Platelet counting method and platelet counting system

Abstract

This invention provides a platelet counting method that enables highly sensitive and stable platelet counting even after a significant amount of time has passed since blood collection. [Solution] A platelet counting method comprising bringing a collected blood sample 20 into contact with a substrate 12 having a platelet-solid phase portion 14 on its surface, and washing the substrate 12 to which the blood sample 20 has adhered while the platelets 26 contained in the blood sample 20 are solidified on the substrate 12.

Claims

1. A blood sample is brought into contact with a substrate having a platelet-solid phase on its surface. With the platelets contained in the blood sample immobilized on the substrate, the substrate to which the blood sample is attached is washed. Platelet counting method.

2. Remove a portion of the blood sample that has come into contact with the substrate. The platelet counting method according to claim 1.

3. The platelets immobilized on the substrate are imaged. The platelet counting method according to claim 1 or 2.

4. From the captured image containing the platelets, the platelets and platelet aggregates are detected. The platelet counting method according to claim 3.

5. From the captured image including the platelets, red blood cells and white blood cells contained in the blood sample are detected. The detected red blood cells and white blood cells are excluded, and the platelets and platelet aggregates are detected. The platelet counting method according to claim 4.

6. The activation state of the platelets is determined based on the ratio of the number of platelet aggregates to the number of platelets and platelet aggregates detected. The platelet counting method according to claim 4.

7. Based on the size of the detected platelet aggregates, the activation state of the platelets is determined. The platelet counting method according to claim 4.

8. The platelet solid phase is collagen, The collagen is immobilized on the surface of the substrate. The platelet counting method according to claim 1 or 2.

9. A platelet immobilization apparatus for washing a substrate to which platelets contained in a blood sample collected from a blood sample are immobilized on a substrate having a platelet-solid phase on its surface, and the substrate to which the blood sample is attached is in a state where platelets contained in the blood sample are immobilized on the substrate. An image analysis device that detects platelets and platelet aggregates from an image containing the captured platelets, A platelet counting system having the following features.

10. The aforementioned image analysis device is From the captured image including the platelets, red blood cells and white blood cells contained in the blood sample are detected. The detected red blood cells and white blood cells are excluded, and the platelets and platelet aggregates are detected. The platelet counting system according to claim 9.

11. The image analysis device determines the activation state of the platelets based on the ratio of the number of platelet aggregates to the number of detected platelets and platelet aggregates. The platelet counting system according to claim 9 or 10.

12. The image analysis device determines the activation state of the platelets based on the size of the detected platelet aggregates. The platelet counting system according to claim 9 or 10.

Images