Muscle satellite cell proliferation promoter and muscle satellite cell proliferation inhibitor

Abstract

To provide a muscle satellite cell proliferation promoter and a muscle satellite cell proliferation inhibitor.SOLUTION: The present invention provides a muscle satellite cell proliferation promoter that contains at least one selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and non-fermented tea leaf extract and provides a muscle satellite cell proliferation inhibitor that contains fermented tea leaf extract.SELECTED DRAWING: None

Description

[Technical field] 【0001】 The present invention relates to an agent for promoting muscle satellite cell proliferation, a product for promoting muscle satellite cell proliferation, an agent for inhibiting muscle satellite cell proliferation, and a product for inhibiting muscle satellite cell proliferation. [Background technology] 【0002】 In the bodies of mammals, including humans, myoblasts differentiate into myotubes, which fuse to become muscle fibers, and connective tissue fills the spaces between the cells to form muscle bundles, which then gather together to form muscles. Muscles play an important role in energy metabolism, the intake of sugar and other substances into the body, daily movements such as walking and maintaining posture, and physical activities such as exercise, but they are known to decrease with age. 【0003】 Muscle satellite cells exist between the cell membrane and the basement membrane of muscle fibers. When activated, muscle satellite cells differentiate into myoblasts and then into muscle cells. Multiple muscle cells fuse together to form myotubes, which then form muscle fibers. 【0004】 Although a satellite cell differentiation promoter containing hawthorn or rosea has been proposed to improve muscle mass and strength (Patent Document 1), the proliferation of satellite cells has not been fully studied. To prevent the decline in quality of life associated with aging and live longer and healthier lives, external and oral products that promote and inhibit the proliferation of satellite cells are desired. [Prior art documents] [Patent documents] 【0005】 [Patent Document 1] JP 2014-15429 A Summary of the Invention [Problem to be solved by the invention] 【0006】 The present invention aims to provide a satellite cell proliferation promoter containing one or more selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract, as well as a satellite cell proliferation inhibitor containing a fermented tea leaf extract. [Means for solving the problem] 【0007】 The present inventors have unexpectedly found that a composition containing one or more selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract promotes the proliferation of muscle satellite cells and has a satellite cell proliferation promoting effect. On the other hand, they have found that a composition containing a fermented tea leaf extract inhibits the proliferation of muscle satellite cells and has a satellite cell proliferation inhibiting effect. The present invention is based on this finding and provides the following inventions. 【0008】 [1] A muscle satellite cell proliferation promoter comprising one or more active ingredients selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract. [2] The muscle satellite cell proliferation promoter described in [1], wherein the soybean is a soybean seed. [3] The muscle satellite cell proliferation promoter described in [1], wherein the semi-fermented tea leaves are oolong tea leaves and the unfermented tea leaves are Uji tea leaves and / or matcha leaves. [4] An external composition for promoting the proliferation of muscle satellite cells, comprising as an active ingredient one or more selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract. [5] The external composition for promoting muscle satellite cell proliferation described in [4], wherein the soybean is a soybean seed. [6] The external composition for promoting muscle satellite cell proliferation described in [4], wherein the semi-fermented tea leaves are oolong tea leaves and the unfermented tea leaves are Uji tea leaves and / or matcha leaves. [7] A food or beverage composition for promoting the proliferation of muscle satellite cells, comprising as an active ingredient one or more selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract. [8] The food and beverage composition for promoting muscle satellite cell proliferation described in [7], wherein the soybean is a soybean seed. [9] The food and beverage composition for promoting the proliferation of muscle satellite cells described in [7], wherein the semi-fermented tea leaves are oolong tea leaves and the unfermented tea leaves are Uji tea leaves and / or matcha leaves.

[10] An inhibitor of muscle satellite cell proliferation containing fermented tea leaf extract as an active ingredient.

[11] The muscle satellite cell proliferation inhibitor described in

[10] , wherein the fermented tea leaves are black tea leaves.

[12] The muscle satellite cell proliferation inhibitor described in

[11] , wherein the black tea leaves are Hongfugui tea leaves.

[13] An external composition for inhibiting the proliferation of muscle satellite cells, comprising a fermented tea leaf extract as an active ingredient.

[14] The external composition for inhibiting muscle satellite cell proliferation described in

[13] , wherein the fermented tea leaves are black tea leaves.

[15] The external composition for inhibiting muscle satellite cell proliferation described in

[14] , wherein the black tea leaves are Benifuki tea leaves.

[16] A food or beverage composition for inhibiting the proliferation of muscle satellite cells, comprising a fermented tea leaf extract as an active ingredient.

[17] The food and beverage composition for inhibiting muscle satellite cell proliferation described in

[16] , wherein the fermented tea leaves are black tea leaves.

[18] The food and beverage composition for inhibiting muscle satellite cell proliferation described in

[17] , wherein the black tea leaves are Benifuki tea leaves. Effect of the Invention 【0009】 The satellite cell proliferation promoter of the present invention contains one or more selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract, and therefore promotes the proliferation of satellite cells, while the satellite cell proliferation inhibitor contains a fermented tea leaf extract, and therefore can inhibit the proliferation of satellite cells. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS 【0010】 Hereinafter, an embodiment of the present invention will be described in detail. However, the present invention is not limited to the following embodiment. 【0011】 [1. Muscle satellite cell proliferation promoter] The muscle satellite cell proliferation promoter of this embodiment contains one or more selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract. 【0012】 Soybean means a plant of the legume family. The soybean extract of the present invention may be any part, and one or more parts may be selected and used. In particular, an extract of seeds is preferable. The soybean extraction method and extraction solvent are not particularly limited, and known techniques can be used. The extract may be used as it is, or may be dried into powder or granules, or may be decomposed by treating the extract using known techniques such as enzymatic decomposition. Commercially available products may be used. 【0013】 Tea leaves refer to the leaves of plants belonging to the family Theaceae. Tea leaves are classified into semi-fermented tea leaves, non-fermented tea leaves, and fermented tea leaves depending on the degree of enzyme reaction in the tea leaves. Specifically, semi-fermented tea leaves such as oolong tea leaves; non-fermented tea leaves such as Uji tea leaves, matcha leaves, and green tea leaves are exemplified. In particular, oolong tea leaves, Uji tea leaves, and matcha leaves are preferred. The method of extracting tea leaves and the extraction solvent of the present invention are not particularly limited, and known techniques can be used. The extract may be used as it is, or may be dried into powder or granules, or may be a decomposition product obtained by treating the extract using known techniques such as enzymatic decomposition. Commercially available products may be used. 【0014】 When a soybean and / or tea leaf extract is obtained using a solvent, any solvent that is commonly used may be used, for example, an extract obtained by extraction with one or more polar solvents such as ethanol and water, a dried extract obtained by drying the extract into powder, or a pulverized product, extract, dried product, etc. of the above obtained by purifying the product through a column or partitioning between solutions to enhance the active ingredients. Commercially available products may also be used. 【0015】 The satellite cell proliferation promoter of the present invention has a satellite cell proliferation promoting effect, and is expected to have an effect of increasing muscle mass. In particular, it is expected to be effective in preventing and / or treating sarcopenia, locomotive syndrome, and physical frailty associated with aging. In addition, because it has the above-mentioned effect, it is expected to increase the muscle under the skin, and prevent and / or improve wrinkles, sagging, and loss of firmness and elasticity of the skin. In particular, the face is expected to be effective in preventing and / or improving eyelid depressions, sagging and puffy tear bags and eye bags, wrinkles and sagging at the corners of the eyes, etc., because the skin around the eyes is particularly thin, and it is expected to be highly effective in preventing and / or improving changes in appearance. 【0016】 The content of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract in the satellite cell proliferation promoter according to the present embodiment is not particularly limited as long as it is within a range that has a satellite cell proliferation promoting effect, and is appropriately set according to the type and content of other blended ingredients, the formulation form of the satellite cell proliferation promoter, etc. From the viewpoint of more significantly exhibiting the effects of the present invention, for example, it is preferably 0.00001 to 5 w / v%, more preferably 0.00002 to 3 w / v%, even more preferably 0.00005 to 1 w / v%, and particularly preferably 0.0001 to 1 w / v%. The dosage to humans is preferably 0.001 to 2000 mg per day, more preferably 0.01 to 1000 mg, even more preferably 0.1 to 500 mg, and particularly preferably 1 to 300 mg. 【0017】 The muscle satellite cell proliferation promoter according to this embodiment may further contain excipients, thickeners, binders, disintegrants, dispersants, surfactants, suspending agents, emulsifiers, stabilizers, gelling agents, sweeteners, sour agents, umami agents, salty agents, bitter agents, colorants, UV protection agents, antioxidants, preservatives, antibacterial agents, pH adjusters, buffers, chelating agents, vitamins, flavorings, additives such as minerals, amino acids or salts thereof, sugars, moisturizers, organic acids or salts thereof, dietary fiber, plant extracts excluding indigo and Phellodendron bark, animal tissue extracts, etc. These can be appropriately selected from known substances and can be produced using conventional methods. 【0018】 The dosage form of the muscle satellite cell proliferation promoter according to this embodiment can be used without being limited to a specific product or property, and can be appropriately prepared by a known method in any dosage form, such as liquid, gel, cream, paste, solid, aerosol, or patch. Examples of liquid forms include lotion, milky lotion, beauty essence, oral liquid, and syrup. Examples of solid forms include sticks, tablets, chewable agents, granules, powders, capsules, and ointments. In addition, although the dosage form may be either an external or internal agent, from the viewpoint of achieving the effects of the present invention, an external agent is preferable. The external agent may be a pharmaceutical or a cosmetic, and is not limited thereto. These can be manufactured using conventional methods. 【0019】 [2. Topical products for promoting satellite cell proliferation] The topical product according to this embodiment has an effect of promoting the proliferation of muscle satellite cells. Thus, as one embodiment of the present invention, there is provided a topical product for promoting the proliferation of muscle satellite cells, which contains one or more selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract. 【0020】 In this embodiment, the content of one or more selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract, the types and contents of other ingredients, the formulation form and uses of the external product, etc. are as explained in [1. Muscle satellite cell proliferation promoter]. 【0021】 [3. Food and beverage products for promoting the proliferation of muscle satellite cells] The food and beverage product according to this embodiment has an effect of promoting the proliferation of muscle satellite cells. Thus, one embodiment of the present invention provides a food and beverage product for promoting the proliferation of muscle satellite cells, which contains one or more selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract. 【0022】 In this embodiment, the content of one or more selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract, the types and contents of other ingredients, the formulation form and uses of the external product, etc. are as explained in [1. Muscle satellite cell proliferation promoter]. 【0023】 [4. Muscle satellite cell proliferation inhibitors] The muscle satellite cell proliferation inhibitor of this embodiment contains a fermented tea leaf extract. 【0024】 Tea leaves refer to the leaves of plants belonging to the family Theaceae. Tea leaves are classified into semi-fermented tea leaves, non-fermented tea leaves, and fermented tea leaves depending on the degree of enzyme reaction in the tea leaves. Specific examples include fermented tea leaves such as black tea leaves. In particular, Hongfugui tea leaves are preferred. The method of extracting the tea leaves and the extraction solvent of the present invention are not particularly limited, and known techniques can be used. The extract may be used as it is, or may be dried into powder or granules, or may be a decomposition product obtained by treating the extract using known techniques such as enzymatic decomposition. Commercially available products may be used. 【0025】 When extracts of tea leaves are obtained using a solvent, any solvent that is commonly used may be used, for example, an extract obtained by extraction with one or more polar solvents such as ethanol and water, a dried extract and powder, or a ground product, extract, dried product, etc., purified by a column or partition between solutions to enhance the active ingredients, etc. Commercially available products may also be used. 【0026】 The satellite cell proliferation inhibitor of the present invention has an inhibitory effect on the proliferation of satellite cells, and is expected to have effects such as inhibiting an increase in muscle mass and inhibiting muscle hypertrophy. In particular, it is expected to be effective in preventing and / or treating changes in appearance caused by exercise or muscle growth. In addition, because it has the above-mentioned action, it is expected to have effects such as preventing and / or treating an increase in face size caused by masseter muscle growth, and preventing and / or treating an increase in arm and leg thickness caused by exercise or training. In particular, since the face is prone to change in impression due to changes in appearance, it is expected to have a great effect on preventing and / or improving changes in appearance. In addition, it is expected to have an effect of regulating muscle mass for the purpose of slimming. 【0027】 The content of the fermented tea leaf extract in the satellite cell proliferation inhibitor according to the present embodiment is not particularly limited as long as it has a satellite cell proliferation inhibitory effect, and is appropriately set according to the type and content of other blended ingredients, the formulation form of the satellite cell proliferation inhibitor, etc. From the viewpoint of more prominently exhibiting the effects of the present invention, for example, it is preferably 0.00001 to 5 w / v%, more preferably 0.00002 to 3 w / v%, even more preferably 0.00005 to 1 w / v%, and particularly preferably 0.0001 to 1 w / v%. The dosage to humans is preferably 0.001 to 2000 mg per day, more preferably 0.01 to 1000 mg, even more preferably 0.1 to 500 mg, and particularly preferably 1 to 300 mg. 【0028】 The muscle satellite cell proliferation inhibitor according to the present embodiment may further contain excipients, thickeners, binders, disintegrants, dispersants, surfactants, suspending agents, emulsifiers, stabilizers, gelling agents, sweeteners, sour agents, umami agents, salty agents, bitter agents, colorants, UV protection agents, antioxidants, preservatives, antibacterial agents, pH adjusters, buffers, chelating agents, vitamins, flavorings, additives such as minerals, amino acids or salts thereof, sugars, moisturizers, organic acids or salts thereof, dietary fiber, plant extracts excluding indigo and Phellodendron bark, animal tissue extracts, etc. These can be appropriately selected from known substances and can be produced using conventional methods. 【0029】 The dosage form of the muscle satellite cell proliferation inhibitor according to the present embodiment can be used without being limited to a specific product or property, and can be appropriately prepared by a known method in any dosage form, such as liquid, gel, cream, paste, solid, aerosol, or patch. Examples of liquid forms include lotion, milky lotion, beauty essence, oral liquid, and syrup. Examples of solid forms include sticks, tablets, chewable agents, granules, powders, capsules, and ointments. In addition, although the dosage form may be either an external or internal agent, from the viewpoint of achieving the effects of the present invention, an external agent is preferable. The external agent may be a pharmaceutical or a cosmetic, and is not limited thereto. These can be manufactured using conventional methods. 【0030】 [5. Topical products for inhibiting satellite cell proliferation] The topical product according to this embodiment has an inhibitory effect on the proliferation of muscle satellite cells. Therefore, as one embodiment of the present invention, there is provided an topical product for inhibiting the proliferation of muscle satellite cells, which contains a fermented tea leaf extract. 【0031】 In this embodiment, the content of fermented tea leaf extract, the types and contents of other ingredients, the formulation form and use of the external product, etc. are as explained in [4. Muscle satellite cell proliferation inhibitor]. 【0032】 [6. Food and beverage products for inhibiting muscle satellite cell proliferation] The food and beverage product according to this embodiment has an inhibitory effect on the proliferation of muscle satellite cells. Therefore, one embodiment of the present invention provides a food and beverage product for inhibiting the proliferation of muscle satellite cells, which contains a fermented tea leaf extract. 【0033】 In this embodiment, the content of fermented tea leaf extract, the types and contents of other ingredients, the formulation form and use of the external product, etc. are as explained in [4. Muscle satellite cell proliferation inhibitor]. EXAMPLES 【0034】 The present invention will be specifically described below based on test examples, but the present invention is not limited thereto. In addition, the blending amounts in the following test examples are shown in mass % unless otherwise specified. In addition, the test conditions such as culture conditions in each test example were the same except for the difference in the plant extract added, unless otherwise specified. In addition, a control without the addition of the plant extract was provided for each test, and the effect of the plant extract of the present invention was accurately evaluated. 【0035】 [Test Example 1: Muscle Satellite Cell Proliferation Test 1] Muscle satellite cell samples were prepared as follows. 1. The cryotube in which muscle satellite cells (HSkMC, manufactured by LIFELINE) had been frozen and stored was removed from the liquid nitrogen tank for cell storage and immersed in a thermostatic bath to thaw the suspension. 2. The suspension was transferred to a 50 mL centrifuge tube, and the inside of the ceramic tube was washed with culture medium. 3. Culture medium (StemLife Sk Comp kit, manufactured by LIFELINE) was added to the suspension to make the total volume 30 mL. 4. Of the total volume of 30 mL, 15 mL was seeded into two T75 flasks. 5. The T75 flask was placed in a CO2 incubator (37.0°C, 5.0% carbon dioxide). 6. After 1 day, the medium was changed, and then every other day. 7. Remove the T75 flask from the CO2 incubator and observe under a phase contrast microscope to confirm that the satellite cells were in a subconfluent state. 8. Satellite cells were detached from the T75 flask by trypsinization and collected in a 50 mL centrifuge tube. The number of viable satellite cells was counted by trypan blue staining. 【0036】 A muscle satellite cell proliferation test was performed as follows. 1. Muscle satellite cells were seeded into a 96-well plate at 2000 cells / 100uL / well and cultured for 24 hours (37.0℃, 5.0% carbon dioxide). 2. The medium was removed by suction, and each plant extract was added at 200 uL / well. The cells were then cultured for 3 days (37.0°C, 5.0% carbon dioxide) in a medium not containing growth factors. 3. After incubation, the medium was removed and the cells were fixed with 100 uL of methanol at 25° C. for 20 minutes. 4. The plate was inverted on a paper towel to remove the methanol, and then washed twice with PBS. 5. After removing the PBS, a blocking solution was added and shaken at room temperature (450 rpm). 6. The blocking solution was removed, and 30 ul of primary antibody (Rb pAb to PAX7 (abcam ab187339) was added and incubated overnight at 4°C. 7. The reaction liquid was discarded and the plate was washed once with PBS. 8.50 ul of secondary antibody (Alexa Fluor 633 goat anti-rabbit IgG (H+L) (Invitrogen A21070)) 1 / 1000 and hoechst33342 1 / 1000 in blocking solution were added, and the plate was placed on a shaker (450 rpm) at room temperature under dark conditions for 1 hour. 9. The liquid was discarded, and the cells were washed once with PBS, after which the cell count was measured using ImageXpress Micro (Molecular Devices). 10. The ratio of the cell number in each test solution to the cell number in the control without added plant extract was calculated as the muscle satellite cell proliferation rate. 【0037】 The results are shown in Table 1. The test solutions containing soybean seed extract and oolong tea leaf extract promoted the proliferation of muscle satellite cells. The test solutions containing soybean seed extract and oolong tea leaf extract further promoted the proliferation of muscle satellite cells. 【0038】 [Table 1] 【0039】 The results are shown in Table 2. The test solutions containing oolong tea leaf extract, Uji tea leaf extract, and Kyoto matcha leaf extract promoted the proliferation of muscle satellite cells. 【0040】 [Table 2] 【0041】 The results are shown in Table 3. The test solution containing Benifuki tea leaf extract inhibited the proliferation of muscle satellite cells. 【0042】 [Table 3] 【0043】 In order to maintain and improve health and appearance, it is important to regulate the quantity and quality of muscles throughout the body, and it is also important to regulate the quantity and quality of muscles necessary for the proper functioning of each part of the body. According to the present invention, the proliferation of satellite cells is promoted by one or more selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract, while the proliferation of satellite cells is inhibited by fermented tea leaf extract, so that the proliferation of satellite cells can be controlled according to the purpose.

Claims

[Claim 1] A muscle satellite cell proliferation promoter containing one or more active ingredients selected from the group consisting of soybean extract, semi-fermented tea leaf extract, and unfermented tea leaf extract. [Claim 2] The muscle satellite cell proliferation promoter according to claim 1, wherein soybeans are soybean seeds, semi-fermented tea leaves are oolong tea leaves, and unfermented tea leaves are Uji tea leaves and / or matcha leaves. [Claim 3] A muscle satellite cell proliferation inhibitor containing fermented tea leaf extract as an active ingredient. [Claim 4] The muscle satellite cell proliferation inhibitor according to claim 3, wherein the fermented tea leaves are Benifuki tea leaves.