Chinese herbal medicine prescriptions and their preparation methods

Abstract

A traditional Chinese medicine formula and its preparation method relate to the field of disinfection. The traditional Chinese medicine formula comprises, by weight, 8-12 parts rose hydrosol, 1-3 parts mint hydrosol, 28-42 parts traditional Chinese medicine extract, 1-3 parts chelated platinum ions, 1-3 parts chelated selenium ions, and 4-10 parts bacteriostatic agent. The preparation method includes the steps of adding the rose hydrosol and mint hydrosol to the traditional Chinese medicine extract to obtain a stock solution, diluting the stock solution, adding the chelated platinum ions, chelated selenium ions, and bacteriostatic agent, and stirring uniformly to obtain the traditional Chinese medicine formula. The prepared solution effectively kills bacteria, viruses, and fungi, with excellent killing effect, without causing any adverse effects to the human body.

Description

[Technical Field]

[0001] The present invention relates to the technical field of disinfection, specifically to a traditional Chinese medicine formula and its preparation method. [Background technology]

[0002] With the development of society and the improvement of people's living standards, the harmful effects of harmful microorganisms such as bacteria on human health and the living environment are becoming increasingly important, making it increasingly important to actively search for antibacterial materials that are effective for a long period of time.

[0003] Herbal disinfectants are less likely to develop drug resistance during use, and have properties such as antibacterial and anti-inflammatory properties, low toxicity, and low or no residual properties, while also being able to effectively inhibit and kill pathogenic microorganisms. This is because herbal medicines contain multiple biologically active ingredients that can interfere with multiple metabolic processes in pathogenic microorganisms, such as energy conversion, DNA, and RNA. Herbal disinfectants are easy to apply and popularize because the preparation process and equipment are relatively simple, they are available from a wide range of sources, and are relatively low cost.

[0004] Polymeric antibacterial agents have a high density of active functional groups and exhibit excellent antibacterial activity. They can kill bacteria through direct contact with them without releasing active substances. They also maintain stable antibacterial activity over a long period of time, have low residual toxicity, and are highly safe. Based on the active functional groups, polymeric antibacterial agents are primarily classified as quaternary ammonium salts, quaternary phosphonium salts, pyridinium salts, haloamines, guanidine salts, chitosan, etc. Among these, quaternary ammonium salts are typical and representative of polymeric antibacterial agents, characterized by a positively charged quaternary ammonium nitrogen ion at the center of their structure, surrounded by four substituents and counter anions. The antibacterial principle of quaternary ammonium salt-based polymeric antibacterial agents is that the long carbon chain groups attached to the quaternary ammonium nitrogen ion puncture the lipid cell membrane of bacteria, thereby achieving bacterial killing.

[0005] Patent Document 1 discloses a herbal disinfectant. This disinfectant is obtained by homogeneously mixing a compound of Chinese herbs, such as Rhubarb, Artemisia Root, Paeonol, Snake Seeds, Rhizome, Sophora Radix, and Chinese Gallnut, adding water to the resulting mixture, boiling it, and then steaming it with the aid of ultrasound, followed by concentrating and precipitating it. This disinfectant has excellent inhibitory effects against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. Furthermore, its stability is minimally affected by temperature, demonstrating its clear disinfecting effect in clinical settings. However, in actual application, it has been found that the bacteriostatic effect of this disinfectant is not ideal, limiting its wide applicability. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] Chinese Patent Application No. 201810634700.4 Summary of the Invention [Problem to be solved by the invention]

[0007] In order to overcome the deficiencies of the prior art, the object of the present invention is to provide a Chinese herbal medicine formula and a method for preparing the same, which has good product quality stability, is easy to obtain, is inexpensive, has excellent bactericidal effect, and does not cause any harm to the human body. [Means for solving the problem]

[0008] In order to achieve the above object, the present invention employs the following technical means.

[0009] The herbal prescription consists of, by weight, 8-12 parts rose hydrosol, 1-3 parts mint hydrosol, 28-42 parts herbal extract, 1-3 parts chelated platinum ion, 1-3 parts chelated selenium ion, and 4-10 parts bacteriostatic agent.

[0010] The method for preparing the bacteriostatic agent includes the following steps:

[0011] (1) Bentonite is calcined, cooled to room temperature, and crushed. It is then dispersed in an ethanol solution and the pH is adjusted with hydrochloric acid. (3-mercaptopropyl)triethoxysilane is then added and the mixture is stirred to react. The resulting product is then centrifuged, washed, and dried to obtain thiolated bentonite.

[0012] (2) Dodecafluoroheptyl methacrylate and (methacryloyloxyethyl)trimethylammonium chloride are added to N,N-dimethylformamide and stirred uniformly to obtain a mixed solution. Then, thiolated bentonite is dispersed in the mixed solution and ultrasonically dispersed, a photoinitiator is added, and the mixture is reacted by irradiating with ultraviolet light. The product is then centrifuged, washed with isopropanol, and dried to obtain the bacteriostatic agent.

[0013] Preferably, in step (1), the concentration of the aqueous ethanol solution is 50 to 75 wt %, and the pH is adjusted to 3 to 5.

[0014] Preferably, in step (1), the weight ratio of bentonite, the aqueous ethanol solution, and (3-mercaptopropyl)triethoxysilane is 10:30-50:2-5.

[0015] Preferably, in step (1), the calcination conditions are 150 to 300° C. for 1 to 2 hours, and the stirring reaction conditions are 30 to 45° C. in a N 2 atmosphere for 5 to 8 hours.

[0016] Preferably, in step (2), the weight ratio of dodecafluoroheptyl methacrylate, (methacryloyloxyethyl)trimethylammonium chloride, N,N-dimethylformamide, and thiolated bentonite is 4-7:1-3:30-50:10.

[0017] Preferably, in step (2), the photoinitiator is one or more of benzoin dimethyl ether, benzoin ethyl ether, benzoin butyl ether.

[0018] Preferably, in step (2), the ultrasonic dispersion time is 15 to 30 min, the main wavelength of the ultraviolet light is 360 to 365 nm, and the ultraviolet light intensity is 45 to 75 mW / cm. 2 The irradiation time is 60 to 90 minutes, and the reaction temperature is 35 to 45°C.

[0019] Preferably, the method for preparing the herbal extract comprises the following steps: 1-2 parts safflower, 8-12 parts peach kernel, 8-12 parts forsythia, 8-13 parts chrysanthemum, 7-13 parts perilla, 18-25 parts platycodon, 8-11 parts star anise, 9-14 parts serrano rhizome, 7-12 parts cuckoo root, 10-12 parts sophora rhizome, 10-12 parts white atractylodes, 9-13 parts green peel, 10-12 parts clove fragrans, 4-6 parts Sichuan pepper, and 13-17 parts tea leaves are weighed into a reaction vessel, added to the vessel, and distilled at 94-97°C for 1.5-3 hours to obtain the herbal extract.

[0020] The present invention also claims a method for preparing the above-mentioned herbal formula, which comprises the steps of: adding rose hydrosol and mint hydrosol to a herbal extract to obtain a stock solution; diluting the stock solution, adding chelated platinum ions, chelated selenium ions, and a bacteriostatic agent, and stirring uniformly to obtain the herbal formula.

[0021] Preferably, the dilution ratio of the stock solution is 10 to 20 times. [Effects of the Invention]

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] Safflower: Improves menstrual irregularities by promoting blood circulation and relieves pain by eliminating blood stasis. This contributes to the therapeutic effects on menstrual irregularities, menstrual pain, abnormal discharge, chest pain, abdominal pain due to congestion, chest and flank pain, injuries, and swelling and pain from ulcers.

[0024] Peach kernel: It has the effect of promoting blood circulation to relieve blood congestion, moistening the intestines to promote bowel movements, suppressing coughs, and improving asthma. It is effective for menstrual irregularities and menstrual pain, intraperitoneal lumps, lung abscesses and appendicitis, trauma, constipation due to dry intestines, coughs, and asthma.

[0025] Forsythia: Helps clear heat, detoxify, eliminate lumps, and relieve swelling and edema. It is used to treat fever, erysipelas, rashes, abscesses, swelling, scrofula, and difficulty urinating.

[0026] Chrysanthemum: Helps relieve the initial symptoms of colds, clears fever, improves eyesight, and detoxifies. It is used to treat headaches, dizziness, bloodshot eyes, chest fever, boils, sores, and swelling.

[0027] Perilla: The leaves are diaphoretic, antitussive, aromatic stomachic, diuretic, analgesic, sedative, and detoxifying, helping to treat colds. The stems have a calming effect and stabilize the fetus. The seeds are antitussive, expectorant, improve asthma, and relieve stress.

[0028] Platycodon: Improves lung function, soothes the throat, expels phlegm, and helps drain pus. This is used in cases of excessive cough and phlegm, shortness of breath, sore throat, hoarseness, drainage of pus from lung abscesses, and ulcers that are filled with pus but not burst.

[0029] Star anise: Has a warming effect on the body and a pungent taste. It has the effect of warming the body and expelling cold, as well as regulating the flow of qi and relieving pain. It is used to treat symptoms such as vomiting due to cold, abdominal pain due to cold, lower back pain associated with kidney deficiency, and dry and wet athlete's foot.

[0030] Wood Fragrance: Improves the flow of energy, relieves pain, regulates the stomach and intestines, and aids digestion. Effective for chest and stomach distension and pain, diarrhea, heaviness after a stomach ulcer, indigestion, and loss of appetite.

[0031] Cuckoo: Helps restore vital energy, regulates the stomach and intestines, removes impurities, and expels fluids. It is used to treat colds, heat and dampness, headaches, chest and stomach bloating, vomiting, diarrhea, malaria, dysentery, and bad breath.

[0032] Soujutsu: Regulates the stomach and intestines, removes dampness, relieves depression, and removes impurities. It is used to treat digestive disorders caused by fluid retention, drowsiness due to fatigue, stomach and abdominal bloating, loss of appetite, vomiting, diarrhea, dysentery, malaria, phlegm, edema, seasonal influenza, pain and numbness caused by cold and humidity, weakness of the lower limbs, and night blindness.

[0033] Atractylodes Root: Improves gastrointestinal function, eliminates dampness, regulates the stomach and intestines, and stabilizes the fetus. It is used to treat poor digestion, loss of appetite, fatigue and shallow breathing, abdominal distension due to qi deficiency, diarrhea, phlegm, edema, jaundice, pain and numbness due to dampness, difficulty urinating, dizziness, spontaneous sweating, and pregnancy disorders.

[0034] Qingpi: Removes stagnation of liver qi and relieves gastrointestinal congestion. It is effective for chest and flank swelling and pain, hernia, breast tuberculosis, mastitis, and abdominal pain caused by overeating.

[0035] Cingoliferous: Warms the stomach and intestines, warms the kidneys, and lowers Qi. It is used to treat hiccups, vomiting, nausea, diarrhea, chest and abdominal pain associated with cold, lumps in the abdomen and flanks, hernias, and psoriasis.

[0036] Sichuan pepper: warms the stomach and intestines and removes cold. It also helps to dehumidify, relieve pain, kill insects, and remove fishy odors. It is used to treat indigestion and water retention, chest and abdominal pain associated with cold, vomiting, belching, coughing, and irritation, pain and numbness caused by cold and dampness, diarrhea, dysentery, colic, toothache, ascariasis, pinworm infestation, genital itching, and scabies.

[0037] Tea leaves: Helps to clear heat from the head and eyes, relieve dry mouth, expectorate, aid digestion, diuretic, and detoxify. It is used to treat headaches, blurred vision, excessive sleepiness, irritability and dry mouth, indigestion and phlegm congestion, malaria, and diarrhea.

[0038] 1) This invention discloses a traditional Chinese medicine prescription. The care solution, formulated from plant ingredients with anti-inflammatory, antioxidant, allergy-preventing, infection-reducing, and immune-boosting effects, a complex antibacterial component, and chelated platinum ions and chelated selenium ions that enhance the performance of herbal medicines through catalytic action, has excellent bactericidal and bacteriostatic effects, and can also effectively moisturize and protect the skin. Furthermore, it can be applied in a spray format, making it convenient and quick.

[0039] 2) The present invention provides a bacteriostatic agent. First, thiolated bentonite is obtained by organically modifying bentonite with (3-mercaptopropyl)triethoxysilane. This effectively improves the dispersibility of bentonite in herbal medicine extracts, preventing the bentonite from flocculating and settling within the medicine. Next, dodecafluoroheptyl methacrylate and (methacryloyloxyethyl)trimethylammonium chloride are grafted onto the thiolated bentonite through a thiol-ene reaction under ultraviolet irradiation to obtain a bacteriostatic agent. (Methacryloyloxyethyl)trimethylammonium chloride can change the permeability of bacterial membranes, killing the bacteria. It also has good surfactant activity, concentrating on the bacterial surface and affecting bacterial metabolism. It can also denature proteins and inactivate bacterial enzymes, making it safe, non-toxic, and non-irritating to the human body. Furthermore, it does not induce drug resistance in bacteria, resulting in excellent antibacterial activity. Furthermore, dodecafluoroheptyl methacrylate has excellent hydrophobicity, which can prevent the initial adhesion of bacteria and proteins and inhibit the formation of biofilms, thereby exerting an inhibitory and killing effect on bacteria in cooperation with (methacryloyloxyethyl)trimethylammonium chloride. DETAILED DESCRIPTION OF THE INVENTION

[0040] In order to clarify the objectives, technical means and advantages of the present invention, the present invention will be described in more detail below using examples. It should be understood that the specific examples described here are merely for the purpose of illustrating the present invention and are not intended to limit the present invention.

[0041] Unless otherwise specified, all chemical reagents and materials used in the present invention were either commercially available or synthesized from commercially available raw materials.

[0042] Bentonite with a particle size of 4000-5000 mesh was purchased from Haoyuan Bentonite Factory, Fangzi District, Weifang City.

[0043] Both rose hydrosol and mint hydrosol were purchased from Guangzhou Zhonghe International Biotechnology Co., Ltd.

[0044] Chelated platinum ions and chelated selenium ions were purchased from Yatian Technology Group.

[0045] The present invention will be further described below with reference to specific examples. [Example]

[0046] The preparation method of the herbal formula included the following steps:

[0047] (1) 10 g of bentonite was calcined at 300°C for 2 hours, cooled to room temperature, and crushed. The mixture was dispersed in 50 g of a 70 wt% aqueous ethanol solution and the pH was adjusted to 5 with hydrochloric acid. 5 g of (3-mercaptopropyl)triethoxysilane was then added, and the mixture was stirred at 45°C for 8 hours in a N2 atmosphere to react. The product was then centrifuged, washed, and dried to obtain thiolated bentonite.

[0048] (2) 7 g of dodecafluoroheptyl methacrylate and 3 g of (methacryloyloxyethyl)trimethylammonium chloride were added to 50 g of N,N-dimethylformamide and stirred uniformly to obtain a mixed solution. Then, 10 g of thiolated bentonite was dispersed in the mixed solution, and ultrasonic dispersion was performed for 30 minutes. Benzoin butyl ether was added, and the mixture was then dispersed at a dominant wavelength of 365 nm and an intensity of 60 mW / cm. 2 The mixture was irradiated with ultraviolet light at 45°C for 90 minutes, and the product was centrifuged, washed with isopropanol, and dried to obtain the bacteriostatic agent.

[0049] (3) 2g of safflower, 10g of peach kernel, 10g of forsythia, 11g of chrysanthemum, 10g of shiso, 22g of bellflower, 9g of star anise, 12g of wood spices, 8g of cuckoo root, 11g of soju, 11g of white atractylodes, 11g of ginger peel, 11g of clove spices, 5g of Sichuan pepper, and 16g of tea leaves were weighed and placed in a reaction vessel. 500g of deionized water was added, and the mixture was distilled at 96°C for 2.5 hours to obtain a Chinese herbal extract.

[0050] (4) 12g of rose hydrosol and 3g of mint hydrosol were added to 42g of herbal extract to obtain a stock solution, which was then diluted 20 times. 3g of chelated platinum ion, 3g of chelated selenium ion, and 10g of bacteriostatic agent were added and stirred uniformly to obtain the herbal formula. [Example]

[0051] The preparation method of the herbal formula included the following steps:

[0052] (1) 10 g of bentonite was calcined at 200°C for 1.5 hours, cooled to room temperature, and crushed. The mixture was dispersed in 40 g of a 70 wt% aqueous ethanol solution and the pH was adjusted to 4 with hydrochloric acid. 4 g of (3-mercaptopropyl)triethoxysilane was then added, and the mixture was stirred at 35°C for 7 hours in a N2 atmosphere to react. The product was then centrifuged, washed, and dried to obtain thiolated bentonite.

[0053] (2) 5 g of dodecafluoroheptyl methacrylate and 2 g of (methacryloyloxyethyl)trimethylammonium chloride were added to 40 g of N,N-dimethylformamide and stirred uniformly to obtain a mixed solution. Then, 10 g of thiolated bentonite was dispersed in the mixed solution, and ultrasonic dispersion was performed for 20 minutes. Benzoin butyl ether was added, and the mixture was then dispersed at a dominant wavelength of 365 nm and an intensity of 60 mW / cm. 2 The mixture was irradiated with ultraviolet light at 40°C for 75 minutes, and the product was centrifuged, washed with isopropanol, and dried to obtain the bacteriostatic agent.

[0054] (3) 2g of safflower, 10g of peach kernel, 10g of forsythia, 11g of chrysanthemum, 10g of shiso, 22g of bellflower, 9g of star anise, 12g of wood spices, 8g of cuckoo root, 11g of soju, 11g of white atractylodes, 11g of ginger peel, 11g of clove spices, 5g of Sichuan pepper, and 16g of tea leaves were weighed and placed in a reaction vessel. 500g of deionized water was added, and the mixture was distilled at 96°C for 2.5 hours to obtain a Chinese herbal extract.

[0055] (4) 10g of rose hydrosol and 2g of mint hydrosol were added to 35g of herbal extract to obtain a stock solution, which was then diluted 20 times. 2g of chelated platinum ion, 2g of chelated selenium ion, and 7g of bacteriostatic agent were added and stirred uniformly to obtain the herbal formula. [Example]

[0056] The preparation method of the herbal formula included the following steps:

[0057] (1) 10 g of bentonite was calcined at 150°C for 1 hour, cooled to room temperature, and crushed. The mixture was dispersed in 30 g of a 70 wt% aqueous ethanol solution and the pH was adjusted to 3 with hydrochloric acid. 2 g of (3-mercaptopropyl)triethoxysilane was then added, and the mixture was stirred at 30°C for 5 hours in a N2 atmosphere to react. The product was then centrifuged, washed, and dried to obtain thiolated bentonite.

[0058] (2) 4 g of dodecafluoroheptyl methacrylate and 1 g of (methacryloyloxyethyl)trimethylammonium chloride were added to 30 g of N,N-dimethylformamide and stirred uniformly to obtain a mixed solution. Then, 10 g of thiolated bentonite was dispersed in the mixed solution, and ultrasonic dispersion was performed for 15 minutes. Benzoin butyl ether was added, and the mixture was then dispersed at a dominant wavelength of 365 nm and an intensity of 60 mW / cm. 2 The mixture was irradiated with ultraviolet light at 35°C for 60 minutes, and the product was centrifuged, washed with isopropanol, and dried to obtain a bacteriostatic agent.

[0059] (3) 2g of safflower, 10g of peach kernel, 10g of forsythia, 11g of chrysanthemum, 10g of shiso, 22g of bellflower, 9g of star anise, 12g of wood spices, 8g of cuckoo root, 11g of soju, 11g of white atractylodes, 11g of ginger peel, 11g of clove spices, 5g of Sichuan pepper, and 16g of tea leaves were weighed and placed in a reaction vessel. 500g of deionized water was added, and the mixture was distilled at 96°C for 2.5 hours to obtain a Chinese herbal extract.

[0060] (4) 8g of rose hydrosol and 1g of mint hydrosol were added to 28g of herbal extract to obtain a stock solution, which was then diluted 20 times. 1g of chelated platinum ion, 1g of chelated selenium ion, and 4g of bacteriostatic agent were added and the mixture was stirred uniformly to obtain the herbal formula.

[0061] (Comparative Example 1) The preparation method of the herbal formula included the following steps:

[0062] (1) 10 g of bentonite was calcined at 300°C for 2 hours, cooled to room temperature, and crushed. The mixture was dispersed in 50 g of a 70 wt% aqueous ethanol solution and the pH was adjusted to 5 with hydrochloric acid. 5 g of (3-mercaptopropyl)triethoxysilane was then added, and the mixture was stirred at 45°C for 8 hours in a N2 atmosphere to react. The product was then centrifuged, washed, and dried to obtain thiolated bentonite.

[0063] (2) 7 g of dodecafluoroheptyl methacrylate was added to 50 g of N,N-dimethylformamide and stirred uniformly to obtain a mixed solution. Then, 10 g of thiolated bentonite was dispersed in the mixed solution, and ultrasonic dispersion was performed for 30 minutes. Benzoin butyl ether was added, and the mixture was then dispersed at a dominant wavelength of 365 nm and an intensity of 60 mW / cm. 2 The mixture was irradiated with ultraviolet light at 45°C for 90 minutes, and the product was centrifuged, washed with isopropanol, and dried to obtain the bacteriostatic agent.

[0064] (3) 2g of safflower, 10g of peach kernel, 10g of forsythia, 11g of chrysanthemum, 10g of shiso, 22g of bellflower, 9g of star anise, 12g of wood spices, 8g of cuckoo root, 11g of soju, 11g of white atractylodes, 11g of ginger peel, 11g of clove spices, 5g of Sichuan pepper, and 16g of tea leaves were weighed and placed in a reaction vessel. 500g of deionized water was added, and the mixture was distilled at 96°C for 2.5 hours to obtain a Chinese herbal extract.

[0065] (4) 12g of rose hydrosol and 3g of mint hydrosol were added to 42g of herbal extract to obtain a stock solution, which was then diluted 20 times. 3g of chelated platinum ion, 3g of chelated selenium ion, and 10g of bacteriostatic agent were added and stirred uniformly to obtain the herbal formula.

[0066] (Comparative Example 2) The preparation method of the herbal formula included the following steps:

[0067] (1) 10 g of bentonite was calcined at 300°C for 2 hours, cooled to room temperature, and crushed. The mixture was dispersed in 50 g of a 70 wt% aqueous ethanol solution and the pH was adjusted to 5 with hydrochloric acid. 5 g of (3-mercaptopropyl)triethoxysilane was then added, and the mixture was stirred at 45°C for 8 hours in a N2 atmosphere to react. The product was then centrifuged, washed, and dried to obtain thiolated bentonite.

[0068] (2) 3 g of (methacryloyloxyethyl)trimethylammonium chloride was added to 50 g of N,N-dimethylformamide and stirred uniformly to obtain a mixed solution. Then, 10 g of thiolated bentonite was dispersed in the mixed solution, and ultrasonic dispersion was performed for 30 minutes. Benzoin butyl ether was added, and the mixture was then dispersed at a dominant wavelength of 365 nm and an intensity of 60 mW / cm. 2 The mixture was irradiated with ultraviolet light at 45°C for 90 minutes, and the product was centrifuged, washed with isopropanol, and dried to obtain the bacteriostatic agent.

[0069] (3) 2g of safflower, 10g of peach kernel, 10g of forsythia, 11g of chrysanthemum, 10g of shiso, 22g of bellflower, 9g of star anise, 12g of wood spices, 8g of cuckoo root, 11g of soju, 11g of white atractylodes, 11g of ginger peel, 11g of clove spices, 5g of Sichuan pepper, and 16g of tea leaves were weighed and placed in a reaction vessel. 500g of deionized water was added, and the mixture was distilled at 96°C for 2.5 hours to obtain a Chinese herbal extract.

[0070] (4) 12g of rose hydrosol and 3g of mint hydrosol were added to 42g of herbal extract to obtain a stock solution, which was then diluted 20 times. 3g of chelated platinum ion, 3g of chelated selenium ion, and 10g of bacteriostatic agent were added and stirred uniformly to obtain the herbal formula.

[0071] (Comparative Example 3) The preparation method of the herbal formula included the following steps:

[0072] (1) 10 g of bentonite was calcined at 300°C for 2 hours, cooled to room temperature, and crushed. The mixture was dispersed in 50 g of a 70 wt% aqueous ethanol solution and the pH was adjusted to 5 with hydrochloric acid. 5 g of (3-mercaptopropyl)triethoxysilane was then added, and the mixture was stirred at 45°C for 8 hours in a N2 atmosphere to react. The product was then centrifuged, washed, and dried to obtain thiolated bentonite.

[0073] (2) 2g of safflower, 10g of peach kernel, 10g of forsythia, 11g of chrysanthemum, 10g of shiso, 22g of bellflower, 9g of star anise, 12g of wood spice, 8g of cuckoo root, 11g of soju, 11g of white atractylodes, 11g of rhododendron, 11g of clove spice, 5g of Sichuan pepper, and 16g of tea leaves were weighed and placed in a reaction vessel. 500g of deionized water was added, and the mixture was distilled at 96°C for 2.5 hours to obtain a Chinese herbal extract.

[0074] (3) 12g of rose hydrosol and 3g of mint hydrosol were added to 42g of herbal extract to obtain a stock solution, which was then diluted 20 times. 3g of chelated platinum ion, 3g of chelated selenium ion, and 10g of thiolated bentonite were added and the mixture was stirred uniformly to obtain the herbal formula.

[0075] Escherichia coli and Staphylococcus aureus were selected as experimental bacterial species and activated in bouillon medium at 37°C for 8 hours with a shaker speed of 150 rpm. The herbal medicine products prepared in Examples 1-3 and Comparative Examples 1-3, which had been sterilized at 121°C for 20 minutes, were then added to sterile bouillon medium at a concentration of 0.05% (w / v). Next, under sterile conditions, 1 mL of the activated bacterial solution (bacterial concentration: 109 cfu / mL) was added to 49 mL of the medium. After incubation at 37°C with a shaker speed of 150 rpm for 6 hours, the bacteria were diluted and characterized using the plate count method in accordance with the national standard GB4789.2-2022 "National Standard for Food Safety: Microbiological Testing of Foods - Colony Count Measurement." The sterilization rate was calculated using the following formula:

[0076] Bactericidal rate = (number of bacteria in the blank group - number of bacteria in the experimental group) / number of bacteria in the blank group

[0077] The unit of bacterial count was cfu / mL. See Table 1 for test results.

[0078] [Table 1]

[0079] The above description is merely a preferred specific embodiment of the present invention, and the scope of protection of the present invention is not limited thereto. Within the technical scope disclosed in the present invention, any equivalent replacement or modification made by a person skilled in the art based on the technical means and inventive idea of ​​the present invention shall be included in the scope of protection of the present invention.

Claims

1. It consists of, by weight, 8 to 12 parts rose hydrosol, 1 to 3 parts mint hydrosol, 28 to 42 parts Chinese herbal medicine extract, 1 to 3 parts chelated platinum ion, 1 to 3 parts chelated selenium ion, and 4 to 10 parts bacteriostatic agent; The method for preparing the bacteriostatic agent comprises: (1) calcining bentonite, cooling it to room temperature, pulverizing it, dispersing it in an ethanol aqueous solution, adjusting the pH with hydrochloric acid, adding (3-mercaptopropyl)triethoxysilane, stirring to react, centrifuging the product, washing and drying it to obtain thiolated bentonite; (2) adding dodecafluoroheptyl methacrylate and (methacryloyloxyethyl)trimethylammonium chloride to N,N-dimethylformamide and stirring uniformly to obtain a mixed solution; then dispersing thiolated bentonite in the mixed solution and ultrasonically dispersing; adding a photoinitiator; then irradiating with ultraviolet light to cause a reaction; centrifuging the product; washing with isopropanol; and drying to obtain a bacteriostatic agent; A Chinese herbal medicine prescription comprising:

2. 2. The herbal prescription according to claim 1, wherein in step (1), the concentration of the ethanol aqueous solution is 50-75 wt %, and the pH is adjusted to 3-5.

3. 2. The herbal prescription according to claim 1, wherein in step (1), the weight ratio of bentonite, aqueous ethanol solution, and (3-mercaptopropyl)triethoxysilane is 10:30-50:2-5.

4. In step (1), the calcination conditions are 150-300°C for 1-2 hours, and the stirring reaction conditions are N ２ The herbal medicine formulation according to claim 1, characterized in that the reaction is carried out in an atmosphere of 30 to 45°C for 5 to 8 hours.

5. 2. The herbal prescription of claim 1, wherein in step (2), the weight ratio of dodecafluoroheptyl methacrylate, (methacryloyloxyethyl)trimethylammonium chloride, N,N-dimethylformamide, and thiolated bentonite is 4-7:1-3:30-50:

10.

6. 2. The herbal formula of claim 1, wherein in step (2), the photoinitiator is one or more of benzoin dimethyl ether, benzoin ethyl ether, and benzoin butyl ether.

7. In step (2), the ultrasonic dispersion time is 15 to 30 min, the dominant wavelength of the ultraviolet light is 360 to 365 nm, and the ultraviolet light intensity is 45 to 75 mW / cm ２ The herbal medicine formulation according to claim 1, characterized in that the irradiation time is 60-90 min and the reaction temperature is 35-45°C.

8. 2. The method for preparing the herbal extract according to claim 1, further comprising the steps of: weighing 1-2 parts of safflower, 8-12 parts of peach kernel, 8-12 parts of forsythia, 8-13 parts of chrysanthemum, 7-13 parts of perilla, 18-25 parts of platycodon, 8-11 parts of star anise, 9-14 parts of sycamore, 7-12 parts of cuckoo root, 10-12 parts of atractylodes, 10-12 parts of white atractylodes, 9-13 parts of green peel, 10-12 parts of clove, 4-6 parts of Sichuan pepper, and 13-17 parts of tea leaves into a reaction vessel; adding 500 parts of deionized water; and distilling and extracting at 94-97°C for 1.5-3 hours to obtain the herbal extract.

9. A method for preparing the herbal medicine prescription according to any one of claims 1 to 8, comprising: The preparation method includes the steps of adding rose hydrosol and mint hydrosol to a Chinese herbal medicine extract to obtain a stock solution, diluting the stock solution, and then adding chelated platinum ions, chelated selenium ions and a bacteriostatic agent and stirring uniformly to obtain a Chinese herbal medicine prescription.

10. The preparation method according to claim 9, characterized in that the dilution ratio of the original solution is 10 to 20 times.

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