Sunblock composition for protecting Lactobacillus bacteria and Staphylococcus epidermidis on the skin

Abstract

The present invention relates to the use of a selected UV filter selected from the group consisting of octocrylene, ethylhexyl triazone, and t-butyl methoxydibenzoylmethane to protect skin bacteria of the genus Lactobacillus, such as Lactobacillus crispatus (L. crispatus), from UV radiation, preferably in the co-presence of Staphylococcus epidermidis, in order to promote the maintenance of the health of the skin microbiome after UV irradiation.

Description

Detailed Description of the Invention 【0001】 The present invention relates to the use of a selected UV filter selected from the group consisting of octocrylene, ethylhexyl triazone, and t-butyl methoxydibenzoylmethane to protect skin bacteria of the genus Lactobacillus, such as Lactobacillus crispatus (L. crispatus), preferably in the coexistence of Staphylococcus epidermidis, in order to promote the maintenance of the health of the skin microbiome after UV irradiation. 【0002】 The skin is the outermost protective covering of an organism and the largest organ in the body. It serves as a barrier to protect the body from external factors. Furthermore, it is well known that a diverse collection of microorganisms that form the skin microbiome (often called the skin microbiota) is established on the surface of the skin. Most of these microorganisms are symbiotic organisms that establish a mutualistic relationship with the immune system of the skin. Dysbiosis of the skin microbiome is associated with many skin pathologies such as acne, atopic dermatitis, and psoriasis. 【0003】 Healthy skin is colonized by bacteria of the genus Lactobacillus, such as Lactobacillus crispatus (family Lactobacillaceae). These are said to play a beneficial role by exerting a multifaceted local mechanism of action against pathogens and inflammation in the skin, which is their habitat. On the one hand, Lactobacillus bacteria have been shown to directly compete with skin pathogens by inhibiting adhesion, producing antibacterial metabolites, and affecting the metabolism of pathogens. The competitive anti-pathogenic effects of Lactobacillus bacteria against various common skin pathogens, such as Staphylococcus aureus, Cutibacterium acnes, and Candida albicans, have already been mechanistically explained. On the other hand, Lactobacillus bacteria also have immunomodulatory capabilities related to the suppression of excessive skin inflammation. The effects they exert on the immune system are mediated by bacterial metabolites and microbe-associated molecular patterns (MAMPs) that are associated with or secreted from the cell wall. In addition, Lactobacillus bacteria can also strengthen the skin barrier function, for example, when it is damaged due to infectious or inflammatory skin diseases. 【0004】 It is well known that UV light destroys microorganisms such as bacteria. That is, UV radiation can induce significant changes in the skin microbiome, thereby potentially changing the immune response and / or causing damage to the skin barrier. 【0005】 Therefore, there is a continuing need to selectively protect beneficial bacteria, such as Lactobacillus bacteria, from UV radiation. In addition, it is also desirable to simultaneously protect Staphylococcus epidermidis, another beneficial skin commensal. 【0006】 Accordingly, the inventors developed a targeted in vitro liquid model in 96-well microplate format for screening UV-protective active substances ( "UV filters") that allow bacteria to survive even after UV irradiation. When the test was conducted in the presence of UV filter samples and their combinations, the effect of the selected UV irradiation conditions on the microbial counts of Lactobacillus crispatus (L. crispatus) and Staphylococcus epidermidis (S. epidermidis) was evaluated using the change in the microbial counts remaining after 4 hours. The inhibition of bacterial growth by UV irradiation was compared with the same strain without UV irradiation and expressed as the survival rate (%). Furthermore, a single-blind placebo-controlled clinical trial was conducted to specifically evaluate the effect of UV irradiation on the skin microbiome composition of human skin and the protection of the skin microbiome by a representative SFP20 sunscreen compared with a placebo formulation. 【0007】 Surprisingly, certain UV filter substances were found here to be particularly suitable for protecting against the harmful effects of UV radiation, as they significantly and selectively improve the survival rate of Lactobacillus bacteria on the skin, especially Lactobacillus crispatus (L. crispatus), more preferably even in the presence of Staphylococcus epidermidis (S. epidermidis). 【0008】 Accordingly, in a first embodiment, the present invention is a method for protecting bacteria of the genus Lactobacillus, preferably in the co - presence of Staphylococcus epidermidis, from the harmful effects of UV radiation, the method comprising applying, in an effective amount, a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, t - butyl methoxydibenzoylmethane, and ethylhexyl triazone to the outer surface of a human body having a skin microbiome containing said bacteria before exposing said outer surface to UV radiation. 【0009】 In other embodiments, the present invention relates to the use of one or more UV filters selected from the group consisting of octocrylene, t - butyl methoxydibenzoylmethane, and ethylhexyl triazone for protecting bacteria of the genus Lactobacillus, preferably in the co - presence of Staphylococcus epidermidis, from the harmful effects of UV radiation. 【0010】 In a further embodiment, the present invention is a method for increasing the difference in the abundance of bacteria of the genus Lactobacillus in the skin microbiome of an individual after exposure to UV radiation, preferably in the co - presence of Staphylococcus epidermidis, the method comprising topically administering, in an effective amount, a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, t - butyl methoxydibenzoylmethane, and ethylhexyl triazone to the outer surface of a body in need thereof before exposing said outer surface to UV radiation. 【0011】 In a further embodiment, the present invention also provides a method for providing protection to the outer surface of the human body by selectively increasing the abundance of skin bacteria of the genus Lactobacillus in the skin microbiome after exposure to UV radiation, preferably in the co - presence of Staphylococcus epidermidis, the method comprising the step of topically applying to the outer surface before exposure to UV radiation a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, t - butyl methoxydibenzoylmethane, and ethylhexyl triazone. 【0012】 In other embodiments, the present invention relates to the use of a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, t - butyl methoxydibenzoylmethane, and ethylhexyl triazone or one or more of said UV filters for selectively increasing the abundance of skin bacteria of the genus Lactobacillus in the skin microbiome of an individual in need thereof after exposure to UV radiation, preferably in the co - presence of Staphylococcus epidermidis. 【0013】 In other embodiments, the present invention relates to a composition comprising one or more UV filters selected from the group consisting of octocrylene, t - butyl methoxydibenzoylmethane, and ethylhexyl triazone for treating or preventing dysbiosis and / or strengthening the skin surface barrier after exposure to UV radiation. 【0014】 All methods and uses disclosed herein are particularly suitable for treating or preventing skin dysbiosis and / or strengthening the skin surface barrier after exposure to UV radiation. The compositions, methods, and uses disclosed herein are suitable for maintaining a healthy and balanced skin microbiome even after exposure to UV radiation. 【0015】 The number of bacteria belonging to the genus Lactobacillus increases after exposure to UV radiation, that is, the abundance of the bacteria increases, and it is considered that the metabolites secreted by the bacteria also increase. 【0016】 It is well understood by those skilled in the art that a composition containing a UV filter can also be referred to as a sunscreen composition. 【0017】 Although not wishing to be bound by theory, these metabolites are thought to contribute to the body's natural defense system. Thus, in another aspect, the present invention relates to the use of a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, t-butylmethoxydibenzoylmethane, and ethylhexyltriazone for improving the natural defense system of the human body surface by balancing the microbiome after exposing the surface to UV radiation. 【0018】 In a further aspect, the present invention also relates to a method for improving the natural defense system by balancing the microbiome of the human body surface, the method comprising applying a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, t-butylmethoxydibenzoylmethane, and ethylhexyltriazone to the surface before exposing it to UV radiation, and then exposing the surface to UV radiation, and optionally evaluating the beneficial effects resulting therefrom. 【0019】 Protection of skin bacteria of the genus Lactobacillus, particularly Lactobacillus crispatus, etc., after exposure to UV radiation (50 SED) according to the present invention is characterized in that the survival rate of skin bacteria of the genus Lactobacillus, particularly Lactobacillus crispatus, etc., is increased by at least 100% compared to the control. 【0020】 In a further advantageous embodiment, similarly Staphylococcus epidermidis is also simultaneously protected from UV radiation. Said protection is characterized in that the survival rate of Staphylococcus epidermidis after exposure to UV radiation (15 SED) is increased by at least 20% compared to the control. 【0021】 Particularly preferred UV filters according to the invention are octocrylene and a mixture of ethylhexyl triazone, t-butyl methoxydibenzoylmethane, and bis-ethylhexyloxyphenol methoxyphenyl triazine, because by using these, the survival rate of skin bacteria of the genus Lactobacillus, in particular Lactobacillus crispatus, becomes particularly good. 【0022】 In all embodiments of the present invention, the most preferred bacterium of the genus Lactobacillus is Lactobacillus crispatus CIP 103604. 【0023】 In all embodiments of the present invention, the most preferred Staphylococcus epidermidis is Staphylococcus epidermidis ATCC 12228. 【0024】 The total amount of the UV filter in the sunscreen composition greatly depends on the targeted protection and the UV radiation to which it is exposed. Preferably, in all embodiments of the present invention, the amount of the UV filter is selected such that the SPF of the sunscreen composition is in the range of at least 20, preferably at least 25, most preferably at least 30, for example, 20 to 100, 20 to 50, 25 to 100, 25 to 50, 30 to 100 or 30 to 50. 【0025】 A sunscreen composition with an SPF of 20 contains, for example, a UV filter in a total amount of preferably 10 to 40% by weight, more preferably 15 to 25% by weight, based on the total weight of the composition. 【0026】 A sunscreen composition with an SPF of 50 contains, for example, a UV filter in a total amount of preferably 15 to 50% by weight, more preferably 20 to 40% by weight, based on the total weight of the composition. 【0027】 In all embodiments of the present invention, a particularly preferred bacterial species of the genus Lactobacillus is Lactobacillus crispatus. 【0028】 As used herein, the term UV radiation (used synonymously with UV light) refers to radiation in the UVA - UVB region of 280 - 400 nm. 【0029】 As used herein, the term "outer surface of the human body" refers to the skin and the scalp. Preferably, in all embodiments of the present invention, the outer surface of the human body treated according to the present invention is the skin of the face, neck, and / or body. 【0030】 As used herein, the term "skin microbiome" refers to the microbial community that has colonized a defined skin area of an individual, such as, but not limited to, the forehead, cheeks, or scalp. 【0031】 As used herein, the term "difference in abundance" refers to the logarithm of the fold change between two conditions of the abundance of a taxon. This analytical method, which gives the difference as an output, makes it possible to identify the taxa of the microbiome associated with a specific biological state or clinical symptom. The differences can be ranked and sorted in ascending order. This "ranking" provides information about the relative association between a given covariate (i.e., treatment or time) and a feature. 【0032】 As used herein, the "difference in abundance" is determined by using software called Songbird, as outlined in Morton et al. (Nat. Commun., 2019. 10(1): p. 2719), which is incorporated herein by reference. This enables the construction of a statistical model for testing differences between treatments and calculating the differences starting from relative abundance data obtained from 16S rRNA sequencing. The output is a file containing the ranking of features for a particular metadata category. The higher the rank, the higher the relevance to that category (i.e., treatment). 【0033】 As used herein, the term "increase in difference in abundance" is defined as an increase in the difference in abundance when compared to the difference in abundance of a particular microorganism on each symmetrical skin region treated with placebo in the same individual. 【0034】 In all embodiments of the present invention, the amount of ethylhexyl triazone (also known as 2,4,6-tris[anilino(p-carbo-2'-ethyl-1'-hexyloxy)]-1,3,5-triazine (EHT)) in the sunscreen composition according to the present invention is preferably selected from the range of 0.1% by weight to 10% by weight, more preferably from the range of 0.5% by weight to 7.5% by weight, and most preferably from the range of 1% by weight to 5% by weight, based on the total weight of the sunscreen composition. Even more preferred ranges are 1 - 5% by weight or 1.5 - 4% by weight or 2 - 3% by weight. 【0035】 In all embodiments of the present invention, the amount of t-butyl methoxydibenzoylmethane (also known as avobenzone or 3-(4-tert-butylphenyl)-1-(4-methoxyphenyl)propane-1,3-dione (BMDBM)) in the sunscreen composition according to the present invention is preferably in the range of 0.1% to 10% by weight, more preferably in the range of 0.5% to 7.5% by weight, and most preferably in the range of 1% to 5% by weight, based on the total weight of the sunscreen composition. Even more preferred ranges are 2 to 7.5% by weight or 3 to 7% by weight or 4 to 6% by weight. 【0036】 In all embodiments of the present invention, the amount of octocrylene (also known as 2-ethylhexyl 2-cyano-3,3-diphenylacrylate or ethylhexyl 2-cyano-3,3-diphenylacrylate (OC)) in the sunscreen composition according to the present invention is preferably in the range of 0.1% to 10% by weight, more preferably in the range of 0.5% to 7.5% by weight, and most preferably in the range of 1% to 5% by weight, based on the total weight of the sunscreen composition. Even more preferred ranges are 2 to 7.5% by weight or 3 to 7% by weight or 4 to 6% by weight. 【0037】 In certain embodiments, the sunscreen composition according to the present invention comprises octocrylene, ethylhexyl salicylate, bis-ethylhexyloxyphenol methoxyphenyl triazine, and t-butyl methoxydibenzoylmethane, and more preferably comprises them in an amount of 15 to 20% by weight based on the total weight of the sunscreen composition. Such compositions are particularly suitable for increasing the difference in the abundance of bacteria of the genus Lactobacillus, particularly Lactobacillus crispatus, in the skin microbiome of an individual after UV radiation exposure. 【0038】 When a combination of ethylhexyl triazone, t-butyl methoxydibenzoylmethane, and bis-ethylhexyloxyphenol methoxyphenyl triazine is used, the amount of each of the UV filters is preferably selected from the range of 1 to 5% by weight, more preferably from the range of 2 to 4% by weight, and most preferably from the range of about 3% by weight, based on the total weight of the composition. 【0039】 The UV filters used according to the present invention are preferably included in a composition containing one or more oils suitable for dissolving and / or diluting the UV filters in the amounts indicated herein. Such oils are well known in the art. Particularly suitable oils are selected from the group consisting of 2-phenoxyethyl octanoate (also known as Tegosoft® XC) and triglyceride (caprylic acid / capric acid / succinic acid) (also known as Miglyol® 829 Eco). The composition may further preferably contain an emulsifier such as polysorbate-80 (polyoxyethylene (80) sorbitan monooleate, also known as Tween® 80). 【0040】 In all embodiments of the present invention, preferably, the total amount of oil in the composition according to the present invention is preferably selected from the range of 50 to 95% by weight, more preferably from the range of 50 to 90% by weight, and most preferably from the range of 75 to 90% by weight, based on the total weight of the composition. 【0041】 In all embodiments of the present invention, preferably, the total amount of emulsifier in the composition according to the present invention is preferably selected from the range of 1 to 7.5% by weight, more preferably from the range of 2.5 to 7.5% by weight, and most preferably from the range of 3 to 6% by weight, based on the total weight of the composition. 【0042】 Since the sunscreen composition according to the present invention is intended for topical application, it is well understood that the sunscreen composition according to the present invention contains a physiologically acceptable medium, that is, a medium compatible with keratinous substances such as the skin, mucous membranes, and keratin fibers. In particular, the physiologically acceptable medium is a cosmetically acceptable carrier. 【0043】 As used herein, the term "cosmetically acceptable carrier" refers to all carriers and / or excipients and / or diluents conventionally used in topical cosmetic compositions such as skin care preparations. 【0044】 The exact amount of the carrier will depend on the actual amounts of the UV filter and all other optional components (e.g., other active ingredients) that one of ordinary skill in the art would classify as different from the carrier. 【0045】 In an advantageous embodiment, the sunscreen composition according to the present invention contains the carrier in an amount of 50% to 99% by weight, preferably 60% to 98% by weight, more preferably 70% to 98% by weight, particularly 80% to 95% by weight, etc., based on the total weight of the sunscreen composition. 【0046】 In a particular advantageous embodiment, the carrier further consists of at least 40% by weight, more preferably at least 50% by weight, most preferably at least 55% by weight, particularly 55 to 90% by weight, etc., of water. 【0047】 In particular, the sunscreen composition according to the present invention is a cosmetic composition or a pharmaceutical composition, preferably a cosmetic (non-therapeutic) composition. 【0048】 In one embodiment, the sunscreen composition according to the present invention is applied particularly to keratinous tissues of mammals such as human skin. 【0049】 As used herein, the term "cosmetic composition" refers to cosmetic compositions as defined under the heading "Kosmetika" in Roempp Lexikon Chemie, 10th edition 1997, Georg Thieme Verlag Stuttgart, New York, and cosmetic compositions disclosed in A. Domsch, "Cosmetic Compositions", Verlag fuer chemische Industrie (ed. H. Ziolkowsky), 4th edition, 1992. 【0050】 The sunscreen composition according to the present invention is an ultraviolet protection emulsion, an ultraviolet protection lotion, an ultraviolet protection cream, an ultraviolet protection oil, a sunblock, or a day care cream having a tropical or SPF (Sun Protection Factor), also known as a photoprotective preparation (sun care product, sunscreen). Particular interest lies in ultraviolet protection creams, ultraviolet protection lotions, ultraviolet protection emulsions, and ultraviolet protection preparations. 【0051】 The composition of the present invention (including the carrier) may contain conventional auxiliaries and additives such as preservatives / antioxidants, fatty substances / oils, organic solvents, silicones, thickeners, softeners, emulsifiers, defoamers, aesthetic components such as fragrances, surfactants, fillers, anionic, cationic, nonionic, or amphoteric polymers or mixtures thereof, propellants, acidifying or basifying agents, dyes, colorings / colorants, abrasives, absorbents, chelating agents and / or sequestering agents, essential oils, skin sensation stimulants, astringents, pigments, or any other components normally incorporated into this type of composition. 【0052】 According to the present invention, the composition according to the present invention comprises components for skin whitening, sunburn prevention, treatment of hyperpigmentation, acne, wrinkles, fine lines, atrophy and / or prevention or reduction of inflammation, chelating agents and / or sequestering agents, anti-cellulite and slimming (e.g., phytic acid), tightening, moisturizing and revitalizing, self-tanning, calming, and agents for improving elasticity and skin barrier properties, and / or additional UV screening substances, and / or further components such as carriers and / or excipients or diluents conventionally used in sunscreen compositions. 【0053】 Unless otherwise specified, the following excipients, additives, diluents, etc. are suitable for the sunscreen composition according to the present invention. The required amounts of cosmetic and dermatological adjuvants and additives can be readily determined by those skilled in the art based on the desired product. 【0054】 Additional components can be added to the oily phase, the aqueous phase, or separately therefrom if considered appropriate. The mode of addition can be readily adapted by those skilled in the art. 【0055】 Examples of cosmetic excipients, diluents, adjuvants, additives, and active ingredients commonly used in the skin care industry and suitable for use in the cosmetic composition of the present invention are described, for example, in the International Cosmetic Ingredient Dictionary & Handbook by the Personal Care Product Council (http: / / www.personalcarecouncil.org / ), which is viewable from online INFO BASE (http: / / online.personalcarecouncil.org / jsp / Home.jsp), but are not limited thereto. 【0056】 The cosmetic active ingredients useful herein can, in some cases, provide more than one benefit or act via more than one mechanism of action. 【0057】 Of course, those skilled in the art will select the above-mentioned optional additional components, adjuvants, diluents, and additives, and / or their amounts, while taking care that the advantageous properties inherent in the combination according to the invention are not adversely affected or are substantially not adversely affected by the addition of one or more of the envisaged additives. 【0058】 The sunscreen composition according to the invention can be in the form of a suspension or dispersion in a solvent or fatty substance, or in the form of an emulsion or microemulsion (in particular, oil-in-water (O / W) type or water-in-oil (W / O) type, silicone-in-water (Si / W) type or water-in-silicone (W / Si) type, PIT-emulsion, multiple emulsion (for example, oil-in-water-in-oil (O / W / O) type or water-in-oil-in-water (W / O / W) type), pickering emulsion, hydrogel, alcoholic gel, lipogel, single-phase or multiphase solution, or vesicle dispersion), or in other conventional forms that can also be applied by pen, as a beauty pack, or as a spray. 【0059】 The sunscreen composition according to the invention is preferably in the form of an oil-in-water (O / W) emulsion comprising an oil phase dispersed in an aqueous phase in the presence of an O / W emulsifier. The preparation of such O / W emulsions is well known to those skilled in the art and is illustrated in the examples. 【0060】 In an advantageous embodiment, the O / W emulsifier is a phosphate ester emulsifier. Among the preferred phosphate ester emulsifiers, C 8~10 alkyl ethyl phosphate, C 9~15 alkyl phosphate, cetareth-2 phosphate, cetareth-5 phosphate, cetes-8 phosphate, cetes-10 phosphate, cetyl phosphate, (C6-10) pareth-4 phosphate (C 6-10(C12-15) Pareth-4 Phosphate, (C12-15) Pareth-2 Phosphate, (C12-15) Pareth-3 Phosphate, DEA-Ceteareth-2 Phosphate, DEA Cetyl Phosphate, DEA Oleth-3 Phosphate, Potassium Cetyl Phosphate, Deceth-4 Phosphate, Deceth-6 Phosphate, and Trilauryl-4 Phosphate. Specific phosphate ester emulsifiers according to the present invention are, for example, Potassium Cetyl Phosphate commercially available as Amphisol® K from DSM Nutritional Products Ltd Kaiseraugst. 【0061】 More suitable O / W emulsifiers according to the present invention include dipolyhydroxystearic acid PEG-30, dilauric acid PEG-4, dioleic acid PEG-8, oleic acid PEG-40 sorbitol, PEG-7 glyceryl cocoate, PEG-20 glyceryl almond fatty acid, PEG-25 hydrogenated castor oil, glyceryl stearate (and) stearic acid PEG-100, olive oil fatty acid PEG-7, oleic acid PEG-8, lauric acid PEG-8, PEG-60 glyceryl almond fatty acid, sesquistearic acid PEG-20 methylglucose, stearic acid PEG-40, stearic acid PEG-100, lauric acid PEG-80 sorbitan, steareth-2, steareth-12, oleth-2, ceteth-2, laureth-4, oleth-10, oleth 10 / polyoxyethylene (10) oleyl ether, ceteth-10, isosteareth-20, ceteth-20, oleth-20, steareth-20, steareth-21, ceteth-20, isoceteth-20, laureth-23, steareth-100, glyceryl stearate citrate, glyceryl stearate (self-emulsifying type), stearic acid, salts of stearic acid, polyglyceryl-3 methylglucose distearate. Further suitable emulsifiers are sorbitan oleate, sesquioleic acid sorbitan, sorbitan isostearate, sorbitan trioleate, lauryl glucoside, decyl glucoside, sodium stearoyl glutamate, sucrose polystearate, and hydrogenated polyisobutene. Furthermore, one or more synthetic polymers can be used as emulsifiers. For example, (VP / eicosene) copolymer, (acrylates / acrylic acid alkyl (C10-30)) cross-polymer, (acrylates / steareth-20 methacrylate) copolymer, (PEG-22 / dodecyl glycol) copolymer, (PEG-45 / dodecyl glycol) copolymer, and mixtures thereof. 【0062】 Another particularly suitable type of O / W emulsifier is, for example, the non-ionic self-emulsifying system derived from olive oil, known as cetearyl olive oil fatty acid and sorbitan olive oil fatty acid (chemical composition: sorbitan esters and cetearyl esters of olive oil fatty acids), sold under the trade name OLIVEM 1000. 【0063】 Furthermore, commercially available polymer emulsifiers, such as hydrophobically modified polyacrylic acids like (acrylates / alkyl acrylates (C10-30)) crosspolymer sold under the trade names Pemulen® TR-1 and TR-2 from Noveon, are also suitable. 【0064】 Another particularly suitable type of emulsifier is the polyglycerol ester or diester of fatty acids, also called polyglyceryl ester / diester (i.e., a polymer in which fatty acids are bonded by being esterified with polyglycerol), such as Isolan GPS commercially available from Evonik [INCI name (diisostearic acid / polyhydroxystearic acid / sebacic acid) polyglyceryl-4 (i.e., a diester of a mixture of isostearic acid, polyhydroxystearic acid, and sebacic acid and polyglyceryl-4)] or Dehymuls PGPH available from Cognis (INCI: polyglyceryl-2 dipolyhydroxystearate). 【0065】 Also, polyalkylene glycol ethers, such as Brij 72 (polyoxyethylene (2) stearyl ether) or Brij 721 (polyoxyethylene (21) stearyl ether) available from Croda, are similarly suitable. 【0066】 Particularly advantageous O / W emulsifiers according to the invention are one or more of Polyglyceryl-3 Methylglucose Distearate, Lauryl Glucoside (and) Polyglyceryl-2 Dihydroxystearate, Glyceryl Sterate Citrate, Sodium Cetearyl Sulfate, Cetearyl Glucoside; Polyglyceryl-6 Stearate (and) Polyglyceryl-6 Behenate, Cetearyl Olive Oil Fatty Acid (and) Sorbitan Olive Oil Fatty Acid, Arachidyl Alcohol (and) Behenyl Alcohol (and) Arachidyl Glucoside, Cetearyl Alcohol (and) Coconut Oil Alkyl Glucoside, Coconut Oil Alkyl Glucoside (and) Coconut Alcohol, PEG-100 Stearate (and) Glyceryl Stearate, Sodium Stearoyl Glutamate, Steareth-20, Steareth-21, Steareth-25, Steareth-2, Ceteth-25, and Ceteth-6 (all described by their INCI names). 【0067】 The most preferred O / W emulsifiers according to the invention are one or more of Ceteth-6 (optionally in a mixture with stearyl alcohol) and Ceteth-25. 【0068】 This at least one O / W or Si / W emulsifier is preferably used in an amount in the range of 0.5 to 10% by weight, particularly in the range of 0.5 to 5% by weight, most specifically in the range of 0.5 to 4% by weight, based on the total weight of the composition. 【0069】 Suitable W / O or W / Si emulsifiers according to the present invention are polyglyceryl-2 dipolyhydroxystearate, PEG-30 dipolyhydroxystearate, cetyl dimethicone copolyol, polyglyceryl-3 diisostearate, polyglycerol esters of oleic acid / isostearic acid, polyglyceryl-6 ricinoleate, polyglyceryl-4 oleate, polyglyceryl-4 oleate / coconut fatty acid PEG-8-PG, magnesium stearate, sodium stearate, potassium laurate, potassium ricinoleate, sodium coconut fatty acid, sodium tallow fatty acid, potassium castor oil fatty acid, sodium oleate, and mixtures thereof. Further suitable W / Si emulsifiers are lauryl polyglyceryl-3 polydimethylsiloxyethyl dimethicone and / or PEG-9 polydimethylsiloxyethyl dimethicone and / or cetyl PEG / PPG-10 / 1 dimethicone and / or PEG-12 dimethicone cross polymer and / or PEG / PPG-18 / 18 dimethicone. A particularly suitable W / O emulsifier for use in the compositions according to the present invention is PEG-30 dipolyhydroxystearate. At least one W / O emulsifier is preferably used in an amount of about 0.001 to 10% by weight, more preferably 0.2 to 7% by weight, based on the total weight of the composition. 【0070】 The sunscreen composition according to the present invention more preferably contains at least one surfactant aid, such as those selected from the group consisting of monoglycerides and diglycerides and / or fatty alcohols. The surfactant aid is generally used in an amount selected from the range of 0.1 to 10% by weight, particularly in the range of 0.5 to 7% by weight, most particularly in the range of 1 to 5% by weight, based on the total weight of the composition. Particularly suitable surfactant aids are selected from the list of alkyl alcohols such as cetyl alcohol (Lorol C16, Lanette 16), cetearyl alcohol (Lanette O), stearyl alcohol (Lanette 18), behenyl alcohol (Lanette 22), glyceryl stearate, glyceryl myristate (Estol 3650), hydrogenated coco-glyceryl (Lipocire Na10), and mixtures thereof. 【0071】 In a particularly advantageous embodiment according to the invention, the emulsifier is selected from the group of ceteth-6 and / or ceteth-25, and the surfactant auxiliary is selected from the group of behenyl alcohol, cetyl alcohol, cetearyl alcohol, and / or stearyl alcohol. 【0072】 The composition in the form of an O / W emulsion according to the invention can be provided, for example, in any formulation form of an O / W emulsion, such as in the form of a lotion, milk, or cream, which are prepared according to conventional methods. The composition which is the subject of the present invention is intended for topical application and can, in particular, constitute a dermatological or cosmetic composition which is intended, for example, to protect human skin from the adverse effects of UV radiation (such as anti-wrinkle, anti-aging, moisturizing, sun protection, etc.). 【0073】 According to an advantageous embodiment of the invention, the composition constitutes a cosmetic composition and is intended for topical application to the skin. 【0074】 According to an advantageous aspect of the invention, the sunscreen composition according to the invention does not contain (i.e., is free of) 3-(4-methylbenzylidene) camphor and 2-hydroxy-4-methoxybenzophenone (INCI: oxybenzone). 【0075】 In another advantageous aspect of the invention, the sunscreen composition according to the invention further contains one or more of dibutyl adipate, dicaprylyl carbonate, stearyl alcohol, alkyl benzoate (C12-15), caprylyl carbonate, tri(caprylic / capric acid) glyceryl, and mixtures thereof, preferably dibutyl adipate, stearyl adipate, and / or dicaprylyl carbonate. 【0076】 In another advantageous aspect of the invention, the sunscreen composition according to the invention further contains ethanol, more preferably in an amount of 0.5% to 10% by weight, more preferably 3% to 8% by weight, based on the total weight of the sunscreen composition. 【0077】 In a further advantageous embodiment of the present invention, the sunscreen composition of the present invention preferably further comprises a preservative and / or a preservative aid selected from the group consisting of phenoxyethanol, ethylhexylglycerin, hexylglycerin, glyceryl caprylate, caprylyl glycol, 1,2 - hexanediol, propanediol, propylene glycol, p - hydroxyacetophenone, and mixtures thereof, and most preferably selected from the group consisting of phenoxyethanol, ethylhexylglycerin, and mixtures thereof. When a preservative or a preservative aid is present, these are preferably used in an amount of 0.01 to 2% by weight, more preferably in an amount of 0.05% to 1.5% by weight, most preferably in an amount of 0.1% to 1.0% by weight, for example in an amount of 0.1% to 0.5% by weight, based on the total weight of the composition. 【0078】 In another advantageous embodiment, the sunscreen composition according to the present invention does not contain any parabens, benzethonium chloride, piroctone olamine, lauroyl arginate, methylisothiazolinone, chloromethylisothiazolinone, bronopol, benzalkonium chloride, formaldehyde - releasing compounds, salicylic acid, triclosan, DMDM hydantoin, chlorphenesin, and IPBC (propargyl iodide butylcarbamate). 【0079】 In a further advantageous embodiment, the sunscreen composition of the present invention further comprises one or more of glycerin or polyhydroxystearic acid, and mixtures thereof. 【0080】 In a further advantageous embodiment, the topical composition of the present invention further comprises a thickener, preferably a gum such as xanthan gum or Caesalpinia spinosa gum or a polyacrylate such as polyacrylate crosspolymer - 6, and mixtures thereof. 【0081】 The sunscreen composition according to the present invention generally has a pH in the range of 3 to 10, preferably in the range of 4 to 8, and most preferably in the range of 4 to 7. This pH can be easily adjusted as needed using appropriate acids such as citric acid or bases such as NaOH according to standard methods in the art. 【0082】 The topical composition according to the present invention can further contain one or more emollient agents that soothe and soften the skin. As an example, the emollient agent can be dicaprylyl carbonate or dibutyl adipate. Further emollient agents are silicone (dimethicone, cyclomethicone), vegetable oil (grape seed, sesame seed, jojoba, etc.), butter (cocoa butter, shea butter), alcohol (stearyl alcohol, cetyl alcohol), and petrolatum derivatives (petrolatum, mineral oil). 【0083】 In still another aspect, the sunscreen composition of the present invention can contain potassium cetyl phosphate as an emulsifier and / or one or more of dibutyl adipate, dicaprylyl carbonate, and stearoyl alcohol. 【0084】 In still another aspect, the sunscreen composition of the present invention can contain acrylic esters such as (acrylates / acrylic acid alkyl (C10 - 30)) crosspolymer, and more preferably, it can be contained in an amount of 0.05 wt% to 1 wt%, more preferably 0.1 wt% to 0.5 wt% based on the total weight of the sunscreen composition. 【0085】 In other embodiments, the sunscreen composition of the present invention can include one or more fragrance substances selected from limonene, citral, linalool, α-isomethylionone, geraniol, citronellol, 2-isobutyl-4-hydroxy-4-methyltetrahydropyran, 2-tert-pentylcyclohexyl acetate, 3-methyl-5-phenyl-1-pentanol, 7-acetyl-1,1,3,4,4,6-hexamethyltetralin, adipic acid diester, cinnamal, amyl salicylate, α-amylcinnamaldehyde, α-methylionone, butylphenylmethylpropanal, cinnamal, amylcinnamyl alcohol, anis alcohol, benzoin, benzyl alcohol, benzyl benzoate, benzyl cinnamate, benzyl salicylate, bergamot oil, bitter orange oil, butylphenylmethylpropional, Zanthoxylum seeds oil, cedrol, cinnamal, cinnamyl alcohol, citronellyl methylcrotonate, citrus oil, coumarin, diethyl succinate, ethyl linalool, eugenol, Evernia furfuracea extract, Pogonatum inflexum extract, farnesol, guaiacwood oil, hexyl cinnamal, hexyl salicylate, hydroxycitronellal, lavender oil, lemon oil, linalyl acetate, mandarin orange oil, PCA menthyl, methyl heptenone, Prunus mume nucifera oil, rosemary oil, sweet orange oil, terpineol, tonka bean oil, triethyl citrate, vanillin. 【0086】 In other embodiments, the sunscreen composition of the present invention can include at least one salt of 2-phenylbenzimidazole-5-sulfonic acid. 【0087】 In other embodiments, the composition can have an SPF of at least 20, preferably at least 30, for example, an SPF of 20 - 50 or 30 - 50. 【0088】 The sunscreen composition of the present invention can achieve the objective even when the total amount of the UV filter is surprisingly small. 【0089】 According to the present invention, advantageously, the sunscreen composition is present in the form of an emulsion or a dispersion, preferably in the form of an emulsion, more preferably in the form of an O / W emulsion. 【0090】 When the sunscreen composition of the present invention is in the form of an O / W emulsion, the formulation advantageously contains one or more O / W emulsifiers selected from glyceryl citrate stearate, glyceryl stearate (self-emulsifying type), stearic acid, stearate, polyglyceryl-3-methylglycose distearate, sodium cetearyl sulfate, potassium cetyl phosphate, polyglyceryl-10 stearate, and sodium stearyl glutamate. 【0091】 According to the present invention, advantageously, these O / W emulsifiers of the present invention can be present in the formulation at a concentration of 0.001 to 10% by weight, preferably 0.1 to 7% by weight, based on the total weight of the formulation. 【0092】 According to the present invention, preferably, the formulation contains potassium cetyl phosphate as an emulsifier. 【0093】 According to the present invention, more advantageously, the formulation contains cetyl alcohol, stearyl alcohol, and / or glyceryl stearate. 【0094】 According to the present invention, advantageously, the formulation of the present invention does not contain polyethylene glycol, polyethylene glycol ether, and polyethylene glycol ester (so-called PEG derivatives). 【0095】 The formulation of the present invention can advantageously contain a humectant. A humectant is a compound or a mixture of compounds that imparts to the cosmetic formulation the quality of reducing the water loss of the stratum corneum (also referred to as transepidermal water loss (TEWL)) and / or having a favorable effect on the hydration of the stratum corneum after the cosmetic formulation is applied or spread on the skin surface. 【0096】 Non-limiting examples of humectants that are advantageous for use in the present invention include glycerol, lactic acid and / or lactate, especially sodium lactate, butylene glycol, propylene glycol, biosaccharide gum-1, azuki bean, ethylhexyl oxyglycerol, pyrrolidone carboxylic acid, and urea. In particular, it is more advantageous to use a high molecular weight humectant from the group of polysaccharides that are water-soluble and / or water-swellable and / or gellable by the power of water. Particularly advantageous are, for example, hyaluronic acid, chitosan, and / or fucose-rich polysaccharides, and the fucose-rich polysaccharide is registered with the registration number 178463-23-5 in the Chemical Abstracts and is available, for example, from SOLABIA S.A. under the name Fucogel® 1000. The humectant can also be advantageously used, for example, as an anti-wrinkle active ingredient for protecting the skin from the types of changes caused by skin aging. 【0097】 The cosmetic preparation of the present invention may, although not essential, advantageously further contain, for example, a filler that further improves the functional and aesthetic properties of the preparation, for example, having an effect of evoking or enhancing the feel of velvet or silk. Advantageous fillers in the context of the present invention are starch and starch derivatives (such as tapioca starch, phosphate-crosslinked starch, aluminum or sodium octenyl succinate starch, etc.), pigments that essentially show neither a UV filtering effect nor a coloring effect (such as boron nitride, etc.), and / or Aerosil® (CAS No. 7631-86-9) and / or talc and / or polyethylene, nylon, and dimethylsilylated silica. 【0098】 As an advantageous embodiment of the formulation of the present invention, an embodiment is also included in which the formulation contains one or more oils selected from dibutyl adipate, dicaprylyl carbonate, and / or alkyl (C12-15) benzoate, dibutyl sebacate, diisopropyl sebacate, dicaprylyl carbonate, dialkyl (C12,13) tartrate, butyloctyl salicylate, diethylhexyl malonate silicylidene, hydrogenated castor oil dimer acid ester, triheptanoin, alkyl (C12,13) lactate, alkyl (C16,17) benzoate, propylheptyl caprylate, glyceryl tri(caprylyl / caprate), diethylhexyl naphthalenedicarboxylate, octyldodecanol, and ethylhexyl coconut oil fatty acid. 【0099】 According to the present invention, a formulation containing dibutyl adipate, dicaprylyl carbonate, and / or alkyl (C12-C15) benzoate is preferred. 【0100】 The aqueous phase of the formulation according to the invention preferably contains conventional cosmetic adjuvants, for example, in each case individually or in combination, alcohols, especially those with a low carbon number, preferably ethanol and / or isopropanol, or polyols with a low carbon number and their ethers, preferably propylene glycol, glycerol, electrolytes, self-tanning agents, and in addition thereto, especially one or more thickeners, preferably thickeners that can be selected from the group of silicon dioxide, aluminum silicate, polysaccharides and / or their derivatives, such as hyaluronic acid, xanthan gum, hydroxypropylmethylcellulose, and particularly preferably thickeners from the group of polyacrylates, preferably from the group called Carbopol (for example, Carbopol types 980, 981, 1382, 2984, and 5984). Advantageous further thickeners according to the invention include those with the INCI name (acrylates / alkyl acrylate (C10 - 30)) cross-polymer (for example, Pemulen TR 1, Pemulen TR 2, Carbopol 1328 from NOVEON), in addition to Aristoflex AVC (INCI: (acryloyldimethyltaurine ammonium / VP) copolymer) and Simugel NS (INCI: (hydroxyethyl acrylate / acryloyldimethyltaurine Na) copolymer (and) squalane (and) polysorbate 60). 【0101】 In this regard, according to the invention, it is preferred that the formulation contains xanthan gum, acrylate / alkyl acrylate (C10 - C30) cross-polymer, (hydroxyethyl acrylate / acryloyldimethyltaurine Na) copolymer (and) squalane (and) polysorbate 60, and / or vinylpyrrolidone / hexadecene copolymer. 【0102】 According to the invention, it is particularly advantageous if the formulation contains at least 2.5% by weight of glycerol based on the total weight of the formulation. 【0103】 According to the present invention, it is advantageous for the formulation to contain one or more alkanediols from the group of 1,2-pentanediol, 1,2-hexanediol, 1,2-octanediol, 1,2-decanediol, 2-methyl-1,3-propanediol. 【0104】 According to the present invention, it is advantageous for the formulation of the present invention to contain ethanol, phenoxyethanol, and / or ethylhexylglycerin. 【0105】 According to the present invention, the formulation of the present invention preferably contains a film-forming agent. The film-forming agent in the sense of the present invention is various kinds of substances characterized by having the following properties: when the film-forming agent is dissolved in water or other appropriate solvents and this solution is applied to the skin, after the solvent evaporates, the film-forming agent forms a film, and this film basically serves to fix the UV filter on the skin, thereby enhancing the SPF and / or water resistance of the product. 【0106】 It is particularly advantageous to select the film-forming agent from the group of polymers based on polyvinylpyrrolidone (PVP). 【0107】 For example, copolymers of vinylpyrrolidone such as (VP / hexadecene) copolymer and (VP / eicosene) copolymer available under the trade names Antaron V216 and Antaron V220 from GAF Chemicals Corporation are particularly preferred. 【0108】 Similarly, other polymeric film-forming agents are also advantageous, such as sodium polystyrene sulfonate available under the trade name Flexan 130 from National Starch and Chemical Corp. and / or polyisobutene available under the trade name Rewopal PIB1000 from Rewo. Examples of further suitable polymers are polyacrylamide (Seppigel 305), polyvinyl alcohol, PVP, PVP / VA copolymer, polyglycol, acrylate / octylacrylamide copolymer (Dermacryl 79). It is similarly advantageous to use hydrogenated castor oil dimer dilinoleate (CAS 646054-62-8, INCI: Hydrogenated Castor Oil Dimer Dilinoleate) available under the name Risocast DA-H from Kokyu Alcohol Kogyo or PPG-3 benzyl ether myristate (CAS 403517-45-3) available under the trade name Crodamol STS from Croda Chemicals. 【0109】 The following examples are provided to further illustrate the compositions and effects of the present invention. These examples are merely illustrative and are not intended to limit the scope of the present invention in any way. 【0110】 [Examples] [in vitro UV model] The microbial strains used in this test are listed below. The restoration of the cultures is carried out according to the instructions of CRBIP (Institut Pasteur Collection, France) or ATCC (American Type Culture Collection, USA), which are the collection centers of the reference strain suppliers: - Lactobacillus crispatus CIP 103604 - Staphylococcus epidermidis ATCC 12228 【0111】 A 96-well plate was filled with medium (140 μL), and the adjusted inoculum of each strain (20 μL) was added such that the final concentration was 1.106 colony-forming units per milliliter (cfu / mL). After addition, the sample (40 μL; 20% v / v) was added in triplicate and mixed well to ensure uniform distribution. Then, a UV load was applied to the plate using a UV irradiation device (BioSun, Vilber Lourmat, France) according to the UV irradiation doses shown in Table 1 below. This specific UV irradiation dose was selected such that the survival rate of the microbial population was between 10 and 49%. 【0112】 【Table 1】 【0113】 To count the number of microbial individuals, samples with Lactobacillus crispatus (L. crispatus) and Staphylococcus epidermidis (S. epidermidis) added were aliquoted and serially diluted (4 times with 10-fold dilution followed by 8 times with 2-fold dilution, in triplicate) in medium on a 96-well plate containing triphenyltetrazolium. Then, considering the respiratory type of each strain, the plates were incubated at 37 °C ± 2.5 °C for 48 hours. After 48 hours under optimal growth conditions, the contamination rate was indicated from the last dilution showing pink color (triphenyltetrazolium) or turbidity, and it was possible to determine the reduction rate of the number of microbial individuals (most probable number (MPN) method). In this experiment, a medium containing bacteria cultured without UV load was used as a control. The number of microbial individuals was expressed as cfu / mL and was taken as the average value of the experiments performed in triplicate. 【0114】 The use concentrations of single UV filters and their combinations in the test formulation were calculated such that the predicted in vitro SPF values were 5.75 + / - 0.15 and 23.45 + / - 0.65, respectively (Table 2). The UV filters were mixed in two oils, namely Tegosoft XC (55%) and Miglyol 829 Eco (the remaining amount up to 100%), and the emulsifier Tween 80 (5%). 【0115】 In the presence of test samples 1 to 10 at 20% v / v, the effect of the selected UV irradiation conditions on the survival of the microbial populations of Lactobacillus crispatus (L. crispatus) and Staphylococcus epidermidis (S. epidermidis) was compared with the corresponding strains (controls) not exposed to UV irradiation and without the presence of samples 1 to 10. The results after 4 hours of culture were compared with the number of microbial individuals cultured without UV load (determination of the number of surviving microbial individuals) and expressed as the survival rate. The relative protection rate (%) was calculated by dividing the difference in the survival rate between the sample and the solvent control (without UV irradiation) by the survival rate of the solvent control. The survival rate of each microbial individual number and the relative protection rate by the UV filter thus calculated are summarized in Table 2. 【0116】 [Table 2] 【0117】 [Table 3] 【0118】 [In vivo test composition] In this single-blind placebo-controlled clinical trial, the effect of UV irradiation on the skin microbiome composition of human skin and the protection of the skin microbiome by a representative SFP20 sunscreen compared to a placebo formulation were specifically evaluated. 【0119】 To evaluate the ability to protect the UV-exposed skin microbiome, a test product composed of two test formulations was used, one of which included broad-spectrum UV protection by four UV filters (i.e., PARSOL® Shield, PARSOL® 340, PARSOL® EHS, and PARSOL® 1789), and the other was a solvent formulation without UV filters ("placebo"): 1. Placebo formulation containing only the solvent; the UV filter portion was replaced by increasing the oil content. 2. SPF20 Sunscreen Formulation 【0120】 【Table 4】 【0121】 After explaining to and obtaining consent from the subjects, the test formulation was applied to the backs of 10 healthy female subjects (aged 22 - 43 years, Fitzpatrick skin types II - III), and then irradiated with UV at 2 minimal erythema doses (2MED). Two test areas of approximately 25 cm² of skin were each applied with approximately 2 mg per 1 cm² of body surface area with two test formulations. The individual MED of each subject was measured the day before this treatment. To perform the analysis before UV irradiation, samples were collected by swabbing the skin surface of the untreated and unirradiated area. Three defined areas on the back (approx. 25 cm²) were exposed to 2MED of UV radiation using a single-port solar simulator. To perform the analysis, 2 hours after UV exposure, the skin of the three irradiated areas was swabbed to collect samples. 2 of two test areas were each applied with approximately 2 mg per 1 cm² of body surface area with two test formulations. 2 of body surface area with two test formulations. The individual MED of each subject was measured the day before this treatment. To perform the analysis before UV irradiation, samples were collected by swabbing the skin surface of the untreated and unirradiated area. Three defined areas on the back (approx. 25 cm²) were exposed to 2MED of UV radiation using a single-port solar simulator. To perform the analysis, 2 hours after UV exposure, the skin of the three irradiated areas was swabbed to collect samples. 2 ) were exposed to 2MED of UV radiation using a single-port solar simulator. To perform the analysis, 2 hours after UV exposure, the skin of the three irradiated areas was swabbed to collect samples. 【0122】 DNA was extracted from the swab samples of 9 completers using the ZymoBIOMICS DNA Miniprep Kit according to the manufacturer's protocol, and sequenced on an Illumina MiSeq in a 2×300 paired-end configuration. Primers targeting the V3 - V4 region of the bacterial 16S rRNA gene were used. The sequences were then processed into amplicon sequence variants (ASVs). 【0123】 Using the software tool called Songbird, which is outlined in Morton et al. (Nat. Commun., 2019. 10(1): p. 2719), the ranking of taxonomic differences was investigated from the perspective of 16S rDNA for the categorical variables of irradiation, treatment, and verum. Songbird gives the relationship between each feature and the categorical variable or quantitative measurement by modeling the ranking based on this difference. As a result, these different features include coefficients that describe the relationship with the modeled variable. 【0124】 [In vivo results] Ten white female subjects aged 22 - 43 years (mean age 32 ± 2 years, Fitzpatrick skin type II (4 subjects) and III (6 subjects)) were enrolled in and completed the study. No adverse effects were observed during the study period. 【0125】 Based on 16S rRNA sequence analysis of samples obtained from 9 completers, Lactobacillus crispatus was confirmed to show a positive association only with the formulation containing the UV filter. On the other hand, a negative association was shown with irradiation and the placebo formulation. 【0126】 【Table 5】 【0127】 As observed in Lactobacillus crispatus, adding UV protection will benefit certain microorganisms. Interestingly, it is extremely rare for Lactobacillus crispatus (L. crispatus) to be described as a skin commensal. This dominant vaginal bacterium plays an important role by forming beneficial biofilms that protect the host's tissues and functioning as components of the innate immune response. These create an acidic environment (pH about 4 - 4.5) by metabolizing glucose into lactic acid, perform competitive exclusion, and release antibacterial compounds such as H2O2, bacteriocins, and other by-products, resulting in the maintenance of skin self-defense and skin microbiota symbiosis.

Claims

[Claim 1] A composition comprising one or more UV filters selected from the group consisting of octocrylene, t-butyl methoxydibenzoylmethane, and ethylhexyl triazone, for use in the treatment or prevention of skin dysbiosis after exposure to UV radiation, and / or for strengthening the skin surface barrier. [Claim 2] A composition according to claim 1 for use in the treatment or prevention of dysbiosis of the skin after UV radiation exposure, and / or for strengthening the skin surface barrier, wherein the Lactobacillus bacteria and Staphylococcus epidermidis are present on the outer surface of a human body having a skin microbiome containing the bacteria. [Claim 3] The composition according to claim 2, characterized in that the protection of bacteria of the genus Lactobacillus results in a survival rate of at least 100% after exposure to UV radiation compared to the control. [Claim 4] The composition according to claim 2, characterized in that the protection of Staphylococcus epidermidis increases the survival rate after exposure to UV radiation by at least 20% compared to the control. [Claim 5] The composition according to claim 1 or 2, wherein the amount of the one or more UV filters in the composition is selected such that the composition exhibits an SPF of at least 20. [Claim 6] The composition according to claim 2, wherein the skin bacterium of the genus Lactobacillus is Lactobacillus crispatus. [Claim 7] The composition according to claim 2, wherein the Lactobacillus genus skin bacterium is Lactobacillus crispatus CIP 103604. [Claim 8] The composition according to claim 2, wherein the Staphylococcus epidermidis is Staphylococcus epidermidis ATCC 12228. [Claim 9] The composition according to claim 1 or 2, wherein the amount of ethylhexyl triazone in the composition is selected within the range of 0.1% to 10% by weight, based on the total weight of the composition. [Claim 10] The composition according to claim 1 or 2, wherein the amount of t-butylmethoxydibenzoylmethane in the composition is selected within the range of 0.1% to 10% by weight, based on the total weight of the sunscreen composition. [Claim 11] The composition according to claim 1 or 2, wherein the amount of octocrylene in the composition is selected within the range of 0.1% by weight to 10% by weight, based on the total weight of the composition. [Claim 12] A method for protecting bacteria of the genus Lactobacillus and Staphylococcus epidermidis from the harmful effects of UV radiation, comprising the step of applying an effective amount of a sunscreen composition containing one or more UV filters selected from the group consisting of octocrylene, t-butyl methoxydibenzoylmethane, ethylhexyl triazone, and zinc oxide to the outer surface of a human body having a skin microbiome containing the bacteria, before exposing the outer surface to UV radiation. [Claim 13] A method for increasing the difference in the abundance of bacteria of the genus Lactobacillus in the skin microbiome of an individual after exposure to UV radiation, comprising the step of topically administering an effective amount of a composition comprising one or more UV filters selected from the group consisting of octocrylene, t-butylmethoxydibenzoylmethane, and ethylhexyl triazone to an external surface of the body in need, before the external surface is exposed to UV radiation. [Claim 14] The method according to claim 13, wherein the composition comprises octocrylene, ethylhexyl salicylate, bis-ethylhexyloxyphenol methoxyphenyl triazine, and t-butyl methoxydibenzoylmethane. [Claim 15] The method according to claim 14, wherein the total amount of UV filter is selected within the range of 15 to 20% by weight based on the total weight of the composition.