Topical skin preparations

Abstract

To provide a topical skin preparation that is made from a natural product and has excellent biological safety and comprises a novel active ingredient to give moisture to skin and protect it from drying.SOLUTION: A topical skin preparation comprises, as an active ingredient, a mixture of fermented seeds of Coix plants, extract from Salicornia plants and extract from Aspalathus plants.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to an external preparation for skin that increases amino acids in the stratum corneum and exhibits a skin moisturizing function.

Background Art

[0002] Conventionally, it has been known that due to causes such as aging, the natural moisturizing factor (NMF) of the skin decreases, and as a result, the skin becomes less moist and dryness occurs. Conventionally, various moisturizing agents (such as glycerin, polyhydric alcohols, sodium lactate, etc.) have been proposed to improve skin moisture and ameliorate dryness. In recent years, however, components derived from natural products, which are highly safe and highly effective, have been demanded.

Disclosure of the Invention

Problems to be Solved by the Invention

[0003] In view of the problems of such conventional technologies, the present inventors diligently conducted research to find a new active ingredient derived from natural products from the viewpoint of skin safety. As a result, they found that an active ingredient combining the fermented product of adlay seeds, the extract of Acinos arvensis, and the extract of Asparagus linearis exhibits a remarkable effect of promoting the gene expression of natural moisturizing factor (NMF)-related proteins, gives moisture to the skin, and prevents and ameliorates dryness.

[0004] Conventionally, it has been disclosed in, for example, Patent Documents 1 to 3 that each of the fermented product of adlay seeds, the extract of Acinos arvensis, and the extract of Asparagus linearis has skin physiological activity. However, it has not been known that an active ingredient combining the extracts of these plants exhibits a remarkable effect of promoting the gene expression of natural moisturizing factor (NMF)-related proteins.

Patent Document 1

Patent Document 2

Patent Document 3

Means for Solving the Problems

[0005] The present invention is a filaggrin gene expression promoter containing a mixture of fermented Job's tears seeds, glasswort extract, and Aspalathus linearis extract (solid content concentration ratio of fermented Job's tears seeds: glasswort extract: Aspalathus linearis extract = 5.5~6.0:3.0~3.5:1) as an active ingredient. Furthermore, the present invention is a peptidylarginine deiminase-1 (PADI-1) gene expression promoter containing a mixture of fermented adlay seeds, glasswort extract, and aspalathus linearis extract (solid content concentration ratio of adlay seeds ferment: glasswort extract: aspalathus linearis extract = 5.5~6.0:3.0~3.5:1) as an active ingredient. Furthermore, the present invention is a peptidylarginine deiminase-3 (PADI-3) gene expression promoter containing a mixture of fermented adlay seeds, glasswort extract, and aspalathus linearis extract (solid content concentration ratio of adlay seeds ferment: glasswort extract: aspalathus linearis extract = 5.5~6.0:3.0~3.5:1) as an active ingredient. Furthermore, the present invention relates to a topical skin preparation comprising any of the above-mentioned filaggrin gene expression promoter, peptidylarginine deiminase-1 (PADI-1) gene expression promoter, and peptidylarginine deiminase-3 (PADI-3) gene expression promoter. [Effects of the Invention]

[0006] The present invention provides a topical skin preparation that moisturizes the skin and prevents and improves dryness, based on its effect of promoting the gene expression of natural moisturizing factor (NMF)-related proteins, by using a mixture of fermented Job's tears seeds, glasswort extract, and Aspalathus linearis extract as active ingredients. [Brief explanation of the drawing]

[0007] [Figure 1] This figure shows the filaggrin gene expression promoting effect of the active ingredient according to the present invention. [Figure 2]This figure shows the effect of the active ingredient according to the present invention on promoting peptidylarginine deiminase-1 (PADI-1) gene expression. [Figure 3] This figure shows the effect of the active ingredient according to the present invention on promoting peptidylarginine deiminase-3 (PADI-3) gene expression. [Modes for carrying out the invention]

[0008] In this invention, "Job's tears" refers to a plant of the genus Coix in the grass family, and any species may be used. The part used for fermentation is preferably the seeds, and both seeds with and without hulls can be used. Furthermore, it may be the whole grain, a powder obtained by crushing or shredding, or a product of high temperature and high pressure treatment of Job's tears seeds or powder.

[0009] For fermenting Job's tears, yeast is preferred. Preferred yeasts include those of the genus Saccharomyces, such as Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, and Saccharomyces bayonus.

[0010] The Salicornia used in this invention refers to a plant belonging to the genus Salicornia in the family Chenopodiaceae, and is sometimes called coral grass. Examples include Salicornia europaea, Salicornia bigelov, and Salicornia dolicostachya ssp. strictissima. Furthermore, the whole plant is preferred as the part used for the extract.

[0011] The Aspalathus linearis used as the raw material for the Aspalathus linearis extract in this invention is a plant belonging to the genus Aspalathus of the family Fabaceae, and it is preferable to use an unfermented, green variety (so-called green rooibos). Furthermore, it is preferable to use the whole plant or leaves for the extract.

[0012] To prepare the extract, first, the usable parts of each plant are washed with water if necessary to remove foreign matter. Then, they are either left as is or dried, and if necessary, finely chopped or pulverized, and then brought into contact with the extraction solvent for extraction. Extraction can be carried out by contacting the extraction solvent according to conventional methods such as immersion.

[0013] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform. These can be used individually or in combination of two or more.

[0014] Among the extraction solvents mentioned above, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention, from the viewpoint of the effectiveness of the resulting extract mixture, as well as its low skin irritancy, and its broad applicability to topical skin preparations (cosmetics, quasi-drugs, etc.). Preferred examples of using these hydrophilic solvents include, for example, the use of water, lower alcohols (especially ethanol), or polyhydric alcohols (especially 1,3-butylene glycol) alone, or a mixed solvent of water and lower alcohols (especially ethanol), or a mixed solvent of water and polyhydric alcohols (especially 1,3-butylene glycol, glycerin), but among these, water alone or a mixed solvent of water and 1,3-butylene glycol are particularly preferred.

[0015] When using a mixed solvent, for example, in the case of a mixed solvent of water and 1,3-butylene glycol, the mixing ratio is preferably in the range of 1:1 to 20:1 by volume ratio (the same hereinafter), in the case of a mixed solvent of water and ethanol, it is 1:1 to 25:1, and in the case of a mixed solvent of water and glycerin, it is preferably in the range of 1:1 to 20:1.

[0016] Also, the weight ratio of the used part of each plant to the extraction solvent is preferably 1:1 to 1:50, and more preferably 1:2 to 1:30.

[0017] When preparing the extract solution, there is no particular limitation on its pH, but generally it is preferably in the range of 3 to 9. In this sense, if necessary, an alkaline regulator such as sodium hydroxide, sodium carbonate, potassium hydroxide, etc., or an acidic regulator such as citric acid, hydrochloric acid, phosphoric acid, sulfuric acid, etc. may be added to the extraction solvent to adjust it to the desired pH.

[0018] The extraction conditions such as the extraction temperature and extraction time vary depending on the type of solvent and pH used. For example, when water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent, the extraction temperature is preferably in the range of 0°C to 80°C, more preferably in the range of 0°C to 20°C, and the extraction time is preferably 1 to 168 hours (1 hour to 1 week), more preferably in the range of 1 to 120 hours (1 hour to 5 days).

[0019] Mix the extract solution prepared as described above, generally adjust the pH to 3 to 8, and then it can be used as it is as a compounding agent for the skin external preparation, or it can be used at a desired concentration by concentration under reduced pressure or the like. Also, the extract may be made into a dried product by a conventional method such as spray drying.

[0020] The solid content concentration of the adlay fermented solution according to the present invention is preferably 0.2% to 5.0% by weight, more preferably 0.3% to 1.5% by weight, in the fermented solution. Further, the solid content concentration of the acteoside extract solution according to the present invention is preferably 0.2 to 5.0% by weight, more preferably 1.0% to 3.0% by weight, in the extract. Further, the solid content concentration of the asparagus racemosus extract solution is preferably 0.2% to 5.0% by weight, more preferably 0.8% to 2.0% by weight, in the extract.

[0021] Mix the adlay fermented solution, acteoside extract solution, and asparagus racemosus extract solution prepared as described above to prepare a composition (hereinafter, the mixed solution is referred to as "composition"). The solid content concentration of the whole composition is preferably 0.8% to 3.0% by weight, more preferably 0.4% to 2.0% by weight, in the composition. Further, the ratio of the solid content concentrations of the adlay fermented solution, acteoside extract solution, and asparagus racemosus extract solution in the composition is preferably 5.5 to 6.0:3.0 to 3.5:1 from the viewpoint of the promoting effect on the expression of the gene of the following moisturizing-related protein.

[0022] When the composition of the present invention is formulated into a skin external preparation, it is preferably formulated at a solid content concentration of 0.01 to 10% by weight or more.

[0023] Examples of the skin external preparation (cosmetics, quasi-drugs, etc.) containing the composition according to the present invention include, for example, emulsions, creams, lotions, essences, transparent gels, sunscreens, packs, lipsticks, foundations, sheet masks, liquid foundations, makeup press powders, blushers, white powders, makeup removers, facial cleansers, body shampoos, hair shampoos, soaps, and other cleansing cosmetics.

[0024] When incorporating the composition according to the present invention into a topical skin preparation (cosmetics, quasi-drugs, etc.), components used in topical skin preparations, such as oily components, surfactants (synthetic or natural), humectants, thickeners, emulsifiers or emulsifying aids, preservatives / bactericides, powder components, UV absorbers, antioxidants, pigments, fragrances, anti-wrinkle agents, and other physiologically active components, may be appropriately incorporated as needed. Furthermore, as long as the effectiveness and characteristics of the composition according to the present invention are not impaired, it may be incorporated into a topical skin preparation in combination with other physiologically active components.

[0025] Here, oily components include, for example, plant-derived oils and fats such as olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, chamomile oil, etc.; vitamin A oil; animal-derived oils and fats such as mink oil, turtle oil, etc.; waxes such as beeswax, carnauba wax, rice wax, lanolin, etc.; liquid paraffin, petrolatum, paraffin wax. Examples include hydrocarbons such as sucrose and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexylglyceride, and higher fatty acid octyldodecyl (octyldodecyl stearate, etc.).

[0026] Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, and polyoxyethylene Anionic surfactants such as ethylene alkylphenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorphonium salts, polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammonium betaine, N,N,N-trialkyl-N-alkyleneammonium carboxybetaine, and N-acylamidopropyl-N′,N′-dimethyl-N′-β-hydroxypropylammonium sulfobetaine can be used.

[0027] As emulsifiers and / or emulsifying aids, stevia derivatives such as enzyme-treated stevia, saponins or their derivatives, casein or its salts (sodium, etc.), sugar-protein complexes, sucrose or its esters, lactose, water-soluble polysaccharides derived from soybeans, complexes of soybean-derived proteins and polysaccharides, lanolin or its derivatives, cholesterol, stevia derivatives (such as enzyme-treated stevia), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their derivatives, lecithin and its derivatives (such as hydrogenated lecithin), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, grains, etc.), etc. may also be included.

[0028] Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid or its salts or derivatives, hydrolyzed hyaluronic acid, chondroitin or its derivatives, heparin or its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, lactic acid, urea, and higher fatty acid octyldodecyl.

[0029] Examples of thickening agents include components derived from brown algae, green algae, or red algae such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose, and hydroxypropylcellulose; synthetic polymers such as carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, polyacrylic acid, etc.

[0030] Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or its derivatives.

[0031] Examples of preservatives and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, alkylisoquinolinium bromide, resorcinol, jamal (imidazodinylurea), isopropylmethylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, hexanediol, concentrated benzalkonium chloride solution 50, peppermint oil, eucalyptus oil, and other essential oils.

[0032] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0033] Examples of anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer 201, and pyridoxine dicaprylate.

[0034] Examples of powder components include sericite, titanium dioxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, anhydrous silicic acid, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powders, grain powders (rice, wheat, corn, millet, etc.), and legume powders (soybeans, adzuki beans, etc.).

[0035] Examples of UV absorbers include ethyl para-aminobenzoate, ethylhexyl para-dimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl para-methoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0036] Examples of antioxidants include carotenoids such as butylhydroxyanisole, butylhydroxytoluene, propyl gallate, and astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), and vitamin A or its derivatives (e.g., retinol palmitate).

[0037] Furthermore, examples of whitening agents include one or more selected from ellagic acid and its derivatives, resorcinol derivatives, potassium 4-methoxysalicylate, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, nicotinic acid derivatives, and AMP (adenosine monophosphate, adenosine monophosphate).

[0038] Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol; examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid; and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. One or more substances selected from kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, potassium 4-methoxysalicylate, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, AMP (adenosine monophosphate, adenosine monophosphate), t-cycloamino acid derivatives, mulberry bark extract, chamomile extract, hydrolyzed rice bran extract, saxifrage extract, and white poppy extract or their hydrolyzed products.

[0039] Examples of kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of ascorbic acid derivatives include ascorbic acid ester salts such as L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, and L-ascorbic acid-2-sulfate magnesium. Ascorbic acid sugar derivatives such as ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl potassium phosphate, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups include hexanoyl, octanoyl, and decanoyl groups), L-ascorbic acid tetra fatty acid esters such as L-ascorbic acid tetraisopalmitate and L-ascorbic acid tetralaurate, 3 As for hydroquinone derivatives, examples include -O-ethyl ascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, L-ascorbic acid aminopropyl phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc. Hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), Examples of tranexamic acid derivatives include lucopyranoside, tranexamic acid esters (e.g., lauryl tranexamic acid ester, hexadecyl tranexamic acid ester, cetyl tranexamic acid ester or their salts), amide forms of tranexamic acid (e.g., methyl tranexamic acid amide), resorcinol derivatives include 4-n-butylresorcinol, 4-isoamylresorcinol, and 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid,Examples of nicotinic acid derivatives include 2-hydroxy-5-propionyloxybenzoic acid, nicotinamide (niacinamide) and benzyl nicotinate, and alpha-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and alpha-hydroxyoctanoic acid.

[0040] Furthermore, examples of anti-wrinkle agents include vitamin A or its derivatives, niacinamide, vitamin E or its derivatives (such as tocopherol acetate), vitamin C or its derivatives (such as ascorbic acid glucoside, 3-O-ethyl ascorbic acid, magnesium ascorbic acid phosphate salt, etc.), pantothenyl alcohol, and tranexamic acid.

[0041] Furthermore, the following natural ingredients derived from plants or microorganisms can also be used in combination: for example, collagen or its hydrolysates, yeast extract or hydrolysates, lactic acid bacteria cultures, grasses, cruciferous plants, camellias, roses, peonyes, citrus plants, amaranthaceae plants, eelgrasses, legumes, daisies, legumes, mallows, gentians, mints, lotus, cucurbits, Araliaceae plants, nightshades, bignoniaceae plants, Actinidiaceae plants, mulberries, irises, bellflowers, olives, Actinidiaceae plants, mulberries, rhamnoides, orchids, and sumac. Examples include extracts or hydrolyzed or fermented products thereof from one or more plants selected from the families of plants, Garcinia, Valenciaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Convolvulaceae, and Asparagaceae; extracts or hydrolyzed or fermented products thereof from one or more seaweeds selected from the families of Laminariaceae, Mylinaceae, and Ulvaceae; jellyfish (autolyzed products of moon jellyfish, Echizen jellyfish, etc.); hydrolyzed or fermented products of hyaluronic acid; and extracts or hydrolyzed or fermented products thereof from royal jelly.

[0042] Next, the present invention will be described in more detail with reference to manufacturing examples, test examples, and formulation examples, but the present invention is not limited thereto. In the following, all parts refer to parts by weight, and all percentages refer to weight percent.

[0043] Production Example 1. Preparation of a fermented adlay solution 50g of hulled Job's tears seeds were crushed, and 950g of purified water was added to prepare a suspension, which was then heat-sterilized. 0.5g of glucoamylase and 0.5g of papain were added to this suspension, and then yeast (Saccharomyces cerevisiae) was added for 10 minutes. 7 The samples were inoculated at 1 / mL and incubated at 37°C for 3 days. After incubation, the cultured samples were heat-sterilized, cooled to room temperature, and filtered to obtain 500g of adlay seed ferment solution (solid content 1.0%).

[0044] Preparation Example 2. Preparation of Salicornia europaea extract solution 20 g of dried, finely chopped whole Salicornia herbacea plants were added to 200 g of purified water and extracted at 40°C for 1 hour. The resulting extract was filtered to obtain 155 g of a clear, brownish extract solution (solids content: 2.00%).

[0045] Preparation Example 3. Preparation of Aspalathus linearis extract solution 100g of the dried whole plant of unfermented rooibos from Aspalathus linearis, a plant of the genus Aspalathus in the legume family, was mixed with 1000g of purified water and 1,3-butylene glycol (7:3) as a solvent. After extraction at 80°C for 2 hours, insoluble matter was removed by filtration to obtain 1025g of dark brown green rooibos extract (solid content concentration 1.50%).

[0046] The fermented product solution of Production Example 1 and the extract solutions of Production Examples 2 and 3, prepared as described above, were mixed and prepared to achieve the optimal solid content concentration ratio, i.e., Job's tears fermented product solution (Production Example 1): Salicornia europaea extract solution (Production Example 2): Aspalathus linearis extract solution (Production Example 3) = 5.5~6.0:3.0~3.5:1. This mixture is referred to as "composition" below, and Test Example 1 and Formulation Example are shown below.

[0047] Test Example 1. Genetic evaluation test of moisturizing-related proteins in the epidermis Normal human epidermal cells (NHEK) were seeded in a 24-well microplate and cultured in HumediaKG2 medium. After 24 hours of incubation, the culture medium was supplemented with the composition according to the present invention as a sample solution. Here, sample solutions were prepared with final concentrations of 1.0% and 2.0% relative to the medium, and these were used. After 24 hours of incubation, the cells were harvested with 500 μL of Trizol reagent (Invitrogen). 100 μL of chloroform was added to the harvested epidermal cells and mixed by stirring. The mixture was then centrifuged at 12,500 rpm at 4°C for 15 minutes, and 200 μL of the aqueous layer was collected. 250 μL of isopropanol was added to the collected aqueous layer and mixed by stirring. The mixture was then centrifuged at 12,500 rpm at 4°C for 10 minutes to obtain a total RNA precipitate. The total RNA was washed by adding 1 mL of 75% cold ethanol and stirring, and the precipitate was collected by centrifugation at 12,500 rpm at 4°C for 10 minutes. The recovered total RNA was reverse transcribed using the specified kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio Inc.)) to synthesize cDNA. The synthesized cDNA was used as a sample in the Thermal Cycler Dice Real Time System Single (Takara Bio Inc.) and SYBR Premix Ex Taq TM Using II (Perfect Real Time) [manufactured by Takara Bio Inc.], we detected the expression of the target gene and the expression of the internal standard GAPDH gene. The test results were calculated by determining the relative expression level of the target gene to the expression level of the GAPDH gene, and then expressed as a relative percentage with the control value set to 100.

[0048] The results of Test Example 1 are shown in Figures 1 to 3. As shown in Figures 1 to 3, it was confirmed that the composition according to the present invention significantly promotes the expression of the filaggrin gene, which is the precursor of natural moisturizing factors (NMF), and the expression of the peptidylarginine deiminase-1 (PADI-1) gene and the peptidylarginine deiminase-3 (PADI-3) gene, which convert arginine residues among the amino acids of filaggrin to citrulline. From this, it can be expected that a topical skin preparation containing the composition according to the present invention will promote the production of amino acids, which are natural moisturizing factors (NMF) in the stratum corneum, thereby maintaining skin moisture and preventing dryness.

[0049] The following are examples of formulations of topical skin preparations containing the composition according to the present invention, such as lotions, emulsions, and creams, but the present invention is not limited to these.

[0050] Prescription example 1. Lotion [Ingredients] Part Jojoba oil 1.0 Composition 3.0 of the present invention Glycerin 5.0 L-ascorbic acid 2-glucoside 2.0 1,3-Butylene glycol 1.0 Water-soluble collagen 0.01 Propanediol 3.0 Potassium hydroxide (appropriate amount) Purified water, in an amount that makes the total volume 100 parts.

[0051] Prescription example 2. Lotion [Ingredients] Part Squalane 1.0 Composition 3.0 of the present invention Glycerin 5.0 Arbutin 3.0 1,3-Butylene glycol 1.0 Hydrolyzed collagen 0.1 Pentanediol 3.0 Sodium citrate (appropriate amount) Purified water, in an amount that makes the total volume 100 parts.

[0052] Prescription example 3. Lotion [Ingredients] Part Hydrogenated castor oil 1.0 Composition 3.0 of the present invention Glycerin 5.0 3-O-ethyl ascorbic acid 2.0 Tocopherol acetate 0.3 Water-soluble collagen 0.01 1,3-Butylene glycol 1.0 Pentanediol 3.0 Potassium hydroxide (appropriate amount) Purified water, in an amount that makes the total volume 100 parts.

[0053] Prescription example 4. Emulsion [Ingredients] Part Squalane 5.0 Jojoba oil 1.0 Orange peel oil 1.0 Polyoxyethylene (20) sorbitan monostearate 1.0 Lipophilic glyceryl stearate 1.0 Hydrogenated soy lecithin 1.5 Composition 1.0 of the present invention Niacinamide 5.0 Glycerin 3.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Sodium hyaluronate 0.01 1,3-Butylene glycol 0.1 Phenoxyethanol 0.5 Pentylene glycol 0.5 Diglycerin 0.3 Purified water, in an amount that makes the total volume 100 parts.

[0054] Prescription example 5. Emulsion [Ingredients] Part Squalane 5.0 Castor oil 1.0 Lipophilic glyceryl stearate 1.0 Hydrogenated soy lecithin 1.5 Composition 1.0 of the present invention Tranexamic acid 2.0 Glycerin 3.0 Carboxymethylcellulose 0.3 Sodium hyaluronate 0.01 Hydrolyzed hyaluronic acid 0.01 1,3-Butylene glycol 0.1 Phenoxyethanol 0.5 Pentylene glycol 0.5 Diglycerin 0.3 Purified water, in an amount that makes the total volume 100 parts.

[0055] Prescription example 6. Cream [Ingredients] Part Olive oil 5.0 Jojoba oil 5.0 Squalane 5.0 Palmitic acid 2.5 Composition 1.0 of the present invention Lactic acid fermented rice 2.0 Hydrogenated lecithin 0.5 Carboxyvinyl polymer 0.3 Sodium alginate 0.2 Water-soluble collagen 0.1 1,3-Butylene glycol 0.1 Phenoxyethanol 0.5 Pentylene glycol 0.5 Diglycerin 0.3 Purified water, in an amount that makes the total volume 100 parts.

[0056] Prescription example 7. Facial cleanser [Ingredients] Part Glyceryl stearate 2.5 Squalane 3.0 Glycerin 3.0 Polyglyceryl-10 laurate 3.0 Composition 3.0 of the present invention Water-soluble collagen 0.01 Sodium hyaluronate 0.01 Isopropylmethylphenol 0.1 1,3-Butylene glycol 2.0 Purified water, in an amount that makes the total volume 100 parts.

Claims

1. A filaggrin gene expression promoter containing a mixture of fermented Job's tears seeds, glasswort extract, and Aspalathus linearis extract as the active ingredient (solid content concentration ratio of fermented Job's tears seeds: glasswort extract: Aspalathus linearis extract = 5.5-6.0:3.0-3.5:1).

2. A topical skin preparation containing a filaggrin gene expression promoter, with the active ingredient being a mixture of fermented Job's tears seeds, glasswort extract, and Aspalathus linearis extract (solid content concentration ratio of fermented Job's tears seeds: glasswort extract: Aspalathus linearis extract = 5.5-6.0:3.0-3.5:1).

3. A topical skin preparation comprising 0.01 to 10% by weight or more of the filaggrin gene expression promoter described in claim 2.

4. A peptidylarginine deiminase-1 (PADI-1) gene expression promoter containing a mixture of fermented Job's tears seeds, glasswort extract, and Aspalathus linearis extract as the active ingredient (solid content concentration ratio of fermented Job's tears seeds: glasswort extract: Aspalathus linearis extract = 5.5-6.0:3.0-3.5:1).

5. A topical skin preparation containing a peptidylarginine deiminase-1 (PADI-1) gene expression promoter, with the active ingredient being a mixture of fermented Job's tears seeds, glasswort extract, and Aspalathus linearis extract (solid content concentration ratio of fermented Job's tears seeds: glasswort extract: Aspalathus linearis extract = 5.5-6.0:3.0-3.5:1).

6. A topical skin preparation comprising 0.01 to 10% by weight or more of the peptidylarginine deiminase-1 (PADI-1) gene expression promoter described in claim 5.

7. A peptidylarginine deiminase-3 (PADI-3) gene expression promoter containing a mixture of fermented Job's tears seeds, glasswort extract, and Aspalathus linearis extract as the active ingredient (solid content concentration ratio of fermented Job's tears seeds: glasswort extract: Aspalathus linearis extract = 5.5-6.0:3.0-3.5:1).

8. A topical skin preparation containing a peptidylarginine deiminase-3 (PADI-3) gene expression promoter, with the active ingredient being a mixture of fermented Job's tears seeds, glasswort extract, and Aspalathus linearis extract (solid content concentration ratio of fermented Job's tears seeds: glasswort extract: Aspalathus linearis extract = 5.5-6.0:3.0-3.5:1).

9. A topical skin preparation comprising 0.01 to 10% by weight or more of the peptidylarginine deiminase-3 (PADI-3) gene expression promoter described in claim 8.

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