Methods for producing allogeneic CAR T cells

Abstract

Described herein are improved media for culturing immune cells and methods for using them. In particular, the cell growth media described herein are particularly suitable for T cell expansion and can be used to produce cells useful for adoptive cell therapy, including therapy using chimeric antigen receptors (e.g., CAR-T cell therapy).

Description

[Technical field]

[0001] cross reference This application claims the benefit of priority to U.S. Provisional Patent Application No. 62 / 839,449, filed April 26, 2019, the contents of which are incorporated herein by reference in their entirety.

[0002] The present disclosure relates to cell growth media and methods for the production of engineered immune cells, including chimeric antigen receptors (CARs) and engineered T cell receptors (TCRs), and methods of using same to treat cancer in patients.

[0003] Sequence Listing This application contains a Sequence Listing that has been submitted electronically in ASCII format and is incorporated herein by reference in its entirety. The ASCII copy, created on April 17, 2020, is named AT-025_02WO_ST25.txt and is 15,405 bytes in size. [Background technology]

[0004] Adoptive transfer of immune cells genetically modified to recognize malignant tumor-associated antigens has shown promise as a new approach to treat cancer (see, e.g., Brenner et al., Current Opinion in Immunology, 22(2):251-257(2010); Rosenberg et al., Nature Reviews Cancer, 8(4):299-308(2008)). Immune cells can be genetically modified to express chimeric antigen receptors (CARs) (see, e.g., Eshhar et al., Proc. Natl. Acad. Sci. USA, 90(2):720-724(1993); and Sadelain et al., Curr. Opin. Immunol, 21(2):215-223(2009)). CAR-containing immune cells, e.g., CAR-T cells (CAR-T), are engineered to have antigen specificity while retaining or enhancing their ability to recognize and kill target cells. Effective immune cell growth media and methods of use thereof may be particularly useful for the production of engineered immune cells, such as CAR-T. Provided herein are cell growth media and methods of production that address this need. Summary of the Invention

[0005] Described herein is an improved medium for culturing immune cells, and a method of use.For example, described herein is a medium that is particularly suitable for T cell expansion, which can be used to produce cells useful for adoptive cell therapy, including therapy using chimeric antigen receptors (e.g., CAR-T cell therapy).

[0006] In one aspect, the disclosure provides a cell growth medium for T cell proliferation comprising a first stimulant and a second stimulant for cell proliferation, each independently selected from the group consisting of IL-4, IL-7, IL-10, IL-12, and IL-15, wherein the first stimulant and the second stimulant are present in a concentration ratio of about 1000:1 to about 4:1.

[0007] In one aspect, the disclosure provides a cell growth medium for T cell proliferation comprising an extracellular regulator of cellular metabolism, the extracellular potassium being at a concentration of about 4 mM to about 40 mM.

[0008] In one aspect, the disclosure provides a cell growth medium for T cell proliferation comprising a first stimulant of cell proliferation and a second stimulant, each independently selected from the group consisting of IL-4, IL-7, IL-10, IL-12, and IL-15, and an extracellular regulator of cell metabolism that is extracellular potassium at a concentration of about 4 mM to about 40 mM, wherein the first stimulant and the second stimulant are present in a concentration ratio of about 1000:1 to about 4:1.

[0009] In some embodiments, the first stimulator of cell proliferation is IL7.

[0010] In some embodiments, the second stimulator of cell proliferation is IL15.

[0011] In some embodiments, the first stimulant and the second stimulant are present in a concentration ratio of about 500:1 to about 10:1, about 250:1 to about 10:1, about 200:1 to about 10:1, about 150:1 to about 10:1, about 100:1 to about 10:1, about 500:1 to about 50:1, about 250:1 to about 50:1, about 200:1 to about 50:1, about 150:1 to about 50:1, about 100:1 to about 50:1, about 500:1 to about 75:1, about 250:1 to about 75:1, about 200:1 to about 75:1, about 150:1 to about 75:1, about 100:1 to about 75:1, about 10:1 to 4:1, about 8:1 to 4:1, or about 7:1 to about 6:1.

[0012] In some embodiments, the first stimulating agent and the second stimulating agent are present in a concentration ratio of about 150:1, about 140:1, about 130:1, about 120:1, about 110:1, about 100:1, about 90:1, about 80:1, or about 70:1.

[0013] In some embodiments, the first stimulating agent is IL-7 present at a concentration of about 100 IU / mL to about 5,000 IU / mL, and the second stimulating agent is IL-15 present at a concentration of about 1 IU / mL to about 100 IU / mL.

[0014] In some embodiments, IL-7 is present at a concentration of about 300 IU / mL to about 5000 IU / mL and IL-15 is present at a concentration of about 25 IU / mL to about 50 IU / mL.

[0015] In some embodiments, IL-7 is present at a concentration of about 5000 IU / mL and IL-15 is present at a concentration of about 50 IU / mL.

[0016] In some embodiments, the first stimulatory agent and the second stimulatory agent are added to the cell culture simultaneously or sequentially.

[0017] In some embodiments, extracellular potassium is present at a concentration of about 4 mM to about 40 mM, about 10 mM to about 35 mM, about 10 mM to about 25 mM, about 20 mM to about 35 mM, about 20 mM to about 25 mM, or about 20 mM to about 30 mM.

[0018] In some embodiments, extracellular potassium is present at a concentration of about 20 mM or about 25 mM.

[0019] In some embodiments, extracellular potassium is present at a concentration of less than 40 mM and greater than 4 mM, less than 40 mM and greater than 10 mM, less than 40 mM and greater than 20 mM, or less than 40 mM and greater than 25 mM.

[0020] In some embodiments, the extracellular potassium is present as KCl.

[0021] In some embodiments, the obtained population of T cells is CM Cells and / or T SCM It is enriched in cells.

[0022] In another aspect, the disclosure provides a method of obtaining a population of T cells in vitro comprising culturing an initial population of T cells in a cell growth medium comprising a first stimulant of cell growth and a second stimulant of cell growth, each independently selected from the group consisting of IL-4, IL-7, IL-10, IL-12, and IL-15, and an extracellular regulator of cell metabolism, the extracellular potassium being at a concentration of about 4 mM to about 40 mM, the first stimulant and the second stimulant being present in a concentration ratio of about 1000:1 to about 4:1, and the obtained population of T cells is a T cell. CM Cells and / or T SCM It is enriched in cells.

[0023] In some embodiments, the cell growth medium is a cell growth medium described above.

[0024] In some embodiments, the obtained T cell population is at least about 30%, 35%, 40%, or 45% T SCM Contains cells.

[0025] In some embodiments, the obtained T cell population comprises at least about 40% T SCM Contains cells.

[0026] In some embodiments, the obtained population of T cells is about 100-fold to about 1000-fold greater than the initial population of T cells when measured over a period of about 7-16 days.

[0027] In some embodiments, the obtained population of T cells is about 100-fold to about 1000-fold greater than the initial population of T cells when measured over a period of about 10-14 days.

[0028] In some embodiments, the obtained population of T cells is at least about 100- to about 200-fold greater than the initial population of T cells.

[0029] In some embodiments, the obtained T cell population is a T cell population that is greater than or equal to the initial population of T cells when measured over a period of about 7-16 days. CM and / or T SCM From T CMand / or T SCM is enriched by about 100 to 1,000 times.

[0030] In some embodiments, the obtained T cell population is a T cell population that is greater than or equal to the initial population of T cells when measured over a period of about 10-14 days. CM and / or T SCM From T CM and / or T SCM is enriched by about 100 to 1,000 times.

[0031] In some embodiments, the obtained T cell population is a T cell population of the initial population of measured T cells. CM and / or T SCM From T CM and / or T SCM is enriched at least about 100-fold or at least about 200-fold.

[0032] In some embodiments, the initial population of T cells is an engineered T cell population.

[0033] In some embodiments, the initial population of T cells is a T cell population that expresses one or more chimeric antigen receptors.

[0034] In some embodiments, the T cells are allogeneic T cells.

[0035] In some embodiments, the T cells are autologous T cells.

[0036] In one aspect, the present disclosure provides a population of engineered immune cells, the population comprising T cells expressing one or more chimeric antigen receptors (CAR T cells), said CAR T cells being obtained using the cell expansion medium described above.

[0037] In one aspect, the present disclosure provides a population of engineered immune cells, said population comprising T cells expressing one or more chimeric antigen receptors (CAR T cells), said (CAR T cells) being obtained using the methods described above.

[0038] In some embodiments, the CAR T cells are at least about 30%, 35%, 40%, or 45% T SCM Contains cells.

[0039] In some embodiments, the CAR T cells are at least about 40% T SCM Contains cells.

[0040] In one aspect, the present disclosure provides a pharmaceutical composition comprising the population of engineered immune cells described above.

[0041] In one aspect, the present disclosure provides a method of treating a disease or disorder in a subject in need thereof comprising administering to the subject the engineered immune cells described above, or the pharmaceutical composition described above.

[0042] In some embodiments, the disease or disorder is cancer.

[0043] In one aspect, the present disclosure provides an article of manufacture comprising the engineered immune cells described above, or the pharmaceutical composition described above.

[0044] All embodiments presented herein apply to all aspects disclosed throughout this disclosure. [Brief description of the drawings]

[0045] [Figure 1] Figure 1A shows a comparison of CAR T+ phenotypes using processes including continuous IL-2 stimulation, IL-2 stimulation followed by IL-7+IL-15 stimulation, and continuous IL-7+IL-15 stimulation. The IL-7+IL-15 based process increases the amount of TSCM CAR T cells.

[0046] FIG. 1B shows the cytokine release capacity of CD19-specific CAR T cells upon target cell exposure, where CAR T cells were prepared using a manufacturing process including IL-2 stimulation, IL-2 stimulation followed by IL-7+IL-15 stimulation, and sequential IL-7+IL-15 stimulation.

[0047] [Diagram 2] Figure 2A shows the effect of increased extracellular potassium (40 mM, unfilled bars) to increase the amount of TSCM cells that both process IL-2 and IL-7+15, compared to normal (4 mM) potassium concentrations (filled bars).

[0048] Figure 2B shows the effect of increased extracellular potassium (40 mM, open bars) on the proliferative capacity of allogeneic CAR T cells in both IL-2 and IL-7+15 processes compared to normal potassium concentrations (4 mM, filled bars).

[0049] [Diagram 3] Figure 3 shows the effect of extracellular potassium concentrations of 25 mM and 10 mM, which did not negatively affect the proliferation of human T cells during allogeneic CAR T cell manufacturing compared to higher concentrations.

[0050] [Figure 4] FIG. 4 shows that maximum preservation of TSCM cells was achieved at 25 mM extracellular potassium.

[0051] [Diagram 5] FIG. 5 shows that improved potency of Flt3-specific CAR T cells (Allo-819) was obtained using a combination of IL-7+15-based processes with 25 mM extracellular potassium supplementation. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0052] Described herein are cell growth media and methods that are particularly useful for culturing immune cells.

[0053] Adoptive cell therapy using engineered immune cells is a type of immunotherapy that has emerged as a particularly promising new approach for cancer treatment. The production and manufacture of engineered immune cells involves the collection of immune cells (e.g., T cells) from a subject, followed by in vitro cell expansion of the cells. Successful in vitro expansion of immune cells (e.g., T cells) is characterized by both sufficient cell expansion and the acquisition of a desired cell phenotype.

[0054] For example, T cell proliferation is characterized by the proliferation of naive T cells (T N ), memory T cells (stem cell-like memory (T SCM ), Central Memory (T CM ), and Effector Memory (T EM ) T cells, and effector (T EFF ) T cell mixtures containing T cell subsets. Varying amounts of different T cell subsets can affect the therapeutic profile and efficacy of the resulting engineered T cells. In particular, T cell expansion methods that result in enriched amounts of T cells at an early differentiation stage are therapeutically desirable.

[0055] Thus, the cell growth media described herein are SCM and / or T CM This may be particularly beneficial for T cell expansion, where a proportion of T SCM The cells are minimally differentiated memory T cells, which may be particularly advantageous for promoting long-term in vivo T cell proliferation following administration of the engineered cells to a patient for adoptive T cell therapy. Thus, such cell culture media and immune cells (e.g., T cells) prepared using such media may result in more potent adoptive cell transfer therapies, including CAR-T therapies described herein.

[0056] In particular, cell growth media containing combinations of different stimulants of cell growth (e.g., first and second T cell growth factors) in combination with extracellular regulators of cell metabolism can result in desirable cell growth and cell phenotypes.

[0057] In one aspect, the present disclosure provides a method for producing a method for manufacturing a semiconductor device comprising: a first stimulator of cell proliferation (e.g., a first cytokine that stimulates T cell proliferation); a second stimulator of cell proliferation (e.g., a second cytokine that stimulates T cell proliferation); A cell growth medium (e.g., a medium suitable for T cell growth) is featured that includes an extracellular regulator of cell metabolism (e.g., T cell metabolism).

[0058] In particular, the combinations and concentrations of cytokines and metabolic regulators described herein provide a novel T cell expansion medium and may result in increased efficacy of immune cell therapy products, including genetically modified allogeneic and autologous cell therapy products. In one embodiment, the combination of IL-7 and IL-15 stimulators with increased extracellular potassium enhances the proliferation and proliferation of allogeneic CAR T cells (e.g., T SCM In some cases, improvements in the acquisition of the desired phenotype of the autologous cell therapy product can be achieved.

[0059] 1.Immune cells Cells suitable for culture using the media and methods described herein include immune cells.

[0060] Prior to in vitro manipulation or genetic modification (e.g., as described herein), cells for use in the methods described herein (e.g., immune cells) can be obtained from a subject. Cells can be obtained from a number of non-limiting sources, including peripheral blood mononuclear cells, bone marrow, lymph node tissue, umbilical cord blood, thymus tissue, stem cell or iPSC-derived immune cells, tissue from an infection site, ascites, pleural effusion, spleen tissue, and tumors. In some embodiments, any number of T cell lines available and known to those skilled in the art can be used. In some embodiments, cells can be derived from healthy donors, patients diagnosed with cancer, or patients diagnosed with an infectious disease. In some embodiments, cells can be part of a mixed population of cells exhibiting different phenotypic characteristics.

[0061] In some embodiments, the immune cells are obtained from a subject who will ultimately receive the engineered immune cells, hi some embodiments, the immune cells are obtained from a donor, who is a different individual than the subject who will ultimately receive the engineered immune cells.

[0062] In some embodiments, the immune cells include T cells. T cells can be obtained from several sources, including peripheral blood mononuclear cells (PBMCs), bone marrow, lymph node tissue, umbilical cord blood, thymus tissue, stem cell or iPSC-derived T cells, tissue from an infection site, ascites, pleural effusion, spleen tissue, and tumor. In some specific embodiments, T cells can be obtained from a volume of blood drawn from a subject using any number of techniques known to those skilled in the art, such as FICOLL™ separation.

[0063] Cells can be obtained from the circulating blood of an individual by apheresis. Apheresis products typically contain lymphocytes, including T cells, monocytes, granulocytes, B cells, other nucleated white blood cells, red blood cells, and platelets. In certain embodiments, the cells collected by apheresis can be washed to remove plasma fraction and placed in a suitable buffer or medium for subsequent processing.

[0064] PBMCs can be used directly for genetic modification with immune cells (such as CAR or TCR) using the methods described herein. In certain embodiments, after isolating PBMCs, T lymphocytes can be further isolated and both cytotoxic and helper T lymphocytes can be sorted into naive, memory, and effector T cell subpopulations before or after genetic modification and / or expansion.

[0065] In certain embodiments, T cells are isolated from PBMCs by lysing red blood cells and depleting monocytes, for example, using centrifugation through a PERCOLL™ gradient. Specific subpopulations of T cells, such as CCR7+, CD95+, CD122, CD27+, CD69+, CD127+, CD28+, CD3+, CD4+, CD8+, CD25+, CD62L+, CD45RA+, and CD45RO+ T cells, can be further isolated by positive or negative selection techniques known in the art. For example, enrichment of T cell populations by negative selection can be achieved using a combination of antibodies directed against surface markers unique to the negatively selected cells. One method for use herein is cell sorting and / or selection via negative magnetic immunoadherence or flow cytometry using a cocktail of monoclonal antibodies directed against cell surface markers present on the negatively selected cells. For example, to enrich for CD4+ cells by negative selection, a monoclonal antibody cocktail typically includes antibodies to CD14, CD20, CD11b, CD16, HLA-DR, and CD8. Flow cytometry and cell sorting can also be used to isolate cell populations of interest for use in the present disclosure.

[0066] In some embodiments, the T cell population is enriched for C4+ cells.

[0067] In some embodiments, the T cell population is enriched for CD8+ cells.

[0068] In some embodiments, CD8+ cells are further sorted into naive, central memory, and effector cells by identifying cell surface antigens associated with each of these types of CD8+ cells. In some embodiments, the expression of phenotypic markers of central memory T cells includes CD45RO, CD62L, CCR7, CD28, CD3, and CD127, and is negative for granzyme B. In some embodiments, central memory T cells are CD45RO+, CD62L+, and / or CD8+ T cells. In some embodiments, effector T cells are negative for CD62L, CCR7, CD28, and / or CD127, and are positive for granzyme B and perforin. In certain embodiments, CD4+ T cells are further classified into subpopulations. For example, CD4+ T helper cells can be classified into naive, central memory, and effector cells by identifying cell populations with cell surface antigens.

[0069] It will be understood that PBMCs may further comprise other cytotoxic lymphocytes such as NK cells or NKT cells. An expression vector carrying the coding sequence of the chimeric receptor disclosed herein may be introduced into a population of human donor T cells, NK cells, or NKT cells. Successfully transduced T cells carrying the expression vector can be sorted using flow cytometry to isolate CD3 positive T cells, which can then be further expanded to increase the number of these CAR-expressing T cells in addition to cell activation using anti-CD3 antibodies and IL-2 or other methods known in the art as described elsewhere herein. Standard procedures are used for cryopreservation of CAR-expressing T cells for storage and / or preparation for use in human subjects. In one embodiment, in vitro transduction, culture, and / or expansion of T cells is performed in the absence of non-human animal derived products such as fetal calf serum and fetal bovine serum.

[0070] 2. Engineered immune cells The cell growth media and methods of use described herein may be particularly useful in the in vitro expansion of immune cells, including engineered immune cells (e.g., CAR-T cells).

[0071] The engineered immune cells can be allogeneic or autologous.

[0072] In some embodiments, the engineered immune cell is a T cell (e.g., inflammatory T lymphocytes cytotoxic T lymphocytes, regulatory T lymphocytes, helper T lymphocytes, tumor infiltrating lymphocytes (TILs)), NK cells, NK-T cells, TCR expressing cells, dendritic cells, killer dendritic cells, mast cells, or B cells. In some embodiments, the cells can originate from the group consisting of CD4+ T-lymphocytes and CD8+ T-lymphocytes. In some exemplary embodiments, the engineered immune cell is a T cell.

[0073] In some embodiments, the engineered immune cells may be derived from, for example but not limited to, stem cells. The stem cells may be adult stem cells, non-human embryonic stem cells, more particularly non-human stem cells, umbilical cord blood stem cells, progenitor cells, bone marrow stem cells, induced pluripotent stem cells, totipotent stem cells, or hematopoietic stem cells.

[0074] In some embodiments, the cells are obtained or prepared from peripheral blood. In some embodiments, the cells are obtained or prepared from peripheral blood mononuclear cells (PBMCs). In some embodiments, the cells are obtained or prepared from bone marrow. In some embodiments, the cells are obtained or prepared from umbilical cord blood. In some embodiments, the cells are human cells. In some embodiments, the cells are transfected or transduced with a nucleic acid vector using a method selected from the group consisting of electroporation, sonoporation, biolistics (e.g., Gene Gun), lipid transfection, polymer transfection, nanoparticles, or polyplexes.

[0075] a. Binding agents (including antibodies) In some embodiments, the engineered immune cells comprise an antigen binding agent (eg, comprise an antigen binding domain or comprise an antibody or fragment thereof).

[0076] As used herein, the term "antibody" refers to a polypeptide that contains sufficient standard immunoglobulin sequence elements to confer specific binding to a particular target antigen. As is known in the art, intact antibodies produced in nature are approximately 150 kD tetrameric agents consisting of two identical heavy chain polypeptides (about 50 kD each) and two identical light chain polypeptides (about 25 kD each) that associate with each other and bind in what is commonly referred to as a "Y-shaped" structure. Each heavy chain consists of at least four domains (each about 110 amino acids long) - an amino-terminal variable (VH) domain (located at the tip of the Y structure), followed by three constant domains, CHI, CH2, and carboxy-terminal CH3 (located at the base of the stem of the Y). A short region known as the "switch" connects the heavy chain variable and constant regions. A "hinge" connects the CH2 and CH3 domains to the rest of the antibody. Two disulfide bonds in this hinge region bind the two heavy chain polypeptides to each other in an intact antibody. Each light chain is composed of two domains, an amino-terminal variable (VL) domain followed by a carboxy-terminal constant (CL) domain, which are separated from each other by another "switch." Those skilled in the art are familiar with antibody structure and sequence elements and will recognize "variable" and "constant" regions in the sequences provided, and will understand that there may be some flexibility in the definition of the "boundaries" between such domains, such that different presentations of the same antibody chain sequence may exhibit such boundaries at positions that are shifted by, for example, one or a few residues relative to different presentations of the same antibody chain sequence.

[0077] An intact antibody tetramer consists of two heavy-light chain dimers, where the heavy and light chains are linked together by one disulfide bond and two other disulfide bonds link the heavy chain hinge regions together, linking the dimers together to form a tetramer. Naturally occurring antibodies are also glycosylated, typically on the CH2 domain. Each domain in a natural antibody has a structure characterized by an "immunoglobulin fold" formed from two beta sheets (e.g., 3-, 4-, or 5-stranded sheets) packed together in a compressed antiparallel beta barrel. Each variable domain contains three hypervariable loops known as "complement determining regions" (CDR1, CDR2, and CDR3) and four somewhat invariant "framework" regions (FR1, FR2, FR3, and FR4). When a natural antibody folds, the FR regions form a beta sheet that provides the structural framework of the domain, and the CDR loop regions from both the heavy and light chains come together in three-dimensional space to generate a single hypervariable antigen-binding site located at the tip of a Y structure. The Fc region of a naturally occurring antibody binds to elements of the complement system and also binds to receptors on effector cells, including effector cells that mediate cytotoxicity, for example. As is known in the art, the affinity and / or other binding properties of the Fc region for the Fc receptor can be modulated through glycosylation or other modifications. In some embodiments, the antibodies made and / or utilized according to the present disclosure include a glycosylated Fc domain, including Fc domains with modified or engineered such glycosylation.

[0078] For purposes of this disclosure, in certain embodiments, a polypeptide or complex of polypeptides that includes sufficient immunoglobulin domain sequences found in a natural antibody may be referred to and / or used as an "antibody," regardless of whether such a polypeptide is produced naturally (e.g., produced by an organism in response to an antigen) or produced by recombinant engineering, chemical synthesis, or other artificial systems or methodologies. In some embodiments, an antibody is polyclonal, and in some embodiments, an antibody is monoclonal. In some embodiments, an antibody has constant region sequences characteristic of mouse, rabbit, primate, or human antibodies. In some embodiments, the antibody sequence elements are humanized, primatized, chimeric, etc., as known in the art.

[0079] Furthermore, as used herein, the term "antibody" can refer, in appropriate embodiments, to any of the constructs or formats known or developed in the art for utilizing the structural and functional features of antibodies in alternative presentations (unless otherwise stated or clear from the context). For example, in some embodiments, antibodies utilized in accordance with the present disclosure include, but are not limited to, intact IgA, IgG, IgE or IgM antibodies; bispecific or multispecific antibodies (e.g., Zybodies®, etc.); antibody fragments such as Fab fragments, Fab' fragments, F(ab')2 fragments, Fd' fragments, Fd fragments, and isolated CDRs or sets thereof, single chain Fvs; polypeptide-Fc fusions; single domain antibodies (e.g., shark single domain antibodies such as IgNAR or fragments thereof); camelid antibodies; masked antibodies (e.g., Probodies®); Small Modular ImmunoPharmaceuticals ("SMIPs™"); single chain or tandem diabodies (TandAb®); VHH; Anticalins®; Nanobodies® minibodies; BiTEs®; Ankyrin repeat proteins or DARPIN®; Avimers®; DARTs; TCR-like antibodies;, Adnectins®; Affilins®; Trans-bodies®; Affibodies®; TrimerX®; Microproteins; Finomer®, Sentilin®; and KALBITOR®. In some embodiments, the antibody may lack a covalent modification (e.g., attachment of a glycan) that it has when naturally produced. In some embodiments, the antibody may contain a covalent modification (e.g., attachment of a glycan, a payload (e.g., a detectable moiety, a therapeutic moiety, a catalytic moiety, etc.), or other pendant group (e.g., polyethylene glycol, etc.)).

[0080] In some embodiments, an antibody or binding agent can be "symmetric." By "symmetric," we mean that the antibody or binding agent has the same type of Fv region (e.g., an antibody has two Fab regions). In some embodiments, an antibody or binding agent can be "asymmetric." By "asymmetric," we mean that the antibody or binding agent has at least two different types of Fv regions (e.g., an antibody has Fab and scFv regions, Fab and scFv2 regions, or Fab-VHH regions). A variety of asymmetric antibody or binding agent architectures are known in the art (Brinkman and Kontermann et al., 2017 Mabs(9)(2):182-212).

[0081] As used herein, the term "antibody agent" refers to an agent that specifically binds to a particular antigen. In some embodiments, the term encompasses any polypeptide or polypeptide complex that contains sufficient immunoglobulin structural elements to confer specific binding. Exemplary antibody agents include, but are not limited to, monoclonal or polyclonal antibodies. In some embodiments, an antibody can include one or more constant region sequences characteristic of mouse, rabbit, primate, or human antibodies. In some embodiments, an antibody agent can include one or more sequence elements that are humanized, primatized, chimeric, etc., as known in the art. In many embodiments, the term "antibody agent" is used to refer to one or more of the constructs or formats known or developed in the art to utilize the structural and functional features of antibodies in alternative presentations. For example, antibody agents utilized in accordance with the present disclosure include, but are not limited to, intact IgA, IgG, IgE or IgM antibodies; bispecific or multispecific antibodies (e.g., Zybodies®, etc.); antibody fragments such as Fab fragments, Fab' fragments, F(ab')2 fragments, Fd' fragments, Fd fragments, and isolated CDRs or sets thereof, single chain Fvs; polypeptide-Fc fusions; single domain antibodies (e.g., shark single domain antibodies such as IgNAR or fragments thereof); camelid antibodies; masked antibodies (e.g., Probodies®); Small Modular ImmunoPharmaceuticals ("SMIPs™"); single chain or tandem diabodies (TandAb®); VHH; Anticalins®; Nanobodies® minibodies; BiTEs®; Ankyrin repeat proteins or DARPIN®; Avimers®; DART; TCR-like antibodies; Adnectins®; Affilins®; Trans-bodies®; Affibodies®; TrimerX®; microproteins; Finomer®, Sentinilin®; and KALBITOR®.

[0082] The encoded antibody or antigen-binding molecule of the present disclosure may be single-chain or double-chain. In some embodiments, the antibody or antigen-binding molecule is single-chain. In some specific embodiments, the antigen-binding molecule is selected from the group consisting of scFv, Fab, Fab', Fv, F(ab')2, dAb, and any combination thereof.

[0083] Antibodies include antibody fragments. Antibody fragments include portions of an intact antibody, such as, for example, the antigen-binding or variable regions of the intact antibody. Antibody fragments include, but are not limited to, Fab, Fab', Fab'-SH, F(ab')2, Fv, diabodies, linear antibodies, antibody fragments formed from antibodies and multispecifics formed from scFv fragments, and other fragments described below. Antibodies also include, but are not limited to, polyclonal, monoclonal, chimeric dAb (domain antibodies), single chain, F ab , F a , F (ab ) 2 fragments, scFv, and F abThe antibody may be a whole antibody, or an immunoglobulin, or an antibody fragment. In some embodiments, the antibody is a full-length antibody, such as an intact IgGl antibody, or other antibody class or isotype described herein. (See, e.g., Hudson et al., Nat. Med., 9:129-134 (2003); Pluckthun, The Pharmacology of Monoclonal Antibodies, vol. 113, pp. 269-315 (1994); Hollinger et al., Proc. Natl. Acad. Sci. USA, 90:6444-6448 (1993); WO 93 / 01161; and U.S. Pat. Nos. 5,571,894, 5,869,046, 6,248,516, and 5,587,458.) Full-length, intact, or whole antibodies are antibodies having a structure substantially similar to a native antibody structure, or antibodies having heavy chains containing an Fc region as defined herein. Antibody fragments can be produced by a variety of techniques, as known in the art, including, but not limited to, proteolytic digestion of intact antibodies and production by recombinant host cells (e.g., E. coli or phages).

[0084] In some embodiments, the antibody is or comprises a monoclonal antibody, including a chimeric antibody, a humanized antibody, or a human antibody.

[0085] In some embodiments, the antigen-specific antibody agent provided herein may be a chimeric antibody (see, e.g., U.S. Pat. No. 4,816,567, and Morrison et al., Proc. Natl. Acad. Sci. USA, 81:6851-6855 (1984)). A chimeric antibody may be an antibody in which a portion of the heavy and / or light chain is derived from a particular source or species, while the remaining portion of the heavy and / or light chain is derived from a different source or species. In one example, a chimeric antibody may include a non-human variable region (e.g., a variable region derived from a mouse, rat, hamster, rabbit, or a non-human primate, such as a monkey) and a human constant region. In a further example, a chimeric antibody may be a "class-switched" antibody in which the class or subclass has been changed from that of the parent antibody. A chimeric antibody includes an antigen-binding fragment thereof.

[0086] In some embodiments, a chimeric antibody can be a humanized antibody (see, e.g., Almagro and Fransson, Front. Biosci., 13:1619-1633 (2008); Riechmann et al., Nature, 332:323-329 (1988); Queen et al., Proc. Natl Acad. Sci. USA 86:10029-10033 (1989); U.S. Patent Nos. 5,821,337, 7,527,791, 6,982,321, and 7,087,409; Kashmiri et al., Methods 36:25-34 (2005); Padlan, Mol. Immunol, 28:489-498 (1991); Dall'Acqua et al., Methods, 36:43-60 (2005); Osbourn et al., Methods, 36:61-68 (2005); and Klimka et al., Br. J. Cancer, 83:252-260 (2000). Humanized antibodies are chimeric antibodies that contain amino acid residues from a non-human hypervariable region and amino acid residues from a human FR. In certain embodiments, a humanized antibody contains substantially all of at least one, and typically two, variable domains, with all or substantially all of the hypervariable regions (e.g., CDRs) corresponding to those of a non-human antibody and all or substantially all of the FRs corresponding to those of a human antibody. A humanized antibody may optionally contain at least a portion of an antibody constant region derived from a human antibody.

[0087] A non-human antibody may be humanized to reduce immunogenicity to humans, while retaining the specificity and affinity of the parent non-human antibody. A humanized antibody may contain one or more variable domains that contain one or more CDRs, or portions thereof, from a non-human antibody. A humanized antibody may contain one or more variable domains that contain one or more FRs, or portions thereof, from a human antibody sequence. A humanized antibody may optionally contain at least a portion of a human constant region. In some embodiments, one or more FR residues in a humanized antibody are replaced with the corresponding residue from a non-human antibody (e.g., the antibody from which the CDR residues are derived) to restore or improve antibody specificity or affinity.

[0088] Human framework regions that may be used for humanization include, but are not limited to, framework regions selected using the "best fit" method; framework regions derived from the consensus sequence of human antibodies of a particular subpopulation of light or heavy chain variable regions; human mature (somatically mutated) framework regions or human germline framework regions; and framework regions (see, e.g., Sims et al., J. Immunol, 151:2296 (1993); Carter et al., Proc. Natl. Acad. Sci. USA, 89:4285 (1992); Presta et al., J. Immunol, 151:2623 (1993); Baca et al., J. Biol. Chem., 272:10678-10684 (1997); and Rosok et al., J. Biol. Chem., 271:22611-22618 (1996)).

[0089] In some embodiments, the antibody agents provided herein are human antibodies. Human antibodies can be made using a variety of techniques known in the art (see, e.g., van Dijk and van de Winkel, Curr. Opin. Pharmacol, 5:368-74 (2001); Lonberg, Curr. Opin. Immunol, 20:450-459 (2008). A human antibody may have an amino acid sequence that corresponds to the amino acid sequence of an antibody produced by a human or human cell, or derived from a non-human source that utilizes a human antibody repertoire or other human antibody coding sequence. This definition of a human antibody specifically excludes humanized antibodies, which contain non-human antigen-binding residues. A human antibody is an antibody that produces intact human antibodies or human variable regions in response to antigen challenge. Human variable regions from intact antibodies produced by such animals can be further modified, for example, by combining with a different human constant region.

[0090] Human antibodies may also be made by hybridoma-based methods. For example, human antibodies can be produced from human myeloma and mouse-human heteromyeloma cell lines using human B cell hybridoma technology, and other methods (see, e.g., Kozbor, J. Immunol, 133:3001 (1984); Brodeur et al., Monoclonal Antibody Production Techniques and Applications, pp. 51-63 (1987); Boerner et al., J. Immunol, 147:86 (1991); Li et al., Proc. Natl. Acad. Sci. USA, 103:3557-3562 (2006); U.S. Patent No. 7,189,826; Ni, Xiandai Mianyixue, 26(4):265-268 (2006); Vollmers and Brandlein, Histology and Histopathology, 20(3):927-937 (2005); and Vollmers and Brandlein, Methods and (See, Findings in Experimental and Clinical Pharmacology, 27(3):185-91 (2005)). Human antibodies can also be produced by isolating Fv clone variable domain sequences selected from human-derived phage display libraries. Such variable domain sequences can then be combined with the desired human constant regions.

[0091] Modification of the oligosaccharides in the antibody can be made, for example, to generate antibody variants with specific improved properties. For example, antibody glycosylation variants can have improved CDC function. In some embodiments, the present disclosure contemplates antibody variants that have some, but not all, effector functions, making them desirable candidates for applications where the in vivo half-life of the antibody is important, but certain effector functions (such as complement) are unnecessary or detrimental. In vitro and / or in vivo cytotoxicity assays can be performed to confirm the reduction / depletion of CDC activity.

[0092] In some embodiments, the antibody agents provided herein may be further modified to contain additional non-proteinaceous moieties that are known in the art and readily available. Moieties suitable for derivatization of antibodies may include, but are not limited to, water-soluble polymers. Non-limiting examples of water-soluble polymers include, but are not limited to, polyethylene glycol (PEG), ethylene glycol / propylene glycol copolymers, carboxymethylcellulose, dextran, polyvinyl alcohol, polyvinylpyrrolidone, poly-1,3-dioxolane, poly-1,3,6-trioxane, ethylene / maleic anhydride copolymers, polyamino acids (either homopolymers or random copolymers), and dextran or poly(n-vinylpyrrolidone) polyethylene glycol, polypropylene glycol homopolymer, polypropylene oxide / ethylene oxide copolymer, polyoxyethylated polyols (e.g., glycerol), polyvinyl alcohol, and mixtures thereof. Polyethylene glycol propionaldehyde may have advantages in manufacturing due to its stability in water.

[0093] The polymer may be of any molecular weight, and may be branched or unbranched. The number of polymers attached to the antibody may vary, and when more than one polymer is attached, they may be the same or different molecules.

[0094] In some embodiments, a conjugate of an antibody and a non-proteinaceous moiety is provided that can be selectively heated by exposure to radiation. In some embodiments, the non-proteinaceous moiety can be a carbon nanotube (see, for example, Kam et al., Proc.Natl.Acad.Sci.USA, 102:11600-11605 (2005)). The radiation can be of any wavelength, including but not limited to wavelengths that are not harmful to normal cells, but heat the non-proteinaceous moiety to a temperature that kills cells in the vicinity of the antibody-non-proteinaceous moiety.

[0095] B chimeric antigen receptor In some embodiments, the engineered immune cells comprise a population of CARs, each of which comprises an extracellular antigen binding domain. In some embodiments, the engineered immune cells comprise a population of CARs, each of which comprises an extracellular antigen binding domain.

[0096] As used herein, a chimeric antigen receptor (CAR) is a protein that specifically recognizes a target antigen (e.g., a target antigen on a cancer cell). Upon binding to a target antigen, the CAR can activate immune cells to attack and destroy cells (e.g., cancer cells) that bear that antigen. CARs can also incorporate costimulatory or signaling domains to increase their efficacy. See Krause et al., J. Exp. Med., Volume 188, No. 4, 1998 (619-626); Finney et al., Journal of Immunology, 1998, 161:2791-2797; Song et al., Blood 119:696-706 (2012); Kalos et al., Sci. Transl. Med. 3:95 (2011); Porter et al., N. Engl. J. Med. 365:725-33 (2011), and Gross et al., Annu. Rev. Pharmacol. Toxicol. 56:59-83 (2016); U.S. Patent Nos. 7,741,465 and 6,319,494.

[0097] The chimeric antigen receptors described herein comprise an extracellular domain, a transmembrane domain, and an intracellular domain, and the extracellular domain comprises an antigen binding domain that specifically binds to a target.

[0098] In some embodiments, the antigen-specific CAR further comprises a safety switch and / or one or more monoclonal antibody specific epitopes.

[0099] i. Antigen-binding domain As mentioned above, the CAR described herein comprises an antigen-binding domain. As used herein, "antigen-binding domain" refers to any polypeptide that binds to a specified target antigen. In some embodiments, the antigen-binding domain binds to an antigen on a tumor cell. In some embodiments, the antigen-binding domain binds to an antigen on a cell involved in a hyperproliferative disease.

[0100] In some embodiments, the antigen-binding domain comprises a variable heavy chain, a variable light chain, and / or one or more CDRs described herein. In some embodiments, the antigen-binding domain is a single chain variable fragment (scFv) comprising CDR1, CDR2, and CDR3 of the light chain CDRs, and CDR1, CDR2, and CDR3 of the heavy chain CDRs.

[0101] Variants of the antigen-binding domain (e.g., variants of the CDR, VH and / or VL) are also within the scope of the present disclosure, such as variable light chains and / or variable heavy chains, each having at least 70-80%, 80-85%, 85-90%, 90-95%, 95-97%, 97-99%, or more than 99% identity with the amino acid sequence of the antigen-binding domain sequence. In some examples, such molecules include at least one heavy chain and one light chain, while in other examples, the variant forms include two variable light chains and two variable heavy chains (or subportions thereof). Those of skill in the art can determine suitable variants of the antigen-binding domains described herein using well-known techniques. In certain embodiments, those of skill in the art can identify suitable regions of the molecule that can be altered without destroying activity by targeting regions not believed to be important for activity.

[0102] In certain embodiments, the polypeptide structure of the antigen-binding domain is based on an antibody, including, but not limited to, a monoclonal antibody, a bispecific antibody, a minibody, a domain antibody, a synthetic antibody (sometimes referred to herein as an "antibody mimetic"), a chimeric antibody, a humanized antibody, a human antibody, an antibody fusion (sometimes referred to herein as an "antibody conjugate"), and fragments thereof, respectively. In some embodiments, the antigen-binding domain comprises or consists of an avimer.

[0103] An antigen-binding domain is said to be "selective" if it binds more strongly to one target than it binds to a second target.

[0104] In some embodiments, the antigen binding domain is an scFv.

[0105] In some embodiments, the antigen-selective CAR comprises a leader or signal peptide.

[0106] In other embodiments, the disclosure relates to an isolated polynucleotide encoding any one of the antigen binding domains described herein. In some embodiments, the disclosure relates to an isolated polynucleotide encoding a CAR. Also provided herein are vectors comprising the polynucleotides and methods of making the polynucleotides.

[0107] ii. Safety Switch and Monoclonal Antibody Specific Epitope It is understood that adverse events can be minimized by transducing immune cells (containing one or more CARs) with suicide genes. It may also be desirable to incorporate an inducible "on" or "facilitator" switch into immune cells. Suitable techniques include the use of inducible caspase-9 (US Appl. 2011 / 0286980) or thymidine kinase before, after, or at the same time that cells are transduced with the CAR constructs of the present disclosure. Additional methods for introducing suicide genes and / or "on" switches include TALENS, zinc fingers, RNAi, siRNA, shRNA, antisense technology, and other techniques known in the art.

[0108] According to the present disclosure, additional on-off or other types of control switch technologies can be incorporated herein. These technologies can employ the use of dimerization domains and any activator of such domain dimerization. These technologies include, for example, those described by Wu et al., Science 2014 350(6258), which utilizes the FKBP / rapalog dimerization system in certain cells, the contents of which are incorporated herein by reference in their entirety. Additional dimerization technologies are described, for example, in Fegan et al. Chem.Rev.2010, 110, 3315-3336, and U.S. Patent Nos. 5,830,462, 5,834,266, 5,869,337, and 6,165,787, the contents of which are also incorporated herein by reference in their entirety. Additional dimerization pairs may include cyclosporine-A / cyclophilin, receptor, estrogen / estrogen receptor (optionally using tamoxifen), glucocorticoid / glucocorticoid receptor, tetracycline / tetracycline receptor, vitamin D / vitamin D receptor. Further examples of dimerization techniques are described, for example, in WO2014 / 127261, WO2015 / 090229, US2014 / 0286987, US2015 / 0266973, US2016 / 0046700, U.S. Patent No. 8,486,693, US2014 / 0171649, and US2012 / 0130076, the contents of which are incorporated herein by reference in their entireties.

[0109] In some embodiments, the CAR immune cells (e.g., CAR-T cells) of the present disclosure comprise a polynucleotide encoding a suicide polypeptide, such as, for example, RQR8. See, for example, WO2013153391A, incorporated herein by reference in its entirety. In the CAR immune cells (e.g., CAR-T cells) comprising the polynucleotide, the suicide polypeptide is expressed on the surface of the CAR immune cells (e.g., CAR-T cells). In some embodiments, the suicide polypeptide is SEQ ID NO:1. It contains the amino acid sequence shown in CPYSNPSLCSGGGGSELPTQGTFSNVSTNVSPAKPTTTACPYSNPSLCSGGGGSP APRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLS LVITLYCNHRNRRRVCKCPRPVV (SEQ ID NO: 1).

[0110] The suicide polypeptide may also include a signal peptide at the amino terminus, such as MGTSLLCWMALCLLGADHADA (SEQ ID NO: 2). In some embodiments, the suicide polypeptide includes the amino acid sequence shown in SEQ ID NO: 3, and includes the signal sequence of SEQ ID NO: 2.

[0111] MGTSLLCWMALCLLGADHADACPYSNPSLCSGGGGSELPTQGTFSNVSTNVSPAKPTTTACPYSNPSLCSGGGGSPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLYCNHRNRRRVCKCPRPVV (SEQ ID NO: 3).

[0112] When the suicide polypeptide is expressed on the surface of a CAR immune cell (e.g., a CAR-T cell), lysis of the cell occurs when rituximab binds to the R epitope of the polypeptide. Multiple molecules of rituximab can be bound per polypeptide expressed on the cell surface. Each R epitope of the polypeptide can bind to a separate rituximab molecule. Deletion of antigen-specific CAR immune cells (e.g., CAR-T cells) can occur in vivo, for example, by administering rituximab to a patient. The decision to delete the introduced cells can result from undesirable effects detected in the patient due to the introduced cells, for example, when an unacceptable level of toxicity is detected.

[0113] In some embodiments, the suicide polypeptide is expressed on the surface of the cell. In some embodiments, the suicide polypeptide is included in a CAR construct. In some embodiments, the suicide polypeptide is not part of a CAR construct.

[0114] In some embodiments, the extracellular domain of an antigen-specific CAR may contain one or more epitopes specific for (i.e., specifically recognized by) a monoclonal antibody. These epitopes are also referred to herein as mAb-specific epitopes. Examples of mAb-specific epitopes are disclosed in International Patent Publication No. WO2016 / 120216, which is incorporated herein in its entirety. In these embodiments, the extracellular domain of a CAR comprises an antigen-binding domain that specifically binds to an antigen and one or more epitopes that bind to one or more monoclonal antibodies (mAbs). A CAR that contains a mAb-specific epitope may be single-chain or multi-chain.

[0115] The inclusion of an epitope specific for a monoclonal antibody in the extracellular domain of a CAR described herein allows for sorting and depletion of engineered immune cells expressing a CAR. In some embodiments, this function also facilitates the recovery of endogenous antigen-expressing cells that are depleted by administration of engineered immune cells expressing a CAR. In some embodiments, allowing depletion provides a safety switch in the event of adverse effects, for example, upon administration to a subject.

[0116] Thus, in some embodiments, the present disclosure relates to methods for sorting and / or depleting engineered immune cells endowed with CARs containing mAb-specific epitopes, as well as methods for promoting recovery of endogenous antigen-expressing cells.

[0117] Some epitope-monoclonal antibody conjugates can be used to generate CARs that contain monoclonal antibody specific epitopes, particularly CARs that are already approved for medical use, such as, as a non-limiting example, CD20 epitope / rituximab.

[0118] The antigen also encompasses methods of sorting engineered immune cells endowed with antigen-specific CARs expressing mAb-specific epitopes, and therapeutic methods in which activation of engineered immune cells endowed with these CARs is modulated by depleting the cells using antibodies that target the external ligand-binding domain of the CARs. Table 1 provides exemplary mimotope sequences that can be inserted into the extracellular domain of any one of the CARs of the present disclosure. [Table 1]

[0119] In some embodiments, the extracellular binding domain of the CAR comprises the following sequence: V 1- L 1- V 2- (L) x -Epitope 1-(L) x -; V 1- L 1- V 2- (L) x -Epitope 1-(L) x -Epitope 2-(L) x -; V 1- L 1- V 2- (L) x -Epitope 1-(L) x -Epitope 2-(L) x -Epitope 3-(L) x -; (L) x -Epitope 1-(L) x -V 1- L 1- V2; (L) x -Epitope 1-(L)x -Epitope 2-(L) x -V 1- L 1- V2; Epitope 1(L) x -Epitope 2-(L) x -Epitope 3-(L) x -V 1- L 1- V2; (L) x -Epitope 1-(L) x -V 1- L 1- V 2- (L) x -Epitope 2-(L) x ; (L) x -Epitope 1-(L) x -V 1- L 1- V 2- (L) x -Epitope 2-(L) x -Epitope 3-(L) x -; (L) x -Epitope 1-(L) x -V 1- L 1- V 2- (L) x -Epitope 2-(L) x -Epitope 3-(L) x -Epitope 4-(L) x -; (L) x -Epitope 1-(L) x -Epitope 2-(L) x -V 1- L 1- V 2- (L) x -Epitope 3-(L) x -; (L) x -Epitope 1-(L) x -Epitope 2-(L) x -V 1- L 1- V 2- (L) x -Epitope 3-(L) x -Epitope 4-(L)x -; V 1- (L) x -Epitope 1-(L) x -V2; V 1- (L) x -Epitope 1-(L) x -V 2- (L) x -Epitope 2-(L) x ; V 1- (L) x -Epitope 1-(L) x -V 2- (L) x -Epitope 2-(L) x -Epitope 3-(L) x ; V 1- (L) x -Epitope 1-(L) x -V 2- (L) x -Epitope 2-(L) x -Epitope 3-(L) x -Epitope 4-(L) x ; (L) x -Epitope 1-(L) x -V 1- (L) x -Epitope 2-(L) x -V2; or (L) x -Epitope 1-(L) x -V 1- (L) x -Epitope 2-(L) x -V 2- (L) x -Epitope 3-(L) x ; During the ceremony, V1 is V L and V2 is V H or V1 is V H and V2 is V L and L1 is V H Chain V L is a linker suitable for linking to a chain, Epitope 1, epitope 2, epitope 3, and epitope 4 are mAb-specific epitopes, which may be the same or different, and V H is the heavy chain variable fragment, and V L is the light chain variable fragment.

[0120] iii. Hinge domain The extracellular domain of the CAR of the present disclosure may comprise a "hinge" domain (or hinge region). This term generally includes any polypeptide that functions to link the transmembrane domain in the CAR to the extracellular antigen-binding domain in the CAR. In particular, the hinge domain can be used to provide more flexibility and accessibility to the extracellular antigen-binding domain.

[0121] The hinge domain can comprise up to 300 amino acids, in some embodiments 10-100 amino acids, or in some embodiments 25-50 amino acids. The hinge domain can originate from all or part of a naturally occurring molecule derived from CD8, CD4, CD28, 4-1BB, or the extracellular region of IgG (particularly understood to include all or part of the hinge region of IgG, i.e., some or all of the members of immunoglobulins such as IgG1, IgG2, IgG3, IgG4, IgA, IgD, IgE, IgM, or fragments thereof) or all or part of an antibody heavy chain constant region. Alternatively, the A domain may be a synthetic sequence corresponding to a naturally occurring A sequence, or may be a completely synthetic A sequence. In some embodiments, said A domain is part of the human CD8 α chain (e.g., NP_001139345.1). In another particular embodiment, said hinge domain and transmembrane domain comprise a part of the human CD8 α chain. In some embodiments, the hinge domain of the CAR described herein comprises a subsequence of CD8α, IgG1, IgG4, PD-1, or FcγRIIIα, in particular the hinge region of either CD8α, IgG1, IgG4, PD-1, or FcγRIIIα. In some embodiments, the hinge domain comprises a human CD8α hinge, a human IgG1 hinge, a human IgG4, a human PD-1, or a human FcγRIIIα hinge. In some embodiments, the CAR disclosed herein comprises an scFv, a CD8α human hinge and transmembrane domain, a CD3ζ signaling domain, and a 4-1BB signaling domain. Table 2 provides the amino acid sequences of exemplary hinges provided herein. [Table 2]

[0122] iv. Transmembrane domain The CAR of the present disclosure is designed with a transmembrane domain fused to the extracellular domain of the CAR. This can also be fused to the intracellular domain of the CAR. In some examples, the transmembrane domain can be selected or modified by amino acid substitution to avoid the binding of such domain to the transmembrane domain of the same or different surface membrane protein and minimize the interaction with other members of the receptor complex. In some embodiments, a short linker can form a bond between any or part of the extracellular domain, transmembrane domain, and intracellular domain of the CAR.

[0123] Suitable transmembrane domains of the CARs disclosed herein include (a) transmembrane domains that are capable of translocating, for example, T helper (T h ) cells, cytotoxic T(T c ) cells, T regulatory (T reg ) the ability to be expressed on the surface of an immune cell, such as a target cell, or a lymphocyte cell, such as a natural killer (NK) cell, and / or (b) the ability to interact with an extracellular antigen-binding domain and an intracellular signaling domain to induce a cellular response of the immune cell to direct a cellular response of the immune cell against a target cell.

[0124] The transmembrane domain may be derived from either natural or synthetic sources. If the source is natural, the domain may be derived from any membrane-bound or transmembrane protein.

[0125] Transmembrane regions of particular use in the present disclosure include those derived from CD28, OX-40, 4-1BB / CD137, CD2, CD7, CD27, CD30, CD40, programmed death-1 (PD-1), inducible T cell costimulator (ICOS), lymphocyte function associated antigen-1 (LFA-1, CD1-1a / CD18), CD3 gamma, CD3 delta, CD3 epsilon, CD247, CD276 (B7-H3), LIGHT, (TNFSF14), NKG2C, Ig alpha (CD79a), DAP-10, Fc gamma receptor, MHC class 1 molecule, TNF receptor protein, immunoglobulin protein, cytokine receptor, IgA, IgAg, IgBg ... Integrin, signaling lymphocyte activation molecule (SLAM protein), activating NK cell receptor, BTLA, Toll ligand receptor, ICAM-1, B7-H3, CDS, ICAM-1, GITR, BAFFR, LIGHT, HVEM (LIGHTR), KIRDS2, SLAMF7, NKp80 (KLRF1), NKp44, NKp30, NKp46, CD19, CD4, CD8 alpha, CD8 beta, IL-2R beta, IL-2R gamma, IL-7R alpha, ITGA4, VLA1, CD49a, ITGA4, IA4, CD49D, ITGA6, VLA-6, CD49f, ITGAD, CD1 1d, ITGAE, CD103, ITGAL, CD1 1a, LFA-1, ITGAM, CD1 1b, ITGAX, CD1 1c, ITGB1, CD29, ITGB2, CD18, LFA-1, ITGB7, NKG2D, TNFR2, TRANCE / RANKL, DNAM1(CD226), SLAMF4(CD244, 2B4), CD84, CD96(Tactile), CEACAM1, CRT and a ligand that specifically binds to AM, Ly9 (CD229), CD160 (BY55), PSGL1, CD100 (SEMA4D), CD69, SLAMF6 (NTB-A, Ly108), SLAM (SLAMF1, CD150, IPO-3), BLAME (SLAMF8), SELPLG (CD162), LTBR, ​​LAT, GADS, SLP-76, PAG / Cbp, CD19a, CD83, or any combination thereof.

[0126] As non-limiting examples, the transmembrane region may be derived from, for example, the α, β, γ or δ polypeptides constituting the CD3 complex, the IL-2 receptor p55 (a chain), p75 (β chain), or γ chain, subunit chains of the Fc receptor, or may be part of a T cell receptor, particularly in the Fcγ receptor III or CD protein. Alternatively, the transmembrane domain may be synthetic and may comprise primarily hydrophobic residues such as leucine and valine. In some embodiments, the transmembrane domain is derived from the human CD8 α chain (e.g., NP_001139345.1).

[0127] In some embodiments, the transmembrane domain in a CAR of the present disclosure is a CD8α transmembrane domain. In some embodiments, the transmembrane domain in a CAR of the present disclosure is a CD8α transmembrane domain comprising the amino acid sequence IYIWAPLAGTCGVLLLSLVIT (SEQ ID NO: 17). In some embodiments, the CD8α transmembrane domain comprises a nucleic acid sequence encoding the transmembrane amino acid sequence of SEQ ID NO: 17. In some embodiments, the hinge and transmembrane domain in a CAR of the present disclosure is a CD8α hinge and transmembrane domain comprising the amino acid sequence of SEQ ID NO: 17.

[0128] In some embodiments, the transmembrane domain in the CAR of the present disclosure is a CD28 transmembrane domain. In some embodiments, the transmembrane domain in the CAR of the present disclosure is a CD28 transmembrane domain comprising the amino acid sequence FWVLVVVGGVLACYSLLVTVAFIIFWV (SEQ ID NO: 18). In some embodiments, the CD28 transmembrane domain comprises a nucleic acid sequence encoding the transmembrane amino acid sequence of SEQ ID NO: 18.

[0129] v. Intracellular domain The intracellular (cytoplasmic) domain of the CAR of the present disclosure can provide for activation of at least one normal effector function of an immune cell that contains a CAR. Effector function of a T cell can refer to, for example, cytolytic activity, including secretion of cytokines, or helper activity.

[0130] In some embodiments, an activating intracellular signaling domain for use in a CAR may be, for example, but is not limited to, the cytoplasmic sequences of the T cell receptor and co-receptors that act in concert to initiate signaling following antigen receptor engagement, as well as any derivatives or variants of these sequences, and any synthetic sequences that have the same function.

[0131] Suitable (e.g., activation) intracellular domains include, but are not limited to, CD28, OX-40, 4-1BB / CD137, CD2, CD7, CD27, CD30, CD40, programmed death-1 (PD-1), inducible T cell costimulator (ICOS), lymphocyte function associated antigen-1 (LFA-1, CD1-1a / CD18), CD3 gamma, CD3 delta, CD3 epsilon, CD247, CD276 (B7-H3), LIGHT, (TNFSF14), NKG2C, Ig alpha (CD79a), DAP-10, Fc gamma receptor, MHC class 1 molecule, TNF receptor protein, immunoglobulin protein, cytokines, Receptor, Integrin, Signaling Lymphocyte Activation Molecule (SLAM Protein), Activating NK Cell Receptor, BTLA, Toll Ligand Receptor, ICAM-1, B7-H3, CDS, ICAM-1, GITR, BAFFR, LIGHT, HVEM (LIGHTR), KIRDS2, SLAMF7, NKp80 (KLRF1), NKp44, NKp30, NKp46, CD19, CD4, CD8 alpha, CD8 beta, IL-2R beta, IL-2R gamma, IL-7R alpha, ITGA4, VLA1, CD49a, ITGA4, IA4, CD49D, ITGA6, VLA-6, CD49f, ITGAD, CD1 1d, ITGAE, CD103, ITGAL, CD1 1a, LFA-1, ITGAM, CD1 1b, ITGAX, CD1 1c, ITGB1, CD29, ITGB2, CD18, LFA-1, ITGB7, NKG2D, TNFR2, TRANCE / RANKL, DNAM1(CD226), SLAMF4(CD244, 2B4), CD84, CD96(Tactile), CEACAM1, CRT The signaling domains include those derived from (or corresponding to) a ligand that specifically binds to AM, Ly9 (CD229), CD160 (BY55), PSGL1, CD100 (SEMA4D), CD69, SLAMF6 (NTB-A, Ly108), SLAM (SLAMF1, CD150, IPO-3), BLAME (SLAMF8), SELPLG (CD162), LTBR, ​​LAT, GADS, SLP-76, PAG / Cbp, CD19a, CD83, or any combination thereof.

[0132] In addition to the activation domains described above, the intracellular domains of the CARs of the present disclosure can incorporate costimulatory signaling domains (interchangeably referred to herein as costimulatory molecules) to increase their efficacy. The costimulatory domains can provide signals in addition to the primary signals provided by the activation molecules described herein.

[0133] Suitable costimulatory domains within the scope of the present disclosure include, for example, CD28, OX40, 4-1BB / CD137, CD2, CD3 (alpha, beta, delta, epsilon, gamma, zeta), CD4, CD5, CD7, CD9, CD16, CD22, CD27, CD30, CD 33, CD37, CD40, CD45, CD64, CD80, CD86, CD134, CD137, CD154, PD-1, ICOS, lymphocyte function associated antigen-1 (LFA-1 (CD1 1a / CD18), CD247, CD276 (B7-H3), LIGHT (tumor necrosis factor superfamily member 14; TNFSF14), NKG2C, Ig alpha (CD79a), DAP-10, Fc gamma receptor, MHC class I molecule, TNFR, integrin, signaling lymphocyte activation molecule, BTLA, Toll ligand receptor, ICAM-1, B7-H3, CDS, ICAM-1, GITR, BAFFR, LIGHT, HVEM (LIGHTR), KIRDS2, SLAMF7, NKp80 (KLRF1), NKp44, NKp30, NKp46, CD19, CD4, CD8 alpha, CD 8beta, IL-2Rbeta, IL-2Rgamma, IL-7Ralpha, ITGA4, VLA1, CD49a, ITGA4, IA4, CD49D, ITGA6, VLA-6, CD49f, ITGAD, CD1-1d, ITGAE, CD103, ITGAL, CD1-1a, LFA-1, ITGAM, CD1 -1b, ITGAX, CD1-1c, ITGB1, CD29, ITGB2, CD18, LFA-1, ITGB7, NKG2D, TNFR2, TRANCE / RANKL, DNAM1(CD226), SLAMF4(CD244, 2B4), CD84, CD96(Tactile), CEACAM1, CRT It is understood that the co-stimulatory molecules may be derived from AM, Ly9 (CD229), CD160 (BY55), PSGL1, CD100 (SEMA4D), CD69, SLAMF6 (NTB-A, Ly108), SLAM (SLAMF1, CD150, IPO-3), BLAME (SLAMF8), SELPLG (CD162), LTBR, ​​LAT, GADS, SLP-76, PAG / Cbp, CD19a, CD83 ligand, or fragments or combinations thereof. It is understood that additional co-stimulatory molecules or fragments thereof not listed above are within the scope of the present disclosure.

[0134] In some embodiments, the intracellular / cytoplasmic domain of the CAR may be designed to include the 4-1BB / CD137 domain by itself or may be combined with any other desired intracellular domain useful in the context of the CAR of the present disclosure. The complete native amino acid sequence of 4-1BB / CD137 is set forth in NCBI Reference Sequence: NP_001552.2. The complete native 4-1BB / CD137 nucleic acid sequence is set forth in NCBI Reference Sequence: NM_ 001561.5.

[0135] In some embodiments, the intracellular / cytoplasmic domain of the CAR may be designed to include the CD28 domain by itself or may be combined with any other desired intracellular domain useful in the context of the CAR of the present disclosure. The complete native amino acid sequence of CD28 is described in NCBI Reference Sequence: NP_006130.1. The complete native CD28 nucleic acid sequence is described in NCBI Reference Sequence: NM_006139.1.

[0136] In some embodiments, the intracellular / cytoplasmic domain of the CAR may be designed to include a CD3 zeta domain by itself or in combination with any other desired intracellular domain useful in the context of the CARs of the present disclosure. In some embodiments, the intracellular signaling domain of the CAR may include a CD3 zeta signaling domain having an amino acid sequence having at least about 70%, at least 80%, at least 90%, 95%, 97%, or 99% sequence identity to the amino acid sequence set forth in SEQ ID NO: 19 or SEQ ID NO: 26. LRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR (SEQ ID NO: 19) LRVKFSRSADAPAYKQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR (SEQ ID NO: 26) For example, the intracellular domain of the CAR may comprise a portion of a CD3 zeta chain and a portion of a costimulatory signaling molecule. The intracellular signaling sequences in the intracellular signaling portion of the CAR of the present disclosure can be linked together randomly or in a specific order. In some embodiments, the intracellular domain is designed to comprise the activation domain of CD3 zeta and the signaling domain of CD28. In some embodiments, the intracellular domain is designed to comprise the activation domain of CD3 zeta and the signaling domain of 4-1BB.

[0137] In some embodiments, 4-1BB (the intracellular domain) comprises the amino acid sequence. KRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCEL (SEQ ID NO: 20). In some embodiments, 4-1BB (intracellular domain) is encoded by the nucleic acid sequence: AAGCGCGGGCAGGAAGAAGCTCCTCTACATTTTTAAGCAGCCTTTTATGAGGCCCGTACAGACAACACAGGAGGAAGATGGCTGTAGCTGCAGATTTCCCGAGGAGGAGGAAGGTGGGTGCGAGCTG (SEQ ID NO: 21).

[0138] In some embodiments, the intracellular domain in the CAR is designed to comprise a portion of CD28 and CD3 zeta, wherein the intracellular CD28 comprises the nucleic acid sequence set forth in SEQ ID NO:22. AGATCCAAAAGAAGCCGCCTGCTCCATAGCGATTACATGAATATGACTCC ACGCCGCCCTGGCCCCACAAGGAAACACTACCAGCCTTACGCACCACCTAGAGATTTCGCTGCCTATCGGAGC (SEQ ID NO: 22).

[0139] In some embodiments, the intracellular domain in the CAR is designed to comprise the amino acid sequence RSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPPRDFAAYRS (SEQ ID NO: 23). The CD3 zeta amino acid sequence can comprise SEQ ID NO:23 and the nucleic acid sequence can comprise SEQ ID NO:24. AGGGTGAAGTTTTCCAGATCTGCAGATGCACCAGCGTATCAGCAGGGCCAGAACCAACTGTATAACGAGCTCAACCTGGGACGCAGGGAAGAGTATGACGTTTTGGACAAGCGCAGAGGACGGGACCCTGAGATGGGTGGCAAACCAAGACGAAAAAACCCCCAGGAGGGTC TCTATAATGAGCTGCAGAAGGATAAGATGGCTGAAGCCTATTCTGAAATAGGCATGAAAGGAGAGCGGAGAAGGGGAAAAGGGCACGACGGTTTGTACCAGGGACTCAGCACTGCTACGAAGGATACTTATGACGCTCTCCACATGCAAGCCCTGCCACCTAGG (SEQ ID NO: 24).

[0140] In some embodiments, the intracellular signaling domain of a CAR of the present disclosure comprises a domain of a costimulatory molecule. In some embodiments, the intracellular signaling domain of a CAR of the present disclosure comprises a portion of a costimulatory molecule selected from the group consisting of fragments of 4-1BB (GenBank: AAA53133.) and CD28 (NP_006130.1). In some embodiments, the intracellular signaling domain of a CAR comprises an amino acid sequence that comprises at least 70%, at least 80%, at least 90%, 95%, 97%, or 99% sequence identity to the amino acid sequence set forth in SEQ ID NO:20 and SEQ ID NO:22. In some embodiments, the intracellular signaling domain of a CAR of the present disclosure comprises an amino acid sequence that comprises at least 70%, at least 80%, at least 90%, 95%, 97%, or 99% sequence identity to the amino acid sequence set forth in SEQ ID NO:20 and / or at least 70%, at least 80%, at least 90%, 95%, 97%, or 99% sequence identity to the amino acid sequence set forth in SEQ ID NO:22.

[0141] c. Immune cells containing CAR Provided herein are engineered immune cells that express a CAR of the disclosure (e.g., CAR-T cells).

[0142] In some embodiments, the engineered immune cells comprise a population of CARs, each CAR comprising an extracellular antigen binding domain. In some embodiments, the engineered immune cells comprise a population of CARs, each CAR comprising a different extracellular antigen binding domain. In some embodiments, the immune cells comprise a population of CARs, each CAR comprising an extracellular antigen binding domain.

[0143] The engineered immune cells can be allogeneic or autologous.

[0144] In some embodiments, the engineered immune cell is a T cell (e.g., inflammatory T lymphocytes cytotoxic T lymphocytes, regulatory T lymphocytes, helper T lymphocytes, tumor infiltrating lymphocytes (TIL)), NK cell, NK-T cell, TCR expressing cell, dendritic cell, killer dendritic cell, mast cell, or B cell. In some embodiments, the cell can originate from the group consisting of CD4+ T-lymphocytes and CD8+ T-lymphocytes. In some exemplary embodiments, the engineered immune cell is a T cell. In some exemplary embodiments, the engineered immune cell is a gamma delta T cell. In some exemplary embodiments, the engineered immune cell is a macrophage.

[0145] In some embodiments, the engineered immune cells may be derived from, for example but not limited to, stem cells. The stem cells may be adult stem cells, non-human embryonic stem cells, more particularly non-human stem cells, umbilical cord blood stem cells, progenitor cells, bone marrow stem cells, induced pluripotent stem cells, totipotent stem cells, or hematopoietic stem cells.

[0146] In some embodiments, the cells are obtained or prepared from peripheral blood. In some embodiments, the cells are obtained or prepared from peripheral blood mononuclear cells (PBMCs). In some embodiments, the cells are obtained or prepared from bone marrow. In some embodiments, the cells are obtained or prepared from umbilical cord blood. In some embodiments, the cells are human cells. In some embodiments, the cells are transfected or transduced with a nucleic acid vector using a method selected from the group consisting of electroporation, sonoporation, biolistics (e.g., Gene Gun), lipid transfection, polymer transfection, nanoparticles, viral transfection (e.g., retrovirus, lentivirus, AAV) or polyplex.

[0147] In some embodiments, engineered immune cells expressing an antigen-specific CAR of the present disclosure on their cell surface membrane comprise a percentage of stem cell memory and central memory cells that is greater than 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100%.

[0148] In some embodiments, engineered immune cells expressing an antigen-specific CAR of the present disclosure on their cell surface membrane are between about 10% to about 100%, between about 10% to about 90%, between about 10% to about 80%, between about 10% to about 70%, between about 10% to about 60%, between about 10% to about 50%, between about 10% to about 40%, between about 10% to about 30%, between about 10% to about 20%, between about 15% to about 100%, between about 15% to about 90% , about 15% to about 80%, about 15% to about 70%, about 15% to about 60%, about 15% to about 50%, about 15% to about 40%, about 15% to about 30%, about 20% to about 100%, about 20% to about 90%, about 20% to about 80%, about 20% to about 70%, about 20% to about 60%, about 20% to about 50%, about 20% to about 40%, about 20% to about 30%, about 30% to about 100%, about 30% to about 90%, about 30% to about 80%, about 30% to about 70%, about 30% to about 60%, about 30% to about 50%, about 30% to about 40%, about 40% to about 100%, about 40% to about 90%, about 40% to about 80%, about 40% to about 70%, about 40% to about 60%, about 40% to about 50%, about 50% to about 100%, about 50% to about 90%, about 50% to about 80%, about 50% to about 70%, about 50 % to about 60%, about 60% to about 100%, about 60% to about 90%, about 60% to about 80%, about 60% to about 70%, about 70% to about 90%, about 70% to about 80%, about 80% to about 100%, about 80% to about 90%, about 90% to about 100%, about 25% to about 50%, about 75% to about 100%, or about 50% to about 75% of stem cell memory and central memory cells.

[0149] In some embodiments, engineered immune cells expressing an antigen-specific CAR of the present disclosure on their cell surface membrane comprise a percentage of stem cell memory and central memory cells that is greater than 10%, 20%, 30%, 40%, 50%, or 60%.

[0150] In some embodiments, engineered immune cells expressing an antigen-specific CAR of the present disclosure on their cell surface membrane comprise a percentage of stem cell memory and central memory cells of about 10% to about 60%, about 10% to about 50%, about 10% to about 40%, about 15% to about 50%, about 15% to about 40%, about 20% to about 60%, or about 20% to about 70%.

[0151] In some embodiments, engineered immune cells expressing an antigen-specific CAR of the present disclosure on their cell surface membrane are CM Cells and / or T SCM The engineered immune cells are enriched for T cells, such that the engineered immune cells are enriched for T cells that are at least about 60%, 65%, 70%, 75%, or 80% bound. CM and T SCM In some embodiments, the engineered immune cells expressing the antigen-specific CAR of the present disclosure on their cell surface membrane include T CM Cells and / or T SCM The engineered immune cells are enriched for T cells, whereby the engineered immune cells are enriched for T cells that are at least about 70% of the bound T cells. CM and T SCM In some embodiments, the engineered immune cells expressing the antigen-specific CAR of the present disclosure on their cell surface membrane include T CM Cells and / or T SCM The engineered immune cells are enriched for T cells, such that the engineered immune cells are enriched for T cells that are at least about 75% of the bound T cells. CM and / or T SCM Contains cells.

[0152] In some embodiments, engineered immune cells expressing an antigen-specific CAR of the present disclosure on their cell surface membrane are CM Cells and / or T SCM The engineered immune cells are enriched for T cells, whereby the engineered immune cells are enriched for T cells that are at least 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, or 60% bound. SCM In some embodiments, the engineered immune cells expressing the antigen-specific CAR of the present disclosure on their cell surface membrane include T CM Cells and / or T SCMcells, whereby the engineered immune cells are enriched for at least 30%, 35%, 40%, 45%, or more of the bound T SCM Contains cells.

[0153] In some embodiments, the immune cell is an inflammatory T lymphocyte expressing any one of the CARs described herein. In some embodiments, the immune cell is a cytotoxic T lymphocyte expressing any one of the CARs described herein. In some embodiments, the immune cell is a regulatory T lymphocyte expressing any one of the CARs described herein. In some embodiments, the immune cell is a helper T lymphocyte expressing any one of the CARs described herein.

[0154] Also provided herein is a cell line obtained from a transformed immune cell (e.g., T cell) according to any of the above-mentioned methods. Also provided herein is a modified cell resistant to immunosuppressive treatment. In some embodiments, the isolated cell according to the present disclosure comprises a polynucleotide encoding a CAR.

[0155] In some embodiments, the engineered immune cells according to the present disclosure may contain one or more disrupted or inactivated genes. In some embodiments, the engineered immune cells according to the present disclosure contain one disrupted or inactivated gene selected from the group consisting of CD52, DLL3, GR, PD-1, CTLA-4, LAG3, TIM3, BTLA, BY55, TIGIT, B7H5, LAIR1, SIGLEC10, 2B4, HLA, TCRα, and TCRβ, and / or express a CAR, a multi-chain CAR, and / or a pTα transgene. In some embodiments, the isolated cells contain a polynucleotide encoding a polypeptide comprising a multi-chain CAR. In some embodiments, the isolated cells according to the present disclosure comprise two disrupted or inactivated genes selected from the group consisting of: CD52 and GR, CD52 and TCRα, CDR52 and TCRβ, DLL3 and CD52, DLL3 and TCRα, DLL3 and TCRβ, GR and TCRα, GR and TCRβ, TCRα and TCRβ, PD-1 and TCRα, PD-1 and TCRβ, CTLA-4 and TCRα, CTLA-4 and TCRβ, LAG3 and TCRα, LAG3 and TCRβ, TIM3 and Expressing TCRα, Tim3 and TCRβ, BTLA and TCRα, BTLA and TCRβ, BY55 and TCRα, BY55 and TCRβ, TIGIT and TCRα, TIGIT and TCRβ, B7H5 and TCRα, B7H5 and TCRβ, LAIR1 and TCRα, LAIR1 and TCRβ, SIGLEC10 and TCRα, SIGLEC10 and TCRβ, 2B4 and TCRα, 2B4 and TCRβ and / or CAR, multi-chain CAR and pTα transgenes. In some embodiments, the method includes disrupting or inactivating one or more genes by introducing an endonuclease into the cell, which can selectively inactivate the gene by selective DNA cleavage.In some embodiments, the endonuclease may be, for example, a zinc finger nuclease (ZFN), a megaTAL nuclease, a meganuclease, a transcription activator-like effector nuclease (TALE nuclease), or a CRIPR (e.g., Cas9) endonuclease.

[0156] In some embodiments, the TCR is not functional in the cells according to the present disclosure by disrupting or inactivating the TCR alpha and / or TCR beta genes. In some embodiments, a method of obtaining modified cells from an individual is provided, wherein the cells are capable of growing independently of the major histocompatibility complex (MHC) signaling pathway. Modified cells capable of growing independently of the MHC signaling pathway are amenable to being obtained by the present method and are therefore encompassed within the scope of the present disclosure. The modified cells disclosed herein can be used to treat a patient in need thereof against host-versus-graft (HvG) rejection and graft-versus-host disease (GvHD), and thus, within the scope of the present disclosure, a method of treating a patient in need thereof against host-versus-graft (HvG) rejection and graft-versus-host disease (GvHD) comprises treating said patient by administering to said patient an effective amount of modified cells comprising a disrupted or inactivated TCR alpha and / or TCR beta gene.

[0157] In some embodiments, the immune cells are engineered to be resistant to one or more chemotherapeutic agents. The chemotherapeutic agents may be, for example, purine nucleotide analogs (PNAs), thus making the immune cells suitable for cancer treatment combining adoptive immunotherapy and chemotherapy. Exemplary PNAs include, for example, clofarabine, fludarabine, cyclophosphamide, and cytarabine, alone or in combination. PNAs are metabolized by deoxycytidine kinase (dCK) to monophosphate PNAs, diphosphate PNAs, and triphosphate PNAs. Their triphosphate forms compete with ATP for DNA synthesis, act as proapoptotic agents, and are potent inhibitors of ribonucleotide reductase (RNR), which is involved in trinucleotide production.

[0158] In some embodiments, the isolated cells or cell lines of the present disclosure may comprise pTα or a functional variant thereof. In some embodiments, the isolated cells or cell lines may be further genetically modified by disrupting or inactivating the TCRα gene.

[0159] The present disclosure also provides engineered immune cells comprising any of the CAR polynucleotides described herein. In some embodiments, the CAR can be introduced into immune cells as a transgene via a plasmid vector. In some embodiments, the plasmid vector can also contain a selection marker, for example, to provide identification and / or selection of cells that have received the vector.

[0160] The CAR polypeptide may be synthesized in situ within the cell after introduction of a polynucleotide encoding the CAR polypeptide into the cell. Alternatively, the CAR polypeptide may be produced outside the cell and then introduced into the cell. Methods for introducing a polynucleotide construct into a cell are known in the art. In some embodiments, stable transformation methods (e.g., using lentiviral vectors) may be used to integrate the polynucleotide construct into the genome of the cell. In other embodiments, transient transformation methods may be used to transiently express the polynucleotide construct, and polynucleotide constructs that are not integrated into the genome of the cell. In other embodiments, viral-mediated methods may be used. The polynucleotide may be introduced into the cell by any suitable means, such as, for example, recombinant viral vectors (e.g., retroviruses, adenoviruses), liposomes, etc. Transient transformation methods include, for example, but are not limited to, microinjection, electroporation, or particle bombardment. The polynucleotide may be included in a vector, such as, for example, a plasmid vector or a viral vector.

[0161] In some embodiments, an isolated nucleic acid is provided that includes a promoter operably linked to a first polynucleotide encoding an antigen binding domain, at least one costimulatory molecule, and an activation domain. In some embodiments, the nucleic acid construct is contained within a viral vector. In some embodiments, the viral vector is selected from the group consisting of a retroviral vector, a murine leukemia virus vector, a SFG vector, an adenoviral vector, a lentiviral vector, an adeno-associated virus (AAV) vector, a herpes virus vector, and a vaccinia virus vector. In some embodiments, the nucleic acid is contained within a plasmid.

[0162] 3. Cell Growth Medium for In Vitro Expansion of Immune Cells In vitro expansion of cells (including expansion of immune cells such as genetically modified T cells) a first stimulator of cell proliferation (e.g., a first cytokine that stimulates T cell proliferation); a second stimulator of cell proliferation (e.g., a second cytokine that stimulates T cell proliferation); This can be achieved using cell growth medium that contains extracellular regulators of cell metabolism (e.g., T cell metabolism).

[0163] The immune cells of the present disclosure can be activated and expanded using methods generally known and generally described herein, either before or after genetic modification of the immune cells. In some embodiments, the immune cells (e.g., T cells) are activated and expanded before genetic modification of the immune cells. In some embodiments, the immune cells (e.g., T cells) are activated and expanded after genetic modification of the immune cells (e.g., engineered immune cells, including those described herein).

[0164] Thus, suitable conditions for T cell proliferation include a suitable medium (e.g., basal medium or RPMI medium 1640 or X-vivo 15, (Lonza)) containing primary and secondary stimulants of cell proliferation (e.g., IL-7 and IL-15), and extracellular regulators of cell metabolism (e.g., extracellular potassium), and may further include additional factors necessary for proliferation and viability, including serum (e.g., fetal bovine or human serum), IL-2, insulin, IFN-γ, IL-4, IL-7, GM-CSF, IL-10, IL-2, IL-15, TGF beta, and TNF, or any other additives for proliferation of cells known to those of skill in the art. In some embodiments, the medium for T cell culture does not contain exogenous IL-2. Other additives for proliferation of cells include, but are not limited to, detergents, plasmanate, and reducing agents such as N-acetyl-cysteine ​​and 2-mercaptoethanol. The medium can be RPMI 1640, A1M-V, DMEM, MEM, a-MEM, F-12, X-Vivo15, and X-Vivo20, Optimizer negative with additional amino acids, sodium pyruvate, and vitamins, serum-free or supplemented with an appropriate amount of serum (or plasma) or a defined set of hormones and / or cytokines in sufficient amounts for proliferation of T cells (e.g., IL-7 and / or IL-15). Antibiotics, such as penicillin and streptomycin, are included only in experimental cultures and not in cultures of cells injected into subjects. Target cells are maintained under conditions necessary to support proliferation, such as, for example, an appropriate temperature (e.g., 37°C) and atmosphere (e.g., air + 5% CO2).

[0165] T cells exposed to various stimulation times can exhibit different characteristics.

[0166] The method described herein can further include contacting the cells with an agent that stimulates the CD3 TCR complex and costimulatory molecules on the surface of the T cells to generate an activation signal for the T cells. For example, a chemical such as calcium ionophore A23187, phorbol 12-myristate 13-acetate (PMA), or a mitogenic lectin such as phytohemagglutinin (PHA) can be used to generate an activation signal for the T cells.

[0167] In some embodiments, the T cell population can also be stimulated in vitro, for example, by contacting with an anti-CD3 antibody or an antigen-binding fragment thereof, or an anti-CD2 antibody immobilized on a surface, or by contacting with a protein kinase C activator (e.g., bryostatin) in combination with a calcium ionophore. Co-stimulation of accessory molecules on the surface of T cells uses a ligand that binds to the accessory molecule. For example, a population of T cells can be contacted with an anti-CD3 antibody and an anti-CD28 antibody under conditions appropriate to stimulate proliferation of the T cells. The anti-CD3 antibody and the anti-CD28 antibody can be disposed on a bead or plate or other substrate.

[0168] In some embodiments, the cells of the present disclosure can be expanded by co-culturing with tissue or cells. The cells can also be expanded in vivo, for example, in the blood of a subject after administration of the cells to the subject.

[0169] In particular, the cell growth media and methods of use thereof may be particularly useful for the production of T cells, including CAR-T cells, such as allogeneic CAR-T cells.

[0170] A stimulant of cell proliferation In some embodiments, the first and second stimulating agents are selected from an agent that stimulates the CD3 TCR complex, an anti-CD3 antibody or antigen-binding fragment thereof, an anti-CD2 antibody or antigen-binding fragment thereof, a protein kinase C activator, or a growth factor (e.g., T cell growth factor), or any combination thereof.

[0171] In some embodiments, the stimulator of immune cells (e.g., T cells or engineered immune cells) is an agent that stimulates the CD3 TCR complex and costimulatory molecules on the surface of T cells to generate an activation signal for T cells. For example, chemicals such as calcium ionophore A23187, phorbol 12-myristate 13-acetate (PMA), or mitogenic lectins such as phytohemagglutinin (PHA) can be used to generate an activation signal for T cells.

[0172] In some embodiments, the stimulator of immune cells (e.g., T cells or engineered immune cells) is an anti-CD3 antibody, or an antigen-binding fragment thereof, or an anti-CD2 antibody immobilized on a surface, or a protein kinase C activator (e.g., bryostatin) in conjunction with a calcium ionophore. Co-stimulation of accessory molecules on the surface of T cells uses a ligand that binds to the accessory molecule. For example, a population of T cells can be contacted with anti-CD3 and anti-CD28 antibodies under conditions appropriate to stimulate proliferation of the T cells. The anti-CD3 and anti-CD28 antibodies can be disposed on beads or plates or other substrates.

[0173] In some embodiments, the stimulatory agent for the cells is a growth factor. In some embodiments, the stimulatory agent for the cells is a T cell growth factor. In some embodiments, the T cell growth factor is a cytokine. In some embodiments, the first stimulatory agent is a first cytokine and the second stimulatory agent is a second cytokine. In some embodiments, the cytokine is an interleukin (e.g., IL-2, IL-4, IL-7, IL-10, IL-12, or IL-15). In some embodiments, the first stimulatory agent and the second stimulatory agent are selected from the group consisting of IL-4, IL-7, IL-10, IL-12, and IL-15.

[0174] In some embodiments, the first stimulating agent is IL-7 and the second stimulating agent is IL-15.

[0175] In some embodiments, the cell growth medium excludes IL-2 (e.g., the cell growth medium excludes exogenous IL-2, and IL-2 is not present in the cell growth medium as a first stimulating agent and / or a second stimulating agent).

[0176] In some embodiments, the amounts of the first and second stimulating agents used are described in terms of concentration (e.g., using IU / mL). Thus, a "concentration ratio of x:y" can be used to describe the ratio of the concentration of the first stimulating agent (e.g., xIU / mL of IL-7) to the concentration of the second stimulating agent (e.g., yIU / mL of IL-15).

[0177] In some embodiments, the first stimulating agent (e.g., a first cytokine such as IL-7) and the second stimulating agent (e.g., a second cytokine such as IL-15) are present in a concentration ratio of about 10,000:1 to about 1:10,000, about 1,000:1 to about 1:1000, about 100:1 to about 1:100, or about 10:1 to about 1:10.

[0178] In some embodiments, the first stimulatory agent (e.g., a first cytokine such as IL-7) is present in a greater amount than the second stimulatory agent (e.g., a second cytokine such as IL-15). In some embodiments, the first stimulatory agent (e.g., a first cytokine such as IL-7) and the second stimulatory agent (e.g., a second cytokine such as IL-15) are present in a concentration ratio of about 1000:1 to about 10:1, about 900:1 to about 10:1, about 800:1 to about 10:1, about 700:1 to about 10:1, about 600:1 to about 10:1, about 500:1 to about 10:1, about 400:1 to about 10:1, about 300:1 to about 10:1, about 250:1 to about 10:1, about 200:1 to about 10:1, about 150:1 to about 10:1, or about 100:1 to about 4:1. In some embodiments, the first stimulatory agent (e.g., a first cytokine such as IL-7) and the second stimulatory agent (e.g., a second cytokine such as IL-15) are present in a concentration ratio of about 1000:1 to about 50:1, about 900:1 to about 50:1, about 800:1 to about 50:1, about 700:1 to about 50:1, about 600:1 to about 50:1, about 500:1 to about 50:1, about 400:1 to about 50:1, about 300:1 to about 50:1, about 250:1 to about 50:1, about 200:1 to about 50:1, about 150:1 to about 50:1, or about 100:1 to about 50:1. In some embodiments, the first stimulatory agent (e.g., a first cytokine such as IL-7) and the second stimulatory agent (e.g., a second cytokine such as IL-15) are present in a concentration ratio of about 200:1 to about 10:1, about 150:1 to about 10:1, about 125:1 to about 10:1, or about 100:1 to about 4:1. In some embodiments, the first stimulatory agent (e.g., a first cytokine such as IL-7) and the second stimulatory agent (e.g., a second cytokine such as IL-15) are present in a concentration ratio of about 100:1. In some embodiments, the first stimulatory agent (e.g., a first cytokine such as IL-7) and the second stimulatory agent (e.g., a second cytokine such as IL-15) are present in a concentration ratio of about 5:1, about 6:1, or about 7.1. In some embodiments, the first stimulating agent (eg, a first cytokine such as IL-7) and the second stimulating agent (eg, a second cytokine such as IL-15) are present in a concentration ratio of 6.25:1.

[0179] In some embodiments, the first stimulatory agent (e.g., a first cytokine such as IL-7) is present at a concentration of about 100 IU / mL to about 20,000 IU / mL. In some embodiments, the second stimulatory agent (e.g., a second cytokine such as IL-15) is present at a concentration of about 1 IU / mL to about 200 IU / mL.

[0180] In some embodiments, the first stimulatory agent (e.g., a first cytokine such as IL-7) is present at a concentration of about 100 IU / mL to about 10,000 IU / mL. In some embodiments, the second stimulatory agent (e.g., a second cytokine such as IL-15) is present at a concentration of about 1 IU / mL to about 100 IU / mL.

[0181] In some embodiments, the first stimulating agent (e.g., a first cytokine such as IL-7) is present at a concentration of about 100 IU / mL to about 1,000 IU / mL, about 300 IU / mL to about 5,000 IU / mL, or about 3,000 IU / mL. In some embodiments, the first stimulating agent (e.g., a first cytokine such as IL-7) is present at a concentration of about 3,000 IU / mL to about 5,000 IU / mL.

[0182] In some embodiments, the first stimulatory agent (e.g., a first cytokine such as IL-7) is present at a concentration of about 2500 IU / mL to about 7500 IU / mL. In some embodiments, the second stimulatory agent (e.g., a second cytokine such as IL-15) is present at a concentration of about 25 IU / mL to about 75 IU / mL.

[0183] In some embodiments, the first stimulatory agent (e.g., a first cytokine such as IL-7) is present at a concentration of about 4000 IU / mL to about 6000 IU / mL. In some embodiments, the second stimulatory agent (e.g., a second cytokine such as IL-15) is present at a concentration of about 40 IU / mL to about 60 IU / mL. In some embodiments, the second stimulatory agent (e.g., a second cytokine such as IL-15) is present at a concentration of about 25 IU / mL to about 100 IU / mL or about 50 IU / mL.

[0184] In some embodiments, the first stimulating agent (e.g., a first cytokine such as IL-7) is present at a concentration of about 5000 IU / mL. In some embodiments, the second stimulating agent (e.g., a second cytokine such as IL-15) is present at a concentration of about 50 IU / mL.

[0185] In some embodiments, the immune cells (e.g., T cells, such as genetically modified T cells) are contacted with a first stimulatory agent (e.g., a first cytokine, such as IL-7) and a second stimulatory agent (e.g., a second cytokine, such as IL-15) about every 1, 2, 3, 4, 5, 6, 7, or 8 days. In some embodiments, the immune cells (e.g., T cells, such as genetically modified T cells) are contacted with a first stimulatory agent (e.g., a first cytokine, such as IL-7) and a second stimulatory agent (e.g., a second cytokine, such as IL-15) about every week. In some embodiments, the immune cells (e.g., T cells, such as genetically modified T cells) are contacted with a first stimulatory agent (e.g., a first cytokine, such as IL-7) and a second stimulatory agent (e.g., a second cytokine, such as IL-15) 1, 2, 3, 4, 5, 6, 7, or 8 times during an expansion process lasting about 1, 2, 3, 4, 5, 6, 7 days (e.g., about 1 week), about 2 weeks, about 3 weeks, or about 4 weeks. In some embodiments, the immune cells (e.g., T cells, such as genetically modified T cells) are contacted with a first stimulatory agent (e.g., a first cytokine, such as IL-7) and a second stimulatory agent (e.g., a second cytokine, such as IL-15) for about 2 weeks.

[0186] B extracellular regulators of cellular metabolism In some embodiments, the cell growth medium further comprises an extracellular regulator of cell metabolism in addition to the first stimulatory agent (e.g., a first cytokine such as IL-7) and the second stimulatory agent (e.g., a second cytokine such as IL-15) described herein.

[0187] In some embodiments, the extracellular regulator is extracellular potassium (K + In some embodiments, extracellular potassium is present at a concentration of about 4 mM to about 50 mM, about 4 mM to about 40 mM, about 4 mM to about 30 mM, about 10 mM to about 40 mM, about 10 mM to about 30 mM, about 15 mM to about 40 mM, about 15 mM to about 35 mM, about 15 mM to about 30 mM, or about 20 mM to about 30 mM. In some embodiments, extracellular potassium is present at a concentration of about 10 mM to about 30 mM, or about 20 mM to about 30 mM.

[0188] In some embodiments, extracellular potassium is present at a concentration of about 8 mM, 9 mM, 10 mM, 11 mM, 12 mM, 13 mM, 14 mM, 15 mM, 16 mM, 17 mM, 18 mM, 19 mM, 20 mM, 21 mM, 22 mM, 23 mM, 24 mM, 25 mM, 26 mM, 27 mM, 28 mM, 29 mM, or 30 mM.

[0189] In some embodiments, extracellular potassium is present at a concentration greater than about 15 mM. In some embodiments, extracellular potassium is present at a concentration of about 20 mM or greater. In some embodiments, extracellular potassium is present at a concentration of about 20 mM, 21 mM, 22 mM, 23 mM, 24 mM, 25 mM, 26 mM, 27 mM, 28 mM, 29 mM, or 30 mM.

[0190] In some embodiments, the extracellular potassium is present at a concentration of about 10 mM or greater. In some embodiments, the extracellular potassium is present at a concentration of about 20 mM or greater. In some embodiments, the extracellular potassium is present at a concentration of about 30 mM or less. In some embodiments, the extracellular potassium is present at a concentration of about 25 mM.

[0191] In some embodiments, extracellular potassium is provided as KCl.

[0192] c. Additional Features and Components of Cell Growth Media The cell growth medium described herein (e.g., a cell growth medium comprising a first stimulant that is IL-7, a second stimulant that is IL-15, and an extracellular regulatory factor that is extracellular potassium) can be used in a variety of methods of activating and expanding T cells that are known in the art and described, for example, in U.S. Pat. No. 6,905,874, U.S. Pat. No. 6,867,041, U.S. Pat. No. 6,797,514, and PCT WO2012 / 079000, the contents of which are incorporated herein by reference in their entireties.

[0193] In some embodiments, the method further comprises contacting the PBMCs or isolated T cells with culture medium having appropriate cytokines, such as IL-2, and additional stimulatory and costimulatory molecules, such as anti-CD3 and anti-CD28 antibodies, typically attached to beads or other surfaces. The anti-CD3 and anti-CD28 antibodies bound to the beads serve as "surrogate" antigen-presenting cells (APCs). One example is the Dynabeads® system, which is a CD3 / CD28 activator / stimulator for physiological activation of human T cells. In other embodiments, the T cells can be activated and stimulated to grow with feeder cells and appropriate antibodies and further other cytokines (e.g., in addition to IL-7 and IL-15) using methods described in U.S. Pat. No. 6,040,177, U.S. Pat. No. 5,827,642, and WO2012129514, the contents of which are incorporated herein by reference in their entirety. In some embodiments, the cell growth medium and methods of use thereof exclude exogenous IL-2 as a T cell growth factor.

[0194] Other conditions suitable for T cell culture include, for example, a suitable medium (e.g., basal medium or RPMI medium 1640 or X-vivo™ medium, (Lonza)) that can contain primary and secondary stimuli for proliferation and viability for cell growth and can contain additional factors such as serum (fetal bovine or human serum), insulin, IFN-γ, GM-CSF, TGFβ, and TNF, or any additives for the growth of cells known to those skilled in the art. Other additives for the growth of cells include, but are not limited to, detergents, plasmanate, and reducing agents such as N-acetyl-cysteine ​​and 2-mercaptoethanol. The medium may include RPMI 1640, A1M-V, DMEM, MEM, a-MEM, F-12, X-Vivo™ 10, X-Vivo™ 15 and X-Vivo™ 20, OpTmizer™, may contain additional amino acids, sodium pyruvate, and / or vitamins, and may be serum-free or supplemented with an appropriate amount of serum (or plasma) and / or a defined set of hormones. Antibiotics, such as penicillin and streptomycin, are included only in the experimental cultures and not in the cultures of cells injected into the subject. Target cells are maintained under conditions necessary to support growth, such as, for example, an appropriate temperature (e.g., 37°C) and atmosphere (e.g., air + 5% CO2). T cells exposed to various stimulation times may exhibit different characteristics.

[0195] In some embodiments, the cells of the present disclosure can be expanded by co-culturing with tissue or cells. The cells can also be expanded in vivo, for example, in the blood of a subject after administration of the cells to the subject.

[0196] In some embodiments, the cell growth medium contains serum. In some embodiments, the cell growth medium is serum-free. In some embodiments, the cell growth medium is xenobiotic-free. In some embodiments, the cell growth medium is chemically defined.

[0197] D. Cell proliferation In some embodiments, culturing of cells (including immune cells such as T cells) for in vitro expansion using a cell growth medium described herein (e.g., a cell growth medium comprising a first stimulant that is IL-7, a second stimulant that is IL-15, and an extracellular regulatory factor that is extracellular potassium), or any method described herein, continues for about 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days (e.g., about 1 week), about 2 weeks, about 3 weeks, or about 4 weeks. In some embodiments, the period is about 1 week to about 3 weeks or about 1 week to about 2 weeks (e.g., about 7 to 15 days or about 10 to 14 days).

[0198] In some embodiments, a cell growth medium described herein (e.g., a cell growth medium comprising a first stimulant that is IL-7, a second stimulant that is IL-15, and an extracellular regulator that is extracellular potassium) or any of the methods described herein results in cell growth that is about 10 to about 10,000 fold when measured over about 1 week to about 3 weeks. In some embodiments, about 100-fold to about 1,000-fold cell growth is observed over about 7 to 15 days (e.g., about 10 to 14 days or about 2 weeks). In some embodiments, at least about 100-fold cell growth is observed over about 7 to 15 days (e.g., about 10 to 14 days or about 2 weeks). In some embodiments, at least about 200-fold cell growth is observed over about 7 to 15 days (e.g., about 10 to 14 days or about 2 weeks).

[0199] In some embodiments, any of the cell growth media (e.g., cell growth media comprising a first stimulant that is IL-7, a second stimulant that is IL-15, and an extracellular regulatory factor that is extracellular potassium) or methods of cell growth described herein can grow at least about 50×10 cells / mL for about 8-16 days. 6 ~About 100×10 6 In some embodiments, the cell number is at least about 100×10 over a period of about 10-14 days. 6 (e.g., at least about 100×10 over a period of about 10-14 days) 6 , about 125×10 6, about 150×10 6 , or about 100 × 10 6 ).

[0200] e.Cell phenotype In some embodiments, a cell growth medium described herein (e.g., a cell growth medium comprising a first stimulant that is IL-7, a second stimulant that is IL-15, and an extracellular regulatory factor that is extracellular potassium), or any method of use thereof, produces a desired phenotype for the production of engineered immune cells.

[0201] In some embodiments, a T cell expansion medium (e.g., a T cell expansion medium comprising a first stimulatory agent that is IL-7, a second stimulatory agent that is IL-15, and an extracellular regulatory factor that is extracellular potassium) described herein, or any method of use thereof, is CM and T SCM A cell-enriched population of T cells (e.g., genetically modified T cells) is produced.

[0202] In some embodiments, T CM Cells and / or T SCM The enriched population of T cells (e.g., genetically modified T cells) may be at least about 60%, 65%, 70%, 75%, or 80% T CM and T SCM In some embodiments, the T CM Cells and / or T SCM The enriched T cell population is comprised of at least about 70% T CM and T SCM In some embodiments, the T CM Cells and / or T SCM The enriched population of T cells (e.g., genetically modified T cells) comprises at least about 75% T CM and T SCM Includes binding cells with.

[0203] In some embodiments, T CM Cells and / or T SCMThe enriched population of T cells (e.g., genetically modified T cells) may be at least about 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, or 60% T SCM In some embodiments, the T CM Cells and / or T SCM The enriched T cell population may be at least about 30%, 35%, 40%, or 45% T SCM Cells Includes cells.

[0204] 4. Manufacturing engineered immune cells (including CAR T cells) Provided herein are methods of using the cell growth media described herein for the manufacture of immune cells, including engineered immune cells such as CAR T cells.

[0205] Various known techniques can be used to produce polynucleotides, polypeptides, vectors, antigen-binding domains, immune cells, compositions, and the like according to the present disclosure.

[0206] Prior to in vitro manipulation or genetic modification of immune cells as described herein, the cells can be obtained from a subject. The cells expressing a CAR can be derived from an allogeneic or autologous process.

[0207] a. Raw materials The cell growth media described herein (e.g., containing IL-7+IL-15 and increased extracellular potassium) can be used to culture a variety of cells, including the in vitro expansion of various immune cells (e.g., genetically modified T cells). Exemplary cells are described herein.

[0208] In some embodiments, the immune cells include T cells. T cells can be obtained from a number of sources, including peripheral blood mononuclear cells (PBMCs), bone marrow, lymph node tissue, umbilical cord blood, thymus tissue, tissue from an infected site, ascites, pleural effusion, spleen tissue, and tumor. In some specific embodiments, T cells can be obtained from a volume of blood drawn from a subject using any number of techniques known to those skilled in the art, such as FICOLL™ separation.

[0209] Cells can be obtained from the circulating blood of an individual by apheresis. Apheresis products typically contain lymphocytes, including T cells, monocytes, granulocytes, B cells, other nucleated white blood cells, red blood cells, and platelets. In certain embodiments, the cells collected by apheresis can be washed to remove plasma fraction, and then placed in a suitable buffer or medium for subsequent processing.

[0210] In certain embodiments, T cells are isolated from PBMCs by lysing red blood cells and depleting monocytes, for example, using centrifugation through a PERCOLL™ gradient. Specific subpopulations of T cells (e.g., CD28+, CD4+, CD45RA-, and CD45RO+ T cells or CD28+, CD4+, CDS+, CD45RA-, CD45RO+, and CD62L+ T cells) can be further isolated by positive or negative selection techniques known in the art. For example, enrichment of T cell populations by negative selection can be achieved using a combination of antibodies directed against surface markers unique to the negatively selected cells. One method for use herein is cell sorting and / or selection via negative magnetic immunoadherence or flow cytometry using a cocktail of monoclonal antibodies directed against cell surface markers present on the negatively selected cells. For example, to enrich for CD4+ cells by negative selection, a monoclonal antibody cocktail typically includes antibodies to CD14, CD20, CD11b, CD16, HLA-DR, and CD8. Flow cytometry and cell sorting can also be used to isolate cell populations of interest for use in the present disclosure.

[0211] PBMCs can be used directly for genetic modification with immune cells (such as CAR or TCR) using the methods described herein. In certain embodiments, after isolating PBMCs, T lymphocytes can be further isolated and both cytotoxic and helper T lymphocytes can be sorted into naive, memory, and effector T cell subpopulations before or after genetic modification and / or expansion.

[0212] In some embodiments, CD8+ cells are further sorted into naive, stem cell memory, central memory, and effector cells by identifying cell surface antigens associated with each of these types of CD8+ cells. In some embodiments, the expression of phenotypic markers of central memory T cells includes CD27, CD45RA, CD45RO, CD62L, CCR7, CD28, CD3, and CD127, and is negative for granzyme B. In some embodiments, stem cell memory T cells are CD45RO-, CD62L+, CD8+ T cells. In some embodiments, central memory T cells are CD45RO+, CD62L+, CD8+ T cells. In some embodiments, effector T cells are negative for CD62L, CCR7, CD28, and CD127, and positive for granzyme B and perforin. In certain embodiments, CD4+ T cells are further classified into subpopulations. For example, CD4+ T helper cells can be classified into naive, central memory, and effector cells by identifying cell populations that bear cell surface antigens.

[0213] b. Stem cell-derived immune cells In some embodiments, immune cells can be derived from embryonic stem (ES) cells or induced pluripotent stem (iPS) cells.Suitable HSC, mesenchymal, iPS cells, and other types of stem cells can be cultured immortal cell lines or directly isolated from patients.Various methods for isolating, expressing, and / or culturing stem cells are known in the art and can be used in the practice of the present disclosure.

[0214] In some embodiments, the immune cells are induced pluripotent stem cells (iPSCs) derived from reprogrammed T cells. In some embodiments, the source material may be induced pluripotent stem cells (iPSCs) derived from T cells or non-T cells. The source material may be embryonic stem cells. The source material may be B cells, or peripheral blood mononuclear cell isolates, hematopoietic progenitor cells, hematopoietic stem cells, mesenchymal stem cells, adipose stem cells, or any other cells of any other somatic cell type.

[0215] c. Genetic modification of isolated cells Immune cells such as T cells can be genetically modified after isolation using known methods, or immune cells can be activated and expanded (or differentiated in the case of progenitor cells) in vitro before being genetically modified. In some embodiments, isolated immune cells are genetically modified to reduce or eliminate expression of endogenous TCRα and / or CD52. In some embodiments, cells are genetically modified using gene editing techniques (e.g., CRISPR / Cas9, zinc finger nucleases (ZFNs), TALENs, MegaTALs, meganucleases) to reduce or eliminate expression of endogenous proteins (e.g., TCRα and / or CD52). In another embodiment, immune cells such as T cells are genetically modified with a chimeric antigen receptor (e.g., transduced with a viral vector comprising one or more nucleotide sequences encoding a CAR) as described herein, and then activated and / or expanded in vitro.

[0216] Certain methods for making the constructs and engineered immune cells of the present disclosure are described in PCT application PCT / US15 / 14520, the contents of which are incorporated herein by reference in their entirety.

[0217] It will be understood that PBMCs may further comprise other cytotoxic lymphocytes such as NK cells or NKT cells. An expression vector carrying the coding sequence of the chimeric receptor disclosed herein may be introduced into a population of human donor T cells, NK cells, or NKT cells. Successfully transduced T cells carrying the expression vector can be sorted using flow cytometry to isolate CD3 positive T cells, which can then be further expanded to increase the number of these CAR-expressing T cells in addition to cell activation using anti-CD3 antibodies and IL-2 or other methods known in the art as described elsewhere herein. Standard procedures are used for cryopreservation of CAR-expressing T cells for storage and / or preparation for use in human subjects. In one embodiment, in vitro transduction, culture, and / or expansion of T cells is performed in the absence of non-human animal derived products such as fetal calf serum and fetal bovine serum.

[0218] For cloning polynucleotides, vectors can be introduced into host cells (isolated host cells) to allow the vector to replicate itself, thereby amplifying the copies of the polynucleotides contained therein. Cloning vectors can generally include sequence components, including but not limited to origin of replication, promoter sequence, transcription initiation sequence, enhancer sequence, and selection marker. These elements can be appropriately selected by those skilled in the art. For example, origin of replication can be selected to promote autonomous replication of vectors in host cells.

[0219] In some particular embodiments, the present disclosure provides an isolated host cell that contains the vector provided herein.The host cell that contains the vector can be useful for expressing or cloning the polynucleotide contained in the vector.Suitable host cells can include, but are not limited to, prokaryotic cells, fungal cells, yeast cells, or higher eukaryotic cells such as mammalian cells, particularly human cells.

[0220] The vector can be introduced into the host cell using any suitable method known in the art, including, but not limited to, DEAE-dextran mediated delivery, calcium phosphate precipitation, cationic lipid mediated delivery, liposome mediated transfection, electroporation, microprojectile bombardment, receptor mediated gene delivery, polylysine, histone, chitosan, and peptide mediated delivery. Standard methods for transfection and transformation of cells for expression of vectors of interest are well known in the art. In a further embodiment, a mixture of different expression vectors can be used in genetically modifying a donor population of immune effector cells, each vector encoding a different CAR disclosed herein. The resulting transduced immune effector cells form a mixed population of engineered cells, with a proportion of the engineered cells expressing one or more different CARs.

[0221] In one embodiment, the present disclosure provides a method for preserving genetically engineered cells expressing CAR or TCR. This involves cryopreserving immune cells so that the cells remain viable when thawed. A fraction of immune cells expressing CAR can be cryopreserved by methods known in the art to provide a permanent source of such cells for future treatment of patients suffering from malignant tumors. When necessary, cryopreserved transformed immune cells can be thawed, grown, and expanded to obtain more such cells.

[0222] In some embodiments, the cells are formulated by first harvesting the cells from their culture medium, then washing and concentrating the cells in a medium and container system suitable for administration (a pharma- ceutical acceptable carrier) in a therapeutically effective amount. Suitable infusion media are any isotonic media formulation, typically saline, Normosol™ R (Abbott) or Plasma-Lyte™ A (Baxter), but 5% dextrose or Ringer's lactate in water can also be utilized. Infusion media can be supplemented with human serum albumin.

[0223] d. Allogeneic CAR T (ALLOCAR T(TM)) cells The process for manufacturing allogeneic CAR T therapy, or AlloCARs™, involves harvesting healthy, selected, screened, and tested T cells from healthy donors. The T cells are then engineered to express a CAR that recognizes a specific cell surface protein expressed on hematological or solid tumors. The allogeneic T cells are gene-edited to reduce the risk of graft-versus-host disease (GvHD) and prevent allogeneic rejection. The T cell receptor genes (e.g., TCRα, TCRβ) are knocked out to avoid GvHD. The CD52 gene can be knocked out to make the CAR T product resistant to anti-CD52 antibody treatment. Anti-CD52 antibody treatment can therefore be used to suppress the host immune system and allow the CAR T to remain engrafted and achieve full therapeutic effect. The engineered T cells then undergo a purification step and are cryopreserved in vials for eventual delivery to the patient.

[0224] e. Autologous CAR T (AUTOCAR T(TM)) cells Autologous chimeric antigen receptor (CAR) T cell therapy involves collecting a patient's own cells (e.g., white blood cells, including T cells) and genetically engineering the T cells to express a CAR that recognizes targets expressed on the cell surface of one or more specific cancer cells and kills the cancer cells. The engineered cells are then cryopreserved and subsequently administered to the patient.

[0225] 5. Pharmaceutical Compositions and Treatments In some embodiments, the cells are formulated by first harvesting the cells from their culture medium, then washing and concentrating the cells in a medium and container system suitable for administration (a pharma- ceutical acceptable carrier) in a therapeutically effective amount. Suitable infusion media are any isotonic media formulation, typically saline, Normosol™ R (Abbott) or Plasma-Lyte™ A (Baxter), but 5% dextrose or Ringer's lactate in water can also be utilized. Infusion media can be supplemented with human serum albumin.

[0226] In embodiments, the desired therapeutic amount of cells in the composition is generally at least 2 cells (e.g., at least one CD8+ central or stem cell memory T cell and at least one CD4+ helper T cell subset, or two or more CD8+ central or stem cell memory T cells, or two or more CD4+ helper T cell subsets), or more commonly, 10 2 More than 10 cells 6 Less than or equal to 10 7 , 10 8 or 10 9 Less than 10 cells 10 The number of cells will depend on the desired use for which the composition is intended, and the type of cells contained therein. Desired cell densities are typically between 10 6 cells / ml, generally greater than 10 7 More than 10 cells / ml 8 Clinically significant numbers of immune cells are >10 5 , 10 6 , 10 7 , 10 8 , 10 9 , 10 10 , 10 11 , or 10 12 The cells can be cumulatively distributed over multiple injections that are equal to or greater than about 10 cells per injection. In some embodiments of the present disclosure, particularly since all injected cells are redirected to a specific target antigen, 5 / kilogram or about 10 6 / kilogram (10 per patient) 6 ~10 11 ) can be administered. CAR therapy can be administered multiple times at doses within these ranges. The cells can be autologous, allogeneic, or xenogeneic to the patient undergoing therapy.

[0227] The CAR-expressing cell population of the present disclosure can be administered as a pharmaceutical composition alone or in combination with a diluent and / or in combination with other components such as IL-2 or other cytokines or cell populations. The pharmaceutical composition of the present disclosure can include a CAR- or TCR-expressing cell population, such as a T cell described herein, in combination with one or more pharma- ceutical or physiologically acceptable carriers, diluents, or excipients. Such compositions can include a buffer, such as neutral buffered saline, phosphate buffered saline, carbohydrates, such as glucose, mannose, sucrose, or dextran, amino acids, such as mannitol, proteins, polypeptides, or glycine, antioxidants, chelating agents, such as EDTA or glutathione, adjuvants (e.g., aluminum hydroxide), and preservatives. The compositions of the present disclosure are preferably formulated for intravenous administration.

[0228] Pharmaceutical compositions (solutions, suspensions, etc.) may contain one or more of the following: sterile diluents such as water for injection, saline, preferably saline, Ringer's solution, isotonic sodium chloride, fixed oils such as synthetic mono- or diglycerides that may serve as solvents or suspending media, polyethylene glycol, glycerin, propylene glycol or other solvents, antibacterial agents such as benzyl alcohol or methylparabens, antioxidants such as ascorbic acid or sodium sulfite, chelating agents such as ethylenediaminetetraacetic acid, buffers such as acetates, citrates or phosphates, agents for adjusting tonicity such as sodium chloride or dextrose. Parenteral preparations may be enclosed in ampoules, disposable syringes, or multiple dose vials made of glass or plastic. Pharmaceutical compositions for injection are preferably sterile.

[0229] 6.Treatment method The present disclosure includes a method for treating or preventing a disease (e.g., cancer) in a patient, comprising administering to a patient in need thereof an effective amount of at least one CAR, or immune cell comprising a CAR disclosed herein.

[0230] Methods for treating diseases or disorders, including cancer, are provided. In some embodiments, the disclosure relates to generating a T cell-mediated immune response in a subject, comprising administering to the subject an effective amount of the engineered immune cells of the present application. In some embodiments, the T cell-mediated immune response is directed against a target cell. In some embodiments, the engineered immune cells comprise a chimeric antigen receptor (CAR). In some embodiments, the target cell is a tumor cell. In some aspects, the disclosure includes a method for treating or preventing a malignancy, comprising administering to a subject in need thereof an effective amount of at least one isolated antigen binding domain described herein. In some aspects, the disclosure includes a method for treating or preventing a malignancy, comprising administering to a subject in need thereof an effective amount of at least one immune cell, wherein the immune cell comprises at least one chimeric antigen receptor, T cell receptor, and / or isolated antigen binding domain described herein. CARs containing the immune cells of the disclosure can be used to treat malignancies associated with aberrant expression of biomarkers. In some embodiments, CARs containing immune cells of the present disclosure can be used to treat small cell lung cancer, melanoma, low-grade glioma, glioblastoma, medullary thyroid carcinoma, carcinoid, dispersed neuroendocrine tumors in the pancreas, bladder and prostate, testicular cancer, and lung adenocarcinoma with neuroendocrine features. In an exemplary embodiment, CARs containing immune cells, such as CAR-T cells of the present disclosure, are used to treat small cell lung cancer.

[0231] Also provided is a method of reducing the size of a tumor in a subject, comprising administering to a subject an engineered cell of the present disclosure, wherein the cell comprises a chimeric antigen receptor that comprises an antigen binding domain and that binds to an antigen on the tumor.

[0232] In some embodiments, the subject has a solid tumor or a hematological malignancy such as lymphoma or leukemia. In some embodiments, the engineered cells are delivered to the tumor bed. In some embodiments, the cancer is present in the bone marrow of the subject. In some embodiments, the engineered cells are autologous immune cells, e.g., autologous T cells. In some embodiments, the engineered cells are allogeneic immune cells, e.g., allogeneic T cells. In some embodiments, the engineered cells are heterologous immune cells, e.g., heterologous T cells. In some embodiments, the engineered cells of the present application are transfected or transduced in vivo. In other embodiments, the engineered cells of the present application are transfected or transduced ex vivo. As used herein, the term "in vitro cells" refers to any cells that are cultured ex vivo.

[0233] A "therapeutically effective amount," "effective dose," "effective amount," or "therapeutically effective dose" of a therapeutic agent, e.g., engineered CART cells, is any amount that, when used alone or in combination with another therapeutic agent, protects a subject against onset of disease, or promotes disease regression as indicated by a decrease in the severity of disease symptoms, increases the frequency and duration of disease-free periods, or prevents injury or disability due to the disease. The ability of a therapeutic agent to promote disease regression can be evaluated using a variety of methods known to those of skill in the art, for example, by assaying the activity of the agent in human subjects during clinical trials, in animal model systems predictive of efficacy in humans, or in in vitro assays.

[0234] The terms "patient" and "subject" are used interchangeably and include human and non-human animal subjects, as well as those with a formally diagnosed disorder, those without a formally recognized disorder, those under medical attention, those at risk of developing a disorder, and the like.

[0235] The terms "treat" and "treatment" include therapeutic treatment, prophylactic treatment, and applications in which a subject reduces the risk of developing a disorder or other risk factors. Treatment does not require a complete cure of the disorder, but encompasses embodiments in which symptoms or underlying risk factors are reduced. The term "prevent" does not require 100% elimination of the likelihood of an event. Rather, it indicates that the likelihood of an event occurring in the presence of a compound or method is reduced.

[0236] A desired therapeutic amount of cells in a composition is generally at least 2 cells (e.g., at least one CD8+ central memory T cell and at least one CD4+ helper T cell subset), or more typically 10 2 Super cells, up to 10 6 pieces, maximum 10 8 cells or 10 9 Contains cells, 10 10 The number of cells depends on the desired use for which the composition is intended, and the type of cells contained therein. Desired cell densities are typically between 10 6 cells / ml, generally greater than 10 7 More than 10 cells / ml 8 Clinically significant numbers of immune cells are >10 5 , 10 6 , 10 7 , 10 8 , 10 9 , 10 10 , 10 11 , or 10 12 The cells can be cumulatively distributed over multiple injections equal to or greater than 10 cells per injection. In some aspects of the present disclosure, particularly since all injected cells are redirected to a specific target antigen, 6 / kilogram (10 per patient) 6 ~10 11 ) can be administered. CAR therapy can be administered multiple times at doses within these ranges. The cells can be autologous, allogeneic, or xenogeneic to the patient undergoing therapy.

[0237] In some embodiments, the therapeutically effective amount of CAR T cells is about 1×10 5 cells / kg, approx. 2X10 5 cells / kg, approx. 3X10 5 cells / kg, approx. 4X10 5 cells / kg, approx. 5X10 5 cells / kg, approximately 6X10 5 cells / kg, approximately 7X10 5 cells / kg, about 8X10 5 cells / kg, approx. 9X10 5 cells / kg, 2X10 6 cells / kg, approx. 3X10 6 cells / kg, approx. 4X10 6 cells / kg, approx. 5X10 6 cells / kg, approximately 6X10 6 cells / kg, approximately 7X10 6 cells / kg, about 8X10 6 cells / kg, approx. 9X10 6 cells / kg, approximately 1X10 7 cells / kg, approx. 2X10 7 cells / kg, approx. 3X10 7 cells / kg, approx. 4X10 7 cells / kg, approx. 5X10 7 cells / kg, approximately 6X10 7 cells / kg, approximately 7X10 7 cells / kg, about 8X10 7 cells / kg, or approximately 9X10 7 cells / kg.

[0238] In some embodiments, the target dose of CAR+ / CAR-T+ / TCR+ cells is 1×10 6 ~2×10 8 Cells / kg, e.g., 2 x 10 6 The range is 100 mg / kg of cells. Doses above and below this range may be appropriate for a particular subject, and appropriate dose levels can be determined by a medical professional, if necessary. Additionally, multiple doses of cells can be provided according to the present disclosure.

[0239] In some aspects, the disclosure includes a pharmaceutical composition comprising at least one antigen binding domain described herein and a pharma- ceutical acceptable excipient. In some embodiments, the pharmaceutical composition further comprises an additional active agent.

[0240] The CAR-expressing cell population of the present disclosure can be administered as a pharmaceutical composition alone or in combination with a diluent and / or in combination with other components such as IL-2 or other cytokines or cell populations. The pharmaceutical composition of the present disclosure can include a CAR- or TCR-expressing cell population, such as a T cell described herein, in combination with one or more pharma- ceutical or physiologically acceptable carriers, diluents, or excipients. Such compositions can include a buffer, such as neutral buffered saline, phosphate buffered saline, carbohydrates, such as glucose, mannose, sucrose, or dextran, amino acids, such as mannitol, proteins, polypeptides, or glycine, antioxidants, chelating agents, such as EDTA or glutathione, adjuvants (e.g., aluminum hydroxide), and preservatives. The compositions of the present disclosure are preferably formulated for intravenous administration.

[0241] Pharmaceutical compositions (solutions, suspensions, etc.) may contain one or more of the following: sterile diluents such as water for injection, saline, preferably saline, Ringer's solution, isotonic sodium chloride, fixed oils such as synthetic mono- or diglycerides that may serve as solvents or suspending media, polyethylene glycol, glycerin, propylene glycol or other solvents, antibacterial agents such as benzyl alcohol or methylparabens, antioxidants such as ascorbic acid or sodium sulfite, chelating agents such as ethylenediaminetetraacetic acid, buffers such as acetates, citrates or phosphates, agents for adjusting tonicity such as sodium chloride or dextrose. Parenteral preparations may be enclosed in ampoules, disposable syringes, or multiple dose vials made of glass or plastic. Pharmaceutical compositions for injection are preferably sterile.

[0242] In some embodiments, when administered to a patient, engineered immune cells expressing any one of the antigen-specific CARs described herein on their cell surface can reduce, kill, or lyse the patient's endogenous antigen-expressing cells. In one embodiment, the percentage of reduction or lysis of antigen-expressing endogenous cells or cells of a cell line expressing an antigen by engineered immune cells expressing any one of the antigen-specific CARs described herein is at least about 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or greater than 95%. In one embodiment, the percentage of reduction or lysis of antigen-expressing endogenous cells or cells of an antigen-expressing cell line by engineered immune cells expressing an antigen-specific CAR is about 5% to about 95%, about 10% to about 95%, about 10% to about 90%, about 10% to about 80%, about 10% to about 70%, about 10% to about 60%, about 10% to about 50%, about 10% to about 40%, about 20% to about 90%, about 20% to about 80%, about 20% to about 70%, about 20% to about 60%, about 20% to about 50%, about 25% to about 75%, or about 25% to about 60%. In one embodiment, the endogenous antigen-expressing cells are endogenous antigen-expressing bone marrow cells.

[0243] In one embodiment, the rate of reduction or lysis of target cells, e.g., cell lines expressing the antigen, by engineered immune cells expressing an antigen-specific CAR of the present disclosure on their cell surface membrane can be measured using the assays disclosed herein.

[0244] The method can further include administering one or more chemotherapeutic agents. In certain embodiments, the chemotherapeutic agent is a lymphodepleting (conditioning) chemotherapy. For example, a particular beneficial dose of cyclophosphamide (200 mg / m 2 / day~2000mg / m 2 / day, about 100mg / m 2 / day~about 2000mg / m 2 / day, e.g., about 100 mg / m 2 / day, about 200mg / m2 / day, about 300mg / m 2 / day, about 400mg / m 2 / day, about 500mg / m 2 / day, about 600mg / m 2 / day, about 700mg / m 2 / day, about 800mg / m 2 / day, approximately 900mg / m 2 / day, about 1000mg / m 2 / day, about 1500mg / m 2 / day or approximately 2000 mg / m 2 / day) and a specific dose of fludarabine (20 mg / m 2 / day~900mg / m 2 / day, about 10mg / m 2 / day~about 900mg / m 2 / day; for example, about 10 mg / m 2 / day, about 20mg / m 2 / day, about 30mg / m 2 / day, about 40mg / m 2 / day, about 40mg / m 2 / day, about 50mg / m 2 / day, about 60mg / m 2 / day, about 70mg / m 2 / day, about 80mg / m 2 / day, about 90mg / m 2 / day, about 100mg / m 2 / day, about 500mg / m 2 / day or approximately 900 mg / m 2 A preferred dosage regimen is about 300 mg / m2 / day prior to administering a therapeutically effective amount of engineered T cells to the patient. 2 / day cyclophosphamide and approximately 30 mg / m 2 Treatment of patients included administering fludarabine / day for 3 days.

[0245] In some embodiments, lymphodepletion further comprises administration of a CD52 antibody, hi some embodiments, the CD52 antibody is administered at an intravenous dose of about 13 mg / day.

[0246] In other embodiments, the antigen binding domain, the transduced (or otherwise engineered) cells, and the chemotherapeutic agent are each administered in an amount effective to treat the disease or condition of the subject.

[0247] In certain embodiments, the compositions comprising the CAR-expressing immune effector cells disclosed herein can be administered in conjunction with any number of chemotherapeutic agents, which can be administered in any order. Examples of chemotherapeutic agents include alkylating agents such as thiotepa and cyclophosphamide (CYTOXAN™); alkylsulfonates, such as busulfan, improsulfan, and piposulfan; aziridines, such as benzodopa, carboquone, meteuredopa, and uredopa; ethylenimines and methylameramines, including altretamine, triethylenemelamine, triethylenephosphamide, triethylenethiophosphamide, and trimethylolomelanin; nitrogen mustards, such as cyclophosphamide, ... nitrosoureas, such as chlorambucil, chlornaphazine, colofosfamide, estramustine, ifosfamide, mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, nobembicine, phenesterine, prednimustine, trofosfamide, uracil mustard, carmustine, chlorozotocin, fotemustine, lomustine, nimustine, ranimustine; antibiotics, such as arachnid, actinomycin, anthramycin, azaserine, bleomycin, sactinomycin, camomycin, Lithiamycin, carabicin, carminomycin, carzinophilin, chromomycin, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, doxorubicin, epirubicin, esorubicin, idarubicin, marcellomycin, mitomycin, mycophenolic acid, nogalamycin, olivomycin, peplomycin, potofilomycin, puromycin, queramycin, rodorubicin, streptonigrin, streptozocin, tubercidin, ubeni mex, zinostatin, zorubicin; antimetabolites such as methotrexate and 5-fluorouracil (5-FU); folic acid analogues such as denopterin, methotrexate, pteropterin, trimetrexate; purine analogues such as fludarabine, 6-mercaptopurine, thiamiprine, thioguanine; pyrimidine analogues such as ancitabine, azacitidine, 6-azauridine, carmoful, cytarabine, dideoxyuridine, doxifluridine, enocitabine, floxuridine, 5-FU;Androgens, e.g. calsterone, dromostanolone propionate, epithiostanol, mepitiostane, testosterone; antiadrenal, e.g. aminoglutethimide, mitotane, trilostane; folic acid supplements, e.g. furoic acid, aceglatone; aldophosphamide glycosides; aminolevulinic acid, amsacrine; bestravcil; bisantrene; edatraxate, defamin; demecolcine; diaziquone; elformitin; elliptinium acetate, etoglucide; gallium nitrate, hydroxyurea; lentinan; lonidamine; mitoguazone, mitoxantrone; mogamul; Pidamol; Nitracrine; Pentostatin; Phenamet; Pirarubicin; Podophyllic acid, 2-ethylhydrazide; Procarbazine; PSK®, Razoxane; Sizofiran; Spirogermanium; Tenuazonic acid, Triazicon; 2,2',2'-Trichlorotriethylamine; Urethane; Vindesine; Dacarbazine; Mannomustine; Mitobronitol; Mitalactol; Pipobroman; Gacytosine; Arabinoside (Ara-C), Cyclophosphamide; Thiotepa; Taxoids, e.g., Paclitaxel (TAXOL®, Bristol-Myers Squibb) and docetaxel (TAXOTERE®, Rhone-Poulenc Rorer); chlorambucil; gemcitabine; 6-thioguanine; platinum analogues such as mercaptopurine, methotrexate, cisplatin and carboplatin; vinblastine; platinum; etoposide (VP-16), ifosfamide; mitomycin C; mitoxantrone; vincristine; vinorelbine; navelbine; novantrone; teniposide; daunomycin; aminopterin; xeloda; ibandronate; CPT-11; topoisomerase inhibitors RF S2000; difluoromethylomitin (DMFO); retinoic acid derivatives such as Targretin™ (bexarotene), Panretin™, (alitretinoin); ONTAK™ (denileukin diftitox); esperamycin, capecitabine;and pharmacologic acceptable salts, acids, or derivatives of any of the foregoing. Included in this definition are, for example, antiestrogens, such as tamoxifen, raloxifene, aromatase-inhibiting 4(5)-imidazole, 4-hydroxytamoxifen, trioxyphene, keoxyphene, LY117018, onapristone, and toremifene (Fareston); and antihormonal agents that regulate or inhibit hormone action on tumors, such as antiandrogens, such as flutamide, nilutamide, bicalutamide, leuprolide, and goserelin; and pharmacologic acceptable salts, acids, or derivatives of any of the foregoing. Combinations of chemotherapeutic agents are also administered where appropriate, including, but not limited to, CHOP, i.e., cyclophosphamide (Cytoxan®), doxorubicin (hydroxydoxorubicin), vincristine (Oncovin®), and prednisone.;

[0248] In some embodiments, the chemotherapeutic agent is administered simultaneously or within one week after administration of the engineered cells, polypeptide, or nucleic acid. In other embodiments, the chemotherapeutic agent is administered 1-4 weeks, or 1 week-1 month, 1 week-2 months, 1 week-3 months, 1 week-6 months, 1 week-9 months, or 1 week-12 months after administration of the engineered cells, polypeptide, or nucleic acid. In other embodiments, the chemotherapeutic agent is administered at least one month prior to administration of the cells, polypeptide, or nucleic acid. In some embodiments, the method further comprises administering two or more chemotherapeutic agents.

[0249] A variety of additional therapeutic agents can be used in conjunction with the compositions described herein. For example, potentially useful additional therapeutic agents include PD-1 inhibitors such as nivolumab (Opdivo®), pembrolizumab (Keytruda®), pembrolizumab, pidilizumab, and atezolizumab.

[0250] Additional therapeutic agents suitable for use in combination with the present disclosure include, but are not limited to, ibrutinib (Imbruvica®), ofatumumab (Arzera®, rituximab (Rituxan®), bevacizumab (Avastin®), trastuzumab (Herceptin®), trastuzumab emtansine (Kadcyla®), imatinib ( Gleevec (registered trademark), cetuximab (Erbitux (registered trademark), panitumumab) (Vectibix (registered trademark), catumaxomab, ibritumomab, ofatumumab, tositumomab, brentuximab, alemtuzumab, gemtuzumab, erlotinib, gefitinib, vandetanib, afatinib, lapatinib, neratinib, axitinib, masitinib, pazopanib, sucralobacter Nitinib, sorafenib, toceranib, lestaurtinib, axitinib, cediranib, lenvatinib, nintedanib, pazopanib, regorafenib, semaxanib, sorafenib, sunitinib, tivozanib, toceranib, vandetanib, entrectinib, cabozantinib, imatinib, dasatinib, nilotinib, ponatinib, radotinib, bosutinib, lestaurtinib, ruxoli mTOR inhibitors such as tinib, pacritinib, cobimetinib, selumetinib, trametinib, binimetinib, alectinib, ceritinib, crizotinib, aflibercept, adipotide, denileukin diftitox, everolimus and temsirolimus, hedgehog inhibitors such as sonidegib and vismodegib, and CDK inhibitors such as the CDK inhibitor palbociclib.

[0251] In some embodiments, the composition comprising the CAR-containing immune cells can be administered with a therapeutic regimen to prevent cytokine release syndrome (CRS) or neurotoxicity. The therapeutic regimen to prevent cytokine release syndrome (CRS) or neurotoxicity can include lenzilumab, tocilizumab, atrial natriuretic peptide (ANP), anakinra, iNOS inhibitors (e.g., L-NIL or 1400W). In additional embodiments, the composition comprising the CAR-containing immune cells can be administered with an anti-inflammatory agent. Anti-inflammatory agents or drugs include, but are not limited to, steroids and glucocorticoids (betamethasone, budesonide, dexamethasone, hydrocortisone acetate, hydrocortisone, hydrocortisone, methylprednisolone, prednisolone, prednisone, triamcinolone), nonsteroidal anti-inflammatory drugs (NSAIDS) including aspirin, ibuprofen, naproxen, methotrexate, sulfasalazine, leflunomide, anti-TNF drugs, cyclophosphamide and mycophenolic acid. Exemplary NSAIDs include ibuprofen, naproxen, naproxen sodium, Cox-2 inhibitors, and sialic acid. Exemplary analgesics include acetaminophen, oxycodone, tramadol of proporoxifene hydrochloride. Exemplary glucocorticoids include cortisone, dexamethasone, hydrocortisone, methylprednisolone, prednisolone, or prednisone. Exemplary biological response modifiers include cell surface markers (e.g., CD4, CD5, etc.), cytokine inhibitors, such as TNF antagonists (e.g., etanercept (ENBREL®), Adminin (HUMIRA®), and infliximab (REMICADE®)), chemokine inhibitors, and adhesion molecule inhibitors. Biological response modifiers include monoclonal antibodies and recombinant forms of molecules. Exemplary DMARDs include azathioprine, cyclophosphamide, cyclosporine, methotrexate, penicillamine, leflunomide, sulfasalazine, hydroxychloroquine, gold (oral (auranofin) and intramuscular), and minocycline.

[0252] In certain embodiments, the compositions described herein are administered in conjunction with cytokines. Examples of cytokines include lymphokines, monokines, and conventional polypeptide hormones. Cytokines include growth hormones, such as human growth hormone, N-methionyl human growth hormone, and bovine growth hormone; parathyroid hormone; thyroxine; insulin; proinsulin; relaxin; prorelaxin; glycoprotein hormones, such as follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and luteinizing hormone (LH); hepatic growth factor (HGF); fibroblast growth factor (FGF); prolactin; placental lactogen; Mullerian inhibitor, mouse gonadotropin-related peptide; inhibin; activin; vascular endothelial growth factor; integrins; thrombopoietin (TPO); nerve growth factor (NGF), such as NGF-beta; platelet growth factor; TGF-alpha and TGF-beta. Transforming growth factors (TGF); insulin-like growth factors-I and -II; erythropoietin (EPO); bone morphogenetic factors; interferon-alpha, beta, and -gamma; colony stimulating factors (CSFs) such as macrophage-CSF (M-CSF); granulocyte-macrophage-CSF (GM-CSF); and granulocyte-CSF (G-CSF); interleukins (ILs), such as IL-1, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-15, IL-21; tumor necrosis factors such as TNF-alpha or TNF-beta; and other polypeptide factors, including LIF and Kit Ligand (KL). As used herein, the term cytokine includes proteins from natural sources or recombinant cell culture, as well as biologically active equivalents of the native sequence cytokines.

[0253] 7. Sorting and Depletion Methods In some embodiments, a method for in vitro sorting of an immune cell population is provided, where a subset of the immune cell population comprises engineered immune cells expressing an antigen-specific CAR that comprises an epitope (e.g., an exemplary mimotope sequence) specific for a monoclonal antibody. The method comprises contacting the immune cell population with a monoclonal antibody specific for the epitope and selecting immune cells that bind to the monoclonal antibody to obtain a cell population enriched for engineered immune cells expressing the antigen-specific CAR.

[0254] In some embodiments, the monoclonal antibody specific for the epitope is optionally conjugated to a fluorophore. In this embodiment, the step of selecting cells that bind to the monoclonal antibody can be performed by fluorescence-activated cell sorting (FACS).

[0255] In some embodiments, the monoclonal antibody specific for the epitope is optionally conjugated to a magnetic particle. In this embodiment, the step of selecting cells that bind to the monoclonal antibody can be performed by magnetic activated cell sorting (MACS).

[0256] In some embodiments, the mAb used in the methods of sorting immune cells expressing a CAR is selected from alemtuzumab, ibritumomab tiuxetan, muromonab-CD3, tositumomab, abciximab, basiliximab, brentuximab vedotin, cetuximab, infliximab, rituximab, bevacizumab, certolizumab pegol, daclizumab, eculizumab, efalizumab, gemtuzumab, natalizumab, omalizumab, palivizumab, ranibizumab, tocilizumab, trastuzumab, vedolizumab, admitamin, belimumab, canakinumab, denosumab, golimumab, ipilimumab, ofatumumab, panitumumab, QBEND-10, and / or ustekinumab. In some embodiments, the mAb is rituximab. In another embodiment, the mAb is QBEND-10. In another embodiment, the mAb binds to the TCR or TCR.

[0257] In some embodiments, the CAR-expressing immune cell population obtained when using the above-mentioned methods of in vitro sorting of CAR-expressing immune cells comprises at least 70%, 75%, 80%, 85%, 90%, 95% CAR-expressing immune cells. In some embodiments, the CAR-expressing immune cell population obtained when using the in vitro sorting methods of CAR-expressing immune cells comprises at least 85% CAR-expressing immune cells.

[0258] In some embodiments, the population of CAR-expressing immune cells obtained when using the above-mentioned in vitro sorting method of CAR-expressing immune cells exhibits increased cytotoxic activity in vitro compared to the initial (unsorted) cell population. In some embodiments, said cytotoxic activity is increased by 10%, 20%, 30%, or 50% in vitro. In some embodiments, the immune cells are T cells.

[0259] In some embodiments, the mAb is previously bound onto a support or surface. Non-limiting examples of solid supports can include beads, agarose beads, magnetic beads, plastic bonded plates, glass bonded plates, ceramic bonded plates, columns, or cell culture bags.

[0260] The CAR-expressing immune cells administered to the recipient can be enriched in vitro from a source population. Methods for expanding the source population can include using a combination of density centrifugation, immunomagnetic bead purification, affinity chromatography, and fluorescence-activated cell sorting to select cells that express an antigen, such as the CD34 antigen.

[0261] Flow cytometry can be used to quantify specific cell types in a cell population.Generally, flow cytometry is a method for quantifying the components or structural features of cells mainly by optical means.By quantifying structural features, different cell types can be distinguished, so that flow cytometry and cell sorting can be used to count and sort cells of different phenotypes in a mixture.

[0262] Flow cytometry analysis involves two major steps: 1) labeling a selected cell type with one or more labeling markers, and T) determining the number of labeled cells relative to the total number of cells in a population. In some embodiments, the method of labeling a cell type involves binding a labeled antibody to a marker expressed by a particular cell type. The antibody can be directly labeled with a fluorescent compound or indirectly labeled, for example, with a fluorescently labeled second antibody that recognizes the first antibody.

[0263] In some embodiments, the method used to sort T cells expressing CAR is magnetic activated cell sorting (MACS). Magnetic activated cell sorting (MACS) is a method for separating different cell populations according to their surface antigens (CD molecules) using superparamagnetic nanoparticles and columns. Using MACS, pure cell populations can be obtained. Cells in single cell suspension can be magnetically labeled with microbeads. The sample is applied to a column made of ferromagnetic spheres, covered with a cell-friendly coating, allowing for a fast and gentle separation of cells. Unlabeled cells pass through, while magnetically labeled cells are retained in the column. The flow-through can be collected as the unlabeled cell fraction. After a washing step, the column is removed from the separator and the magnetically labeled cells are eluted from the column.

[0264] Detailed protocols for purification of specific cell populations such as T cells can be found in Basu S et al. (2010). See (Basu S, Campbell HM, Dittel BN, Ray A. Purification of specific cell population by fluorescence activated cell sorting (FACS). J Vis Exp. (41): 1546).

[0265] In some aspects, the present disclosure provides a method for depleting antigen-specific CAR-expressing immune cells by in vivo depletion. In vivo depletion can include administering a therapeutic agent (e.g., a molecule that binds to an epitope on a CAR) to a mammalian organism, aiming to stop the proliferation of CAR-expressing immune cells by inhibition or elimination.

[0266] One aspect of the disclosure relates to a method for in vivo depletion of engineered immune cells expressing a CAR comprising a mAb-specific epitope, comprising contacting said engineered immune cells or said CAR-expressing immune cells with at least one epitope-specific mAb. Another aspect of the disclosure relates to a method for in vivo depletion of CAR-expressing immune cells comprising a chimeric scFv (e.g., formed by insertion of a mAb-specific epitope) by contacting said engineered immune cells with an epitope-specific antibody. In some embodiments, the immune cells are T cells and / or the antibody is a monoclonal antibody.

[0267] According to one embodiment, in vivo depletion of immune engineered cells is performed on engineered immune cells previously sorted using the in vitro method of the present disclosure. In this case, an infused mAb can be used. In some embodiments, the mAb specific antigen is CD20 antigen and the epitope specific mAb is rituximab. In some embodiments, the disclosure relates to a method of in vivo depletion of engineered immune cells expressing a CAR containing a mAb specific epitope (CAR expressing immune cells) in a patient, comprising contacting said CAR expressing immune cells with at least one epitope specific mAb.

[0268] In some embodiments, contacting the engineered immune cells or the CAR-expressing immune cells with at least one epitope-specific mAb comprises injecting the patient with the epitope-specific mAb (e.g., rituximab). In some embodiments, the amount of epitope-specific mAb administered to the patient is sufficient to eliminate at least 20%, 30%, 40%, 50%, 60%, 70%, 80%, or 90% of the CAR-expressing immune cells in the patient.

[0269] In some embodiments, contacting the engineered immune cells or the CAR-expressing immune cells with at least one epitope-specific mAb comprises administering to the patient 375 mg / m 2 In some embodiments, the mAb (e.g., rituximab) is administered once a week.

[0270] In some embodiments, when immune cells expressing a CAR containing a mAb-specific epitope (CAR-expressing immune cells) are depleted in a complement-dependent cytotoxicity (CDC) assay using an epitope-specific mAb, the amount of viable CAR-expressing immune cells is reduced. In some embodiments, the amount of viable CAR-expressing immune cells is reduced by at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, or 90%. In some embodiments, the mAb-specific epitope is a CD20 epitope or mimotope, and / or the epitope-specific mAb is rituximab.

[0271] In certain embodiments, in vivo depletion of CAR-engineered immune cells is performed by injecting bispecific antibodies. By definition, bispecific monoclonal antibodies (BsAbs) are engineered proteins that are composed of fragments of two different monoclonal antibodies, and as a result bind to two different types of antigens. These BsAbs and their use in immunotherapy are outlined in Muller D and Kontermann RE (2010) Bispecific Antibodies for Cancer Immunotherapy, BioDrugs 24(2):89-98.

[0272] According to another particular embodiment, the injected bispecific mAb can bind both the mAb-specific epitope carried by the engineered immune cells expressing the chimeric scFv and the surface antigen of effector and cytotoxic cells (e.g., immune cells such as lymphocytes, macrophages, dendritic cells, natural killer cells (NK cells), cytotoxic T lymphocytes (CTLs)). In doing so, the depletion of engineered immune cells induced by BsAb can occur via antibody-dependent cellular cytotoxicity (ADCC). (Deo YM, Sundarapandiyan K, Keler T, Wallace PK, and Graziano RF, (2000), Journal of Immunology, 165(10):5954-5961]).

[0273] In some embodiments, cytotoxic agents are linked to epitope-specific mAbs that can be used to deplete CAR-expressing immune cells.By combining the targeting ability of monoclonal antibodies with the cancer-killing ability of cytotoxic drugs, antibody-drug conjugates (ADCs) allow for the differentiation of sensitivity between healthy and diseased tissues compared to the use of drugs alone.Several ADCs have been approved for the market, and the techniques for making them, especially on linkers, have been described (Payne, G. (2003) Cancer Cell 3:207-212; Trail et al. (2003) Cancer Immunol. Immunother. 52:328-337; Syrigos and Epeneto (1999) Anticancer Research 19:605-614; Niculescu-Duvaz and Springer (1997) Adv. Drug Del. Rev. 26:151-172, U.S. Patent No. 4,975,278).

[0274] In some embodiments, the epitope-specific mAb injected is pre-conjugated with a molecule that can promote complement-dependent cytotoxicity (CDC). Thus, the complement system aids or complements the ability of antibodies to clear pathogens from the organism. When stimulated, an activation cascade is driven as a massive amplification of the response and activation of cell-killing membrane attack complexes. Different molecules can be used to bind mAbs, such as glycans [Courtois, A, Gac-Breton, S., Berthou, C, Guezennec, J., Bordron, A. and Boisset, C. (2012), Complement dependent cytotoxicity activity of therapeutic antibody fragments can be acquired by immunogenic glycan coupling, Electronic Journal of Biotechnology ISSN: 0717-3458; http: / / www.ejbiotechnology.info DOI: 10.2225 / voll5-issue5).

[0275] 8. Kits and Articles of Manufacture The present disclosure provides kits comprising any of the cultured or engineered immune cells described herein, as well as pharmaceutical compositions thereof. In some exemplary embodiments, the kits of the present disclosure comprise allogeneic CAR-containing T cells for administration to a subject.

[0276] The present application also provides an article of manufacture comprising any one of the therapeutic compositions or kits described herein. An example of an article of manufacture is a vial (e.g., a sealed vial).

[0277] Additionally, the disclosure provides kits that include one or more containers containing a solution of potassium at a desired concentration, and, optionally, one or more containers containing a solution containing one or more cytokines, such as IL-7 or IL-15. EXAMPLES

[0278] As shown in the examples below, the combination of cytokine and metabolic regulator conditions and concentrations in the T cell growth medium can result in increased efficacy of genetically modified allogeneic cell therapy products. For example, the desired allogeneic CAR T cell phenotype can be obtained by the use of stimulants of cell proliferation, such as IL-7, IL-15, and increased extracellular potassium.

[0279] 1. Exemplary Protocol for CAR T Cell Production As described herein, CAR T cells can be produced according to a variety of methods known in the art. Exemplary standard methods are described herein.

[0280] To generate CAR-T cells, PBMCs can be purified from buffy coat samples of healthy volunteers using Ficoll gradient density medium (Ficoll Paque PLUS / GE Healthcare Life Sciences). T cells can be purified from PBMCs using a commercially available T cell isolation kit (Miltenyi Biotec, Cat. No. 130-096-535). Alternatively, primary human T cells can be directly purified from Leuko Paks (StemCell Technologies).

[0281] To generate lentivirus encoding CAR, HEK-293T cells can be seeded at 400,000 cells / mL on day 0 in 2 mL of DMEM (Gibco) supplemented with 10% FBS (Hyclone or JR Scientific) per well of a 6-well plate. On day 1, lentivirus can be prepared by mixing lentiviral packaging vectors 1.5ug psPAX2, 0.5ug pMD2G, and 0.5ug of the appropriate transfected CAR vector in 250uL of Opti-MEM (Gibco) per well of a 6-well plate ("DNA mix"). 10uL of Lipofectamine 2000 (Invitrogen) in 250uL of Opti-MEM can be incubated at room temperature for 5 minutes and then added to the DNA mix. The mixture can be incubated at room temperature for 20 minutes and the total volume of 500uL slowly added to the side of the well containing the HEK-293T.

[0282] Purified T cells can be activated in appropriate medium. On day 2, medium from each well of a 6-well plate can be replaced with 2 mL per well of T cell transduction medium, i.e., X-Vivo-15 supplemented with 10% FBS. On day 3, T cells can be resuspended at 500,000 cells per mL in 1 mL of T cell transduction medium per well of a Grex-24 plate (Wilson Wolf, Cat. No. 80192M). Lentiviral supernatant from HEK293T cells can be harvested and passed through a 0.45 micron filter (EMD Millipore) to remove cell debris, then added to the T cells along with 100 IU / mL of human IL-2.

[0283] On day 5, 4.5 mL of T cell expansion medium (e.g., containing IL-7+IL-15, with or without additional extracellular potassium) can be added to each well of the Grex-24 plate. On days 9 and 13, transduction efficiency can be determined by detecting the percentage of T cells that recognize the desired antigen (e.g., recombinant antigen) using flow cytometry. Cells were expanded into larger flasks or G-Rex vessels (Wilson Wolf) as needed using T cell expansion medium as described herein.

[0284] On day 14, antigen-specific CAR-T cells can be cryopreserved by freezing the cells in medium such as, for example, CryoStor® CS5, CryoStor® CS10, or CryoStor® CS2 (BioLife Solutions). The percentage of cells stained with recombinant antigen can be normalized between clones immediately prior to cryopreservation.

[0285] 2.IL-7+15 supplementation The data in the examples below show, for example, that the use of first and second stimulatory agents, e.g., IL-7 (5,000 IU / ml) and IL-15 (50 IU / ml), optionally with increased extracellular potassium (25 mM), can achieve a highly desirable phenotype of allogeneic CAR T cells compared to classical IL-2-based processes.

[0286] We first tested supplementation of IL-7 and IL-15 in cell culture media with potassium maintained at a baseline level of 4 mM. As shown in Figure 1A, when benchmarking to an IL-2-based manufacturing process ("IL-2 → IL-2" - fresh IL-2 added to 100 IU / mL of IL-2 on day 5), an IL-7+IL-15-based process ("IL-2 → IL-7+15" - 100 IU / mL of IL-2 added by day 2, 5000 IU / mL of IL-7 and 50 IU / mL of IL-15 on day 5, or "IL-7+15 → IL-7+15" - fresh IL-7 and IL-15 added to 5000 IU / mL of IL-7 and 50 IU / mL of IL-15 on day 5) significantly improved T cell proliferation of CD19-specific CAR T cells in the final product. SCM( CD62L + CD45RO - As shown in Figure 1B, IL-7+15 culture conditions also increased the cytokine release capacity of CD19-specific CAR T cells upon target cell challenge.

[0287] 3. Potassium supplementation and titration test To further improve the potency of allogeneic CAR T cells, we investigated combining the benefits of IL-7+15 supplementation at the same concentrations as in previous experiments with extracellular nutrients known to regulate T cell metabolism. Extracellular potassium has been shown to be an inhibitory mechanism by which the tumor microenvironment (TME) suppresses effector T cells in vivo. On the other hand, increased extracellular potassium during in vitro expansion for adoptive cell therapy (ACT) leads to the preservation of T cells with a younger, less differentiated phenotype.

[0288] As shown in Figure 2A, increased extracellular potassium (40 mM, open bars) significantly increased T cell proliferation and T cell proliferation in allogeneic CD19 CAR T cells during both IL-2 and IL-7+15 steps compared to normal (4 mM) potassium concentrations (filled bars). SCM However, in Figure 2B, we unexpectedly found that conditions of increased extracellular potassium (40 mM, open bars) compared to normal potassium concentrations (4 mM, filled bars) negatively impacted the proliferation capacity of allogeneic CAR T cells in both IL-2 and IL-7+15-based processes.

[0289] To further strengthen the benefits of high extracellular potassium during allogeneic CAR T cell manufacturing, we adjusted the amount of extracellular potassium, which is especially effective for IL-7+15-based allogeneic CAR T cell processes. Extracellular potassium concentrations of 25 mM and 10 mM did not negatively affect the proliferation of human T cells during allogeneic CAR T cell manufacturing (Figure 3). Furthermore, at 25 mM extracellular potassium (Figure 4), T SCM Maximal cell conservation was achieved.

[0290] 4. Performance testing using combination supplementation with IL-7+15 and potassium The sequential killing assay allows CAR-T cells to expand and, in certain cases, differentiate and become exhausted by repeatedly exposing them to targets. This assay was used to test the ability of allogeneic CAR T cells expanded under different conditions.

[0291] Having identified an effective concentration of extracellular potassium for allogeneic CAR T cell manufacturing, we proceeded to evaluate whether the combination of IL-7+15 with a potassium concentration of 25 mM would improve the potency of allogeneic CAR T cells. The combination of an IL-7+15-based process with 25 mM extracellular potassium supplementation results in the greatest potency of CAR T cells compared to a similar product manufactured with normal (4 mM) potassium concentration in IL-2 (Figure 5). Furthermore, the combination of an IL-7+15-based process with 25 mM extracellular potassium significantly improved potency compared to an IL-2-based process with 25 mM potassium, which further improved potency compared to an IL-7+15-based process with a baseline level of potassium of 4 mM.

[0292] In conclusion, we identified a highly effective combination of cytokines (IL-7+IL-15) and metabolic regulators (extracellular potassium) that can maximize the potency of allogeneic CAR T cells.

[0293] Although the teachings of the present disclosure have been described with reference to various applications, methods, kits, and compositions, it will be understood that various changes and modifications can be made without departing from the teachings of the present specification and the following claimed invention. The foregoing examples are provided to better illustrate the disclosed teachings and are not intended to limit the scope of the teachings presented herein. Although the present teachings have been described in terms of these exemplary embodiments, those skilled in the art will readily appreciate that numerous variations and modifications of these exemplary embodiments are possible without undue experimentation. All such variations and modifications are within the scope of the teachings of the present invention. [Table 3] TIFF0007650818000004.tif224170

Claims

1. 1. A cell growth medium for T cell expansion, comprising: IL-7 at a concentration of 3000 IU / mL to 5000 IU / mL and IL-15 at a concentration of 1 IU / mL to 100 IU / mL, and an extracellular regulator of cellular metabolism, which is extracellular potassium at a concentration between 10 mM and 35 mM; Including, A cell growth medium in which IL-7 and IL-15 are present at a concentration ratio of 1000:1 to 4:1, with concentrations in IU / mL.

2. 2. The cell growth medium of claim 1, wherein IL-7 and IL-15 are present in a concentration ratio of 500:1 to 4:1 and at a concentration of IU / mL.

3. 2. The cell growth medium of claim 1, wherein IL-7 and IL-15 are present in a concentration ratio of 150:1 to 50:1, and at a concentration of IU / mL.

4. 2. The cell growth medium of claim 1, wherein IL-15 is present at a concentration of 25 IU / mL to 50 IU / mL.

5. 5. The cell growth medium of claim 4, wherein IL-7 is present at a concentration of 5000 IU / mL and IL-15 is present at a concentration of 50 IU / mL.

6. 6. The cell growth medium of claim 1, wherein the extracellular potassium is present at a concentration of 20 mM to 35 mM.

7. 6. The cell growth medium of claim 1, wherein the extracellular potassium is present at a concentration of 10 mM to 25 mM.

8. 8. The cell growth medium of claim 7, wherein the extracellular potassium is present at a concentration of 15 mM, 20 mM or 25 mM.

9. The cell growth medium of any one of claims 1 to 8, wherein the extracellular potassium is present as KCl.

10. 1. A method for obtaining a population of T cells in vitro, comprising: IL-7 at a concentration of 3000 IU / mL to 5000 IU / mL and IL-15 at a concentration of 1 IU / mL to 100 IU / mL, and culturing the initial population of T cells in a cell growth medium containing an extracellular regulator of cellular metabolism, the extracellular potassium being at a concentration of 10 mM to 35 mM; The method, wherein IL-7 and said IL-15 are present in a concentration ratio of 1000:1 to 4:1, and the concentrations are in IU / mL.

11. The method according to claim 10, wherein the cell growth medium is a cell growth medium according to any one of claims 1 to 9.

12. The obtained T cell population is CM Cells and / or T SCM The method of claim 10, wherein the cells are enriched.

13. The obtained T cell population is at least 30% T SCM The method of claim 12 comprising a cell.

14. The obtained T cell population is at least 40% T SCM The method of claim 13 comprising a cell.

15. The obtained T cell population exhibits a T cell proliferation rate of 1.0-1.2 times that of the initial population of T cells when measured over a period of 7-16 days. CM and / or T SCM From T CM and / or T SCM The method of claim 10, wherein is enriched 100-fold to 1000-fold.

16. The obtained T cell population exhibits a T cell proliferation rate of 10-14 days, which is higher than that of the initial population of T cells. CM and / or T SCM From T CM and / or T SCM The method of claim 15, wherein is enriched 100 to 1000 fold.

17. The obtained population of T cells may be a T cell population of the initial population of T cells. CM and / or T SCM From T CM and / or T SCM The method of claim 16, wherein is enriched at least 100-fold or 200-fold.

18. 11. The method of claim 10, wherein the initial population of T cells is an engineered T cell population.

19. 20. The method of claim 18, wherein the initial population of T cells is a T cell population that expresses one or more chimeric antigen receptors.

20. 20. The method of claim 19, wherein the T cells are allogeneic T cells.

21. 20. The method of claim 19, wherein the T cells are autologous T cells.

22. 11. The method of claim 10, wherein IL-7 and IL-15 are present in a concentration ratio of 500:1 to 4:1, and the concentrations are in IU / mL.

23. 11. The method of claim 10, wherein IL-7 and IL-15 are present in a concentration ratio of 150:1 to 50:1, and the concentrations are in IU / mL.

24. The method of claim 10, wherein IL-15 is present at a concentration of 25 IU / mL to 50 IU / mL.

25. 11. The method of claim 10, wherein IL-7 is present at a concentration of 5000 IU / mL and IL-15 is present at a concentration of 50 IU / mL.

26. 26. The method of any one of claims 10 to 25, wherein the extracellular potassium is present at a concentration of 20 mM to 35 mM.

27. 26. The method of any one of claims 10 to 25, wherein the extracellular potassium is present at a concentration of 10 mM to 25 mM.

28. 28. The method of claim 27, wherein the extracellular potassium is present at a concentration of 15 mM, 20 mM or 25 mM.

29. 29. The method of any one of claims 10 to 28, wherein the extracellular potassium is present as KCl.

30. The method of any one of claims 10 to 29, wherein the T cells are isolated from peripheral blood mononuclear cells (PBMCs).

31. 1. A cell growth medium for T cell expansion, comprising: IL-7 at a concentration of 3000 IU / mL to 5000 IU / mL and IL-15 at a concentration of 1 IU / mL to 100 IU / mL, and an extracellular regulator of cellular metabolism, which is extracellular potassium at a concentration between 10 mM and 35 mM; Including, A cell growth medium in which IL-7 and IL-15 are present at a concentration ratio of 500:1 to 10:1, with a concentration of IU / mL.

32. 32. The cell growth medium of claim 31, wherein IL-15 is present at a concentration of 25 IU / mL to 50 IU / mL.

33. 33. The cell growth medium of claim 31 or 32, wherein the extracellular potassium is present at a concentration of 15 mM, 20 mM or 25 mM.

34. The cell growth medium of any one of claims 31 to 33, wherein the extracellular potassium is present as KCl.

35. 1. A method of obtaining a population of T cells in vitro, comprising culturing an initial population of T cells in a cell expansion medium, the cell expansion medium comprising: IL-7 at a concentration of 3000 IU / mL to 5000 IU / mL and IL-15 at a concentration of 1 IU / mL to 100 IU / mL, and an extracellular regulator of cellular metabolism, which is extracellular potassium at a concentration between 10 mM and 35 mM; Including, The method wherein IL-7 and IL-15 are present in a concentration ratio of 500:1 to 50:1, wherein the concentrations are in IU / mL.

36. 36. The method of claim 35, wherein IL-7 and IL-15 are present in a concentration ratio of 150:1 to 50:1, and the concentrations are in IU / mL.

37. 36. The method of claim 35, wherein IL-15 is present at a concentration of 25 IU / mL to 50 IU / mL.

38. 36. The method of claim 35, wherein IL-7 is present at a concentration of 5000 IU / mL and IL-15 is present at a concentration of 50 IU / mL.

39. 39. The method of any one of claims 35 to 38, wherein the extracellular potassium is present at a concentration of 20 mM to 35 mM.

40. 39. The method of any one of claims 35 to 38, wherein the extracellular potassium is present at a concentration of 10 mM to 25 mM.

41. 41. The method of claim 40, wherein the extracellular potassium is present at a concentration of 15 mM, 20 mM or 25 mM.

42. 42. The method of any one of claims 35 to 41, wherein the extracellular potassium is present as KCl.

43. The method of any one of claims 35 to 42, wherein the T cells are isolated from peripheral blood mononuclear cells (PBMCs).

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