Tabletop cell mechanoporation system

Abstract

The present article is a tabletop device for introducing a cargo(for example, a substance such as DNA, RNA, CRISPR RNPs, polymer, small molecule, carbon nanotube, protein, peptide, nanoparticle, antibody, virus, and labeling molecule)into a cell (for example, a primary blood cell, a primary immune cell, various stem cells, and a cell of an established cell line)using mechanoporation (mechanical disruption). Upon use, firstly, a cartridge holding a cell solution in which cells and the cargo are admixed is placed on the upper part of the present article. Next, the present article is pressurized and a button on the present article is pressed. Thereby, the cell solution passes through a filter within the cartridge and is pushed out into the tube placed beneath the cartridge.When the cell solution passes through the micropores of the filter, the cell membrane is mechanically disrupted, the substance permeability of the cell membrane temporarily increases, and the cargo in the cell solution is introduced into the cells. A cargo can be introduced into cells with low invasiveness and high efficiency without requiring any particular reagent or electric stimulation, and the introduced cells show high survival rates.

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