Sunblock composition for protecting Lactobacillus bacteria and Staphylococcus epidermidis on the skin

Abstract

The present invention relates to the use of a selected UV filter selected from the group consisting of octocrylene, ethylhexyl triazone, and t-butyl methoxydibenzoylmethane for simultaneously protecting skin bacteria of the genus Lactobacillus, such as Lactobacillus crispatus (L. crispatus), and Staphylococcus epidermidis from UV radiation.

Description

Detailed Description of the Invention 【0001】 The present invention relates to the use of a selected UV filter selected from the group consisting of octocrylene, ethylhexyl triazone, and t-butyl methoxydibenzoylmethane for simultaneously protecting skin bacteria of the genus Lactobacillus, such as Lactobacillus crispatus (L. crispatus), and Staphylococcus epidermidis from UV radiation. 【0002】 Healthy skin is colonized by bacteria of the genus Lactobacillus, such as Lactobacillus crispatus (L. crispatus), of the family Lactobacillaceae. These are said to play a beneficial role by exerting a multifaceted local mechanism of action against pathogens and inflammation in the skin, which is their habitat. On the one hand, bacteria of the genus Lactobacillus have been shown to directly compete with skin pathogens by inhibiting adhesion, producing antibacterial metabolites, and affecting the metabolism of pathogens. The competitive anti-pathogenic effects of bacteria of the genus Lactobacillus with various common skin pathogens, such as Staphylococcus aureus, Cutibacterium acnes, and Candida albicans, have already been mechanistically explained. On the other hand, bacteria of the genus Lactobacillus also have an immunomodulatory capacity related to the suppression of excessive skin inflammation. The effects they exert on the immune system are mediated by bacterial metabolites and microbe-associated molecular patterns (MAMPs) that are associated with or secreted from the cell wall. In addition, bacteria of the genus Lactobacillus can also strengthen the skin barrier function when it is disrupted, for example, due to infection or inflammatory skin diseases. 【0003】 It is well known that UV light destroys microorganisms such as bacteria. 【0004】 Therefore, there is a continuing need to selectively protect beneficial bacteria, such as those of the genus Lactobacillus, from UV radiation. In addition, it is also desirable to protect Staphylococcus epidermidis. 【0005】 Accordingly, the inventors developed a targeted in vitro liquid model in 96-well microplate format for screening UV defense active substances ("UV filters") that allow bacteria to survive after UV irradiation. When the test was conducted in the presence of UV filter samples and their combinations, the effect of the selected UV irradiation conditions on the microbial population of Lactobacillus crispatus (L. crispatus) and Staphylococcus epidermidis (S. epidermidis) was evaluated using the change in the microbial population remaining after 4 hours. The inhibition of bacterial growth by UV irradiation was compared with the same strain not irradiated with UV and expressed as the survival rate (%). 【0006】 Surprisingly, it has been found here that certain UV filter substances are particularly suitable for protecting against the harmful effects of UV radiation, since they significantly and selectively improve the survival rate of bacteria of the genus Lactobacillus, in particular Lactobacillus crispatus (L. crispatus), and in addition Staphylococcus epidermidis (S. epidermidis) after UV light irradiation. 【0007】 Thus, in the first embodiment, the present invention relates to the use of one or more UV filters selected from the group consisting of octocrylene, t-butylmethoxydibenzoylmethane, and ethylhexyl triazone oxide for simultaneously protecting Lactobacillus bacteria, preferably Lactobacillus crispatus and Staphylococcus epidermidis, from the harmful effects of UV radiation. 【0008】 It is thought that the number of Lactobacillus bacteria and Staphylococcus epidermidis after exposure to UV radiation increases, i.e., the abundance of the bacteria increases, and thus the metabolites secreted by the bacteria also increase. 【0009】 The protection according to the present invention of skin bacteria of the genus Lactobacillus, particularly Lactobacillus crispatus, etc., after exposure to UV radiation (50 SED) is characterized in that the survival rate of bacteria of the genus Lactobacillus, particularly Lactobacillus crispatus, etc., is increased by at least 100% compared to the control. 【0010】 Similarly, Staphylococcus epidermidis is also simultaneously protected from UV radiation. The protection is characterized in that the survival rate of Staphylococcus epidermidis after exposure to UV radiation (15 SED) is increased by at least 20% compared to the control. 【0011】 Particularly preferred UV filters according to the present invention are octocrylene and a mixture of ethylhexyl triazone, butyl methoxy dibenzoylmethane, and bis-ethylhexyl oxy phenol methoxy phenyl triazine, because by using these, the survival rate of skin bacteria of the genus Lactobacillus, in particular Lactobacillus crispatus, etc., becomes particularly good, and at the same time, Staphylococcus epidermidis is also efficiently protected. 【0012】 In all embodiments of the present invention, the most preferred bacterium of the genus Lactobacillus is Lactobacillus crispatus CIP 103604. 【0013】 In all embodiments of the present invention, the most preferred Staphylococcus epidermidis is Staphylococcus epidermidis ATCC 12228. 【0014】 In all embodiments of the present invention, a particularly preferred bacterial species of the genus Lactobacillus is Lactobacillus crispatus. 【0015】 As used herein, the term UV radiation (used synonymously with UV light) refers to radiation in the UVA-UVB region of 280 to 400 nm. 【0016】 In all embodiments of the present invention, the amount of ethylhexyl triazone (also known as 2,4,6-tris[anilino(p-carbo-2'-ethyl-1'-hexyloxy)]-1,3,5-triazine (EHT)) used in accordance with the present invention is preferably selected from the range of 0.1% by weight to 10% by weight, more preferably from the range of 0.5% by weight to 7.5% by weight, and most preferably from the range of 1% by weight to 5% by weight. 【0017】 In all embodiments of the present invention, the amount of t-butyl methoxydibenzoylmethane (also known as avobenzone or 3-(4-tert-butylphenyl)-1-(4-methoxyphenyl)propane-1,3-dione (BMDBM)) used in accordance with the present invention is preferably selected from the range of 0.1% by weight to 10% by weight, more preferably from the range of 0.5% by weight to 7.5% by weight, and most preferably from the range of 1% by weight to 5% by weight. 【0018】 In all embodiments of the present invention, the amount of octocrylene (also known as 2-ethylhexyl 2-cyano-3,3-diphenylacrylate or ethylhexyl 2-cyano-3,3-diphenylacrylate (OC)) used in accordance with the present invention is preferably selected from the range of 0.1% by weight to 10% by weight, more preferably from the range of 0.5% by weight to 7.5% by weight, and most preferably from the range of 1% by weight to 5% by weight. 【0019】 In certain embodiments, a combination of octocrylene, ethylhexyl salicylate, bis-ethylhexyloxyphenol methoxyphenyl triazine, and t-butyl methoxydibenzoylmethane is used in a total amount that is more preferably in the range of 5 to 20% by weight, preferably in the range of 7.5 to 15, and most preferably in the range of 7.5 to 12% by weight. 【0020】 The UV filter is preferably contained in a suitable carrier such as a composition containing an oil selected from the group consisting of the following. 【0021】 The exact amount of the carrier will depend on the actual amounts of the UV filter and all other optional ingredients (e.g., other active ingredients) that a person skilled in the art would classify as different from the carrier. 【0022】 In a preferred embodiment, the sunscreen composition according to the invention comprises the carrier in an amount of 50% to 99% by weight, preferably 60% to 98% by weight, more preferably 70% to 98% by weight, particularly 80% to 95% by weight, etc., based on the total weight of the sunscreen composition. 【0023】 In a particular preferred embodiment, the carrier further consists of at least 40% by weight, more preferably at least 50% by weight, most preferably at least 55% by weight, particularly 55 to 90% by weight, etc., of water. 【0024】 In particular, the sunscreen composition according to the invention is a cosmetic composition or a pharmaceutical composition, preferably a cosmetic (non-therapeutic) composition. 【0025】 In one embodiment, the sunscreen composition according to the invention is applied in particular to the keratinous tissues of mammals such as human skin. 【0026】 As used in this application, the term "cosmetic composition" refers to the cosmetic compositions defined under the heading "Kosmetika" in Roempp Lexikon Chemie, 10th edition 1997, Georg Thieme Verlag Stuttgart, New York, and the cosmetic compositions disclosed in A. Domsch, "Cosmetic Compositions", Verlag fuer chemische Industrie (ed. H. Ziolkowsky), 4th edition, 1992. 【0027】 The sunscreen composition according to the present invention is an ultraviolet protection emulsion, an ultraviolet protection lotion, an ultraviolet protection cream, an ultraviolet protection oil, a sunblock, or a day care cream having a tropical or SPF (Sun Protection Factor), also known as a light protection preparation (sun care product, sunscreen). Particular interest has been focused on ultraviolet protection creams, ultraviolet protection lotions, ultraviolet protection emulsions, and ultraviolet protection preparations. 【0028】 The composition of the present invention (including the carrier) may contain conventional adjuvants and additives such as preservatives / antioxidants, fatty substances / oils, organic solvents, silicones, thickeners, softeners, emulsifiers, defoamers, aesthetic components such as fragrances, surfactants, fillers, anionic, cationic, non-ionic, or amphoteric polymers or mixtures thereof, propellants, acidifying or basifying agents, dyes, colorings / colorants, abrasives, absorbents, chelating agents and / or sequestering agents, essential oils, skin sensory stimulants, astringents, pigments, or any other optional components normally incorporated into this type of composition. 【0029】 According to the present invention, the composition according to the present invention may contain components for skin whitening, sunburn prevention, treatment of hyperpigmentation, acne, wrinkles, fine lines, atrophy, and / or prevention or reduction of inflammation, chelating agents and / or sequestering agents, anti-cellulite and slimming (e.g., phytic acid), firming, moisturizing and activating, self-tanning, calming, and agents for improving elasticity and skin barrier properties, and / or additional UV screening substances, and further components such as carriers and / or excipients or diluents conventionally used in sunscreen compositions. 【0030】 Unless otherwise stated, the following excipients, additives, diluents, etc. are suitable for the sunscreen composition according to the present invention. The required amounts of cosmetic and dermatological adjuvants and additives can be easily determined by those skilled in the art based on the desired product. 【0031】 Additional ingredients can be added to the oil phase, to the aqueous phase, or separately therefrom, when considered appropriate. The mode of addition can be readily adapted by a person skilled in the art. 【0032】 Examples of cosmetic excipients, diluents, adjuvants, additives, and active ingredients commonly used in the skin care industry that are suitable for use in the cosmetic composition of the present invention are described, for example, in the International Cosmetic Ingredient Dictionary & Handbook by the Personal Care Product Council (http: / / www.personalcarecouncil.org / ), which is viewable from online INFO BASE (http: / / online.personalcarecouncil.org / jsp / Home.jsp), but are not limited thereto. 【0033】 The cosmetic active ingredients useful herein can, in some cases, provide more than one benefit or act via more than one mechanism of action. 【0034】 Of course, a person skilled in the art will select the above-mentioned optional additional ingredients, adjuvants, diluents, and additives, and / or their amounts, taking care that the advantageous properties inherent in the combination according to the invention are not adversely affected or are not substantially adversely affected by one or more of the additions envisaged. 【0035】 The sunscreen composition according to the present invention can be in the form of a suspension or dispersion in a solvent or fatty substance, or in the form of an emulsion or microemulsion (in particular, oil-in-water (O / W) type, water-in-oil (W / O) type, silicone-in-water (Si / W) type or water-in-silicone (W / Si) type, PIT-emulsion, multiple emulsion (for example, oil-in-water-in-oil (O / W / O) type or water-in-oil-in-water (W / O / W) type), pickering emulsion, hydrogel, alcoholic gel, lipogel, single-phase or multi-phase solution, or vesicle dispersion), or can be applied by pen, as a beauty pack, or in other usual forms such as a spray. 【0036】 The sunscreen composition according to the present invention is preferably in the form of an oil-in-water (O / W) emulsion containing an oil phase dispersed in an aqueous phase in the presence of an O / W emulsifier. The preparation of such O / W emulsions is well known to those skilled in the art and is illustrated in the examples. 【0037】 In an advantageous embodiment, the O / W emulsifier is a phosphate ester emulsifier. Among the preferred phosphate ester emulsifiers, C 8~10 alkyl ethyl phosphate, C 9~15 alkyl phosphate, ceteareth-2 phosphate, ceteareth-5 phosphate, cetes-8 phosphate, cetes-10 phosphate, cetyl phosphate, (C6-10) pareth-4 phosphate (C 6-10 Pareth-4 Phosphate), (C12-15) pareth-2 phosphate, (C12-15) pareth-3 phosphate, ceteareth-2 phosphate DEA (DEA-Ceteareth-2 Phosphate), cetyl phosphate DEA, oleth-3 phosphate DEA, potassium cetyl phosphate, deceth-4 phosphate, deceth-6 phosphate, and trilauryl-4 phosphate may be mentioned. A specific phosphate ester emulsifier according to the present invention is, for example, potassium cetyl phosphate commercially available as Amphisol® K from DSM Nutritional Products Ltd Kaiseraugst. 【0038】 More suitable O / W emulsifiers according to the present invention include dipolyhydroxystearic acid PEG-30, dilauric acid PEG-4, dioleic acid PEG-8, oleic acid PEG-40 sorbitol, PEG-7 glyceryl cocoate, PEG-20 glyceryl almond fatty acid, PEG-25 hydrogenated castor oil, glyceryl stearate (and) stearic acid PEG-100, olive oil fatty acid PEG-7, oleic acid PEG-8, lauric acid PEG-8, PEG-60 glyceryl almond fatty acid, sesquistearic acid PEG-20 methylglucose, stearic acid PEG-40, stearic acid PEG-100, lauric acid PEG-80 sorbitan, stereth-2, stereth-12, oleth-2, ceteth-2, laureth-4, oleth-10, oleth 10 / polyoxyethylene (10) oleyl ether, ceteth-10, isostereth-20, ceteth-20, oleth-20, stereth-20, stereth-21, ceteth-20, isoceteth-20, laureth-23, stereth-100, glyceryl stearate citrate, glyceryl stearate (self-emulsifying type), stearic acid, salts of stearic acid, polyglyceryl-3 methylglucose distearate. Further suitable emulsifiers are sorbitan oleate, sorbitan sesquioleate, sorbitan isostearate, sorbitan trioleate, lauryl glucoside, decyl glucoside, sodium stearoyl glutamate, sucrose polystearate and hydrogenated polyisobutene. Furthermore, one or more synthetic polymers can be used as emulsifiers. For example, (VP / eicosene) copolymer, (acrylates / acrylic acid alkyl (C10-30)) cross-polymer, (acrylates / steareth-20 methacrylate) copolymer, (PEG-22 / dodecyl glycol) copolymer, (PEG-45 / dodecyl glycol) copolymer, and mixtures thereof. 【0039】 Another particularly suitable type of O / W emulsifier is, for example, an olive oil-derived nonionic self-emulsifying system known as (INCI name) cetearyl olive oil fatty acid and sorbitan olive oil fatty acid (chemical composition: sorbitan esters and cetearyl esters of olive oil fatty acids), sold under the trade name OLIVEM 1000. 【0040】 Furthermore, commercially available polymer emulsifiers, such as hydrophobically modified polyacrylic acids like (acrylates / alkyl acrylate (C10-30)) crosspolymer sold under the trade names Pemulen® TR-1 and TR-2 from Noveon, are also suitable. 【0041】 Another particularly suitable type of emulsifier is the polyglycerol ester or diester of fatty acids, also called polyglyceryl ester / diester (i.e., a polymer in which fatty acids are bonded by being esterified with polyglycerol), such as Isolan GPS commercially available from Evonik [INCI name (diisostearic acid / polyhydroxystearic acid / sebacic acid) polyglyceryl-4 (i.e., a diester of a mixture of isostearic acid, polyhydroxystearic acid, and sebacic acid and polyglyceryl-4)] or Dehymuls PGPH available from Cognis (INCI: polyglyceryl-2 dipolyhydroxystearate). 【0042】 Also, polyalkylene glycol ethers, such as Brij 72 (polyoxyethylene (2) stearyl ether) or Brij 721 (polyoxyethylene (21) stearyl ether) available from Croda, are similarly suitable. 【0043】 Particularly advantageous O / W emulsifiers according to the present invention are Polyglyceryl-3 Methyl Glucose Distearate, Lauryl Glucoside (and) Polyglyceryl-2 Dihydroxystearate, Glyceryl Sterate Citrate, Sodium Cetearyl Sulfate, Cetearyl Glucoside; Polyglyceryl-6 Stearate (and) Polyglyceryl-6 Behenate, Cetearyl Olive Oil Fatty Acid (and) Sorbitan Olive Oil Fatty Acid, Arachidyl Alcohol (and) Behenyl Alcohol (and) Arachidyl Glucoside, Cetearyl Alcohol (and) Coconut Alkyl Glucoside, Coconut Alkyl Glucoside (and) Coconut Alcohol, PEG-100 Stearate (and) Glyceryl Stearate, Sodium Stearoyl Glutamate, Steareth-20, Steareth-21, Steareth-25, Steareth-2, Ceteth-25, and Ceteth-6 (all described by their INCI names), one or more of them. 【0044】 The most preferred O / W emulsifiers according to the present invention are one or more of Ceteth-6 (optionally in a mixture with stearyl alcohol) and Ceteth-25. 【0045】 This at least one O / W or Si / W emulsifier is preferably used in an amount in the range of 0.5 to 10% by weight, particularly in the range of 0.5 to 5% by weight, most specifically in the range of 0.5 to 4% by weight, etc., based on the total weight of the composition. 【0046】 Suitable W / O or W / Si emulsifiers according to the present invention are polyglyceryl-2 dipolyhydroxystearate, PEG-30 dipolyhydroxystearate, cetyl dimethicone copolyol, polyglyceryl-3 diisostearate, polyglycerol ester of oleic acid / isostearic acid, polyglyceryl-6 ricinoleate, polyglyceryl-4 oleate, polyglyceryl-4 oleate / coconut oil fatty acid PEG-8-PG, magnesium stearate, sodium stearate, potassium laurate, potassium ricinoleate, sodium coconut fatty acid, sodium tallow fatty acid, potassium castor oil fatty acid, sodium oleate, and mixtures thereof. More suitable W / Si emulsifiers are lauryl polyglyceryl-3 polydimethylsiloxyethyl dimethicone, and / or PEG-9 polydimethylsiloxyethyl dimethicone, and / or cetyl PEG / PPG-10 / 1 dimethicone, and / or PEG-12 dimethicone cross polymer, and / or PEG / PPG-18 / 18 dimethicone. A particularly suitable W / O emulsifier for use in the compositions according to the present invention is PEG-30 dipolyhydroxystearate. At least one W / O emulsifier is preferably used in an amount of about 0.001 to 10% by weight, more preferably 0.2 to 7% by weight, based on the total weight of the composition. 【0047】 The sunscreen composition according to the present invention more preferably contains at least one surfactant auxiliary agent such as those selected from the group consisting of, for example, monoglycerides and diglycerides and / or fatty alcohols. The surfactant auxiliary agent is generally used in an amount selected from the range of 0.1 to 10% by weight, particularly in the range of 0.5 to 7% by weight, most particularly in the range of 1 to 5% by weight, based on the total weight of the composition. Particularly suitable surfactant auxiliary agents are selected from the list of alkyl alcohols such as cetyl alcohol (Lorol C16, Lanette 16), cetearyl alcohol (Lanette O), stearyl alcohol (Lanette 18), behenyl alcohol (Lanette 22), glyceryl stearate, glyceryl myristate (Estol 3650), hydrogenated coco-glyceryl (Lipocire Na10), and mixtures thereof. 【0048】 In a particularly advantageous embodiment according to the invention, the emulsifier is selected from the group consisting of cetearyl-6 and / or cetearyl-25, and the surfactant adjuvant is selected from the group consisting of behenyl alcohol, cetyl alcohol, cetearyl alcohol, and / or stearyl alcohol. 【0049】 The composition in the form of an O / W emulsion according to the invention can be provided, for example, in any formulation form of an O / W emulsion, such as in the form of a lotion, milk, or cream, which are prepared according to conventional methods. The composition which is the subject of the present invention is intended for topical application and can, in particular, constitute a dermatological or cosmetic composition which is intended, for example, to protect human skin from the adverse effects of UV radiation (wrinkle prevention, anti-aging, moisturizing, sun protection, etc.). 【0050】 According to an advantageous embodiment of the invention, the present composition constitutes a cosmetic composition and is intended for topical application to the skin. 【0051】 According to an advantageous aspect of the invention, the sunscreen composition according to the invention does not contain (i.e., is free of) 3-(4-methylbenzylidene) camphor and 2-hydroxy-4-methoxybenzophenone (INCI: oxybenzone). 【0052】 In another advantageous aspect of the invention, the sunscreen composition according to the invention further contains one or more of dibutyl adipate, dicaprylyl carbonate, stearyl alcohol, alkyl benzoate (C12-C15), caprylyl carbonate, tri(caprylic / capric acid) glyceryl, and mixtures thereof, preferably dibutyl adipate, stearyl adipate, and / or dicaprylyl carbonate. 【0053】 In another advantageous aspect of the invention, the sunscreen composition according to the invention further contains ethanol, more preferably in an amount of 0.5% to 10% by weight, more preferably 3% to 8% by weight, based on the total weight of the sunscreen composition. 【0054】 In a further advantageous embodiment of the present invention, the sunscreen composition of the present invention preferably further comprises a preservative and / or a preservative aid selected from the group consisting of phenoxyethanol, ethylhexylglycerin, hexylglycerin, glyceryl caprylate, caprylyl glycol, 1,2 - hexanediol, propanediol, propylene glycol, p - hydroxyacetophenone, and mixtures thereof, and most preferably selected from the group consisting of phenoxyethanol and ethylhexylglycerin and mixtures thereof. When the preservative or the preservative aid is present, they are preferably used in an amount of 0.01 to 2% by weight, more preferably in an amount of 0.05% to 1.5% by weight, most preferably in an amount of 0.1% to 1.0% by weight, for example, in an amount of 0.1% to 0.5% by weight, based on the total weight of the composition. 【0055】 In another advantageous embodiment, the sunscreen composition according to the present invention does not contain any of parabens, benzethonium chloride, piroctone olamine, lauroyl arginate, methylisothiazolinone, chloromethylisothiazolinone, bronopol, benzalkonium chloride, formaldehyde - releasing compounds, salicylic acid, triclosan, DMDM hydantoin, chlorphenesin, and IPBC (propargyl iodide butylcarbamate). 【0056】 In a further advantageous embodiment, the sunscreen composition of the present invention further comprises one or more of glycerin or polyhydroxystearic acid and mixtures thereof. 【0057】 In a further advantageous embodiment, the topical composition of the present invention further comprises a thickener, preferably a gum such as xanthan gum or Caesalpinia spinosa gum or a polyacrylate such as polyacrylate crosspolymer - 6 and mixtures thereof. 【0058】 The sunscreen composition according to the present invention generally has a pH in the range of 3 to 10, preferably in the range of 4 to 8, and most preferably in the range of 4 to 7. This pH can be easily adjusted as needed using appropriate acids such as citric acid or bases such as NaOH according to standard methods in the art. 【0059】 The topical composition according to the present invention can further contain one or more emollient agents that soothe and soften the skin. As an example, the emollient agent can be dicaprylyl carbonate or dibutyl adipate. Further emollient agents are silicone (dimethicone, cyclomethicone), vegetable oil (grape seed, sesame seed, jojoba, etc.), butter (cocoa butter, shea butter), alcohol (stearyl alcohol, cetyl alcohol), and petrolatum derivatives (petrolatum, mineral oil). 【0060】 In still another aspect, the sunscreen composition of the present invention can contain potassium cetyl phosphate as an emulsifier, and / or one or more of dibutyl adipate, dicaprylyl carbonate, and stearoyl alcohol. 【0061】 In still another aspect, the sunscreen composition of the present invention can contain acrylic esters such as (acrylates / acrylic acid alkyl (C10 - 30)) crosspolymer, and more preferably, it can be contained in an amount of 0.05 wt% to 1 wt%, more preferably 0.1 wt% to 0.5 wt% based on the total weight of the sunscreen composition. 【0062】 In other aspects, the sunscreen composition of the present invention can contain one or more fragrance substances selected from limonene, citral, linalool, α-isomethylionone, geraniol, citronellol, 2-isobutyl-4-hydroxy-4-methyltetrahydropyran, 2-tert-pentylcyclohexyl acetate, 3-methyl-5-phenyl-1-pentanol, 7-acetyl-1,1,3,4,4,6-hexamethyltetralin, adipic acid diester, cinnamal, amyl salicylate, α-amylcinnamaldehyde, α-methylionone, butylphenylmethylpropanal, cinnamal, amylcinnamyl alcohol, anis alcohol, benzoin, benzyl alcohol, benzyl benzoate, benzyl cinnamate, benzyl salicylate, bergamot oil, bitter orange oil, butylphenylmethylpropional, shouzuku seed oil, cedrol, cinnamal, cinnamyl alcohol, citronellyl methylcrotonate, citrus oil, coumarin, diethyl succinate, ethyl linalool, eugenol, Evernia furfuracea extract, moss extract, farnesol, guaiacwood oil, hexyl cinnamal, hexyl salicylate, hydroxycitronellal, lavender oil, lemon oil, linalyl acetate, mandarin orange oil, PCA menthyl, methylheptenone, nikuzuku kernel oil, rosemary oil, sweet orange oil, terpineol, tonka bean oil, triethyl citrate, vanillin. 【0063】 In other aspects, the sunscreen composition of the present invention can contain at least one salt of 2-phenylbenzimidazole-5-sulfonic acid. 【0064】 In other aspects, the composition can have an SPF of at least 20, preferably at least 30, for example, an SPF of 20 - 50 or 30 - 50. 【0065】 The sunscreen composition of the present invention can achieve the objective even when the total amount of the UV filter is surprisingly small. 【0066】 According to the present invention, advantageously, the sunscreen composition is present in the form of an emulsion or a dispersion, preferably in the form of an emulsion, more preferably in the form of an O / W emulsion. 【0067】 When the sunscreen composition of the present invention is in the form of an O / W emulsion, the formulation advantageously contains one or more O / W emulsifiers selected from glyceryl citrate stearate, glyceryl stearate (self-emulsifying type), stearic acid, stearate, polyglyceryl-3-methylglycose distearate, sodium cetearyl sulfate, potassium cetyl phosphate, polyglyceryl-10 stearate, and sodium stearyl glutamate. 【0068】 According to the present invention, advantageously, these O / W emulsifiers of the present invention can be present in the formulation at a concentration of 0.001 to 10% by weight, preferably 0.1 to 7% by weight, based on the total weight of the formulation. 【0069】 According to the present invention, preferably, the formulation contains potassium cetyl phosphate as an emulsifier. 【0070】 According to the present invention, more advantageously, the formulation contains cetyl alcohol, stearyl alcohol, and / or glyceryl stearate. 【0071】 According to the present invention, advantageously, the formulations of the present invention do not contain polyethylene glycol, polyethylene glycol ether, and polyethylene glycol ester (so-called PEG derivatives). 【0072】 The formulations of the present invention can advantageously contain a humectant. A humectant is a compound or a mixture of compounds that imparts to the cosmetic formulation the quality of reducing the water loss of the stratum corneum (also referred to as transepidermal water loss (TEWL)) and / or having a favorable effect on the hydration of the stratum corneum after the cosmetic formulation is applied or spread on the skin surface. 【0073】 Non-limiting examples of humectants advantageous for use in the present invention include glycerol, lactic acid and / or lactate, particularly sodium lactate, butylene glycol, propylene glycol, biosaccharide gum-1, azuki bean, ethylhexylglycerin, pyrrolidone carboxylic acid, and urea. In particular, it is more advantageous to use a polymer humectant from the group of polysaccharides that are water-soluble and / or water-swellable and / or gellable by the power of water. Particularly advantageous are, for example, hyaluronic acid, chitosan, and / or polysaccharides rich in fucose, and the polysaccharides rich in fucose are registered with the registration number 178463-23-5 in the Chemical Abstracts and are available, for example, from SOLABIA S.A. under the name Fucogel® 1000. The humectant can also be advantageously used, for example, as an anti-wrinkle active ingredient for protecting the skin from the types of changes caused by skin aging. 【0074】 The cosmetic preparation of the present invention may, although not essential, advantageously further contain, for example, a filler that further improves the functional and cosmetic properties of the preparation, for example, an effect that evokes or emphasizes the feeling of velvet or silk. Advantageous fillers in the meaning of the present invention are starch and starch derivatives (for example, tapioca starch, phosphate-crosslinked starch, aluminum or sodium octenyl succinate starch, etc.), pigments that essentially show neither a UV filtering effect nor a coloring effect (for example, boron nitride, etc.), and / or Aerosil® (CAS No. 7631-86-9) and / or talc and / or polyethylene, nylon, and dimethylsilylated silica. 【0075】 As an advantageous embodiment of the formulation of the present invention, embodiments in which the formulation contains one or more oils selected from dibutyl adipate, dicaprylyl carbonate, and / or alkyl (C12-15) benzoate, dibutyl sebacate, diisopropyl sebacate, dicaprylyl carbonate, dialkyl (C12,13) tartrate, butyloctyl salicylate, diethylhexyl malonate, hydrogenated castor oil dimer acid ester, triheptanoin, alkyl (C12,13) lactate, alkyl (C16,17) benzoate, propylheptyl caprylate, glyceryl tri(capryl / caprate), diethylhexyl naphthalenedicarboxylate, octyldodecanol, and ethylhexyl coconut oil fatty acid are also included. 【0076】 According to the present invention, formulations containing dibutyl adipate, dicaprylyl carbonate, and / or alkyl (C12-C15) benzoate are preferred. 【0077】 The aqueous phase of the formulation according to the invention advantageously contains conventional cosmetic adjuvants, for example, in each case, individually or in combination, alcohols, especially those with a low carbon number, preferably ethanol and / or isopropanol, or polyols with a low carbon number and their ethers, preferably propylene glycol, glycerol, electrolytes, self-tanning agents, and, in addition to these, especially one or more thickeners, advantageously thickeners that can be selected from the group of silicon dioxide, aluminum silicate, polysaccharides and / or their derivatives, such as hyaluronic acid, xanthan gum, hydroxypropylmethylcellulose, and particularly advantageously thickeners from the group of polyacrylates, preferably from the group called Carbopol (for example, Carbopol types 980, 981, 1382, 2984, and 5984). Further advantageous thickeners according to the invention include those with the INCI name (acrylates / alkyl acrylate (C10 - 30)) cross-polymer (for example, Pemulen TR 1, Pemulen TR 2, Carbopol 1328 from NOVEON), in addition to Aristoflex AVC (INCI: (acryloyldimethyltaurine ammonium / VP) copolymer) and Simugel NS (INCI: (hydroxyethyl acrylate / acryloyldimethyltaurine Na) copolymer (and) squalane (and) polysorbate 60). 【0078】 In this regard, according to the invention, it is preferred that the formulation contains xanthan gum, acrylate / alkyl acrylate (C10 - 30) cross-polymer, (hydroxyethyl acrylate / acryloyldimethyltaurine Na) copolymer (and) squalane (and) polysorbate 60, and / or vinyl pyrrolidone / hexadecene copolymer. 【0079】 According to the invention, it is particularly advantageous to contain at least 2.5% by weight of glycerol based on the total weight of the formulation. 【0080】 According to the present invention, it is advantageous for the formulation to contain one or more alkanediols from the group of 1,2-pentanediol, 1,2-hexanediol, 1,2-octanediol, 1,2-decanediol, 2-methyl-1,3-propanediol. 【0081】 According to the present invention, it is advantageous for the formulation of the present invention to contain ethanol, phenoxyethanol, and / or ethylhexylglycerin. 【0082】 According to the present invention, the formulation of the present invention preferably contains a film-forming agent. The film-forming agent in the sense of the present invention is various substances characterized by having the following properties: when the film-forming agent is dissolved in water or another suitable solvent and this solution is applied to the skin, the film-forming agent forms a film after the solvent has evaporated, and this film basically serves to fix the UV filter on the skin, thereby enhancing the SPF and / or water resistance of the product. 【0083】 It is particularly advantageous to select the film-forming agent from the group of polymers based on polyvinylpyrrolidone (PVP). 【0084】 For example, copolymers of vinylpyrrolidone such as (VP / hexadecene) copolymer and (VP / eicosene) copolymer available under the trade names Antaron V216 and Antaron V220 from GAF Chemicals Corporation are particularly preferred. 【0085】 Similarly, other polymer film formers are also advantageous, such as sodium polystyrene sulfonate available under the trade name Flexan 130 from National Starch and Chemical Corp. and / or polyisobutene available under the trade name Rewopal PIB1000 from Rewo. Examples of further suitable polymers are polyacrylamide (Seppigel 305), polyvinyl alcohol, PVP, PVP / VA copolymer, polyglycol, acrylate / octylacrylamide copolymer (Dermacryl 79). It is similarly advantageous to use hydrogenated castor oil dimer dilinoleate (CAS 646054-62-8, INCI: Hydrogenated Castor Oil Dimer Dilinoleate) available under the name Risocast DA-H from Kokyu Alcohol Kogyo Co., Ltd. or PPG-3 benzyl ether myristate (CAS 403517-45-3) available under the trade name Crodamol STS from Croda Chemicals. 【0086】 The following examples are provided to further illustrate the compositions and effects of the present invention. These examples are merely illustrative and are not intended to limit the scope of the present invention in any way. 【0087】 [Examples] [in vitro UV model] The microbial strains used in this test are listed below. The restoration of the cultures is carried out according to the instructions of CRBIP (Institut Pasteur Collection, France) or ATCC (American Type Culture Collection, USA), which are the collection centers of the reference strain suppliers: - Lactobacillus crispatus CIP 103604 - Staphylococcus epidermidis ATCC 12228 【0088】 A 96-well plate was filled with medium (140 μL), and the adjusted inoculum of each strain (20 μL) was added to achieve a final concentration of 1.106 colony-forming units (cfu / mL) per milliliter. After addition, the sample (40 μL; 20% v / v) was added in triplicate and thoroughly mixed to ensure uniform distribution. Subsequently, a UV load was applied to the plate using a UV irradiation device (BioSun, Vilber Lourmat, France) according to the UV irradiation doses shown in Table 1 below. This specific UV irradiation dose was selected such that the survival rate of the microbial population was between 10 and 49%. 【0089】 【Table 1】 【0090】 To count the number of microbial individuals, samples containing Lactobacillus crispatus (L. crispatus) and Staphylococcus epidermidis (S. epidermidis) were aliquoted and serially diluted (4 times 10-fold dilution followed by 8 times 2-fold dilution, in triplicate) in medium on a 96-well plate containing triphenyltetrazolium. Subsequently, considering the respiratory type of each strain, the plates were incubated at 37 °C ± 2.5 °C for 48 hours. After 48 hours under optimal growth conditions, the contamination rate was indicated from the last dilution showing pink color (triphenyltetrazolium) or turbidity, and it was possible to determine the reduction rate of the number of microbial individuals (the most probable number (MPN) method). In this experiment, a medium containing bacteria cultured without UV load was used as a control. The number of microbial individuals was expressed as cfu / mL and was taken as the average value of the experiments performed in triplicate. 【0091】 The use concentrations of single UV filters and their combinations in the test formulation were calculated such that the predicted in vitro SPF values were 5.75 + / - 0.15 and 23.45 + / - 0.65, respectively (Table 2). The UV filters were mixed in two oils, namely Tegosoft XC (55%) and Miglyol 829 Eco (the remaining amount up to 100%) and the emulsifier Tween 80 (5%). 【0092】 In the presence of test samples 1 to 10 at 20% v / v, the effect of the selected UV irradiation conditions on the survival of the microbial populations of Lactobacillus crispatus (L. crispatus) and Staphylococcus epidermidis (S. epidermidis) was compared with the corresponding strains (controls) not exposed to UV irradiation and without the presence of samples 1 to 10. The results after 4 hours of culture were compared with the number of viable microbial cells cultured without UV exposure (determination of the number of surviving microbial cells) and expressed as the survival rate. The relative protection rate (%) was calculated by dividing the difference in the survival rates of the samples and the solvent control (without UV irradiation) by the survival rate of the solvent control. The survival rates of each microbial cell number and the relative protection rate by the UV filter thus calculated are summarized in Table 2. 【0093】 [Table 2] 【0094】 [Table 3]

Claims

[Claim 1] The use of one or more UV filters selected from the group consisting of octocrylene, t-butyl methoxydibenzoylmethane, and ethylhexyl triazone oxide to simultaneously protect Lactobacillus bacteria and Staphylococcus epidermidis from the harmful effects of UV radiation. [Claim 2] The use according to claim 1, characterized in that the protection of Lactobacillus bacteria is characterized by a survival rate after exposure to UV radiation being increased by at least 100% compared to the control, and the protection of Staphylococcus epidermidis is characterized in that the survival rate after exposure to UV radiation is increased by 2% compared to the control. [Claim 3] The use according to claim 1 or 2, wherein the skin bacterium of the genus Lactobacillus is Lactobacillus crispatus. [Claim 4] The use according to claim 1 or 2, wherein the skin bacterium of the genus Lactobacillus is Lactobacillus crispatus CIP 103604. [Claim 5] The use according to claim 1 or 2, wherein the skin bacterium Staphylococcus epidermidis is Staphylococcus epidermidis ATCC 12228. [Claim 6] The use according to claim 1 or 2, wherein ethylhexyl triazone is used in an amount selected from the range of 0.1% to 10% by weight. [Claim 7] The use according to claim 1 or 2, wherein t-butylmethoxydibenzoylmethane is used in an amount selected from the range of 0.1% to 10% by weight. [Claim 8] The use according to claim 1 or 2, wherein octocrylene is used in an amount selected from the range of 0.1% by weight to 10% by weight.