Use of a UV filter for the treatment of acne

Abstract

The present invention relates to the use of a selected UV filter selected from the group consisting of octocrylene, ethylhexyl salicylate and butyl methoxydibenzoylmethane for reducing the abundance of C. acnes strains in the skin microbiota after UV radiation. Said use is suitable for preventing and / or treating the progression of acne, in particular the progression of acne exacerbated by the application of sunscreen.

Description

Detailed Description of the Invention 【0001】 The present invention relates to the use of a selected UV filter selected from the group consisting of octocrylene, ethylhexyl salicylate and butyl methoxydibenzoylmethane for reducing the abundance of C. acnes strains in the skin microbiota after UV radiation. Said use is suitable for preventing and / or treating the progression of acne, in particular the progression of acne exacerbated by the application of sunscreen. 【0002】 The skin is the outermost protective cover of an organism and the largest organ in the body. It functions as a barrier to protect the body from external factors. Furthermore, it is well known that a wide variety of microorganisms that form the skin microbiota (often also called the skin microbiome) colonize the surface of the skin. Most of these microorganisms are symbiotic organisms that live in a mutualistic relationship with the immune system of the skin. Dysbiosis in the skin microbiota is associated with many skin pathologies such as acne, atopic dermatitis and psoriasis. 【0003】 Acne (a common term for acne vulgaris) is a common chronic skin problem that affects the pilosebaceous unit. It is estimated that approximately 85% of adolescents and young adults suffer from this disease. Acne generally appears as inflamed papules, pustules or nodules. Inflammation can further be associated with redness, swelling and tenderness. Acne has been established to correlate with the presence of specific opportunistic pathogenic Cutibacterium acnes (C. acnes) strains. The strains of the C. acnes population are divided into six major phylotypes: IA1, IA2, IB, IC, II and III. In patients with severe acne, phylotype IA1 has been found to be very dominant. 【0004】 It is well established that sunscreens can promote or exacerbate acne. Additionally, acne has been suggested to be caused by an imbalance in the skin microbiota associated with certain types of pathogenic Cutibacterium acnes strains. Therefore, modulation of the population of such C. acnes strains on the skin by the use of a particular sunscreen may be useful by applying such a sunscreen to acne-prone skin and preventing the formation or exacerbation of acne after such use. 【0005】 Therefore, there remains a need to reduce pathogenic C. acnes strains in the skin microbiota following sunscreen application and UV exposure. 【0006】 Accordingly, the inventors developed a targeted in vitro liquid model in a 96-well microplate format for screening UV-protective active substances (“UV filters”) against bacterial survival. The effect of selected UV irradiation conditions on the microbial populations of C. acnes and the commensal reference strain Staphylococcus epidermidis was tested in the presence of various UV filters and their combinations, and was evaluated by the development of the residual microbial population after 4 hours. The inhibition of bacterial growth as a result of UV irradiation was expressed as a percentage of survival, compared to the inhibition of the same strain without UV irradiation. 【0007】 Surprisingly, it has now been found that certain UV filters can be suitable for reducing the overall amount of pathogenic C. acnes after UV radiation. 【0008】 Accordingly, in a first embodiment, the present invention relates to a method for reducing C. acnes, the method comprising applying to the outer surface of a human body having a skin microbiota comprising C. acnes and / or having acne-prone skin an effective amount of a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene (OC), ethylhexyl salicylate (EHS), and butyl methoxydibenzoylmethane (BMDBM) prior to exposing the outer surface to UV radiation. 【0009】 In another embodiment, the present invention relates to the use of one or more UV filters selected from the group consisting of octocrylene, ethylhexyl salicylate, and butyl methoxydibenzoylmethane for reducing the abundance of C. acnes in the skin microbiota during UV irradiation, in particular for preventing and / or treating the progression of acne, most particularly the progression of acne exacerbated by the application of sunscreen. 【0010】 In all embodiments of the present invention, preferably concomitantly, the viability of the skin commensal Staphylococcus epidermidis is also increased by the application of one or more UV filters selected from the group consisting of octocrylene, ethylhexyl salicylate, and butyl methoxydibenzoylmethane. Accordingly, in preferred embodiments, all methods and uses disclosed herein include the concomitant protection of Staphylococcus epidermidis from the harmful effects of UV radiation as compared to untreated controls. 【0011】 All methods and uses disclosed herein are particularly suitable for treating or preventing dysbiosis and, as a result, for enhancing the surface barrier and immune function of the skin after UV radiation. The methods and uses disclosed herein are also suitable for promoting a healthy skin microbiota after UV radiation by being able to reduce the abundance of pathogenic C. acnes strains while increasing the abundance of Staphylococcus epidermidis as compared to untreated controls. 【0012】 For the reduction of numbers, i.e., the reduction in the abundance of C. acnes after UV radiation, the metabolites secreted by said bacteria may also be reduced. 【0013】 Without wishing to be bound by theory, these metabolites are thought to contribute to the progression of acneic skin. Thus, in another aspect, the present invention relates to the use of a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, ethylhexyl salicylate, and butyl methoxydibenzoylmethane for preventing or treating acne by maintaining the balance of the skin microbiota after exposure to UV radiation. 【0014】 In a further aspect, the present invention also relates to a method for preventing or treating acne by maintaining the balance of the microbiota (i.e., by reducing C. acnes and increasing S. epidermidis compared to untreated controls), said method comprising, prior to UV radiation, applying to the surface of the human body a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, ethylhexyl salicylate, and butyl methoxydibenzoylmethane, subsequently exposing said surface to UV radiation, and optionally determining the effect. Preferably, the surface of the human body is at least one of the skin or the scalp. The method according to this aspect can be therapeutic or non-therapeutic. In an advantageous embodiment, the method is non-therapeutic and includes cosmetics, i.e., those intended to beautify the skin. In another advantageous embodiment, the method is therapeutic, for example, for treating acne. 【0015】 Reduction of Cutibacterium acnes, for example Cutibacterium acnes ATCC 11827, which is known to be an opportunistic pathogen belonging to phylotype IA, according to the present invention, is characterized in that the viability of Cutibacterium acnes is, for example, in particular at least -10%, preferably at least -30%, most preferably at least -60%, for example -10, -30 or -60 to -100% or -10, -30 or -60 to -75% less than the control after exposure to UV radiation at 3 standard erythema doses SED (1 SED = 100 J / m2). 【0016】 Protection of Staphylococcus epidermidis according to the present invention is characterized in that the viability of Staphylococcus epidermidis increases by at least 20%, preferably at least 50%, for example +20 to +100% or +50 to +100% compared to the control after exposure to UV radiation (at 15 SED). 【0017】 Particularly preferred UV filters according to the present invention are ethylhexyl salicylate and butyl methoxydibenzoylmethane, most preferably butyl methoxydibenzoylmethane, which provide a particularly good reduction in the viability of Cutibacterium acnes such as Cutibacterium acnes ATCC 11827. 【0018】 The total amount of UV filter in the sunscreen composition strongly depends on the object of protection and the UV radiation to which it is exposed. Preferably, in all embodiments of the present invention, the amount of UV filter is selected such that the sunscreen composition exhibits an SPF of at least 20, preferably at least 25, most preferably at least 30, for example in the range of 20 to 100, 20 to 50, 25 to 100, 25 to 50, 30 to 100 or 30 to 50. 【0019】 A sunscreen composition with an SPF of 20 contains, for example, a total amount of UV filters preferably in the range of 10 to 40% by weight, more preferably in the range of 15 to 25% by weight, based on the total weight of the composition. 【0020】 A sunscreen composition with an SPF of 50 contains, for example, a total amount of UV filters preferably in the range of 15 to 50% by weight, more preferably in the range of 20 to 40% by weight, based on the total weight of the composition. 【0021】 In all embodiments of the present invention, a particularly preferred bacterium of the genus C. acnes is Cutibacterium acnes ATCC 11827. 【0022】 As used herein, the term UV radiation (used synonymously with UV light) refers to radiation in the range of UVB to UVA of 280 to 400 nm. 【0023】 As used herein, the term "outer surface of the human body" refers to the skin and the scalp. Preferably, in all embodiments of the present invention, the outer surface of the human body treated according to the present invention is the skin of the face, neck and / or body. 【0024】 As used herein, the term "skin microbiota" refers to a group of microorganisms that colonize a defined skin area of an individual, such as, but not limited to, the forehead, forearm, cheek or scalp. 【0025】 In all embodiments of the present invention, the amount of 2-ethylhexyl salicylate (also known as octyl salicylate) in the sunscreen composition according to the present invention is preferably selected from the range of 0.1 to 20% by weight, more preferably from the range of 0.1 to 10% by weight, and most preferably from the range of 0.1 to 5% by weight, based on the total weight of the composition. 【0026】 In all embodiments of the present invention, the amount of butyl methoxydibenzoylmethane (also known as avobenzone, 3-(4-tert-butylphenyl)-1-(4-methoxyphenyl)propane-1,3-dione respectively) in the sunscreen composition according to the present invention is preferably in the range of 0.1% by weight to 10% by weight, more preferably in the range of 0.5% by weight to 7.5% by weight, and most preferably in the range of 1% by weight to 5% by weight, based on the total weight of the sunscreen composition. 【0027】 In all embodiments of the present invention, the amount of octocrylene (also known as 2-ethylhexyl 2-cyano-3,3-diphenylprop-2-enoate, ethylhexyl 2-cyano-3,3-diphenylacrylate respectively) in the sunscreen composition according to the present invention is preferably in the range of 0.1% by weight to 10% by weight, more preferably in the range of 0.5% by weight to 7.5% by weight, and most preferably in the range of 1% by weight to 5% by weight, based on the total weight of the sunscreen composition. 【0028】 In certain embodiments, the sunscreen composition according to the present invention contains octocrylene, ethylhexyl salicylate and butyl methoxydibenzoylmethane in an amount of more preferably 15 to 20% by weight, based on the total weight of the sunscreen composition. 【0029】 In another specific embodiment, the sunscreen composition preferably comprises ethylhexyl salicylate and ethylhexyl triazone (EHT) or ethylhexyl triazone, bis-ethylhexyloxyphenol methoxyphenyl triazine (BEMT) and butyl methoxydibenzoylmethane, more preferably consisting only of these. The amount of EHT is preferably selected from the range of 0.1% to 10% by weight, more preferably from the range of 0.5% to 7.5% by weight, and most preferably from the range of 1% to 5% by weight based on the total weight of the sunscreen composition. The amount of BEMPT is preferably selected from the range of 0.1% to 10% by weight, more preferably from the range of 0.5% to 7.5% by weight, and most preferably from the range of 0.5% to 5% by weight based on the total weight of the sunscreen composition. 【0030】 Since the sunscreen compositions according to the present invention are intended for topical application, it is well understood that they include a physiologically acceptable medium, i.e., a medium that is compatible with horny substances such as the skin, mucous membranes and keratin fibers. In particular, the physiologically acceptable medium is a cosmetically acceptable carrier. 【0031】 As used herein, the term "cosmetically acceptable carrier" refers to all carriers and / or excipients and / or diluents conventionally used in topical cosmetic compositions such as skin care preparations. 【0032】 The exact amount of the carrier will depend on the actual levels of the UV filter and any other optional components (e.g., other active ingredients) that a person skilled in the art would classify as different from the carrier. 【0033】 In an advantageous embodiment, the sunscreen composition according to the present invention comprises 50% to 99%, preferably 60% to 98%, more preferably 70% to 98%, for example particularly 80% to 95% of the carrier based on the total weight of the sunscreen composition. 【0034】 In certain advantageous embodiments, the carrier further consists of at least 40% by weight, more preferably at least 50% by weight, most preferably at least 55% by weight of water, for example in particular from 55 to 90% by weight of water. 【0035】 In particular, the sunscreen composition according to the invention is a cosmetic or pharmaceutical composition, preferably a cosmetic (non-therapeutic) composition. 【0036】 In one embodiment, the sunscreen composition according to the invention is applied to mammalian keratinous tissues, for example in particular human skin or human scalp and hair. 【0037】 As used in this application, the term "cosmetic composition" refers to the cosmetic compositions defined under the heading "Kosmetika" in Roempp Lexikon Chemie, 10th edition 1997, Georg Thieme Verlag Stuttgart, New York and the cosmetic compositions disclosed in A. Domsch, "Cosmetic Compositions", Verlag fuer chemische Industrie (ed. H. Ziolkowsky), 4th edition, 1992. 【0038】 The sunscreen composition according to the invention is also known as a photoprotective preparation (sun care product, sunscreen), such as a sunscreen emulsion, a sunscreen lotion, a sunscreen cream, a sunscreen oil, a sunscreen agent or a tropical cream or day care cream having an SPF (sun protection factor). Sunscreen creams, sunscreen lotions, sunscreen emulsions and sunscreen preparations are of particular interest. 【0039】 The composition of the present invention (including a carrier) may contain conventional adjuvants and additives such as preservatives / antioxidants, fatty substances / oils, organic solvents, silicones, thickeners, softeners, emulsifiers, defoamers, aesthetic components such as fragrances, surfactants, fillers, anionic, cationic, non-ionic or amphoteric polymers or mixtures thereof, propellants, acidifying or basifying agents, dyes, colorants / coloring agents, abrasives, absorbents, chelating agents and / or sequestering agents, essential oils, skin cooling agents, astringents, pigments or any other components normally incorporated into such compositions. 【0040】 According to the present invention, the composition according to the present invention may contain components for skin whitening, sunburn prevention, treatment of hyperpigmentation, acne, wrinkles, fine lines, atrophy and / or prevention or reduction of inflammation, chelating agents and / or sequestering agents, anti-cellulite and slimming (e.g., phytic acid), tightening, moisturizing and activating, self-tanning, soothing and agents for improving elasticity and skin barrier properties and / or further UV filter substances and carriers and / or excipients or diluents and the like, which have been conventionally used in sunscreen compositions. 【0041】 Unless otherwise stated, the excipients, additives, diluents and the like described below are suitable for the sunscreen composition according to the present invention. The required amounts of cosmetic and dermatological adjuvants and additives can be readily determined by those skilled in the art based on the desired product. 【0042】 The additional components can be added to the oil phase, the aqueous phase or added individually, whichever is considered appropriate. The mode of addition can be readily adapted by those skilled in the art. 【0043】 Examples of cosmetic excipients, diluents, adjuvants, additives and active ingredients commonly used in the skin care industry that are suitable for use in the cosmetic compositions of the present invention are described, for example, but not limited to, in the International Cosmetic Ingredient Dictionary & Handbook by the Personal Care Product Council (http: / / www.personalcarecouncil.org / ) accessible by the ONLINE INFO BASE (http: / / online.personalcarecouncil.org / jsp / Home.jsp). 【0044】 Cosmetic active ingredients useful herein may, in some cases, provide more than one benefit or function by more than one mechanism of action. 【0045】 Of course, those skilled in the art will take care to select the above optional additional ingredients, adjuvants, diluents and additives and / or their amounts so that the advantageous properties inherent in the combination according to the invention are not adversely affected or are substantially not affected by the envisaged addition. 【0046】 The cosmetic compositions according to the invention can be in the form of a suspension or dispersion in a solvent or fatty substance or alternatively in the form of an emulsion or microemulsion (in particular oil-in-water (O / W) or water-in-oil (W / O) type, silicone-in-water (Si / W) or water-in-silicone (W / Si) type, PIT-emulsion, multiple emulsion (e.g. oil-in-water-in-oil (O / W / O) or water-in-oil-in-water (W / O / W) type), pickering emulsion, hydrogel, alcoholic gel, lipogel, single-phase or multiphase solution or vesicular dispersion or in other conventional forms that can be applied as a mask or as a spray by means of a pen. 【0047】 The sunscreen composition according to the present invention is preferably in the form of an oil-in-water (O / W) emulsion comprising an oil phase dispersed in an aqueous phase in the presence of an O / W emulsifier. The preparation of such O / W emulsions is well known to those skilled in the art and is illustrated in the examples. 【0048】 In an advantageous embodiment, the O / W emulsifier is a phosphate ester emulsifier. Among the preferred phosphate ester emulsifiers, C 8~10 alkyl ethyl phosphate, C 9~15 alkyl phosphate, ceteareth-2 phosphate, ceteareth-5 phosphate, cetes-8 phosphate, cetes-10 phosphate, cetyl phosphate, C 6~10 pares-4 phosphate, (C 12~15 ) pares-2 phosphate, (C 12~15 ) pares-3 phosphate, ceteareth-2 phosphate DEA (DEA-Ceteareth-2 Phosphate), cetyl phosphate DEA, oles-3 phosphate DEA, potassium cetyl phosphate, deceth-4 phosphate, deceth-6 phosphate and trilauryl-4 phosphate may be mentioned. A specific phosphate ester emulsifier according to the present invention is, for example, potassium cetyl phosphate commercially available as Amphisol® K from DSM Nutritional Products Ltd Kaiseraugst. 【0049】 More suitable O / W emulsifiers according to the present invention include PEG-30 dipolyhydroxystearate, PEG-4 dilaurate, PEG-8 dioleate, PEG-40 sorbitan peroleate, PEG-7 glyceryl cocoate, PEG-20 almond glyceride, PEG-25 hydrogenated castor oil, glyceryl stearate (and) PEG-100 stearate, PEG-7 oleate, PEG-8 oleate, PEG-8 laurate, PEG-60 almond glyceride, PEG-20 methyl glucose sesquistearate, PEG-40 stearate, PEG-100 stearate, PEG-80 sorbitan laurate, steareth-2, steareth-12, oleth-2, ceteareth-2, laureth-4, oleth-10, oleth10 / polyoxyl 10 oleyl ether, ceteareth-10, isosteareth-20, ceteareth-20, oleth-20, steareth-20, steareth-21, ceteareth-20, isoceteareth-20, laureth-23, steareth-100, glyceryl stearatocitrate, glyceryl stearate (self-emulsifying), stearic acid, salts of stearic acid, polyglyceryl-3-methylglycosedistearate. Even more suitable emulsifiers are sorbitan oleate, sorbitan sesquioleate, sorbitan isostearate, sorbitan trioleate, lauryl glucoside, decyl glucoside, sodium stearoyl glutamate, sucrose polystearate and hydrated polyisobutene. Further, one or more synthetic polymers can be used as emulsifiers. For example, (VP / eicosene) copolymer, (acrylates / alkyl acrylate (C 10~30 )) cross-polymer, (acrylates / steareth-20 methacrylate) copolymer, (PEG-22 / dodecyl glycol) copolymer, (PEG-45 / dodecyl glycol) copolymer and mixtures thereof. 【0050】 Another particularly suitable type of O / W emulsifier is, for example, the non-ionic self-emulsifying system derived from olive oil known as cetearyl olivate and sorbitan olivate (chemical composition: sorbitan esters and cetearyl esters of olive oil fatty acids), sold under the trade name OLIVEM 1000. 【0051】 Commercially available polymer emulsifiers, such as hydrophobically modified polyacrylic acids like (acrylates / alkyl acrylates (C10 - 30)) crosspolymer sold under the trade names Pemulen(®) TR-1 and TR-2 from Noveon, are even more suitable. 【0052】 Another particularly suitable type of emulsifier is the polyglycerol ester or diester of fatty acids, also called polyglyceryl ester / diester (i.e., the polymer in which the fatty acid () is bonded by esterification with polyglycerol), such as Isolan GPS [INCI name (diisostearic acid / polyhydroxystearic acid / sebacic acid) polyglyceryl - 4 (i.e., the diester of a mixture of isostearic acid, polyhydroxystearic acid, and sebacic acid and polyglyceryl - 4)] commercially available from Evonik or Dehymuls PGPH (INCI dipolyhydroxystearic acid polyglyceryl - 2) commercially available from Cognis. 【0053】 Polyethylene glycol ethers, such as Brij 72 (polyoxyethylene (2) stearyl ether) or Brij 721 (polyoxyethylene (21) stearyl ether) available from Croda, are also suitable. 【0054】 Particularly advantageous O / W emulsifiers according to the invention are one or more of polyglyceryl-3 methylglucose distearate, lauryl glucoside (and) polyglyceryl-2 dipolyhydroxystearate, glyceryl citrate stearate, sodium cetearyl sulfate, cetearyl glucoside; polyglyceryl-6 stearate (and) polyglyceryl-6 behenate, cetearyl olive oil fatty acid (and) sorbitan olive oil fatty acid, arachidyl alcohol (and) behenyl alcohol (and) arachidyl glucoside, cetearyl alcohol (and) coconut alkyl glucoside, coconut alkyl glucoside (and) coconut alcohol, PEG-100 stearate (and) glyceryl stearate, sodium stearoyl glutamate, stearess-20, stearess-21, stearess-25, stearess-2, cetearess-25 and cetearess-6 (all listed by their INCI names). 【0055】 The most preferred O / W emulsifiers according to the invention are one or more of cetearess-6 (optionally in a mixture with stearyl alcohol) and cetearess-25. 【0056】 At least one O / W, Si / W emulsifier is each preferably used in an amount in the range of 0.5 to 10% by weight, particularly in the range of 0.5 to 5% by weight, most particularly in the range of 0.5 to 4% by weight, etc., based on the total weight of the composition. 【0057】 Suitable W / O or W / Si emulsifiers according to the present invention are polyglyceryl-2-dipolyhydroxystearate, PEG-30 dipolyhydroxystearate, cetyl dimethicone copolyol, polyglyceryl-3 diisostearate polyglycerol ester of oleic acid / isostearic acid, polyglyceryl-6 hexaricinoleate, polyglyceryl-4 oleate, polyglyceryl-4 / PEG-8 propylene glycol coconut fatty acid oleate, magnesium stearate, sodium stearate, potassium laurate, potassium ricinoleate, sodium coconut fatty acid, sodium tallowate, potassium castorate, sodium oleate, and mixtures thereof. Further suitable W / Si emulsifiers are lauryl polyglyceryl-3 polydimethylsiloxyethyl dimethicone and / or PEG-9 polydimethylsiloxyethyl dimethicone and / or cetyl PEG / PPG-10 / 1 dimethicone and / or PEG-12 dimethicone cross polymer and / or PEG / PPG-18 / 18 dimethicone. A particularly suitable W / O emulsifier for use in the composition according to the present invention is PEG-30 dipolyhydroxystearate. At least one W / O emulsifier is preferably used in an amount of about 0.001 to 10% by weight, more preferably 0.2 to 7% by weight, based on the total weight of the composition. [[ID=]1] 【0058】 The sunscreen composition according to the present invention more preferably contains at least one surfactant aid selected from the group consisting of, for example, monoglycerides and diglycerides and / or fatty alcohols. The surfactant aid is generally used in an amount selected from the range of 0.1 to 10% by weight, particularly 0.5 to 7% by weight, and most particularly 1 to 5% by weight, based on the total weight of the composition. Specific suitable surfactant aids are selected from the list of alkyl alcohols such as cetyl alcohol (Lorol C16, Lanette 16), cetearyl alcohol (Lanette O), stearyl alcohol (Lanette 18), behenyl alcohol (Lanette 22), glyceryl stearate, glyceryl myristate (Estol 3650), hydrogenated coco-glyceryl (Lipocire Na10), and mixtures thereof. 【0059】 In a particularly advantageous embodiment according to the invention, the emulsifier is selected from the group of cetearyl-6 and / or cetearyl-25, and the surfactant adjuvant is selected from the group of behenyl alcohol, cetyl alcohol, cetearyl alcohol and / or stearyl alcohol. 【0060】 The composition in the form of an O / W emulsion according to the invention can be provided, for example, in any formulation form of an O / W emulsion, such as in the form of a lotion, milk or cream, which are prepared according to conventional methods. The composition which is the subject of the present invention is intended for topical application and, in particular, can constitute a dermatological or cosmetic composition, for example, intended to protect human skin from the adverse effects of UV radiation (such as wrinkle prevention, anti-aging, moisturizing, sun protection, etc.). 【0061】 According to an advantageous embodiment of the invention, the composition constitutes a cosmetic composition and is intended for topical application to the skin. 【0062】 In an advantageous aspect of the invention, the sunscreen composition according to the invention does not contain (i.e., does not include) 3-(4-methylbenzylidene)-camphor and 2-hydroxy-4-methoxybenzophenone (INCI: oxybenzone). 【0063】 In another advantageous aspect of the invention, the sunscreen composition according to the invention further comprises one or more of dibutyl adipate, dicaprylyl carbonate, stearyl alcohol, alkyl benzoate (C12-15), caprylyl carbonate, tri(caprylic / capric acid) glyceryl and mixtures thereof, preferably dibutyl adipate, stearyl adipate and / or dicaprylyl carbonate. 【0064】 In another advantageous aspect of the invention, the sunscreen composition according to the invention further comprises ethanol in an amount of more preferably 0.5 to 10% by weight, more preferably 3 to 8% by weight, based on the total weight of the sunscreen composition. 【0065】 In a further advantageous embodiment of the present invention, the sunscreen composition of the present invention preferably further comprises a preservative and / or a preservative aid selected from the group consisting of phenoxyethanol, ethylhexylglycerin, hexylglycerin, glyceryl caprylate, caprylyl glycol, 1,2 - hexanediol, propanediol, propylene glycol, p - hydroxyacetophenone and mixtures thereof, most preferably selected from the group consisting of phenoxyethanol and ethylhexylglycerin and mixtures thereof. When present, the preservative or preservative aid is preferably in an amount of 0.01 to 2% by weight, more preferably in an amount of 0.05 to 1.5% by weight, most preferably in an amount of 0.1 to 1.0% by weight, for example in an amount of 0.1 to 0.5% by weight, based on the total weight of the composition. 【0066】 In another advantageous embodiment, the sunscreen composition according to the present invention does not contain any parabens, benzethonium chloride, piroctone olamine, lauroyl arginate, methylisothiazolinone, chloromethylisothiazolinone, bronopol, benzalkonium chloride, formaldehyde - releasing compounds, salicylic acid, triclosan, DMDM hydantoin, chlorphenesin and IPBC (iodopropynyl butylcarbamate). 【0067】 In a further advantageous embodiment, the sunscreen composition of the present invention further comprises one or more of glycerol or polyhydroxystearic acid and mixtures thereof. 【0068】 In a further advantageous embodiment, the sunscreen composition of the present invention further comprises a thickener, preferably a gum such as xanthan gum or Caesalpinia spinosa gum or a polyacrylate such as polyacrylate crosspolymer - 6 and mixtures thereof. 【0069】 The sunscreen composition according to the present invention generally has a pH in the range of 3 to 10, preferably in the range of 4 to 8, and most preferably in the range of 4 to 7. The pH can be easily adjusted as needed using a suitable acid, such as citric acid, or a base, such as NaOH, according to standard methods in the art. 【0070】 The sunscreen composition according to the present invention may further contain one or more skin softeners that soothe and soften the skin. As an example, the skin softener can be dicaprylyl carbonate or dibutyl adipate. Further skin softeners are silicones (dimethicone, cyclomethicone), vegetable oils (grape seed, sesame seed, jojoba, etc.), butters (cocoa butter, shea butter), alcohols (stearyl alcohol, cetyl alcohol), and petrolatum derivatives (petrolatum, mineral oil). 【0071】 In yet another aspect, the sunscreen composition of the present invention may include potassium cetyl phosphate as an emulsifier and / or one or more of dibutyl adipate, dicaprylyl carbonate, and stearoyl alcohol. 【0072】 In yet another aspect, the sunscreen composition of the present invention may contain an acrylate copolymer, such as acrylate / C10-30 alkyl acrylate crosspolymer, in an amount of more preferably 0.05 to 1% by weight, more preferably 0.1 to 0.5% by weight, based on the total weight of the sunscreen composition. 【0073】 In another aspect, the sunscreen composition of the present invention may contain one or more fragrances selected from limonene, citral, linalool, alpha-isomethyl ionone, geraniol, citronellol, 2-isobutyl-4-hydroxy-4-methyltetrahydropyran, 2-tert-pentylcyclohexyl acetate, 3-methyl-5-phenyl-1-pentanol, 7-acetyl-1,1,3,4,4,6-hexamethyltetralin, adipic acid diester, cinnamal, amyl salicylate, alpha-amylcinnamaldehyde, alpha-methyl ionone, butylphenylmethyl propionaldehyde, cinnamal, amylcinnamyl alcohol, anis alcohol, benzoin, benzyl alcohol, benzyl benzoate, benzyl cinnamate, benzyl salicylate, bergamot oil, bitter orange oil, butylphenylmethyl propionaldehyde, cardamom oil, cedrol, cinnamal, cinnamyl alcohol, citronellyl methylcrotonate, citrus oil, coumarin, diethyl succinate, ethyl linalool, eugenol, Evernia furfuracea extract, Usnea barbata extract, farnesol, guaiacwood oil, hexyl cinnamal, hexyl salicylate, hydroxycitronellal, lavender oil, lemon oil, linalyl acetate, mandarin oil, PCA menthyl, methyl heptenone, nikkuzu oil, rosemary oil, sweet orange oil, terpineol, tonka bean oil, triethyl citrate, vanillin. 【0074】 In another aspect, the sunscreen composition of the present invention may contain at least one salt of 2-phenylbenzimidazole-5-sulfonic acid. 【0075】 In another aspect, the composition may have an SPF of at least 20, preferably at least 30. 【0076】 The sunscreen composition of the present invention may have a surprisingly low total amount of UV filter. 【0077】 According to the present invention, advantageously, the sunscreen composition is present in the form of an emulsion or a dispersion, preferably in the form of an emulsion, more preferably in the form of an O / W emulsion. 【0078】 When the sunscreen composition of the present invention is in the form of an O / W emulsion, the preparation advantageously comprises one or more O / W emulsifiers selected from glyceryl citrate stearate, glyceryl stearate (self-emulsifying type), stearic acid, stearate, polyglyceryl-3-methylglucose distearate, sodium cetearyl sulfate, potassium cetyl phosphate, polyglyceryl-10 stearate and sodium stearyl glutamate. 【0079】 According to the present invention, advantageously, these O / W emulsifiers of the present invention may be present in the preparation at a concentration of 0.001 to 10% by weight, preferably 0.1 to 7% by weight, based on the total weight of the preparation. 【0080】 According to the present invention, preferably, the preparation comprises potassium cetyl phosphate as an emulsifier. 【0081】 According to the present invention, more advantageously, the preparation comprises cetyl alcohol, stearyl alcohol and / or glyceryl stearate. 【0082】 According to the present invention, advantageously, the preparation of the present invention does not contain polyethylene glycol, polyethylene glycol ether and polyethylene glycol ester (so-called PEG derivatives). 【0083】 The preparation of the present invention may advantageously contain a humectant. A humectant is a compound or a mixture of compounds that imparts to the cosmetic preparation the quality of reducing the water loss of the stratum corneum (also referred to as transepidermal water loss (TEWL)) and / or having a favorable effect on the hydration of the stratum corneum after the cosmetic preparation is applied or spread on the skin surface. 【0084】 Non-limiting examples of humectants advantageous for use in the present invention include glycerol, lactic acid and / or lactate, especially sodium lactate, butylene glycol, propylene glycol, biosaccharide gum-1, winged bean, ethylhexylglycerin, pyrrolidone carboxylic acid, and urea. In particular, the use of polymeric humectants from the group of polysaccharides that are water-soluble and / or water-swellable and / or gellable by the force of water is even more advantageous. Particularly advantageous are, for example, hyaluronic acid, chitosan, and / or fucose-rich polysaccharides, and the fucose-rich polysaccharide is registered with the Chemical Abstracts registration number 178463-23-5 and is available, for example, from the company SOLABIA S.A. under the name Fucogel® 1000. The humectant can also be advantageously used, for example, as an anti-wrinkle component to protect against changes to the skin of the type that occur with skin aging. 【0085】 Although not essential, advantageously, the cosmetic preparation of the present invention may further comprise, for example, a filler that further improves the sensory and cosmetic properties of the formulation, such as an effect of evoking or enhancing a velvety or silky skin feel. Advantageous fillers in the context of the present invention are starch and starch derivatives (such as tapioca starch, phosphate-crosslinked starch, aluminum or sodium octenyl succinate starch, etc.), pigments that exhibit essentially no UV filtering effect nor coloring effect (such as boron nitride, etc.) and / or Aerosils® (CAS No. 7631-86-9) and / or talc and / or polyethylene, nylon, and dimethylsilylated silica. 【0086】 [[ID=*]] As an advantageous embodiment of the preparation of the present invention, an embodiment is also included in which the preparation contains one or more oils selected from dibutyl adipate, dicaprylyl carbonate, alkyl (C12 - 15) benzoate, dibutyl sebacate, diisopropyl sebacate, dicaprylyl carbonate, dialkyl (C12 - 13) tartrate, butyl octyl salicylate, diethylhexyl malonate, hydrogenated castor oil dimer acid ester, triheptanoin, alkyl (C12 - 13) lactate, alkyl (C16 - 17) benzoate, propyl heptyl caprylate, glyceryl tri(caprylate / caprate), diethylhexyl naphthalenedicarboxylate, octyldodecanol, and ethylhexyl coconut oil fatty acid. 【0087】 According to the present invention, preparations containing dibutyl adipate, dicaprylyl carbonate, and / or alkyl (C12 - 15) benzoate are preferred. 【0088】 The aqueous phase of the preparation according to the invention advantageously contains conventional cosmetic adjuvants, such as, in each case individually or in combination, alcohols, especially those with a low carbon number, preferably ethanol and / or isopropanol or polyols with a low carbon number and their ethers, preferably propylene glycol, glycerol, electrolytes, self-tanning agents and, in addition to these, especially one or more thickeners, advantageously selected from the group consisting of silicon dioxide, aluminium silicate, polysaccharides and / or their derivatives, such as hyaluronic acid, xanthan gum, hydroxypropylmethylcellulose, especially preferably thickeners from the group of polyacrylates, preferably polyacrylates of the group known as Carbopol (examples of which are Carbopol types 980, 981, 1382, 2984 and 5984). According to the invention, further advantageous thickeners are those having the INCI name of acrylate / acrylic acid C10-30 alkyl crosspolymer (for example, Pemulen TR 1, Pemulen TR 2, Carbopol 1328 from NOVEON) and also Aristoflex AVC (INCI: ammonium acryloyldimethyltaurate / VP copolymer) and Simugel NS (INCI: hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer and squalane and polysorbate 60). 【0089】 In this regard, according to the invention, it is preferred that the preparation contains xanthan gum, crosslinked acrylate / alkyl (C10-30) acrylate polymer, (hydroxyethyl acrylate / acryloyldimethyltaurine Na) copolymer and squalane and polysorbate 60 and / or vinylpyrrolidone / hexadecene copolymer. 【0090】 According to the invention, an amount of at least 2.5% by weight of glycerol, based on the total weight of the preparation, is particularly advantageous. 【0091】 According to the present invention, it is advantageous for the preparation to contain one or more alkanediols from the group of 1,2-pentanediol, 1,2-hexanediol, 1,2-octanediol, 1,2-decanediol, 2-methyl-1,3-propanediol. 【0092】 According to the present invention, it is advantageous for the preparation of the present invention to contain ethanol, phenoxyethanol and / or ethylhexylglycerin. 【0093】 According to the present invention, it is advantageous for the preparation of the present invention to contain a film-forming agent. The film-forming agent in the meaning of the present invention is various kinds of substances and is characterized by the following properties. When the film-forming agent is dissolved in water or another suitable solvent and this solution is then applied to the skin, the film-forming agent forms a film after the solvent has evaporated, and this film basically serves to fix a light-shielding substance to the skin and enhance the water resistance of the product. 【0094】 It is particularly advantageous to select a film-forming agent from the group of polymers based on polyvinylpyrrolidone (PVP). 【0095】 For example, copolymers of vinylpyrrolidone such as (PVP / hexaadecene) copolymer and (PVP / eicosene) copolymer (which are available from GAF Chemicals Corporation under the trade names Antaron V216 and Antaron V220) are particularly preferred. 【0096】 Similarly, additional polymer film formers such as, for example, sodium polystyrene sulfonate (which is available under the trade name Flexan 130 from National Starch and Chemical Corp.) and / or polyisobutene (which is available under the trade name Rewopal PIB1000 from Rewo) are also advantageous. Examples of further suitable polymers are polyacrylamide (Seppigel 305), polyvinyl alcohol, PVP, (PVP / VA) copolymers, polyglycols, (acrylate / octylacrylamide) copolymers (Dermacryl 79). It is likewise advantageous to use hydrogenated castor oil dimer dilinoleic acid (CAS 646054-62-8, INCI hydrogenated castor oil dimer dilinoleic acid) (which is available under the name Risocast DA-H from Kao Alcohol Industries Co., Ltd.) or PPG-3 benzyl ether myristate (CAS 403517-45-3) (which is available under the trade name Crodamol STS from Croda Chemicals). 【0097】 According to the invention, the preparation of the invention is used as an ultraviolet protection composition in addition to protecting the skin from aging, in particular from skin aging caused by UV rays. 【0098】 The following examples are provided to further illustrate the compositions and effects of the invention. These examples are merely illustrative and are not intended to limit the scope of the invention in any way. 【0099】 [Examples] [In Vitro UV Model] The microbial strains used in this test are listed below and their cultures are reconstituted according to the instructions of the collection center (ATCC American Type Culture Collection, USA), the supplier of the reference strains: ·Cutibacterium acnes ATCC 11827) · Staphylococcus epidermidis ATCC 12228 【0100】 A 96-well plate was filled with the recommended culture medium (140 μL), and the calibrated inoculum (20 μL) of each bacterial strain was added to reach a final concentration of 1.106 colony-forming units (cfu / mL) per milliliter. After contamination, the sample (40 μL; 20% volume / volume) was added three times and mixed well to ensure a uniform distribution. The plate was then subjected to UV stress via a UV irradiation device system (BioSun, Vilber Lourmat, France) according to the UV doses shown in Table 1 below. At the specific UV doses selected, the microbial population survived between 10 and 49%. 【0101】 【Table 1】 【0102】 The count of the C. acnes microbial population was performed by plating aliquots on agar plates and was done three times. The growth rate of the C. acnes colonies was used to determine the reduction rate of the microbial population. The control performed during this experiment was a culture medium containing bacteria incubated without UV stress. The microbial population count was expressed in cfu / mL and was taken as the average of the three experiments performed. 【0103】 For sample collection and microbial population count, aliquots of the contaminated sample of S. epidermidis were serially diluted in a culture medium on a 96-well plate containing triphenyltetrazolium (four 10-fold dilutions followed by eight 2-fold dilutions, three times). The plate was then incubated at 37 °C ± 2.5 °C for 48 hours, taking into account the type of respiration of each strain. After 48 hours under optimal growth conditions, the last dilution showing pink color (triphenyltetrazolium) or turbidity indicated the contamination rate and enabled the determination of the reduction rate of the microbial population (most probable number (MNP) method). 【0104】 The usage levels of the single UV filter and combinations in the test complexes were calculated to obtain in vitro SPF prediction values of 5.75 + / - 0.15 and 23.45 + / - 0.65, respectively (Table 2). The UV filters were mixed in two oils, Tegosoft XC (55%) and Miglyol 829 Eco (up to ad100%), and the emulsifier Tween 80 (5%). 【0105】 The effects of the selected UV irradiation conditions on the survival of the microbial populations of C. acnes and S. epidermidis in the presence of 20% volume / volume of test samples 1 - 10 were compared with the corresponding strains in the absence of samples 1 - 10 and without exposure to UV irradiation (control). The results after 4 hours of incubation were compared with the bacterial populations incubated without UV stress (determination of the survival of the bacterial populations) and expressed as the percentage of survival. The relative percentage of protection (%) was calculated from the difference between the survival percentages of the samples and the vehicle control (with UV irradiation) divided by the survival percentage of the vehicle control. As a result, the survival of each microbial population and the relative protection rate by the UV filter were calculated and summarized in Table 2. 【0106】 【Table 2】 【0107】 【Table 3】 【0108】 The inventors tested strain ATCC 11827 of C. acnes, a opportunistic pathogen that may be associated with acne-prone skin, of phylotype IA. Selected UV filters, such as combinations of BMDBM, EHS and octocrylene and octocrylene, EHS and EHT and EHT, BEMT and BDMBM, reduced the population of C. acnes in the skin microbiota during UV irradiation while maintaining or further growing the commensal bacterium Staphylococcus epidermidis, worsening the progression of acne-prone skin.

Claims

[Claim 1] A method for reducing C. acnes in the skin microbiome of a person in need thereof, comprising the step of applying an effective amount of a sunscreen composition containing one or more UV filters selected from the group consisting of octocrylene, ethylhexyl salicylate, and butyl methoxydibenzoylmethane to the outer surface of a human body having a skin microbiome containing C. acnes and optionally Staphylococcus epidermidis, before the outer surface is exposed to UV radiation. [Claim 2] The method according to claim 1, wherein Staphylococcus epidermidis is also protected from the harmful effects of UV radiation. [Claim 3] The method according to claim 1 or 2, wherein the reduction of C. acnes is characterized by a reduction in survival rate of at least -10% compared to a control after exposure to UV radiation. [Claim 4] The method according to claim 1 or 2, wherein protection of Staphylococcus epidermidis is characterized by an increase in survival rate of at least 20% compared to a control after exposure to UV radiation. [Claim 5] The method according to claim 1 or 2, wherein the amount of the one or more UV filters in the sunscreen composition is selected such that the sunscreen composition exhibits an SPF of at least 20. [Claim 6] The method according to claim 1, wherein the Cutibacterium acnes is strain C. acnes ATCC 11827. [Claim 7] The method according to claim 1 or 2 for treating or preventing skin dysbiosis after UV exposure. [Claim 8] The method according to claim 1 or 2, wherein the amount of butyl methoxydibenzoylmethane is selected in the range of 0.1% to 10% by weight based on the total weight of the sunscreen composition. [Claim 9] The method according to claim 1 or 2, wherein the amount of octocrylene is selected in the range of 0.1% to 10% by weight based on the total weight of the sunscreen composition. [Claim 10] The method according to claim 1 or 2, wherein the amount of ethylhexyl salicylate in the sunscreen composition is in the range of 0.1% to 20% by weight, based on the total weight of the sunscreen composition. [Claim 11] A composition comprising octocrylene, ethylhexyl salicylate, and / or butyl methoxydibenzoylmethane for use in the prevention or treatment of acne. [Claim 12] A sunscreen composition for use in the prevention or treatment of acne, comprising one or more UV filters selected from the group consisting of octocrylene, ethylhexyl salicylate, and butyl methoxydibenzoylmethane. [Claim 13] The amount of butyl methoxydibenzoylmethane is selected in the range of 0.1% to 10% by weight based on the total weight of the sunscreen composition, the sunscreen composition according to claim 12 for use in the prevention or treatment of acne. [Claim 14] The amount of octocrylene is selected in the range of 0.1% to 10% by weight based on the total weight of the sunscreen composition, the sunscreen composition for use in preventing or treating acne according to claim 12 or 13. [Claim 15] The sunscreen composition for use in the prevention or treatment of acne according to claim 12 or 13, wherein the amount of ethylhexyl salicylate in the sunscreen composition is in the range of 0.1% by weight to 20% by weight, based on the total weight of the sunscreen composition.