Composition containing plant extracts

Abstract

To provide compositions for improving blood circulation, cholesterol levels, anti-obesity, immune function, female menopause, anti-allergy, blood sugar regulation, cognitive function, liver health, and male menopause. [Solution] The present invention provides a pharmaceutical, food, or health functional food composition containing plant extracts as active ingredients, which has the effect of improving blood circulation by restoring nitric oxide (NO) reduced by oxLDL, improving cholesterol by suppressing cholesterol synthesis, having an anti-obesity effect by activating the breakdown of triglycerides, improving immune function by increasing interferon-γ, improving female menopause by activating estrogen signaling, having an anti-allergic effect by suppressing IgE that causes allergies, having a blood glucose regulating effect by increasing intracellular glucose absorption, improving cognitive function by suppressing β-secretase activity, improving liver health by restoring the viability of liver cells reduced by alcohol (ethanol) treatment, and improving male menopause by activating androgen signaling.

Description

[Technical Field]

[0001] The present invention relates to a composition containing plant extracts, which has the effects of improving blood circulation, improving cholesterol, anti-obesity, improving immune function, improving female menopause, anti-allergic effects, blood glucose regulation, improving cognitive function, improving liver health, and improving male menopause. [Background technology]

[0002] Blood clot-related diseases, which account for approximately 39% of deaths worldwide, have recently been on the rise in South Korea due to factors such as the Westernization of diets, excessive stress, and an aging population.

[0003] Currently used thrombolytic enzymes include streptokinase, urokinase, and tissue plasminogen activators (t-PA), which primarily dissolve thrombi by indirectly activating plasminogen in the body into plasmin. However, plasminogen activators have problems such as allergies, fever, local bleeding, short half-lives, and high cost, limiting their use to thrombosis treatment. Streptokinase and urokinase are exogenous enzymes that convert plasminogen into plasmin, and not only do they have side effects such as fever, allergies, local bleeding, and hypotension induction, but their high cost also means they are not used for the prevention of thrombosis in the body.

[0004] Therefore, research is needed on substances that are safe and have excellent blood circulation-improving effects.

[0005] On the one hand, cholesterol is present everywhere in our body, including the brain, nerves, muscles, skin, liver, intestines, and heart. It acts as a component of cell membranes and is known to be an important substance involved in various physiological and biochemical reactions, such as acting as a raw material for hormones, vitamin D, and bile acids involved in lipid digestion. However, on the other hand, it is known to be closely related to the onset of cardiovascular diseases, including arteriosclerosis and type 2 diabetes, which are representative adult diseases whose incidence has been rapidly increasing recently. Cholesterol is an essential substance in our bodies, but when there is an excess of cholesterol, it accumulates in vascular endothelial cells and the vascular intima, forming arteriosclerotic lesions and inducing vascular diseases such as hyperlipidemia. Hyperlipidemia, in turn, induces secondary diseases such as arteriosclerosis, hypertension, obesity, and diabetes. Thus, total blood cholesterol shows a close correlation with the development of coronary artery disease, so maintaining homeostasis of cholesterol in the blood is extremely important. Blood cholesterol levels are maintained at a certain level through synthesis in liver cells and reabsorption in intestinal cells. Hyperlipidemia refers to a condition in which excessive levels of cholesterol or triglycerides are present in the blood, inducing inflammation. While it may not present with specific symptoms, it is a risk factor for hypertension, arteriosclerosis, and stroke. When the body's proper cholesterol regulation function does not work smoothly and cholesterol accumulates, hypercholesterolemia occurs, resulting in high total cholesterol levels in the blood. When abnormalities occur in the regulation of cholesterol metabolism, not only total cholesterol but also lipoprotein components such as LDL cholesterol and HDL cholesterol change quantitatively. LDL cholesterol deposits on the walls of arteries, damaging blood vessels and inducing arteriosclerosis, while HDL cholesterol removes harmful LDL cholesterol, reducing cardiovascular diseases such as arteriosclerosis, heart disease, and stroke. Therefore, even if total cholesterol levels are normal, high levels of LDL cholesterol or low levels of HDL cholesterol can still cause arteriosclerosis.

[0006] To date, research on the effects of lowering blood cholesterol levels has focused on functional ingredients derived from natural sources that demonstrate cholesterol-improving effects, including Changnyeong onion extract, wheat β-glucan extract, flaxseed, Pu-erh tea extract, red yeast rice, plant stanol esters, policosanol-sugarcane wax alcohol, spirulina, Unibex bamboo extract, CJ hibiscus complex extract, aloe complex extract powder, and aloe extract powder. Research is ongoing to isolate substances with cholesterol-lowering effects from natural sources in order to isolate and administer substances from natural sources that can simultaneously provide sustained therapeutic and preventive effects in parallel with drug treatment for patients with high cholesterol.

[0007] On the other hand, obesity is known to be a significant risk factor for the development of diabetes, hypertension, hyperlipidemia, respiratory diseases, brain diseases, and cancer, and interest in its treatment and prevention is increasing. Currently, medical expenditures and mortality rates due to obesity and obesity-related diseases are rapidly increasing not only in South Korea but worldwide. The global obese population is currently estimated at approximately 2 billion people, and in South Korea, one in three people is classified as obese.

[0008] While various studies are being conducted to treat obesity, most obesity treatments developed to date are primarily related to mechanisms that suppress appetite or inhibit fat absorption. Obesity treatments developed through appetite-suppressing mechanisms include sibutramine, phentermine, phendimetrazine, phentermine / topiramate combinations, and lorcaserin. However, these treatments can induce central nervous system or cardiovascular side effects, and are therefore mostly prohibited or used with extreme restriction. Furthermore, orlistat, an obesity treatment drug developed in relation to a mechanism that inhibits fat absorption, is known to be relatively safe and can be taken long-term. However, side effects such as diarrhea, steatorrhea, and fecal incontinence have been reported, causing inconvenience. In particular, it has the drawback of being less effective in cases where the main cause of obesity is excessive carbohydrate intake rather than fat intake, as is the case with Koreans (Kim, Sang-man. A study on orlistat. Journal of the Korean Society for the Study of Obesity 1998;7(4):287-92). Therefore, since an obesity treatment drug with excellent weight regulation efficacy and confirmed safety for long-term use has not yet been developed, there is a need for obesity prevention or treatment drugs that utilize new mechanisms other than those already known.

[0009] On the other hand, immunostimulation is an important therapeutic strategy that reinforces the body's defense mechanisms against various diseases such as cancer and inflammatory diseases. Immunostimulation can be achieved by increasing the activity of immune cells and stimulating the immune response. For example, macrophages play a major role in the immune response. Phagocytic activity, the primary role of macrophages, involves absorbing microorganisms and other pyrogenic particles, and stimulating the immune response by secreting numerous cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-12 (IL-12), as well as cytotoxic and inflammatory substances such as nitric oxide (NO) (Wolf et al., 1994; Lee and Hong, 2011; Murray and Wynn, 2011). Therefore, increasing the activity of macrophages can be one means of achieving immunostimulation.

[0010] Natural products have been used in Asian countries to treat various diseases, particularly as tonics for immune enhancement, health maintenance, and longevity. Components of various structures, especially polysaccharides, β-glucans, saponins, and curcuminoids, have been widely presented as immune enhancers in clinical and pharmaceutical applications (Ragupathi et al., 2008; Won et al., 2011; Sun et al., 2005).

[0011] On the other hand, menopause in women is a phenomenon of menstrual cessation that occurs when the genetically predetermined function of the ovaries, which lasts for about 50 years from birth, reaches the end of its lifespan. It signifies the loss of reproductive capacity and is a physiological change, not a pathological phenomenon. Currently, the average life expectancy for Korean women is 81.2 years (2011, Statistics Korea), and if we assume that the average age of menopause for Korean women, as defined by the Korean Society of Obstetrics and Gynecology, is 50 years, it means that women live more than one-third of their lives with depleted female hormones.

[0012] As women reach menopause, the imbalance and decrease in the secretion of female hormones cause changes throughout the body, including the vascular, musculoskeletal, urogenital, and cranial nervous systems. These changes include vasomotor symptoms and psychological symptoms such as facial flushing, nocturnal sweating, sleep disturbances, fatigue, depression, anxiety, difficulty concentrating, memory impairment, painful intercourse due to atrophy of the urogenital system, frequent urination, loss of skin elasticity due to decreased collagen, sagging breasts, cardiovascular and musculoskeletal symptoms, and various diseases such as dementia. While menopausal symptoms vary from person to person, it has been reported that the more menopausal symptoms a woman experiences, the more severe and longer the symptoms are, the lower her quality of life becomes. Furthermore, menopausal symptoms are highly likely to progress into chronic diseases along with physical aging.

[0013] While hormone therapy, drug therapy, exercise therapy, and dietary therapy may be used to treat menopausal symptoms, the medically common female hormone therapy can increase the risk of breast cancer and other diseases, and long-term use can increase the incidence of uterine cancer, thrombovascular disease, gallbladder disease, and hypertension. For this reason, in recent years, much research has been conducted on phytoestrogens, which have been reported to perform functions similar to estrogen, as an alternative to estrogen therapy and other drug therapies.

[0014] On the other hand, allergy is a type of hypersensitivity reaction caused by an imbalance in the immune system. It is a reaction that appears in response to a specific antigen called an allergen, and is induced when IgE-class antibodies specific to that antigen bind to mast cells or basophils. When a large amount of allergen-specific IgE antibodies are produced, these antibodies begin to bind to Fc receptors on the surface of mast cells and basophils. When the allergen binds to these receptors, these cells are activated and various physiologically active substances (cytokines, prostaglandins, leukotrienes, histamine, bradykinin, tryptase, etc.) are secreted, inducing changes such as vasodilation, increased vascular permeability, smooth muscle contraction, and inflammatory responses. Such reactions can appear locally or systemically depending on the type and amount of mediating substances produced by these cells. Diseases include allergic atopic dermatitis, systemic allergy, allergic rhinitis, asthma, food allergy, and skin allergy. The incidence of allergic diseases increased significantly from 0.9 per 100,000 people in 1979 to 1.4 per 100,000 people in 1998, a rise of approximately 55.6%. This has led to a greater emphasis on the importance of alleviating and preventing these diseases.

[0015] Allergy treatments can be broadly classified into steroidal anti-inflammatory drugs (steroids) or non-steroidal anti-inflammatory drugs (NSAIDs), and antihistamines or anti-leukotrienes. The former have a strong immunosuppressive effect and can provide temporary sedation within a short period, but they have a range of side effects, from mild ones such as nausea to serious ones such as growth inhibition and osteoporosis, making long-term use problematic. The latter can provide temporary relief, but have side effects such as drowsiness and dizziness, limiting their use. Therefore, there is a growing need for substances derived from natural extracts that have fewer side effects in the body and high anti-allergic activity.

[0016] On the other hand, diabetes mellitus is a group of diseases characterized by chronic hyperglycemia due to insufficient insulin action, accompanied by various characteristic metabolic abnormalities. Since insulin is mainly involved in carbohydrate metabolism, the fundamental problem in diabetes is an abnormality in carbohydrate metabolism. However, this affects the metabolism of all nutrients in the body, so it can be said to be a comprehensive metabolic disease, and is considered one of the most important chronic diseases in modern times, with a particularly high incidence in developed countries. Diabetes is diagnosed based on the plasma glucose concentration in venous blood. If the plasma glucose concentration is 126 mg / dL or higher in both tests conducted on two different days while fasting (usually 8-12 hours before the test), then diabetes mellitus is diagnosed.

[0017] In South Korea, the prevalence of diabetes was 1.5% in the 1970s, but it increased to 8-10% in the 1990s, leading to a rapid increase in the incidence of chronic diabetic complications.

[0018] Diabetes is broadly classified into insulin-dependent diabetes and non-insulin-dependent diabetes. Insulin-dependent diabetes mainly develops during adolescence and always requires insulin injections, accounting for 84% of diabetes cases. Non-insulin-dependent diabetes mainly develops gradually in adults and is primarily treated with dietary therapy and exercise prescriptions.

[0019] The treatment of diabetes requires the administration of blood glucose-regulating drugs. Insulin is the most widely used conventional blood glucose-regulating drug, and sulfonylurea and biguanide drugs are the main oral formulations used.

[0020] However, these drugs are expensive, and the cost of lifelong administration is a significant burden. Furthermore, they have many side effects such as allergies, bone marrow suppression, and hypoglycemia. Therefore, caution and contraindications are necessary when using them in patients with liver or kidney impairment, hypotension, myocardial infarction, hypoxia, and the elderly.

[0021] Therefore, considering the unique aspect of this disease that it requires lifelong administration, there is a pressing need for the development of health functional foods and natural product pharmaceuticals with as few side effects as possible.

[0022] On the other hand, research has shown that cognitive impairments such as memory and learning ability loss induced by Alzheimer's disease, a type of senile dementia, begin with damage to acetylcholine neurons in the basal brain. As a result, drugs that can enhance the function of acetylcholine neurons through various mechanisms of action have been developed, including agonists targeting muscarinic acetylcholine receptors, acetylcholine production promoters, and acetylcholinesterase inhibitors. Currently, tacrine is being developed as an acetylcholinesterase inhibitor, but some side effects such as gastrointestinal disorders have occurred, so it is necessary to search for and utilize inhibitory materials from natural plant materials.

[0023] On the one hand, it has been reported that the changing drinking habits of modern people and the shift to Western eating habits that favor fatty foods are closely related to the increasing trend of fatty liver disease. Fatty liver refers to an unhealthy state where the fat content in the liver of the human body exceeds 5%. It is known to be induced by various complex causes, but broadly speaking, it can be divided into alcoholic fatty liver caused by excessive drinking and non-alcoholic fatty liver that occurs in relation to other etiologies unrelated to alcohol. Among these, alcoholic fatty liver (ASH), which is fatty liver caused by drinking, is one of the liver diseases that are the second most serious after cancer among the causes of death of adults in their 40s and 50s in developed countries. In South Korea, where alcohol intake is among the highest according to developed country standards, the incidence of fatty liver was reported to be 32% in 2009 based on the adult population. Also, different from alcoholic fatty liver, which has a direct relationship with alcohol, non-alcoholic fatty liver (NASH) means a disease in which neutral fat accumulates in the liver without relation to drinking. Clinically, it is a progressive liver disease and is recognized as a precursor disease that induces liver cirrhosis and liver cancer, and its significance is very great. In particular, major adult diseases such as obesity, insulin-resistant diabetes, and hyperlipidemia are recognized as risk factors, and active regulation and treatment are required (Jonathan et al., Metabolism. 65:1096 - 1108, 2016).

[0024] On the other hand, male menopause (PADAM; Partial Androgen Deficiency in Aging Male) is a process in which, due to the aging phenomenon of the body, men experience a decline in general physical and mental aspects. Due to the influence of the decrease in male hormones, it means that male characteristics, general vitality, and mood gradually decline. Symptoms of male menopause include nervousness, emotional instability, depression, dizziness, facial flushing, sweating, sleep disorders, decreased vitality, decreased memory, decreased work ability, decreased libido, skin aging, decreased bone density, and increased visceral fat, etc.

[0025] Just like women, men also start to experience menopause around the age of 50, and the frequency increases as they get older. As men age, the functions of the adrenal glands, sperm fertility, and Leydig cells decline, and male menopause symptoms appear while serum testosterone decreases. The cause of such male menopause, in addition to other environmental factors, is mainly due to the decline of the endocrine system centered around male hormones. Therefore, conventionally, hormone replacement therapy for men has been known as the main treatment method. Male hormone replacement therapy includes oral medications, topical preparations, patch-type preparations for sticking, injection therapy, etc.

[0026] Testosterone, a hormone related to male menopause, is the main circulating androgen in men. It is mainly secreted by the Leydig cells of the testes and is mainly involved in the development and maintenance of male secondary sexual characteristics. Testosterone circulating in the body can be metabolized by 5α-reductase into dihydrotestosterone (DHT). Testosterone can be metabolized into estradiol by the aromatase enzyme complex found in the liver, fat, and testes. When testosterone is aromatized into estradiol, male menopause symptoms such as gynecomastia symptoms can appear. Increasing testosterone and decreasing the levels of DHT and estradiol helps relieve male menopause symptoms.

[0027] The conversion of testosterone to DHT is important in many aspects. For example, DHT binds to SHBG with a much higher affinity than testosterone. Also, in many tissues, the activity of testosterone is determined by its reduction to DHT bound to the androgen receptor protein. The steroid receptor complex moves to the nucleus, and cell changes related to transcription and androgen action are initiated.

[0028] Testosterone circulates in the bloodstream bound to proteins (98%). In men, approximately 40% binds to high-affinity hormone-binding globulin (SHBG). The remaining 60% weakly binds to albumin. Therefore, a large number of testosterone levels can be measured in clinical trials. The portion of testosterone in the blood that is not bound to proteins is called "free" testosterone, and free testosterone and protein-bound testosterone together are referred to as "total testosterone" or "testosterone."

[0029] SHBG plays a role in regulating the actual effects of testosterone by binding to it. When SHBG binds to testosterone, the hormone loses its activity, and when it is separated, it becomes the hormone that actually acts. 40-80% of testosterone exists in a biologically inactive state bound to SHBG, and studies in men aged 40-70 have shown that SHBG levels increase. The increase in blood SHBG due to aging further reduces bioavailable testosterone, which can worsen symptoms of male menopause.

[0030] One treatment option for male menopausal complex syndrome is androgen replacement therapy. However, such therapy can cause hepatotoxicity and side effects such as affecting blood lipid metabolism, leading to elevated LDL and decreased HDL levels, potentially causing cardiovascular disease. Furthermore, androgen administration can have adverse effects on the liver, lipid status, cardiovascular and prostate diseases, as well as sleep and behavioral disorders, necessitating regular checkups for these side effects. On the other hand, it has been reported that androgen therapy cannot be offered in asymptomatic cases or in the presence of clear prostate cancer.

[0031] Recently, in order to overcome the problems associated with drug-based treatments, attempts have been made to develop agents for improving male menopausal complex syndrome that primarily use natural herbal medicines. However, conventional technologies using such herbal materials as main components are mainly limited to research related to sexual function, which is a fragmentary symptom of male menopausal complex syndrome, and do not fundamentally improve the various symptoms that appear due to male menopausal complex syndrome, such as osteoporosis, muscle weakness, and decreased motivation. As a result, the development of formulations for improving male menopausal complex syndrome remains insufficient. [Overview of the project] [Problems that the invention aims to solve]

[0032] Therefore, the inventors have confirmed that 111 types of plant extracts have the following effects: they restore nitric oxide (NO) reduced by oxLDL, thereby improving blood circulation; they suppress cholesterol synthesis, thereby improving cholesterol levels; they activate the breakdown of triglycerides, thereby having an anti-obesity effect; they increase interferon-γ, thereby improving immune function; they activate estrogen signaling, thereby improving symptoms of female menopause; they suppress IgE, which causes allergies, thereby having an anti-allergic effect; they increase intracellular glucose absorption, thereby regulating blood glucose levels; they suppress β-secretase activity, thereby improving cognitive function; they restore the survival rate of liver cells reduced by alcohol (ethanol) treatment, thereby improving liver health; and they activate androgen signaling, thereby improving symptoms of male menopause. Based on these findings, the inventors have completed the present invention.

[0033] Therefore, the object of the present invention is to identify the following plants: Island thistle (Cirsium nipponicum (Maxim.) Makino), Deodeok (Codonopsis lanceolata), Ulleung goldenrod (Solidago virgaurea), goat's beard (Aruncus dioicus), Ulleungdo aster (Aster glehnii), Myeongyi (Allium victorialis or Allium ulleungense), Aging chive (Allium senescens), and Silvery mountain (Sorbus commixta). ash), Thymus quinquecostatus var.Japanese knotweed (Japanese knotweed), wild chervil (Anthriscus sylvestris), giant knotweed (Fallopia sachalinensis), mono maple (Acer pictum), ulleungdo ladyfern (Athyrium acutipinnulum), wild wasabi (Wasabia japonica), yellow Mongolian snakegourd (Trichosanthes kirilowii), Polygonatum odoratum (Pungdo solomon's seal), ivy (Hedera sieboldii) Rhombea (English name: Songak), Viola woosanensis (English name: Ulleungdo sweet violet), Campanula takesimana (English name: Korean bellflower), Lilium hansonii (English name: Ulleungdo turk's-cap lily), Pseudolysimachion insulare (English name: Ulleungdo spike speedwell), Sedum oryzifolium (English name: Coastal moss-like stonecrop), Stellaria aquatica (English name: Water chickweed, Giant chickweed), Houttuynia cordata Thunb. (English name: Heartleaf) Houttuynia), Hepatica maxima (Nakai) Nakai (English name: Ulleungdo liverleaf), Ranunculus quelpaertensis (H.Lev.) Nakai), Akaso (scientific name: Boehmeria tricuspis (Hance) Makino, English name: Tricuspidate falsenettle), Ezokawaranadeshiko (scientific name: Dianthus longicalyx Miq., English name: Long-calyx pink), Nagabagishigishi (scientific name: Rumex crispus L., English name: Curled dock), Otogirisou (scientific name: Hypericum erectum Thunb., English name: Erect St. Johnswort), Amachazuru (scientific name: Gynostemma pentaphylla (Thunb.) Makino, English name: Five-leaf gynostemma), Suzumeuri (scientific name: Melothria japonica (Thunb.) Maxim., English name: Bird's egg cucumber), Takeshimahatazao (scientific name: Arabis takesimana) Nakai (English name: Ulleungdo rockcress), Kakinegarashi (scientific name: Sisymbrium officinale (L.) Scop., English name: Hedge Mustard), Hakusan Shakunage (scientific name: Rhododendron fauriei), Umegasa-sou (scientific name: Chimaphila japonica Miq., English name: Asian prince's pine), Yabukouji (scientific name: Ardisia japonica (Thunb.) Blume, English name: Marlberry), Hamabossu (scientific name: Lysimachia mauritiana Lam., English name: Spoon-leaf yellow loosestrife), Tsuruajisai (scientific name: Hydrangea petiolaris Siebold & Zucc., English name: Climbing hydrangea), Tsurumannengusa (scientific name: Sedum sarmentosum Bunge, English name: Stringy stonecrop), Takeshima-kirinsou (scientific name: Sedum takesimense Nakai, English name: Ulleungdo stonecrop), Zudayakushu (scientific name: Tiarella polyphylla D.Don, English name: Foam flower), Kawarasaiko (scientific name: Potentilla chinensis Ser.English name: East Asian cinquefoil), Indigobush (scientific name: Amorpha fruticosa L., English name: Indigobush Amorpha), Sericea lespedeza (scientific name: Lespedeza cuneata (Dum.Cours.) G.Don., English name: Sericea lespedeza), Alfalfa (scientific name: Medicago sativa L., English name: Alfalfa), Amur vetch (scientific name: Vicia amurensis Oett., English name: Amur vetch), Hairy purple loosestrife (scientific name: Lythrum salicaria L., English name: Hairy purple loosestrife), Circaea mollis Siebold & Zucc., English name: South enchanter's nightshade, Epilobium pyrricholophum Franch. & Sav. (English name: Long-seed willowherb), Evening Primrose (scientific name: Oenothera biennis L., English name: Evening Primrose), Stolon golden saxifrage (scientific name: Chrysosplenium flagelliferum F.Schmidt, English name: Stolon golden saxifrage), Serrate-petal rockfoil (scientific name: Saxifraga fortunei var. incisolobata (Engl. & Irmsch.) Nakai, English name: Serrate-petal rockfoil), Lamium takesimense NAKAI., English name: Ulleungdo deadnettle), Physocarpus insularis (Nakai) Nakai, English name: Island ninebark, Corydalis filistipes Nakai, English name: Island corydalis, Rubus takesimensis Nakai, English name: Ulleungdo raspberry), Dystaenia takeshimana (Nakai) Kitag, English name: Korea Dystaenia, Viola takeshimana Nakai, English name: Ulleungdo violet, Euonymus japonicus Thunb., English name: Spindle Tree, Vitis coignetiae Pulliat ex Planch., English name: Crimson Grapevine, Japanese angelica tree (Aralia cordata var. continentalis (Kitag.) YCChu), Japanese angelica tree (Aralia elata (Miq.) Seem.), Fatsia japonica (Thunb.) Decne. & Planch., Glossy-leaf paper plant), Three-leaf clematis (Clematis apiifolia DC.), Lyre-leaf nightshade (Solanum lyratum Thunb.), Ivy morning glory (Calystegia hederacea Wall.), Beach morning glory (Calystegia soldanella (L.) Roem. & Schult.). glory), Murasakishikibu (scientific name: Callicarpa japonica Thunb., English name: East Asian beautyberry), Kawamidori (scientific name: Agastache rugosa (Fisch. & CA)(Mey.) Kuntze (English name: Korean mint), Clinopodium chinense var. parviflorum (Kudo) H.Hara (English name: Small-flower Asian calamint), Lamium amplexicaule L. (English name: Henbit deadnettle), Leonurus japonicus Houtt. (English name: Oriental motherwort), Thalictrum kemense Fr. (English name: Long-stalk low meadow-rue), Viburnum carlesii Hemsl. (English name: Korean spice viburnum), Chelidonium majus var. asiaticum (H.Hara) Ohwi (English name: Asian greater) Celandine, Seashore Spatulate Aster (Aster spathulifolius Maxim.), Leopard Plant (Farfugium japonicum (L.) Kitam.), Oriental Yellowhead (Inula britannica var. japonica (Thunb.) Franch. & Sav.), Giant Butterbur (Petasites japonicus (Siebold & Zucc.) Maxim.), Short-stem Sedge (Carex blepharicarpa Franch.), Short-stem Sedge (Carex breviculmis R.Br.), Purple Miscanthus (Miscanthus sinensis var. purpurascens (Andersson)) Rendle (English name: Purple maiden silvergrass), Pennisetum alopecuroides (L.) Spreng.(English name: Foxtail fountaingrass), (scientific name: Pseudosas) a japonica (Siebold & Zucc.ex Steud.) Makino ex Nakai, English name: Arrow bamboo), チシマザサ (scientific name: Sasa kurilensis (Rupr.) Makino & Shibata, English name: Kuril bamboo), クララ (scientific name: Sophora flavescens Aiton, English name: Shrubby sophora), ホンカンゾウ (scientific name: Hemerocallis fulva (L.) L., English name: Orange Daylily), オニユリ (scientific name: Lilium lancifolium Thunb., English name: Tiger lily), ヤブラン (scientific name: Liriope platyphylla FTWang & T.Tang, English name: Big blue) lilyturf), ダイオウマイヅルソウ(scientific name: Maianthemum dilatatum (AWWood) A.Nelson & JFMacbr., British name: False lily of the valley), スギ(scientific name: Cryptomeria japonica (Thunb.ex Lf) Don sieboldii Carriere, English name: Ulleungdo hemlock), シロダモ (scientific name: Neolitsea sericea (Blume) Koidz., English name: Sericeous newlitsea), ユズリハ (scientific name: Daphniphyllum macropodum Miq., English name: Macropodous daphniphyllum), ムクノキ (scientific name: Aphananthe aspera (Thunb.) Planch., English name: Scabrous aphananthe), エゾエノキ (scientific name: Celtis jessoensis Koidz.)English name: Caudate-leaf hackberry), Manchurian Elm (Ulmus laciniata (Trautv.) Mayr), Montane alder (Alnus maximowiczii Callier), Ulleungdo linden (Tilia insularis Nakai), Fragrant snowbell (Styrax obassia Siebold & Zucc.), Ulleungdo flowering cherry (Prunus takesimensis Nakai), Spotted laurel (Aucuba japonica Thunb.), Ulleungdo The objective is to provide a composition for improving blood circulation, cholesterol levels, anti-obesity, immune function, female menopause, anti-allergic effects, blood sugar regulation, cognitive function, liver health, and male menopause, containing as an active ingredient one or more plant extracts selected from the group consisting of maple, Phellodendron insulare Nakai, Zanthoxylum ailanthoides Siebold & Zucc., Alianthus-like prickly-ash, Ligustrum japonicum Thunb., Wax-leaf privet, Lonicera insularis Nakai, Ulleungdo honeysuckle, and Hydrangea serrata. [Means for solving the problem]

[0034] As a means to solve the above problems, the present invention relates to Island thistle (Cirsium nipponicum (Maxim.) Makino), Deodeok (Codonopsis lanceolata), Ulleung goldenrod (Solidago virgaurea), goat's beard (Aruncus dioicus), Ulleungdo aster (Aster glehnii), Myeongyi (Allium victorialis or Allium ulleungense), Aging chive (Allium senescens), and Silvery mountain (Sorbus commixta). ash), Thymus quinquecostatus var.Japanese knotweed (Japanese knotweed), wild chervil (Anthriscus sylvestris), giant knotweed (Fallopia sachalinensis), mono maple (Acer pictum), ulleungdo ladyfern (Athyrium acutipinnulum), wild wasabi (Wasabia japonica), yellow Mongolian snakegourd (Trichosanthes kirilowii), Polygonatum odoratum (Pungdo solomon's seal), ivy (Hedera sieboldii) Rhombea (English name: Songak), Viola woosanensis (English name: Ulleungdo sweet violet), Campanula takesimana (English name: Korean bellflower), Lilium hansonii (English name: Ulleungdo turk's-cap lily), Pseudolysimachion insulare (English name: Ulleungdo spike speedwell), Sedum oryzifolium (English name: Coastal moss-like stonecrop), Stellaria aquatica (English name: Water chickweed, Giant chickweed), Houttuynia cordata Thunb. (English name: Heartleaf) Houttuynia), Hepatica maxima (Nakai) Nakai (English name: Ulleungdo liverleaf), Ranunculus quelpaertensis (H.Lev.) Nakai), Akaso (scientific name: Boehmeria tricuspis (Hance) Makino, English name: Tricuspidate falsenettle), Ezokawaranadeshiko (scientific name: Dianthus longicalyx Miq., English name: Long-calyx pink), Nagabagishigishi (scientific name: Rumex crispus L., English name: Curled dock), Otogirisou (scientific name: Hypericum erectum Thunb., English name: Erect St. Johnswort), Amachazuru (scientific name: Gynostemma pentaphylla (Thunb.) Makino, English name: Five-leaf gynostemma), Suzumeuri (scientific name: Melothria japonica (Thunb.) Maxim., English name: Bird's egg cucumber), Takeshimahatazao (scientific name: Arabis takesimana) Nakai (English name: Ulleungdo rockcress), Kakinegarashi (scientific name: Sisymbrium officinale (L.) Scop., English name: Hedge Mustard), Hakusan Shakunage (scientific name: Rhododendron fauriei), Umegasa-sou (scientific name: Chimaphila japonica Miq., English name: Asian prince's pine), Yabukouji (scientific name: Ardisia japonica (Thunb.) Blume, English name: Marlberry), Hamabossu (scientific name: Lysimachia mauritiana Lam., English name: Spoon-leaf yellow loosestrife), Tsuruajisai (scientific name: Hydrangea petiolaris Siebold & Zucc., English name: Climbing hydrangea), Tsurumannengusa (scientific name: Sedum sarmentosum Bunge, English name: Stringy stonecrop), Takeshima-kirinsou (scientific name: Sedum takesimense Nakai, English name: Ulleungdo stonecrop), Zudayakushu (scientific name: Tiarella polyphylla D.Don, English name: Foam flower), Kawarasaiko (scientific name: Potentilla chinensis Ser.English name: East Asian cinquefoil), Indigobush (scientific name: Amorpha fruticosa L., English name: Indigobush Amorpha), Sericea lespedeza (scientific name: Lespedeza cuneata (Dum.Cours.) G.Don., English name: Sericea lespedeza), Alfalfa (scientific name: Medicago sativa L., English name: Alfalfa), Amur vetch (scientific name: Vicia amurensis Oett., English name: Amur vetch), Hairy purple loosestrife (scientific name: Lythrum salicaria L., English name: Hairy purple loosestrife), Circaea mollis Siebold & Zucc., English name: South enchanter's nightshade, Epilobium pyrricholophum Franch. & Sav. (English name: Long-seed willowherb), Evening Primrose (scientific name: Oenothera biennis L., English name: Evening Primrose), Stolon golden saxifrage (scientific name: Chrysosplenium flagelliferum F.Schmidt, English name: Stolon golden saxifrage), Serrate-petal rockfoil (scientific name: Saxifraga fortunei var. incisolobata (Engl. & Irmsch.) Nakai, English name: Serrate-petal rockfoil), Lamium takesimense NAKAI., English name: Ulleungdo deadnettle), Physocarpus insularis (Nakai) Nakai, English name: Island ninebark, Corydalis filistipes Nakai, English name: Island corydalis, Rubus takesimensis Nakai, English name: Ulleungdo raspberry), Dystaenia takeshimana (Nakai) Kitag, English name: Korea Dystaenia, Viola takeshimana Nakai, English name: Ulleungdo violet, Euonymus japonicus Thunb., English name: Spindle Tree, Vitis coignetiae Pulliat ex Planch., English name: Crimson Grapevine, Japanese angelica tree (Aralia cordata var. continentalis (Kitag.) YCChu), Japanese angelica tree (Aralia elata (Miq.) Seem.), Fatsia japonica (Thunb.) Decne. & Planch., Glossy-leaf paper plant), Three-leaf clematis (Clematis apiifolia DC.), Lyre-leaf nightshade (Solanum lyratum Thunb.), Ivy morning glory (Calystegia hederacea Wall.), Beach morning glory (Calystegia soldanella (L.) Roem. & Schult.). glory), Murasakishikibu (scientific name: Callicarpa japonica Thunb., English name: East Asian beautyberry), Kawamidori (scientific name: Agastache rugosa (Fisch. & CA)(Mey.) Kuntze (English name: Korean mint), Clinopodium chinense var. parviflorum (Kudo) H.Hara (English name: Small-flower Asian calamint), Lamium amplexicaule L. (English name: Henbit deadnettle), Leonurus japonicus Houtt. (English name: Oriental motherwort), Thalictrum kemense Fr. (English name: Long-stalk low meadow-rue), Viburnum carlesii Hemsl. (English name: Korean spice viburnum), Chelidonium majus var. asiaticum (H.Hara) Ohwi (English name: Asian greater) Celandine, Seashore Spatulate Aster (Aster spathulifolius Maxim.), Leopard Plant (Farfugium japonicum (L.) Kitam.), Oriental Yellowhead (Inula britannica var. japonica (Thunb.) Franch. & Sav.), Giant Butterbur (Petasites japonicus (Siebold & Zucc.) Maxim.), Short-stem Sedge (Carex blepharicarpa Franch.), Short-stem Sedge (Carex breviculmis R.Br.), Purple Miscanthus (Miscanthus sinensis var. purpurascens (Andersson)) Rendle (English name: Purple maiden silvergrass), Chikarashiba (scientific name: Pennisetum alopecuroides (L.) Spreng., English name: Foxtail fountaingrass), Yadake (scientific name: ...).Pseudosasa japonica (Siebold & Zucc.ex Steud.) Makino ex Nakai, English name: Arrow bamboo), チシマザサ (scientific name: Sasa kurilensis (Rupr.) Makino & Shibata, English name: Kuril bamboo), クララ (scientific name: Sophora flavescens Aiton, English name: Shrubby sophora), ホンカンゾウ (scientific name: Hemerocallis fulva (L.) L., English name: Orange Daylily), オニユリ (scientific name: Lilium lancifolium Thunb., English name: Tiger lily), ヤブラン (scientific name: Liriope platyphylla FTWang & T.Tang, English name: Big blue) lilyturf), ダイオウマイヅルソウ(scientific name: Maianthemum dilatatum (AWWood) A.Nelson & JFMacbr., British name: False lily of the valley), スギ(scientific name: Cryptomeria japonica (Thunb.ex Lf) Don sieboldii Carriere, English name: Ulleungdo hemlock), シロダモ (scientific name: Neolitsea sericea (Blume) Koidz., English name: Sericeous newlitsea), ユズリハ (scientific name: Daphniphyllum macropodum Miq., English name: Macropodous daphniphyllum), ムクノキ (scientific name: Aphananthe aspera (Thunb.) Planch., English name: Scabrous aphananthe), エゾエノキ (scientific name: Celtis jessoensis Koidz.)English name: Caudate-leaf hackberry), Manchurian Elm (Ulmus laciniata (Trautv.) Mayr), Montane alder (Alnus maximowiczii Callier), Ulleungdo linden (Tilia insularis Nakai), Fragrant snowbell (Styrax obassia Siebold & Zucc.), Ulleungdo flowering cherry (Prunus takesimensis Nakai), Spotted laurel (Aucuba japonica Thunb.), Ulleungdo This invention provides a composition for improving blood circulation, cholesterol levels, anti-obesity, immune function, female menopause, anti-allergic effects, blood glucose regulation, cognitive function, liver health, and male menopause, containing one or more plant extracts selected from the group consisting of maple, Phellodendron insulare Nakai, Zanthoxylum ailanthoides Siebold & Zucc., Alianthus-like prickly-ash, Ligustrum japonicum Thunb., Wax-leaf privet, Lonicera insularis Nakai, Ulleungdo honeysuckle, and Hydrangea serrata as active ingredients. [Effects of the Invention]

[0035] The composition containing the plant extract according to the present invention has the following effects: it has a blood circulation-improving effect by restoring nitric oxide (NO) reduced by oxLDL; it has a cholesterol-improving effect by suppressing cholesterol synthesis; it has an anti-obesity effect by activating the breakdown of triglycerides; it has an immune function-improving effect by increasing interferon-γ; it has a blood glucose-regulating effect by activating estrogen signaling; it has an anti-allergic effect by suppressing IgE that causes allergies; it has a blood glucose-regulating effect by increasing intracellular glucose absorption; it has a cognitive function-improving effect by suppressing β-secretase activity; it has a liver health-improving effect by restoring the viability of liver cells reduced by alcohol (ethanol) treatment; and it exhibits a male menopausal-improving effect by activating androgen signaling. Therefore, it can be used as a pharmaceutical or food composition. [Modes for carrying out the invention]

[0036] The configuration of the present invention will be described in detail below.

[0037] This invention relates to the following plants: Island thistle (Cirsium nipponicum (Maxim.) Makino), Deodeok (Codonopsis lanceolata), Ulleung goldenrod (Solidago virgaurea), goat's beard (Aruncus dioicus), Ulleungdo aster (Aster glehnii), Myeongyi (Allium victorialis or Allium ulleungense), Aging chive (Allium senescens), and Silvery mountain (Sorbus commixta). ash), Thymus quinquecostatus var.Japanese knotweed (Japanese knotweed), wild chervil (Anthriscus sylvestris), giant knotweed (Fallopia sachalinensis), mono maple (Acer pictum), ulleungdo ladyfern (Athyrium acutipinnulum), wild wasabi (Wasabia japonica), yellow Mongolian snakegourd (Trichosanthes kirilowii), Polygonatum odoratum (Pungdo solomon's seal), ivy (Hedera sieboldii) Rhombea (English name: Songak), Viola woosanensis (English name: Ulleungdo sweet violet), Campanula takesimana (English name: Korean bellflower), Lilium hansonii (English name: Ulleungdo turk's-cap lily), Pseudolysimachion insulare (English name: Ulleungdo spike speedwell), Sedum oryzifolium (English name: Coastal moss-like stonecrop), Stellaria aquatica (English name: Water chickweed, Giant chickweed), Houttuynia cordata Thunb. (English name: Heartleaf) Houttuynia), Hepatica maxima (Nakai) Nakai (English name: Ulleungdo liverleaf), Ranunculus quelpaertensis (H.Lev.) Nakai), Akaso (scientific name: Boehmeria tricuspis (Hance) Makino, English name: Tricuspidate falsenettle), Ezokawaranadeshiko (scientific name: Dianthus longicalyx Miq., English name: Long-calyx pink), Nagabagishigishi (scientific name: Rumex crispus L., English name: Curled dock), Otogirisou (scientific name: Hypericum erectum Thunb., English name: Erect St. Johnswort), Amachazuru (scientific name: Gynostemma pentaphylla (Thunb.) Makino, English name: Five-leaf gynostemma), Suzumeuri (scientific name: Melothria japonica (Thunb.) Maxim., English name: Bird's egg cucumber), Takeshimahatazao (scientific name: Arabis takesimana) Nakai (English name: Ulleungdo rockcress), Kakinegarashi (scientific name: Sisymbrium officinale (L.) Scop., English name: Hedge Mustard), Hakusan Shakunage (scientific name: Rhododendron fauriei), Umegasa-sou (scientific name: Chimaphila japonica Miq., English name: Asian prince's pine), Yabukouji (scientific name: Ardisia japonica (Thunb.) Blume, English name: Marlberry), Hamabossu (scientific name: Lysimachia mauritiana Lam., English name: Spoon-leaf yellow loosestrife), Tsuruajisai (scientific name: Hydrangea petiolaris Siebold & Zucc., English name: Climbing hydrangea), Tsurumannengusa (scientific name: Sedum sarmentosum Bunge, English name: Stringy stonecrop), Takeshima-kirinsou (scientific name: Sedum takesimense Nakai, English name: Ulleungdo stonecrop), Zudayakushu (scientific name: Tiarella polyphylla D.Don, English name: Foam flower), Kawarasaiko (scientific name: Potentilla chinensis Ser.English name: East Asian cinquefoil), Indigobush (scientific name: Amorpha fruticosa L., English name: Indigobush Amorpha), Sericea lespedeza (scientific name: Lespedeza cuneata (Dum.Cours.) G.Don., English name: Sericea lespedeza), Alfalfa (scientific name: Medicago sativa L., English name: Alfalfa), Amur vetch (scientific name: Vicia amurensis Oett., English name: Amur vetch), Hairy purple loosestrife (scientific name: Lythrum salicaria L., English name: Hairy purple loosestrife), Circaea mollis Siebold & Zucc., English name: South enchanter's nightshade, Epilobium pyrricholophum Franch. & Sav. (English name: Long-seed willowherb), Evening Primrose (scientific name: Oenothera biennis L., English name: Evening Primrose), Stolon golden saxifrage (scientific name: Chrysosplenium flagelliferum F.Schmidt, English name: Stolon golden saxifrage), Serrate-petal rockfoil (scientific name: Saxifraga fortunei var. incisolobata (Engl. & Irmsch.) Nakai, English name: Serrate-petal rockfoil), Lamium takesimense NAKAI., English name: Ulleungdo deadnettle), Physocarpus insularis (Nakai) Nakai, English name: Island ninebark, Corydalis filistipes Nakai, English name: Island corydalis, Rubus takesimensis Nakai, English name: Ulleungdo raspberry), Dystaenia takeshimana (Nakai) Kitag, English name: Korea Dystaenia, Viola takeshimana Nakai, English name: Ulleungdo violet, Euonymus japonicus Thunb., English name: Spindle Tree, Vitis coignetiae Pulliat ex Planch., English name: Crimson Grapevine, Japanese angelica tree (Aralia cordata var. continentalis (Kitag.) YCChu), Japanese angelica tree (Aralia elata (Miq.) Seem.), Fatsia japonica (Thunb.) Decne. & Planch., Glossy-leaf paper plant), Three-leaf clematis (Clematis apiifolia DC.), Lyre-leaf nightshade (Solanum lyratum Thunb.), Ivy morning glory (Calystegia hederacea Wall.), Beach morning glory (Calystegia soldanella (L.) Roem. & Schult.). glory), Murasakishikibu (scientific name: Callicarpa japonica Thunb., English name: East Asian beautyberry), Kawamidori (scientific name: Agastache rugosa (Fisch. & CA)(Mey.) Kuntze (English name: Korean mint), Clinopodium chinense var. parviflorum (Kudo) H.Hara (English name: Small-flower Asian calamint), Lamium amplexicaule L. (English name: Henbit deadnettle), Leonurus japonicus Houtt. (English name: Oriental motherwort), Thalictrum kemense Fr. (English name: Long-stalk low meadow-rue), Viburnum carlesii Hemsl. (English name: Korean spice viburnum), Chelidonium majus var. asiaticum (H.Hara) Ohwi (English name: Asian greater) Celandine, Seashore Spatulate Aster (Aster spathulifolius Maxim.), Leopard Plant (Farfugium japonicum (L.) Kitam.), Oriental Yellowhead (Inula britannica var. japonica (Thunb.) Franch. & Sav.), Giant Butterbur (Petasites japonicus (Siebold & Zucc.) Maxim.), Short-stem Sedge (Carex blepharicarpa Franch.), Short-stem Sedge (Carex breviculmis R.Br.), Purple Miscanthus (Miscanthus sinensis var. purpurascens (Andersson)) Rendle (English name: Purple maiden silvergrass), Pennisetum alopecuroides (L.) Spreng.(English name: Foxtail fountaingrass) (Scientific name: Pseudosasa japonicus) a (Siebold & Zucc.ex Steud.) Makino ex Nakai, English name: Arrow bamboo), チシマザサ (scientific name: Sasa kurilensis (Rupr.) Makino & Shibata, English name: Kuril bamboo), クララ (scientific name: Sophora flavescens Aiton, English name: Shrubby sophora), ホンカンゾウ (scientific name: Hemerocallis fulva (L.) L., English name: Orange Daylily), オニユリ (scientific name: Lilium lancifolium Thunb., English name: Tiger lily), ヤブラン (scientific name: Liriope platyphylla FTWang & T.Tang, English name: Big blue) lilyturf), ダイオウマイヅルソウ(scientific name: Maianthemum dilatatum (AWWood) A.Nelson & JFMacbr., British name: False lily of the valley), スギ(scientific name: Cryptomeria japonica (Thunb.ex Lf) Don sieboldii Carriere, English name: Ulleungdo hemlock), シロダモ (scientific name: Neolitsea sericea (Blume) Koidz., English name: Sericeous newlitsea), ユズリハ (scientific name: Daphniphyllum macropodum Miq., English name: Macropodous daphniphyllum), ムクノキ (scientific name: Aphananthe aspera (Thunb.) Planch., English name: Scabrous aphananthe), エゾエノキ (scientific name: Celtis jessoensis Koidz., English name: Caudate-leaf hackberry), オヒョウ (scientific name: Ulmus laciniata (Trautv.)).Mayr (Manchurian Elm), Alnus maximowiczii Callier (Montane alder), Tilia insularis Nakai (Ulleungdo linden), Styrax obassia Siebold & Zucc. (Fragrant snowbell), Prunus takesimensis Nakai (Ulleungdo flowering cherry), Aucuba japonica Thunb. (Spotted laurel), Acer takesimense Nakai (Ulleungdo maple), Phellodendron insulare Nakai, Zanthoxylum (Japanese quince). This invention relates to a composition containing one or more plant extracts selected from the group consisting of Alianthus-like prickly ash (Alianthus-like prickly ash), Ligustrum japonicum Thunb. (Wax-leaf privet), Lonicera insularis Nakai (Ulleungdo honeysuckle), and Hydrangea serrata (mountain hydrangea) as an active ingredient.

[0038] A composition containing a plant extract as an active ingredient according to the present invention can be used as a composition for improving blood circulation, cholesterol levels, anti-obesity, improving immune function, improving symptoms of female menopause, anti-allergy, blood glucose regulation, improving cognitive function, improving liver health, and improving symptoms of male menopause.

[0039] As can be seen from the examples of the present invention, compositions containing the plant extract of the present invention as an active ingredient exhibit far superior effects at low concentrations on improving blood circulation, cholesterol levels, anti-obesity, immune function, female menopause, anti-allergy, blood glucose regulation, cognitive function, liver health, and male menopause. Therefore, they can be used as active ingredients in food compositions and pharmaceutical compositions for improving blood circulation, cholesterol levels, anti-obesity, immune function, female menopause, anti-allergy, blood glucose regulation, cognitive function, liver health, and male menopause.

[0040] Accordingly, the present invention also provides uses of the plant extract for the manufacture of food compositions or pharmaceutical compositions for improving blood circulation, cholesterol levels, anti-obesity, immune function, female menopause, anti-allergy, blood glucose regulation, cognitive function, liver health, and / or male menopause, and methods for improving blood circulation, cholesterol levels, anti-obesity, immune function, female menopause, anti-allergy, blood glucose regulation, cognitive function, liver health, and male menopause, comprising administering the plant extract.

[0041] In this invention, the plants used are not limited to any particular type and can be cultivated or purchased commercially, regardless of their origin.

[0042] The plant extract of the present invention may be extracted by methods known in the art, and the method is not particularly limited. Alternatively, commercially available extracts can be used.

[0043] The plant extract according to the present invention can have effects on improving blood circulation, cholesterol levels, anti-obesity, immune function, female menopause, anti-allergy, blood glucose regulation, cognitive function, liver health, and / or male menopause, regardless of region. Preferably, plant extracts collected and extracted on Ulleungdo Island can have even better effects on improving blood circulation, cholesterol levels, anti-obesity, immune function, female menopause, anti-allergy, blood glucose regulation, cognitive function, liver health, and / or male menopause.

[0044] The plant extract can be made from any part of the plant and is not limited to the part from which it is extracted. The production of the plant extract is not limited by the form of the plant, and the plant includes all parts that have undergone processing such as drying. For example, the plant may be the whole plant, roots, stems, leaves, fruits, flowers, buds, branches, bark, sap, bulbils and / or seeds of the aforementioned plant.

[0045] The term "extract" as used in this invention includes extracts obtained by extracting the aforementioned plants, diluted or concentrated extracts of the extract, dried products obtained by drying the extract, crude or refined products of the extract, fermented products of the extract, or mixtures thereof, encompassing the extract itself and all dosage forms that can be formed using the extract. It also includes juices obtained by directly pressing or crushing the plants and then filtering them. The plants can be extracted as they are or after processing. "Processing" refers to pharmaceutical techniques that alter the inherent properties of medicinal materials by processing them based on traditional Chinese medicine theory. Examples include stir-frying, roasting, which involves stir-frying the medicinal materials with a certain amount of liquid auxiliary material to allow the auxiliary material to permeate the drug tissue, and steaming, which involves adding liquid auxiliary material according to the processing regulations for each type of medicinal material, mixing, heating in a suitable container, or steaming until a certain degree is reached and then drying.

[0046] In the present invention, the extraction method is not particularly limited and can be performed by methods commonly used in the industry. Non-limiting examples of the extraction method include solvent extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc., which may be performed individually or in combination of two or more methods.

[0047] In the present invention, the type of extraction solvent used for extraction is not particularly limited, and any solvent known in the art can be used. In the present invention, the extract can be obtained by extraction with water, a lower alcohol having 1 to 6 carbon atoms, or a mixture thereof. Non-limiting examples of the extraction solvent include water; lower alcohols having 1 to 6 carbon atoms such as methanol, ethanol, propyl alcohol, and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; or mixtures thereof. Specifically, water, a lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used individually or in combination of two or more. When two or more solvents are used in combination, the mixing ratio between the solvents is not particularly limited.

[0048] In the present invention, extraction can be performed using a solvent in an amount 1 to 100 times the weight of the dried plant material, specifically 1 to 50 times, and more specifically 2 to 20 times, and at an extraction temperature of 10 to 80°C, specifically 15 to 50°C, for an extraction time of 2 hours to 30 days, specifically 12 hours to 18 days. The extraction may also include a process of performing 1 to 5 consecutive extractions containing the dried and crushed material to obtain a liquid crude extract.

[0049] In the present invention, the extract can be used as is after filtering to remove suspended solid particles, for example, by filtering the particles using nylon, filter paper, or by using cryofiltration, or after drying by freeze-drying, hot air drying, spray drying, etc.

[0050] The aforementioned liquid crude extract can be separated from the dried and crushed plant material by methods such as vacuum filtration, and then subjected to a concentration or drying process. For example, the liquid crude extract may be concentrated under reduced pressure at 20 to 100°C, preferably 30 to 70°C, using a vacuum rotary concentrator, or the liquid extract may be dried to obtain a powdered extract. The concentrated or powdered extract may be used soluble in water, alcohol, DMSO (dimethyl sulfoxide), or a mixture thereof, as needed.

[0051] In the present invention, the active ingredient may be a fraction of a plant extract.

[0052] As used in this invention, the term "fractionated product" refers to a product obtained by fractionating a mixture containing various components in order to separate a specific component or a specific group of components.

[0053] In the present invention, the fractionation method for obtaining the fraction is not particularly limited and may be carried out by methods commonly used in the industry. An example of such a fractionation method is a method in which a plant extract obtained by extracting a plant is treated with a predetermined solvent to obtain a fraction from the extract.

[0054] The type of solvent used to obtain the fraction in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fractionation solvent include polar solvents such as water and alcohol; and non-polar solvents such as hexane, ethyl acetate, chloroform, dichloromethane, and butanol. These may be used alone or in mixtures of two or more. When using an alcohol as the fractionation solvent, an alcohol having 1 to 6 carbon atoms can be used.

[0055] In this invention, "blood circulation improving effect" refers to exhibiting a blood circulation improving effect by suppressing vasodilation or platelet aggregation.

[0056] In this invention, "cholesterol-improving effect" refers to the effect of suppressing cholesterol synthesis.

[0057] In this invention, "anti-obesity effect" means being helpful in reducing body fat.

[0058] In the present invention, "immune function improving effect" means improving an immune hypersensitivity reaction or improving or enhancing immune function.

[0059] In this invention, "menopausal improvement effect" means that it is beneficial to the health of men in menopause or beneficial to the health of men in menopause.

[0060] In the present invention, "anti-allergic effect" means that the effect helps to improve an immune hypersensitivity reaction, helps to improve nasal conditions caused by an immune hypersensitivity reaction, or helps to improve skin conditions caused by an immune hypersensitivity reaction.

[0061] In this invention, "cognitive function improvement effect" refers to improving cognitive function that has declined due to aging.

[0062] In this invention, "liver health improvement effect" refers to an effect that is beneficial to liver health or protects the liver from alcohol-related damage.

[0063] In the present invention, "blood glucose regulating effect" refers to a blood glucose regulating effect that includes promoting the absorption of sugar into cells and helping to suppress the rise in blood glucose levels after a meal.

[0064] In the present invention, "effective amount" or "contained as an active ingredient" means the amount of extract that exhibits effects such as improving blood circulation, inhibiting cholesterol synthesis, exhibiting anti-obesity effects, improving immune function, improving female menopause symptoms, exhibiting anti-allergic effects, exhibiting blood glucose regulating effects, exhibiting cognitive function improving effects, exhibiting liver health improving effects, and having male menopause improving effects. When the composition of the present invention contains an effective amount of the aforementioned plant extract, it can provide desirable effects such as improving blood circulation, regulating cholesterol, anti-obesity effects, improving immune function, improving female menopause symptoms, anti-allergic effects, regulating blood glucose symptoms, improving liver health, and improving male menopause symptoms.

[0065] In the food composition and pharmaceutical composition according to the present invention, the content of the plant extract is preferably 0.0001 to 10 parts by weight relative to the total weight of the food composition and pharmaceutical composition.

[0066] The present invention provides a pharmaceutical composition containing a plant extract as an active ingredient for improving blood circulation, cholesterol levels, anti-obesity, improving immune function, improving symptoms of female menopause, anti-allergy, blood glucose regulation, improving cognitive function, improving liver health, and / or improving symptoms of male menopause.

[0067] As used in this invention, the term "pharmaceutical composition" may be used as a concept that includes the meaning of "quasi-drug" or "pharmaceutical product."

[0068] The pharmaceutical composition may be in the form of a solution, suspension, or emulsion in an oil or aqueous medium, or in the form of an extract, powder, granules, tablet, or capsule.

[0069] Furthermore, the composition may contain one or more additional active ingredients exhibiting the same or similar functions. For example, it may contain known ingredients for improving blood circulation, cholesterol, anti-obesity, improving immune function, improving female menopause, anti-allergy, blood glucose regulation, improving cognitive function, improving liver health, and improving male menopause. When additional ingredients for improving blood circulation, cholesterol, anti-obesity, improving immune function, improving female menopause, anti-allergy, blood glucose regulation, improving cognitive function, improving liver health, and improving male menopause are included, the effects of the composition of the present invention on improving blood circulation, cholesterol, anti-obesity, improving immune function, improving female menopause, anti-allergy, blood glucose regulation, improving cognitive function, improving liver health, and improving male menopause may be further enhanced. When adding such ingredients, safety of combined use, ease of formulation, and stability of the active ingredients may be considered. In one specific example of the present invention, the composition may further contain ingredients known in the industry for improving blood circulation, cholesterol, anti-obesity, improving immune function, improving female menopause, anti-allergy, blood glucose regulation, improving cognitive function, improving liver health, and improving male menopause. The additional components may be present in amounts of 0.0001 to 10 parts by weight relative to the total weight of the composition, and the range of content can be adjusted according to requirements such as safety and ease of formulation of the plant extract.

[0070] Furthermore, the composition of the present invention may further contain a pharmaceutically acceptable carrier.

[0071] Pharmaceutically acceptable carriers may contain a variety of components, such as buffer solutions, sterile water for injection, general saline or phosphate-buffered saline, sucrose, histidine, salts, and polysorbates.

[0072] The compositions of the present invention can be administered orally or parenterally, and can be administered in the form of general pharmaceutical formulations, for example, in various oral and parenteral dosage forms during clinical administration. When formulation, they can be prepared using commonly used fillers, bulking agents, binders, wetting agents, disintegrants, surfactants, and other diluents or excipients.

[0073] Solid dosage forms for oral administration include tablets, pills, powders, granules, capsules, etc. Such solid dosage forms may be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient, such as starch, calcium carbonate, sucrose or lactose, gelatin, etc.

[0074] In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid formulations for oral use include suspensions, oral solutions, emulsions, and syrups, and in addition to commonly used simple diluents such as water and liquid paraffin, they may contain various excipients, such as humectants, sweeteners, fragrances, and preservatives.

[0075] Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. Suppository bases may include witepsol, macrogol, tween 61, cocoa butter, lauric butter, and glycerogenous gelatin.

[0076] The effective amount of plant extract contained in the composition of the present invention may vary depending on the form in which the composition is commercialized, the method by which the plant extract is applied to the skin, and the duration of contact with the skin. For example, when the composition is commercialized in a pharmaceutical dosage form, it can contain the plant extract at a higher concentration than when it is commercialized as a cosmetic product for daily skin application. Therefore, the daily dose is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, based on the amount of the plant extract, and can be administered 1 to 6 times a day.

[0077] The compositions of the present invention can be used alone or in combination with methods using surgery, radiotherapy, hormone therapy, chemotherapy, and biological reaction modifiers.

[0078] Furthermore, compositions containing the plant extract of the present invention as an active ingredient for improving blood circulation, cholesterol levels, anti-obesity, improving immune function, improving symptoms of female menopause, anti-allergy, blood sugar regulation, improving cognitive function, improving liver health, and / or improving symptoms of male menopause may be provided as quasi-drugs.

[0079] In the present invention, the "quasi-drug" contains the plant extract as an active ingredient and may further contain, if necessary, a pharmaceutically acceptable carrier, excipient, or diluent. The pharmaceutically acceptable carrier, excipient, or diluent is not limited as long as it does not impair the effects of the present invention and may include, but is not limited to, fillers, bulking agents, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, or preservatives.

[0080] The quasi-drug may be, for example, a disinfectant, a shower foam, an ointment, a wet wipe, a coating agent, etc., and may preferably be manufactured as a semi-solid preparation such as an external ointment or lotion, but is not limited thereto. The formulation method, dosage, method of use, and components of the quasi-drug may be appropriately selected from common techniques known in the art.

[0081] Furthermore, the present invention provides a topical skin preparation containing the plant extract of the present invention as an active ingredient for improving blood circulation, cholesterol levels, anti-obesity, improving immune function, improving symptoms of female menopause, anti-allergy, blood glucose regulation, improving cognitive function, improving liver health, and / or improving symptoms of male menopause.

[0082] When the aforementioned plant extract is used as a topical skin preparation, it may also contain additional adjuvants commonly used in dermatology, such as fatty substances, organic solvents, solvents, concentrates, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestering agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic surfactants, lipid vesicles, or any other components commonly used in topical skin preparations. Furthermore, these components may be introduced in amounts commonly used in dermatology.

[0083] When the plant extract is provided as a dosage form for topical skin preparations, it may have dosage forms such as ointments, patches, gels, creams, or sprays, but is not limited thereto.

[0084] The present invention also relates to a food composition containing the aforementioned plant extract for improving blood circulation, cholesterol levels, anti-obesity, improving immune function, improving symptoms of female menopause, anti-allergy, blood glucose regulation, improving cognitive function, improving liver health, and improving symptoms of male menopause.

[0085] When the plant extract of the present invention is provided as a food composition, the composition may include food-grade food additives in addition to the active ingredient.

[0086] The aforementioned food additives refer to components that can be added to food as supplements, and are added to manufacture health functional foods in various dosage forms, and can be appropriately selected and used by those skilled in the art. Examples of food additives include, but are not limited to, various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, coloring agents, fillers, pectin acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, and carbonating agents used in carbonated beverages.

[0087] Furthermore, the food composition may include functional foods. In this specification, "functional foods" means a group of foods to which added value has been added using physical, biochemical, or biotechnological methods to act and express the functions of the food for a specific purpose, or foods that have been designed and processed to fully express the biological regulatory functions related to the regulation of biological defense rhythms, prevention and recovery from disease, etc., that the food composition possesses. Compared to ordinary foods, they have a more active effect on maintaining and promoting health, and health supplement foods mean foods intended for health supplementation. In some cases, the terms functional foods, health foods, and health supplement foods are used interchangeably.

[0088] Specifically, the aforementioned health functional foods are foods manufactured by adding the plant extract to food ingredients such as beverages, teas, spices, gums, and confectionery, or by encapsulating, powdering, or suspending them, and when consumed, they provide specific health benefits. However, unlike general medicines, they are made from food ingredients, and therefore have the advantage of not having the side effects that can occur with long-term use of medicines.

[0089] The food may contain food-grade food additives, and may further contain suitable carriers, excipients, and diluents commonly used in the manufacture of functional foods.

[0090] Each of the aforementioned components contained in the food composition according to the present invention may be included in the food composition according to the present invention within a range that does not exceed the maximum amount of use specified in the food safety standards of each country.

[0091] The composition may include additional components commonly used in food compositions to improve odor, taste, appearance, etc. For example, it may include vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, pantothenic acid, etc. It may also include minerals such as zinc (Zn), iron (Fe), calcium (Ca), magnesium (Mg), manganese (Mn), copper (Cu), and chromium (Cr). It may also include amino acids such as lysine, tryptophan, cysteine, and valine. There are no limitations on the form that the health functional food of the present invention may take, and it can include all foods in the ordinary sense and can be used interchangeably with terms known in the industry, such as functional foods.

[0092] Furthermore, the health functional foods of the present invention can be manufactured by mixing with other appropriate auxiliary components that may be contained in food and known additives, as selected by those skilled in the art. Examples of foods to which the additive can be added include meats, sausages, bread, chocolates, candies, snacks, confectionery, pizzas, ramen and other noodles, gums, dairy products including ice cream, various soups, beverages, teas, drinks, alcoholic beverages, and vitamin complexes. The additive can also be added to soups, teas, jellies, and juices manufactured using the plant extract of the present invention as the main component. Foods used as animal feed are also included.

[0093] When the food product is in the form of a beverage, it can contain various flavorings or natural carbohydrates as additional ingredients, as is common with beverages. The aforementioned natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As sweeteners, natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame can be used. The proportion of the natural carbohydrates is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g, per 100 mL of the composition of the present invention.

[0094] In addition to the above, the food dosage form may contain various nutrients, vitamins, electrolytes, flavorings, colorings, pectinic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, and carbonating agents used in carbonated beverages. Furthermore, the food dosage form may contain fruit pulp for the production of natural fruit juices, fruit juice beverages, and vegetable beverages. Such components can be used individually or in combination. The proportion of such additives is not particularly important, but it is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

[0095] The health functional food obtained in this manner can be consumed on a daily basis, and is therefore extremely useful as it can be expected to have significant effects such as improving blood circulation, lowering cholesterol, anti-obesity, improving immune function, improving symptoms of female menopause, anti-allergic effects, regulating blood sugar, improving cognitive function, improving liver health, and improving symptoms of male menopause.

[0096] When the aforementioned plant extract is used as a food additive, it can be added as is or used together with other foods or food ingredients, and can be used appropriately by conventional methods. The amount of active ingredients mixed can be suitably determined according to its intended use (prevention, health, or therapeutic treatment). Generally, when manufacturing food or beverages, the composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, relative to the raw materials. However, in the case of long-term intake for health and hygiene purposes, or for health regulation purposes, the amount may be less than the aforementioned range, and there are no safety issues, so the active ingredients can be used in amounts greater than the aforementioned range.

[0097] The present invention will be described in detail below with reference to examples. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following examples.

[0098] Manufacturing Example 1: Production of Plant Extracts

[0099] The whole plants, roots, stems, leaves, fruits, flowers, buds, branches, bark, sap, bulbils, or seeds of plants 1-111 (collected or purchased from various locations on Ulleungdo Island) were extracted at room temperature for 3 days in an aqueous solution of 70% ethanol equivalent to 20 times their mass, and then filtered under reduced pressure. The filtered extract was concentrated and dried using a rotary evaporator (Buchi, Switzerland) to produce plant extracts.

[0100] Experimental Example 1: Effect of improving blood circulation - Measurement of the amount of recovery from the decrease in oxLDL-induced nitric oxide

[0101] To confirm the blood circulation-improving effect of plant extracts, the amount of recovery from the reduction of nitric oxide induced by oxidized low-density lipoprotein (oxLDL) was measured. First, HUVEC cells were suspended in Ham's F-12K (Kaighn's modification) medium and dispensed into culture plates. Next, plant extracts (samples) diluted to different concentrations were dispensed into the cells. At this time, oxLDL 200 ppm was used as the positive control group. After the cells treated with the samples were cultured in a 37°C, 5% CO2 incubator for 24-48 hours, the supernatant was taken and used as the analytical sample.

[0102] To analyze nitric oxide, Griess reagent was prepared by mixing equal amounts of component A and component B. Next, 250 μl of sample (culture medium) and 50 μl of Griess reagent were placed in a plate and thoroughly mixed. Then, to prepare a sodium nitrite solution, a nitrite standard solution was diluted with distilled water to a concentration between 1 and 100 mM (reference sample). The sample and Griess reagent mixture was incubated at room temperature in the dark for 30 minutes. Subsequently, the absorbance (OD) of the nitrite-containing sample relative to the reference sample was measured at 548 nm. At this time, the positive control group was treated with 0.1 μM rosuvastatin, and the positive control group showed 100% recovery.

[0103] This experiment aimed to confirm how much nitric oxide, reduced by oxLDL, can be restored by plant extracts. The experimental results quantitatively measure the amount of nitric oxide. Since nitric oxide dilates blood vessels, a higher secretion of nitric oxide indicates a better blood circulation improvement effect.

[0104] The experimental results for each sample are shown in the table below. An increase in absorbance values ​​according to the sample concentration indicates an increase in nitric oxide (NO) production. Therefore, if the amount of nitric oxide reduced by oxLDL is restored by the sample treatment, it can be confirmed that it helps in the dilation of blood vessels in the body.

[0105] Experimental Example 2. Lipid-Cholesterol Improvement Effect - Measurement of Cholesterol Synthesis in HepG2 Cells

[0106] To confirm the lipid cholesterol-lowering effect of plant extracts, HepG2 cells were dispensed in appropriate numbers and cultured in IMEM (containing 10% FCS, 4.5 g / L glucose, 4 mM glutamine, 10 μg / ml insulin, 50 μg / ml penicillin, 50 μg / ml streptomycin, and 1% non-essential amino acids). The culture medium was changed every two days, and the cells were used in the experiment 12 days after dispensing.

[0107] Cells are given 1 ml of labeled acetate ([ 14 [C]Sodium acetate solution was added and the cells were cultured in a CO2 incubator. After 1 hour of culture, the culture medium was discarded, the cells were thoroughly washed with ice-cold PBS, 2M NaOH was added to remove the cells, and the cells were cultured at 95°C for 1 hour to allow lipid saponification to occur. Next, as a recovery marker, [ 3 [H]cholesterol (approximately 20,000 dpm / sample) was added, and lipids were extracted with chloroform-methanol 2:1 (v / v). Subsequently, lipids were separated by TLC (thin layer chromatohrapy), and the band corresponding to free cholesterol was scraped out and measured in a scintillation vial. The results for the above experiment are expressed as the amount of incorporated acetate per unit of protein (nmoles acetate incorporated / mg protein). Simvastatin and atorvastatin were used as positive controls, with IC50 values ​​of 31.8 nM and 70.4 nM, respectively.

[0108] This experiment aimed to confirm the extent to which plant extracts inhibit cholesterol synthesis, and the experimental results quantitatively measured the amount of cholesterol. A lower cholesterol level indicates a better cholesterol-lowering effect.

[0109] The experimental results for each sample are shown in the table below. The effect of the test substance on the intracellular cholesterol content is shown as a relative ratio with the control group (the group treated only with DMSO (dimethyl sulfoxide)) set to 100%, or as the amount of incorporated acetate (nmoles [ 14 The measurement was expressed as [C]-acetate incorporated / 4h / well). A higher measurement value indicates increased cholesterol synthesis, while a lower measurement indicates that it is helpful in improving cholesterol levels.

[0110] Experimental Example 3. Anti-obesity effect - Measurement of glycerol content in culture medium of 3T3-L1 fibroblasts

[0111] To confirm the anti-obesity effect of plant extracts, the glycerol content was measured in the culture medium of 3T3-L1 fibroblasts.

[0112] First, 3T3-L1 fibroblasts were cultured in DMEM medium (growth medium) containing 10% bovine serum, and then cultured in appropriate cell numbers on plates. Next, the cells were placed in DMEM (differentiation medium) plates containing 10% FBS, ensuring that there were no gaps between cells, and cultured for a further 2-3 days. The cells were then cultured for 2-3 days in a medium containing the differentiation-inducing substances insulin (5 μg / mL), dexamethasone (0.25 μM), and 3-isobutyl-1-methylxanthine (0.5 mM), after which they were cultured. Finally, the cell culture medium was changed to a medium containing only insulin (5 μg / mL), cultured for 2-3 days, and then changed to 10% FBS / DMEM for a further 6-8 days (until the cells were observed to be filled with lipids). Substances with anti-obesity effects were added before or after differentiation induction, depending on the purpose of the experiment. Cell culture medium was centrifuged at 10,000 rpm for 10 minutes, and the supernatant was obtained using a Pasteur pipette. The obtained supernatant was transferred to a plate and heated at 65°C for 8 minutes to inactivate enzymes released from the cells. Finally, the glycerol probe was dissolved in 220 μl of DMSO, and the glycerol enzyme mix (Cayman Chemical, USA; Item No. 10010962) was dissolved in assay buffer. These were then stored in the dark at -20°C until before the experiment. 10 μl of glycerol standard solution was diluted with 990 μl of assay buffer to make a 1 mM glycerol standard solution. After mixing well, 0, 2, 4, 6, 8, and 10 μl were added to each well, and then assay buffer was added to each well to bring the total volume to 50 μl. To this, 50 μl of the sample (enzyme-inactivated culture medium) was added to each well. A total of 50 μl of reaction mix was prepared, containing 46 μl of analytical buffer solution, 2 μl of glycerol probe, and 2 μl of glycerol enzyme mix per well. 50 μl of the reaction mix was added to each well containing the standard solution and sample, mixed thoroughly, and then left in the dark at room temperature for 30 minutes. After that, the absorbance was measured at 570 nm.In this study, the positive control group was treated with orlistat 0.5 ppm, and the results in the positive control group were 160% higher compared to the negative control group.

[0113] This experiment aimed to determine how much plant extracts activate the breakdown of triglycerides, and the experimental results quantified the amount of glycerol. Since glycerol is a byproduct of lipolysis, a higher glycerol level indicates a better anti-obesity effect.

[0114] The experimental results for each sample are shown in the table below. When lipolysis is enhanced by the test substance, the amount of triglycerides accumulated in adipocytes decreases, and the amount of glycerol released increases. An increase in glycerol release means that triglycerides have been broken down to that extent, confirming that the test substance has an anti-obesity effect.

[0115] Experimental Example 4. Effect on Immunity Improvement - Measurement of Interferon-γ (IFN-γ) Production in Spleen Cells / Macrophagocytes

[0116] To confirm the immune function-improving effects of plant extracts, the degree of interferon-γ production in spleen cells and macrophages was measured. First, THP-1 cells (macrophages) and NK-92 cells (natural killer cells) were suspended in RPMI (Roswell Park Memorial Institute)-1640 medium (for THP-1 cells) and MEMA (Minimum Essential Media Alpha) medium (for NK-92 cells), respectively, and dispensed into culture plates (24-well / 96-well). Plant extracts (samples) diluted to different concentrations were then dispensed into the dispensed cells. LPS (lipopolysaccharide) (0.1-1 μg / mL) was used as the positive control group. Next, the cells treated with the samples were cultured in a 37°C, 5% CO2 incubator for 24-48 hours, and the supernatant was taken and used as the analytical sample.

[0117] The experiment was analyzed using the enzyme-linked immunosorbent assay (ELISA) method. A 96-well plate was coated with IFN-γ primary antibody, and the remaining space was blocked with 1% BSA (bovine serum albumin). Subsequently, 100 μl each of distilled water (blank), IFN-γ standard solution, and sample were added and reacted at room temperature for 2 hours. 100 μl of biotin-conjugated IFN-γ secondary antibody was added and reacted at room temperature for 2 hours. 100 μl of avidin-conjugated horseradish peroxidase (HRP) was added and reacted at room temperature or 37°C for 30 minutes. 100 μl of TMB substrate solution was added and reacted at room temperature for 4–14 minutes. The reaction was terminated by adding 50 μl of stop solution (sulfuric acid). Absorbance was measured at a wavelength of 450 nm using an ELISA reader. The IFN-γ content of the sample was calculated by comparing it with the absorbance of the standard solution.

[0118] This experiment aimed to determine how much interferon-gamma levels are increased by plant extracts, and the experimental results quantitatively measure the amount of interferon-gamma. A higher interferon-gamma level indicates a better immune function improvement effect.

[0119] The experimental results for each sample are shown in the table below. Higher absorbance indicates a higher IFN-γ content. Therefore, an increase in IFN-γ production can be interpreted as having an effect on enhancing immunity, such as promoting T lymphocyte differentiation, activating macrophages, and accelerating IgG antibody production in B cells.

[0120] Experimental Example 5. Effect on improving menopausal symptoms in women - Analysis of competitive estrogen receptor ligand binding

[0121] To confirm the effect of plant extracts on improving menopausal symptoms in women, we analyzed the degree of competitive binding of ligands to estrogen receptors (ER). First, [ 310 μl of [³H]-E2 (17β-estradiol) was mixed with 50 μl of rat uterine cytosol and 230 μl of 50 mM Tris buffer (pH 7.4), and then reacted with 10 μl of plant extract diluted by concentration using a drum roller (cultured at 4 °C for 20 hours). After the reaction ended, 750 μl of cold 60% hydroxylapatite (HAP) was added, and it was placed on ice for 20 minutes while vortexing for 10 seconds at 5-minute intervals, and then centrifuged at 600 g for 5 minutes at 4 °C to separate the free ligand and the bound ligand. At this time, the free ligand means ER not bound to [³H]-E2 or the plant extract, and the bound ligand means ER bound to [³H]-E2 or the plant extract, and HAP was used to adsorb the bound ligand to separate the free ligand and the bound ligand. Next, the separated bound ligand (pellet) was washed 3 times with 2 ml of 50 mM Tris buffer, 2 ml of ethanol at 4 °C was added, and it was placed on ice for 15 minutes while vortexing for 10 seconds at 5-minute intervals, and then centrifuged at 600 g for 10 minutes at 4 °C. Through the above process, [³H]-E2 dissolves in ethanol and the rest all sinks as a pellet, extracting radiolabeled E2 ([³H]-E2). Next, the [³H]-E2 (supernatant) extracted into the supernatant was poured into a vial containing 10 ml of scintillation cocktail. Thereafter, the radioactivity contained in the vial was measured with a β-counter to determine whether the plant extract inhibits the binding of radiolabeled E2 ([³H]-E2) to ER. 3 [³H]-E2 or ER not bound to the plant extract 3 [³H]-E2 or ER bound to the plant extract 3 [³H]-E2 dissolves in ethanol and the rest all sinks as a pellet, extracting radiolabeled E2 ([³H]-E2). 3 [³H]-E2 3 [³H]-E2 (supernatant) 3The degree to which the plant extract inhibited the estrogen receptor binding affinity of E2 (Estradiol) was confirmed, thus demonstrating the plant extract's ability to bind to estrogen receptors. The results from the group in which unlabeled E2 (estradiol) at concentrations of 0.03 nM, 0.1 nM, 0.33 nM, 1 nM, 10 nM, and 100 nM were used instead of the plant extract were used as the standard curve.

[0122] This experiment aimed to determine how much plant extracts activate the signaling of estrogen, a female hormone. The results quantitatively measured the ability to bind to estrogen receptors. A better binding relationship with estrogen indicates a greater effect in improving menopausal symptoms in women.

[0123] The experimental results for each sample are shown in the table below, and the concentration of estrogen receptors (ER) was calculated from the measured radioactivity values.

[0124] Experiment Example 6. Anti-allergic effect - Measurement of IgE secretion

[0125] To confirm the anti-allergic effect of plant extracts, the amount of immunoglobulin E secreted was measured. First, U266 cells were treated with the test substance while cultured in 10% FBS RPMI1640 medium for 3 days. Three days after treatment with the test substance, the medium (cell culture medium) was collected. IgE capture antibody was placed in a plate and left for one day. The plate was blocked with 2% BSA. The collected medium (cell culture medium) was placed in the plate and shaken. IgE detection antibody was placed in the plate and shaken. Streptavidin-HRP was placed in the plate, shielded from light, and then shaken. The substrate was placed in the plate, shielded from light, and shaken until it turned blue. After confirming that the color had changed, 2N sulfuric acid solution was placed in the plate and shaken until it turned yellow. Absorbance was measured at 450 nm and 570 nm, and the result was analyzed by subtracting the absorbance at 570 nm from the absorbance at 450 nm. In this study, the positive control group was treated with 1 μM dexamethasone, which resulted in a 32% inhibitory effect, and the positive control group was treated with 0.1 μM hydrocortisone, which resulted in a 35% inhibitory effect.

[0126] This experiment aimed to determine how effectively plant extracts suppress IgE, the hormone that triggers allergies. The experimental results quantitatively measure the amount of IgE. A lower IgE level indicates a better anti-allergic effect.

[0127] The experimental results for each sample are shown in the table below. Since IgE causes immunosuppression, a decrease in IgE secretion can be seen as an improvement in allergies.

[0128] Experimental Example 7. Blood glucose regulation effect - Measurement of glucose uptake (2-NBDG method)

[0129] To confirm the blood glucose regulating effect of plant extracts, the glucose absorption capacity of cells treated with plant extracts was measured. First, C2C12 cells were cultured in 96-well plates at a rate of 1 × 10⁶ 4 After laying the substrate at a concentration of cells / well, the experiment was started when it reached a subconfluence state within 24 hours.

[0130] [Notes]

[0131] Experiments must be conducted within 48 hours of cell inoculation. If the culture time increases and the cells become too numerous, or if the cell count is excessively low, it becomes difficult to obtain accurate data.

[0132] Once saturated, the medium was removed, and each well was replaced with 100 μl of fresh medium containing or without 10 μM 2-NBDG (for the control group). At this time, the test substance to be evaluated was added and the cells were treated. After 1 hour of treatment, the wells were washed twice with pre-cold PBS, the cells from each well were collected and resuspended in 200 μl of pre-cold medium, and the nuclei were stained with 1 μg / mL PI (propidium iodine).

[0133] [Notes]

[0134] After staining the nuclei, the cells must be stored at 4°C until measurement using a fully automated cell analysis and separation system (FACS), and measurement should ideally begin within 30 minutes.

[0135] FACS measurements were taken for approximately 20 seconds, and the results of about 2,000 single-cell events were collected and statistically analyzed. A positive control group was treated with 1 mM metformin, which showed a 180% increase in activity.

[0136] This experiment aimed to determine how much plant extracts increase intracellular glucose absorption, and the experimental results quantitatively measure the amount of glucose absorbed into cells. In other words, the greater the increase in intracellular glucose absorption, the better the blood glucose regulation effect.

[0137] The experimental results for each sample are shown in the table below. Fluorescence intensity was measured, and an increase in the value indicated an increase in glucose uptake. Increased glucose uptake means a decrease in blood glucose levels, thus confirming an improvement in diabetes.

[0138] Experimental Example 8. Cognitive Function Improvement Effect - Measurement of β-Secretase Activity

[0139] To confirm the cognitive function-improving effects of plant extracts, the activity of β-secretase treated with plant extracts was measured. First, the reagents and solvents necessary for the experiment were prepared:

[0140] 1) BACE (β-secretase cleavage enzyme) analysis buffer (50 mM sodium acetate, pH 4.5) 2) BACE1 enzyme (1 unit / ml) 3) BACE1 substrate (750 nM Rh-EVNLDAEFK-quencher): 40 μl + BACE1 analysis buffer 3960 μl. Use within 24 hours of preparation. 4) Stop solution (2.5M sodium acetate)

[0141] 2. 10 μl of analysis buffer, 10 μl of BACE1 substrate, β-secretase enzyme (1 unit / ml), and 10 μl of test substance were placed in a Black 96-microwell plate.

[0142] [Notes]

[0143] The test substances were prepared and used in different concentrations. Analytical buffer was used during this process.

[0144] After measuring the fluorescence intensity at Ex 545nm / Ex 585nm, the mixture was allowed to react at room temperature for 1 hour.

[0145] [Notes]

[0146] I wrapped the plate in foil to block out the light.

[0147] The reaction was stopped by adding 10 μl of stop solution. Fluorescence intensity was measured at a wavelength of 585 nm (Ex 545 / Em). The measured fluorescence intensity was calculated using the following general formula.

[0148] [General formula] Inhibition rate (%)=[1-{(S-S0) / (C-C0)}]×100 C = Fluorescence intensity 60 minutes after treatment of the control group. C0 = Fluorescence intensity before treatment of the control group (0 minutes after treatment). S = fluorescence intensity 60 minutes after sample processing. S0 = Fluorescence intensity before sample processing (0 minutes into processing)

[0149] This experiment aimed to determine how much plant extracts inhibit β-secretase activity, and the experimental results quantitatively measure β-secretase activity. Lower β-secretase activity indicates a better cognitive function improvement effect.

[0150] The experimental results for each sample are shown in the table below. Since β-secretase cleaves the APP amino acid terminus to produce amyloid-β, which impairs cognitive function, a decrease in its activity indicates an improvement in cognitive function.

[0151] Experimental Example 9. Liver Health Improvement Effect - Measurement of Liver Cell Survival Rate

[0152] To confirm the liver health-improving effects of plant extracts, the viability of hepatocytes treated with plant extracts was measured. First, HepG2 cells were cultured in 10% FBS DMEM medium at 37°C and under 5% CO2 atmosphere conditions. Next, 3 × 10⁶ HepG2 cells were placed in a 24-well plate. 5 After sampling by cells / well, the cells were cultured for one day. After treating with the test substance at different concentrations, the cells were cultured for 24 hours.

[0153] [Notes]

[0154] When processing the test substance, the wells must be confluenced with approximately 70-80% of the cells.

[0155] The MTT solution, dissolved in PBS at a concentration of 5 mg / mL, was diluted to 10% of the culture medium. This solution was then dispensed into 24-well plates in quantities of 0.5–1.0 mL. The cells were incubated in an incubator for 4 hours.

[0156] [Notes]

[0157] Since the MTT solution is sensitive to light, the plates were wrapped in foil for incubation.

[0158] After 4 hours of incubation, the culture medium containing the MTT solution was removed, and 1 mL of DMSO was added and shaken for 10 minutes. Next, the absorbance was measured at 540 nm. At this time, the positive control group was treated with (1) 100 mM ethanol and (2) 100 mM ethanol + 100 ppm silymarin, respectively. After treatment with (1) 100 mM ethanol, the survival rate of hepatocytes decreased by 31%, and after treatment with (2) 100 mM ethanol + 100 ppm silymarin, the survival rate of hepatocytes recovered by 86%.

[0159] This experiment aimed to determine how much plant extracts could restore the survival rate of liver cells that had been reduced by alcohol (ethanol) treatment. The experimental results quantitatively measured the survival rate of liver cells. In other words, a higher survival rate of liver cells indicates a better effect on improving liver health.

[0160] The experimental results for each sample are shown in the table below. A higher absorbance value indicates a higher survival rate of liver cells, suggesting a positive effect on improving liver health.

[0161] Experimental Example 10. Effect on improving male menopause symptoms - Measurement of androgen receptor transcriptional activity

[0162] To confirm the effect of plant extracts on improving male menopausal symptoms, the transcriptional activity of the androgen receptor was measured. Specifically, experiments were performed using an LNCaP cell line into which an ARE reporter plasmid was inserted. First, the cells were cultured in 10% FBS DMEM medium at 37°C and under 5% CO2 atmosphere conditions. Then, the cells were transferred to plates and cultured for 2 days before being treated with the test substance. One day after treatment with the test substance, the substrate was added and the luciferase activity was measured.

[0163] This experiment aimed to determine how much plant extracts activate the signaling of androgens, which are male hormones. The experimental results quantitatively measure the transcriptional activity of the androgen receptor. In other words, a higher transcriptional activity of the androgen receptor indicates a better effect in improving male menopausal symptoms.

[0164] The experimental results for each sample are shown in the table below. Since androgen receptors play an important role in the function of male hormones, it can be confirmed that increasing the activity of these receptors can alleviate symptoms of male menopause.

[0165] Example 1. Southern Thistle

[0166] [Table 1]

[0167] [Table 2]

[0168] Example 2. Codonopsis lanceolata

[0169] [Table 3]

[0170] [Table 4]

[0171] Example 3. Goldenrod

[0172] [Table 5]

[0173] [Table 6]

[0174] Example 4. Aruncus dioicus

[0175] [Table 7]

[0176] [Table 8]

[0177] Example 5. Aster

[0178] [Table 9]

[0179] [Table 10]

[0180] Example 6. Wild garlic

[0181] 1) Wild garlic (Allium victorialis)

[0182] [Table 11]

[0183] [Table 12]

[0184] 2) Wild garlic (Allium ulleungense)

[0185] [Table 13]

[0186] [Table 14]

[0187] Example 7. Sekkayama leek

[0188] [Table 15]

[0189] [Table 16]

[0190] Example 8. Rowan

[0191] [Table 17]

[0192] [Table 18]

[0193] Example 9. Thyme

[0194] [Table 19]

[0195] [Table 20]

[0196] Example 10. Shaku

[0197] [Table 21]

[0198] [Table 22]

[0199] Example 11. Japanese knotweed

[0200] [Table 23]

[0201] [Table 24]

[0202] Example 12. Takeshima Iitaya

[0203] [Table 25]

[0204] [Table 26]

[0205] Example 13. *Dryopteris erythrosora*

[0206] [Table 27]

[0207] [Table 28]

[0208] Example 14. Wasabi

[0209] [Table 29]

[0210] [Table 30]

[0211] Example 15. Trichosanthes cucumeroides

[0212] [Table 31]

[0213] [Table 32]

[0214] Example 16. Toshima Amadokoro

[0215] [Table 33]

[0216] [Table 34]

[0217] Example 17. Ivy

[0218] [Table 35]

[0219] [Table 36]

[0220] Example 18. Viola japonica

[0221] [Table 37]

[0222] [Table 38]

[0223] Example 19. Campanula punctata

[0224] [Table 39]

[0225]

Table 40

[0226] Example 20. Lilium tsukushiensis

[0227]

Table 41

[0228]

Table 42

[0229] Example 21. Allomyrina dichotoma

[0230]

Table 43

Table 48

[0238] Example 24. Houttuynia cordata

[0239]

Table 49

[0240]

Table 50

[0241] Example 25. Lithospermum erythrorhizon Sieb. et Zucc

[0242]

Table 51

[0243]

Table 52

[0244] Example 26. Ardisia crenata Sims

[0245]

Table 53

[0246]

Table 54

[0247] Example 27. Lycium barbarum L

[0248]

Table 55

[0249]

Table 56

[0250] Example 28. Dianthus superbus var. japonica

[0251] [Table 57]

[0252] [Table 58]

[0253] Example 29. Long-leaved dock

[0254] [Table 59]

[0255] [Table 60]

[0256] Example 30. St. John's wort

[0257] [Table 61]

[0258] [Table 62]

[0259] Example 31. Gynostemma pentaphyllum

[0260] [Table 63]

[0261] [Table 64]

[0262] Example 32. Japanese gourd

[0263] [Table 65]

[0264] [Table 66]

[0265] Example 33. Takeshima Hatasao

[0266] [Table 67]

[0267] [Table 68]

[0268] Example 34. Kakinegarashi (Japanese mustard)

[0269] [Table 69]

[0270] [Table 70]

[0271] Example 35. Rhododendron brachycarpum

[0272] [Table 71]

[0273] [Table 72]

[0274] Example 36. Chimaphila japonica

[0275] [Table 73]

[0276] [Table 74]

[0277] Example 37. Ardisia japonica

[0278] [Table 75]

[0279] [Table 76]

[0280] Example 38. Hamaboss

[0281] [Table 77]

[0282] [Table 78]

[0283] Example 39. Climbing Hydrangea

[0284] [Table 79]

[0285] [Table 80]

[0286] Example 40. Sedum sarmentosum

[0287] [Table 81]

[0288] [Table 82]

[0289] Example 41. Solidago virgaurea

[0290] [Table 83]

[0291] [Table 84]

[0292] Example 42. Zudayakshu

[0293] [Table 85]

[0294] [Table 86]

[0295] Example 43. Bupleurum falcatum

[0296] [Table 87]

[0297] [Table 88]

[0298] Example 44. Weasel-leaved bush clover

[0299] [Table 89]

[0300] [Table 90]

[0301] Example 45. Lespedeza

[0302] [Table 91]

[0303] [Table 92]

[0304] Example 46. Medicago erythrosora

[0305] [Table 93]

[0306] [Table 94]

[0307] Example 47. Vicia cracca

[0308] [Table 95]

[0309] [Table 96]

[0310] Example 48. Lythrum salicaria

[0311] [Table 97]

[0312] [Table 98]

[0313] Example 49. *Primula japonica*

[0314] [Table 99]

[0315] [Table 100]

[0316] Example 50. Epilobium angustifolium

[0317] [Table 101]

[0318] [Table 102]

[0319] Example 51. Evening Primrose

[0320] [Table 103]

[0321] [Table 104]

[0322] Example 52. Chrysosplenium macrophyllum

[0323] [Table 105]

[0324] [Table 106]

[0325] Example 53. Daimonjisou (Saxifraga fortunei)

[0326] [Table 107]

[0327] [Table 108]

[0328] Example 54. Lamium amplexicaule

[0329] [Table 109]

[0330] [Table 110]

[0331] Example 55. Spiraea japonica

[0332] [Table 111]

[0333] [Table 112]

[0334] Example 56. Corydalis ambigua

[0335] [Table 113]

[0336] [Table 114]

[0337] Example 57. Wild Raspberry

[0338] [Table 115]

[0339] [Table 116]

[0340] Example 58. Angelica pubescens

[0341] [Table 117]

[0342] [Table 118]

[0343] Example 59. Viola tasmanthus

[0344] [Table 119]

[0345] [Table 120]

[0346] Example 60. Masaki

[0347] [Table 121]

[0348] [Table 122]

[0349] Example 61. Wild Grape

[0350] [Table 123]

[0351] [Table 124]

[0352] Example 62. Udo (Japanese spikenard)

[0353] [Table 125]

[0354] [Table 126]

[0355] Example 63. Aralia elata

[0356] [Table 127]

[0357] [Table 128]

[0358] Example 64. Fatsia japonica

[0359] [Table 129]

[0360] [Table 130]

[0361] Example 65. Clematis

[0362] [Table 131]

[0363] [Table 132]

[0364] Example 66. Japanese honeysuckle

[0365] [Table 133]

[0366] [Table 134]

[0367] Example 67. Cordyceps sinensis

[0368] [Table 135]

[0369] [Table 136]

[0370] Example 68. Beach morning glory

[0371] [Table 137]

[0372] [Table 138]

[0373] Example 69. Japanese beautyberry (Callicarpa japonica)

[0374] [Table 139]

[0375] [Table 140]

[0376] Example 70. Kawamidori

[0377] [Table 141]

[0378] [Table 142]

[0379] Example 71. *Clinopodium gracile*

[0380] [Table 143]

[0381] [Table 144]

[0382] Example 72. Lamium amplexicaule

[0383] [Table 145]

[0384] [Table 146]

[0385] Example 73. Leonurus japonicus

[0386] [Table 147]

[0387] [Table 148]

[0388] Example 74. Japanese Thalictrum (Larch)

[0389] [Table 149]

[0390] [Table 150]

[0391] Example 75. Viburnum dilatatum

[0392] [Table 151]

[0393] [Table 152]

[0394] Example 76. Celandine

[0395] [Table 153]

[0396] [Table 154]

[0397] Example 77. Chrysanthemum

[0398] [Table 155]

[0399] [Table 156]

[0400] Example 78. Farfugium japonicum

[0401] [Table 157]

[0402] [Table 158]

[0403] Example 79. Oguruma

[0404] [Table 159]

[0405] [Table 160]

[0406] Example 80. Butterbur

[0407] [Table 161]

[0408] [Table 162]

[0409] Example 81. Carex takeshimaensis

[0410] [Table 163]

[0411] [Table 164]

[0412] Example 82. Carex dispalata

[0413] [Table 165]

[0414] [Table 166]

[0415] Example 83. Purple Miscanthus

[0416] [Table 167]

[0417] [Table 168]

[0418] Example 84. Pennisetum alopecuroides

[0419] [Table 169]

[0420] [Table 170]

[0421] Example 85. Yadake

[0422] [Table 171]

[0423] [Table 172]

[0424] Example 86. Sasa kurilensis

[0425] [Table 173]

[0426] [Table 174]

[0427] Example 87. Clara

[0428] [Table 175]

[0429] [Table 176]

[0430] Example 88. Hemerocallis fulva

[0431] [Table 177]

[0432] [Table 178]

[0433] Example 89. Tiger Lily

[0434] [Table 179]

[0435] [Table 180]

[0436] Example 90. Liriope muscari

[0437] [Table 181]

[0438] [Table 182]

[0439] Example 91. Daioh Mai-tsuru-sou (Daioh Mai-tsuru-sou)

[0440] [Table 183]

[0441] [Table 184]

[0442] Example 92. Japanese cedar

[0443] [Table 185]

[0444] [Table 186]

[0445] Example 93. Machilus thunbergii

[0446] [Table 187]

[0447] [Table 188]

[0448] Example 94. Japanese White Pine

[0449] [Table 189]

[0450] [Table 190]

[0451] Example 95. Tsuga

[0452] [Table 191]

[0453] [Table 192]

[0454] Example 96. *Neolitsea sericea*

[0455] [Table 193]

[0456] [Table 194]

[0457] Example 97. Daphniphyllum macropodum

[0458] [Table 195]

[0459] [Table 196]

[0460] Example 98. Aphananthe aspera

[0461] [Table 197]

[0462] [Table 198]

[0463] Example 99. Japanese hackberry (Celtis sinensis)

[0464] [Table 199]

[0465] [Table 200]

[0466] Example 100. Halibut

[0467] [Table 201]

[0468] [Table 202]

[0469] Example 101. Alnus japonica

[0470] [Table 203]

[0471] [Table 204]

[0472] Example 102. *Lindens serrata*

[0473] [Table 205]

[0474] [Table 206]

[0475] Example 103. Styrax obassia

[0476] [Table 207]

[0477] [Table 208]

[0478] Example 104. Takeshima cherry

[0479] [Table 209]

[0480] [Table 210]

[0481] Example 105. Aoki

[0482] [Table 211]

[0483] [Table 212]

[0484] Example 106. *Okinohauchiwa*

[0485] [Table 213]

[0486] [Table 214]

[0487] Example 107. Striped Amur Cork Tree

[0488] [Table 215]

[0489] [Table 216]

[0490] Example 108. Japanese Prickly Porphyra

[0491] [Table 217]

[0492] [Table 218]

[0493] Example 109. Ligustrum japonicum

[0494] [Table 219]

[0495] [Table 220]

[0496] Example 110. Golden Lobe

[0497] [Table 221]

[0498] [Table 222]

[0499] Example 111. Mountain Hydrangea

[0500] [Table 223]

[0501] [Table 224]

[0502] Formulation Example 1: Manufacturing of Pharmaceutical Preparations

[0503] 1. Manufacturing of powdered medicines

[0504] Plant extract 0.001g 1g lactose

[0505] The aforementioned ingredients were mixed and filled into airtight pouches to produce a powder.

[0506] 2. Manufacturing of tablets

[0507] Plant extract 0.2mg Corn starch 100mg Lactose 100mg Magnesium stearate 2mg

[0508] After mixing the aforementioned components, tablets were manufactured by compressing them using a standard tablet manufacturing method.

[0509] 3. Manufacturing of capsules

[0510] Plant extract 0.2mg 100mg of corn starch Lactose 100mg Magnesium stearate 2mg

[0511] After mixing the aforementioned ingredients, the mixture was filled into gelatin capsules using a standard capsule manufacturing method to produce capsules.

[0512] 4. Manufacturing of pills

[0513] Plant extract 0.003g Lactose 1.5g 1g glycerin 0.5g xylitol

[0514] After mixing the aforementioned ingredients, the product was manufactured using a standard method to produce 4g portions.

[0515] 5. Manufacturing of granules

[0516] 2mg plant extract Soybean extract 50mg Glucose 200mg Starch 600mg

[0517] After mixing the aforementioned ingredients, 100 mg of 30% ethanol was added and dried at 60°C to form granules, which were then filled into packets.

[0518] Formulation example 2: Food manufacturing

[0519] A food product containing the plant extract of the present invention was manufactured as follows.

[0520] 1. Manufacturing of wheat flour products

[0521] 0.05 to 1.0 parts by weight of the aforementioned plant extract was added to wheat flour, and this mixture was used to produce bread, cakes, cookies, crackers, and noodles, thereby manufacturing health foods.

[0522] 2. Manufacturing of dairy products

[0523] 0.2 parts by weight of the aforementioned plant extract was added to milk, and various dairy products such as butter and ice cream were produced using the milk.

[0524] 3. Manufacturing of Zen food

[0525] Brown rice, barley, glutinous rice, and Job's tears were gelatinized using a known method, dried, roasted, and then ground into a 60-mesh powder using a pulverizer. Black beans, black sesame seeds, and perilla seeds were also steamed and dried using a known method, roasted, and then ground into a 60-mesh powder using a pulverizer. The plant extracts were concentrated under reduced pressure using a vacuum concentrator, dried using a spray and hot air dryer, and the resulting dried product was ground into a 60-mesh powder using a pulverizer.

[0526] The dried powders of grains, nuts, and plant extracts produced as described above were blended with 100 parts by weight of the mixed powder in the following proportions.

[0527] Grains (30 parts by weight of brown rice, 15 parts by weight of barley, 20 parts by weight of barley), Seeds and nuts (perilla seeds 7 parts by weight, black beans 8 parts by weight, black sesame seeds 7 parts by weight), Plant extract (0.1 part by weight), Reishi mushroom (0.5 parts by weight), Zi-O (0.5 weight units)

[0528] Formulation example 3: Beverage manufacturing

[0529] 1. Manufacturing of health drinks

[0530] Plant extract 0.1mg 1000mg of citric acid 100g of oligosaccharides Plum concentrate 2g Taurine 1g Add purified water to make a total of 900 mL

[0531] After mixing the above-mentioned ingredients using a conventional method for manufacturing health beverages, the mixture was stirred and heated at 85°C for approximately one hour. The resulting solution was then filtered, placed in a sterilized 2L container, sealed and sterilized, and then stored in a refrigerator before being used in the production of the health beverage composition of the present invention.

[0532] The aforementioned composition ratio is a preferred example of a mixture of ingredients relatively suitable for beverages, but the blending ratio may be arbitrarily modified according to regional and ethnic preferences such as target market, country of origin, and intended use.

[0533] 2. Manufacturing of vegetable juice

[0534] A vegetable juice for promoting health was prepared by adding 1 g of the plant extract of the present invention to 1,000 mL of tomato or carrot juice.

[0535] 3. Manufacturing of fruit juice

[0536] A health-promoting fruit juice was produced by adding 1g of plant extract to 1,000mL of apple or grape juice.

Claims

1. Southern Thistle (scientific name: Cirsium nipponicum (Maxim.) Makino, English name: Island thistle), Codonopsis lanceolata (scientific name: Deodeok), Solidago virgaurea (scientific name: Solidago virgaurea, English name: Ulleung goldenrod), Aruncus dioicus (scientific name: Aruncus dioicus, English name: goat's bear), Aster glehnii (scientific name: Ulleungdo aster), Wild Garlic (scientific name: Allium victorialis or Allium) Myeongyi, Allium senescens, Silver mountain ash, Thymus quinquecostatus var. japonica HARA, Wild chervil, Fallopia sachalinensis, Giant knotweed, Acer palmatum pictum (English name: Mono maple), Takeshima ferns (scientific name: Athyrium acutipinnulum, English name: Ulleungdo ladyfern), Wasabi (scientific name: Wasabia japonica, English name: Wild wasabi), Yellow Mongolian snakegourd (scientific name: Trichosanthes kirilowii, English name: Yellow Mongolian snakegourd), Teshima amadokoro (scientific name: Polygonatum odoratum, English name: Pungdo solomon's seal), Hedera Rhombea (English name: Songak), Viola woosanensis (English name: Ulleungdo sweet violet), Campanula takeshimana (English name: Korean bellflower), Lilium takeshima*Hansonia hansonii* (English name: Ulleungdo turk's-cap lily), *Pseudolysimachion insulare* (English name: Ulleungdo spike speedwell), *Sedum oryzifolium* (English name: Coastal moss-like stonecrop), *Stellaria aquatica* (English name: Water chickweed, Giant chickweed), *Houttuynia cordata Thunb.* (English name: Heartleaf) Houttuynia), Hepatica maxima (Nakai) Nakai, English name: Ulleungdo liverleaf, Ranunculus quelpaertensis (H. Lev.) Nakai, Boehmeria tricuspis (Hance) Makino, English name: Tricuspidate false nettle, Dianthus longicalyx Miq., English name: Long-calyx pink, Rumex rotundifolia Crispus L. (English name: Curled dock), Hypericum erectum Thunb. (English name: Erect St. Johnsworth), Gynostemma pentaphylla (Thunb.) Makino (English name: Five-leaf gynostemma), Melothria japonica (Thunb.) Maxim. (English name: Bird's egg cucumber), Arabis takeshimana (English name: Arabis takeshimana) Nakai (English name: Ulleungdo rockcresses), Kakinegarashi (scientific name: Sisymbrium officinale (L.) Scop., English name: Hedge Mustard), Hakusan Shakunage (scientific name: Rhododendron fauriei), Umegasa-sou (scientific name: Chimaphila japonica Miq., English name: Asian prince's)Pine), Ardisia japonica (Thunb.) Blue (English name: Marlberry), Lysimachia mauritiana Lam. (English name: Spoon-leaf yellow loosestrif), Hydrangea petiolaris Siebold & Zucc. (English name: Climbing hydrangea), Sedum sarmentosum Bunge (English name: Stringy stonecrop), Sedum sarmentosum (Scientific name: Sedum sarmentosum) Takesimense Nakai (English name: Ulleungdo stonecrop), Zudayakushu (scientific name: Tiarella polyphylla D. Don, English name: Foam flower), Kawarasaiko (scientific name: Potentilla chinensis Ser., English name: East Asian cinquefoil), Itachihagi (scientific name: Amorpha fruticosa L., English name: Indigobush Amorpha), Medohagi (scientific name: Lespedeza cuneata (Dum. Cours.) G. Don., English name: Sericea) Lespedeza), Medicago sativa L., Alfalfa, Vicia amurensis Oett., Amur vetch, Lythrum salicaria L., Hairy purple loosestrife, Circaea mollis Siebold & Zucc., South enchanter's nightshade, Epilobium pyrricholophum Franch. & Sav. (English name: Long-seeded willowherb), Evening Primrose (scientific name: Oenothera biennis L., English name: Evening Primrose), Stron golden (scientific name: Chrysosplenium flagelliferum F. Schmidt, English name: Stron golden)Saxifraga, Saxifraga fortunei var. incisolobata (Engl. & Irmsch.) Nakai, English name: Serrate-petal rockfoil, Lamium takesimense Nakai, Ulleungdo deadnettle, Physocarpus insularis (Nakai) Nakai, English name: Island ninebark, Corydalis filistipes Nakai (English name: Island corydalis), Rubus takesimensis Nakai (English name: Ulleungdo raspberry), Ulleungdo raspberry (Scientific name: Dystaenia) takeshimana (Nakai) Kitag, English name: Korea Dystaenia), Viola takeshimana Nakai (scientific name: Viola takeshimana Nakai, English name: Ulleungdo violet), Masaki (scientific name: Euonymus) japonicus Thunb., English name: Spindle Tree), Yamabudu (scientific name: Vitis coignetiae Pulliat) ex Planch. , English name: Crimson grapevine), Udo (scientific name: Aralia cordata var. continentalis (Kitag.) Y.C. Chu), Aralia elata (Scientific name: Aralia elata (Miq.) Seem., English name: Japanese Angelica), Glossy-leaf paper (Scientific name: Fatsia japonica (Thunb.) Decne. & Planch., English name: Glossy-leaf paper plant), Button crane (scientific name: Clematis apiifolia DC., English name: Three-leaf) Clematis), Solanum lyratum Thunb. (English name: Lyre-leaf nightshade), Calystegia hederacea Wall. (English name: Ivy morning glory), Calystegia soldanella (Scientific name: Calystegia soldanella)(L.) R. em. & Schult. , English name: Beach morning glory, Callicarpa japonica Thunb. (scientific name: East Asian beautyberry), Agastache rugosa (Fisch. & C.A. Mey.) Kuntze (scientific name: Korean mint), Clinopodium chinense var. parviflora (Kudo) H. Hara (scientific name: Small-flower Asian calamint), Lamium amplexicaule (scientific name: Lamium amplexicaule) L. (English name: Henbit deadnettle), Leonurus japonicus Houtt. (English name: Oriental motherworth), Thalictorum kemense Fr. (English name: Long-stalk low meadow-rue), Viburnum carlesii Hemsl. (English name: Korean spice viburnum), Chelidonium majus var. asiaticum (H. Hara) Ohwi (English name: Asian greater) Celandine, Daruma chrysanthemum (scientific name: Aster spathulophilus Maxim., English name: Seashore spatulate aster), Tsuwabuki (scientific name: Farfugium japonicum (L.) Kita) m. English name: Leopard plant), Oguruma (scientific name: Inula brittannica var. japonica (Thunb.) Franch. & Sav., English name: Oriental yellowhead), Fuki (scientific name: Petasites japonicus (Siebold & Zucc.) Maxim., English name: Giant butterbur), Takeshima sedge (scientific name: Carex blepharicarpa Franch.), Aosuge (scientific name: Carex breviculmis R.Br., English name: Short-stem sedge), Murasaki sugisuki (scientific name: Miscanthus) *Sinensis var. purpurascens (Anderson) Rendle, English name: Purple maiden silvergrass*, Pennisetum alopecuroides (L.) Spreng., English name: Foxtail fountaingrass*, *Pseudosasa japonica (Si)* ebold & Zucc. ex Steud. Makino ex Nakai (English name: Architect bamboo), Chishimazasa (scientific name: Sasa kurilensis (Rupr.) Makino & Shibata (English name: Kuril bamboo), Kurara (scientific name: Sophora flavescens Aiton (English name: Shrubby sophora), Honkanzō (scientific name: Hemerocallis fulva (L.) L. (English name: Orange) Daylily), オニユリ (scientific name: Lilium lancifolium Thumb., British name: Tiger lily), ヤブラン (scientific name: Liriope Platyphylla F. T. Wang & T. Tang, English name: Big blue lilyturf), ダイオウマイヅルソウ (scientific name: Maianthemum dilatatum (A.W.Wood) A. Nelson & J. F. Macbr. , English name: False lily of the valley), Sugi (scientific name: *Cryptomeria japonica* (Thunb. ex L. f.) D. Don, English name: Japanese Cedar), Tabunok (scientific name: *Machilus thunbergii* Siebold & Zucc., English name: Thunberg's Bay-Tree), Himekomats (scientific name: *Pinus paravivora* Siebold & Zucc., English name: Ullengdo white) pine), ツガ (scientific name: Tsuga sieboldi Carriere, British name: Ulleungdo hemlock), シロダモ (scientific name: Neolitsea sericea (Blume) Koidz. , English name: Sericeous newlitsea), ユズリハ (scientific name: Daphniphyllum macropodum Miq., English name: Macropodous daphniphyllum), ムクノキ (scientific name: Aphananthe aspera (Thunb.) Plant. (English name: Scabrous aphananthe), エゾエノキ (scientific name: Celtis jessonensis Koidz., English name: Caudate-leaf hackberry), オヒョウ (scientific name: Ulmus laciniata (Trautv.))Mayr (English name: Manchurian Elm), Alnus maximowiczii Callier (English name: Montane alder), Tilia insularis Nakai (English name: Ulleungdo linden), Styrax obassia Siebold & Zucc. (English name: Fragrant snowbell), Prunus takesimensis Nakai (English name: Ulleungdo flowering cherry), Aucuba japonica Thunb. (English name: Spotted laureth), Okinohauchiwa (scientific name: Acer takesimense Nakai, English name: Ulleungdo maple), Shimakihada (scientific name: Phellodendron insulare Nakai), Karasuzansho (scientific name: Zanthoxylum alianthoides Siebold & Zucc., English name: Alianthus-like prickly-ash), Nezumimori (scientific name: Ligustrum japonicum Thunb., English name: Wax-leaf private), Kinginboku (scientific name: Lonicera insularis) A food composition for improving blood circulation, cholesterol levels, anti-obesity, improving immune function, improving symptoms of female menopause, anti-allergic effects, blood sugar regulation, cognitive function, liver health, or male menopause, containing one or more plant extracts selected from the group consisting of Nakai (English name: Ulleungdo honeysuckle) and Hydrangea serrata (scientific name: Hydrangea serrata) as an active ingredient.

2. The food composition according to claim 1, wherein the plant extract is an extract of water, an organic solvent, or a mixture thereof from a plant.

3. The food composition according to claim 1, wherein the organic solvent is a lower alcohol having 1 to 6 carbon atoms, a polyhydric alcohol, a hydrocarbon solvent, or a mixture thereof.

4. The food composition according to claim 1, wherein the plant extract is contained in an amount of 0.0001 to 10 parts by weight per 100 parts by weight of the composition.

5. The food composition according to claim 1, wherein the composition is a health functional food.

6. Southern Thistle (scientific name: Cirsium nipponicum (Maxim.) Makino, English name: Island thistle), Codonopsis lanceolata (scientific name: Deodeok), Solidago virgaurea (scientific name: Solidago virgaurea, English name: Ulleung goldenrod), Aruncus dioicus (scientific name: Aruncus dioicus, English name: goat's bear), Aster glehnii (scientific name: Ulleungdo aster), Wild Garlic (scientific name: Allium victorialis or Allium) Myeongyi, Allium senescens, Silver mountain ash, Thymus quinquecostatus var. japonica HARA, Wild chervil, Fallopia sachalinensis, Giant knotweed, Acer palmatum pictum (English name: Mono maple), Takeshima ferns (scientific name: Athyrium acutipinnulum, English name: Ulleungdo ladyfern), Wasabi (scientific name: Wasabia japonica, English name: Wild wasabi), Yellow Mongolian snakegourd (scientific name: Trichosanthes kirilowii, English name: Yellow Mongolian snakegourd), Teshima amadokoro (scientific name: Polygonatum odoratum, English name: Pungdo solomon's seal), Hedera Rhombea (English name: Songak), Viola woosanensis (English name: Ulleungdo sweet violet), Campanula takeshimana (English name: Korean bellflower), Lilium takeshima*Hansonia hansonii* (English name: Ulleungdo turk's-cap lily), *Pseudolysimachion insulare* (English name: Ulleungdo spike speedwell), *Sedum oryzifolium* (English name: Coastal moss-like stonecrop), *Stellaria aquatica* (English name: Water chickweed, Giant chickweed), *Houttuynia cordata Thunb.* (English name: Heartleaf) Houttuynia), Hepatica maxima (Nakai) Nakai, English name: Ulleungdo liverleaf, Ranunculus quelpaertensis (H. Lev.) Nakai, Boehmeria tricuspis (Hance) Makino, English name: Tricuspidate false nettle, Dianthus longicalyx Miq., English name: Long-calyx pink, Rumex rotundifolia Crispus L. (English name: Curled dock), Hypericum erectum Thunb. (English name: Erect St. Johnsworth), Gynostemma pentaphylla (Thunb.) Makino (English name: Five-leaf gynostemma), Melothria japonica (Thunb.) Maxim. (English name: Bird's egg cucumber), Arabis takeshimana (English name: Arabis takeshimana) Nakai (English name: Ulleungdo rockcresses), Kakinegarashi (scientific name: Sisymbrium officinale (L.) Scop., English name: Hedge Mustard), Hakusan Shakunage (scientific name: Rhododendron fauriei), Umegasa-sou (scientific name: Chimaphila japonica Miq., English name: Asian prince's)Pine), Ardisia japonica (Thunb.) Blue (English name: Marlberry), Lysimachia mauritiana Lam. (English name: Spoon-leaf yellow loosestrif), Hydrangea petiolaris Siebold & Zucc. (English name: Climbing hydrangea), Sedum sarmentosum Bunge (English name: Stringy stonecrop), Sedum sarmentosum (Scientific name: Sedum sarmentosum) Takesimense Nakai (English name: Ulleungdo stonecrop), Zudayakushu (scientific name: Tiarella polyphylla D. Don, English name: Foam flower), Kawarasaiko (scientific name: Potentilla chinensis Ser., English name: East Asian cinquefoil), Itachihagi (scientific name: Amorpha fruticosa L., English name: Indigobush Amorpha), Medohagi (scientific name: Lespedeza cuneata (Dum. Cours.) G. Don., English name: Sericea) Lespedeza), Medicago sativa L., Alfalfa, Vicia amurensis Oett., Amur vetch, Lythrum salicaria L., Hairy purple loosestrife, Circaea mollis Siebold & Zucc., South enchanter's nightshade, Epilobium pyrricholophum Franch. & Sav. (English name: Long-seeded willowherb), Evening Primrose (scientific name: Oenothera biennis L., English name: Evening Primrose), Stron golden (scientific name: Chrysosplenium flagelliferum F. Schmidt, English name: Stron golden)Saxifraga, Saxifraga fortunei var. incisolobata (Engl. & Irmsch.) Nakai, English name: Serrate-petal rockfoil, Lamium takesimense Nakai, Ulleungdo deadnettle, Physocarpus insularis (Nakai) Nakai, English name: Island ninebark, Corydalis filistipes Nakai (English name: Island corydalis), Rubus takesimensis Nakai (English name: Ulleungdo raspberry), Ulleungdo raspberry (Scientific name: Dystaenia) takeshimana (Nakai) Kitag, English name: Korea Dystaenia), Viola takeshimana Nakai (scientific name: Viola takeshimana Nakai, English name: Ulleungdo violet), Masaki (scientific name: Euonymus) japonicus Thunb., English name: Spindle Tree), Yamabudu (scientific name: Vitis coignetiae Pulliat) ex Planch. , English name: Crimson grapevine), Udo (scientific name: Aralia cordata var. continentalis (Kitag.) Y.C. Chu), Aralia elata (Scientific name: Aralia elata (Miq.) Seem., English name: Japanese Angelica), Glossy-leaf paper (Scientific name: Fatsia japonica (Thunb.) Decne. & Planch., English name: Glossy-leaf paper plant), Button crane (scientific name: Clematis apiifolia DC., English name: Three-leaf) Clematis), Solanum lyratum Thunb. (English name: Lyre-leaf nightshade), Calystegia hederacea Wall. (English name: Ivy morning glory), Calystegia soldanella (Scientific name: Calystegia soldanella)(L.) R. em. & Schult. , English name: Beach morning glory, Callicarpa japonica Thunb. (scientific name: East Asian beautyberry), Agastache rugosa (Fisch. & C.A. Mey.) Kuntze (scientific name: Korean mint), Clinopodium chinense var. parviflora (Kudo) H. Hara (scientific name: Small-flower Asian calamint), Lamium amplexicaule (scientific name: Lamium amplexicaule) L. (English name: Henbit deadnettle), Leonurus japonicus Houtt. (English name: Oriental motherworth), Thalictorum kemense Fr. (English name: Long-stalk low meadow-rue), Viburnum carlesii Hemsl. (English name: Korean spice viburnum), Chelidonium majus var. asiaticum (H. Hara) Ohwi (English name: Asian greater) Celandine, Daruma chrysanthemum (scientific name: Aster spathulophilus Maxim., English name: Seashore spatulate aster), Tsuwabuki (scientific name: Farfugium japonicum (L.) Kita) m. English name: Leopard plant), Oguruma (scientific name: Inula brittannica var. japonica (Thunb.) Franch. & Sav., English name: Oriental yellowhead), Fuki (scientific name: Petasites japonicus (Siebold & Zucc.) Maxim., English name: Giant butterbur), Takeshima sedge (scientific name: Carex blepharicarpa Franch.), Aosuge (scientific name: Carex breviculmis R.Br., English name: Short-stem sedge), Murasaki sugisuki (scientific name: Miscanthus) *Sinensis var. purpurascens (Anderson) Rendle, English name: Purple maiden silvergrass*, Pennisetum alopecuroides (L.) Spreng., English name: Foxtail fountaingrass*, *Pseudosasa japonica (Si)* ebold & Zucc. ex Steud. Makino ex Nakai (English name: Architect bamboo), Chishimazasa (scientific name: Sasa kurilensis (Rupr.) Makino & Shibata (English name: Kuril bamboo), Kurara (scientific name: Sophora flavescens Aiton (English name: Shrubby sophora), Honkanzō (scientific name: Hemerocallis fulva (L.) L. (English name: Orange) Daylily), オニユリ (scientific name: Lilium lancifolium Thumb., British name: Tiger lily), ヤブラン (scientific name: Liriope Platyphylla F. T. Wang & T. Tang, English name: Big blue lilyturf), ダイオウマイヅルソウ (scientific name: Maianthemum dilatatum (A.W.Wood) A. Nelson & J. F. Macbr. , English name: False lily of the valley), Sugi (scientific name: *Cryptomeria japonica* (Thunb. ex L. f.) D. Don, English name: Japanese Cedar), Tabunok (scientific name: *Machilus thunbergii* Siebold & Zucc., English name: Thunberg's Bay-Tree), Himekomats (scientific name: *Pinus paravivora* Siebold & Zucc., English name: Ullengdo white) pine), ツガ (scientific name: Tsuga sieboldi Carriere, British name: Ulleungdo hemlock), シロダモ (scientific name: Neolitsea sericea (Blume) Koidz. , English name: Sericeous newlitsea), ユズリハ (scientific name: Daphniphyllum macropodum Miq., English name: Macropodous daphniphyllum), ムクノキ (scientific name: Aphananthe aspera (Thunb.) Plant. (English name: Scabrous aphananthe), エゾエノキ (scientific name: Celtis jessonensis Koidz., English name: Caudate-leaf hackberry), オヒョウ (scientific name: Ulmus laciniata (Trautv.))Mayr (English name: Manchurian Elm), Alnus maximowiczii Callier (English name: Montane alder), Tilia insularis Nakai (English name: Ulleungdo linden), Styrax obassia Siebold & Zucc. (English name: Fragrant snowbell), Prunus takesimensis Nakai (English name: Ulleungdo flowering cherry), Aucuba japonica Thunb. (English name: Spotted laureth), Okinohauchiwa (scientific name: Acer takesimense Nakai, English name: Ulleungdo maple), Shimakihada (scientific name: Phellodendron insulare Nakai), Karasuzansho (scientific name: Zanthoxylum alianthoides Siebold & Zucc., English name: Alianthus-like prickly-ash), Nezumimori (scientific name: Ligustrum japonicum Thunb., English name: Wax-leaf private), Kinginboku (scientific name: Lonicera insularis) A pharmaceutical composition for improving blood circulation, cholesterol levels, anti-obesity, improving immune function, improving female menopausal symptoms, anti-allergic effects, blood sugar regulation, improving cognitive function, improving liver health, or improving male menopausal symptoms, comprising one or more plant extracts selected from the group consisting of Nakai (English name: Ulleungdo honeysuckle) and Hydrangea serrata (scientific name: Hydrangea serrata) as an active ingredient.

7. The pharmaceutical composition according to claim 6, wherein the plant extract is an extract of water, an organic solvent, or a mixture thereof from a plant.

8. The pharmaceutical composition according to claim 6, wherein the organic solvent is a lower alcohol having 1 to 6 carbon atoms, a polyhydric alcohol, a hydrocarbon solvent, or a mixture thereof.

9. The pharmaceutical composition according to claim 6, wherein the plant extract is contained in an amount of 0.0001 to 10 parts by weight per 100 parts by weight of the composition.

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