TRK kinase inhibitor compounds and their uses

Abstract

This application relates to TRK kinase inhibitor compounds and their uses.Specifically, this application discloses a compound of formula (I), or an isotopically labeled compound thereof, or an optical isomer, a geometric isomer, a tautomer or a mixture of isomers thereof, or a pharma- ceutically acceptable salt thereof, a prodrug thereof, or a metabolite thereof.This application further relates to the medical use of said compound. [Formula 1] TIFF2024524236000059.tif45152

Description

[Technical Field]

[0001] The present application provides novel compounds having pharmacological activity, which can inhibit the activity of TRK kinase. The present application further relates to compositions containing the compounds and the use of the compounds and compositions in the manufacture of medicaments for treating diseases or conditions associated with TRK kinase or NTRK genes. [Background technology]

[0002] Protein kinases (PKs) are important regulators of cell growth, proliferation, and survival, and their dysfunction is a hallmark of many diseases. The majority of oncogenes and proto-oncogenes associated with human cancer encode PKs. In recent years, one type of protein kinase, TRK kinase (tropomyosin receptor kinase), has attracted the attention of researchers.

[0003] TRK kinases belong to the receptor tyrosine kinase family. The Trk family mainly includes three members, TRKA, TRKB, and TRKC, which are encoded by the neurotrophic receptor tyrosine kinase (NTRK) genes: NTRK1, NTRK2, and NTRK3, respectively. Previous studies have demonstrated that these TRK kinases, as receptors for nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophic factor 4 / 5 (NT-4 / 5), and neurotrophic factor 3 (NT-3), can regulate neuronal cell signaling, cell proliferation, differentiation, metabolism, and even apoptosis. TRK kinases are constitutively activated in human malignant tumors through multiple mechanisms. The most well-established mechanism is NTRK gene fusion, in which the 3′ NTRK gene region is rearranged intrachromosomally or interchromosomally, fusing the 5′ region with a partner gene sequence, increasing the risk of tumor development. Activation or disruption of TRK kinases and NTRK gene fusions have been shown to be closely associated with the development, progression, and progression of various tumors and cancers. For patients with NTRK fusion gene expression, TRK is an important target for tumor therapy. Therefore, small molecule TRK kinase inhibitors are considered to be potential broad-spectrum anticancer drugs. TRK inhibitors have also been shown to be effective in preclinical animal models of pain and inflammation.

[0004] Larotrectinib, also known as LOXO-101 or Vitrakvi, is the first TRK inhibitor anticancer drug to be approved for public listing. It has demonstrated significant therapeutic efficacy in the treatment of several cancers, but its price is prohibitive and it is difficult to overcome drug resistance after long-term use. The second TRK inhibitor to be approved for public listing is entrectinib, also known as Rozlytrek, which also demonstrates good broad-spectrum anticancer activity.

[0005] Researchers have recognized the importance and potential of TRK inhibitors in cancer treatment based on the success of larotrectinib and entrectinib, but currently there is still a strong desire for other alternative TRK inhibitors. Summary of the Invention

[0006] In a first aspect, the present application provides a compound of formula (I), or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or isomeric mixture thereof, or a pharmaceutically acceptable salt thereof, a prodrug thereof, or a metabolite thereof, as a TRK kinase inhibitor.

[0007] [ka] (In the formula, R 1 , R 2 are H, CN, and C 1-4 Alkyl, C 1-4 Alkyl halides, C 1-4 are independently selected from alkoxy and halogen; R 3 is H, C 1-4 Alkyl, C 1-4 Alkyl halides, C 1-4 Alkoxy, C 3-6 selected from an alicyclic group and a 4- to 6-membered heteroalicyclic group; R 6 The number of R is 1, 2 or 3, and each R 6is H, halogen, -CN, -OH, -NO2, -NR 7 R 8 , C 1-4 Alkyl, C 1-4 Alkyl halides, C 1-4 Alkoxy, C 3-6 are independently selected from an alicyclic group and a 4- to 6-membered heteroalicyclic group; X is a connecting bond, O, S or (NR 4 ), where R 4 is H, C 1-4 Alkyl, C 1-4 Alkyl halides, C 1-4 Alkoxy, C 3-6 selected from an alicyclic group and a 4- to 6-membered heteroalicyclic group; R 7 and R 8 is H, C 1-6 Alkyl, C 1-4 Alkyl halides, C 1-4 alkoxy, or R 7 and R 8 form a 3- to 6-membered ring together with the N atom to which they are linked, n=1, 2 or 3; L is (C=O), (O=S=O), CR a R b or a connecting bond, where R a and R b is H, C 1-4 Alkyl, C 1-4 Alkyl halides, C 1-4 Alkoxy, C 3-6 are independently selected from an alicyclic group and a 4- to 6-membered heteroalicyclic group, or R a and R b form a 3- to 6-membered ring together with the carbon atoms to which they are attached, R 5 is H, halogen, -OH, -NO2, -CN, -SF5, -SH, -SC 1-4 Alkyl, C 1-6 Alkyl, C 1-6 Alkyl halides, C 1-6 Alkoxy, C 1-6 Halogenated alkoxy, C 2-8Alkenyl, C 2-8 Alkynyl, C 3-7 Alicyclic group, 3- to 10-membered heteroalicyclic group, C 6-12 Dicycloaliphatic groups, 6- to 12-membered dicycloheteroalicyclic groups, C 8―15 8-15-membered tricycloaliphatic group, 8-15-membered tricycloheteroaliphatic group, C 5-8 Aryl, 5-10 membered heteroaryl, C 7-11 Dicycloaryl, 7-11 membered dicycloheteroaryl, -C 1-4 Alkyl-(C 3-7 alicyclic group), -C 1-4 Alkyl-(3- to 10-membered heteroalicyclic group), -C 1-4 Alkyl-(C 6-12 Dicycloaliphatic group), -C 1-4 Alkyl-(6- to 12-membered dicycloheteroalicyclic group), -C 1-4 Alkyl-(C 8―15 -membered tricycloaliphatic group), -C 1-4 Alkyl-(8- to 15-membered tricycloheteroalicyclic group), -C 1-4 Alkyl-(C 5-8 aryl), -C 1-4 Alkyl-(5-10 membered heteroaryl), -N(R 10 )(R 11 ), -N(R 10 )(C(=O)R 11 ), -N(R 10 )(C(=O)-OR 11 ), -N(R 12 )(C(=O)-N(R 10 )(R 11 )), -C(=O)-N(R 10 )(R 11 ), -C(=O)-R 12 , -C(=O)-OR 12 , -OC(=O)R 12 , -N(R 10 )(S(=O)2R 11 ), -S(=O)2-N(R 10 )(R 11 ), -SR 12 and -OR 12 wherein the above-SC 1-4 Alkyl, C 1-6 Alkyl, C1-6 Alkoxy, C 1-6 Halogenated alkoxy, C 2-8 Alkenyl, C 2-8 Alkynyl, C 3-7 Alicyclic group, 3- to 10-membered heteroalicyclic group, C 6-12 Dicycloaliphatic groups, 6- to 12-membered dicycloheteroalicyclic groups, C 8―15 8-15-membered tricycloaliphatic group, 8-15-membered tricycloheteroaliphatic group, C 5-8 Aryl, 5-7 membered heteroaryl, C 7-11 Dicycloaryl, 7-11 membered dicycloheteroaryl, -C 1-4 Alkyl-(C 3-7 alicyclic group), -C 1-4 Alkyl-(3- to 10-membered heteroalicyclic group), -C 1-4 Alkyl-(C 6-12 Dicycloaliphatic group), -C 1-4 Alkyl-(6- to 12-membered dicycloheteroalicyclic group), -C 1-4 Alkyl-(C 8―15 -membered tricycloaliphatic group), -C 1-4 Alkyl-(8- to 15-membered tricycloheteroalicyclic group), -C 1-4 Alkyl-(C 5-8 aryl) and -C 1-4 Alkyl-(5-10 membered heteroaryl) is 0, 1, 2, 3 or 4 R 5a are optionally substituted with R 5a are halogens, -OH, -NO2, -CN, -SF5, -SH, -SC 1-4 Alkyl, oxo, C 1-4 Alkyl, C 1-4 Alkyl halides, C 1-4 Alkoxy, C 1-4 Halogenated alkoxy, C 2-8 Alkenyl, C 2-8 Alkynyl, C 3-7 Alicyclic group, 3- to 10-membered heteroalicyclic group, C 5-8 Aryl, 5- to 7-membered heteroaryl, -N(R 13 )(R 14 ), -N(R 13 )(C(=O)R 14 ), -N(R 13 )(C(=O)-OR14 ), -N(R 15 )(C(=O)-N(R 13 )(R 14 )), -C(=O)-N(R 13 )(R 14 ), -C(=O)-R 15 , -C(=O)-OR 15 , -OC(=O)R 15 , -N(R 13 )(S(=O)2R 14 ), -S(=O)2-N(R 13 )(R 14 ), -SR 15 and -OR 15 are independently selected from R 10 , R 11 , R 12 , R 13 , R 14 and R 15 For each occurrence, H, C 1-6 Alkyl, C 1-6 Alkyl halides, C 3-7 Alicyclic group, 3- to 10-membered heteroalicyclic group, C 5-8 Aryl, 5-7 membered heteroaryl, C 7-11 Dicycloaryl, 7-11 membered dicycloheteroaryl, -C 1-4 Alkyl-(C 3-7 alicyclic group), -C 1-4 Alkyl-(3- to 10-membered heteroalicyclic group), -C 1-4 Alkyl-(C 6-12 Dicycloaliphatic group), -C 1-4 Alkyl-(6- to 12-membered dicycloaliphatic group), -C 1-4 Alkyl-(C 8-15 -membered tricycloaliphatic group), -C 1-4 Alkyl-(8- to 15-membered tricycloaliphatic group), -C 1-4 Alkyl-(C 5-8 aryl) and -C 1-4 alkyl-(5-10 membered heteroaryl), where each option within the group is selected from halogen, —OH, —NH2, —NH(CH3), —N(CH3)2, —CN, —NO2, —SF5, —SH, —SC 1-4 Alkyl, oxo, C 1-4Alkyl, C 2-6 Alkenyl, C 2-6 Alkynyl, C 3-7 Alicyclic group, 3- to 10-membered heteroalicyclic group, C 5-8 Aryl, 5-7 membered heteroaryl, C 7-11 Dicycloaryl, 7-11 membered dicycloheteroaryl, C 1-4 Hydroxyalkyl, -SC 1-4 Alkyl, -C(=O)H, -C(=O)-C 1-4 Alkyl, -C(=O)-OC 1-4 Alkyl, -C(=O)-NH2, -C(=O)-N(C 1-4 Alkyl)2, C 1-4 Alkyl halides, C 1-4 Alkoxy and C 1-4 optionally substituted with 0, 1, 2, 3, or 4 substituents each independently selected from the group consisting of halogenated alkoxy, or R 10 and R 11 form a 3- to 14-membered ring together with the atoms to which they are connected, or R 13 and R 14 form a 3- to 14-membered ring together with the atoms to which they are connected. DETAILED DESCRIPTION OF THE INVENTION

[0008] Unless otherwise specified, any reference herein to a "compound of formula (I)," "compound of formula (I)," "compound of the present application," or similar term also includes any optical isomers, geometric isomers, tautomers, or mixtures of isomers thereof.

[0009] The term "optical isomer" means that when a compound has one or more chiral centers, each chiral center can have an R or S configuration, and the various isomers formed thereby are optical isomers. Optical isomers include all diastereomers, enantiomers, meso forms, racemates, or mixtures thereof. For example, optical isomers can be separated by chiral chromatography columns or chiral synthesis.

[0010] The term "geometric isomer" means that when a compound has a double bond, the compound exists as cis, trans, E, and Z isomers. Geometric isomers include cis, trans, E, Z isomers or mixtures thereof.

[0011] The term "tautomer" refers to an isomer formed by the rapid displacement of an atom at two positions within a molecule. Those skilled in the art will understand that tautomers can be converted into each other and that under certain conditions, they can reach a state of equilibrium and coexist.

[0012] Unless otherwise specified, the terms "compounds represented by formula (I)," "compounds of formula (I)," "compounds of the present application," and the like used herein also include isotopically labeled compounds in which one or more atoms in the compound are replaced with their isotope atoms.

[0013] Examples of isotopes that may be present in compounds of this application include isotopes of hydrogen (e.g., 2 H(D) and 3 H(T)), isotopes of carbon (e.g., 11 C. 13 C and 14 C), isotopes of chlorine (e.g., 36 Cl), isotopes of fluorine (e.g., 18 F), isotopes of iodine (e.g., 123 I and 125 I), isotopes of nitrogen (e.g., 13 N and 15 N), isotopes of oxygen (e.g., 15 O. 17 O and 18 O), and isotopes of sulfur (e.g., 35 S).

[0014] Isotopically labeled compounds (e.g., compounds containing radioactive isotopes) can be used in drug and / or substrate tissue distribution studies. For ease of incorporation and convenient means of detection, the radioactive isotopes deuterium (i.e., D) and carbon-14 (i.e., 14 C) is particularly useful for this purpose.

[0015] Substitution with heavier isotopes such as deuterium (i.e., D) can provide several therapeutic advantages and is therefore preferred in certain circumstances. Therapeutic advantages may result, for example, from greater metabolic stability (e.g., increased in vivo half-life or reduced dose requirements). Thus, in some embodiments, the compounds of the present application are isotopically labeled compounds, where H, at each occurrence, is optionally replaced with D.

[0016] Positron-emitting isotopes (e.g., 11 C. 18 F, 15 O and 13 N) can be used in Positron Emission Topography (PET) studies to detect substrate receptor occupancy.

[0017] Isotopically labeled compounds can generally be prepared by conventional techniques known to those skilled in the art, or by substituting an appropriate isotopically labeled reagent for a conventionally used non-labeled reagent.

[0018] The compounds of the present application can exist as their pharmaceutically acceptable salts.

[0019] The term "pharmaceutically acceptable" means that the corresponding compound, carrier, or molecule is suitable for administration to humans. Preferably, this term refers to those approved for use in mammals (preferably humans) by regulatory agencies such as CFDA (China), EMEA (Europe), and FDA (USA).

[0020] Pharmaceutically acceptable salts include the acid addition salts and base addition salts thereof. Suitable acid addition salts are formed with acids which form non-toxic salts. Examples include acetate, adipate, aspartate, benzoate, benzenesulfonate, bicarbonate / carbonate, hydrogensulfate / sulfate, borate, camphorsulfonate, citrate, cyclohexanaminesulfonate, ethanedisulfonate, formate, fumarate, glucoheptonate, gluconate, glucuronate, hexafluorophosphate, 2-(4-hydroxybenzyl)benzoate, hydrogen chloride / chloride, hydrogen bromide / bromide, hydrogen iodide / iodide. Examples of suitable base addition salts include, but are not limited to, 2-hydroxyethylsulfonate, lactate, malate, maleate, malonate, methanesulfonate, methylsulfate, naphthalene, 2-naphthalenesulfonate, nicotinate, nitrate, lactate, oxalate, hexadecanoate, phosphate / hydrogenphosphate / dihydrogenphosphate, pyroglutamate, glucarate, stearate, salicylate, tannate, tartrate, toluenesulfonate, and trifluoroacetate. Suitable base addition salts are formed with alkalis that form non-toxic salts. Examples include, but are not limited to, aluminum, arginine, calcium, choline, diethylamine, diethanolamine, glycine, lysine, magnesium, meglumine, ethanolamine, potassium, sodium, tristearin, and zinc salts. Acid and alkali hemisalts, such as sulfate hemisalts and calcium hemisalts, can also be formed. For a description of suitable salts, see Handbook of Pharmaceutical Salts: Properties, Selection and Use by Stahl and Wermuth (Wiley-VCH, 2002). Methods for preparing pharmaceutically acceptable salts of the compounds described herein are known to those skilled in the art.

[0021] The compounds of the present application can also exist in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, ethanol, etc. The compounds may also exist in one or more crystalline states, i.e., polycrystalline forms, or they may exist as amorphous solids. All of these forms are included within the scope of the present application.

[0022] The present application further includes prodrugs of the compounds of the present application. The term "prodrug" refers to a derivative that is converted into the compounds of the present application in vivo under physiological conditions such as enzymes, gastric acid, etc., for example, by reactions such as oxidation, reduction, hydrolysis, etc., each of which is carried out under enzyme catalysis. Therefore, some derivatives of the compounds of the present application themselves have very little or no pharmacological activity, but can be converted into the compounds of the present application having the desired activity when administered to or in the body.

[0023] The present application further includes metabolites of the compounds of the present application. The term "metabolite" means any molecule derived in a cell or organism, preferably a human, from any compound of the present application.

[0024] As used herein, the term "substituted" means that one or more (preferably 1 to 5, more preferably 1 to 3) hydrogen atoms in a group are independently replaced with a corresponding number of substituents.

[0025] As used herein, the term "independent" means that when the number of substituents is more than one, the substituents may be the same or different.

[0026] As used herein, the terms "optionally" or "optionally" indicate that the event described thereby may or may not occur. For example, a group being "optionally substituted" means that the group may be unsubstituted or may be substituted.

[0027] The term "halogen" or "halo" means -F, -Cl, -Br, or -I.

[0028] As used herein, the term "alkyl" means a saturated aliphatic hydrocarbon, including straight and branched chains. In some embodiments, an alkyl group has 1 to 8, or 1 to 6, or 1 to 4, or 1 to 3 carbon atoms. For example, "C 1-8 The term "alkyl" means a straight or branched chain group of atoms having from 1 to 8 carbon atoms. 1-8 The term "alkyl" is defined as "C 1-6 Alkyl," "C 1-3 Examples of alkyl include, but are not limited to, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, 2-pentyl, 3-pentyl, isopentyl, neopentyl, (R)-2-methylbutyl, (S)-2-methylbutyl, 3-methylbutyl, 2,3-dimethylpropyl, 2,3-dimethylbutyl, hexyl, and the like. An alkyl group may be optionally substituted with one or more (e.g., 1 to 5) suitable substituents.

[0029] As used herein, the term "alkenyl" refers to an aliphatic hydrocarbon having at least one carbon-carbon double bond, including straight and branched chains having at least one carbon-carbon double bond. In some embodiments, alkenyl has 2 to 8 carbon atoms, 2 to 6 carbon atoms, 3 to 6 carbon atoms, or 2 to 4 carbon atoms. For example, "C 2-8 The term "alkenyl" refers to a straight or branched chain unsaturated group (having at least one carbon-carbon double bond) of 2 to 8 carbon atoms. The double bond may or may not be the point of attachment to another group. Alkenyl includes, but is not limited to, vinyl, 1-propenyl, 2-propenyl, 2-methyl-2-propenyl, butenyl, pentenyl, 3-hexenyl, and the like. An alkenyl group may be optionally substituted with one or more (e.g., 1 to 5) suitable substituents. When a compound of formula (I) contains an alkenyl group, the alkenyl group may exist in the pure E form, the pure Z form, or any mixture thereof.

[0030] As used herein, the term "alkynyl" refers to an aliphatic hydrocarbon having at least one carbon-carbon triple bond, including straight and branched chains having at least one carbon-carbon triple bond. In some embodiments, alkynyl groups have 2 to 8 carbon atoms, 2 to 6 carbon atoms, 3 to 6 carbon atoms, or 2 to 4 carbon atoms. For example, "C 2-8 The term "alkynyl" means a straight or branched chain unsaturated group (having at least one carbon-carbon triple bond) of 2 to 8 carbon atoms. The triple bond may or may not be the point of attachment to another group. Alkynyl includes, but is not limited to, ethynyl, 1-propynyl, 2-propynyl, 2-methyl-2-propynyl, butynyl, pentynyl, 3-hexynyl, and the like. An alkynyl group may be optionally substituted with one or more (e.g., 1 to 5) suitable substituents.

[0031] As used herein, "C 3-8 The term "alicyclic group" means an alicyclic group having 3 to 8 carbon atoms forming a ring. 3-7 The term "alicyclic group" means an alicyclic group having 3 to 7 carbon atoms forming a ring. 3-6 The term "alicyclic group" means an alicyclic group having 3 to 6 carbon atoms forming the ring. An alicyclic group may be a monocyclic ring. The definition of alicyclic group also includes unsaturated non-aromatic alicyclic groups. Examples of alicyclic groups are, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclohexenyl, cycloheptyl, cyclooctyl, cyclohexadienyl, cyclopentenyl, cycloheptenyl, and cyclooctenyl. An alicyclic group may be optionally substituted with one or more suitable substituents.

[0032] As used herein, "C 6-12 The term "dicycloaliphatic radical" refers to a bicyclic alicyclic radical having 6 to 12 carbon atoms forming the ring. Dicycloaliphatic radicals may be fused or may include bridged bicyclic alicyclic radical systems.

[0033] As used herein, "C 8―15 The term "three-membered tricycloaliphatic group" means an alicyclic group having 8 to 15 carbon atoms forming the ring and having three rings. Tricycloaliphatic groups may be fused or bridged.

[0034] As used herein, the term "n-membered heteroalicyclic group" refers to an alicyclic group having m ring-forming carbon atoms and (nm) ring-forming heteroatoms, where the heteroatoms are selected from O, S, and N. For example, the term "4- to 8-membered heteroalicyclic group" means that the substituents of the heteroalicyclic group contain a total of 4 to 8 ring atoms, at least one of which is a heteroatom. The term "4- to 6-membered heteroalicyclic group" means that the substituents of the heteroalicyclic group contain a total of 4 to 6 ring atoms, at least one of which is a heteroatom. The term "3- to 10-membered heteroalicyclic group" means that the substituents of the heteroalicyclic group contain a total of 3 to 10 ring atoms, at least one of which is a heteroatom. The term "n-membered dicycloheteroalkyl" refers to a dicycloheteroalkyl having m ring-forming carbon atoms and (nm) ring-forming heteroatoms, where the heteroatoms are selected from O, S, and N.Examples of heteroalicyclic groups include azetidine, thietane, dihydrofuran, dihydrothiophene, tetrahydrothiophene, tetrahydrofuranyl, tetrahydrotriazine, tetrahydropyrazolyl, tetrahydrooxazine, tetrahydropyrimidyl, octahydrobenzofuranyl, octahydrobenzimidazolyl, octahydrobenzothiazole, imidazolidinyl, pyrrolidinyl, piperidinyl, piperazinyl, oxazolidinyl, thiazolidinyl, pyrazolidinyl, thiomorpholinyl, tetrahydropyranyl, tetrahydrothiopyranyl, tetrahydrothiazinyl, tetrahydrothiadiazine, tetrahydrooxazolyl, morpholinyl, oxetanyl, tetrahydro Examples of heteroalicyclic groups include, but are not limited to, tetrahydrodioxazine, oxazin, oxathiazin, quinuclidinyl, chromanyl, isochromanyl, dihydrobenzodioxinyl, benzodioxolyl, benzoxazine, dihydroindolyl, dihydrobenzofuranyl, tetrahydroquinolyl, isochromyl, dihydro-1H-isoindolyl, 2-azadicyclo[2.2.1]heptanoyl, 3-azadicyclo[3.1.0]hexyl, 3-azadicyclo[4.1.0]heptyl, oxepan, thiepan, azepan, etc. Heteroalicyclic groups may be optionally substituted with one or more suitable substituents.

[0035] As used herein, "C 5-8 The term "aryl" refers to an aryl having 5 to 8 carbon atoms and having an aromatic ring, such as phenyl.

[0036] As used herein, the term "n-membered heteroaryl" refers to a heteroaryl having m carbon atoms forming an aromatic ring and (nm) heteroatoms forming the aromatic ring, wherein the heteroatoms are selected from O, S, and N. For example, 5- to 7-membered heteroaryls include, but are not limited to, furyl, thienyl, pyrrolyl, thiazolyl, pyrazolyl, imidazolyl, pyridyl, pyranyl, pyridazinyl, pyrimidinyl, and pyrazinyl. Heteroaryls may be optionally substituted with one or more suitable substituents.

[0037] As used herein, "C 7-11 The term "dicycloaryl" means a dicycloaryl having 7 to 11 carbon atoms, such as a naphthyl group, an indenyl group, etc. A dicycloaryl may be optionally substituted with one or more suitable substituents.

[0038] As used herein, the term "n-membered dicycloheteroaryl" refers to a dicycloheteroaryl having m carbon atoms forming an aromatic bicyclic ring and (nm) heteroatoms forming an aromatic bicyclic ring, wherein the heteroatoms are selected from O, S, and N. For example, 7- to 11-membered dicycloheteroaryls include, but are not limited to, quinoline, isoquinoline, indolyl, purine, benzothiazole groups, etc. Dicycloheteroaryls may be optionally substituted with one or more suitable substituents.

[0039] As used herein, the term "11- to 15-membered tricyclo" includes, but is not limited to, acridine, etc. The 11- to 15-membered tricyclo may be optionally substituted with one or more suitable substituents.

[0040] As used herein, the term "halogenated alkyl" refers to an alkyl group having one or more halogen substituents (at most a perhalogenated alkyl, i.e., every hydrogen atom of the alkyl group is replaced with a halogen atom). For example, "C 1-6The term "halogenated alkyl" refers to a C alkyl group having one or more halogen substituents. 1-6 It refers to an alkyl group (which may be at most a perhalogenated alkyl group, i.e., each hydrogen atom of the alkyl group is replaced with a halogen atom). Another example is "C 1-4 The term "halogenated alkyl" refers to a C alkyl group having one or more halogen substituents. 1-4 "C" means an alkyl group (which may be at most a perhalogenated alkyl group, i.e., each hydrogen atom of the alkyl group is replaced with a halogen atom). 1-3 The term "halogenated alkyl" refers to a C alkyl group having one or more halogen substituents. 1-3 "C" means an alkyl group (which may be at most a perhalogenated alkyl group, i.e., each hydrogen atom of the alkyl group is replaced with a halogen atom). 1-2 The term "halogenated alkyl" refers to a C alkyl group having one or more halogen substituents. 1-2 It refers to an alkyl group (i.e., methyl or ethyl) that is at most a perhalogenated alkyl, i.e., each hydrogen atom of the alkyl group is replaced with a halogen atom. By way of further example, the term "C1 halogenated alkyl" refers to a methyl group having one, two, or three halogen substituents. Examples of halogenated alkyl groups include CF3, C2F5, CHF2, CH2F, CH2CF3, CH2Cl, and the like.

[0041] As used herein, the term "alkoxy" refers to an alkyl bonded to an oxygen atom by a single bond. The bond between the alkoxy and the molecule is the oxygen atom. The alkoxy can be represented as alkyl-O-. 1-6 The term "alkoxy" means a straight or branched chain alkoxy group containing 1 to 6 carbon atoms. 1-6 The term "alkoxy" is defined as "C 1-3 The term "alkoxy" includes, but is not limited to, methoxy, ethoxy, propoxy, isopropoxy, butoxy, hexyloxy, and the like. An alkoxy may be optionally substituted with one or more suitable substituents.

[0042] As used herein, the term "3- to 14-membered ring" refers to a saturated or unsaturated ring system having 3 to 14 ring-forming atoms. Similarly, the term "3- to 6-membered ring" refers to a saturated or unsaturated ring system having 3 to 6 ring-forming atoms, and the term "3- to 8-membered ring" refers to a saturated or unsaturated ring system having 3 to 8 ring-forming atoms.

[0043] In this specification, numerical ranges relating to the number of substituents, the number of carbon atoms, and the number of ring atoms are equivalent to listing all integers within the range one by one, and the ranges are merely abbreviated notations. For example, "4 to 6-membered" indicates 4, 5, or 6-membered, "5 to 7-membered" indicates 5, 6, or 7-membered, "7 to 11-membered" indicates 7, 8, 9, 10, or 11-membered, "4 to 8-membered" indicates 4, 5, 6, 7, or 8-membered, "3 to 10-membered" indicates 3, 4, 5, 6, 7, 8, 9, or 10-membered, "3 to 14-membered" indicates 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, or 14-membered, and "C 1-3 " indicates one carbon atom (C1), two carbon atoms (C2) or three carbon atoms (C3), and "C 1-4 " indicates one carbon atom (C1), two carbon atoms (C2), three carbon atoms (C3) or four carbon atoms (C4), and "C 3-6 " indicates three carbon atoms (C3), four carbon atoms (C4), five carbon atoms (C5) or six carbon atoms (C6), and "C 3-8 " indicates 3 carbon atoms (C3), 4 carbon atoms (C4), 5 carbon atoms (C5), 6 carbon atoms (C6), 7 carbon atoms (C7) or 8 carbon atoms (C8), and "C 5-7 " indicates five carbon atoms (C5), six carbon atoms (C6) or seven carbon atoms (C7), and "C 7-11 " is a molecule with seven carbon atoms (C7), eight carbon atoms (C8), nine carbon atoms (C9), and ten carbon atoms (C 10 ) or 11 carbon atoms (C 11 ) and the like. Thus, numerical ranges relating to the number of substituents, the number of carbon atoms, and the number of ring atoms also include any one of the subranges, and each subrange is also considered to be disclosed herein.

[0044] In the above formula (I), R 1 are H, CN, and C 1-4 Alkyl, C 1-4 Alkyl halides, C 1-4 alkoxy, halogen.

[0045] In some embodiments, R 1 is H.

[0046] In some embodiments, R 1 is a halogen, e.g., R 1 is selected from F, Cl, Br, and I.

[0047] In some embodiments, R 1 is C 1-4 Alkyl or C 1-4 Alkyl halides include, for example, methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, optionally substituted with one or more halogen atoms (eg, fluorine, chlorine, bromine, iodine).

[0048] In some embodiments, R 1 is C 1-4 Alkoxy is, for example, methoxy, ethoxy, propoxy, isopropoxy, n-butoxy, isobutoxy.

[0049] In some embodiments, R 1 is CN.

[0050] What you need to understand is that any of the above R 1

[0033] The embodiment of

[0034] may be any of the R 2 , R 3 , R 5 , R 6 , X, L and n can be combined in any combination with the embodiments.

[0051] In the above formula (I), R 2 are H, CN, and C 1-4 Alkyl, C1-4 Alkyl halides, C 1-4 alkoxy, halogen.

[0052] In some embodiments, R 2 is H.

[0053] In some embodiments, R 2 is a halogen, for example, R 2 is selected from F, Cl, Br, and I.

[0054] In some embodiments, R 2 is C 1-4 Alkyl or C 1-4 Alkyl halides include, for example, methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, optionally substituted with one or more halogen atoms (eg, fluorine, chlorine, bromine, iodine).

[0055] In some embodiments, R 2 is C 1-4 Alkoxy, for example, methoxy, ethoxy, propoxy, isopropoxy, n-butoxy, isobutoxy.

[0056] In some embodiments, R 2 is CN.

[0057] What you need to understand is that any of the above R 2

[0033] The embodiment of

[0034] may be any of the R 1 , R 3 , R 5 , R 6 , X, L and n can be combined in any combination with the embodiments.

[0058] In some embodiments, R 1 and R 2 may be the same. For example, R 1 and R 2 are all halogen, for example Cl or F, and further, for example, R 1 and R2 are both H. In one preferred embodiment, R 1 and R 2 are both Cl. In one preferred embodiment, R 1 and R 2 are both H.

[0059] In some other embodiments, R 1 and R 2 may be different.

[0060] In the above formula (I), R 3 is H, C 1-4 Alkyl, C 1-4 Alkyl halides, C 1-4 Alkoxy, C 3-6 It is selected from an alicyclic group and a 4- to 6-membered heteroalicyclic group.

[0061] In some embodiments, R 3 is H.

[0062] In some embodiments, R 3 is C 1-3 Alkyl or C 1-3 Alkyl halides, such as R 3 is selected from methyl, ethyl, propyl, isopropyl, optionally substituted with one or more halogen atoms (eg, fluorine, chlorine, bromine, iodine).

[0063] In some embodiments, R 3 is C 1-3 Alkoxy, for example, R 3 is selected from methoxy, ethoxy, propoxy, isopropoxy.

[0064] In some embodiments, R 3 is C 3-6 It is an alicyclic group, for example, R 3 is selected from cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclohexenyl, cyclohexadienyl, cyclopentenyl.

[0065] In some embodiments, R 3 is a 4-6 membered heteroalicyclic group, and the heteroatoms can be selected from O, S, and N. For example, R 3 is selected from oxetanyl, thietane, azetidine, tetrahydrofuranyl, tetrahydrothienyl, pyrrolidinyl, tetrahydropyranyl, tetrahydrothiopyranyl, piperidinyl, morpholinyl, piperazinyl.

[0066] In some embodiments, R 3 is methyl.

[0067] What you need to understand is that any of the above R 3

[0033] The embodiment of

[0034] may be any of the R 1 , R 2 , R 5 , R 6 , X, L and n can be combined in any combination with the embodiments.

[0068] In the above formula (I), R 6 The number of R may be 1, 2 or 3, 6 is H, halogen, -CN, -OH, -NO2, -NR 7 R 8 , C 1-4 Alkyl, C 1-4 Alkyl halides, C 1-4 Alkoxy, C 3-6 are independently selected from an alicyclic group and a 4- to 6-membered heteroalicyclic group (heteroatoms can be selected from O, S, and N), where R 7 and R 8 is H, C 1-6 Alkyl, C 1-4 Alkyl halides, C 1-4 alkoxy, or R 7 and R 8 form a 3- to 6-membered ring together with the N atom to which they are connected, and in this case, for example, R 7 , R 8 are both (CH2) n(n=1, 2, 3, 4, 5, etc.) or R 7 and R 8 together with the N atom to which they are connected form a 5- or 6-membered N-containing aromatic ring.

[0069] In some preferred embodiments, R 6 is H.

[0070] In some embodiments, R 6 is a halogen, for example, R 6 is selected from F, Cl, Br, and I.

[0071] In some embodiments, R 6 are -CN, -OH, and -NO2.

[0072] In some embodiments, R 6 is C 1-3 Alkyl or C 1-3 Alkyl halides, such as R 6 is selected from methyl, ethyl, propyl, isopropyl, optionally substituted with one or more halogen atoms (eg, fluorine, chlorine, bromine, iodine).

[0073] In some embodiments, R 6 is C 1-3 Alkoxy, for example, R 6 is selected from methoxy, ethoxy, propoxy, isopropoxy.

[0074] In some embodiments, R 6 is C 3-6 It is an alicyclic group, for example, R 6 is selected from cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclohexenyl.

[0075] In some embodiments, R 6 is a 4-6 membered heteroalicyclic group (heteroatoms can be selected from O, S and N), such as R 6is selected from oxetanyl, thietane, azetidine, tetrahydrofuranyl, tetrahydrothienyl, pyrrolidinyl, tetrahydropyranyl, tetrahydrothiopyranyl, piperidinyl, morpholinyl, piperazinyl.

[0076] In some embodiments, R 6 is -NR 7 R 8 where R 7 and R 8 is H, C 1-3 Alkyl (e.g., methyl, ethyl, propyl, isopropyl, etc.), C 1-3 halogenated alkyl (e.g., methyl, ethyl, propyl, isopropyl substituted with one or more halogen atoms selected from fluorine, chlorine, bromine, iodine), C 1-3 alkoxy (e.g., methoxy, ethoxy, propoxy, isopropoxy), or R 7 and R 8 form a 3- to 6-membered ring together with the N atom to which they are connected, for example, in this case, R 7 , R 8 are both (CH2) n (n=1, 2, 3, 4, 5, etc.) or R 7 and R 8 together with the N atom to which they are linked form a 5- or 6-membered N-containing aromatic ring, for example, pyrrole, pyridine, pyrimidine, imidazole, pyrazole, pyrrolidine, hexahydropyridine, etc.

[0077] In some embodiments, R 6 is dimethylamino, diethylamino, methylethylamino.

[0078] In some preferred embodiments, R 6 is one.

[0079] What you need to understand is that any of the above R 6

[0033] The embodiment of

[0034] may be any of the R 1 , R 2 , R3 , R 5 , X, L and n can be combined in any combination with the embodiments.

[0080] In the above formula (I), X is a connecting bond, O, S, or (NR 4 ), where R 4 is H, C 1-4 Alkyl, C 1-4 Alkyl halides, C 1-4 Alkoxy, C 3-6 It is selected from alicyclic groups and 4- to 6-membered heteroalicyclic groups (heteroatoms can be selected from O, S and N).

[0081] In some embodiments, X is a linking bond (ie, X is absent, and the chemical groups on either side of X are then directly linked).

[0082] In some embodiments, X is —O—.

[0083] In some embodiments, X is -S-.

[0084] In some embodiments, X is imino, i.e., —(NH)—.

[0085] In some embodiments, X is —(N(CH 3 ))—.

[0086] In some embodiments, X is selected from a linking bond, —O—, —(NH)—.

[0087] It should be understood that any of the above embodiments of X may be combined with any of the R 1 , R 2 , R 3 , R 5 , R 6 , L and n can be combined in any combination with the embodiments.

[0088] In the above formula (I), n is 1, 2 or 3.

[0089] In some embodiments, n is 1.

[0090] In some embodiments, n is 2.

[0091] In some embodiments, n is 3.

[0092] It should be understood that any of the above n embodiments may be combined with any of the R 1 , R 2 , R 3 , R 5 , R 6 , L and X embodiments can be combined in any way.

[0093] In the above formula (I), L is (C=O), (O=S=O), CR a R b or a connecting bond, where R a and R b is H, C 1-4 Alkyl, C 1-4 Alkyl halides, C 1-4 Alkoxy, C 3-6 are independently selected from an alicyclic group and a 4- to 6-membered heteroalicyclic group (heteroatoms can be selected from O, S, and N), or R a and R b form a 3- to 6-membered ring together with the carbon atoms connected thereto, for example, in this case, R a , R b are both (CH2) n (n=1, 2, 3, 4, 5, etc.) and form a 3- to 6-membered saturated alicyclic ring, or R a and R b form a 3- to 6-membered unsaturated alicyclic ring or a 6-membered aromatic ring together with the C atom connected thereto.

[0094] In some embodiments, L is —(C═O)—.

[0095] In some embodiments, L is -(O=S=O)-.

[0096] In some embodiments, L is a connecting bond (L is absent, in which case R 5 connects directly to N).

[0097] In some embodiments, L is -(CR a R b )-, where R a and R b is H, C 1-4 Alkyl (e.g., methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, tert-butyl, etc.), C 1-4 halogenated alkyl (e.g., methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, tert-butyl, etc., substituted with one or more halogen atoms selected from fluorine, chlorine, bromine, iodine); C 1-4 Alkoxy (e.g., methoxy, ethoxy, propoxy, isopropoxy, n-butoxy, isobutoxy, tert-butoxy groups), C 3-6 are each independently selected from alicyclic groups (e.g., cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclohexenyl, cyclohexadienyl, cyclopentenyl, etc.) and 4- to 6-membered heteroalicyclic groups (e.g., oxetanyl, thietane, azetidine, tetrahydrofuranyl, tetrahydrothienyl, pyrrolidinyl, tetrahydropyranyl, tetrahydrothiopyranyl, piperidinyl, morpholinyl, piperazinyl, etc.), or R a and R b form a 3- to 6-membered ring (preferably a 3- to 6-membered alicyclic ring) together with the carbon atoms connected thereto. For example, L may be -CH2-, -C(CH3)2-, -CH(CH3)-, cyclopropylene, cyclobutylene, cyclopentylene, cyclohexylene, phenylene, etc.

[0098] It should be understood that any of the above L embodiments may be combined with any of the R embodiments described above and below. 1 , R 2 , R 3 , R 5 , R 6 , n, and X can be combined in any manner.

[0099] In the above formula (I), R 5 may be any substituent commonly used in organic chemistry and is not particularly limited.

[0100] In some embodiments, R 5 is C 1-6 alkyl, e.g., R 5 is selected from methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, t-butyl, pentyl, 2-pentyl, 3-pentyl, isopentyl, neopentyl, (R)-2-methylbutyl, (S)-2-methylbutyl, 3-methylbutyl, 2,3-dimethylpropyl, 2,3-dimethylbutyl, hexyl.

[0101] In some embodiments, R 5 is C 3-7 It is an alicyclic group, for example, R 5 is selected from cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclohexenyl, cycloheptyl, cyclohexadienyl, cyclopentenyl, cycloheptenyl.

[0102] In some embodiments, R 5 is a 3- to 10-membered heteroalicyclic group, for example, R 5Examples include azetidine, thietane, dihydrofuran, dihydrothiophene, tetrahydrothiophene, tetrahydrofuranyl, tetrahydrotriazine, tetrahydropyrazolyl, tetrahydrooxazine, tetrahydropyrimidyl, octahydrobenzofuranyl, octahydrobenzimidazolyl, octahydrobenzothiazole, imidazolidinyl, pyrrolidinyl, piperidinyl, piperazinyl, oxazolidinyl, thiazolidinyl, pyrazolidinyl, thiomorpholinyl, tetrahydropyranyl, tetrahydropyranyl, tetrahydrotriazine, tetrahydropyrazolyl, tetrahydrooxazine, tetrahydropyrimidyl, octahydrobenzofuranyl, octahydrobenzimidazolyl, octahydrobenzothiazole, imidazolidinyl, pyrrolidinyl, piperidinyl, piperazinyl, oxazolidinyl, thiazolidinyl, pyrazolidinyl, thiomorpholinyl, tetrahydropyranyl, tetrahydrotriazine, tetrahydrotriazinyl ... and selected from tetrahydrothiopyranyl, tetrahydrothiazinyl, tetrahydrothiadiazine, tetrahydrooxazolyl, morpholinyl, oxetanyl, tetrahydrodioxazine, oxazine, oxathiazine, quinuclidinyl, chromanyl, isochromanyl, dihydrobenzodioxanyl, benzodioxole, benzoxazine, dihydroindolyl, dihydrobenzofuranyl, tetrahydroquinolyl, isochroman, dihydro-1H-isoindolyl, oxepane, thiepane, and azepane.

[0103] In some embodiments, R 5 is H, halogen, —OH, —NO2, —CN, —SF5 or —SH.

[0104] In some embodiments, R 5 -SC 1-4 Alkyl, C 1-6 Alkyl, C 1-6 Alkyl halides, C 1-6 Alkoxy, C 1-6 Halogenated alkoxy, C 2-8 Alkenyl, C 2-8 alkynyl.

[0105] In some embodiments, R 5 is C 5-8 Aryl (e.g., phenyl), 5- to 10-membered heteroaryl (e.g., furyl, thienyl, pyrrolyl, thiazolyl, pyrazolyl, imidazolyl, pyridyl, pyranyl, pyridazinyl, pyrimidinyl, pyrazinyl, etc.), -C 1-4 Alkyl-(C 5-8 aryl), -C1-4 alkyl-(5-10 membered heteroaryl).

[0106] In some embodiments, R 5 is -N(R 10 )(R 11 ), -N(R 10 )(C(=O)R 11 ), -N(R 10 )(C(=O)-OR 11 ), -N(R 12 )(C(=O)-N(R 10 )(R 11 )), -C(=O)-N(R 10 )(R 11 ), -C(=O)-R 12 , -C(=O)-OR 12 , -OC(=O)R 12 , -N(R 10 )(S(=O)2R 11 ), -S(=O)2-N(R 10 )(R 11 ), -SR 12 and -OR 12 where R 10 , R 11 and R 12 For each occurrence, H, C 1-6 Alkyl, C 1-6 Alkyl halides, C 3-7 Alicyclic group, 3- to 10-membered heteroalicyclic group, C 5-8 Aryl, 5-7 membered heteroaryl, C 7-11 Dicycloaryl, 7-11 membered dicycloheteroaryl, -C 1-4 Alkyl-(C 3-7 alicyclic group), -C 1-4 Alkyl-(3- to 10-membered heteroalicyclic group), -C 1-4 Alkyl-(C 6-12 Dicycloaliphatic group), -C 1-4 Alkyl-(6- to 12-membered dicycloaliphatic group), -C 1-4 Alkyl-(C 8-15 -membered tricycloaliphatic group), -C 1-4 Alkyl-(8- to 15-membered tricycloaliphatic group), -C 1-4 Alkyl-(C 5-8aryl) and -C 1-4 alkyl-(5-10 membered heteroaryl), where each option within the group is selected from halogen, —OH, —NH2, —NH(CH3), —N(CH3)2, —CN, —NO2, —SF5, —SH, —SC 1-4 Alkyl, oxo, C 1-4 Alkyl, C 2-6 Alkenyl, C 2-6 Alkynyl, C 3-7 Alicyclic group, 3- to 10-membered heteroalicyclic group, C 5-8 Aryl, 5-7 membered heteroaryl, C 7-11 Dicycloaryl, 7-11 membered dicycloheteroaryl, C 1-4 Hydroxyalkyl, -SC 1-4 Alkyl, -C(=O)H, -C(=O)-C 1-4 Alkyl, -C(=O)-OC 1-4 Alkyl, -C(=O)-NH2, -C(=O)-N(C 1-4 Alkyl)2, C 1-4 Alkyl halides, C 1-4 Alkoxy and C 1-4 optionally substituted with 0, 1, 2, 3, or 4 substituents each independently selected from the group consisting of halogenated alkoxy, or R 10 and R 11 form a 3- to 14-membered ring together with the atoms to which they are attached (e.g., R 10 , R 11 are both (CH2) n (n=1, 2, 3, 4, 5, 6, 7, 8, etc.) or R 10 and R 11 together with the atoms to which they are connected form a 5- to 14-membered aromatic ring).

[0107] Above R 5 It should be understood that any of the exemplary groups listed above may be optionally substituted. 5 Any group listed in may optionally be 0, 1, 2, 3 or 4 R 5a may be substituted with R 5a are halogens, -OH, -NO2, -CN, -SF5, -SH, -SC1-4 Alkyl, oxo, C 1-4 Alkyl, C 1-4 Alkyl halides, C 1-4 Alkoxy, C 1-4 Halogenated alkoxy, C 2-8 Alkenyl, C 2-8 Alkynyl, C 3-7 Alicyclic group, 3- to 10-membered heteroalicyclic group, C 5-8 Aryl, 5- to 7-membered heteroaryl, -N(R 13 )(R 14 ), -N(R 13 )(C(=O)R 14 ), -N(R 13 )(C(=O)-OR 14 ), -N(R 15 )(C(=O)-N(R 13 )(R 14 )), -C(=O)-N(R 13 )(R 14 ), -C(=O)-R 15 , -C(=O)-OR 15 , -OC(=O)R 15 , -N(R 13 )(S(=O)2R 14 ), -S(=O)2-N(R 13 )(R 14 ), -SR 15 and -OR 15 are independently selected from, where R 13 , R 14 and R 15 For each occurrence, H, C 1-6 Alkyl, C 1-6 Alkyl halides, C 3-7 Alicyclic group, 3- to 10-membered heteroalicyclic group, C 5-8 Aryl, 5-7 membered heteroaryl, C 7-11 Dicycloaryl, 7-11 membered dicycloheteroaryl, -C 1-4 Alkyl-(C 3-7 alicyclic group), -C 1-4 Alkyl-(3- to 10-membered heteroalicyclic group), -C 1-4 Alkyl-(C 6-12 Dicycloaliphatic group), -C 1-4 Alkyl-(6- to 12-membered dicycloaliphatic group), -C 1-4Alkyl-(C 8-15 -membered tricycloaliphatic group), -C 1-4 Alkyl-(8- to 15-membered tricycloaliphatic group), -C 1-4 Alkyl-(C 5-8 aryl) and -C 1-4 alkyl-(5-10 membered heteroaryl), where each option within the group is selected from halogen, —OH, —NH2, —NH(CH3), —N(CH3)2, —CN, —NO2, —SF5, —SH, —SC 1-4 Alkyl, oxo, C 1-4 Alkyl, C 2-6 Alkenyl, C 2-6 Alkynyl, C 3-7 Alicyclic group, 3- to 10-membered heteroalicyclic group, C 5-8 Aryl, 5-7 membered heteroaryl, C 7-11 Dicycloaryl, 7-11 membered dicycloheteroaryl, C 1-4 Hydroxyalkyl, -SC 1-4 Alkyl, -C(=O)H, -C(=O)-C 1-4 Alkyl, -C(=O)-OC 1-4 Alkyl, -C(=O)-NH2, -C(=O)-N(C 1-4 Alkyl)2, C 1-4 Alkyl halides, C 1-4 Alkoxy and C 1-4 optionally substituted with 0, 1, 2, 3, or 4 substituents each independently selected from the group consisting of halogenated alkoxy, or R 13 and R 14 form a 3- to 14-membered ring together with the atoms to which they are attached (e.g., R 13 , R 14 are both (CH2) n (n=1, 2, 3, 4, 5, 6, 7, 8, etc.) or R 13 and R 14 together with the atoms to which they are connected form a 5- to 14-membered aromatic ring).

[0108] In some preferred embodiments, R 5is selected from methyl, ethyl, propyl, isopropyl, and cyclobutyl. Methyl, ethyl, propyl, isopropyl, or cyclobutyl may be optionally substituted with 0, 1, or 2 substituents each independently selected from the group consisting of methyl, ethyl, cyclopentyl, cyclopropyl, -CN, -OH, morpholinyl, and piperidinyl, which may be optionally substituted with 0, 1, or 2 substituents each independently selected from the group consisting of methyl, ethyl, cyclopentyl, cyclopropyl, -CN, and -OH. For example, in some preferred embodiments, R 5 is selected from (1-hydroxycyclopropyl)ethyl, 3-hydroxycyclobutyl, 2-cyanoethyl, 2-hydroxyethyl, 2-cyano-1-cyclopentylethyl, 1-cyanopropane, 2-morpholinoethyl, ethyl, and (1-methylpiperidin-4-yl)methyl.

[0109] In some preferred embodiments, R 5 is selected from halogens, for example F.

[0110] In some preferred embodiments, R 5 is selected from piperazinyl, morpholinyl, pyrrolidinyl, piperidinyl, and azetidine, which may be optionally substituted with 0, 1, or 2 substituents each independently selected from the group consisting of methyl, ethyl, cyclopentyl, cyclopropyl, —CN, and —OH. 5 is selected from methyl, ethyl, propyl, isopropyl, and cyclobutyl, wherein methyl, ethyl, propyl, isopropyl, or cyclobutyl is optionally substituted with 0, 1, or 2 substituents each independently selected from the group consisting of methyl, ethyl, cyclopentyl, cyclopropyl, —CN, —OH, morpholinyl, and piperidinyl, which substituents are optionally further substituted with 0, 1, or 2 substituents each independently selected from the group consisting of methyl, ethyl, cyclopentyl, cyclopropyl, —CN, and —OH.

[0111] In some preferred embodiments, R 5 is selected from piperazinyl, morpholinyl, pyrrolidinyl, piperidinyl, and azetidine. For example, in some preferred embodiments, R 5 is selected from 3,5-dimethylpiperazinyl, morpholinyl, 3-hydroxypyrrolidinyl, 4-methylpiperazinyl, 4-ethylpiperazinyl, 4-hydroxypiperidinyl, 1-methylpiperidinyl, 1-ethylpiperidin-4-yl, 1-methylazetidin-3-yl.

[0112] In some preferred embodiments, R 5 is selected from H, methyl, ethyl, n-propyl, isopropyl, butyl, methoxy, ethoxy, hydroxy, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, morpholinomethyl, hydroxycyclobutyl, hydroxycyclohexyl, cyanoethoxy, cyanomethyl, pyridin-3-yl, 1-methyl-1H-pyrazol-4-yl, 1-methyl-1H-pyrazol-3-yl, 4-methylpiperazin-1-yl, 1-methylpiperidin-4-yl, morpholinyl, pyrrolidin-3-yl, 3-hydroxypyrrolidin-1-yl, 3-cyanopyrrolidin-1-yl.

[0113] What you need to understand is that any of the above R 5

[0033] The embodiment of

[0034] may be any of the R 1 , R 2 , R 3 , R 6 , X, L and n can be combined in any combination with the embodiments.

[0114] In some preferred embodiments, the present application further relates to compounds of formula (Ia):

[0115] [ka] In the formula, X is —O— or —(NH)—, and L, n, R 3 , R 5, R 6 The definitions and preferred options of are the same as those for the compounds of formula (I).

[0116] In some preferred embodiments, the present application further relates to compounds represented by formula (Ib):

[0117] [ka] In the formula, L, n, R 5 , R 6 The definitions and preferred options of are the same as those for the compounds of formula (I).

[0118] In some preferred embodiments, the present application further relates to compounds represented by formula (Ic):

[0119] [ka] In the formula, X is —O— or —(NH)—, n, R 3 , R 5 , R 6 , R a , R b The definitions and preferred options of are the same as those for the compounds of formula (I).

[0120] In some preferred embodiments, the present application further relates to compounds represented by formula (IIa):

[0121] [ka] In the formula, n, R 5 , R 6 , R a , R b The definitions and preferred options of are the same as those for the compounds of formula (I).

[0122] In some preferred embodiments, the present application further relates to compounds represented by formula (IIb):

[0123] [ka] In the formula, n, R 5 , R 6 , R a , R b The definitions and preferred options of are the same as those for the compounds of formula (I).

[0124] In some more preferred embodiments, the compound of the present application is selected from the specific compounds set forth in each example of the present application.

[0125] The compounds of the present application can be synthesized by a person skilled in the art using conventional organic synthesis methods based on the specific structure of the compound. For example, the compound of formula (I) can be produced by the method shown in the following synthetic route (I) or (II).

[0126] Synthetic Route (I) [ka]

[0127] For example, the compound of formula (I) can be prepared by the method shown in the above synthetic route (I). G in intermediate Int-2 is selected from halogen, hydroxy, methanesulfonyl (OMs), p-toluenesulfonyl (OTs), etc. PG1, PG2, and PG3 are amino-protecting groups, such as tetrahydropyran (THP), benzyl (Bn), p-methoxybenzyl (PMB), SEM, Boc, etc. When G is halogen, OMs, or OTs, intermediates Int-1 and Int-2 can be subjected to an SN2 coupling reaction under alkaline conditions to produce intermediate Int-3. When G is OH, intermediate Int-3 can be obtained by a Mitsunobu reaction between Int-1 and Int-2. Condensation reaction of intermediate Int-3 with intermediate Int-4 produces compound Int-5. Under oxidation conditions (such as, but not limited to, Swern oxidation or IBX oxidation), Int-5 is converted to intermediate Int-6. By conversion of the protecting group, intermediate Int-6 can be converted to Int-8. Int-8 can be reacted with an appropriate starting material to further remove the protecting group under typical amide-forming reaction conditions (e.g., in the presence of DIPEA / HATU), sulfonamide-forming reaction conditions, urea-forming reaction conditions, SN2 coupling reaction conditions, or carbamate-forming reaction conditions to obtain the target compound of formula (I).

[0128] Synthetic Route (II) [ka]

[0129] Alternatively, compound Int-7 can be prepared by the method shown in synthetic pathway (II) above. Compound Int-9 is produced by condensation reaction between intermediate Int-1 and intermediate Int-4. Under oxidation conditions (e.g., but not limited to, Swern oxidation or IBX oxidation), Int-9 is converted to intermediate Int-10. Intermediate Int-6 can be converted to Int-11 by conversion of the protecting group. PG1, PG2, and PG3 are amino-protecting groups, such as, but not limited to, tetrahydropyran (THP), benzyl (Bn), p-methoxybenzyl (PMB), SEM, Boc, etc. G in intermediate Int-2 is selected from halogen, hydroxyl, methanesulfonyl (OMs), p-toluenesulfonyl (OTs), etc. When G is halogen, OMs, or OTs, intermediates Int-11 and Int-2 can be subjected to an SN2 coupling reaction under alkaline conditions to produce intermediate Int-7. When G is OH, intermediate Int-7 can be obtained by Mitsunobu reaction of Int-11 with Int-2.

[0130] Furthermore, those skilled in the art can obtain methods for synthesizing other compounds by appropriately adjusting the reaction raw materials and reaction conditions with reference to the synthetic routes for the specific compounds in the specific examples of the present application.

[0131] The compounds of the present application have been proven to have TRK kinase inhibitory activity, can effectively inhibit TRKA, TRKB, and / or TRKC, and have inhibitory activity or selectivity comparable to or greater than that of larotrectinib or entrectinib, thereby providing many options and possibilities for the development of broad-spectrum anticancer drugs, analgesics, anti-inflammatory drugs, etc.

[0132] In a second aspect, the present application provides pharmaceutical compositions, which comprise the aforementioned compounds of the present application, or isotopically labeled compounds thereof, or optical isomers, geometric isomers, tautomers or isomeric mixtures thereof, or pharmaceutically acceptable salts thereof, prodrugs thereof, or metabolites thereof, and one or more pharmaceutically acceptable carriers, adjuvants or excipients.

[0133] The pharmaceutical compositions of the present application can be prepared by methods well known in the pharmaceutical art and can be administered in a variety of ways, depending on the desired local or systemic treatment and on the area to be treated. Administration can be topical (including ophthalmic and mucosal administration, including intranasal, vaginal, and rectal), pulmonary (e.g., by inhalation or insufflation of powders or aerosols, including nebulizer administration, intratracheal, intranasal, epidermal, and transdermal), ophthalmic, oral, or parenteral. Ophthalmic administration methods can include topical administration (eye drops), subconjunctival, periocular, or intravitreal injection, or by a balloon catheter or ophthalmic insert surgically placed in the conjunctival sac. Parenteral administration can include intravenous, intraarterial, subcutaneous, intraperitoneal, or intramuscular injection or infusion, or intracranial (e.g., intrasheath or intracerebral) administration. Parenteral administration can be in the form of a single injection dose or can be achieved, for example, by a continuous infusion pump. Pharmaceutical compositions and formulations for topical administration may include transdermal patches, plasters, lotions, creams, gels, drops, suppositories, sprays, liquids and powders.

[0134] When a solid carrier is used, the formulation may be in the form of a tablet, placed in a hard capsule in powder or granular form, or in the form of a troche or lozenge. Solid carriers may contain conventional excipients such as binders, fillers, tableting lubricants, disintegrants, wetting agents, and the like. If necessary, tablets may be film-coated by conventional techniques. When a liquid carrier is used, the formulation may be in the form of a syrup, emulsion, ointment, softgel capsule, sterile injectable carrier, aqueous or non-aqueous liquid suspension, or a dry product that can be reconstituted with water or other suitable carrier before use. Liquid formulations may contain conventional additives such as suspending agents, emulsifiers, wetting agents, non-aqueous carriers (including edible oils), preservatives, and flavorings and / or colorants. For parenteral administration, the carrier usually comprises at least mostly sterile water, although saline, glucose solution, and the like may also be used. Injectable suspensions may also be used, in which case conventional suspending agents may be used. Conventional preservatives, buffers, and the like may also be added to parenteral dosage forms. Pharmaceutical compositions are prepared by conventional techniques appropriate to the desired formulation containing appropriate amounts of the active ingredient (ie, the compound of the present application).

[0135] Compositions suitable for parenteral injection may include physiologically acceptable sterile aqueous or non-aqueous solutions, dispersions, suspensions, or emulsions, as well as sterile powders for sterile injectable solutions or dispersions. Examples of suitable aqueous and non-aqueous carriers, diluents, and solvents include water, ethanol, polyols (propylene glycol, polyethylene glycol, glycerol, and the like), suitable mixtures thereof, vegetable oils (e.g., olive oil), and injectable organic esters (e.g., ethyl oleate).

[0136] These compositions may further contain various excipients, such as preservatives, wetting agents, emulsifying agents, and dispersing agents. To ensure the prevention of microbial action, various antibacterial and antifungal agents (e.g., parabens, chlorobutanol, phenol, sorbic acid, etc.) may be used. Furthermore, isotonic agents, such as sugars, sodium chloride, etc., may be included. Absorption of injectable pharmaceutical forms can be delayed by the use of agents delaying absorption (e.g., aluminum monostearate and gelatin).

[0137] Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound is mixed with at least one inert excipient (or carrier) (e.g., sodium citrate or dicalcium phosphate), which may further include: (a) fillers or fillers (e.g., starch, lactose, sucrose, glucose, mannitol, and silicic acid), (b) binders (e.g., carboxymethylcellulose, alginate esters, gelatin, polyvinylpyrrolidone, sucrose, and gum arabic), (c) humectants (e.g., glycerol), (d) disintegrating agents (e.g., agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain synthetic silicates, sodium carbonate), (e) solution blocking agents (e.g., paraffin), (f) absorption accelerators (e.g., quaternary ammonium compounds), (I) wetting agents (e.g., cetyl alcohol and glycerol monostearate), (h) adsorbents (e.g., kaolin and bentonite), and (i) lubricants (e.g., talc, calcium stearate, magnesium stearate, solid polyethylene glycol, sodium lauryl sulfate) or mixtures thereof.

[0138] Solid compositions of a similar type may also be employed as fillers in soft-filled and hard-filled gel capsules using, for example, lactose as well as high molecular weight polyethylene glycols and other excipients.

[0139] Solid dosage forms (e.g., tablets, dragees, capsules, pills, and granules) can be prepared with coatings and shells (e.g., enteric coatings and others known in the art). They may contain light-blocking agents, and may further comprise an active compound or compositions of various active compounds that release the active compound or compounds in a delayed manner in a specific part of the intestinal tract. Examples of coating compositions that can be used are polymers and waxes. The active ingredient may also be in microencapsulated form, which may, if appropriate, contain one or more of the above-mentioned excipients.

[0140] Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, solutions, dispersions, syrups, and elixirs. In addition to the active compound, the liquid dosage forms may contain inert diluents commonly used in the art (e.g., water or other solvents), solubilizing agents and emulsifiers (e.g., ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butanediol, dimethylformamide), oils (specifically, cottonseed oil, peanut oil, corn oil, olive oil, castor oil, sesame oil), glycerin, tetrahydrofuranol, polyethylene glycol, and fatty acid esters of sorbitan, or mixtures of these substances.

[0141] In addition to these inert diluents, the composition may further contain, for example, wetting agents, emulsifying and suspending agents, flavorings, seasonings, and aromatics.

[0142] In addition to the active compound, suspensions may contain suspending agents such as ethoxylated isooctadecanol, polyoxylated ethylene sorbitol, sorbitan esters, microcrystalline fibers, aluminum metahydroxide, bentonite, agar-agar, and Astragalus gel, or mixtures of these substances.

[0143] Dosage forms for topical administration of the compounds of the present application include ointments, powders, sprays, and inhalants. The active ingredient is mixed under sterile conditions with a physiologically acceptable carrier and any necessary preservatives, buffers, or propellants. Ophthalmic formulations, eye ointments, powders, and solutions are also included within the scope of this application.

[0144] The amount of the compound of the present application in pharmaceutical compositions and dosage forms can be appropriately determined by those skilled in the art as needed; for example, the compound of the present application may be present in the pharmaceutical composition or dosage form in a therapeutically effective amount.

[0145] In a third aspect, the present application relates to the use of a compound of the present application, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or isomeric mixture thereof, or a pharmaceutically acceptable salt thereof, a prodrug thereof, or a metabolite thereof, or a pharmaceutical composition as described above, in the manufacture of a medicament for treating a disease or condition associated with a TRK kinase or NTRK gene.

[0146] The present application further provides a method for treating a disease or condition associated with a TRK kinase or NTRK gene, the method comprising administering to a patient in need thereof a therapeutically effective amount of a compound of the present application, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer, or isomeric mixture thereof, or a pharmaceutically acceptable salt thereof, a prodrug thereof, or a metabolite thereof, or the pharmaceutical composition described above. The patient is preferably a mammal, more preferably a human patient. The route of administration may be oral, topical (including, but not limited to, external application, spray, etc.), parenteral gastrointestinal (including subcutaneous, intramuscular, cortical, and intravenous), bronchial, or intranasal administration.

[0147] Diseases or conditions associated with TRK kinase or NTRK genes include, but are not limited to, diseases caused by impaired TRK kinase activity and / or associated with fusion with NTRK genes, such as cancer, pain, inflammation, neurodegenerative diseases, and cell proliferation disorders. The compounds of the present application can be used, for example, to inhibit cancer cell proliferation, metastasis, and the like, to alleviate pain, and can be used for anti-inflammatory purposes and to treat or alleviate neurodegenerative diseases, etc.

[0148] In some embodiments, the disease or condition associated with a TRK kinase or NTRK gene is cancer.

[0149] Exemplary cancers include bladder cancer, breast cancer, cervical cancer, colorectal cancer, small intestine cancer, colon cancer, rectal cancer, anal cancer, endometrial cancer, head and neck cancer (e.g., cancer of the throat, larynx, nasopharynx, oropharynx, lip and oral cavity), kidney cancer, liver cancer (e.g., hepatocellular carcinoma, cholangiocarcinoma), lung cancer (e.g., adenocarcinoma, small cell and non-small cell lung cancer, small cell and non-small cell carcinoma, bronchial carcinoma, bronchial adenocarcinoma, pleuropulmonary blastoma), ovarian cancer, prostate cancer, testicular cancer, uterine cancer, esophageal cancer, gallbladder cancer, pancreatic cancer (e.g., exocrine pancreatic cancer), thyroid cancer, parathyroid cancer, skin cancer (e.g., squamous cell carcinoma, Kaposi's sarcoma, Merkel cell skin cancer), and brain cancer (e.g., astrocytoma, neural germ cell tumor, subependymoma, neuroectodermal tumor, pineal tumor).

[0150] Other exemplary cancers include hematopoietic malignancies such as leukemia or lymphoma, multiple myeloma, chronic lymphocytic lymphoma, adult T-cell leukemia, B-cell lymphoma, cutaneous T-cell lymphoma, acute myeloid leukemia, Hodgkin's or non-Hodgkin's lymphoma, myeloproliferative neoplasms (e.g., polycythemia vera, essential thrombocytosis, and essential myelofibrosis), Wattesazone macroglobulinemia, hairy cell lymphoma, chronic myeloid lymphoma, acute lymphoblastic lymphoma, AIDS-related lymphoma, and Burkitt's lymphoma.

[0151] Further exemplary cancers include eye tumors, glioblastoma, melanoma, rhabdomyosarcoma, lymphosarcoma, and osteosarcoma.

[0152] In some preferred embodiments, the disease or condition associated with a TRK kinase or NTRK gene is selected from hepatocellular carcinoma, breast cancer, bladder cancer, colorectal cancer, melanoma, mesothelioma, lung cancer, prostate cancer, membranous cancer, pancreatic cancer, esophageal cancer, gastric cancer, lymphatic cancer, leukemia, nasopharyngeal carcinoma, testicular cancer, thyroid cancer, squamous cell carcinoma, glioblastoma, neuroblastoma, uterine cancer, and rhabdomyosarcoma.

[0153] In some preferred embodiments, the disease or condition associated with a TRK kinase or NTRK gene is a solid tumor, such as melanoma, breast tumor, neuroblastoma, glioblastoma, Ewing's sarcoma, retinoblastoma, and the like.

[0154] In some other embodiments, the disease or condition associated with a TRK kinase or NTRK gene is a cell proliferative disorder, such as, but not limited to, benign prostatic hyperplasia, familial adenomatous disease, polyposis, neurofibromatosis, psoriasis, atherosclerosis and diseases associated with vascular smooth muscle cell proliferation and neointima formation, such as restenosis after angioplasty or surgery, pulmonary fibrosis, arthritis, glomerulonephritis, retinal pathologies (including diabetic retinopathy, retinopathy of prematurity, and age-related macular degeneration), vascular transplant disease (e.g., as may occur after vascular or organ transplantation), acromegaly and acromegaly sequelae.

[0155] In some other embodiments, the disease or condition associated with a TRK kinase or NTRK gene is inflammation, including, but not limited to, (1) degenerative inflammation, (2) bleeding inflammation (serous inflammation, fibrinous inflammation, suppurative inflammation, hemorrhagic inflammation, necrotizing inflammation, catarrhal inflammation), (3) proliferative inflammation, and (4) specific inflammation (tuberculosis, syphilis, leprosy, lymphogranulomatosis, etc.).

[0156] In some other embodiments, the disease or condition associated with a TRK kinase or NTRK gene is pain, including, but not limited to, inflammatory pain, arthritic pain, complex regional pain syndrome, lower back pain, musculoskeletal pain, neuropathic pain, chronic pain, cancer-related pain, acute pain, post-operative pain, etc.

[0157] In some other embodiments, the disease or condition associated with a TRK kinase or NTRK gene is a neurodegenerative disease, including, but not limited to, Alzheimer's disease and Parkinson's disease.

[0158] The "therapeutically effective amount" of the compound of the present application for treating the above-mentioned diseases can be appropriately determined by experienced physicians or researchers based on factors such as the patient's condition, physical condition, severity of the disease, and route of administration.

[0159] The present invention will now be further described with reference to specific examples. [Example]

[0160] The examples described herein below are used to illustrate various aspects and embodiments of the present invention, and are not intended to limit the scope of the present invention in any way.

[0161] Unless otherwise specified, all raw materials and reagents are commercially available. The instruments used in the synthesis experiments and product analysis and detection are all common instruments and devices commonly used in organic synthesis. All reaction and test conditions not specifically specified are common reaction and test conditions commonly used by those skilled in the art and can be obtained by referring to relevant technical literature or instruction manuals.

[0162] Example 1: N-(1-(1-(3,5-difluorophenyl)ethyl)-3-(1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazol-5-amine [ka]

[0163] Synthesis of Compound 1: [ka]

[0164] Synthetic Route to Intermediate 1-8: [ka]

[0165] Synthesis method: Intermediate 1-1 Synthesis of 5-nitro-1H-indazole-3-formaldehyde Sodium nitrite (1.7 g, 24.70 mmol) was dissolved in 9 mL of DMF and 5 mL of water, cooled to 0 °C, and 3 N HCl (7.2 mL, 21.61 mmol) was slowly added dropwise and allowed to react for 10 minutes. At 0 °C, a solution of 5-nitro-1H-indole (501 mg, 3.10 mmol) in DMF (6 mL) was added to the reaction mixture. After the addition was complete, the mixture was heated to 80 °C and allowed to react overnight. Extracted three times with ethyl acetate, the combined organic phases were washed three times with water, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 370 mg of intermediate 1-1, with a yield of 62.6%.

[0166] 1 H NMR(400MHz,DMSO)δ14.71(brs,1H),10.26(s,1H),8.95(d,J=8.0Hz,1H),8.33(dd,J=8.0Hz,J=12.0Hz,1H),7.93(d,J=16.0Hz,1H).

[0167] Intermediate 1-2 Synthesis of 5-nitro-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole-3-formaldehyde Intermediate 1-1 (370 mg, 1.94 mmol) was dissolved in 30 mL of DCM, p-toluenesulfonic acid (333 mg, 1.94 mmol) was added, and the mixture was stirred for 2 minutes. 3,4-Dihydro-2H-pyran (326 mg, 3.87 mmol) was added to the reaction mixture, and the mixture was allowed to react at room temperature for 2 hours. Water was added to the reaction mixture, and the mixture was extracted twice with DCM. The combined organic phases were washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel column to obtain 380 mg of Intermediate 1-2. The yield was 71.3%.

[0168] 1 H NMR(400MHz,CDCl3)δ10.30(s,1H),9.26(d,J=4.0Hz,1H),8.39-8.36(m,1H),7.82(d,J=8.0Hz,1H),5.93-5 .90(m,1H),4.04-3.99(m,1H),3.86-3.80(m,1H),2.60-2.52(m,1H),2.23-2.20(m,2H),1.84-1.78(m,3H).

[0169] Intermediate 1-9 Synthesis of tert-butyl 2,5-dihydro-1H-pyrrole-1-carboxylate 3-Pyrroline (10.0 g, 0.15 mol) was dissolved in 400 mL of dichloromethane and triethylamine (40.6 mL, 0.29 mol), cooled to 0°C, (Boc)2O (37.9 g, 0.17 mol) was slowly added, and the mixture was allowed to react at room temperature overnight. Water was added, and the mixture was extracted twice with dichloromethane. The combined organic phases were washed three times with water, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel column to obtain intermediate 1-9. The yield was 91.0%.

[0170] Intermediate 1-10 Synthesis of tert-butyl 6-oxa-3-azabicyclo[3.1.0]hexane-3-carboxylate Intermediate 1-9 (24.5 g, 0.15 mol) was dissolved in 450 mL of dichloromethane and cooled to 0°C. m-Chloroperbenzoic acid (37.5 g, 0.22 mol) was added in portions and allowed to react overnight at room temperature. Saturated sodium thiosulfate (40 mL) was added and stirred for 30 minutes. The aqueous phase was extracted twice with dichloromethane, washed with saturated potassium carbonate solution, water and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel column to obtain intermediate 1-10. The yield was 84.9%.

[0171] 1 H NMR(400MHz,CDCl3)δ3.85(d,J=12.0Hz,1H),3.77(d,J=12.0Hz,1H),3.69-3.67(m,2H),3.36-3.30(m,2H),1.45(s,9H).

[0172] Intermediate 1-11 Synthesis of tert-butyl 3-azido-4-hydroxypyrrolidino-1-carboxylate Intermediate 1-10 (20.8 g, 0.12 mol) was dissolved in 150 mL of 1,4-dioxane and 50 mL of water, sodium azide (24.0 g, 0.37 mol) was added, and the mixture was heated to 106 °C and reacted for 18 hours. The mixture was cooled to room temperature, 100 mL of saturated brine was added, and the mixture was extracted with dichloromethane (250 mL × 4). The organic phases were combined, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated to obtain Intermediate 1-11. The yield was 100%.

[0173] 1 H NMR (400MHz, CDCl3) δ4.27-4.24(m,1H),3.94(s,1H),3.73-3.59(m,2H),3.41-3.36(m,2H),1.47(s,9H).

[0174] Intermediate 1-12 Synthesis of tert-butyl 3-azido-4-((methanesulfonyl)oxy)pyrrolidine-1-carboxylate Intermediate 1-11 (28.0 g, 0.12 mol) was dissolved in 350 mL of dichloromethane and triethylamine (37.3 g, 0.37 mol), cooled to 0°C, and methanesulfonyl chloride (16.9 g, 0.15 mol) was slowly added dropwise. After the addition was completed, the mixture was reacted at room temperature for 2 hours. Water was added to quench the reaction, and the mixture was extracted twice with dichloromethane. The organic phases were combined, washed with saturated sodium bicarbonate solution, water, and saturated brine, dried over anhydrous sodium sulfate, and concentrated to obtain Intermediate 1-12. The yield was 98.0%.

[0175] Intermediate 1-13 Synthesis of tert-butyl 3,4-diazidopyrrolidine-1-carboxylate Intermediate 1-12 (36.9 g, 0.12 mol) was dissolved in 250 mL of DMF, sodium azide (23.5 g, 0.36 mol) was added, the mixture was heated to 90 ° C, reacted for 2 days, cooled to room temperature, added 750 mL of water, extracted with methyl tert-butyl ether (400 mL * 4), the organic phases were combined, washed with saturated brine, dried over anhydrous sodium sulfate, and purified on a silica gel column to obtain intermediate 1-13, the yield was 62.2%.

[0176] Intermediate 1-8 Synthesis of tert-butyl 3,4-diaminopyrrolidinone-1-carboxylate Intermediate 1-13 (18.9 g, 0.08 mol) was dissolved in 200 mL of methanol, 10% Pd / C was added, the mixture was purged with hydrogen gas three times, heated to 40 °C, reacted for 2 days, filtered, and concentrated to obtain intermediate 1-8 in a 78% yield.

[0177] 1 H NMR (400MHz, CDCl3) δ3.51-3.49(m,2H),3.40-3.36(m,2H),3.21-3.11(m,2H),1.47(s,9H).

[0178] Intermediate 1-3 Synthesis of tert-butyl 2-(5-nitro-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-3a,4,6,6a-tetrahydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 1-2 (300 mg, 1.09 mmol) was dissolved in 20 mL of tert-butyl alcohol, and tert-butyl 3,4-diaminopyrrolidine-1-carboxylate (219 mg, 1.09 mmol), iodine (415 mg, 1.63 mmol), and potassium carbonate (452 mg, 3.27 mmol) were added. The mixture was heated to 70 °C and reacted for 3 hours. After the reaction was completed, the mixture was cooled to room temperature and quenched by adding 5% aqueous sodium thiosulfate solution. The mixture was extracted with EA, the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 410 mg of intermediate 1-3. The yield was 82.4%.

[0179] Intermediate 1-4 Synthesis of tert-butyl 2-(5-nitro-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 1-3 (400 mg, 0.88 mmol) was dissolved in 5 mL of DMSO, and IBX (491 mg, 1.75 mmol) was added. The mixture was heated to 50°C and reacted for 3 hours. After the reaction was completed, the mixture was cooled to room temperature, and water was added to quench the reaction. The mixture was extracted with EA, and the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 270 mg of intermediate 1-4. The yield was 67.8%.

[0180] Intermediate 1-5 Synthesis of tert-butyl 2-(5-nitro-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 1-4 (270 mg, 0.59 mmol) was dissolved in 25 mL of THF, cooled to 0 °C, and NaH (60%) (35.6 mg, 0.89 mmol) was added. The mixture was stirred for 10 min, and then SEMCl (149 mg, 0.89 mmol) was added. After the addition was complete, the mixture was warmed to room temperature and stirred. After the reaction was complete, the mixture was quenched by adding water, extracted with EA, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel column to obtain 209 mg of intermediate 1-5. The yield was 60.2%.

[0181] 1 H NMR(400MHz,CDCl3)δ9.51(d,J=8.0Hz,1H),8.33(d,J=8.0Hz,1H),7.69(d,J=8.0 Hz,1H),5.99-5.94(m,1H),5.90-5.87(m,1H),5.81-5.79(m,1H),4.63-4.52(m,4H) ),4.00-3.98(m,1H),3.79-3.77(m,1H),3.62-3.56(m,2H),2.55-2.52(m,1H),2. 20-2.17(m,2H),1.79-1.76(m,3H),1.54(s,9H),0.95-0.89(m,2H),-0.08(s,9H).

[0182] Intermediate 1-6 Synthesis of tert-butyl 2-(5-amino-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 1-5 (200 mg, 0.34 mmol) was dissolved in 60 mL of methanol, 10% Pd / C (20 mg) was added, the atmosphere was purged with hydrogen gas three times, and the mixture was reacted at room temperature for 3 hours. After the reaction was completed, the mixture was filtered and concentrated to obtain 173 mg of intermediate 1-6, with a yield of 91.2%.

[0183] Intermediate 1-7 Synthesis of tert-butyl 2-(5-((1-(3,5-difluorophenyl)ethyl)amino)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 1-6 (20.0 mg, 0.04 mmol) and 3,5-difluoroacetophenone (6.8 mg, 0.04 mmol) were dissolved in 5 mL of toluene, and one drop of glacial acetic acid was added. The mixture was heated to 80°C and reacted overnight. After cooling to room temperature, sodium triacetylborohydride (9.9 mg, 0.05 mmol) was added to the reaction mixture, and the mixture was reacted at room temperature for 3 hours. The mixture was quenched by adding water, extracted with EA, washed with saturated brine, concentrated, and purified to obtain 9 mg of intermediate 1-7. The yield was 35.9%.

[0184] Synthesis of Compound 1: N-(1-(1-(3,5-difluorophenyl)ethyl)-3-(1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazol-5-amine Intermediate 1-7 (10.0 mg, 0.05 mmol) was dissolved in 2 mL of methanol, 1 mL of concentrated hydrochloric acid was added, and the mixture was reacted at 50°C for 5 hours, concentrated, dissolved in 5 mL of methanol, 0.5 mL of aqueous ammonia was added, concentrated, and purified by preparative plate to obtain 2.1 mg of the final product, with a yield of 38.3%.

[0185] 1 H NMR(400MHz,CD3OD)δ7.33(d,J=8.0Hz,1H),7.18(d,J=4.0Hz,1H),7.10-7.06(m,2H),6.98(dd,J =4.0Hz,J=8.0Hz,1H),6.74-6.69(m,1H),4.70-4.65(m,1H),4.18(s,4H),1.55(d,J=8.0Hz,3H).

[0186] Example 2: N-(3,5-Difluorobenzyl)-3-(5-(piperidin-4-ylmethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazol-5-amine [ka]

[0187] Synthesis of Compound 2: [ka]

[0188] Synthesis method: Intermediate 2-1 Synthesis of tert-butyl 2-(5-((3,5-difluorobenzyl)amino)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 1-6 (80.0 mg, 0.14 mmol) and 3,5-difluorobenzaldehyde (24.6 mg, 0.17 mmol) were dissolved in 5 mL of toluene, and one drop of glacial acetic acid was added. The mixture was heated to 80°C and reacted overnight. After cooling to room temperature, sodium triacetylborohydride (45.9 mg, 0.22 mmol) was added to the reaction mixture and reacted at room temperature for 3 hours. The mixture was quenched by adding water, extracted with EA, washed with saturated brine, concentrated, and purified to obtain 79 mg of intermediate 2-1. The yield was 80.5%.

[0189] Intermediate 2-2 Synthesis of N-(3,5-difluorobenzyl)-1-(tetrahydro-2H-pyran-2-yl)-3-(1-((2-(trimethylsilyl)ethoxy)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazol-5-amine Intermediate 2-1 (79.0 mg, 0.12 mmol) was dissolved in 20 mL of DCM, ZnBr (105.0 mg, 0.46 mmol) was added, and the mixture was stirred at room temperature. After the reaction was completed, the mixture was quenched by adding water, extracted with DCM, and the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated to obtain 60.2 mg of intermediate 2-2, with a yield of 89.4%.

[0190] Intermediate 2-3 Synthesis of tert-butyl 4-((2-(5-((3,5-difluorobenzyl)amino)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)methyl)piperidine-1-carboxylate Intermediate 2-2 (67.5 mg, 0.12 mmol) and tert-butyl 4-formylpiperidine-1-carboxylate (29.7 mg, 0.14 mmol) were dissolved in 10 mL of DCM and stirred at room temperature for 10 minutes. Sodium triacetylborohydride (37.0 mg, 0.17 mmol) was added and the reaction was allowed to proceed at room temperature for 3 hours. The reaction was quenched by adding water, extracted with DCM, washed with saturated brine, concentrated, and purified to obtain 24.9 mg of intermediate 2-3, with a yield of 27.5%.

[0191] Compound 2: Synthesis of N-(3,5-difluorobenzyl)-3-(5-(piperidin-4-ylmethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazol-5-amine Intermediate 2-3 (24.9 mg, 0.03 mmol) was dissolved in 2 mL of methanol, 1 mL of concentrated hydrochloric acid was added, and the mixture was reacted at 50°C for 5 hours, concentrated, dissolved in 5 mL of methanol, 0.5 mL of aqueous ammonia was added, concentrated, and purified by preparative plate to obtain 5.1 mg of the final product, with a yield of 34.4%.

[0192] 1H NMR(400MHz,CD3OD)δ7.37(d,J=8.0Hz,1H),7.30(d,J=4.0Hz,1H),7.08(dd,J=4.0Hz,J=16.0Hz,2H),7.01(dd,J=4.0Hz,J=8.0Hz,1H),6.80-6.75( m,1H),4.46(s,2H),3.90(s,4H),3.19-3.15(m,2H),2.78-2.72(m,4H),2. 06-2.04(m,1H),1.96-1.91(m,2H),1.81-1.78(m,1H),1.62-1.57(m,1H).

[0193] Example 3: 5-(3,5-difluorobenzyl)-3-(5-(methylsulfonyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole [ka]

[0194] Synthesis of compound 3: [ka]

[0195] Synthesis method: Intermediate 3-1 Synthesis of 5-bromo-1H-indazole-3-formaldehyde Sodium nitrite (1.41 g, 20.4 mmol) was dissolved in 10 mL of water, 10 mL of DMF was added, and 3 M HCl (2.29 mL, 6.89 mmol) was added dropwise at 0 °C. The mixture was stirred for 10 minutes. 5-Bromoindole (500 mg, 2.55 mmol) in 10 mL of DMF was added to the reaction mixture, and the mixture was allowed to react at room temperature for 3 hours. Water was added to the reaction mixture, and the mixture was extracted twice with ethyl acetate. The combined organic phases were washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel column to obtain 410 mg of intermediate 3-1 (71.4% yield).

[0196] 1H NMR(400MHz,CDCl3)δ10.28(s,1H),8.53(d,J=1.1Hz,1H),7.61(dd,J=8.9,1.8Hz,1H),7.49(d,J=8.8,1H).

[0197] Intermediate 3-2 Synthesis of 5-bromo-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole-3-formaldehyde Intermediate 3-1 (300 mg, 1.33 mmol) was dissolved in 20 mL of DCM, p-toluenesulfonic acid (279 mg, 1.46 mmol) was added, and the mixture was stirred for 2 minutes. A solution of 3,4-dihydro-2H-pyran (168 mg, 2.0 mmol) in DCM (3 mL) was added to the reaction mixture, and the mixture was allowed to react at room temperature for 1 hour. Water was added to the reaction mixture, and the mixture was extracted twice with DCM. The combined organic phases were washed with saturated sodium bicarbonate solution and saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel column to obtain 300 mg of intermediate 3-2 in a 72.8% yield.

[0198] Intermediate 3-3 Synthesis of 5-bromo-3-(dimethoxymethyl)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole Intermediate 3-2 (50.0 mg, 0.16 mmol), trimethyl orthoformate (20.6 mg, 0.19 mmol), and p-toluenesulfonic acid (2.8 mg, 0.02 mmol) were dissolved in 5 mL of methanol and reacted at room temperature for 2 hours. The mixture was quenched by adding water, extracted with EA, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified to obtain 23 mg of intermediate 3-3. The yield was 40.0%.

[0199] 1 H NMR(400MHz,CDCl3)δ8.13(d,J=4.0Hz,1H),7.52-7.46(m,2H),5.74(s,1H),5.72-5.69(m,1H),4.08- 4.04(m,1H),3.78-3.72(m,1H),3.47(s,6H),2.52-2.48(m,1H),2.15-2.04(m,2H),1.80-1.71(m,3H).

[0200] Intermediate 3-4 Synthesis of 3-(dimethoxymethyl)-1-(tetrahydro-2H-pyran-2-yl)-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-indazole Intermediate 3-3 (20.0 mg, 0.06 mmol), pinacol boronic acid ester (28.6 mg, 0.11 mmol), Pd(dppf)Cl (4.1 mg, 0.006 mmol), and potassium acetate (16.6 mg, 0.17 mmol) were dissolved in 10 mL of 1,4-dioxane, purged with nitrogen gas three times, heated to 100 °C, and reacted overnight. The mixture was quenched by adding water, extracted with EA, and the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by thin-layer chromatography to obtain 15 mg of intermediate 3-4 (yield: 66.2%).

[0201] 1 H NMR(400MHz,CDCl3)δ8.45(s,1H),7.82(dd,J=4.0Hz,J=8.0Hz,1H),7.58(dd,J=8.0,4.0Hz,1H),5.81(s,1H),5.76(dd,J=8.0,4.0 Hz,1H),4.11-4.07(m,1H),3.78-3.75(m,1H),3.48(s,6H),2.60-2.51(m,1H),2.16-2.03(m,2H),1.79-1.60(m,3H),1.38(s,12H).

[0202] Intermediate 3-5 Synthesis of 5-(3,5-difluorobenzyl)-3-(dimethoxymethyl)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole Intermediate 3-4 (175 mg, 0.44 mmol), 3,5-difluorobromobenzyl (180 mg, 0.87 mmol), Pd(PPh3)4 (50.2 mg, 0.04 mmol), and sodium carbonate (138 mg, 1.31 mmol) were dissolved in 10 mL of tetrahydrofuran and 2 mL of water. The mixture was purged with nitrogen gas three times, heated to 65 °C, and reacted overnight. The mixture was quenched by adding water, extracted with EA, and the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by column chromatography to obtain 148 mg of intermediate 3-5. The yield was 84.5%.

[0203] Intermediate 3-6 Synthesis of 5-(3,5-difluorobenzyl)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole-3-formaldehyde Intermediate 3-5 (50 mg, 0.12 mmol) and p-toluenesulfonic acid (47.3 mg, 0.25 mmol) were dissolved in 10 mL of acetonitrile and reacted at room temperature. After the reaction was completed, the mixture was quenched by adding water, extracted with EA, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and subjected to column chromatography to obtain 27 mg of intermediate 3-6 in a yield of 61.0%.

[0204] 1 H NMR(400MHz,CDCl3)δ10.27(s,1H),8.18(s,1H),7.64(d,J=8.4Hz,1H),7.31-7.28(m,1H),6.74-6.63(m,3H),5.84(dd,J=8 .9,2.9Hz,1H),4.11(s,2H),4.06-3.99(m,1H),3.83-3.77(m,1H),2.63-2.55(m,1H),2.26-2.14(m,2H),1.85-1.74(m,3H).

[0205] Intermediate 3-7 Synthesis of tert-butyl 2-(5-(3,5-difluorobenzyl)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-3a,4,6,6a-tetrahydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 3-6 (50 mg, 0.14 mmol) was dissolved in 10 mL of tert-butyl alcohol, and tert-butyl 3,4-diaminopyrrolidine-1-carboxylate (28.2 mg, 0.14 mmol), iodine (53.4 mg, 0.21 mmol), and potassium carbonate (58.2 mg, 0.42 mmol) were added. The mixture was heated to 70 °C and reacted for 3 hours. After the reaction was completed, the mixture was cooled to room temperature and quenched by adding 5% aqueous sodium thiosulfate solution. The mixture was extracted with EA, the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel column to obtain 68 mg of intermediate 3-7. The yield was 90.2%.

[0206] Intermediate 3-8 Synthesis of tert-butyl 2-(5-(3,5-difluorobenzyl)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 3-7 (65 mg, 0.12 mmol) was dissolved in 10 mL of DMSO, and IBX (67.7 mg, 0.24 mmol) was added. The mixture was heated to 50 °C and reacted for 3 hours. After the reaction was completed, the mixture was cooled to room temperature, and water was added to quench the reaction. The mixture was extracted with EA, and the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 34 mg of intermediate 3-8. The yield was 52.5%.

[0207] 1 H NMR(400MHz,CDCl3)δ8.33(s,1H),7.53(d,J=8.8Hz,1H),7.24(dd,J=8.8,1.7Hz,1H),6.74-6.71(m,1H),6.67-6.60(m,2H),5.72(dd,J=9.6,2.6Hz ,1H),4.60-4.54(m,4H),4.13(s,2H),4.10-4.06(m,1H),3.81-3.75(m,1 H),2.62-2.54(m,1H),2.16-2.06(m,2H),1.85-1.70(m,3H),1.55(s,9H).

[0208] Intermediate 3-9 Synthesis of tert-butyl 2-(5-(3,5-difluorobenzyl)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 3-8 (19.8 mg, 0.04 mmol) was dissolved in 5 mL of THF, cooled to 0 °C, and NaH (60%) (1.9 mg, 0.05 mmol) was added. The mixture was stirred for 15 min, and then SEMCl (6.7 mg, 0.04 mmol) was added. After the addition was complete, the mixture was warmed to room temperature and stirred. After the reaction was complete, water was added to quench the reaction, and the mixture was extracted with EA. The organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 18.1 mg of intermediate 3-9. The yield was 73.4%.

[0209] Intermediate 3-10 Synthesis of 5-(3,5-difluorobenzyl)-1-(tetrahydro-2H-pyran-2-yl)-3-(1-((2-(trimethylsilyl)ethoxy)methyl)-1,4,5,6-tetrahydropyrrole[3,4-d]imidazol-2-yl)-1H-indazole Intermediate 3-9 (182 mg, 0.27 mmol) was dissolved in 20 mL of DCM, ZnBr (24 mg, 1.10 mmol) was added, and the mixture was stirred at room temperature. After the reaction was completed, the mixture was quenched with water, extracted with DCM, and the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated to give 140 mg of intermediate 3-10, with a yield of 90.3%.

[0210] Compound 3: Synthesis of 5-(3,5-difluorobenzyl)-3-(5-(methylsulfonyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole Intermediate 3-10 (30.0 mg, 0.05 mmol) was added to 5 mL of DCM, and TEA (8.0 mg, 0.08 mmol) and methanesulfonyl chloride (7.3 mg, 0.06 mmol) were added to the solution. After the reaction was completed, the mixture was quenched with water and extracted with DCM. The organic phases were combined, washed with saturated brine, and concentrated. The concentrate was dissolved in 4 mL of methanol, 2 mL of concentrated hydrochloric acid was added, and the mixture was reacted at 50 °C for 3 hours. The mixture was then concentrated, dissolved in 5 mL of methanol, 0.5 mL of aqueous ammonia was added, concentrated, and purified using a preparative plate to obtain 5.6 mg of the final product, a yield of 24.6%.

[0211] 1 H NMR(400MHz,CD3OD)δ8.18(s,1H),7.53(d,J=8.4Hz,1H),7.32(dd,J=8.7,4.0Hz,1H) ,6.91-6.85(m,2H),6.78-6.73(m,1H),4.66-4.52(m,4H),3.65(s,2H),3.01(s,3H).

[0212] Example 4: 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(1-methylpiperidin-4-yl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole [ka]

[0213] Synthesis of compound 4: [ka]

[0214] Synthesis method: Intermediate 4-1 Synthesis of 1-(3,5-difluorophenyl)ethanol 3,5-Difluoroacetophenone (2.1 g, 13.50 mmol) was dissolved in 20 mL of methanol, and sodium cyanoborohydride (1.3 g, 20.21 mmol) was added in portions at 0°C. After the addition, the mixture was stirred at room temperature for 1 hour to complete the reaction. Water was added to the reaction mixture, and the mixture was extracted twice with ethyl acetate. The combined organic phases were washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated to obtain 2.13 g of crude intermediate 4-1, with a yield of 100.1%.

[0215] Intermediate 4-2 Synthesis of ethyl 1-(3,5-difluorophenyl)methanesulfonate Intermediate 4-1 (2.13 g, 13.50 mmol) was dissolved in 20 mL of dichloromethane, triethylamine (4.1 g, 40.41 mmol) was added, and methanesulfonyl chloride (1.9 g, 16.20 mmol) was added dropwise at 0°C. After the addition was complete, the mixture was stirred at room temperature for 1 hour to complete the reaction. Water was added to the reaction mixture, and the mixture was extracted twice with ethyl acetate. The organic phases were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 3.2 g of crude intermediate 4-2. The yield was 100%.

[0216] Intermediate 4-3 Synthesis of 5-(1-(3,5-difluorophenyl)ethoxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole-3-formaldehyde Intermediate 4-2 (2.2 g, 9.30 mmol) and 5-hydroxy-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole-3-formaldehyde (2.3 g, 18.61 mmol) were dissolved in 20 mL of DMF, and cesium carbonate (6.1 g, 0.82 mmol) was added. The reaction was carried out at 60 °C for 2 hours to complete the reaction. Water was added to the reaction solution, and the mixture was extracted twice with ethyl acetate. The organic phases were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 3.1 g of intermediate 4-3. The yield was 86.2%.

[0217] 1H NMR(400MHz,CDCl3)δ10.19(s,1H),7.72-7.45(m,2H),7.19-7.16(m,1H),7.00-6.92(m,2H),6.73-6.67(m,1H),5.81-5.77(m,1H),5.43 (q,J=6.3Hz,1H),4.03-3.98(m,1H),3.81-3.75(m,1H),2.56-2.49(m,1H),2.26-2.03(m,2H),1.82-1.71(m,3H),1.67(d,J=6.3Hz,3H).

[0218] Intermediate 4-4 Synthesis of tert-butyl 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-3a,4,6,6a-tetrahydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 4-3 (3.1 g, 8.01 mmol) was dissolved in 30 mL of tert-butyl alcohol, and tert-butyl 3,4-diaminopyrrolidine-1-carboxylate (1.6 g, 8.01 mmol), iodine (3.1 g, 12.11 mmol), and potassium carbonate (3.3 g, 24.02 mmol) were added. The mixture was heated to 70 °C and reacted for 3 hours. After the reaction was completed, the mixture was cooled to room temperature and quenched by adding 5% aqueous sodium thiosulfate solution. The mixture was extracted with EA, the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 3.6 g of intermediate 4-4 in a 79.1% yield.

[0219] 1H NMR(400MHz,CDCl3)δ7.74(s,1H),7.49(dd,J=9.1Hz,3.2Hz,1H),7.11(dd,J=9.1Hz,2.4Hz ,1H),7.00(d,J=7.3Hz,2H),6.69(t,J=9.0Hz,1H),5.70-5.67(m,1H),5.49-5.44(m,1H),5. 00-4.80(m,1H),4.52-4.31(m,1H),4.03-3.99(m,1H),3.77-3.66(m,5H),2.56-2.47(m,1H ),2.18-2.10(m,1H),2.06-2.02(m,1H),1.79-1.72(m,3H),1.66-1.64(m,3H),1.45(s,9H).

[0220] Intermediate 4-5 Synthesis of tert-butyl 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-4,6-dihydropyrrole[3,4-d]imidazole-5(1H)-carboxylate Intermediate 4-4 (1.3 g, 2.31 mmol) was dissolved in 10 mL of DMSO, and IBX (1.3 g, 4.60 mmol) was added. The mixture was heated to 50 °C and reacted for 3 hours. After the reaction was completed, the mixture was cooled to room temperature, and water was added to quench the reaction. The mixture was extracted with EA, and the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 560 mg of intermediate 4-5. The yield was 43.2%.

[0221] Intermediate 4-6 Synthesis of tert-butyl 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 4-5 (790 mg, 1.40 mmol) was dissolved in 10 mL of THF, cooled to 0 °C, and NaH (60%) (96.4 mg, 4.20 mmol) was added. The mixture was stirred for 15 min, and then SEMCl (280 mg, 1.68 mmol) was added. After the addition was complete, the mixture was warmed to room temperature and stirred. After the reaction was complete, water was added to quench the reaction, and the mixture was extracted with EA. The organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 810 mg of intermediate 4-6. The yield was 83.3%.

[0222] Intermediate 4-7 Synthesis of 5-(1-(3,5-difluorophenyl)ethoxy)-1-(tetrahydro-2H-pyran-2-yl)-3-(1-((2-(trimethylsilyl)ethoxy)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole Intermediate 4-6 (810.0 mg, 1.20 mmol) was dissolved in 20 mL of DCM, ZnBr (1.1 g, 4.70 mmol) was added, and the mixture was stirred at room temperature. After the reaction was completed, the mixture was quenched with water, extracted with DCM, and the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated to obtain 660 mg of intermediate 4-7. The yield was 95.2%.

[0223] Synthesis of Compound 4: 5-(1-(3,5-difluorophenyl)ethoxy)3-(5-(1-methylpiperidin-4-yl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole Intermediate 4-7 (15.0 mg, 0.03 mmol) and 1-methylpiperidin-4-one (4.3 mg, 0.04 mmol) were dissolved in 5 mL of DCM and stirred at room temperature for 10 minutes. Sodium triacetoxyborohydride (8.01 mg, 0.04 mmol) was added and stirred at room temperature for 3 hours. After completion of the reaction, the mixture was quenched with water, extracted with DCM, washed with saturated brine, and concentrated. The purified product was purified on a silica gel plate. The purified product was dissolved in 2 mL of methanol, added with 1 mL of concentrated hydrochloric acid, and reacted at 50 °C for 5 hours. The product was then concentrated, dissolved in 5 mL of methanol, added with 0.5 mL of aqueous ammonia, concentrated, and purified on a preparative plate to give 1.9 mg of the final product. The combined yield of the two steps was 15.8%.

[0224] 1 H NMR(400MHz,CD3OD)δ7.67(d,J=4.0Hz,1H),7.50(d,J=8.0Hz,1H),7.19(dd,J=4.0Hz,J=8.0Hz,1H),7.10(dd,J=4.0Hz,J=8.0Hz,2H),6.84-6.79(m, 1H),5.61-5.56(m,1H),4.36(s,4H),3.71-3.59(m,2H),3.19-3.15(m,3H) ,2.93(s,3H),2.48-2.47(m,2H),2.06-2.02(m,2H),1.67(d,J=8.0Hz,3H).

[0225] Example 5: 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-N,N-dimethyl-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)formamide [ka]

[0226] Synthesis of compound 5: [ka]

[0227] Synthesis method: Synthesis of Compound 5: 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-N,N-dimethyl-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)formamide Intermediate 4-7 (30.0 mg, 0.05 mmol) was dissolved in 10 mL of dichloromethane, triphosgene (15.3 mg, 0.05 mmol) was added, the mixture was cooled to 0 °C, triethylamine (10.4 mg, 0.10 mmol) was added dropwise, and the mixture was allowed to react for 0.5 h. Dimethylamine hydrochloride (5.05 mg, 0.06 mmol) was added, and the mixture was allowed to warm to room temperature. After the reaction was complete, the mixture was quenched with water, extracted with DCM, and the organic phases were combined, washed with saturated brine, and concentrated. The concentrate was dissolved in 4 mL of methanol, 2 mL of concentrated hydrochloric acid was added, and the mixture was allowed to react at 50 °C for 5 h. The mixture was then concentrated, dissolved in 5 mL of methanol, 0.5 mL of aqueous ammonia was added, concentrated, and purified using a preparative plate to obtain 4.8 mg of the final product in a yield of 20.4%.

[0228] 1 H NMR(400MHz,CD3OD)δ7.69(d,J=2.3Hz,1H),7.46(d,J=9.0Hz,1H),7.17(dd,J=9.0,2.4Hz,1H),7.13-7.0 8(m,2H),6.84-6.78(m,1H),5.58(q,J=6.4Hz,1H),4.71-4.63(m,4H),2.99(s,6H),1.66(d,J=6.4Hz,3H).

[0229] Example 6: 2-(Dimethylamino)ethyl 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate [ka]

[0230] Synthesis of compound 6: [ka]

[0231] Synthesis method: Synthesis of Compound 6: 2-(dimethylamino)ethyl 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 4-7 (50.0 mg, 0.08 mmol) was dissolved in THF, cooled to 0°C, triphosgene (25.1 mg, 0.08 mmol) was added, and the mixture was allowed to react for 5 minutes. Triethylamine (84.2 mg, 0.80 mmol) was slowly added, and the mixture was allowed to react at room temperature for 0.5 hours. 2-(dimethylamino)ethan-1-ol (38.1 mg, 0.42 mmol) and DMAP (5.0 mg, 0.04 mmol) were added to the reaction mixture, and the mixture was heated to 60°C and allowed to react for 1.5 hours. The reaction mixture was added with water and extracted twice with EA. The combined organic phases were washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel plate. The purified concentrate was dissolved in 2 mL of methanol, added with 1 mL of concentrated hydrochloric acid, reacted at 50°C for 6 hours, concentrated, dissolved in 3 mL of methanol, added with 0.5 mL of aqueous ammonia, concentrated, and purified on a silica gel plate to obtain 15 mg of the final product in a yield of 35.0%.

[0232] 1 H NMR(400MHz,CD3OD)δ7.68(d,J=4.0Hz,1H),7.46(d,J=8.0Hz,1H),7.20-7.14(m,1H),7.14-7.08(m,2H),6.85-6.78(m,1H) ),5.58(q,J=8.0Hz,1H),4.72-4.50(m,4H),4.35(t,J=8.0Hz,2H),2.82-2.74(m,2H),2.41(s,6H),1.66(d,J=8.0Hz,3H).

[0233] Example 7: (2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)(1-methylpiperidin-4-yl)methanone [ka]

[0234] Synthesis of compound 7: [ka]

[0235] Synthesis method: Synthesis of Compound 7 (2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)(1-methylpiperidin-4-yl)methanone Intermediate 4-7 (30.0 mg, 0.05 mmol), HATU (29.1 mg, 0.10 mmol), N,N-diisopropylethylamine (13.2 mg, 0.10 mmol), and 1-methylpiperidine-4-carboxylic acid (14.0 mg, 0.10 mmol) were dissolved in 20 mL of DCM and stirred for 2 hours. The reaction was quenched by adding water. After two extractions with DCM, the combined organic phases were washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated. The resulting product was dissolved in 2 mL of methanol and 1 mL of concentrated hydrochloric acid, reacted at 50 °C for 2 hours, concentrated, dissolved in 3 mL of methanol, added with 1 mL of aqueous ammonia, concentrated, and purified on a preparative plate to obtain 8 mg of the final product in a yield of 43.7%.

[0236] 1H NMR(400MHz,CD3OD)δ7.67(d,J=1.8Hz,1H),7.45(d,J=9.0Hz,1H),7.21-7.03(m,3H),6.78(t,J=9.1Hz,1H),5.55(q,J=6.2Hz,1H), 4.80-4.59(m,4H),3.17-3.14(m,2H),2.75-2.65(m,1H),2.48(s,3H),2.46-2.41(m,2H),1.95-1.90(m,4H),1.63(d,J=6.4Hz,3H).

[0237] Example 8: 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-5-(1-methylpiperidin-4-yl)-4,5,6,7-tetrahydro-3H-imidazo[4,5-c]pyridine [ka]

[0238] Synthetic route to compound 8: [ka]

[0239] Synthesis method: Intermediate 8-1 Synthesis of 5-(1-(3,5-difluorophenyl)-4-yl)ethoxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole-3-nitrile 5-(1-(3,5-Difluorophenyl)ethoxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole-3-formaldehyde (200 mg, 0.52 mmol) and triethylamine (157 mg, 1.55 mmol) were dissolved in 10 mL of acetonitrile, and hydroxylamine hydrochloride (53.9 mg, 0.78 mmol) was added to the solution and reacted at 60 °C for 3 hours. The reaction mixture was cooled to room temperature, and triethylamine (419 mg, 4.14 mmol) and acetic anhydride (211 mg, 2.07 mmol) were added to the reaction mixture and reacted at 80 °C for 16 hours. Water was added to the reaction solution, and the mixture was extracted twice with dichloromethane. The organic phases were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel column to obtain 145 mg of intermediate 8-1, with a yield of 73.1%.

[0240] 1 H NMR(400MHz,CDCl3)δ7.64-7.60(m,1H),7.19-7.17(m,1H),6.98-6.97(m,1H),6.93-6.89(m,2H),6.73-6.68(m,1H),5.77-5.73(m,1H),5. 34(q,J=6.4Hz,1H),3.94-3.88(m,1H),3.76-3.71(m,1H),2.49-2.41(m,1H),2.14-2.06(m,2H),1.77-1.68(m,3H),1.65(d,J=6.4Hz,3H).

[0241] Intermediate 8-2 Synthesis of 5-(1-(3,5-difluorophenyl)ethoxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole-3-carbamate Intermediate 8-1 (135 mg, 0.35 mmol) was dissolved in 10 mL of methanol, and sodium methoxide (57.1 mg, 1.06 mmol) was added to the reaction solution. The mixture was allowed to react at room temperature for 16 hours. The reaction solution was concentrated to remove methanol, and water was added to the concentrate. The mixture was extracted twice with ethyl acetate. The combined organic phases were washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated to obtain 120 mg of crude product Intermediate 8-2. The yield was 82.0%.

[0242] Intermediate 8-3 Synthesis of tert-butyl 3-(5-(1-(3,5-difluorophenyl)ethoxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole-3-carboximide)-4,4-diethoxypiperidine-1-carboxylate Intermediate 8-2 (110 mg, 0.26 mmol) and tert-butyl 3-amino-4,4-diethoxypiperidine-1-carboxylate (91.6 mg, 0.32 mmol) were dissolved in 10 mL of ethanol, and acetic acid (31.8 mg, 0.53 mmol) was added to the reaction solution. The mixture was reacted at 50°C for 16 hours. The reaction solution was concentrated to remove ethanol, and the concentrate was treated with an appropriate amount of toluene and concentrated to obtain 208 mg of crude intermediate 8-3.

[0243] Intermediate 8-4 Synthesis of 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,5,6,7-tetrahydro-3H-imidazole[4,5-c]pyridine Intermediate 8-3 (200 mg, 0.22 mmol) was dissolved in 10 mL of ethanol, and 5 mL of concentrated hydrochloric acid was added to the reaction solution. The mixture was reacted at 40°C for 16 hours, concentrated, dissolved in 5 mL of methanol, and 0.5 mL of aqueous ammonia was added. The mixture was concentrated and purified on a silica gel column to obtain 78 mg of intermediate 8-4. The yield was 66.2%.

[0244] Synthesis of Compound 8: 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-5-(1-methylpiperidin-4-yl)-4,5,6,7-tetrahydro-3H-imidazo[4,5-c]pyridine Intermediate 8-4 (15.0 mg, 0.04 mmol) was dissolved in 5 mL of NMP, 0.1 mL of acetic acid was added to the solution, and the mixture was stirred at 55 °C for 10 minutes. The mixture was cooled to room temperature, 1-methylpiperidin-4-one (4.3 mg, 0.04 mmol) was added, and the mixture was reacted at room temperature for 16 hours. Sodium triacetoxyborohydride (16.0 mg, 0.08 mmol) was added, and the mixture was reacted at room temperature for 2 hours. 0.5 mL of aqueous ammonia was added to adjust the pH. The mixture was then added with water and extracted twice with ethyl acetate. The organic phases were combined, washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified by preparative plate to obtain 4.2 mg of the final product. The yield was 22.5%.

[0245] 1 H NMR(400MHz,CD3OD)δ7.68(d,J=2.4Hz,1H),7.45(d,J=9.0Hz,1H),7.16(dd,J=9.0,2 .4Hz,1H),7.12-7.10(m,2H),6.83-6.79(m,1H),5.58(q,J=6.3Hz,1H),3.80(s,2H), 3.17-3.10(m,2H),3.04-3.01(m,2H),2.84-2.80(m,2H),2.73-2.70(m,1H),2.43(s, 3H),2.36-2.19(m,2H),2.10-2.03(m,2H),1.84-1.75(m,2H),1.65(d,J=6.3Hz,3H).

[0246] Example 9: (S)-5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(((1-methylpiperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole [ka]

[0247] Synthetic route to compound 9: [ka]

[0248] Synthesis method: Intermediate 9-1 Synthesis of 5-((tert-butyldimethylsilyl)oxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole-3-formaldehyde 5-Hydroxy-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole-3-formaldehyde (427 mg, 1.73 mmol) and imidazole (177 mg, 2.60 mmol) were dissolved in 20 mL of dichloromethane, and TBSCl (0.33 mL, 1.91 mmol) was added dropwise at 0 °C. After the addition was complete, the mixture was stirred at room temperature for 1 hour to complete the reaction. Water was added to the reaction mixture, and the mixture was extracted twice with dichloromethane. The combined organic phases were washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated to obtain 540 mg of crude intermediate 9-1 (86.4% yield).

[0249] Intermediate 9-2 Synthesis of tert-butyl 2-(5-((tert-butyldimethylsilyl)oxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-3a,4,6,6a-tetrahydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 9-1 (540 mg, 1.49 mmol) was dissolved in 20 mL of tert-butyl alcohol, and tert-butyl 3,4-diaminopyrrolidine-1-carboxylate (362 mg, 1.79 mmol), iodine (570 mg, 2.25 mmol), and potassium carbonate (621 mg, 4.49 mmol) were added. The mixture was heated to 70 °C and reacted for 3 hours. After the reaction was completed, the mixture was cooled to room temperature and quenched by adding 5% aqueous sodium thiosulfate solution. The mixture was extracted with EA, the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 530 mg of intermediate 9-2. The yield was 65.3%.

[0250] Intermediate 9-3 Synthesis of tert-butyl 2-(5-((tert-butyldimethylsilyl)oxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 9-2 (530 mg, 0.99 mmol) was dissolved in 10 mL of DMSO, and IBX (548 mg, 1.96 mmol) was added. The mixture was heated to 50 °C and reacted for 3 hours. After the reaction was completed, the mixture was cooled to room temperature, and water was added to quench the reaction. The mixture was extracted with EA, and the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 362 mg of intermediate 9-3. The yield was 68.4%.

[0251] Intermediate 9-4 Synthesis of tert-butyl 2-(5-((tert-butyldimethylsilyl)oxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate Intermediate 9-3 (362 mg, 0.67 mmol) was dissolved in 10 mL of THF, cooled to 0 °C, and NaH (60% w / w, 32.1 mg, 1.33 mmol) was added. The mixture was stirred for 15 min, and then SEMCl (0.14 mL, 0.80 mmol) was added. After the addition was complete, the mixture was warmed to room temperature and stirred. After the reaction was complete, water was added to quench the reaction. The mixture was extracted with EA, the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel column to obtain 365 mg of intermediate 9-4 in an 81.5% yield.

[0252] Intermediate 9-5 Synthesis of 5-((tert-butyldimethylsilyl)oxy)-1-(tetrahydro-2H-pyran-2-yl)-3-(1-((2-(trimethylsilyl)ethoxy)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole Intermediate 9-4 (180 mg, 0.27 mmol) was dissolved in 10 mL of DCM, and ZnBr (303 mg, 1.34 mmol) was added and stirred at room temperature. After the reaction was completed, the mixture was quenched with water, extracted with DCM, and the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated to obtain 153 mg of intermediate 9-5. The yield was 99.9%.

[0253] Intermediate 9-6 Synthesis of tert-butyl 4-((2-(5-((tert-butyldimethylsilyl)oxy)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)methyl)piperidine-1-carboxylate Intermediate 9-5 (153 mg, 0.27 mmol) and tert-butyl 4-formylpiperidine-1-carboxylate (68.7 mg, 0.32 mmol) were dissolved in 10 mL of dichloromethane and stirred at room temperature for 1 hour. Sodium cyanoborohydride (25.3 mg, 0.40 mmol) was added and stirred for 1 hour. After the reaction was completed, water was added to quench the reaction. The mixture was extracted with dichloromethane, the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel column to obtain 165 mg of intermediate 9-6. The yield was 80.1%.

[0254] Intermediate 9-7 Synthesis of 5-((tert-butyldimethylsilyl)oxy)-3-(5-(piperidin-4-ylmethyl)-1-((2-(trimethylsilyl)ethoxy)methyl)-1,4,5,6-tetrahydropyrrole[3,4-d]imidazol-2-yl)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole Intermediate 9-6 (165 mg, 0.22 mmol) was dissolved in 10 mL of DCM, and ZnBr (248 mg, 1.10 mmol) was added and stirred at room temperature. After the reaction was completed, the mixture was quenched with water, extracted with DCM, washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated to obtain 130 mg of intermediate 9-7 (yield: 90.6%).

[0255] Synthesis of Compound 9-8: 5-((tert-butyldimethylsilyl)oxy)-3-(5-(((1-methylpiperidin-4-yl)methyl)-1-((2-(trimethylsilyl)ethoxy)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole Intermediate 9-7 (130 mg, 0.19 mmol) and 30% aqueous formaldehyde (17.5 mg, 0.58 mmol) were dissolved in 5 mL of DCM and stirred for 1 h. Sodium triacetylborohydride (80.5 mg, 0.38 mmol) was added to the solution. After the reaction was completed, the mixture was quenched with water and extracted with DCM. The combined organic phases were washed with saturated brine, concentrated, and purified on a silica gel column to obtain 75.5 mg of intermediate 9-8. The yield was 56.9%.

[0256] Synthesis of Compound 9-9: 3-(5-((1-methylpiperidin-4-yl)methyl)-1-((2-(trimethylsilyl)ethoxy)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-5-ol Intermediate 9-8 (40.0 mg, 0.06 mmol) was dissolved in 5 mL of tetrahydrofuran, cooled to 0°C, and a 1 M solution of TBAF in tetrahydrofuran (0.12 mL, 0.12 mmol) was added to the solution. The mixture was stirred for 30 minutes, quenched by adding water, extracted with ethyl acetate, and the combined organic phases were washed with saturated brine and concentrated to obtain 30.1 mg of crude product 9-9. The yield was 90.1%.

[0257] Intermediate 9-10 Synthesis of 5-((S)-1-(3,5-difluorophenyl)ethoxy)-3-(5-(((1-methylpiperidin-4-yl)methyl)-1-((2-(trimethylsilyl)ethoxy)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazole Intermediate 9-9 (30.1 mg, 0.05 mmol) and (R)-1-(3,5-difluorophenyl)ethyl methanesulfonate (12.5 mg, 0.05 mmol) were dissolved in 10 mL of DMF, and cesium carbonate (35.8 mg, 0.11 mmol) was added. The reaction was allowed to proceed at 60 °C for 2 hours to complete the reaction. Water was added to the reaction mixture, and the mixture was extracted twice with ethyl acetate. The combined organic phases were washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified using a silica gel column to obtain 19.2 mg of intermediate 9-10. The yield was 51.3%.

[0258] Synthesis of Compound 9: (S)-5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(((1-methylpiperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole Intermediate 9-10 (19.2 mg, 0.02 mmol) was dissolved in 4 mL of methanol, 2 mL of concentrated hydrochloric acid was added, and the mixture was reacted at 50°C for 5 hours, concentrated, dissolved in 5 mL of methanol, 0.5 mL of aqueous ammonia was added, concentrated, and purified by preparative plate to obtain 6.2 mg of the final product, with a yield of 46.3%.

[0259] 1 H NMR(400MHz,CD3OD)δ7.64(s,1H),7.46(d,J=9.0Hz,1H),7.15(dd,J=9.1,2.4Hz,1H),7.10-7.05(m,2H),6.81-6.76(m,1H),5.55(q,J=6 .4Hz,1H),4.38(s,4H),3.56(d,J=12.4Hz,2H),3.27-3.20(m,2H),3.16-3.06(m,2H),2.89(s,3H),2.21-2.18(m,3H),1.76-1.62(m,5H).

[0260] Example 10: 1-Methylpiperidin-4-yl-2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate [ka]

[0261] Synthetic route to compound 10: [ka]

[0262] Synthesis method: Synthesis of Compound 10: 1-methylpiperidin-4-yl-2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate 1-Methylpiperidin-4-ol (11.0 mg, 0.10 mmol) was dissolved in 10 mL of DCM and cooled to 0 °C. Triphosgene (15.0 mg, 0.05 mmol) was added and the mixture was allowed to react for 5 minutes. Triethylamine (41.2 mg, 0.40 mmol) was added dropwise to the reaction mixture and stirred for 0.5 hours. Intermediate 4-7 (30.0 mg, 0.05 mmol) was added to the reaction mixture and the mixture was allowed to react for 16 hours. Water was added to quench the reaction. The mixture was extracted twice with DCM, and the combined organic phases were washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated. The resulting product was dissolved in 2 mL of methanol and 1 mL of concentrated hydrochloric acid, reacted at 50 °C for 2 hours, concentrated, dissolved in 3 mL of methanol, added with 1 mL of aqueous ammonia, concentrated, and purified using a preparative plate to obtain 2.2 mg of the final product (yield: 17%).

[0263] 1 H NMR(400MHz,CD3OD)δ7.69(d,J=2.2Hz,1H),7.47(d,J=9.1Hz,1H),7.18-7.09(m,3H),6.83-6.79(m,1H),5.58(q,J=6.2Hz,1H),4.87-4.83 (m,1H),4.63-4.58(m,4H),2.87-2.79(m,2H),2.62-2.52(m,2H),2.43(s,3H),2.08-2.02(m,2H),1.93-1.84(m,2H),1.66(d,J=6.4Hz,3H).

[0264] Example 11: N-(1-(3,5-difluorophenyl)ethyl)-3-(5-(piperidin-4-ylmethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazol-5-amine [ka]

[0265] Synthesis of compound 11: [ka]

[0266] Synthesis method: Intermediate 11-1 Synthesis of 5-nitro-1-(tetrahydro-2H-pyran-2-yl)-3-(1-((2-(trimethylsilyl)ethoxy)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole Intermediate 1-5 (1.40 g, 2.39 mmol) was dissolved in 10 mL of DCM, zinc bromide (1.08 g, 4.79 mmol) was added, the atmosphere was purged with nitrogen, and the mixture was allowed to react at room temperature for 4 hours. After the reaction was completed, the mixture was quenched by adding water, extracted with DCM, the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel column to obtain 780 mg of intermediate 11-1, with a yield of 67.21%.

[0267] C in LC-MS m / z(ESI)[M+H]+ 23 H 33 Calculated for N6O4Si: 484.2; Measured: 484.2.

[0268] Intermediate 11-2 Synthesis of tert-butyl 4-((2-(5-nitro-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)methyl)piperidine-1-carboxylate Intermediate 11-1 (780 mg, 1.61 mmol) was dissolved in 10 mL of 1,2-dichloroethane, and tert-butyl 4-formylpiperidine-1-carboxylate (686 mg, 3.22 mmol) was added. After stirring at room temperature for 2 h, sodium triacetylborohydride (683 mg, 3.22 mmol) was added. After stirring at room temperature for 1 h, the mixture was quenched by adding water, extracted with DCM, the organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, concentrated, and purified on a silica gel column to obtain 650 mg of intermediate 11-2. The yield was 59.01%.

[0269] C in LC-MS m / z(ESI)[M+H]+ 34 H 52 Calculated for N7O6Si: 682.4; Found: 682.4.

[0270] Intermediate 11-3 Synthesis of tert-butyl 4-((2-(5-amino-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)methyl)piperidine-1-carboxylate Intermediate 11-2 (200 mg, 0.29 mmol) was dissolved in 5 mL of ethyl acetate, palladium carbon (10% w / w, 100 mg) was added, and the atmosphere was replaced with hydrogen gas. The mixture was reacted at room temperature under a hydrogen gas atmosphere for 18 hours. After 18 hours, the mixture was suction filtered, concentrated, and purified on a silica gel column to obtain 110 mg of intermediate 11-3, with a yield of 57.49%.

[0271] C in LC-MS m / z(ESI)[M+H]+ 34 H 54Calculated for N7O4Si: 652.4; Found: 652.4.

[0272] Intermediate 11-4 Synthesis of tert-butyl 4-((2-(5-((1-(3,5-difluorophenyl)ethyl)amino)-1-(tetrahydro-2H-pyran-2-yl)-1H-indazol-3-yl)-1-((2-(trimethylsilyl)ethoxy)methyl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)methyl)piperidine-1-carboxylate Intermediate 11-3 (110 mg, 0.17 mmol) was dissolved in 5 mL of toluene, and 1-(3,5-difluorophenyl)ethan-1-one (52.6 mg, 0.34 mmol) was added. The mixture was reacted at 90°C for 18 h. The temperature was lowered to 40°C, and sodium cyanoborohydride (31.8 mg, 0.51 mmol) was added. The mixture was reacted for 4 h. The mixture was quenched by adding water, extracted with EA, and the organic phase was washed with water, dried, concentrated, and purified on a silica gel column to obtain 36 mg of intermediate 11-4. The yield was 27.0%.

[0273] C in LC-MS m / z(ESI)[M+H]+ 42 H 60 Calculated for F2N7O4Si: 792.4; Found: 792.4.

[0274] Synthesis of Compound 11: N-(1-(3,5-difluorophenyl)ethyl)-3-(5-(piperidin-4-ylmethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazol-5-amine Intermediate 11-4 (36 mg, 0.05 mmol) was dissolved in 4 mL of methanol, and 2 mL of concentrated hydrochloric acid was added. The mixture was reacted at 50°C for 3 h, concentrated, dissolved in 5 mL of methanol, and 0.5 mL of aqueous ammonia was added. The mixture was concentrated and purified by preparative plate chromatography to give 7 mg of the final product in a yield of 32.2%.

[0275] C in LC-MS m / z(ESI)[M+H]+ 26 H 30Calculated for F2N7: 478.3; Measured: 478.3.

[0276] Example 12: The compound of Example 12 is prepared using appropriate starting materials according to the synthetic route of Example 11, specifically as follows.

[0277] [Table 1]

[0278] Examples 13 and 14: The compounds of Examples 13 and 14 are prepared using appropriate starting materials according to the synthetic route of Example 3. Specifically, the process is as follows.

[0279] [Table 2]

[0280] Examples 15 to 28: The compounds of Examples 15 to 28 are produced using appropriate starting materials based on the synthetic route of Example 4. Specifically, the process is as follows.

[0281] [Table 3-1] [Table 3-2] [Table 3-3] [Table 3-4] [Table 3-5]

[0282] Example 29: The compound of Example 29 is prepared using appropriate starting materials according to the synthetic route of Example 5, specifically as follows:

[0283] [Table 4]

[0284] Example 30 and Example 31: The compounds of Examples 30 and 31 are prepared using appropriate starting materials according to the synthetic route of Example 6. Specifically, the process is as follows.

[0285] [Table 5]

[0286] Example 32: The compound of Example 32 is prepared using appropriate starting materials according to the synthetic route of Example 9, specifically as follows:

[0287] [Table 6]

[0288] Example 33: The compound of Example 33 is prepared using appropriate starting materials according to the synthetic route of Example 10, specifically as follows:

[0289] [Table 7]

[0290] Examples 34 to 46: The compounds of Examples 34 to 46 are produced using appropriate starting materials based on the synthetic route of Example 4. Specifically, the process is as follows.

[0291] [Table 8-1] [Table 8-2] [Table 8-3] [Table 8-4] [Table 8-5]

[0292] Examples 47 to 48: The compounds of Examples 47 and 48 are produced using appropriate starting materials based on the synthetic route of Example 9. Specifically, the process is as follows.

[0293] [Table 9]

[0294] Activity test experiment: TRKA / B / C kinase inhibitory activity test 1. Materials and Equipment [Table 10]

[0295] 2. Experimental Procedure 2.1 Preparation of 1x kinase reaction buffer 1 volume of 5X kinase reaction buffer and 4 volumes of water; 5 mM MgCl2, 1 mM DTT.

[0296] 2.2 Kinase and substrate preparation: Preparation of 2.5X substrate mix

[0297] [Table 11]

[0298] 2.3 Compound Selection: 1) Dilute the compounds 4-fold with DMSO on a dilution plate, with the starting compound concentration being 1000 nM. 2) Dilute the compound 50-fold with 1X kinase reaction buffer and shake on a shaker for 20 minutes. 3) Prepare 2X kinase with 1X enzyme reaction buffer. 4) Add 2 μl of kinase per well of the reaction plate (prepared in step 3). 5) 1 μl of compound diluted in buffer is added per well, the plate is sealed with a plate sealer, centrifuged at 1000 g for 30 seconds, and left at room temperature for 10 minutes. 6) Prepare a 4x ATP&sub mixture with 1x enzyme reaction buffer, and add 1 μl of the 4x ATP&sub mixture to the reaction plate. 7) Seal the plate with a plate sealer, centrifuge at 1000 g for 30 seconds, and leave at room temperature for 60 minutes. 8) Transfer 4 μl of ADP-Glo to a 384 reaction plate, centrifuge at 1000 rpm / min for 1 minute, and incubate at 25° C. for 40 minutes. 9) Transfer 8 μl of detection solution to a 384 reaction plate at 1000 rpm / min, centrifuge for 1 minute, and incubate at 25° C. for 40 minutes. 10) Read the RLU (Relative luminescence unit) signal using a Biotek multifunction card reader. The signal intensity is used to represent the kinase activity.

[0299] 3. Data Analysis 3.1 The inhibition rate is calculated as follows: Inhibition rate of compound (%inh) = 100% - (compound - positive control) / (negative control - positive control) * 100%

[0300] 3.2 IC 50 is calculated and the inhibition curve of the compound is plotted. The IC of the compound was calculated using the following nonlinear fitting equation: 50(half maximal inhibitory concentration) is obtained and data analysis is performed using Graphpad 6.0 software. Y=Bottom+(Top-Bottom) / (1+10^((LogIC 50 -X)*Hill Slope)) X: logarithmic value of compound concentration Y: Inhibition rate (%inhibition)

[0301] 3.3 Validation of results Data were derived from Biotek and analyzed manually. Ratio values were converted to percent inhibition and calculated as IC 50 is calculated from Prism GraphPad 6.0 by the percentage of inhibition. IC 50 verifies the accuracy of the results by recalculating the ratio values.

[0302] 3.4 Quality Control Z factor>0.5, S / B>2. Positive control IC 50 is within three times the historical average.

[0303] 4.Results The test results are shown in Table 1 below.

[0304] [Table 12]

[0305] The compounds of the test examples all exhibit inhibitory activity against TRKA, TRKB, and TRKC kinases similar to or better than LOXO-101.

[0306] While particular embodiments of the present invention have been illustrated and described, it is not intended that these embodiments illustrate and describe all possible forms of the present application. Rather, the words used in the specification of the present invention are descriptive and not limiting. It will be apparent to those skilled in the art that various other changes and modifications can be made without departing from the general scope of the present disclosure. It is, therefore, intended in the appended claims to cover all such changes and modifications within the scope of the present invention.

Claims

1. A compound of formula (I), or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or mixture of isomers thereof, or a pharmaceutically acceptable salt thereof. 【Chemical 1】 (In the formula, R １ , R ２ are H, CN, C １－４ Alkyl, C １－４ Alkyl halides, C １－４ are independently selected from alkoxy and halogen; R ３ is H, C １－４ Alkyl, C １－４ Alkyl halides, C １－４ Alkoxy, C ３－６ selected from alicyclic groups and 4- to 6-membered heteroalicyclic groups; R ６ The number of R is 1, 2 or 3, and each R ６ is H, halogen, -CN, -OH, -NO ２ , -NR ７ R ８ , C １－４ Alkyl, C １－４ Alkyl halides, C １－４ Alkoxy, C ３－６ are independently selected from an alicyclic group, and a 4- to 6-membered heteroalicyclic group; X is a connecting bond, O, S or (NR ４ ), where R ４ is H, C １－４ Alkyl, C １－４ Alkyl halides, C １－４ Alkoxy, C ３－６ selected from alicyclic groups and 4- to 6-membered heteroalicyclic groups; R ７ and R ８ is H, C １－６ Alkyl, C １－４ Alkyl halides, C １－４ alkoxy, or R ７ and R ８ form a 3- to 6-membered ring together with the N atom to which they are attached, n=1, 2 or 3; L is (C=O), (O=S=O), CR ａ R ｂ or a connecting bond, where R ａ and R ｂ is H, C １－４ Alkyl, C １－４ Alkyl halides, C １－４ Alkoxy, C ３－６ are independently selected from an alicyclic group and a 4- to 6-membered heteroalicyclic group, or R ａ and R ｂ form a 3- to 6-membered ring together with the carbon atoms to which they are attached, R ５ is H, halogen, -OH, -NO ２ , -CN, -SF ５ , -SH, -SC １－４ Alkyl, C １－６ Alkyl, C １－６ Alkyl halides, C １－６ Alkoxy, C １－６ Halogenated alkoxy, C ２－８ Alkenyl, C ２－８ Alkynyl, C ３－７ Alicyclic group, 3- to 10-membered heteroalicyclic group, C ６－１２ Dicycloaliphatic group, 6- to 12-membered dicycloheteroalicyclic group, C ８―１５ 8-15-membered tricycloaliphatic group, 8-15-membered tricycloheteroaliphatic group, C ５－８ Aryl, 5- to 10-membered heteroaryl, C ７－１１ dicycloaryl, 7- to 11-membered dicycloheteroaryl, —C １－４ Alkyl-(C ３－７ alicyclic group), -C １－４ alkyl-(3- to 10-membered heteroalicyclic group), —C １－４ Alkyl-(C ６－１２ dicycloaliphatic group), —C １－４ alkyl-(6- to 12-membered dicycloheteroalicyclic group), —C １－４ Alkyl-(C ８―１５ -membered tricycloaliphatic group), —C １－４ alkyl-(8- to 15-membered tricycloheteroalicyclic group), —C １－４ Alkyl-(C ５－８ aryl), -C １－４ alkyl-(5- to 10-membered heteroaryl), —N(R １０ ) (R １１ ), -N(R １０ ) (C(=O)R １１ ), -N(R １０ )(C(=O)-OR １１ ), -N(R １２ )(C(=O)-N(R １０ ) (R １１ )), -C(=O)-N(R １０ ) (R １１ ), -C(=O)-R １２ , -C(=O)-OR １２ , —OC(═O)R １２ , -N(R １０ ) (S(=O) ２ R １１ ), -S(=O) ２ -N(R １０ ) (R １１ ), -SR １２ and -OR １２ wherein said -SC is selected from １－４ Alkyl, C １－６ Alkyl, C １－６ Alkoxy, C １－６ Halogenated alkoxy, C ２－８ Alkenyl, C ２－８ Alkynyl, C ３－７ Alicyclic group, 3- to 10-membered heteroalicyclic group, C ６－１２ Dicycloaliphatic group, 6- to 12-membered dicycloheteroalicyclic group, C ８―１５ 8-15-membered tricycloaliphatic group, 8-15-membered tricycloheteroaliphatic group, C ５－８ Aryl, 5- to 10-membered heteroaryl, C ７－１１ dicycloaryl, 7- to 11-membered dicycloheteroaryl, —C １－４ Alkyl-(C ３－７ alicyclic group), -C １－４ alkyl-(3- to 10-membered heteroalicyclic group), —C １－４ Alkyl-(C ６－１２ dicycloaliphatic group), —C １－４ alkyl-(6- to 12-membered dicycloheteroalicyclic group), —C １－４ Alkyl-(C ８―１５ -membered tricycloaliphatic group), —C １－４ alkyl-(8- to 15-membered tricycloheteroalicyclic group), —C １－４ Alkyl-(C ５－８ aryl) and -C １－４ Alkyl-(5- to 10-membered heteroaryl) is a 5- to 10-membered heteroaryl having 0, 1, 2, 3, or 4 R ５ａ each optionally substituted with R ５ａ is halogen, -OH, -NO ２ , -CN, -SF ５ , -SH, -SC １－４ Alkyl, oxo, C １－４ Alkyl, C １－４ Alkyl halides, C １－４ Alkoxy, C １－４ Halogenated alkoxy, C ２－８ Alkenyl, C ２－８ Alkynyl, C ３－７ Alicyclic group, 3- to 10-membered heteroalicyclic group, C ５－８ Aryl, 5- to 7-membered heteroaryl, —N(R １３ ) (R １４ ), -N(R １３ ) (C(=O)R １４ ), -N(R １３ )(C(=O)-OR １４ ), -N(R １５ )(C(=O)-N(R １３ ) (R １４ )), -C(=O)-N(R １３ ) (R １４ ), -C(=O)-R １５ , -C(=O)-OR １５ , —OC(═O)R １５ , -N(R １３ ) (S(=O) ２ R １４ ), -S(=O) ２ -N(R １３ ) (R １４ ), -SR １５ and -OR １５ are independently selected from R １０ , R １１ , R １２ , R １３ , R １４ and R １５ For each occurrence, H, C １－６ Alkyl, C １－６ Alkyl halides, C ３－７ Alicyclic group, 3- to 10-membered heteroalicyclic group, C ５－８ Aryl, 5- to 7-membered heteroaryl, C ７－１１ dicycloaryl, 7- to 11-membered dicycloheteroaryl, —C １－４ Alkyl-(C ３－７ alicyclic group), -C １－４ alkyl-(3- to 10-membered heteroalicyclic group), —C １－４ Alkyl-(C ６－１２ dicycloaliphatic group), —C １－４ alkyl-(6- to 12-membered dicycloaliphatic group), —C １－４ Alkyl-(C ８－１５ -membered tricycloaliphatic group), —C １－４ alkyl-(8- to 15-membered tricycloaliphatic group), —C １－４ Alkyl-(C ５－８ aryl) and -C １－４ alkyl-(5-10 membered heteroaryl), wherein each member within said group is selected from the group consisting of halogen, —OH, —NH ２ , -NH(CH ３ ), -N(CH ３ ) ２ , -CN, -NO ２ , -SF ５ , -SH, -SC １－４ Alkyl, oxo, C １－４ Alkyl, C ２－６ Alkenyl, C ２－６ Alkynyl, C ３－７ Alicyclic group, 3- to 10-membered heteroalicyclic group, C ５－８ Aryl, 5- to 7-membered heteroaryl, C ７－１１ Dicycloaryl, 7- to 11-membered dicycloheteroaryl, C １－４ Hydroxyalkyl, —S—C １－４ Alkyl, —C(═O)H, —C(═O)—C １－４ Alkyl, —C(═O)—O—C １－４ Alkyl, —C(═O)—NH ２ , -C(=O)-N(C １－４ alkyl) ２ , C １－４ Alkyl halides, C １－４ Alkoxy and C １－４ optionally substituted with 0, 1, 2, 3, or 4 substituents each independently selected from the group consisting of halogenated alkoxy, or R １０ and R １１ form a 3- to 14-membered ring together with the atoms to which they are connected, or R １３ and R １４ form a 3- to 14-membered ring together with the atoms to which they are connected.

2. n=1 or 2; 10. The compound of claim 1, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or mixture of isomers thereof, or a pharmaceutically acceptable salt thereof.

3. X is —O— or —NH—; 10. The compound of claim 1, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or mixture of isomers thereof, or a pharmaceutically acceptable salt thereof.

4. R １ and R ２ are each independently selected from H, F, Cl and Br; 10. A compound according to claim 1, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or mixture of isomers thereof, or a pharmaceutically acceptable salt thereof.

5. L is -(C=O)-, -(O=S=O)-, -CH ２ -, -C(CH ３ ) ２ -, -CH(CH ３ ) - or a connecting bond, 10. The compound of claim 1, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or mixture of isomers thereof, or a pharmaceutically acceptable salt thereof.

6. R ６ is H, 10. The compound of claim 1, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or mixture of isomers thereof, or a pharmaceutically acceptable salt thereof.

7. R ５ is H, halogen, -OH, -NO ２ , -CN, -SF ５ , -SH, -SC １－４ Alkyl, C １－６ Alkyl, C １－６ Alkyl halides, C １－６ Alkoxy, C １－６ Halogenated alkoxy, C ３－６ Alicyclic group, 4- to 6-membered heteroalicyclic group, C ５－８ aryl, 5- to 10-membered heteroaryl, —C １－４ Alkyl-(C ３－７ alicyclic group), -C １－４ alkyl-(3- to 10-membered heteroalicyclic group), —C １－４ Alkyl-(C ５－８ aryl), -C １－４ alkyl-(5- to 10-membered heteroaryl), —N(R １０ ) (R １１ ) wherein said -SC １－４ Alkyl, C １－６ Alkyl, C １－６ Alkoxy, C １－６ Halogenated alkoxy, C ３－６ Alicyclic group, 4- to 6-membered heteroalicyclic group, C ５－８ aryl, 5- to 10-membered heteroaryl, —C １－４ Alkyl-(C ３－７ alicyclic group), -C １－４ alkyl-(3- to 10-membered heteroalicyclic group), —C １－４ Alkyl-(C ５－８ aryl) and -C １－４ Alkyl-(5- to 10-membered heteroaryl) is a 5- to 10-membered heteroaryl having 0, 1, 2, 3, or 4 R ５ａ each optionally substituted with R ５ａ is halogen, -OH, -NO ２ , -CN, oxo, C １－４ Alkyl, C １－４ Alkyl halides, C １－４ Alkoxy, C １－４ Halogenated alkoxy, C ３－７ Alicyclic group, 3- to 10-membered heteroalicyclic group, C ５－８ independently selected from aryl and 5- to 7-membered heteroaryl; R １０ , R １１ and R １２ For each occurrence, H, C １－６ Alkyl, C １－６ Alkyl halides, C ３－７ are each independently selected from the group consisting of an alicyclic group and a 3- to 10-membered heteroalicyclic group, wherein the options within said group are halogen, —OH, —NH ２ , oxo, C １－４ Alkyl, C １－４ Hydroxyalkyl, C １－４ Alkyl halides, C １－４ Alkoxy and C １－４ optionally substituted with 0, 1, 2, 3, or 4 substituents each independently selected from the group consisting of halogenated alkoxy, or R １０ , R １１ form a 3- to 8-membered ring together with the atoms to which they are attached; 10. The compound of claim 1, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or mixture of isomers thereof, or a pharmaceutically acceptable salt thereof.

8. R ５ is selected from H, halogen, —OH, methyl, ethyl, propyl, butyl, pentyl, hexyl, methoxy, ethoxy, propoxy, butoxy, pentyloxy, hexyloxy, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, phenyl, piperazinyl, piperidyl, morpholinyl, pyrrolidinyl, pyrrolyl, pyridyl, and pyrazolyl, wherein methyl, ethyl, propyl, butyl, pentyl, hexyl, methoxy, ethoxy, propoxy, butoxy, pentyloxy, hexyloxy, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, phenyl, piperazinyl, piperidyl, morpholinyl, pyrrolidinyl, pyrrolyl, pyridyl, and pyrazolyl are each selected from one or two R ５a optionally substituted with R ５ａ is halogen, -OH, -NO ２ , -CN, oxo, C １－４ Alkyl, C １－４ Alkyl halides and C １－４ independently selected from alkoxy; 8. The compound of claim 7, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or isomeric mixture thereof, or a pharmaceutically acceptable salt thereof.

9. The compound is N-(1-(1-(3,5-difluorophenyl)ethyl)-3-(1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazol-5-amine, N-(3,5-difluorobenzyl)-3-(5-(piperidin-4-ylmethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazol-5-amine, 5-(3,5-difluorobenzyl)-3-(5-(methylsulfonyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(1-methylpiperidin-4-yl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-N,N-dimethyl-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)formamide, 2-(dimethylamino)ethyl 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate, (2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)(1-methylpiperidin-4-yl)methanone, 2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-5-(1-methylpiperidin-4-yl)-4,5,6,7-tetrahydro-3H-imidazo[4,5-c]pyridine, (S)-5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(((1-methylpiperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 1-methylpiperidin-4-yl-2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate, N-(1-(3,5-difluorophenyl)ethyl)-3-(5-(piperidin-4-ylmethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazol-5-amine, N-(1-(1-,3,5-difluorophenyl)ethyl)-3-(5-(((1-methylpiperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazol-5-amine, cyclopropyl(2-(5-(3,5-difluorobenzyl)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)methanone, 5-(3,5-difluorobenzyl)-3-(5-(1-methylpiperidin-4-yl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(((1-methylpiperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(piperidin-4-ylmethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(((1-ethylpiperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 2-(2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)-N,N-dimethylethan-1-amine, 1-(4-((2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)methyl)piperidin-1-yl)ethan-1-one, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(((1-methylpyrrolidin-3-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(2-(1-methylpiperidin-4-yl)ethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(((1-isopropylpiperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(1-(1-methylpiperidin-4-yl)ethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(piperidin-3-ylmethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(piperidin-2-ylmethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-((1-methylazetidin-3-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 3-(5-((1-cyclobutylpiperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(((1-isopropyl-4-methylpiperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, (2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)(4-methylpiperazin-1-yl)methanone, 1-methylpyrrolidin-3-yl-2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate, 1-methylazetidin-3-yl-2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate, (R)-5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(((1-methylpiperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 1-methylpiperidin-3-yl-2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazole-5(1H)-carboxylate, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(1-(1-methylazetidin-3-yl)ethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 4-(2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)-N,N-dimethylcyclohexan-1-amine, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(1-(methylsulfonyl)piperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 4-((2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)methyl)piperidine-1-carboxylic acid methyl ester, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(1-ethylpiperidin-4-yl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(2-methyl-2-azaspiro[3.3]heptan-6-yl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(7-methyl-7-azaspiro[3.5]nonan-2-yl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(1-methylpyrrolidin-3-yl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(pyrrolidin-2-ylmethyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 5-(1-(3,5-difluorophenyl)ethoxy)-3-(5-(1-ethylpiperidin-3-yl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole, 3-(2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)-N,N-dimethylcyclobutan-1-amine, 3-(2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)-N,N-dimethylcyclohexan-1-amine, 3-(2-(5-(1-(3,5-difluorophenyl)ethoxy)-1H-indazol-3-yl)-4,6-dihydropyrrolo[3,4-d]imidazol-5(1H)-yl)-N,N-dimethylcyclopentan-1-amine, 3-fluoro-5-(1-(3-(5-((1-methylpiperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indol-5-yl)oxy)ethyl)benzonitrile, 5-(1-(3,5-difluorophenyl)propoxy)-3-(5-((1-methylpiperidin-4-yl)methyl)-1,4,5,6-tetrahydropyrrolo[3,4-d]imidazol-2-yl)-1H-indazole Selected from:

10. The compound of claim 1, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or mixture of isomers thereof, or a pharmaceutically acceptable salt thereof.

10. The compound of formula (I) is a compound represented by the following formula (Ia), a compound represented by the following formula (Ib), a compound represented by the following formula (Ic), a compound represented by the following formula (IIa), or a compound represented by the following formula (IIb):

10. The compound of claim 1, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or mixture of isomers thereof, or a pharmaceutically acceptable salt thereof. 【Chemistry 2】 (Wherein, X is —O— or —(NH)—, L, n, R ３ , R ５ , R ６ is the definition in claim 1. 【Chemistry 3】 (In the formula, L, n, R ５ , R ６ is the definition in claim 1. 【Chemistry 4】 (Wherein, X is —O— or —(NH)—, n, R ３ , R ５ , R ６ , R ａ , R ｂ is the definition in claim 1. 【Chemistry 5】 (In the formula, n, R ５ , R ６ , R ａ , R ｂ is the definition in claim 1. 【Chemistry 6】 (In the formula, n, R ５ , R ６ , R ａ , R ｂ is the definition in claim 1.

11. A compound according to claim 1, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or isomeric mixture thereof, or a pharmaceutically acceptable salt thereof; one or more pharmaceutically acceptable carriers, adjuvants or excipients; A pharmaceutical composition comprising:

12. Use of a compound according to claim 1, or an isotopically labeled compound thereof, or an optical isomer, geometric isomer, tautomer or isomeric mixture thereof, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition according to claim 11, in the manufacture of a medicament for treating a disease or condition associated with a TRK kinase or NTRK gene.

13. The disease or condition associated with the TRK kinase or NTRK gene is selected from the group consisting of cancer, pain, inflammation, neurodegenerative diseases, and cell proliferation disorders.

13. The use according to claim 12.

14. The disease or condition associated with the TRK kinase or NTRK gene is selected from hepatocellular carcinoma, breast cancer, bladder cancer, colorectal cancer, melanoma, mesothelioma, lung cancer, prostate cancer, membranous cancer, pancreatic cancer, esophageal cancer, gastric cancer, lymphatic cancer, leukemia, nasopharyngeal cancer, testicular cancer, thyroid cancer, squamous cell carcinoma, glioblastoma, neuroblastoma, uterine cancer and rhabdomyosarcoma.

14. The use according to claim 13.

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