Antipathogenic aerosol composition

Abstract

Disclosed herein is an anti-pathogenic aerosol composition comprising an alcohol selected from ethanol and isopropanol; an alkyldimethylbenzylammonium compound; polyhexanide; and water.

Description

[Technical field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims the benefit of AU2022900472, the entire disclosure of which is incorporated herein by cross-reference.

[0002] Field of Disclosure

[0002] The present disclosure relates broadly to anti-pathogenic aerosol compositions and uses thereof. [Background technology]

[0003] Background of the disclosure

[0003] The discussion of prior art throughout this specification should in no way be considered as an admission that such prior art is widely known or forms part of the common general knowledge in the art.

[0004]

[0004] Pathogens such as bacteria and viruses pose a great threat to human health. Enclosed areas such as rooms provide a fertile environment for humans to become infected with infectious pathogens such as viruses. Therefore, effective cleaning / decontamination of enclosed and partially enclosed spaces is an important tool in the fight against the transmission of infectious pathogens. The importance of preventing the spread of such pathogens has been paramount in the fight against the Covid19 pandemic. Summary of the Invention [Problem to be solved by the invention]

[0005]

[0005] Against this background, the inventors have developed anti-pathogenic aerosol compositions and methods for dispersing them. [Means for solving the problem]

[0006] Disclosure Summary In a first aspect, a) an alcohol selected from ethanol and isopropanol; b) alkyldimethylbenzylammonium compounds; c) polyhexanide; and d) water An aerosol composition is provided comprising:

[0007]

[0007] The following options may be used in conjunction with the first aspect, either individually or in any combination.

[0008]

[0008] Alcohol may be present in the composition in an amount of about 0.5% to about 20% (v / v), or in an amount of about 1% to about 20% (v / v), or in an amount of about 1% to about 15% (v / v).

[0009]

[0009] The alkyldimethylbenzylammonium compound may be present in the composition in an amount of about 0.001% to about 5% (w / v), or in an amount of about 0.001% to about 1% (w / v), or in an amount of about 0.005% to about 1% (w / v), or in an amount of about 0.01% to about 1% (w / v), or in an amount of about 0.1% to about 1% (w / v), or in an amount of about 0.1% to about 0.8% (w / v), or in an amount of about 0.5% to about 1% (w / v).

[0010]

[0010] Polyhexanide may be present in the composition in an amount of about 0.005% to about 10% (w / v), or in an amount of about 0.005% to about 5% (w / v), or in an amount of about 0.05% to about 5% (w / v), or in an amount of about 0.05% to about 3% (w / v), or in an amount of about 0.5% to about 3% (w / v), or in an amount of about 0.5% to about 2.5% (w / v), or in an amount of about 1% to about 3% (w / v).

[0011]

[0011] Water may be present in the composition in an amount of about 10% to about 95% (v / v), or in an amount of about 15% to about 95% (v / v), or in an amount of about 15% to about 90% (v / v).

[0012] In one embodiment, the alcohol is ethanol.

[0013]

[0013] The alkyldimethylbenzylammonium compound has the following formula (I): [ka] where n is an integer from 6 to 20 and X is a halide.

[0014] In one embodiment, the alkyldimethylbenzylammonium compound is benzyldimethylhexadecylammonium chloride.

[0015]

[0015] The polyhexanide may have an average molecular weight of about 3000 Da to about 3600 Da.

[0016]

[0016] The composition may further comprise a propellant.

[0017] The propellant may be dimethyl ether.

[0018] The composition may be a liquid.

[0019] The composition may be a self-propellant composition.

[0020] The composition may be a single-phase composition.

[0021] The composition may be an anti-pathogenic composition.

[0022] The composition may be a virucidal composition.

[0023] The composition may be a bactericidal composition.

[0024]

[0024] The composition may be contained in a can.

[0025]

[0025] When applied, the composition may leave no or substantially no residue.

[0026]

[0026] The composition may contain the alkyldimethylbenzylammonium compound and polyhexanide as the sole active ingredient.

[0027]

[0027] The composition may contain no glycol compounds at all.

[0028]

[0028] In a second aspect, there is provided a method for killing or inactivating pathogens in a space, the method comprising dispersing a composition of the first aspect into the space as an aerosol.

[0029]

[0029] The following options may be used in conjunction with the second aspect, either individually or in any combination.

[0030]

[0030] The pathogen may be an airborne pathogen.

[0031]

[0031] Pathogens can be suspended in the air.

[0032]

[0032] Pathogens may be on surfaces within the space.

[0033]

[0033] Pathogens may be killed or inactivated in the air within the space.

[0034]

[0034] Pathogens may be killed or inactivated on surfaces within the space.

[0035]

[0035] The surface may be a horizontal surface.

[0036]

[0036] The pathogen may be a bacterium, a virus, a mold or a fungus.

[0037]

[0037] The pathogen may be a virus.

[0038] The virus may be an enveloped virus.

[0039]

[0039] The enveloped virus may be a coronavirus, a herpes virus or an influenza virus.

[0040]

[0040] The coronavirus may be 229E, NL63, OC43, HKU1, SARS-CoV-2, SARS-CoV or MERS-CoV.

[0041]

[0041] The bacterium can be E. coli, Legionella, Bacillus subtilis, or Staphylococcus aureus.

[0042]

[0042] The space may be an enclosed space or a partially enclosed space.

[0043] The aerosol may have a droplet size of less than 10 microns, or less than 5 microns.

[0044]

[0044] The aerosol can be dispersed by a nebulizer device.

[0045]

[0045] The aerosol can be dispensed from a spray can.

[0046] definition

[0046] Below are some definitions that may be helpful in understanding the description of the present disclosure. These are intended to be general definitions, and should in no way limit the scope of the present disclosure to only these terms, but are set forth for a better understanding of the following description.

[0047]

[0047] Throughout this specification, unless the context requires to the contrary, the word "comprise" or variations such as "comprises" or "comprising" will be understood to imply the inclusion of a stated element, integer or step or group of elements, integers or steps, but not the exclusion of other elements, integers or steps or groups of elements, integers or steps.

[0048]

[0048] The terms "a" and "an" are used herein to refer to one or to more than one (i.e., to at least one) of the grammatical object of the article. By way of example, "an element" means one element or more than one element.

[0049]

[0049] In the context of this specification, the term "about" is understood to refer to a range of numbers that one of ordinary skill in the art would consider equivalent to the recited value under the circumstances of achieving the same function or result.

[0050]

[0050] In the context of this specification, the term "aerosol composition" is understood to mean a composition that is suitable for dispensing as an aerosol. In other words, the term "aerosol composition" is synonymous with the term "composition that is suitable for dispersing as an aerosol."

[0051]

[0051] In the context of this specification, the term "halide" is understood to mean chloro, fluoro, bromo or iodo.

[0052]

[0052] Every numerical range recited herein is intended to include all subranges of the same numerical precision contained within the recited range. For example, a range of 1.0 to 5.0 is intended to include all subranges between (and including) the recited minimum of 1.0 and the recited maximum of 5.0, i.e., having a minimum value equal to or greater than 1.0 and a maximum value equal to or less than 5.0, such as 2.1 to 4.5. Every maximum numerical limitation recited herein is intended to include all lower numerical limitations contained therein, and every minimum numerical limitation recited herein is intended to include all higher numerical limitations contained therein. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0053] Detailed Description In one aspect of the present disclosure, a) an alcohol selected from ethanol and isopropanol; b) alkyldimethylbenzylammonium compounds; c) polyhexanide; and d) water An aerosol composition is provided comprising:

[0054] In one embodiment, the alkyldimethylbenzylammonium compound has the following formula (I): [ka] where n is an integer from 6 to 20, and X is a halide.

[0055] In another embodiment, n is an integer from 8 to 18. In a further embodiment, n is 8, 10, 12, 14, 16, or 18. In yet another embodiment, n is 16. In some embodiments, X is chloro, bromo, or iodo. In other embodiments, X is chloro. The composition may include multiple alkyldimethylbenzylammonium compounds of formula (I). For example, in one embodiment, the composition includes multiple compounds of formula (I) where n is 8, 10, 12, 14, 16, and 18. In a further embodiment, the alkyldimethylbenzylammonium compound is benzyldimethylhexadecylammonium chloride (also known as cetalkonium chloride).

[0056]

[0056] The alkyldimethylbenzylammonium compound may be present in the composition in an amount of about 0.001% to about 4% (w / v), or in an amount of about 0.005% to about 3% (w / v), or in an amount of about 0.01% to about 3% (w / v), or in an amount of about 0.01% to about 2% (w / v), or in an amount of about 0.01% to about 1% (w / v), or in an amount of about 0.001% to about 3% (w / v), or in an amount of about 0.001% to about 2% (w / v), or in an amount of about 0.001% to about 1% (w / v), or in an amount of about 0.02% to about 1% (w / v), or in an amount of about 0.02% to about 0.85% (w / v).

[0057]

[0057] The alkyl dimethyl benzyl ammonium compound may alternatively be present in the composition in an amount of about 0.001% to about 5% (w / v), or in an amount of about 0.001% to about 1% (w / v), or in an amount of about 0.005% to about 1% (w / v), or in an amount of about 0.01% to about 1% (w / v), or in an amount of about 0.1% to about 1% (w / v), or in an amount of about 0.1% to about 0.8% (w / v), or in an amount of about 0.5% to about 1% (w / v). The alkyl dimethyl benzyl ammonium compound may be present in the composition in an amount of about 0.1% to about 1% (w / v). The alkyl dimethyl benzyl ammonium compound may be present in the composition in an amount of about 0.6% (w / v).

[0058] Polyhexanide (also known as polyhexamethylene biguanide) has the following structure: [ka] has.

[0059]

[0059] In some embodiments, the polyhexanide has an average molecular weight of about 500 Da to about 50,000 Da, or an average molecular weight of about 1,000 Da to about 10,000 Da, or an average molecular weight of about 2,500 Da to about 4,000 Da, or an average molecular weight of about 3,000 Da to about 3,600 Da. The polyhexanide may have an average molecular weight of about 3,000 Da to about 3,600 Da.

[0060]

[0060] Polyhexanide may be present in the composition in an amount of about 0.001% to about 5% (w / v), or in an amount of about 0.001% to about 4% (w / v), or in an amount of about 0.001% to about 3% (w / v), or in an amount of about 0.005% to about 5% (w / v), or in an amount of about 0.005% to about 4% (w / v), or in an amount of about 0.005% to about 3% (w / v), or in an amount of about 0.01% to about 5% (w / v), or in an amount of about 0.01% to about 4% (w / v), or in an amount of about 0.01% to about 3% (w / v), or in an amount of about 0.02% to about 3% (w / v), or in an amount of about 0.02% to about 2.75% (w / v).

[0061]

[0061] Polyhexanide may alternatively be present in the composition in an amount of about 0.05% to about 5% (w / v), or in an amount of about 0.05% to about 3% (w / v), or in an amount of about 0.5% to about 3% (w / v), or in an amount of about 0.5% to about 2.5% (w / v), or in an amount of about 1% to about 3% (w / v). Polyhexanide may be present in the composition in an amount of about 1% to about 3% (w / v). Polyhexanide may be present in the composition in an amount of about 2% (w / v).

[0062]

[0062] Water may be present in the composition in an amount of about 10% to about 95% (v / v), or in an amount of about 10% to about 90% (v / v), or in an amount of about 15% to about 90% (v / v), or in an amount of about 18% to about 90% (v / v), or in an amount of about 30% to about 90% (v / v), or in an amount of about 50% to about 90% (v / v), or in an amount of about 70% to about 90% (v / v), or in an amount of about 80% to about 90% (v / v). Water may be present in the composition in an amount of about 80% to about 90% (v / v). Water may be present in the composition in an amount of about 85% to about 90% (v / v). Water may be present in the composition in an amount of about 90% (v / v).

[0063]

[0063] The alcohol may be present in the composition in an amount of about 0.5% to about 20% (v / v), or in an amount of about 1% to about 20% (v / v), or in an amount of about 10% to about 20% (v / v), or in an amount of about 1% to about 15% (v / v), or in an amount of about 2% to about 15% (v / v), or in an amount of about 2% to about 10% (v / v). The alcohol may be present in the composition in an amount of about 10% to about 20% (v / v). The alcohol may be present in the composition in an amount of about 10% to about 15% (v / v). The alcohol may be present in the composition in an amount of about 10% (v / v).

[0064]

[0064] In one embodiment, the composition further comprises a propellant.The propellant suitable for use in the composition includes, but is not limited to, liquefied petroleum gas (ethane, propane, ethylene, isobutane, n-butane, propylene, etc.), chlorofluorocarbons, fluorocarbons, nitrogen gas, carbon dioxide gas and dimethyl ether.In one embodiment, the propellant is dimethyl ether.

[0065]

[0065] The propellant may be present in the composition in an amount of about 60% to about 90% (v / v), or in an amount of about 65% to about 90% (v / v), or in an amount of about 65% to about 85% (v / v), or in an amount of about 65% to about 75% (v / v), or in an amount of about 65% to about 80% (v / v), or in an amount of about 70% to about 85% (v / v), or in an amount of about 75% to about 85% (v / v). The propellant may be present in the composition in an amount of about 60% to about 90% (v / v). The propellant may be present in the composition in an amount of about 70% (v / v).

[0066] In embodiments where the composition comprises a propellant, The alcohol may be present in the composition in an amount of about 0.5% to about 10% (v / v), or in an amount of about 0.5% to about 5% (v / v), or in an amount of about 2% to about 3% (v / v), or in an amount of about 1% to about 4% (v / v), or in an amount of about 1% to about 3% (v / v). The alcohol may be present in the composition in an amount of about 1% to about 4% (v / v). The alcohol may be present in the composition in an amount of about 3% to about 5% (v / v). The alcohol may be present in the composition in an amount of about 3% (v / v). The alkyl dimethyl benzyl ammonium compound may be present in the composition in an amount of about 0.001% to about 5% (w / v), or in an amount of about 0.001% to about 3% (w / v), or in an amount of about 0.005% to about 3% (w / v), or in an amount of about 0.01% to about 2% (w / v), or in an amount of about 0.01% to about 1% (w / v), or in an amount of about 0.1% to about 2% (w / v), or in an amount of about 0.1% to about 0.8% (w / v), or in an amount of about 0.5% to about 1% (w / v). The alkyl dimethyl benzyl ammonium compound may be present in the composition in an amount of about 0.1% to about 1% (w / v). The alkyl dimethyl benzyl ammonium compound may be present in the composition in an amount of about 0.6% (w / v). Polyhexanide may be present in the composition in an amount of about 0.005% to about 5% (w / v), or in an amount of about 0.01% to about 5% (w / v), or in an amount of about 0.01% to about 2% (w / v), or in an amount of about 0.05% to about 4% (w / v), or in an amount of about 0.5% to about 3.5% (w / v), or in an amount of about 1% to about 4% (w / v), or in an amount of about 0.5% to about 2.5% (w / v), or in an amount of about 1% to about 3% (w / v). Polyhexanide may be present in the composition in an amount of about 1% to about 3% (w / v). Polyhexanide may be present in the composition in an amount of about 2% (w / v). Water may be present in the composition in an amount of about 10% to about 35% (v / v), or in an amount of about 15% to about 35% (v / v), or in an amount of about 10% to about 30% (w / v), or in an amount of about 20% to about 30% (w / v), or in an amount of about 10% to about 25% (v / v), or in an amount of about 15% to about 20% (v / v). Water may be present in the composition in an amount of about 20% to about 30% (w / v). Water may be present in the composition in an amount of about 25% to about 27% (w / v). Water may be present in the composition in an amount of about 27% (w / v).

[0067]

[0067] The composition may further comprise one or more fragrances, such as natural fragrances. Natural fragrances include oils of natural origin, such as bergamot, bitter orange, lemon, mandarin, caraway, cedar leaf, clove leaf, cedarwood, geranium, lavender, orange, origanum, petitgrain, white cedar, patchouli, lavandin, neroli, rose absolute, and the like. Natural fragrances include extracts of flowers, stems and leaves, fruits, fruit skins, roots, woods, herbs and leaves, needles and branches, resins, and balsams. Other suitable fragrance oils are essential oils of relatively low volatility, which are mostly used as fragrance ingredients. Examples are sage oil, chamomile oil, clove oil, melissa oil, mint oil, cinnamon leaf oil, lime blossom oil, juniper berry oil, vetiver oil, olibanum oil, galbanum oil, ladanum oil, and lavandin oil. In some embodiments, the composition may further comprise cinnamaldehyde.

[0068] In some embodiments, the composition may include propylene glycol and / or potassium sorbate.

[0069]

[0069] In some embodiments, the composition may also include a corrosion inhibitor. When the composition is stored in a metal container, such as a metal can, the corrosion inhibitor prevents the metal from corroding. Suitable corrosion inhibitors include octadecyl diethanolamide, aminomethyl propanol benzoate, amine salts of mono- and di-mixed isooctyl phosphate dodecyl ester, ammonium benzoate, such as ammonium benzoate, dodecyl benzoate ammonium, and tetradecyl benzoate ammonium, butyl lauroyl sarcosinate, nitromethane, morpholine, hexamethyleneimine, piperidine, and ammonia. The composition may include a corrosion inhibitor that is ammonium benzoate. The corrosion inhibitor may be present in the composition in an amount of about 0.01 to about 1% (w / v), or about 0.01% to about 0.1%, about 0.01% to about 0.25%, about 0.01% to about 0.5%, about 0.01% to about 0.75%, about 0.1% to about 0.25%, about 0.1% to about 0.5%, about 0.1% to about 0.75%, about 0.1% to about 1%, about 0.25% to about 0.5%, about 0.1% to about 0.75%, about 0.1% to about 1%, about 0.5% to about 0.75%, about 0.5% to about 1%, or about 0.75% to about 1% (w / v). The corrosion inhibitor may be present in the composition in an amount of about 0.2% (w / v). The corrosion inhibitor may be ammonium benzoate present in the composition in an amount of about 0.2% (w / v). If the composition includes a propellant, the corrosion inhibitor may be present in the composition in an amount of about 0.01 to about 0.1% (w / v), or about 0.01% to about 0.05%, or about 0.05% to about 0.1%, or about 0.02 to about 0.07% (w / v). The corrosion inhibitor may be present in the composition in an amount of about 0.06% (w / v).

[0070] In some embodiments, the composition comprises alkyldimethylbenzylammonium compound and polyhexanide as a single active ingredient, i.e., antipathogenic ingredient.In the context of this specification, water and alcohol selected from ethanol and isopropanol are not considered to be active ingredients, i.e., antipathogenic ingredients.In this composition, water functions as a diluent and alcohol aids in aerosolization of the composition.

[0071]

[0071] In some embodiments, the composition does not contain any glycol compounds. A glycol compound is understood to be a compound having two hydroxyl groups attached to adjacent carbon atoms, such as propylene glycol and triethylene glycol.

[0072] In one embodiment, the composition comprises: a) ethanol in an amount of about 1% to about 5% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.05% to about 3% (w / v); c) polyhexanide in an amount of about 0.5% to about 5% (w / v); d) a propellant in an amount of about 65% to about 85% (v / v); and e) Water in an amount of about 10% to about 25% (v / v) Includes.

[0073] In another embodiment, the composition comprises: a) ethanol in an amount of about 1% to about 3.5% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.05% to about 2% (w / v); c) polyhexanide in an amount of about 0.5% to about 3% (w / v); d) a propellant in an amount of about 75% to about 85% (v / v); and e) Water in an amount of about 15% to about 20% (v / v) Includes.

[0074] In yet another embodiment, the composition comprises: a) ethanol in an amount of about 1% to about 3% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.1% to about 1% (w / v); c) polyhexanide in an amount of about 1% to about 3% (w / v); d) a propellant in an amount of about 75% to about 85% (v / v); and e) Water in an amount of about 16% to about 20% (v / v) Includes.

[0075] In yet a further embodiment, the composition comprises: a) ethanol in an amount of about 2% (v / v); b) benzyldimethylhexadecylammonium chloride in an amount of about 0.6% (w / v); c) polyhexanide in an amount of about 2% (w / v); d) a propellant in an amount of about 80% (v / v); and e) Water in an amount of about 18% (v / v) Includes.

[0076] In one embodiment, the composition comprises: a) ethanol in an amount of about 1% to about 5% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.05% to about 3.5% (w / v); c) polyhexanide in an amount of about 0.5% to about 5% (w / v); d) a propellant in an amount of about 60% to about 80% (v / v); and e) Water in an amount of about 10% to about 25% (v / v) Includes.

[0077] In another embodiment, the composition comprises: a) ethanol in an amount of about 1% to about 4% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.05% to about 2% (w / v); c) polyhexanide in an amount of about 0.5% to about 3.5% (w / v); d) a propellant in an amount of about 65% to about 80% (v / v); and e) Water in an amount of about 10% to about 20% (v / v) Includes.

[0078] In yet another embodiment, the composition comprises: a) ethanol in an amount of about 1% to about 3% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.1% to about 1.5% (w / v); c) polyhexanide in an amount of about 1% to about 3% (w / v); d) a propellant in an amount of about 68% to about 82% (v / v); and e) Water in an amount of about 16% to about 20% (v / v) Includes.

[0079] In yet a further embodiment, the composition comprises: a) ethanol in an amount of about 3% (v / v); b) benzyldimethylhexadecylammonium chloride in an amount of about 0.6% (w / v); c) polyhexanide in an amount of about 2% (w / v); d) a propellant in an amount of about 70% (v / v); and e) Water in an amount of about 27% (v / v) Includes.

[0080] In yet another embodiment, the composition comprises: a) ethanol in an amount of about 2% to about 3% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.03% to about 0.18% (w / v); c) polyhexanide in an amount of about 0.03% to about 0.6% (w / v); d) a propellant in an amount of about 70% to about 80% (v / v); and e) about 17% to about 27% (v / v) water Includes.

[0081] In one embodiment, the composition comprises: a) ethanol in an amount of about 1% to about 5% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.05% to about 3% (w / v); c) polyhexanide in an amount of about 0.5% to about 5% (w / v); d) a propellant in an amount of about 60% to about 85% (v / v); and e) Water in an amount of about 15% to about 35% (v / v) Includes.

[0082] In another embodiment, the composition comprises: a) ethanol in an amount of about 2% to about 4% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.05% to about 2% (w / v); c) polyhexanide in an amount of about 0.5% to about 3% (w / v); d) a propellant in an amount of about 65% to about 80% (v / v); and e) Water in an amount of about 15% to about 30% (v / v) Includes.

[0083] In yet another embodiment, the composition comprises: a) ethanol in an amount of about 2% to about 4% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.1% to about 1% (w / v); c) polyhexanide in an amount of about 1% to about 3% (w / v); d) a propellant in an amount of about 65% to about 75% (v / v); and e) Water in an amount of about 20% to about 30% (v / v) Includes.

[0084] In yet another embodiment, the composition comprises: a) ethanol in an amount of about 2.5% to about 3.5% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.1% to about 1% (w / v); c) polyhexanide in an amount of about 1.5% to about 2.5% (w / v); d) a propellant in an amount of about 65% to about 75% (v / v); and e) Water in an amount of about 20% to about 30% (v / v) Includes.

[0085] In yet another embodiment, the composition comprises: a) ethanol in an amount of about 2.5% to about 3.5% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.1% to about 1% (w / v); c) polyhexanide in an amount of about 1.75% to about 2.25% (w / v); d) a propellant in an amount of about 65% to about 75% (v / v); and e) about 25% to about 30% (v / v) water Includes.

[0086] In yet another embodiment, the composition comprises: a) ethanol in an amount of about 1% to about 4% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.01% to about 2% (w / v); c) polyhexanide in an amount of about 0.01% to about 3% (w / v); d) a propellant in an amount of about 65% to about 85% (v / v); and e) Water in an amount of about 15% to about 30% (v / v) Includes.

[0087] In yet another embodiment, the composition comprises: a) ethanol in an amount of about 1% to about 4% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.01% to about 2% (w / v); c) polyhexanide in an amount of about 0.01% to about 3% (w / v); d) a propellant in an amount of about 70% to about 80% (v / v); and e) Water in an amount of about 20% to about 30% (v / v) Includes.

[0088] In yet another embodiment, the composition comprises: a) ethanol in an amount of about 0.1% to about 6% (v / v); b) benzyldimethylhexadecyl ammonium chloride in an amount of about 0.05% to about 0.25% (w / v); c) polyhexanide in an amount of about 0.2% to about 1% (w / v); d) a propellant in an amount of about 60% to about 90% (v / v); e) ammonium benzoate in an amount of about 0.02% to about 0.08% (w / v); and f) Water in an amount of about 8% to about 36% (v / v) Includes.

[0089] In yet another embodiment, the composition comprises: a) ethanol in an amount of about 3% to about 4.5% (v / v); b) benzyldimethylhexadecylammonium chloride in an amount of about 0.18% (w / v); c) polyhexanide in an amount of about 0.6% (w / v); d) a propellant in an amount of about 70% (v / v); e) ammonium benzoate in an amount of about 0.06% (w / v); and f) about 25% to about 27% (v / v) water Includes.

[0090] In yet another embodiment, the composition comprises: a) an alcohol selected from ethanol and isopropanol; b) alkyldimethylbenzylammonium compounds; c) polyhexanide; and e) water It consists of:

[0091] In yet another embodiment, the composition comprises: a) an alcohol selected from ethanol and isopropanol; b) alkyldimethylbenzylammonium compounds; c) Polyhexanide; e) corrosion inhibitors; and e) water It consists of:

[0092] In yet another embodiment, the composition comprises: a) ethanol in an amount of about 10% to about 15% (w / v); b) benzyldimethylhexadecylammonium chloride in an amount of about 0.6% (w / v); c) polyhexanide in an amount of about 2% (w / v); e) ammonium benzoate in an amount of about 0.2% (w / v); and e) Water in an amount of about 85% to about 90% (w / v) It consists of:

[0093] In yet another embodiment, the composition comprises: a) an alcohol selected from ethanol and isopropanol; b) alkyldimethylbenzylammonium compounds; c) Polyhexanide; d) propellants; and e) water It consists of:

[0094] In yet another embodiment, the composition comprises: a) an alcohol selected from ethanol and isopropanol; b) alkyldimethylbenzylammonium compounds; c) Polyhexanide; d) propellant; e) corrosion inhibitors; and f) water It consists of:

[0095] In yet another embodiment, the composition comprises: a) an alcohol selected from ethanol and isopropanol; b) alkyldimethylbenzylammonium compounds; c) Polyhexanide; d) propellant; e) corrosion inhibitors; f) fragrances; and g) water It consists of:

[0096] In yet another embodiment, the composition comprises: a) an alcohol selected from ethanol and isopropanol; b) alkyldimethylbenzylammonium compounds; c) polyhexanide; and e) water Basically it consists of:

[0097] In yet another embodiment, the composition comprises: a) an alcohol selected from ethanol and isopropanol; b) alkyldimethylbenzylammonium compounds; c) Polyhexanide; e) corrosion inhibitors; and e) water Basically it consists of:

[0098] In yet another embodiment, the composition comprises: a) ethanol in an amount of about 10% to about 15% (w / v); b) benzyldimethylhexadecylammonium chloride in an amount of about 0.6% (w / v); c) polyhexanide in an amount of about 2% (w / v); e) ammonium benzoate in an amount of about 0.2% (w / v); and e) Water in an amount of about 85% to about 90% (w / v) Basically it consists of:

[0099] In yet another embodiment, the composition comprises: a) an alcohol selected from ethanol and isopropanol; b) alkyldimethylbenzylammonium compounds; c) Polyhexanide; d) propellants; and e) water Basically it consists of:

[0100] In yet another embodiment, the composition comprises: a) an alcohol selected from ethanol and isopropanol; b) alkyldimethylbenzylammonium compounds; c) Polyhexanide; d) propellant; e) corrosion inhibitors; and f) water Basically it consists of:

[0101] In yet another embodiment, the composition comprises: a) an alcohol selected from ethanol and isopropanol; b) alkyldimethylbenzylammonium compounds; c) Polyhexanide; d) propellant; e) corrosion inhibitors; f) fragrances; and g) water Basically it consists of:

[0102]

[0102] In the embodiments of paragraphs

[0072] to

[0101] above, the propellant may be dimethyl ether.

[0103]

[0103] In the embodiments of paragraphs

[0072] to

[0101] above, the amounts and properties of the alcohol, alkyldimethylbenzylammonium compound, and polyhexanide may be as described in each of the embodiments of paragraphs

[0053] to

[0071] .

[0104] In some embodiments, the aerosol may have a droplet size of less than 20 microns, or less than 18 microns, or less than 16 microns, or less than 14 microns, or less than 12 microns, or less than 10 microns, or less than 8 microns, or less than 6 microns, or less than 5 microns, or less than 4 microns, or less than 3 microns, or less than 2 microns, or from about 0.05 microns to about 10 microns, or from about 0.05 microns to about 8 microns, or from about 0.05 microns to about 6 microns, or from about 0.05 microns to about 5 microns, or from about 0.1 microns to about 5 microns, or from about 0.5 microns to about 5 microns, or from about 1 micron to about 5 microns, or from about 0.05 microns to about 4 microns, or from about 0.1 microns to about 4 microns, or from about 0.5 microns to about 4 microns, or from about 1 micron to about 4 microns, or from about 0.1 microns to about 3 microns, or from about 0.5 microns to about 3 microns, or from about 1 micron to about 3 microns. The droplet size can be an average droplet size.

[0105]

[0105] The composition can be prepared by methods well known to those skilled in the art. For example, a composition containing alcohol, alkyl dimethyl benzyl ammonium compound, polyhexanide and water can be prepared by mixing the desired amount of each component, followed by stirring until a homogeneous mixture is obtained. A composition containing a propellant can be prepared by adding the desired amount of alcohol, alkyl dimethyl benzyl ammonium compound, polyhexanide and water mixture (30% by volume) to a container, followed by adding propellant (70% by volume). The container is then sealed and fitted with a suitable dispenser suitable for dispersing the composition as an aerosol. Preferably, the container is a can.

[0106]

[0106] The composition is used in anti-pathogen applications, for example as a bactericide and / or a virucide.Accordingly, in another aspect of the present disclosure, a method for killing or inactivating pathogens in a space is provided, comprising dispersing the composition of the present disclosure in the space as an aerosol.

[0107]

[0107] Any standard form of commercial aerosol dispenser, including but not limited to aluminum and tin-plated steel containers, optionally including coatings and / or internal container linings, such as resin coatings, such as epoxy resin resins, may be utilized to dispense the composition. In one embodiment, the composition is dispensed using a spray can. For example, one or more spray cans may be placed at various locations in a room. The can is then actuated to release the aerosol composition. After a period of about 5-10 minutes, the contents of the can are dispensed into the room, and killing / inactivation of any pathogens present occurs. This method of dispensing is similar to the so-called "cockroach bombs" used to kill cockroaches in enclosed and partially enclosed spaces. The aerosol dispenser may contain about 150 mL to about 500 mL, or about 250 mL to about 500 mL of the composition.

[0108] Alternatively, the composition can be provided in a suitable form for dispersing using a spray device. Examples of spray devices include thermal spray, electric spray, pressurized air spray, ultrasonic spray and low energy vaporizer. In the embodiment where a spray device is used, no propellant is required in the composition. Preferably, the spray device is capable of dispersing the composition with aerosol droplet size of less than about 4 microns.

[0109]

[0109] The space in which pathogens can be killed / inactivated includes any and all spaces, whether enclosed or partially enclosed.Suitable spaces include, but are not limited to, rooms, transport containers, hospital wards, airplane interiors, car interiors, bus interiors, train interiors, homes, senior living facilities, gyms, ambulances, taxis, ride-sharing vehicles, corporate offices or meeting rooms, shopping malls, elevators, subway systems, or warehouses.Other suitable spaces will be recognized by those skilled in the art.

[0110] The method can be used to kill / inactivate pathogens including bacteria, viruses, molds and fungi. Surprisingly, the inventors have found that the composition can kill / inactivate airborne viruses (i.e., airborne, i.e., suspended in the air). That is, aerosolized particles of the composition suspended or floating in the air can contact and inactivate viruses that are also suspended or floating in the air. The composition can also kill / inactivate bacteria present on surfaces. Inactivating a virus is understood to mean that the virus is rendered incapable of infecting a host after contact with the composition of the present disclosure.

[0111]

[0111] In some embodiments, the virus is an enveloped virus, such as, for example, a coronavirus, a herpes virus, or an influenza virus.

[0112]

[0112] Coronaviruses constitute the subfamily Orthocoronavirinae. They are enveloped viruses with a single-stranded positive-sense RNA genome and a nucleocapsid with helical symmetry. Coronaviruses cause mild to fatal infections of the respiratory tract in humans. Non-limiting examples of coronaviruses include 229E, NL63, OC43, HKU1, SARS-CoV-2, SARS-CoV or MERS-CoV. Other coronaviruses will be apparent to those skilled in the art.

[0113] The influenza virus may be influenza A, B, C or D.

[0114]

[0114] The fungus may be Candida albicans or Aspergillus brasiliensis.

[0115]

[0115] Non-limiting examples of bacteria include E. coli, Legionella, Staphylococcus aureus, Salmonella typhimurium, Enterococcus hirae, Acinetobacter baumanii, Pseudomonas aeruginosa, Campylobacter jejuni, Bacillus subtillis, Listeria monocytogenes, and mycobacteria. EXAMPLES

[0116] Working Example Example 1

[0116] A composition comprising 85:15 water:ethanol containing 5% (w / v) polyhexanide (average molecular weight 3000-3600 Da) and 0.6% (w / v) benzyldimethylhexadecylammonium chloride prior to aerosolization was tested for its ability to prevent replication of MHV-1 in murine A9 ATCC / CCL 1.4 cells by using the protocol outlined in https: / / doi.org / 10.1016 / j.clae.2021.101513. Briefly, virus was added to a final concentration of 1 x 10 6 plaque forming units (PFU) / mL and incubated in the composition for 20 minutes.

[0117] A9 cells were cultured in 12-well tissue culture plates at 5-10×10 per well. 5Cells were seeded and allowed to adhere overnight at 37°C in 5% CO2. After incubation, the medium was removed and the cells were washed with 1X phosphate-buffered saline. Then, 100 μL of serially diluted virus in composition (in Dey Engley neutralizing broth; Becton Dickinson and Company, Macquarie Park, NSW, Australia) was inoculated into each well and incubated for 20 min at ambient temperature. After incubation, the cells were overlaid with 1% agarose (Sigma-Aldrich, Castle Hill, NSW, Australia) and DMEM solution (1:1) and further incubated for 72 h. After incubation, the cells were fixed with 4% formaldehyde for 1.5-2 h. The number of viral plaque-forming units was quantified after staining the cells with 1% crystal violet (Sigma-Aldrich). The reduction in PFU compared to the negative control (PBS) was calculated. The results are shown below.

[0118] [Table 1]

[0119] The test composition completely killed the SARS-CoV-2 coronavirus surrogate MHV-1, resulting in a viral load of at least 5 log 10 A decrease was given.

[0120] Example 2

[0118] Prior to aerosolization, the following compositions were tested for activity against Bacillus subtilis spores.

[0121] [Table 2]

[0122] B. subtilis spores were prepared by inoculating Bacillus subtilis ATCC 19659 in tryptic soy broth (TSB) and incubating overnight at 37°C for 12–16 h. After incubation, 100 mL of the bacterial suspension was plated on nutrient agar (NA; supplemented with 500 ppm MnSO4) and incubated at 37°C for 10–14 days to stimulate spore production. Spores were collected by washing the agar surface with sterile cold deionized water. After washing with sterile cold deionized water, the spores were stored in sterile deionized water at 4°C for further experiments.

[0123] To test the activity of Compositions A-D against B. subtilis spores, the spores were incubated at an OD of 0.102. 660 The spores were suspended in water until 100 μL. 100 μL of the spore suspension was added in duplicate to 100 μL of disinfectant solution. For the control, 100 μL of the spore suspension was added to 100 μL of water. These suspensions were then incubated for 20 minutes at ambient temperature (21° C.). After incubation, 100 μL of the solution was added to 900 μL of Dey Engley Neutralizing Broth (Oxoid, Basingstoke, UK) and serially diluted. Aliquots (20 μL) were then added to TSB agar plates and incubated at 35° C. for 24 hours. After incubation, the number of bacterial colonies was counted. The results are shown below.

[0124] [Table 3]

[0125]

[0121] Because B. subtilis spores are known to be highly resistant to disinfectants, the fact that the compositions are active against spores indicates that they may also be active against cells of other bacteria, such as staphylococci and Pseudomonas.

[0126] Example 3 A composition according to one embodiment of the present disclosure was tested for its ability to kill airborne coronavirus. The composition contained the following components: 0.6% w / v benzyldimethylhexadecyl ammonium chloride, 2% w / v polyhexanide (average molecular weight 3000-3600 Da), 70 mL dimethyl ether, 27 mL water, and 3 mL ethanol. This composition is hereinafter referred to as "Aerocide C19 Bomb."

[0127] Before starting the test, we evaluated several standards to see if they disclosed suitable protocols for testing the ability of aerosolized compositions to kill aerosolized viruses. We found ISO 16000:36 "Indoor air - Part 36: Standard method for assessing the reduction rate of culturable airborne bacteria by air purifiers using a test chamber" and EN17272:2020 "Chemical disinfectants and antiseptics - Methods of airborne room disinfection by automated process - Determination of bactericidal, mycobactericidal, sporicidal, fungicidal, yeasticidal, virucidal and phagocidal activities". However, neither standard disclosed suitable protocols.

[0128]

[0124] ISO 16000:36 discloses a method designed to evaluate the effectiveness of "a motorized device essentially made of a fan and a set of components capable of capturing and / or destroying (partially or completely) airborne pollutants." Therefore, it does not evaluate the activity of aerosolized disinfectants.

[0129]

[0125] EN17272:2020 discloses a method to "assess the disinfectant activity of airborne surface disinfection processes" in which microorganisms are dried onto stainless steel disks and then placed in a closed chamber in which the disks are placed. This standard evaluates aerosolized disinfectants, but is clear that it only evaluates their ability to reduce the number of microorganisms on surfaces and not the number of microorganisms in the air.

[0130]

[0126] As a result, a new protocol was developed that utilizes aspects of both ISO 16000:36 and EN17272:2020 to enable aerosolization of an anti-pathogenic composition and a microorganism and then capture of the microorganism after a period of time during which the anti-pathogenic composition and the microorganism are both present as an aerosol in a closed container.

[0131] Experimental protocol

[0127] Aerocide C19 Bomb was placed in a sealable 3m container to protect against airborne murine coronavirus ATCC / VR261 (MHV-1). 3 The experiments were performed in a chamber with ambient laboratory temperature and humidity set at 21° C. The virus was aerosolized using a Tekceleo nebulizer generating a 12 μm aerosol.

[0132] Cleaning of laboratory equipment

[0128] The working chamber and nebulizer were washed with 0.5% hydrogen peroxide, followed by 10 ml of 1× phosphate buffered saline (PBS) for 3 minutes to ensure that the equipment was clean and free of traces of hydrogen peroxide.

[0133] Mouse coronavirus For the control, a virus suspension (1×10 5Aerosolized plaque-forming units (PFUs) were aerosolized in a tent for 4 min. After 4 min, air vapour droplets were collected by a vacuum pump through Anderson sieves onto four glass petri dishes containing 2% w / v agar (BD, Macquarie Park, NSW, Australia). The Anderson sieve collects the largest (4.7 μm) sized aerosol at the top and the smallest (1.1 μm) at the bottom of the device.

[0134] For the test samples, an aerosol canister containing an Aerocide C19 Bomb (manufactured by Moorebank Aerosol) was activated in a sealed chamber for 4 min while a suspension of virus (1 × 10 5 Plaque forming units) were aerosolized in the tent for 4 minutes. After 4 minutes, the air vapor droplets were collected using the Anderson sieving apparatus as described above.

[0135]

[0131] Control and test samples were removed from the agar by scraping into 1500 μL of 20% w / v bovine serum albumin for each plate. A9 mouse fibroblast cells (ATCC / CCL 1.4), permissive for the growth of murine coronaviruses, were grown to confluence in DMEM containing 10% fetal bovine serum (FBS; Thermofisher), 100 μg / ml streptomycin sulfate, and 100 I.U. penicillin G, (Thermofisher). An aliquot (100 L) of the collected virus particles from each agar plate was inoculated into the wells of a 12-well plate containing A9 cells and incubated for 1 h at 37° C. in the presence of 5% (v / v) CO2. The plates were gently rocked once every 15 min to prevent the cells from drying out. After incubation, overlay medium containing a 50:50 mixture of 2% (w / v) agar (Sigma-Aldrich, Castle Hill, NSW, Australia) and DMEM was added to each well and further incubated for 72 h. After incubation, cells were fixed with 4% (v / v) formaldehyde (Sigma-Aldrich) for 2–3 h, the agar overlay was removed, and the number of plaques produced by viral particles (plaque-forming units; pfu) was visualized after staining with 1% (w / v) crystal violet (Sigma-Aldrich).

[0136] result

[0137] [Table 4]

[0138] conclusion Under test conditions, the Aerocide C19 Bomb reduced the number of aerosolized murine coronaviruses by 2 log 10 Therefore, the Aerocide C19 Bomb is effective in killing airborne coronaviruses.

[0139] Example 4

[0133] The Aerocide C19 Bomb used in Example 3 above was tested for its ability to kill bacteria.

[0140] The protocol followed that of the EN17272:2020 efficacy test, with the following modifications: 1. S. aureus ATCC 6538 and E. coli ATCC 10798 were used. 2. 0.3% bovine serum albumin (BSA) was used. 3. Glass slides were used 4.3m 3 A closed chamber was used. 5. No distribution test was performed 6. No preliminary tests (effectiveness tests and distribution tests) were conducted to verify the absence of residual effects. 7. The carriers were positioned horizontally and vertically in the test chamber. 8. Ramasol (Quin Global Pty Ltd) was used as a control.

[0141] Bacterial suspension Bacteria were grown first on tryptone soy agar (TSA) and then in tryptone soy broth (TSB). After overnight growth at 35° C., the bacteria were centrifuged and the OD 660 The turbidity was adjusted to 0.1 nm. The bacterial suspension was then further diluted with 0.3% BSA to give a final bacterial test suspension of 1 × 10 6 CFUml -1 Bacterial concentrations were confirmed by plating serial dilutions in D / E neutralizing fluid onto TSA.

[0142] test

[0136] The bacterial test suspension was utilized within 2 hours of preparation. 50 μL of the test suspension was spread onto a glass slide and allowed to dry for 45 minutes in a biosafety cabinet. Once the solution was dry, the glass slide was then removed using a 3 mm 3They were placed at the front and rear of the sealable chamber in horizontal and vertical positions at 50% or more of the tent height.

[0143]

[0137] The aerosol can containing the C19 Aerosol Bomb was activated and the chamber was closed while the Aerocide C19 Bomb was activated for 4 minutes. After 20 minutes, the tent was opened and the glass slide was retrieved.

[0144]

[0138] The glass slide was transferred to 10 mL of D / E Neutralizing Solution and 5 sterile glass beads were added. The tube was vortexed for 1 minute, then the solution was removed and serially diluted 10-fold into D / E Neutralizing Solution.

[0145]

[0139] An aliquot (100 mL) of the solution was then added to TSA and incubated for 24 hours at 35° C. After incubation, the number of bacterial colonies was counted.

[0146] result

[0140] The results are shown in Tables 1 and 2 below.

[0147] [Table 5]

[0148] [Table 6]

[0149] conclusion Under test conditions, Aerocide C19 Bomb provided good disinfectant activity against microorganisms on horizontal surfaces, achieving a 5 log reduction of EN 17272:2020. 10 Meet the ultra-low. Ramasol aerosols failed to meet the requirements of EN 17272:2020.

[0150]

[0142] Those skilled in the art will recognize that the disclosure described herein is susceptible to variations and modifications other than those specifically described. It should be understood that the present disclosure includes all such variations and modifications. The present disclosure also includes all of the steps, features, compositions and compounds referred to or shown herein, individually or collectively, and any and all combinations of two or more of the steps, features, compositions and compounds.

Claims

1. a) an alcohol selected from ethanol and isopropanol; b) alkyl dimethyl benzyl ammonium compounds; c) polyhexanide; and d) water 1. An aerosol composition comprising:

2. The composition of claim 1, wherein the alcohol is present in the composition in an amount of about 0.5% to about 20% (v / v).

3. 3. The composition of claim 1 or claim 2, wherein the alkyldimethylbenzylammonium compound is present in the composition in an amount of about 0.001% to about 5% (w / v).

4. The composition of any one of claims 1 to 3, wherein the polyhexanide is present in the composition in an amount of about 0.005% to about 10% (w / v).

5. The composition of any one of claims 1 to 4, wherein the water is present in the composition in an amount of about 10% to about 95% (v / v).

6. The composition according to any one of claims 1 to 5, wherein the alcohol is ethanol.

7. The alkyldimethylbenzylammonium compound has the following formula (I): 【Chemistry 1】 where n is an integer from 6 to 20 and X is a halide. The composition according to any one of claims 1 to 6, having the formula:

8. 8. The composition of claim 7, wherein the alkyl dimethyl benzyl ammonium compound is benzyl dimethyl hexadecyl ammonium chloride.

9. The composition of any one of claims 1 to 8, wherein the polyhexanide has an average molecular weight of about 3000 Da to about 3600 Da.

10. The composition of any one of claims 1 to 9, further comprising a propellant.

11. 11. The composition of claim 10, wherein the propellant is dimethyl ether.

12. The composition of any one of claims 1 to 9, further comprising a corrosion inhibitor.

13. The composition according to any one of claims 10 to 12, which is contained in a can.

14. A method for killing or inactivating pathogens in a space, the method comprising dispersing a composition according to any one of claims 1 to 13 as an aerosol into the space.

15. 15. The method of claim 14, wherein the pathogen is an airborne pathogen.

16. 15. The method of claim 14, wherein the pathogen is suspended in the air.

17. The method of claim 14 , wherein the pathogen is located on a surface.

18. The method according to any one of claims 14 to 17, wherein the pathogen is a bacterium, a virus, a mold or a fungus.

19. 20. The method of claim 18, wherein the pathogen is a virus.

20. 20. The method of claim 19, wherein the virus is an enveloped virus.

21. 21. The method of claim 20, wherein the enveloped virus is a coronavirus, a herpes virus, or an influenza virus.

22. 22. The method of claim 21 , wherein the enveloped virus is a coronavirus.

23. 23. The method of claim 22, wherein the coronavirus is 229E, NL63, OC43, HKU1, SARS-CoV-2, SARS-CoV or MERS-CoV.

24. 24. The method of claim 23, wherein the coronavirus is SARS-CoV-2.

25. 20. The method of claim 18, wherein the pathogen is a bacterium.

26. 26. The method of claim 25, wherein the bacterium is E. coli, Legionella, Staphylococcus aureus, Salmonella typhimurium, Enterococcus hirae, Acinetobacter baumanii, Pseudomonas aeruginosa, Campylobacter jejuni, Bacillus subtilis, Listeria monocytogenes, or mycobacteria.

27. The method according to any one of claims 14 to 26, wherein the space is an enclosed space or a partially enclosed space.

28. The method of any one of claims 14 to 27, wherein the aerosol has a droplet size of less than 10 microns, or less than 5 microns.

29. The method of any one of claims 14 to 28, wherein the aerosol is dispersed by a spray device.

30. The method of any one of claims 14 to 28, wherein the aerosol is dispensed from a spray can.

Images