel walls, they produce reactive oxygen species (ROS), which oxidize nitric oxide (NO), leading to impaired blood flow and thus triggering lipid metabolism disorders.

[0014] Like the skin, the scalp also has a dermis. When AGEs cross-link in the scalp dermis, or when keratin (a protein that makes up the main component of hair) undergoes glycosylation and leads to the accumulation of AGEs, hair loss, breakage, and graying are exacerbated.

[0015] In addition, substances such as elastin, hyaluronic acid, and chondroitin sulfate present in the dermis contribute to the firmness of the scalp.

[0016] The effects on hair are also significant. When glycation of collagen and elastin continues in the dermis, the dermis hardens, adversely affecting glands, hair follicles, and blood vessels. As a result, hair growth vitality decreases, negatively impacting hair quality, such as loss of firmness and shine. Hair growth is regulated by the dermal papilla at the hair root. The dermal papilla stimulates hair matrix cells, which then divide and keratinize to form hair, extending outwards from the scalp. When AGEs accumulate at the hair root, the function of the dermal papilla and hair matrix cells is restricted, preventing sufficient hair growth and leading to problems like hair loss. Eyelashes can be considered body hair growing along the eyelid margin, but similar to hair, glycation in the eyelid skin degrades proteins such as collagen and elastin, causing eyelashes to lose elasticity, firmness, and shine, and reducing the regeneration cycle.

[0017] In addition, melanocytes are adjacent to hair matrix cells. When hair matrix cells divide, they absorb melanin from melanocytes, which makes hair black or brown. However, if AGEs accumulate in melanocytes, they cannot provide enough melanin, which can also lead to gray hair.

[0018] Therefore, from the perspective of scalp, hair and eyelash care, anti-glycation (glycation countermeasures), that is, measures to inhibit glycation, is receiving widespread attention.

[0019] Regarding collagen, in youth, collagen-synthesizing enzymes are actively secreted, resulting in abundant collagen production and maintaining the normal condition of the skin, blood vessels, and scalp. However, with age, collagen-synthesizing enzymes decrease, leading to the continuous production of AGEs (Advanced Glycation End Products). When this phase continues, localized care, such as so-called UV protection and moisturizing measures, is no longer sufficient, requiring systemic care including diet. The situation is similar for elastin.

[0020] Existing technologies have been studied from the perspective of actively ingesting collagen and promoting its synthesis. For example, Patent Document 1 discloses that by ingesting large amounts of collagen or its hydrolysates, the biosynthesis of biological collagen can be promoted, and various diseases associated with decreased biological collagen metabolism can be improved. Patent Document 2 discloses a skin activator that uses Eucommia ulmoides or its extract and ginseng or its extract as main components, and selectively mixes them with deoxyribonucleic acid, chondroitin sulfate, collagen or its hydrolysates, and coix seed extract, showing an effect of improving skin renewal. Patent Document 3 discloses an oral hair growth agent that is mixed with kelp root, hydrangea, ginseng, and collagen, which can promote the function of hair follicle cells and has a hair growth effect. Patent Document 4 discloses a collagen synthesis promoter composition that utilizes specific plants to enhance the ability of organisms to promote collagen synthesis, thereby restoring the metabolism of biological tissues.

[0021] However, although these patent documents focus on application materials derived from natural products, they are not fundamental countermeasures against saccharification reactions like this invention, and the effects achieved are not sufficient.

[0022] For example, patent documents 5 to 7 can be cited as examples of patent applications for inhibitors of AGE production. Patent documents 5 and 7 disclose the application of the drug. In addition, patent document 6 discloses a method for solving skin quality improvement problems (such as wrinkles, age spots, dullness, and sagging), mainly from the perspective of cosmetic aging, especially from a dermatological perspective.

[0023] However, none of these patent documents apply the concept of anti-glycation to skin or hair growth agents related to the scalp and hair.

[0024] On the other hand, regarding food-related materials, with changes in lifestyles, dietary habits have undergone various changes, and demands have become more diversified.

[0025] In this context, with anti-aging as a central theme, and against the backdrop of increasing health awareness, people place high hopes on functional ingredients that combine nutritional value with multiple physiological activities, and mushrooms are receiving more attention than ever before.

[0026] In view of this, the applicant focused on studying the unique characteristics of white hairy fungus produced by the mutation of hairy fungus (Auricularia polytricha (Mont.) Sacc.) and completed an invention as a multifunctional additive (Patent Document 8).

[0027] There are existing cases of developing hairy fungus into a health-promoting ingredient containing vitamin D (e.g., Patent Document 9). However, unlike the present invention, it does not focus on anti-glycation nutrients and therefore fails to meet consumer expectations for the material.

[0028] As mentioned above, considering its versatility in food and beverage and skin applications, it is of great significance to provide an anti-aging material derived from natural products that has an inhibitory effect on AGE production.

[0029] Existing technical documents Patent documents Patent Document 1: Japanese Patent Application Publication No. 7-278012 Patent Document 2: Japanese Patent Application Publication No. 9-67262 Patent Document 3: Japanese Patent Application Publication No. 2013-116887 Patent Document 4: Japanese Patent Application Publication No. 2005-255527 Patent Document 5: Japanese Patent Application Publication No. 2009-96773 Patent Document 6: Japanese Patent Application Publication No. 2013-139392 Patent Document 7: Japanese Patent Application Publication No. 2014-37399 Patent Document 8: Japanese Patent Application Publication No. 2020-124178 Patent Document 9: Japanese Patent Application Publication No. 2022-121877 Summary of the Invention

[0030] The present invention aims to solve the aforementioned unresolved problems. The present invention addresses the aforementioned unresolved problems and aims to provide a functional material and its uses based on the characteristics of white hairy fungus produced by a mutation of Auricularia polytricha (Mont.) Sacc.

[0031] More specifically, the object of this invention is to emphasize so-called upcycling when selecting materials derived from natural products, in order to provide a material with anti-glycation inhibition (hereinafter referred to as "AGE generation inhibition") of protein glycation in vivo, which is helpful for use in topical formulations (such as cosmetics) that take into account skin affinity and in food and beverage products that take into account nutrition.

[0032] Problem-solving methods To solve the above problems, the inventors continuously explored various materials and finally discovered that white hairy fungus produced by the mutation of Auricularia polytricha (Mont.) Sacc. showed the desired effect, and thus completed the present invention.

[0033] In other words, according to the present invention, the desired purpose is achieved by the following configurations (1) to (6).

[0034] (1) A functional material derived from white hairy fungus that has an inhibitory effect on AGE production, wherein the white hairy fungus is produced by mutation of hairy fungus.

[0035] (2) The functional material derived from white hairy fungus according to (1) comprises vitamin D2 (ergocalciferol) obtained by irradiating white hairy fungus produced by mutation of hairy fungus with ultraviolet B, and free amino acids that remain stable in said white hairy fungus even after irradiation with ultraviolet B.

[0036] (3) The functional material derived from white hairy fungus according to (2), wherein the free amino acid composition includes at least the following amino acids: histidine, serine, arginine, glycine, aspartic acid, glutamic acid, threonine, alanine, γ-aminobutyric acid, proline, cysteine, lysine, tyrosine, methionine, valine, isoleucine, leucine, phenylalanine and tryptophan.

[0037] (4) A food or beverage product comprising the functional material described in (1) and / or (2) as an active ingredient.

[0038] (5) A topical preparation comprising the functional material according to (1) and / or (2) as an active ingredient.

[0039] (6) The functional material derived from white hairy wood ear fungus, the food and beverage product, and the topical preparation according to any one of (1) to (5), wherein the part of the functional material derived from white hairy wood ear fungus used is the wood ear stalk.

[0040] Effects of the invention This invention relates to a functional ingredient derived from a white, hairy fungus produced by a mutation of Auricularia polytricha (Mont.) Sacc. Because this functional ingredient also has AGE-inhibiting effects, it has a wide range of applications and can be used as an additive in a form acceptable to food and beverages for anti-aging purposes, or in a form acceptable for skin applications.

[0041] Furthermore, the material of this invention was developed with nutritional considerations in mind. In particular, after exposure to ultraviolet B, a large amount of vitamin D2 (ergocalciferol) is produced, and even after exposure to ultraviolet B, the free amino acids that are stably present in white wood ear fungus are retained and present. Therefore, it is also helpful as a raw material suitable for the aforementioned anti-aging supplements.

[0042] Furthermore, a significant feature of this invention is its unique properties and the novel functions inherent in the mutant white hairy fungus itself. In particular, due to its viscous components, it can be used as a raw material for food, beverages, and topical preparations such as cosmetics, and facilitates production utilizing its various known functions such as antioxidant, moisturizing, elastic, swelling, and emulsifying properties.

[0043] The aforementioned viscosity properties vary considerably depending on the part used, and the utilization of the stem allows for its application in a variety of novel foods and beverages. While the stem is typically discarded as waste or composted, from a food waste perspective, it represents a significant application as a so-called upgraded remanufacturing material that fully utilizes all parts. Attached Figure Description

[0044] Figure 1 This is a schematic diagram of the process of utilizing the waste portion of the present invention.

[0045] Figure 2 This is a schematic diagram showing the results of an AGE inhibition test.

[0046] Figure 3 This is a schematic diagram showing the basic mechanism by which AGEs inhibit AGEs.

[0047] Figure 4 This is a schematic diagram comparing the changes in vitamin D2 content between white and hairy wood ear fungus.

[0048] Figure 5 The diagrams show a comparison of the water absorption and retention capacity of white hairy fungus and hairy fungus, as well as a comparison of the water retention capacity of white hairy fungus and sodium hyaluronate.

[0049] Figure 6 This is a schematic diagram illustrating the two types of raw materials (dried raw material and raw raw material) used in the production process of white wood ear fungus extract. Detailed Implementation

[0050] This invention is a multifunctional raw material that expands its applications by utilizing white hairy fungus produced by a mutation of hairy fungus, while focusing on anti-aging.

[0051] Its utilization is unrestricted, encompassing a wide range of forms including the raw material itself, pulverized products, and extracts. From an upgrading and remanufacturing perspective, the utilized parts include not only the main body but also all conventionally unused parts of the wood ear stem.

[0052] Regarding the pre-treatment steps before manufacturing, firstly, after harvesting the white wood ear mushrooms, wash them with water, then dry them at a temperature of up to 70°C for 5 hours, and blanch them for 4 minutes. The processing temperature and drying time before blanching can be set arbitrarily according to the intended use.

[0053] When using only the stem of the wood ear mushroom, it should be removed after the above washing process.

[0054] When commercializing the powder, conventional pulverizers can be used, but to prevent browning after processing, it is preferable to perform instantaneous pulverization under a nitrogen atmosphere, or to use a cryogenic dry pulverizer (e.g., the Centri DryMill manufacturing method manufactured by Micro Powtec). The particle size of the powder can vary depending on the application, and can be achieved using the methods disclosed in Patent Document 8, which is an invention of the applicant.

[0055] However, when using only the stems of wood ear mushrooms to make jelly or as a side dish, it is preferable to use a food processor for a short processing time in order to intentionally preserve their specific textures, such as consistency, crispness on the tongue, and chewiness.

[0056] When used as an extract for various purposes, extraction with hot water after pulverization is preferred from the perspective of viscosity adjustment and extraction efficiency, but the specific conditions can be chosen arbitrarily.

[0057] According to the present invention, an anti-glycation effect (AGE production inhibition) was discovered in white hairy fungus produced by hairy fungus mutation, and an anti-aging functional material of great significance was provided.

[0058] As mentioned in the background section, glycation refers to a non-enzymatic chemical reaction between carbohydrates and proteins (amino acids). Because glycation can trigger various diseases in the body, inhibiting this reaction is of great importance.

[0059] Furthermore, according to the present invention, it has been found that white hairy fungus produced by irradiation of hairy fungus with ultraviolet B still contains a large amount of vitamin D2 (ergocalciferol) and free amino acids that are stably present in white hairy fungus, and does not decompose.

[0060] Here, vitamin D2 is a product derived from ergosterol (provitamin D). Amino acids encompass a wide variety of components, including essential amino acids. Specifically, they contain a large number of free amino acids composed of at least the following: histidine, serine, arginine, glycine, aspartic acid, glutamic acid, threonine, alanine, γ-aminobutyric acid, proline, cysteine, lysine, tyrosine, methionine, valine, isoleucine, leucine, phenylalanine, and tryptophan.

[0061] These findings have the following two very important implications: (1) Its application as a nutrient can be expanded.

[0062] (2) It is expected to play a complementary role in inhibiting AGE.

[0063] The above (1) can be widely used, for example, in nutritional supplements for nursing care. Because it contains ingredients that maintain viscosity, it can be cooked and processed into easily swallowable forms, such as gels or liquids, and used for swallowing food.

[0064] Furthermore, since this material also contains valine, leucine, and isoleucine (common ingredients in so-called BCAA supplements), it is expected to be applicable in the field of supplements.

[0065] Regarding (2) above, considering that AGE is caused by the binding of amino acids in the body, since supplementing amino acids can inhibit AGE, it can be expected that it has a so-called dual effect: both supplementing the lacking amino acids and inhibiting the reduction in the number of amino acids. This is highly reasonable and important in terms of metabolic mechanisms.

[0066] In other words, according to claims 1 to 3 of the present invention, the following functional materials are provided.

[0067] (1) A functional material derived from white hairy fungus that has an inhibitory effect on AGE production, which is produced by mutation of hairy fungus.

[0068] (2) The functional material derived from white hairy fungus according to (1) contains vitamin D2 (ergocalciferol) obtained by irradiating white hairy fungus produced by mutation of hairy fungus with ultraviolet B, and free amino acids that remain stable in white hairy fungus even after irradiation with ultraviolet B.

[0069] (3) The functional material derived from white hairy fungus according to (2), wherein the free amino acid composition includes at least the following amino acids: histidine, serine, arginine, glycine, aspartic acid, glutamic acid, threonine, alanine, γ-aminobutyric acid, proline, cysteine, lysine, tyrosine, methionine, valine, isoleucine, leucine, phenylalanine and tryptophan.

[0070] It is natural to provide application products based on the functions discovered in this invention, and it is also permissible to provide application products that include the functions disclosed in Patent Document 8.

[0071] As stated above, the main body, the pulverized product, and the extract can all be optionally used as characteristics of the material; however, regarding the extract, due to its tasteless, odorless, colorless, and transparent nature, and the ability to be appropriately viscous, it can replace egg whites when rice flour is used instead of wheat flour, achieving the same texture and making it an effective anti-allergy food ingredient. Alternatively, as an egg substitute, the material can be used as a material for so-called plant-based foods.

[0072] This pulverized product possesses expansion, retention, and emulsification functions, and imparts elasticity. Therefore, when applied to food, it can provide processed foods with a texture similar to mochi. When added to mochi products or processed foods using rice flour, it provides high-quality products whose firmness or softness does not change over time.

[0073] In other words, as mentioned above, when added to or mixed as a food ingredient, it can not only improve the texture of existing processed foods, but also maintain their quality and extend their shelf life.

[0074] Furthermore, white wood ear mushrooms are known to be rich in dietary fiber. Therefore, the water-retention capacity of dietary fiber can retain moisture, thus maintaining the softness of mochi, bread dough, etc. The extract of this application has excellent permeability and affinity for food ingredients; therefore, for example, it can effectively improve the texture of meats with high sinewiness and toughness.

[0075] Furthermore, what is very interesting is that, in addition to being added as a food ingredient, this invention can also be used as a quality preservative.

[0076] In other words, by packaging the powder of the present invention in a moisture-absorbing packaging material to form a small bag, and then wrapping the small bag together with the processed food, excess moisture can be absorbed, thereby playing a role in preventing mold.

[0077] There are no particular restrictions on its application in the food industry. Specific examples include dietary supplements (such as beverages and nutritional supplements), foods for specific health purposes, foods with functional claims, health foods, and various general processed foods, such as soft drinks, soups, chiffon cakes, Swiss rolls, pizzas, pound cakes, dorayaki, steamed buns, meat buns, fried dumplings, shumai, noodles, donuts, pull-apart bread, macarons, biscuits, puddings, jellies, Bavarian cream, candies, gummies, chewing gum, ice cream, and gelato.

[0078] In these examples, it can be used to increase the consistency of liquid foods such as soft drinks and soups, and to adjust the texture of thixotropic foods such as puddings, jellies, and Bavarian cream. For solid or semi-solid foods such as candies, gummies, chewing gum, ice cream, and gelato, it can be used to adjust the tactile sensation, such as the feel on the tongue.

[0079] For chiffon cakes, Swiss rolls, pizzas, pound cakes, dorayaki, steamed buns, meat buns, fried dumplings, shumai, noodles, donuts, and hand-torn bread, in addition to giving the dough elasticity and adjusting its texture, it can also be used to adjust the texture and feel of the fillings or toppings when these foods contain them. Other uses include direct mixing into fresh fish products (such as fish meat, hamburgers, and fish cakes) to make processed products, and smoked white wood ear mushrooms themselves are also included within the scope of this invention.

[0080] From the perspective of fully demonstrating the material properties of the present invention, processed products made from grains, fish, meat, etc., and processed products made from wheat flour, rice flour, etc. are particularly preferred.

[0081] In terms of its form, the functional raw materials of the present invention can be configured in food and beverage acceptable forms, such as active ingredients or premixes, for formulation into capsules, tablets, granules, powders, syrups, liquids and gels, in market transactions and logistics.

[0082] As for excipients, a variety of acceptable liquid and solid raw materials suitable for oral administration can be used, but sweeteners must be carefully selected considering their anti-glycation effect. That is, sweeteners that are less prone to glycation are preferred, such as sugar alcohols such as sorbitol, maltitol, erythritol, lactitol, xylitol, trehalose, and steviol glycosides, as well as monk fruit extract and the synthetic sweetener aspartame.

[0083] In other words, according to claim 4 of the present invention, a food and beverage product is provided, comprising the functional material of claim 1 and / or the functional material of claim 2 formulated as an active ingredient.

[0084] As oral supplements for food and beverages, etc., known nutritional supplements and blood circulation promoters may be used in appropriate combinations without compromising the effects of the present invention.

[0085] Examples of nutritional supplements include turtle extract, salamander extract, oriental bezoar tincture, deer antler extract, Acanthopanax senticosus extract, Eucommia ulmoides extract, and Polygonum multiflorum extract.

[0086] Examples of blood circulation enhancers include glucosyl hesperidin, citrulline, vitamin E nicotinic acid, nicotinic acid, nicotinamide, benzyl nicotinic acid, ginger tincture, capsicum tincture, and rosmarinic acid.

[0087] When applied to formulations such as cosmetics, its emulsifying, swelling (matrix-forming) and maintenance functions enable formulation designs using ingredients with low skin irritation, providing formulations suitable even for people with delicate skin.

[0088] In addition, it is useful for preventing skin aging because it has an AGE inhibitory effect.

[0089] The functional ingredients of the present invention can be formulated as active ingredients suitable for topical or systemic skin application, such as creams, ointments, emulsions, emollients, pastes, lotions, emulsions, fragrances, gels, powder / liquid / solid bath products, cleansers, shampoos, rinses, conditioners, and toners.

[0090] As described above, because the white hairy fungus of the present invention has excellent emulsifying properties, the basic properties can be guaranteed without the use of surfactants in the emulsified matrix, and a widely applicable formulation suitable for consumers with sensitive skin can be provided. Furthermore, the powder itself is white, which does not impair the desired appearance.

[0091] Furthermore, in transparent formulations, when prepared as an aqueous solution, it can be mixed as a colorless and transparent extract with high moisturizing effect, and its appearance is not compromised in terms of formulation design.

[0092] Furthermore, adjusting the concentration arbitrarily gives the product high viscosity; for example, it can reduce the use of thickeners and other additives.

[0093] The topical formulations of this invention include various forms that fall under the categories of pharmaceuticals, quasi-pharmaceuticals, and cosmetics, and there are no particular limitations on dosage forms as long as they do not impair the purpose of this invention.

[0094] Regarding the mixed matrix (excipients) in formulation design, a variety of liquid and solid raw materials suitable for skin application can be used.

[0095] Regarding the matrix, if it is an emulsion-type matrix, the material of this invention has excellent emulsifying properties, thus eliminating the need for surfactants, making it a very convenient material for low-irritant formulation design. Even if the use of surfactants is unavoidable, their total amount can be reduced.

[0096] Furthermore, as mentioned above, the extract derived from the material according to the present invention has a specific viscosity and exhibits gelling ability due to its synergistic effect when used in conjunction with a thickener, thus allowing for the design of formulations that maintain elasticity. The elastic strength can be arbitrarily adjusted according to the type and amount of thickener added.

[0097] Examples of suitable thickeners include gum arabic, carrageenan, ebony gum, tragacanth gum, locust bean gum, quince seed (quince), casein, dextrin, gelatin, sodium pectate, sodium alginate, methylcellulose, ethylcellulose, carboxymethylcellulose (CMC), hydroxyethylcellulose, hydroxypropylcellulose, polyvinyl alcohol (PVA), polyvinyl alcohol (PVM), polyvinylpyrrolidone (PVP), sodium polyacrylate, carboxyvinyl polymers, carob gum, guar gum, tamarind gum, dialkyldimethylammonium sulfate cellulose, xanthan gum, magnesium aluminum silicate, bentonite, and lithium montmorillonite.

[0098] At this point, various general additives, such as functional ingredients, moisturizers, and antioxidants, can be added as needed without compromising the purpose of the invention.

[0099] In other words, according to claim 5 of the present invention, a topical formulation is provided, characterized in that it contains the functional material of claim 1 and / or the functional material of claim 2 as an active ingredient.

[0100] Examples of moisturizers include polyols such as urea, glycerin, propylene glycol, 1,3-butanediol, sorbitol, mannitol, polyethylene glycol, and dipropylene glycol; ceramides; heparin-like substances; sodium hyaluronate; sodium PCA; collagen; collagen peptides; elastin; chondroitin sulfate; glucosamine; fucoidan; trimethylglycine; betaine; and seaweed extracts (alginate). The combined use of these moisturizers is an effective option for formulation design, particularly for improving rough skin that may occur during dieting.

[0101] The types of collagen mentioned above are not particularly limited, but considering absorbability, collagen peptides are preferred, especially oligopeptides (peptide chain length less than 10) composed of "proline-hydroxyproline" and / or "hydroxyproline-glycine" and determinate collagen.

[0102] Hyaluronic acid is a high molecular weight polysaccharide in which N-acetylglucosamine and glucuronic acid are linked by a β-1,4 glycosidic bond. Preferably, a low molecular weight product (average molecular weight of about 100,000) obtained by enzymes such as hyaluronidase is used.

[0103] Elastin is an elastic fiber that serves to support collagen fibers isolated from mammals such as pigs and cattle, as well as fish such as tuna and salmon. Considering in vivo absorption, elastin with an average molecular weight of approximately 100,000 is preferred, obtained through enzymatic degradation by proteolytic enzymes such as elastase, pepsin, or streptomycin, or by acid hydrolysis. Furthermore, from the perspective of solubility, elastin with an average molecular weight of approximately 20,000 is preferred.

[0104] Chondroitin sulfate is a simple substance, such as chondroitin 6-sulfate and chondroitin 4-sulfate, or mixtures thereof. Its molecular weight is not particularly limited, but considering in vivo absorption, an average molecular weight of 50,000 is preferred. In this invention, salts of chondroitin sulfate may also be used; the type of salt is not particularly limited, but sodium or potassium salts are preferred.

[0105] Examples of preservatives include benzoates, sorbates, dehydroacetates, p-hydroxybenzoates, benzalkonium chloride, juniper alcohol, resorcinol, ethanol, and phenoxyethanol, but it is preferred to use substances of natural origin as their components.

[0106] Examples of antioxidants include butylated hydroxytoluene, butylated hydroxyanisole, propyl gallate, and ascorbic acid, but like preservatives, they also preferably use substances of natural origin as their components.

[0107] In addition to the active ingredients of this invention, other functional ingredients commonly used in topical preparations may be selectively added, provided that these ingredients do not impair the effects and purpose of this invention. Specifically, examples include anti-inflammatory agents such as lysozyme chloride, glycyrrhizic acid or its salts, allantoin; immunostimulants such as β-glucan; peripheral vasodilators such as androgens (methyltestosterone), glucosyl hesperidin, citrulline, vitamin E nicotinic acid ester, nicotinic acid, nicotinamide, benzyl nicotinate, ginger tincture, Japanese swert extract, and capsicum tincture; antibacterial agents such as juniper alcohol, benzalkonium chloride, and undecenoic acid; placental extracts, liver extracts, oyster extracts, marshmallow extracts, cyperus extracts, artemisia extracts, aloe extracts, cyperus extracts, Artemisia annua extracts, and various animal extracts. Extracts from plant and microbial sources, such as aloe vera extract, red ginseng extract, deer antler and Ganoderma lucidum extract, banana peel extract, geranium extract, coltsfoot root extract, lily extract, yarrow extract, dried pumpkin seed extract, sage extract, celery extract, soybean extract, thyme extract, dried citrus peel extract, coriander extract, coltsfoot extract, peony extract, kelp extract, lemon balm extract, grapefruit extract, resveratrol, lactic acid bacteria culture extract, and fermented vegetable, fruit and wild grass extracts (hereinafter referred to as wild grass fermented extracts). The raw materials for this wild plant fermentation extract include, for example, mugwort, houttuynia cordata, aloe vera, turmeric, ginseng, wolfberry, coix seed, St. John's wort, bamboo grass, lychee, dandelion root, lychee, peach leaf, ginkgo leaf, loquat seed, honeysuckle, fig leaf, senna leaf, safflower, eleutherococcus senticosus, gynostemma pentaphyllum, monk fruit, eucommia leaf, angelica dahurica, loquat leaf, moroximate, wolfberry, cinnamon, wild hawthorn, plantain, turmeric, licorice, pine needle, nandina leaf, horsetail, Solomon's seal, dayflower, windleaf tree, four-angled grass, yam, persimmon leaf, etc. In addition, other suitable plants can be selected according to specific uses, such as: quince, perilla leaves, orange peel, ginger, mulberry leaves, andrographis paniculata, kumquat, onion peel, Panax notoginseng, Platycodon grandiflorus, red dates, Saladilla, chili peppers, yew berries, red ginseng, rooibos tea, amla fruit, centella asiatica, cactus, Brazilian privet, Japanese clematis, cat's claw grass, kelp, sea lettuce, oranges, apples, pineapples, grapes, figs, plums, melons, grapefruits, lemons, apricots, carrots, onions, parsley, burdock, garlic, scallions, cabbage, shiitake mushrooms, etc.

[0108] In addition, other examples of AGE production inhibitors include known plant extracts such as flax, Solomon's seal, olive, asarum, linden, meadowsweet, Japanese swert, elderberry, rose, sunflower, purple swert, saxifrage, and burnet root.

[0109] In this invention, the normally unused stem portion of the wood ear mushroom is actively utilized to make full use of the material. This is an upcycling method aimed at reducing so-called "food waste." Upcycling typically refers to increasing the value of a product by giving new value to waste or unused, unwanted items, but this invention creates value in the unused stem portion of the wood ear mushroom.

[0110] That is, according to claim 6, a functional material derived from white hairy fungus, a food and beverage product, and a topical preparation are provided, wherein the functional material derived from white hairy fungus is used in the fungus stem.

[0111] Using the stem portion of the wood ear mushroom as a functional material has many advantages. For example... Figure 1 As shown, the utilization process is as follows: After washing and pulverizing, extraction is performed using hot water. The extract and residue are then separated and can be used according to the desired purpose. The extract is rich in various beneficial components and can be directly used in beauty essences or bath additives. To improve storage stability, it can be freeze-dried into special sheet materials, or it can be prepared for use immediately. Due to the unique texture of the extraction residue, it can also be used as a dessert jelly.

[0112] Both the extract and residue can be pre-irradiated with ultraviolet light (UVB) to make them functional materials rich in vitamin D2 and amino acids, making them convenient to provide as nutritional supplements.

[0113] In other words, according to the present invention, the desired objective is achieved by the following configurations (1) to (6).

[0114] (1) A functional material derived from white hairy fungus that has an inhibitory effect on AGE production, wherein the white hairy fungus is produced by mutation of hairy fungus.

[0115] (2) The functional material derived from white hairy fungus according to (1) contains vitamin D2 (ergocalciferol) obtained from white hairy fungus produced by mutation of hairy fungus by ultraviolet B irradiation and free amino acids that remain stable in white hairy fungus even after ultraviolet B irradiation.

[0116] (3) The functional material derived from white hairy fungus according to (2), wherein the free amino acid components include at least the following amino acids: histidine, serine, arginine, glycine, aspartic acid, glutamic acid, threonine, alanine, γ-aminobutyric acid, proline, cysteine, lysine, tyrosine, methionine, valine, isoleucine, leucine, phenylalanine and tryptophan.

[0117] (4) A food or beverage product comprising the functional material described in (1) and / or (2) as an active ingredient.

[0118] (5) A topical preparation comprising the functional material according to (1) and / or (2) as an active ingredient.

[0119] (6) The functional material, food and beverage products and topical preparations derived from white hairy wood ear fungus according to any one of claims (1) to (5), wherein the application site of the functional material derived from white hairy wood ear fungus is the wood ear stalk.

[0120] Example The functions of the present invention will be specifically illustrated by the following embodiments, but the scope of the present invention is not limited to these embodiments.

[0121] <Test Example 1> AGE Production Inhibition Test (Testing Method) (1) Reagents, consumables and measuring instruments • Collagen Anti-Glycation Assay Kit (Glucose / Fructose) (Cosmo Bio Co., Ltd.) • 96-well plate, F-Bottom MicroCLEAR, black, TC, Greiner (Green Bio Research) • SH-9000Lab microplate reader for downward fluorescence measurement (Corona Electric Co., Ltd.) (2) Test samples and experimental procedures Heat-treated powder was used as the sample. The powder was prepared into sample solutions at concentrations of 0.005, 0.05, 0.5, and 5 mg / mL for evaluation. The experimental procedure is as follows.

[0122] Add 50 μL of neutralized collagen solution to a 96-well plate and incubate overnight at 37°C. Then, add 10 μL of aminoguanidine solution and sample solution, followed by 50 μL of buffer and fructose solution.

[0123] After stirring, the fluorescence intensity measured at an excitation wavelength of 370 nm and a fluorescence wavelength of 440 nm was defined as I0, and measurements were taken three times per week. The rate of glycated collagen formation was calculated based on the obtained absorbance. The formula is as follows: Glycated collagen production rate (%) = Fss: Absorbance of fructose group sample Fbs: Absorbance of the buffer group sample Fs: Absorbance of the fructose control group Fb: Absorbance of the buffer group control (Results and Discussion) Figure 2 shows the results of glycated collagen production rate after 3 weeks.

[0124] This represents the proportion of sample concentrations with the blank group as 100%. The glycated collagen production rate of *Auricularia auricula-judae* decreased with increasing concentration, and was superior to that of *Auricularia auricula-judae* at any concentration. At a concentration of 5 mg / mL, the glycated collagen production rate was 10%, the same as the positive control aminoguanidine (AG).

[0125] On the other hand, the glycated collagen production rate of white Auricularia auricula-judae at a concentration of 5 mg / mL was 59%. These results indicate that white Auricularia auricula-judae contains more substances that inhibit collagen glycation.

[0126] Figure 3 The mechanism of anti-glycation action was demonstrated. It inhibits glycation by suppressing the reaction of the intermediate 3-deoxyglucose ketone with amino-containing compounds to form AGEs, thereby preventing AGE formation and exerting an inhibitory effect.

[0127] As described below, white auricularia auricula-judae is found to be rich in essential amino acids. Based on the aforementioned anti-glycation mechanism, it is speculated that the inhibitory effect of white auricularia auricula-judae is related to the amino groups contained in its components. By optimizing the application of the materials of this invention (topical and / or oral forms), the desired effects of this invention can be expected to be achieved.

[0128] <Test Example 2> Determination of Vitamin D2 Content (Testing Method) The transformation of white hairy fungus after heat treatment according to the present invention was confirmed by irradiating it with varying (including substantial enhancement) ultraviolet B (285 nm) energy.

[0129] (Results and Discussion) The results are shown in Table 1. The irradiation energy was set from 1 to 5 for enhanced UVB (285 nm). Before irradiation, vitamin D2 (ergocalciferol) was absent; only the vitamin D2 precursor ergosterol was detected. However, by increasing the irradiation intensity, the ergosterol content decreased, and vitamin D2 was detected in an intensity-dependent manner.

[0130] [Table 1] Under the same conditions, the amount of vitamin D2 (ergocalciferol) in the samples was determined using heat-treated Auricularia auricula-judae. The results showed that Auricularia auricula-judae also exhibited the same trend, but the absolute content comparison showed that it was lower than that of white Auricularia auricula-judae, thus demonstrating the superiority of the material of the present invention (Table 2 and...). Figure 4 ).

[0131] [Table 2] <Test Example 3> Determination of Free Amino Acids (Testing Method) As shown below, the types and contents of free amino acids in each sample were determined and compared using white hairy fungus after heat treatment according to the present invention and heat-treated hairy fungus as a comparison.

[0132] (Results and Discussion) The results are shown in Table 3. The content of free amino acids remained stable after ultraviolet irradiation. Furthermore, the total amount of free amino acids in white auricularia auricularia was higher than that in black auricularia auricularia.

[0133] [Table 3] <Test Example 4> Water Absorption and Retention Test (1) Comparison with hairy wood ear fungus: 10 g of dried white hairy wood ear fungus (Bai Meirong; registered trademark) was placed in boiling water, and after turning off the heat, it was left to stand and the changes in its water absorption and water retention capacity were observed.

[0134] The results showed that after 17 hours, the white auricularia auricula-judae swelled to approximately 600 g after absorbing water. Measurements of water retention after standing confirmed that even after 6 days (144 hours), the white auricularia auricula-judae still retained 45% water, significantly better than the white auricularia auricula-judae. The results are shown in Table 4. Figure 5 (superior).

[0135] [Table 4] (2) Comparison with sodium hyaluronate Disperse each freeze-dried sample in water to 0.2%, take 1 mL of the dispersion and immerse it on glass fiber filter paper (ADVANTECGS-25, φ47 mm) (n=4), let it stand for 8 hours at 25℃ / 55% relative humidity, and calculate the water retention capacity according to the following formula based on the residual moisture content.

[0136] [Water retention = weight of residual moisture after 8 hours / weight of sample] Using the methods described above, and comparing with sodium hyaluronate, it was confirmed that the water retention capacity of white hairy wood ear fungus is comparable to that of sodium hyaluronate. Similar excellent water retention properties were also obtained for the less commonly used stem portion of the fungus. These evaluation results are as follows: Figure 5 As shown below.

[0137] The water retention properties of each part are as follows.

[0138] 1. Powder: 112.6 2. Hot water extract: 201.1 3. Pressurized hot water extract: 222.3 4. Hot water extract of the inedible portion: 216.5 5. Sodium hyaluronate: 221.0 like Figure 6 As shown, when adding raw materials for the extract of the present invention, there are cases where dried raw materials are used and cases where raw raw materials are used, but the conditions are the same even when all raw materials are used without discarding the wood ear stems.

[0139] Furthermore, regarding the manufacturing process (application examples), when using wood ear stems that are not typically used, such as... Figure 1 As shown, there are many applications; for example, using the extracted residue as a dessert jelly is an unprecedented new application.

[0140] <Formulation Example 1> Toner Raw material name (by weight %) 1. White hairy fungus powder: 2.00 2. Ascorbate glucoside: 2.00 3. Kojic acid: 1.00 4. Cucumber extract: 2.00 5. Thyme extract: 0.50 6.5% elastin hydrolysate: 1.00 7. Purified water: appropriate amount <Formulation Example 2> Essence Raw material name (by weight %) 1. Allantoin: 0.50 2. Placental extract: 0.50 3. White hairy fungus powder: 5.00 4. Purified water: appropriate amount <Formulation Example 3> Cream Raw material name (by weight %) 1. Retinol: 1.00 2. Sodium hyaluronate aqueous solution: 2.00 3. Polyethylene glycol 400: 3.00 4. White hairy fungus powder: 5.00 5. Stearic acid: 5.00 6. Avocado oil: 1.00 7. Almond oil: 10.00 8. dl-pyrrolidone sodium carboxylate solution: 5.00 9. Purified water: as needed <Formulation Example 4> Cream Mask Raw material name (by weight %) 1. Retinol: 2.00 2. Arbutin: 1.00 3. Sodium polyacrylate: 5.00 4. White fungus extract (5% aqueous solution): 5.00 5. Myristic acid: 6.00 6. Grapeseed oil: 1.00 7 Jojoba oil: 3.00 8. Olive oil: 2.00 9. Purified water: as needed <Formulation Example 5> Emulsion Raw material name (by weight %) 1. Retinoic acid: 4.00 2. White hairy fungus powder: 3.00 3. Almond oil: 4.00 4. Olive oil: 15.00 5. Xanthan gum: 0.50 6. Purified water: appropriate amount <Formulation Example 6> Hair Care Essence Raw material name (by weight %) 1. Minoxidil: 3.00 2. Ethanol: 7.00 3,1,3-Butanediol: 5.00 4. White fungus extract (2% aqueous solution): 20.00 5. Purified water: appropriate amount <Formulation Example 7> Bath Soap Raw material name (by weight %) 1. Potassium myristate: 4.50 2. White fungus extract (4% aqueous solution): 25.00 3. Egg yolk lecithin: 3.00 5. Glycol distearate: 2.00 6. Dipropylene glycol: 2.00 7. Glycerin: 1.00 8. Purified water: appropriate amount <Formulation Example 8> Soap (Elastic Type) Raw material name (by weight %) 1. Glycerin: 25.00 2. Potassium cocoyl glycinate: 8.00 3. Tamarind gum: 2.00 4. Pentylene glycol: 5.00 5. White fungus extract (0.5% aqueous solution): 5.00 6. Fucoidan extract from sea anemones: 1.50 7. Sodium hyaluronate: 2.50 8. Hydrolyzed collagen: 3.00 9. Purified water: as needed <Formulation Example 9> Jelly Beverage Raw material name (by weight %) 1. Maltodextrin: 4.50 2. High fructose corn syrup: 1.00 3. Citric acid: 0.10 5. Gelling agent (thickening polysaccharide): 2.00 6. White fungus extract (5% aqueous solution): 10.00 (Contains 20% wood ear stem extract residue) 7. Nicotinic acid: 0.01 8. Purified water: appropriate amount <Formulation Example 10> Turkish Delight Raw material name (by weight %) 1. Glutinous rice starch: 22.00 2. White hairy fungus powder: 1.00 3. Sugar: 34.00 4. Maltose: 10.00 5. Plantain seed: 0.20 6. Purified water: appropriate amount <Formulation Example 11> Beverage Raw material name (by weight %) 1. Ascorbic acid maltodextrin: 0.50 2. White fungus extract (4% aqueous solution): 6.00 3. High-fructose corn syrup: 2.00 4. Honey: 0.50 5. Ascorbic acid: 0.30 6. Vitamin B6: 0.005 7. Vitamin B12: 0.001 8. Isoleucine: 0.005 9. Phenylalanine: 0.005 10. Guarana extract: 0.05 11. Fragrance: 0.10 12. Purified water: appropriate amount <Formulation Example 12> Pizza Dough Raw material name (by weight %) 1. Rice noodles: 40.00 2. White hairy fungus powder: 2.00 3. Cane sugar: 5.00 4. Potato starch: 6.50g 5. Dry yeast: 1.00 6. Purified water: 40.00 7. Olive oil: 5.00 8. Salt: 0.50g <Formulation Example 13> Protein-containing food products Raw material name (by weight %) 1. Pea protein: 40.00 2. White hairy fungus powder: 25.00 3. Brown sugar: 4.00 4. Valine: 0.50 5. Leucine: 1.00 6. Arginine: 2.00 7. Tryptophan: 0.50 8. Whey: 3.00 9. Maltodextrin: 24.00

Claims

1. A type of fungus derived from hairy fungus (Auricularia auricula-judae) Auricularia polytricha (Mont.) Sacc. The functional material of white hairy fungus produced by the mutation of ) has an inhibitory effect on the production of advanced glycation end products (AGE).

2. The functional material derived from white hairy fungus according to claim 1, comprising vitamin D2 (ergocalciferol) obtained by irradiating white hairy fungus produced by mutation of hairy fungus with ultraviolet B, and free amino acids that remain stable in said white hairy fungus even after irradiation with ultraviolet B.

3. The functional material derived from white hairy fungus according to claim 2, wherein the free amino acid composition includes at least the following amino acids: histidine, serine, arginine, glycine, aspartic acid, glutamic acid, threonine, alanine, γ-aminobutyric acid, proline, cysteine, lysine, tyrosine, methionine, valine, isoleucine, leucine, phenylalanine, and tryptophan.

4. A food or beverage product comprising the functional material as an active ingredient according to claim 1 and / or claim 2.

5. A topical formulation comprising the functional material according to claim 1 and / or claim 2 as an active ingredient.

6. Functional materials, food and beverage products, and topical preparations derived from *Auricularia auricula-judae* according to any one of claims 1 to 5, wherein, The functional material derived from white hairy wood ear fungus is used in the stem of the fungus.

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