Extract manufacturing method, cosmetic manufacturing method, and oral substance manufacturing method

Abstract

To provide a method for producing an extract, in which hot-spring water is used to increase the content of useful components in the extract as compared to the conventional art, and methods for producing a cosmetic and a substance for oral use each of which contains the extract.SOLUTION: In an extraction step for adding a starting material to a solvent to extract a useful component of the starting material, hot-spring water at a pH of 5.5 to 8.5 is used as the solvent.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a method for producing an extract in which a raw material is placed in a solvent to extract useful components, and a method for producing cosmetics or oral substances containing the extract. [Background technology]

[0002] Hot spring water contains ingredients that are good for the skin, so it is not only used for bathing but is also sometimes added to cosmetics and health foods. For example, Patent Document 1 discloses cosmetics containing Moor hot spring water, or Moor hot spring water and White Crane Reishi, White Crane Reishi extract, or White Crane Reishi extract powder as main ingredients, for the purpose of enhancing skin moisturizing ability. Furthermore, Patent Document 2 discloses a basic lotion that is made by mixing alkaline hot spring water, phenoxyethanol, collagen, elastin, placenta extract, pentylene glycol, glycerin, and butylene glycol, with the aim of increasing water retention and moisturizing power, resulting in an alkaline mixture.

[0003] In addition, placenta extract extracted from the placenta, an organ unique to mammals, contains various useful components such as amino acids and enzymes. For this reason, placenta extract is used as a raw material for cosmetics, health foods, etc. Similarly, umbilical cord extract, amniotic membrane extract, sake lees extract, and bird's nest extract are also used as raw materials for cosmetics, health foods, etc. For example, Patent Document 3 discloses a food product for inhibiting gray hair, which contains placenta extract as an active ingredient. Furthermore, Patent Document 4 discloses a diet food containing placenta extract and umbilical cord extract. Furthermore, Patent Document 5 discloses a method for producing an amniotic membrane-derived material (amniotic membrane extract) that contains many components useful for anti-aging effects. Furthermore, Patent Document 6 discloses a gel cosmetic preparation containing a carboxyl group-containing anionic polymer, a compound that is hydroxypropylmethylcellulose or xanthan gum, and sake lees extract as a skin-beautifying ingredient. Furthermore, Patent Document 7 discloses a drink containing sialic acid as a bird's nest extract, in which the sialic acid is formed as an extract extracted from bird's nest by hydrolysis using an endo-type alkaline protease as a degrading enzyme, and is contained in a beverage solvent.

[0004] Furthermore, Patent Document 8 discloses food and beverage products containing 4-vinylguaiacol, and in paragraph 0045, mentions hot spring water as one type of water that can be used as an extraction solvent when extracting 4-vinylguaiacol from plants and the like. Furthermore, Patent Document 9 discloses an ABCA12 gene expression promoter containing peony extract as an active ingredient, and in paragraph 0015 lists hot spring water as one type of water that can be used as an extraction solvent for peony. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Japanese Patent Application Laid-Open No. 2000-239146 [Patent Document 2] Japanese Patent Application Laid-Open No. 2012-250960 [Patent Document 3] Japanese Patent Application Publication No. 2019-054769 [Patent Document 4] Japanese Patent Application Laid-Open No. 2006-282609 [Patent Document 5] Japanese Patent Application Publication No. 2018-188401 [Patent Document 6] Japanese Patent Application Publication No. 2019-127460 [Patent Document 7] Japanese Patent Application Publication No. 2019-129803 [Patent Document 8] Japanese Patent Application Publication No. 2020-092719 [Patent Document 9] Japanese Patent Application Publication No. 2020-138932 Summary of the Invention [Problem to be solved by the invention]

[0006] It is desirable that extracts to be incorporated into oral substances such as cosmetics or health foods contain as many useful components as possible. However, when hot spring water is mixed after extraction to form a mixture, as in Patent Documents 1 and 2, the concentration of the extract becomes diluted, and the amount of useful components contained in the mixture may not necessarily be sufficient. Furthermore, Patent Documents 3-7 do not attempt to increase the amount of useful components contained in the extract by using hot spring water. Furthermore, Patent Documents 8 and 9 state that hot spring water may be used as an extraction solvent, but this is merely one example of an extraction solvent and does not focus on hot spring water to increase the amount of useful components contained in the extract. Therefore, the present invention aims to provide a method for producing an extract that uses hot spring water to increase the content of useful ingredients in the extract (extract, extraction solution) compared to conventional methods, thereby improving its usefulness to the skin, as well as a method for producing cosmetics and oral substances that contain the extract. [Means for solving the problem]

[0007] The method for producing an extract according to claim 1 is characterized in that in the extraction step in which the raw material is placed in a solvent to extract useful components of the raw material, hot spring water having a pH of 5.5 or more and 8.5 or less is used as the solvent. The present invention according to claim 2 is characterized in that in the method for producing an extract according to claim 1, the raw material is placenta, umbilical cord, amniotic membrane, sake lees, or bird's nest. The present invention as set forth in claim 3 is characterized in that in the method for producing an extract as set forth in claim 1, the raw material is placenta, umbilical cord, or amniotic membrane, and the method includes a freezing step of freezing the raw material before the extraction step, a room temperature thawing step of thawing the raw material at room temperature after the freezing step, a washing step of washing the raw material after the room temperature thawing step, and a mincing step of shredding the raw material after the washing step. The present invention according to claim 4 is characterized in that in the method for producing an extract according to any one of claims 1 to 3, the hot spring water is Yugawara hot spring water. The present invention as set forth in claim 5 is characterized in that in the method for producing the extract as set forth in claim 4, the Yugawara hot spring water is a sodium-calcium chloride-sulfate hot spring and is weakly alkaline. A method for producing cosmetics according to claim 6 is characterized in that an extract obtained by the method for producing an extract according to any one of claims 1 to 5 is blended. A method for producing an oral substance according to claim 7 is characterized in that an extract obtained by the method for producing an extract according to any one of claims 1 to 5 is blended. [Effects of the Invention]

[0008] According to the present invention, the content of useful components in the extract can be increased compared to conventional extracts, thereby enhancing the usefulness to the skin. [Brief explanation of the drawings]

[0009] [Figure 1] 1 is a diagram showing a manufacturing process of a placenta extract (placenta extract) according to an embodiment of the present invention. [Figure 2] Graph showing the results of component analysis of the placenta extract [Figure 3] Graph showing the results of skin moisture content test 1 [Figure 4] Graph showing the results of skin moisture content test 2 [Figure 5] Graph showing the results of component analysis of the same umbilical cord extract (umbilical cord extract) [Figure 6]Graph showing the results of skin moisture content test 3 [Figure 7] Graph showing the results of component analysis of the same amniotic membrane extract (amniotic membrane extract) [Figure 8] Graph showing the results of skin moisture content test 4 [Figure 9] Graph showing the results of component analysis of the sake lees extract (sake lees extract) [Figure 10] Graph showing the results of skin moisture content test 5 DETAILED DESCRIPTION OF THE INVENTION

[0010] The methods for producing an extract, a cosmetic product, and an oral product according to embodiments of the present invention will be described below. FIG. 1 is a diagram showing the manufacturing process of placenta extract (placenta extract). As the raw material for the extract, various animal and plant-derived raw materials such as umbilical cord, amniotic membrane, sake lees, or bird's nest can be used, but here we will explain the use of placenta as a representative raw material. First, a full-term placenta is collected immediately after delivery (S1: placenta collection step). The placenta to be collected may be that of a mammal such as a human, pig, horse, or sheep, but it is preferable to use a placenta from a healthy pig or horse, which is highly safe and suitable for use in cosmetics and health foods. The collected placenta is immediately frozen (S2: first freezing step). The frozen placenta is transported to a designated location and stored. This allows the collected placenta to be stored in good condition. At the time of use, the frozen placenta is thawed at room temperature (S3: thawing step). The thawed placenta is washed after removing the membrane tissue (S4: washing step), and the chorionic tissue is shredded (S5: mincing step). In the mincing step S5, the placenta is shredded to a predetermined size using a cutting machine such as a mincer. After the mincing step S5, the placenta is promptly placed in a freezer to freeze (S6: second freezing step), which activates the useful components contained in the placenta and improves its stability.

[0011] Next, the shredded placenta is placed in a solvent for decomposition and extraction to extract useful components (S7: extraction step). Methods for decomposition and extraction include enzymatic decomposition, hydrolysis, acid treatment, and freeze-thawing, but enzymatic decomposition is particularly preferred. Hot spring water with a pH of 5.5 to 8.5 is used as the solvent. By using hot spring water with a pH of 5.5 to 8.5 as the solvent, it is possible to extract all useful components and obtain a placenta extract with a higher concentration of useful components than when using tap water or purified water as the solvent. In addition, since the pH of the hot spring water used as the solvent is 5.5 to 8.5, pH adjustment is unnecessary or easy when blending placenta into cosmetics.

[0012] The extract (placenta extract) obtained in extraction step S7 is heat sterilized (S8: heat sterilization step), and then filtered to remove impurities and concentrate the extract (S9: filtration step). After filtration step S9, preservatives are added and the extract is inspected, filled, packaged, etc. as appropriate. This allows it to be made into a liquid raw material for placenta extract to be incorporated into cosmetics, health foods, etc. Furthermore, by freeze-drying or spray-drying the placenta extract after the filtration step S9, it is possible to obtain a powdered raw material of placenta extract to be incorporated into cosmetics, health foods, etc. Furthermore, by blending a predetermined amount of the obtained liquid or powder raw material of placenta extract, it is possible to make placenta extract-containing cosmetics or oral substances such as health foods.

[0013] The hot spring water used as the solvent during extraction is preferably Yugawara hot spring water, and more preferably weakly alkaline with a pH of 7.5 or higher but lower than 8.5. Yugawara hot spring water springs from Yugawara Town in the southwestern part of Kanagawa Prefecture and the surrounding area. Yugawara hot spring water is colorless and odorless, which reduces the impact on color and odor when blended into cosmetics, health foods, etc. Below, each example in which Yugawara hot spring water was used as a solvent will be described. [Example]

[0014] Example 1 is a placenta extract produced using placenta as a raw material by the process shown in Figure 1. The method of decomposition and extraction in the extraction step S7 was an enzymatic decomposition treatment. The pH and spring quality of the Yugawara hot spring water used are as follows. Source: Miyagami, Yugawara-cho, Ashigarashimo-gun, Kanagawa Prefecture pH: 8.2 Spring quality: Sodium, calcium, chloride, sulfate hot spring (former name: gypsum-containing saline spring) The results of the component analysis of the placenta extract of Example 1 are shown in Table 1 below, along with the results of the component analysis of the placenta extracts of Comparative Examples 1 to 3. Figure 2 is a graph of the results of Table 1, where Figure 2(a) shows free amino acids, Figure 2(b) shows minerals, and Figure 2(c) shows peptide molecular weight distribution. Comparative Example 1 is a placenta extract produced in the same manner as in Example 1, except that purified water was used as the extraction solvent. Comparative Example 2 is a placenta extract produced in the same manner as in Example 1, except that hot spring water from a certain place in northern Kanto was used as the extraction solvent. The pH and spring quality of the hot spring water from a certain place in northern Kanto are as follows: - Source: Somewhere in northern Kanto pH: 2.0 Spring quality: Acidic, sulfur-containing, aluminum-sulfate, chloride hot spring (hydrogen sulfide type) Comparative Example 3 is a placenta extract produced in the same manner as in Example 1, except that hot spring water from a certain place in Saitama Prefecture was used as the extraction solvent. The pH and spring quality of the hot spring water from a certain place in Saitama Prefecture are as follows: Source: Saitama Prefecture pH: 8.9 Spring quality: Sodium chloride cold mineral spring

[0015] [Table 1]

[0016] Comparing Example 1 (solvent: Yugawara hot spring water) with Comparative Example 1 (solvent: purified water), increases were confirmed in all amino acids except glycine. Increases were confirmed in minerals, including calcium and potassium, which are expected to have beauty benefits. Regarding peptide molecular weight, the distribution of molecules with sizes of 1,000 or more decreased, while the distribution of molecules with sizes of less than 1,000, particularly less than 500, which have high penetration and moisturizing effects, increased. On the other hand, when Comparative Example 2 (solvent: hot spring water from a certain place in northern Kanto) was compared with Comparative Example 1, increases in calcium and potassium were confirmed for minerals, but decreases were observed for amino acids other than proline and cystine. Regarding peptide molecular weight, the distribution of molecules with a size of less than 500 increased, but the distribution of molecules with a size of 500 to less than 3,000 decreased, and the distribution of molecules with a size of 3,000 or more increased. Furthermore, when Comparative Example 3 (solvent: hot spring water from a certain place in Saitama Prefecture) was compared with Comparative Example 1, increases in the amino acids isoleucine, alanine, tryptophan, and cystine were confirmed, but the increases were all smaller than in Example 1. Regarding minerals, an increase in calcium was confirmed, but potassium decreased. Regarding peptide molecular weights, the distribution of molecules with a size of 3,000 or more increased, while the distribution of molecules with a size of less than 3,000 decreased. In this way, by using Yugawara hot spring water as a solvent, the content of useful ingredients in the placenta extract can be increased compared to when purified water or other hot spring water is used as a solvent, and the molecular distribution of sizes less than 1,000, particularly less than 500, can be increased.

[0017] The placenta extract of Example 1 was subjected to the following test, measuring the change in the amount of moisture increase in the skin over time after application, and confirming its effect of inhibiting evaporation of moisture from the skin. [Skin moisture content test 1] [conditions] Temperature: 25℃ Humidity: 25% Measuring equipment: Skin surface stratum corneum moisture content measuring device (Yayoi Co., Ltd. SKICON-200EX) [sample] Example 1: Placenta extract using Yugawara hot spring water as the solvent Comparative Example 1: Placenta extract using purified water as the solvent Comparative Example 4: Yugawara hot spring water Comparative Example 5: Purified water [method] 1. Use a permanent marker to mark a 3.0 cm x 2.0 cm square box on the inside of the lower arm. 2. Wash the measurement point with soap and rest for 30 minutes. 3. Measure the normal skin moisture content at the measurement point. Measure five times and calculate the average value. 4. Place 0.1g of sample on the measurement area and rub the sample evenly up to the second joint of your finger in both vertical and horizontal directions 10 times each. 5. After applying the sample, measure the skin moisture content using a measuring device 5, 10, 15, and 60 minutes later. [Skin moisture content test 2] [conditions] Temperature: 25℃ Humidity: 32% Measuring equipment: Skin surface stratum corneum moisture content measuring device (Yayoi Co., Ltd. SKICON-200EX) [sample] Example 1: Placenta extract using Yugawara hot spring water as the solvent Comparative Example 5: Purified water Comparative Example 6: A mixture of placenta extract and Yugawara hot spring water using purified water as the solvent (mixing was performed immediately before the test, the mixing ratio was 1:1) [method] The same as Skin Moisture Test 1, except that measurements were taken 5, 10, 15, 60 minutes after application of the sample, as well as 30 minutes later.

[0018] Figure 3 is a graph showing the results of Skin Moisture Content Test 1, and Figure 4 is a graph showing the results of Skin Moisture Content Test 2. The horizontal axis represents time (min) and the vertical axis represents skin moisture content (μS), with normal skin moisture content set to 0. 3, the results of Example 1 are shown by "● and solid line", the results of Comparative Example 1 are shown by "▲ and long dashed line", the results of Comparative Example 4 are shown by "X and dotted line", and the results of Comparative Example 5 are shown by "◆ and short dashed line". As a result of Skin Moisture Content Test 1, the placenta extract of Example 1 showed higher skin moisture content than each of the Comparative Examples immediately after application, and even 60 minutes after application, it showed higher skin moisture content than each of the Comparative Examples. 4, the results of Example 1 are shown by "● and solid line", the results of Comparative Example 5 are shown by "◆ and long-dashed line", and the results of Comparative Example 6 are shown by "▲ and short-dashed line". As a result of Skin Moisture Content Test 2, the placenta extract of Example 1 had a skin moisture content similar to that of the placenta extract of Comparative Example 6 until about 10 minutes after application, but the subsequent decrease in skin moisture content was more gradual than in Comparative Example 6, and even 60 minutes after application, it showed a higher skin moisture content than each comparative example.

[0019] Thus, according to the production method of the present invention, a placenta extract can be obtained that contains a larger amount of useful ingredients, has improved skin utility, and has an excellent moisturizing effect. Furthermore, even when raw materials other than placenta, such as umbilical cord, amniotic membrane, sake lees, or bird's nest, are used, the manufacturing method of the present invention can enhance the content of useful components and the moisturizing effect. The test results when the raw materials were umbilical cord, amniotic membrane, or sake lees are shown below. [Example]

[0020] Example 2 is an umbilical cord extract produced using umbilical cord as the raw material. The production method is basically the same as the placenta extract production method of Example 1, but does not include the second freezing step S6. Enzymatic hydrolysis was used as the hydrolysis and extraction method in the extraction step. The pH and spring quality of the Yugawara hot spring water used are the same as those of Example 1. The results of component analysis of the umbilical cord extract (umbilical cord extract) of Example 2 are shown in Table 2 below, along with the results of component analysis of the umbilical cord extract of Comparative Example 7. FIG. 5 is a graph of the results of Table 2, with FIG. 5(a) showing minerals and FIG. 5(b) showing polysaccharide molecular weight distribution. Comparative Example 7 is an umbilical cord extract produced in the same manner as in Example 2, except that purified water was used as the extraction solvent.

[0021] [Table 2]

[0022] Comparing Example 2 (solvent: Yugawara hot spring water) with Comparative Example 7 (solvent: purified water), a significant increase in calcium, which is expected to have beauty benefits, was confirmed. Regarding the molecular weight of polysaccharides, the distribution of molecules with a size of 10,000 or more decreased, while the distribution of molecules with a size of 1,000 or more but less than 10,000, which have high penetration and moisturizing effects, increased. In this way, by using Yugawara hot spring water as a solvent, the content of useful components in the umbilical cord extract can be increased compared to when purified water is used as a solvent.

[0023] The following test was carried out on the umbilical cord extract of Example 2, and the change in the amount of moisture increase in the skin over time after application was measured to confirm the effect of inhibiting evaporation of moisture in the skin. [Skin moisture content test 3] [conditions] Temperature: 24℃ Humidity: 31% Measuring equipment: Skin surface stratum corneum moisture content measuring device (Yayoi Co., Ltd. SKICON-200EX) [sample] Example 2: Umbilical cord extract using Yugawara hot spring water as the solvent Comparative Example 5: Purified water Comparative Example 8: A mixture of umbilical cord extract and Yugawara hot spring water using purified water as the solvent (mixed immediately before the test, mixing ratio 1:1) [method] Same as skin moisture content test 2.

[0024] Figure 6 is a graph showing the results of Skin Moisture Content Test 3. The horizontal axis represents time (min) and the vertical axis represents skin moisture content (μS), with normal skin moisture content set to 0. 6, the results of Example 2 are shown by "● and solid line," the results of Comparative Example 5 are shown by "◆ and long-dashed line," and the results of Comparative Example 8 are shown by "▲ and short-dashed line." As a result of Skin Moisture Content Test 3, the umbilical cord extract of Example 2 showed a slower decrease in skin moisture content 15 minutes after application than the other comparative examples, and even 60 minutes after application, it showed a higher skin moisture content than the other comparative examples. [Example]

[0025] Example 3 is an amniotic membrane extract produced using amniotic membrane as the raw material. The production method is basically the same as the placenta extract production method of Example 1, but does not include the second freezing step S6. The decomposition and extraction method in the extraction step was an enzymatic decomposition treatment. The pH and spring quality of the Yugawara hot spring water used were the same as those of Example 1. The results of the component analysis of the amniotic membrane extract (amniotic membrane extract) of Example 3 are shown in Table 3 below, along with the results of the component analysis of the amniotic membrane extract of Comparative Example 9. Figure 7 also shows graphs of the results of Table 3, with Figure 7(a) showing free amino acids, Figure 7(b) showing minerals, and Figure 7(c) showing peptide molecular weight distribution. Comparative Example 9 is an amniotic membrane extract produced in the same manner as in Example 3, except that purified water was used as the extraction solvent.

[0026] [Table 3]

[0027] Comparing Example 3 (solvent: Yugawara hot spring water) with Comparative Example 9 (solvent: purified water), increases were confirmed in all 18 amino acid components. Increases were confirmed in minerals such as calcium, potassium, and magnesium, which are expected to have beauty benefits. Regarding peptide molecular weight, the molecular distribution of 1,000 to 6,000 sizes significantly decreased, while the molecular distribution of less than 500 sizes, which have high penetration and moisturizing effects, increased. In this way, by using Yugawara hot spring water as a solvent, the content of useful ingredients in the amniotic membrane extract can be increased compared to when purified water is used as a solvent, and the molecular distribution of less than 500 can be increased.

[0028] The amniotic membrane extract of Example 3 was subjected to the following test, measuring the change in the amount of moisture increase in the skin over time after application, and confirming its effect of inhibiting evaporation of moisture from the skin. [Skin moisture content test 4] [conditions] Temperature: 25℃ Humidity: 41% Measuring equipment: Skin surface stratum corneum moisture content measuring device (Yayoi Co., Ltd. SKICON-200EX) [sample] Example 3: Amniotic membrane extract using Yugawara hot spring water as the solvent Comparative Example 5: Purified water Comparative Example 10: Mixture of amniotic membrane extract and Yugawara hot spring water using purified water as the solvent (mixing was performed immediately before the test, the mixing ratio was 1:1) [method] Same as skin moisture content test 2.

[0029] 8 is a graph showing the results of Skin Moisture Content Test 4. The horizontal axis represents time (min) and the vertical axis represents skin moisture content (μS), with normal skin moisture content set to 0. 8, the results of Example 3 are shown by "● and solid line", the results of Comparative Example 5 are shown by "◆ and long-dashed line", and the results of Comparative Example 10 are shown by "▲ and short-dashed line". As a result of Skin Moisture Content Test 4, the amnion extract of Example 3 showed higher skin moisture content than each of the Comparative Examples immediately after application, and even 60 minutes after application, it showed higher skin moisture content than each of the Comparative Examples. [Example]

[0030] Example 4 is a sake lees extract produced using sake lees as the raw material. The sake lees extract of Example 4 was produced by adding 1,3-butylene glycol to a solvent containing sake lees and extracting the extract over a predetermined period of time while stirring periodically. The pH and spring quality of the Yugawara hot spring water used as the solvent were the same as those of Example 1. The results of the component analysis of the sake lees extract (sake lees extract) of Example 4 are shown in Table 4 below, along with the results of the component analysis of the sake lees extract of Comparative Example 11. Also, Figure 9 is a graph of the results of Table 4, with Figure 9(a) showing amino acids, Figure 9(b) showing vitamins, and Figure 9(c) showing minerals. Comparative Example 11 is a sake lees extract produced in the same manner as in Example 4, except that purified water was used as the extraction solvent. Furthermore, sake lees is extracted using a different method than placenta-based raw materials, and because it originally contains less protein, the method for measuring amino acids is different from that used when placenta-based raw materials are used. Specifically, when using extracts made from placenta-based raw materials, the free amino acids in the extract are measured as is, whereas when using sake lees extract, all of the proteins in the extract are hydrolyzed before measuring the amino acids. Another difference is that when using extracts made from placenta-based raw materials, only those that function as amino acids are measured, whereas when using sake lees extract, all of the amino acids contained are measured, regardless of whether they function as amino acids.

[0031] [Table 4]

[0032] Comparing Example 4 (solvent: Yugawara hot spring water) with Comparative Example 11 (solvent: purified water), increases were confirmed in 11 out of 18 amino acids. Increases were confirmed in minerals such as calcium and potassium, which are expected to have beauty effects. Increases were confirmed in vitamins such as vitamin B6 and niacin, which are expected to have beauty effects. In this way, by using Yugawara hot spring water as a solvent, the content of useful components in the sake lees extract can be increased compared to when purified water is used as a solvent.

[0033] The following test was carried out on the sake lees extract of Example 4, and the change in the amount of moisture increase in the skin over time after application was measured to confirm the effect of inhibiting evaporation of moisture in the skin. [Skin moisture test 5] [conditions] Temperature: 25℃ Humidity: 31% Measuring equipment: Skin surface stratum corneum moisture content measuring device (Yayoi Co., Ltd. SKICON-200EX) [sample] Example 4: Sake lees extract using Yugawara hot spring water as the solvent Comparative Example 5: Purified water Comparative Example 11: Sake lees extract using purified water as the solvent Comparative Example 12: A mixture of sake lees extract and Yugawara hot spring water using purified water as the solvent (mixed immediately before the test, mixing ratio 1:1) [method] Same as skin moisture content test 2.

[0034] 10 is a graph showing the results of Skin Moisture Content Test 5. The horizontal axis represents time (min) and the vertical axis represents skin moisture content (μS), with normal skin moisture content set to 0. 10, the results of Example 4 are shown by "● and solid line", the results of Comparative Example 5 are shown by "◆ and short dashed line", the results of Comparative Example 11 are shown by "X and dotted line", and the results of Comparative Example 12 are shown by "▲ and long dashed line". As a result of Skin Moisture Content Test 5, the sake lees extract of Example 4 showed higher skin moisture content than each of the comparative examples immediately after application, and still showed higher skin moisture content than each of the comparative examples 60 minutes after application. [Explanation of symbols]

[0035] S1 Placenta collection process S2 First freezing process (freezing process) S3 Thawing process S4 Cleaning process S5 Mincing process S6 Second freezing process (freezing process) S7 Extraction process S8 Heat sterilization process S9 Filtration process

Claims

1. In an extraction step of extracting useful components from a raw material by putting the raw material into a solvent, hot spring water having a pH of 5.5 or more and 8.5 or less is used as the solvent; The hot spring water is Yugawara hot spring water, A method for producing an extract, wherein the raw material is placenta, umbilical cord, amniotic membrane, sake lees, or bird's nest.

2. In an extraction process in which a raw material is put into a solvent to extract useful components of the raw material, hot spring water having a pH of 5.5 or more and 8.5 or less is used as the solvent; The hot spring water is Yugawara hot spring water, the raw material is placenta, umbilical cord, or amniotic membrane; Prior to the extraction step, a freezing step of freezing the raw material; a room temperature melting step of melting the raw material at room temperature after the freezing step; a washing step of washing the raw material after the room temperature melting step; A method for producing an extract, characterized in that a mincing process for shredding the raw material is carried out after the washing process.

3. 3. The method for producing an extract according to claim 1 or 2, wherein the Yugawara hot spring water is a sodium-calcium chloride-sulfate hot spring and is weakly alkaline.

4. A method for producing cosmetics, comprising blending an extract obtained by the method for producing an extract according to any one of claims 1 to 3.

5. A method for producing an oral substance, comprising blending an extract obtained by the method for producing an extract according to any one of claims 1 to 3.

Images