Cinnamon extract-containing preparation

Abstract

To provide an active ingredient of cosmetic which can enhance firmness and suppleness of skin.SOLUTION: A proliferation promoter of a noradrenaline receptor and / or an oxytocin receptor which contains cinnamon bark extract. An external preparation for skin for increasing push-back force of skin which contains cinnamon bark extract.EFFECT: Sensibility to noradrenaline and / or oxytocin is increased in a fibroblast, and production of tenascin X is promoted into skin having strong force of pushing back from inside.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to a novel use of coffee extract and to a preparation containing coffee extract.

Background Art

[0002] Skin aging symptoms such as wrinkles and sagging have a great impact on the visual impression, so there is high interest in improving them. In recent years, the development of anti-aging agents, anti-wrinkle agents, moisturizers, etc. for the purpose of improving skin aging symptoms has been actively carried out, and various cosmetics for obtaining firm and elastic skin have been proposed (Patent Documents 1 to 3, etc.).

[0003] In the dermis and subcutaneous fibrous structure, tenascin X, which is one of the glycoproteins, exists. It connects collagen fibers, forms the interval of the extracellular matrix, and enhances the compressive stress of the skin (Non-Patent Documents 1 to 3, etc.). Tenascin X is known to be secreted from fibroblasts (Non-Patent Document 4). So far, there is no example of targeting tenascin X as an anti-aging agent.

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Patent Document 2

Patent Document 3

Patent Document 4

Non-Patent Documents

[0005]

Non-Patent Document 1

Non-Patent Document 2

[0006] Firm and elastic skin gives a youthful and healthy appearance, so there is a high demand for cosmetics that provide such effects. However, conventional anti-aging cosmetics sometimes do not provide sufficient results, and there is a need for more effective ingredients. This invention was made under such circumstances, and aims to provide an active ingredient for cosmetics that can enhance firmness and elasticity (compressive stress) that pushes back from within the skin. [Means for solving the problem]

[0007] The inventors discovered that touching skin with high compressive stress increases curiosity. Therefore, they focused on the effect of tenascin X on increasing the compressive stress of skin and conceived that a component that promotes its production would be suitable as an active ingredient in cosmetics to enhance the skin's resilience. After diligent efforts, they discovered that tenascin X increases the amount of neurotransmitters such as norepinephrine and oxytocin that bind to fibroblasts, which are likely to be secreted when curiosity increases, and thus increases their production. Furthermore, they discovered that cinnamon extract can increase norepinephrine receptors and oxytocin receptors in fibroblasts, thereby increasing the sensitivity of fibroblasts to norepinephrine and oxytocin, thus completing the present invention.

[0008] In other words, the present invention relates to novel uses of cinnamon extract, and specifically to the following: [1] A proliferative agent for noradrenaline receptors and / or oxytocin receptors containing cinnamon extract. [2] A tenascin X production promoter containing cinnamon extract. [3] The tenascin X production promoter according to [2], further comprising a norepinephrine secretion promoter and / or an oxytocin secretion promoter. [4] A topical skin preparation containing cinnamon extract to enhance the skin's resilience. [5] A cosmetic, as described in [4]. [Effects of the Invention]

[0009] The present invention provides a noradrenaline receptor and / or oxytocin receptor proliferation promoter that can increase the sensitivity of fibroblasts to noradrenaline and / or oxytocin. Furthermore, the present invention provides an active ingredient for cosmetics that can promote the production of tenascin X by increasing the aforementioned sensitivity, thereby leading to skin with strong resilience from within. [Brief explanation of the drawing]

[0010] [Figure 1] A graph showing the relative expression levels of noradrenaline receptors (ADRB2) in fibroblasts treated with cinnamon extract. [Figure 2] A graph showing the relative expression levels of oxytocin receptors (OXTR) in fibroblasts treated with cinnamon extract. [Figure 3] A graph showing the relative expression levels of tenascin X in fibroblasts to which the test sample was added. [Figure 4] A graph showing the relative resilience of skin after continuous use of topical skin preparations containing cinnamon extract. [Figure 5] This graph shows the relative changes in scores from a multifaceted emotional state scale questionnaire administered to subjects who continuously used a topical skin preparation containing cinnamon extract. [Figure 6] A graph showing the average VAS response values ​​from a questionnaire given to subjects who pressed on artificial skin (left bar graph: artificial skin A, right bar graph: artificial skin B). [Figure 7] Graph showing the average VAS response values from a multi-faceted emotional scale questionnaire for subjects who pressed the simulated skin (left of each bar graph: simulated skin A, right: simulated skin B).

BEST MODE FOR CARRYING OUT THE INVENTION

[0011] The cinnamon extract in the present invention refers to the general name of extracts from the bark of the plant Cinnamomum cassia of the Lauraceae family, fractions of the extract, purified fractions, and solvent-removed products thereof. As the extraction solvent, one or more selected from polar solvents such as water, alcohols such as ethanol, isopropyl alcohol, and butanol, polyhydric alcohols such as 1,3-butanediol and polypropylene glycol, ketones such as acetone and methyl ethyl ketone, and ethers such as diethyl ether and tetrahydrofuran can be exemplified as suitable ones. As a specific extraction method, for example, for 1 part by mass of a part used for extraction of a plant body or its dried product that has been previously pulverized or finely cut, 1 to 30 parts by mass of a solvent is added, immersed at room temperature for several days or at a temperature near the boiling point for several hours, cooled to room temperature, and then, if desired, insoluble substances and / or the solvent are removed, and fractionation and purification are carried out by column chromatography or the like.

[0012] Normally, noradrenaline receptors and / or oxytocin receptors are present in fibroblasts, and the cinnamon extract has the effect of promoting the growth of these receptors. Here, the growth of the receptors in fibroblasts can be confirmed by measuring the amount of the receptor protein and the expression level of the gene encoding the protein by conventional methods, or by measuring the sensitivity of noradrenaline and / or oxytocin in fibroblasts. be done. <0000​​Cinnamon extract increases the sensitivity of fibroblasts to norepinephrine and oxytocin by proliferating norepinephrine and oxytocin receptors in fibroblasts. Since norepinephrine and oxytocin are normally present in the dermis, this leads to an increase in tenascin X production. In other words, cinnamon extract indirectly functions as a tenascin X production promoter. This increase in tenascin X production can be achieved through improved sensitivity due to increased receptors, even if the levels of norepinephrine and oxytocin are not necessarily high. Furthermore, the inventors have confirmed that in the absence of cinnamon extract, tenascin X production increases in a manner dependent on the levels of norepinephrine and / or oxytocin. Because tenascin X is responsible for compressive stress in the dermis and subcutaneous fibrous structure, topical skin preparations containing cinnamon extract have the effect of enhancing firmness and elasticity, especially the ability to push back from within.

[0013] Furthermore, as shown in the test examples described later, it has been found that touching skin that has a resistance from within increases curiosity and leads to a more positive psychological state. The agent of the present invention is also useful because this feeling of exhilaration leads to the secretion of norepinephrine and oxytocin, creating a positive feedback loop that increases the production of tenascin X in fibroblasts.

[0014] In a tenascin X production promoter containing cinnamon extract, it is preferable to further contain a norepinephrine secretion promoter and / or an oxytocin secretion promoter. This is because tenascin X production is promoted by the binding of norepinephrine and oxytocin to receptors on fibroblasts, and therefore, an increase in the amount of norepinephrine and oxytocin further enhances the tenascin X production effect. Examples of norepinephrine secretion promoters include mirtazapine. Examples of oxytocin secretion-promoting agents include PrRP (19P2-L31). Furthermore, the terms "norepinephrine secretion promoter" and "oxytocin secretion promoter" used here are not limited to pharmaceutical or other medical ingredients; any ingredient capable of promoting the secretion of norepinephrine or oxytocin is acceptable. In addition, ingredients, textures, and fragrances that can enhance (increase) active emotions involving norepinephrine and / or mythical emotions involving oxytocin are also preferable for use in combination with cinnamon extract.

[0015] When the agent of the present invention is included in a topical skin preparation, the amount of cinnamon extract is preferably 0.00001% by mass or more, more preferably 0.001% by mass or more, even more preferably 0.1% by mass or more, and also preferably 15% by mass or less, more preferably 10% by mass or less, and even more preferably 5% by mass or less, relative to the total amount of the topical skin preparation. By defining the range in this way, it becomes easier to obtain the desired effect and to ensure a degree of freedom in formula design.

[0016] The dosage forms of the topical skin preparation of the present invention include, but are not limited to, lotion forms, emulsifier forms such as emulsions and creams, oil forms, oil gel forms, gel forms, packs, cleansing agents, and the like. Furthermore, the topical skin preparation of the present invention is suitable for use as a cosmetic and a quasi-drug.

[0017] In the topical skin preparation of the present invention, in addition to cinnamon extract, any other components commonly used in topical skin preparations may be included, as long as they do not impair the effects of the present invention. Examples of such optional components include various active ingredients, oily components, surfactants, polyhydric alcohols, thickeners, powders, and ultraviolet absorbers.

[0018] Active ingredients include skin whitening ingredients, wrinkle-improving ingredients, anti-inflammatory ingredients, and extracts derived from other plants and animals. As for whitening ingredients, there are no particular limitations as long as they are commonly used in cosmetics. For example, 4-n-butylresorcinol, ascorbic acid glucoside, 3-O-ethyl ascorbic acid, arbutin, ellagic acid, kojic acid, linoleic acid, nicotinamide, 5,5'-dipropylbiphenyl-2,2'-diol, 5'-disodium adenylate, 4- Examples include potassium toxicasalicylate, hydroquinone, and pantothenic acid.

[0019] As for wrinkle-improving ingredients, there are no particular limitations as long as they are commonly used in cosmetics. Examples include sodium trifluoride isopropyl oxopropylaminocarbonylpyrrolidinecarbonylmethylpropylaminocarbonylbenzoylaminoacetate, nicotinamide, vitamin A or its derivatives (retinol, retinal, retinoic acid, tretinoin, isotretinoin, tocopherol retinoic acid, retinyl palmitate, retinyl acetate, etc.), benzyl ursolate, ursolic acid phosphate, benzyl betulinate, and benzyl acid phosphate.

[0020] Examples of anti-inflammatory components include glycyrrhizic acid, glycyrrhetinic acid, allantoin, isopropylmethylphenol, clarinon, glabridin, salicylic acid, tocopherol acetate, tocopherol nicotinate, nicotinamide, pantothenic acid, pantothenyl alcohol, and salts or derivatives thereof. Preferably, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts, pantothenyl alcohol, and pantothenic acid and its salts.

[0021] Other plant and animal-derived extracts are not particularly limited as long as they are commonly used in cosmetics, etc. For example, Akebia extract, Thujopsis dolabrata extract, Asparagus extract, Avocado extract, Hydrangea serrata extract, Almond extract, Arnica extract, Aloe extract, Aronia extract, Apricot extract, Ginkgo biloba extract, Indian kinosum extract, Fennel extract, Aralia cordata extract, Rosehip extract, Eleutherococcus senticosus extract, Enmeisou extract, Scutellaria baicalensis extract, Phellodendron amurense extract, Coptis japonica extract, Panax ginseng extract, Hypericum perforatum extract, Lamium album extract, Orange extract, Arctium moniliforme extract, Pueraria lobata extract, Chamomile extract, Carrot extract Artemisia capillaris extract, kiwi extract, cucumber extract, guava extract, Sophora flavescens extract, gardenia extract, Sasa veitchii extract, Sophora flavescens extract, walnut extract, grapefruit extract, black rice extract, chlorella extract, mulberry extract, Alpinia speciosa extract, Alpinia zerumbet extract, gentian extract, Geranium thunbergii extract, black tea extract, burdock extract, rice extract, rice fermentation extract, rice bran fermentation extract, rice germ oil, lingonberry extract, sage extract, soapwort extract, bamboo extract, sunflower extract, Japanese pepper extract, cypress extract, cypress extract, cypress extract Mushroom extract, Rehmannia glutinosa extract, Lithospermum erythrorhizon extract, Perilla extract, Linden extract, Spiraea japonica extract, Peony extract, Ginger extract, Calamus root extract, Birch extract, Horsetail extract, Stevia extract, Stevia ferment, Hedera helix extract, Crataegus monogyna extract, Elderflower extract, Yarrow extract, Peppermint extract, Sage extract, Malva sylvestris extract, Cnidium officinale extract, Swertia japonica extract, Morus alba bark extract, Rhubarb extract, Soybean extract, Jujube extract, Thyme extract, Ta Dandelion extract, tea extract, clove extract, citrus peel extract, sweet tea extract, chili pepper extract, angelica extract, calendula extract, peach kernel extract, spruce extract, Houttuynia cordata extract, tomato extract, natto extract, carrot extract, garlic extract, wild rose extract, hibiscus extract, Ophiopogon extract, lotus extract, parsley extract, birch extract, witch hazel extract, Isodon japonicus extract, cypress extract, loquat extract, coltsfoot extract, butterbur extract, Poria cocos extract, butcher's broom extract, grape extract,Grape seed extract, loofah extract, safflower extract, peppermint extract, linden extract, peony extract, hop extract, pine extract, marjoram extract, horse chestnut extract, skunk cabbage extract, soapberry extract, lemon balm extract, seaweed extract, peach extract, cornflower extract, eucalyptus extract, saxifrage extract, yuzu extract, lily extract, coix seed extract, Extracts such as mugwort extract, lavender extract, green tea extract, apple extract, rooibos tea extract, reishi mushroom extract, lettuce extract, lemon extract, forsythia extract, astragalus extract, rose extract, rosemary extract, Roman chamomile extract, royal jelly extract, and burnet extract are preferred.

[0022] Examples of oily components include polar oils and volatile hydrocarbon oils. As polar oils, synthetic ester oils include isopropyl myristate, cetyl octanoate, octyldodecyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, decyl oleate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, lanolin acetate, 2-ethylhexyl isononanoate, isocetyl stearate, isocetyl isostearate, cholesteryl 12-hydroxystearate, and di-2-ethylhexanoate. Examples include ethylene glycol, dipentaerythritol fatty acid ester, N-alkyl glycol monoisostearate, neopentyl glycol dicaprate, diisostearyl malate, glyceryl di-2-heptylundecanoate, trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, pentaneerythritol tetra-2-ethylhexanoate, glyceryl tri-2-ethylhexanoate, and trimethylolpropane triisostearate.

[0023] Furthermore, other examples include cetyl 2-ethylhexanoate, 2-ethylhexyl palmitate, glyceryl trimyristate, glyceride tri-2-heptylundecanoate, methyl castor oil fatty acid ester, oleic acid oil, cetostearyl alcohol, acetoglyceride, 2-heptylundecyl palmitate, diisobutyl adipate, 2-octyldodecyl N-lauroyl-L-glutamate, di-2-heptylundecyl adipate, ethyl laurate, di-2-ethylhexyl sebatate, 2-hexyldecyl myristate, 2-hexyldecyl palmitate, 2-hexyldecyl adipate, diisopropyl sebatate, 2-ethylhexyl succinate, ethyl acetate, butyl acetate, amyl acetate, triethyl citrate, octyl methoxycinnamate, and the like.

[0024] Other examples of natural oils include avocado oil, camellia oil, turtle oil, macadamia nut oil, corn oil, mink oil, olive oil, rapeseed oil, egg yolk oil, sesame oil, peach oil, wheat germ oil, sasanqua oil, castor oil, linseed oil, safflower oil, cottonseed oil, elm oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, cinnamon oil, Japanese tuni oil, jojoba oil, wheat germ oil, triglycerin, glyceryl trioctanoate, and glyceryl triisopalmitate.

[0025] Examples of volatile hydrocarbon oils include isododecane and isohexadecane.

[0026] The surfactants include fatty acid soaps (sodium laurate, sodium palmitate, etc.), anionic surfactants such as potassium lauryl sulfate and alkyl sulfate triethanolamine ether, cationic surfactants such as stearyltrimethylammonium chloride, benzalkonium chloride and laurylamine oxide, betaine-based surfactants (alkyl betaine, amide betaine, sulfobetaine, etc.), imidazoline-based amphoteric surfactants (2-cocoyl-2-imidazolinium hydroxide-1-carboxyethyloxy disodium salt, etc.), amphoteric surfactants such as acylmethyl taurine, sorbitan fatty acid esters (sorbitan monostearate, sorbitan sesquioleate, etc.), and glycerin fatty acid esters. Glyceryl monostearate, propylene glycol fatty acid esters (propylene glycol monostearate, etc.), hydrogenated castor oil derivatives, glycerin alkyl ethers, POE sorbitan fatty acid esters (POE sorbitan monooleate, polyoxyethylene sorbitan monostearate, etc.), POE sorbitan fatty acid esters (PO Examples include E-sorbitol monolaurate, POE glycerin fatty acid esters (POE-glycerin monoisostearate, etc.), POE fatty acid esters (polyethylene glycol monooleate, POE distearate, etc.), POE alkyl ethers (POE 2-octyldodecyl ether, etc.), POE alkylphenyl ethers (POE nonylphenyl ether, etc.), pluronic types, POE-POP alkyl ethers (POE-POP 2-decyltetradecyl ether, etc.), tetronic types, POE castor oil and hydrogenated castor oil derivatives (POE castor oil, POE hydrogenated castor oil, etc.), sucrose fatty acid esters, alkyl glucosides, and other nonionic surfactants.

[0027] Examples of polyhydric alcohols include polyethylene glycol, glycerin, 1,3-butylene glycol, erythritol, sorbitol, xylitol, maltitol, propylene glycol, dipropylene glycol, diglycerin, isoprene glycol, 1,2-pentanediol, 2,4-hexylene glycol, 1,2-hexanediol, and 1,2-octanediol.

[0028] Examples of thickening agents include guar gum, quince seed, carrageenan, galactan, gum arabic, pectin, mannan, starch, xanthan gum, curdlan, methylcellulose, hydroxyethylcellulose, carboxymethylcellulose, methylhydroxypropylcellulose, chondroitin sulfate, dermatan sulfate, glycogen, heparan sulfate, hyaluronic acid, sodium hyaluronate, tragacanth gum, keratan sulfate, chondroitin, mucoitin sulfate, hydroxyethyl guar gum, carboxymethyl guar gum, dextran, kerato sulfate, locust bean gum, succinoglycan, carotenoid acid, chitin, chitosan, carboxymethyl chitin, agar, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, sodium polyacrylate, polyethylene glycol, and bentonite.

[0029] Powders include powders such as mica, talc, kaolin, synthetic mica, calcium carbonate, magnesium carbonate, anhydrous silicic acid (silica), aluminum oxide, and barium sulfate, which may have surface treatments; inorganic pigments such as red iron oxide, yellow iron oxide, black iron oxide, cobalt oxide, ultramarine, Prussian blue, titanium dioxide, and zinc oxide, which may have surface treatments; pearlescent agents such as titanium mica, fish scale foil, and bismuth oxychloride, which may have surface treatments; and Examples include organic dyes such as Red 202, Red 228, Red 226, Yellow 4, Blue 404, Yellow 5, Red 505, Red 230, Red 223, Orange 201, Red 213, Yellow 204, Yellow 203, Blue 1, Green 201, Violet 201, and Red 204, as well as organic powders such as polyethylene powder, polymethyl methacrylate, nylon powder, and organopolysiloxane elastomers.

[0030] Examples of UV absorbers include para-aminobenzoic acid-based UV absorbers, anthranilic acid-based UV absorbers, salicylic acid-based UV absorbers, cinnamic acid-based UV absorbers, benzophenone-based UV absorbers, sugar-based UV absorbers, and 2-(2'-hydroxy-5'-t-octylphenyl)benzo UV absorbers such as triazole and 4-methoxy-4'-t-butyldibenzoylmethane Examples include, etc.

[0031] The topical skin preparation of the present invention can be manufactured by processing the essential components, preferred components, optional components, etc., according to conventional methods. [Examples]

[0032] The present invention will be described in more detail below with reference to examples, but it goes without saying that the present invention is not limited to these examples.

[0033] <1> Confirmation of the proliferative effect of norepinephrine receptors and / or oxytocin receptors. Using DMEM medium (SIGMA), normal human dermal fibroblasts were placed in a 24-well plate in quantities of 8.0 × 10⁶. 4Cells were seeded per well and cultured for 24 hours at 37°C in a 5% CO2 environment. After culturing, the culture medium was... After removing the residue and washing the cells with PBS, add DMEM medium containing cinnamon extract or the solvent, and 3 The samples were incubated for 24 hours at 7°C and 5% CO2. mRNA from the above fibroblasts was extracted using the RNeasy Mini Kit (QIAGEN), and cDNA was synthesized using the Superscript VILO DNA synthesis Kit (Lifetechnologies). Using cDNA as a template, the mRNA expression levels of the norepinephrine receptor (ADRB2) were measured using primers (primer No. R1102D01, primer No. R1102D02, Invitrogen), and the mRNA expression levels of the oxytocin receptor (OXTR) were measured using primers (primer No. R0891A02, primer No. R0891A03, Invitrogen), both by real-time qPCR. The mRNA expression level of the endogenous control gene TBP was also measured in the same manner. The measurement was performed using the QuantiFact SYBR GREEN PCR kit (manufactured by QIAGEN).

[0034] The norepinephrine receptor mRNA expression levels and oxytocin receptor mRNA expression levels in the cinnamon extract-added group and the solvent control group were corrected by the TBP expression level. The relative expression level was calculated with the expression level of the control group set to 1. The results are shown in Figures 1 and 2. Cinnamon extract was found to promote the gene expression of noradrenaline receptors and oxytocin receptors.

[0035] <2> Confirmation of tenascin X production activity Normal human dermal fibroblasts were placed in 8.0 × 10⁶ well plates using DMEM medium (SIGMA). 4 Cells were seeded per well and cultured for 24 hours at 37°C in a 5% CO2 environment. After culturing, the culture medium was... After removing the residue and washing the cells with PBS, add DMEM medium containing cinnamon extract or the solvent, and 3 The cells were cultured for 24 hours at 7°C and 5% CO2. After culturing, the culture medium was removed and the cells were fermented in PBS. After washing, 2% FBS-containing DMEM medium was added, and the cells were incubated at 37°C and 5% CO2 for 6 hours. After incubation, add norepinephrine (25304-31, Nacalai Tesque) at 10 pM and keep at 37°C. The cells were cultured for 24 hours under a 5% CO2 environment. After culturing, 0.1 pM of oxytocin (4048-V, manufactured by Peptide Research Institute Co., Ltd.) was added, and the cells were cultured for another 24 hours at 37°C under a 5% CO2 environment. mRNA from the above fibroblasts was extracted using the RNeasy Mini Kit (QIAGEN), and cDNA was synthesized using the Superscript VILO DNA synthesis Kit (Lifetechnologies). Using cDNA as a template and primers (primer No. 84695859 R2432 (G12), Primer No. 84695859 R2432 (H01), Invitrogen), the mRNA expression level of tenascin X was measured by real-time qPCR. In addition, the mRNA expression level of the endogenous control gene GAPDH was measured. mRNA expression levels were similarly measured. The measurement was performed using the QuantiFact SYBR GREEN PCR kit (QIAGEN). (Manufactured using)

[0036] mRNA expression of tenascin X in the solvent control group, the group with cinnamon extract only, the group with norepinephrine and oxytocin, and the group with norepinephrine, oxytocin, and cinnamon extract. For each quantity, correction was performed based on the GAPDH expression level, and the relative expression level was calculated with the expression level of the solvent control group set to 1. The results are shown in Figure 3. No promotion of tenascin X production was observed in the presence of norepinephrine and oxytocin, or in the presence of cinnamon extract alone. However, in the presence of norepinephrine, oxytocin, and cinnamon extract, a gene expression-promoting effect on tenascin X was observed.

[0037] <3> Confirmation of the skin's resilience and its impact on emotions by topical skin preparations containing cinnamon extract. Forty-one women in their 40s and 50s were given a topical skin preparation containing cinnamon extract, as shown in Table 1, to use continuously for three months. The effects on their skin's resilience and their emotional state were then examined.

[0038] [Table 1]

[0039] Skin elasticity was measured on the cheek area using a ballistometer (DIA-SRON), and the rebound time (the time it takes for the skin surface to return to its original position after sinking due to the pendulum falling) was used as an indicator. The relative values ​​for month 1 and month 3 were calculated, with the value at month 0 set as 1. The results are shown in Figure 4. Compared to month 0, it was observed that using a topical skin preparation containing cinnamon extract for 1 month or 3 months improved the skin's resilience.

[0040] The impact on emotions was evaluated using a multifaceted emotional state scale questionnaire. Specifically, emotional terms associated with the factors "depression / anxiety," "lethargy," "inactivity pleasure," "concentration," "hostility," "activity pleasure," "affiliation," and "shock" were randomly arranged, and participants were asked to rate each mood state on a 4-point scale from 1 to 4. For each mood state, the numerical values ​​at month 0 and month 3 were calculated. The results are shown in Figure 5. Compared to month 0, 3 months of use of a topical skin preparation containing cinnamon extract resulted in improvements in mood states related to "active well-being," which involves norepinephrine, and "affiliation," which involves oxytocin.

[0041] <Reference 1> Examination of the impact on emotions when touching skin with strong resistance Twenty women aged 20-50 were asked to select from several types of artificial skin with varying degrees of resistance to push back, choosing one that they imagined as their own skin (artificial skin A) and another that they imagined as their own skin with increased firmness (artificial skin B). In all cases, artificial skin B was selected because it had a stronger resistance to push back than artificial skin A. Participants pressed the selected artificial skin with their hands and responded to the degree of each presented emotional state using a Visual Analog Scale (VAS). First, they pressed artificial skin A and responded... After receiving the sample, participants were asked to respond after pressing on simulated skin B, which offered stronger resistance than simulated skin A.

[0042] The results are shown in Figures 6 and 7. Touching my own skin, which I imagined to be very resilient, significantly increased my positive emotions. In particular, a significant increase was observed in 4 out of 6 items related to diffusive curiosity.

[0043] <Reference 2> Examination of the effects on brain activity areas when touching skin with strong resistance Five women were asked to choose from several types of artificial skin with varying degrees of resistance, and imagine what they would like to be their own skin. Participants were asked to choose between artificial skin A, which felt firm, and artificial skin B, which they imagined to be their own skin with increased firmness. In all cases, artificial skin B was chosen because it felt stronger to the touch than artificial skin A. Brain imaging data was acquired using fMRI while touching artificial skin A with the left hand, and then, after a break, brain imaging data was acquired using fMRI with artificial skin B, which felt stronger to the touch than artificial skin A. By comparing brain image data obtained when each artificial skin was pressed, we visualized and evaluated brain activity regions that showed differences in activity.

[0044] Table 2 shows the correspondence between brain activity areas and associated functions and emotions, as well as the brain activity areas that were activated when pressing artificial skin B compared to artificial skin A, as determined by the test. When touching my own skin, which I imagined to be quite resilient, I observed activation in brain activity areas associated with curiosity and motivation.

[0045] [Table 2] [Industrial applicability]

[0046] This invention can be applied to topical skin preparations such as anti-aging cosmetics.

Claims

1. A gene expression promoter for noradrenaline receptors and / or oxytocin receptors, containing cinnamon extract.

2. A tenascin X production promoter containing cinnamon extract.

3. A tenascin X production promoter containing cinnamon extract and a norepinephrine secretion promoter and / or an oxytocin secretion promoter.

4. A topical skin preparation containing the agent described in any one of claims 1 to 3, for promoting gene expression of noradrenaline receptors and / or oxytocin receptors, or for promoting the production of tenascin X.

5. A cosmetic preparation for external use of the skin according to claim 4.

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