Saffron polypeptide fermentation micrograin structure

Abstract

This invention provides a saffron polypeptide fermentation microparticle structure that significantly improves the antioxidant capacity (AOC) of human retinal pigment epithelial cells, reduces the expression of inflammatory cytokines (TNF-α), and maintains the health and stability of retinal cells. [Solution] The saffron polypeptide fermentation microparticle structure 100 comprises an inner core layer 10, a coating layer 30, a protective layer 40, and a plurality of active particles 20. The inner core layer contains saffron polypeptide fermentate and a substrate material that forms microparticles, thereby forming a microparticle core. The plurality of active particles are dispersed inside and / or on the surface of the inner core layer. The coating layer covers the periphery of the inner core layer, and the protective layer is placed outside the coating layer and has a three-dimensional network protective structure made of biocompatible material. This improves the structural stability of the inner core layer and the active particles.

Description

Technical Field

[0001] The present invention relates to a microparticle structure of bioactive substances, and in particular, relates to a saffron polypeptide fermented microparticle structure that comprises an inner core layer, a coating layer, a protective layer and active particles, and has a multilayer and multi-point type active carrier structure.

Background Art

[0002] Macular degeneration, diabetic retinopathy and various retinal degenerative diseases are currently the leading causes of visual impairment. Studies have shown that the pathological mechanisms of these diseases are closely related to the imbalance of oxidative stress and sustained inflammatory response in retinal pigment epithelial cells, and the abnormal accumulation of free radicals induces the release of inflammatory mediators such as tumor necrosis factor (TNF-α), thereby causing cell death and retinal tissue damage.

[0003] Various active components extracted from saffron, or polypeptide fermentates obtained by microbial fermentation technology, have been applied in the health field as commercial products, but these bioactive components still have technical bottlenecks in practical applications.

[0004] First, the structural stability is insufficient. The active polypeptide chains and trace molecules contained in the saffron polypeptide fermentate are susceptible to the influences of physical shearing during the manufacturing process, temperature and humidity fluctuations in the storage environment, and the physiological environment inside the ocular tissue (for example, pH value changes and enzymatic action), which results in the collapse of the spatial structure or the deactivation of active sites.

[0005] Second, the bioavailability is limited. Since conventional extracts or simple mixing formulations lack a stable carrier system or physical barrier, it is difficult for the active components to effectively break through the physiological barrier of the retina. In addition, due to the lack of a slow-release mechanism, the concentration of the active component at the ocular lesion site decreases rapidly, making it difficult to maintain long-term antioxidant and anti-inflammatory effects.

[0006] Thirdly, there is a lack of precise overlap protection technology. Conventional techniques often result in simple mixed encapsulation, making it difficult to achieve spatial restriction and stable containment of specific active ingredients through the design of multilayer structures.

[0007] Therefore, a key challenge that this invention aims to solve is to develop a microparticle structure with a physical and geometric combination that stably encapsulates the activity of saffron polypeptide fermentate through multilayer coating technology, possessing both antioxidant effects and highly efficient anti-inflammatory capabilities, thereby overcoming the shortcomings of conventional technologies. [Overview of the project] [Problems that the invention aims to solve]

[0008] This invention proposes a saffron polypeptide fermentation microparticle structure, and aims to enable the formation of stable microparticles in saffron polypeptide fermented products through a specific spatial arrangement, multilayer coating, and three-dimensional network protective structure. This significantly improves the antioxidant capacity (AOC) of ophthalmic cells, reduces the expression of inflammatory cytokines (TNF-α), and helps maintain the health and stability of retinal cells. [Means for solving the problem]

[0009] The saffron polypeptide fermentation microparticle structure according to this invention comprises an inner core layer, a coating layer, a protective layer, and a plurality of active particles. The inner core layer contains a saffron polypeptide fermentate and a substrate material that forms microparticles, thereby forming a microparticle core. The plurality of active particles are dispersed inside and / or on the surface of the inner core layer. The coating layer covers the periphery of the inner core layer. The protective layer is installed outside the coating layer and has a three-dimensional network protective structure made of a biocompatible material, thereby improving the structural stability of the inner core layer and the active particles. [Effects of the Invention]

[0010] The inner core layer, coating layer, and protective layer all form a multilayer microparticle structure, and the active particles are uniformly distributed to form a multipoint active carrier structure, thereby significantly improving the antioxidant capacity (AOC) of human retinal pigment epithelial cells, reducing the expression of inflammatory cytokines (TNF-α), and maintaining the health and stability of retinal cells. [Brief explanation of the drawing]

[0011] [Figure 1] Figure 1 is a three-dimensional cross-sectional view showing the internal layering of the saffron polypeptide fermentation microparticle structure of the present invention. [Figure 2] Figure 2 is a schematic diagram showing the structural arrangement of the saffron polypeptide fermentation microparticle structure of the present invention. [Figure 3] Figure 3 is a bar graph showing the effect of this invention on the antioxidant capacity (AOC) of human retinal pigment epithelial cells. [Figure 4] Figure 4 is a bar graph showing the effect of this invention on the expression level of tumor necrosis factor-α (TNF-α), an indicator of inflammation in human retinal pigment epithelial cells. Forms for implementing a utility model

[0012] We will now further describe the present invention in conjunction with drawings of preferred embodiments of the present invention so that those skilled in the art may carry out the present invention in accordance with the description herein.

[0013] As shown in Figure 1-2, the saffron polypeptide fermentation microparticle structure 100 according to the present invention comprises an inner core layer 10, a plurality of active particles 20, a coating layer 30, and a protective layer 40.

[0014] The inner core layer 10 functions as the inner core entity of the microparticle structure 100 and contains saffron polypeptide fermentate and a substrate material (e.g., a biocompatible polymer material) that forms microparticles, thereby forming the microparticle core.

[0015] The active particles 20 are dispersed within and / or on the surface of the inner core layer 10 (dispersed internally or scattered on the outer surface). In specific embodiments, the active particles 20 may be uniformly distributed within and on the outer surface of the inner core layer 10, thereby forming a multi-point active carrier structure. The active particles 20 may be biocompatible natural polymers, mineral powders, or sustained-release functional materials, and these materials function not only as physical reinforcements of the fine-particle structure 100 but also as active carriers. The dispersed arrangement of the active particles 20 significantly increases the structural strength of the inner core layer 10, extending the release length of the saffron polypeptide active component and improving adsorption efficiency.

[0016] The coating layer 30 surrounds the inner core layer 10 and functions as a first barrier to protect the active ingredients.

[0017] The protective layer 40 is installed on the outside of the coating layer 30 and has a three-dimensional network protective structure made of a biocompatible material (for example, a biocompatible polysaccharide or lipid monolayer / bilayer material), thereby improving the structural stability of the inner core layer 10 and the active particles 20.

[0018] As described above, when the inner core layer 10, coating layer 30, and protective layer 40 all form a multilayer microparticle structure, the protective layer 40 and coating layer 30 perform spatial barrier and positional restriction functions through the design of multilayer coating and geometric barriers. This restricts the spatial movement of the inner core layer 10 and active particles 20, preventing component degradation and thus confine the biological activity of the saffron polypeptide.

[0019] To verify the practical biological activity and functional effects of the saffron polypeptide fermentation microparticle structure 100 of this invention, a functional test will next be performed on human retinal pigment epithelial cells.

[0020] Test of antioxidant capacity (AOC): Reference is made to the bar graph 50 showing the effect on antioxidant capacity (AOC) in Figure 3. In this test, cells were divided into a control group, an induction group subjected to oxidation induction, a positive control group administered with β-carotene, and experimental groups respectively administered with 200 μg / mL and 400 μg / mL of saffron polypeptide fermented microparticles 100. According to the experimental results, compared with the induction group, the cellular antioxidant capacity (AOC) of the experimental groups respectively administered with 200 μg / mL and 400 μg / mL of saffron polypeptide fermented microparticles 100 was both significantly improved, and among them, the improvement range of the experimental group administered with 400 μg / mL of fermented microparticles reached 19.7%. This result verifies that the structure of the present invention effectively enhances the cellular antioxidant defense mechanism and alleviates retinal damage caused by oxidative stress.

[0021] Test for reducing the expression level of inflammatory indicator (TNF-α): Reference is made to the bar graph 60 showing the effect on the expression level of inflammatory indicator (TNF-α) in Figure 4. In this test, cells were divided into a control group, an induction group subjected to inflammation induction, a positive control group administered with β-carotene, and experimental groups respectively administered with 200 μg / mL and 400 μg / mL of saffron polypeptide fermented microparticles 100. According to the experimental results, compared with the induction group, the TNF-α expression levels of the experimental groups respectively administered with 200 μg / mL and 400 μg / mL of saffron polypeptide fermented microparticles 100 are both significantly decreased. According to statistics, the inflammatory indicator (TNF-α) of human retinal pigment epithelial cells can be reduced by 26.9% in the experimental group administered with 400 μg / mL of saffron polypeptide fermented microparticles.

[0022] In summary, after the saffron polypeptide fermented microparticle structure 100 of the present invention is administered to retinal pigment epithelial cells, it is confirmed that synergistic biological activity occurs in the spatial arrangement of the structure, and the following specific practical effects are provided.

[0023] 1. Stabilize the spatial structure to efficiently encapsulate activity: The protective layer 40 with a multilayer structure and a three-dimensional network protective structure effectively limits the spatial displacement of the inner core layer 10 and the active particles 20, and greatly improves the physical stability and activity retention rate of the microparticle structure.

[0024] 2. Significantly enhance the antioxidant capacity of cells: Under an oxidative stress environment, the antioxidant capacity (AOC) of human retinal pigment epithelial cells is increased by up to 19.7%, enabling harmful free radicals to be effectively removed.

[0025] 3. Highly efficiently inhibit the ocular inflammatory response: In an inflammatory state, the inflammatory indicator (TNF-α) is reduced by 26.9%, enabling the effect of protecting the health and stability of the retina to be effectively achieved. [Description of Symbols]

[0026] 100 - Saffron polypeptide fermented microparticle structure; 10 - Inner core layer; 20 - Active particle; 30 - Coating layer; 40 - Protective layer; 50 - Bar graph showing the effect on antioxidant capacity (AOC); 60 - Bar graph showing the effect on the expression level of inflammatory indicator (TNF-α)

Claims

1. It comprises an inner core layer, a coating layer, a protective layer, and multiple active particles, The inner core layer contains a saffron polypeptide fermentation product and a substrate material that forms fine particles, thereby forming a fine particle core. Multiple of the active particles are dispersed within and / or on the surface of the inner core layer, The coating layer covers the periphery of the inner core layer, The protective layer is installed on the outside of the coating layer and has a three-dimensional mesh protective structure made of a biocompatible material, thereby improving the structural stability of the inner core layer and the active particles. A saffron polypeptide fermentation microparticle structure characterized by the above.

2. The saffron polypeptide fermentation microparticle structure according to claim 1, characterized in that the inner core layer, the coating layer, and the protective layer all form a multilayer microparticle structure.

3. The saffron polypeptide fermentation microparticle structure according to claim 1, characterized in that the active particles are uniformly distributed inside and on the outer surface of the inner core layer, thereby forming a multi-point active carrier structure.

Images