Apple fermentation composition

Abstract

The present application relates to a fermented apple composition having the effect of reducing or suppressing the expression of collagen decomposition enzymes caused by ultraviolet rays, and uses thereof for improving skin and preventing or suppressing skin photoaging.

Description

Technical Field

[0001] This application relates to an apple fermentation composition and its use for improving the skin, preventing or suppressing skin photoaging.

Background Art

[0002] Recently, regarding skin beauty, there has been an increasing interest in delaying or alleviating skin aging. In particular, along with the increasing interest in photoaging caused by ultraviolet exposure in addition to natural aging, a large number of product developments have been carried out to prevent or suppress it.

[0003] Photoaging is a phenomenon in which collagen in the dermis is decomposed by reactive oxygen species (ROS) generated in skin cells by ultraviolet rays. When ROS generated by ultraviolet stimulation penetrates into cells and induces the expression of MMP-1 (Matrix metalloproteinase-1), which is a collagen-degrading enzyme, the overexpressed MMP-1 decomposes the collagen in the skin dermis, causing the skin to lose elasticity and age. Therefore, in order to prevent or suppress photoaging, it is necessary to develop substances that can effectively suppress MMP-1 expression.

[0004] On the other hand, an apple is the fruit of an apple tree, which is a perennial plant belonging to the genus Malus of the family Rosaceae, and is rich in sugar, vitamin A, vitamin C, and inorganic salts. Vitamin C, which is contained in a large amount in apples, has an excellent effect of regenerating damaged skin cells by firmly binding the keratin and collagen of the skin. Furthermore, apple extracts are known to have a soothing and moisturizing effect on the skin, and apples and apple juice contain malic acid, tartaric acid, and the enzyme amylase, and are known to have an effect of removing skin keratin.

[0005] Currently, various food and cosmetic compositions using apples are being developed. However, as domestic and international demand for compositions that can prevent or suppress photoaging and improve skin condition increases, there is a need to develop new compositions with superior photoaging prevention or suppression effects. [Overview of the project] [Problems that the invention aims to solve]

[0006] This application provides an apple fermentation composition and its use for skin improvement, prevention or inhibition of skin photoaging. [Means for solving the problem]

[0007] One object of this application is to provide an apple fermentation composition that has the effect of reducing or suppressing the expression of collagen-degrading enzymes caused by ultraviolet light. Another object of this application is to provide a food product comprising the apple fermentation composition of this application.

[0008] Another object of this application is to provide a health functional food containing the apple fermentation composition of this application. Another object of this application is to provide a cosmetic composition comprising the apple fermentation composition of this application. [Effects of the Invention]

[0009] The apple fermentation composition of this application has the effect of reducing or suppressing the expression of collagen-degrading enzymes caused by ultraviolet light, and therefore can be used for skin improvement, prevention or suppression of skin photoaging. [Brief explanation of the drawing]

[0010] [Figure 1] This shows the results of examining MMP-1 (Matrix metalloproteinase-1) expression levels after treating HaCaT cells irradiated with UVB with apple fermentation compositions or apple concentrate at different concentrations. * indicates p<0.05, ** indicates p<0.01. [Modes for carrying out the invention]

[0011] This can be explained in more detail as follows. On the other hand, each description and embodiment disclosed in this application can be applied to each other description and embodiment. In other words, all combinations of the various elements disclosed in this application belong to the category of this application. Furthermore, the category of this application cannot be seen as being limited by the specific descriptions described below. In addition, numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated into this specification by reference as a whole, and the level of the technical field to which this application belongs and the content of this application are explained more clearly.

[0012] One aspect of this application provides an apple fermentation composition that has the effect of reducing or suppressing the expression of collagen-degrading enzymes caused by ultraviolet light. The apple fermentation composition of this application may also contain purified water and apple concentrate.

[0013] In this application, the term "apple concentrate" means a liquid obtained by concentrating apples, and may be used without limitation as to be manufactured or commercially available. For example, the apple concentrate may be 100% apple concentrate, but is not limited thereto. Furthermore, the apple concentrate may have a sugar content of 60 Brix or higher, the standard sugar content of a refractometer, but is not limited thereto. In addition, the apple concentrate may have an acidity of 6% or less, but is not limited thereto.

[0014] In one example, in the apple fermentation composition of this application, the apple concentrate may mean the components contained before fermentation. Specifically, in the apple fermentation composition of this application, the apple concentrate may be contained in amounts of 1-50% (v / v), 1-30% (v / v), 1-25% (v / v), 5-50% (v / v), 5-30% (v / v), 5-25% (v / v), 10-50% (v / v), 10-30% (v / v), 10-25% (v / v), 15-50% (v / v), 15-30% (v / v), or 15-25% (v / v) relative to the total volume of the apple fermentation composition.

[0015] In this application, the term "apple fermentation composition" means a fermented product obtained by fermenting apple concentrate. In this application, the term "fermentation" usually refers to the phenomenon or process by which microorganisms use their enzymes to decompose organic matter, converting and accumulating metabolites.

[0016] In this application, the term "fermented product" may be interpreted comprehensively to include, but is not limited to, all substances produced by the fermentation of this application, their filtrate, their dilution, their concentrate, their crude product, or their refined product.

[0017] Specifically, the fermented product of this application may be an acetic acid fermented product or vinegar. More specifically, the fermented product of this application may be a yeast and acetic acid bacteria fermented product. Even more specifically, the fermented product of this application may be a fermented product obtained by yeast fermentation followed by acetic acid bacteria fermentation.

[0018] For example, the apple fermentation composition of this application may be an apple acetic acid fermentation composition, apple acetic acid ferment, or apple vinegar obtained by fermenting apples with acetic acid bacteria. Alternatively, the apple fermentation composition of this application may be an apple yeast fermentation and acetic acid bacteria fermentation composition obtained by fermenting apples with yeast and acetic acid bacteria. Furthermore, the apple fermentation composition of this application may be an apple yeast fermentation followed by acetic acid bacteria fermentation composition obtained by fermenting apples with yeast and then acetic acid bacteria.

[0019] In the present application, the apple fermentation composition of the present application may have an effect of suppressing collagen degradation by ultraviolet rays. As an example, the suppression of collagen degradation can be achieved by a decrease or suppression of the expression of collagen-degrading enzymes.

[0020] The collagen-degrading enzyme of the present application may be MMP-1 (Matrix metalloproteinase-1), MMP-2 or MMP-9, and any collagen-degrading enzyme known in the art may be included within the scope of the present application. As an embodiment, the collagen-degrading enzyme may be MMP-1.

[0021] When the apple fermentation composition of the present application is treated on cells irradiated with ultraviolet rays, it can decrease or suppress the expression of collagen-degrading enzymes by ultraviolet rays as compared with ultraviolet-irradiated cells not treated with the apple fermentation composition.

[0022] As an example, the apple fermentation composition can decrease or suppress the expression of collagen-degrading enzymes by ultraviolet rays within a range composed of one lower limit selected from 10% or more, 15% or more, 20% or more, 21% or more, 22% or more, 23% or more, 24% or more, 25% or more, 26% or more, 27% or more, 28% or more, 29% or more, and 29.1% or more and / or one upper limit selected from 100% or less, 50% or less, 40% or less, and 35% or less, but is not limited thereto.

[0023] In the present application, the apple fermentation composition of the present application may have a skin-improving effect. In the present application, the apple fermentation composition of the present application may have an effect of preventing skin aging.

[0024] In the present application, the term "skin improvement" means an improvement in the skin condition due to the effect of preventing skin aging. Alternatively, it may mean an improvement or enhancement of skin health. The aging may be photoaging. More specifically, the aging may be skin aging caused by ultraviolet rays.

[0025] The aforementioned improvement of skin or prevention of skin aging can be achieved, for example, by inhibiting collagen degradation and / or reducing or suppressing the expression of collagen-degrading enzymes, as described above.

[0026] In this application, the apple fermentation composition may also have the effect of suppressing wrinkle formation caused by ultraviolet light. The aforementioned suppression of wrinkle formation can be achieved, for example, by inhibiting collagen degradation and / or reducing or suppressing the expression of collagen-degrading enzymes, as described above.

[0027] Another aspect of this application is to provide a food product comprising the apple fermentation composition of this application. The aforementioned apple fermentation composition is as described above. In this application, the food of this application may have an effect of improving or enhancing skin health. The improvement or enhancement of skin health can be achieved by inhibiting collagen degradation and / or reducing or suppressing the expression of collagen-degrading enzymes, as previously described in the "skin improvement" section.

[0028] Another aspect of this application is to provide a health functional food containing the apple fermentation composition of this application. The aforementioned apple fermentation composition is as described above.

[0029] In this application, the health functional food of this application may have an effect of improving or enhancing skin health. The improvement or enhancement of skin health can be achieved by inhibiting collagen degradation and / or reducing or suppressing the expression of collagen-degrading enzymes, as previously described in the "skin improvement" aspect.

[0030] The term "food" in this application may include any form such as general foods, functional foods, nutritional supplements, health foods, and food additives.

[0031] The aforementioned type of food can be manufactured in various forms according to common methods known to the industry. The aforementioned food products include, but are not limited to, various foods to which the composition of this application can be added, such as, rice, edible grain flour, grain soup, rice bowls, noodles, gukbap, instant rice, seasonings, bento boxes, dried cooked rice, bread, edible sugars, rice cakes, bibimjang, sauces, spices, edible salt, seasonings, seasoning powders, processed, frozen, dried and cooked fruits and vegetables, jelly, jam, candied fruit, eggs, milk and other dairy products, edible oils and fats, coffee, cocoa and coffee substitutes, tapioca, grain flour and grain preparations, ramen, udon, noodles, kalguksu, naengmyeon, porridge, soup, soup dishes, instant foods, frozen foods, ready-to-eat foods, retort foods, other beverages, gum, tea, vitamin complexes, and health supplements.

[0032] The food product of this application may contain various herbal extracts, food additives, or natural carbohydrates as additional ingredients, similar to those found in ordinary foods. Furthermore, the food additives may include those commonly used in the industry, such as flavoring agents, flavor enhancers, colorants, fillers, and stabilizers.

[0033] Examples of the aforementioned natural carbohydrates include monosaccharides, such as glucose and fructose; disaccharides, such as maltose and sucrose; and polysaccharides, such as common sugars like dextrin and cyclodextrin; and sugar alcohols such as xylitol, sorbitol, and erythritol. In addition to the foregoing, natural flavorings (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavorings (e.g., saccharin, aspartame, etc.) may be used as flavoring agents. Furthermore, common food additives used to enhance taste and nutrition, such as nucleic acids, amino acids, and organic acids, may also be added.

[0034] In addition to the foregoing, the food of this application may contain various nutrients, vitamins, minerals (electrolytes), flavorings such as synthetic and natural flavorings, colorings and fillers (for cheese, chocolate, etc.), pectin and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, and carbonating agents used in carbonated beverages. It may also contain fruit pulp for the production of natural fruit juices, fruit juice beverages, and vegetable beverages. These components may be used independently or in combination.

[0035] The food product of this application can be manufactured by methods commonly used in the industry, and in such manufacturing, raw materials and ingredients commonly added in the industry may be added. Furthermore, the dosage form of the food product can be any dosage form recognized as a food product, without any limitations.

[0036] Furthermore, when the food product of this application is used as a health functional food, it can be manufactured in various dosage forms, and unlike general medicines, it has the advantage of not having side effects that may occur with long-term use of medicines, as it is made from food as a raw material, and it is highly portable and can be taken as an adjunct.

[0037] On the other hand, the food products of this application may also include flavoring agents, tasters, colorants, fillers, stabilizers, and seasoning materials, which can also be classified as food additives. The term "flavor" in this application may refer to a material added to food to enhance its flavor. The flavor material may also refer to a material that causes the food to have excellent milling properties.

[0038] The aforementioned seasoning ingredients can be classified according to their flavor components. That is, they can be classified according to their flavor into neutral seasoning ingredients, beef-flavored seasoning ingredients, chicken-flavored seasoning ingredients, pork-flavored seasoning ingredients, and kokumi (savory) seasoning ingredients.

[0039] "Kokumi flavor" refers to a seasoning ingredient that produces a rich, savory taste. The term "kokumi" itself is derived from Japanese and is expressed in English as "mouthfulness," "continuity," "thickness," or "heartiness." In Korean, it can be expressed as "rich flavor," "full flavor," "flavor that fills the mouth," "intense flavor," or "true flavor." "Neutral flavor" refers to a seasoning ingredient that maximizes umami and minimizes other flavors to produce a pure and clean taste. For example, oils such as canola oil and grape seed oil can be described as having a neutral flavor. "Rice polishing properties" can mean having sourness, sweetness, saltiness, bitterness, umami, etc., but is not limited to these.

[0040] The food product of this application may be an apple cider vinegar or fruit fermented drinking vinegar product of CJ CheilJedang Co., Ltd., for example, a Petit Chel MiCho (trademark) or MiCho (registered trademark) product, but is not limited thereto. The food product of this application may contain the apple fermentation composition of this application at a concentration of 1-50% (v / v), 1-30% (v / v), 5-50% (v / v), 5-30% (v / v), or 10-30% (v / v) relative to the total volume of the food product, but is not limited thereto.

[0041] Another aspect of this application provides a cosmetic composition comprising the apple fermentation composition of this application. The aforementioned apple fermentation composition is as described above.

[0042] In this application, the cosmetic composition of this application may have an effect of reducing or improving wrinkles in the skin. The reduction or improvement of wrinkles can be achieved by inhibiting collagen degradation and / or reducing or suppressing the expression of collagen-degrading enzymes, as previously described in the section on "inhibition of wrinkle formation".

[0043] In this application, the cosmetic composition of this application may have a skin-improving or anti-aging effect. The skin-improving or anti-aging effect can be achieved by inhibiting collagen degradation and / or reducing or suppressing the expression of collagen-degrading enzymes, as previously described.

[0044] The "cosmetic composition" of this application can be manufactured in dosage forms selected from the group consisting of solutions, topical ointments, creams, foams, nourishing lotions, softening lotions, packs, softening waters, emulsions, makeup bases, essences, soaps, liquid cleansers, bath additives, sunscreen creams, sun oils, suspensions, emulsions, pastes, gels, lotions, powders, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, patches, and sprays, but is not limited thereto.

[0045] The cosmetic composition may further contain one or more cosmetically acceptable carriers used in general skin cosmetics, and may, but is not limited to, oils, water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc., as usual ingredients. A person skilled in the art can appropriately select the cosmetically acceptable carriers included in the cosmetic composition of this application depending on the dosage form of the cosmetic composition.

[0046] The cosmetic composition of this application may contain the apple fermentation composition of this application at a concentration of 0.001-2% (v / v), 0.005-1.9% (v / v), 0.02-1.8% (v / v), 0.025-1.75% (v / v), 0.03-1.7% (v / v), 0.035-1.65% (v / v), 0.04-1.6% (v / v), 0.045-1.55% (v / v), or 0.05-1.5% (v / v) relative to the total volume of the cosmetic composition. As an example, the cosmetic composition may contain the apple fermentation composition of this application at a concentration of 0.05-1.5% (v / v) relative to the total volume of the cosmetic composition, but is not limited thereto.

[0047] Another aspect of this application provides a method for reducing or suppressing the expression of UV-induced collagen-degrading enzymes, comprising the step of administering the apple fermentation composition of this application. The collagen-degrading enzyme may be MMP-1, but is not limited to it. This is as previously stated.

[0048] The aforementioned apple fermentation composition may, but is not limited to, reducing or suppressing the expression of UV-induced collagen-degrading enzymes by 10% or more when applied to UV-irradiated cells compared to UV-irradiated cells that were not treated with the apple fermentation composition. This is as described above.

[0049] Another aspect of this application provides a method for improving skin, comprising the step of administering the apple fermentation composition of this application. Another aspect of this application provides a method for preventing skin aging, comprising the step of administering the apple fermentation composition of this application.

[0050] The aforementioned aging may be, but is not limited to, photoaging. This is as previously stated. Another aspect of this application provides a method for inhibiting wrinkle formation by ultraviolet light, which includes the step of administering the apple fermentation composition of this application.

[0051] In this application, the term "administration" means introducing the composition of this application into an individual in which inflammatory cytokines are overexpressed or who is suspected of having enteritis, by any appropriate means. The pharmaceutical compositions of this application are not particularly limited and can be applied to any individual whose purpose is to reduce or suppress the expression of collagen-degrading enzymes caused by ultraviolet light, or to improve skin, prevent skin aging, or suppress wrinkle formation caused by ultraviolet light. For example, they can be used in non-human animals such as monkeys, dogs, cats, rabbits, guinea pigs, rats, mice, cattle, sheep, pigs, and goats, as well as birds and fish.

[0052] The composition of this application may be administered in an amount effective for reducing or suppressing the expression of UV-induced collagen-degrading enzymes, in an amount effective for improving skin, in an amount effective for preventing skin aging, or in an amount effective for suppressing wrinkle formation caused by UV light. The preferred dosage of the composition of this application will vary depending on the individual's condition and weight, the severity of the disease, the drug form, the route of administration, and the duration, but can be appropriately selected by those skilled in the art.

[0053] Furthermore, the compositions of this application can be administered via various routes, orally or parenterally, as long as they can reach the target tissue. For example, they can be administered orally, parenterally, subcutaneously, intraperitoneally, intrapulmonaryly, intranasally, rectally, or intravenously, intramuscularly, intrauterine dura materally, or intravascularly, and for local treatment, they can be administered by appropriate methods, including intra-lesional administration as needed. However, they are not limited thereto.

[0054] In one example, the apple fermentation composition may be administered in the form of a food composition containing it. In this case, the administration may be done orally. In another embodiment, the apple fermentation composition may be administered in the form of a health functional food composition containing it. In this case, the administration may be by oral administration.

[0055] Another aspect of this application provides a method for reducing or suppressing the expression of UV-induced collagen-degrading enzymes, comprising the step of applying the apple fermentation composition of this application to the skin. Another aspect of this application provides a skin improvement method comprising the step of applying the apple fermentation composition of this application to the skin.

[0056] Another aspect of this application provides a method for preventing skin aging, which includes the step of applying the apple fermentation composition of this application to the skin. Another aspect of this application provides a method for suppressing wrinkle formation caused by ultraviolet light, which includes the step of applying the apple fermentation composition of this application to the skin.

[0057] In this application, the term “application” means bringing the compositions of the present invention into contact with the skin of an individual in any suitable manner, including, but not limited to, any action aimed at causing the compositions to be absorbed into the skin.

[0058] As an example, the apple fermentation composition may be applied to the skin in the form of a cosmetic composition containing it. Another aspect of this application provides an application for reducing or suppressing the expression of UV-induced collagen-degrading enzymes in the apple fermentation composition of this application.

[0059] The collagen-degrading enzyme may be MMP-1, but is not limited to it. This is as previously stated. The aforementioned apple fermentation composition may, but is not limited to, reducing or suppressing the expression of UV-induced collagen-degrading enzymes by 10% or more when applied to UV-irradiated cells compared to UV-irradiated cells that were not treated with the apple fermentation composition. This is as described above.

[0060] Another aspect of this application is to provide the apple fermentation composition of this application for skin improvement applications. Another aspect of this application is to provide the apple fermentation composition of this application for use in preventing skin aging.

[0061] The aforementioned aging may be, but is not limited to, photoaging. This is as previously stated. Another aspect of this application is to provide an application for suppressing wrinkle formation caused by ultraviolet light in the apple fermentation composition of this application.

[0062] Another aspect of this application provides a food product containing the apple fermentation composition of this application for use in improving or enhancing skin health. Another aspect of this application provides a health functional food containing the apple fermentation composition of this application for use in improving or promoting skin health.

[0063] Another aspect of this application provides a cosmetic composition comprising the apple fermentation composition of this application for use in reducing or improving skin wrinkles. The terminology used here is as described above in the previous section. [Examples]

[0064] The present application will be described in more detail below with reference to examples. However, the following examples are merely preferred embodiments for illustrative purposes and are not intended to limit the scope of the rights of this application. On the other hand, technical matters not described herein can be easily understood and implemented by an ordinary person skilled in the art of this application or a similar art.

[0065] Manufacturing Example 1: Production of an apple fermentation composition The apple concentrate used was a commercially available product made from 100% concentrated apples. It was prepared by diluting a Chinese apple concentrate with a standard sugar content of 60 Brix or higher and an acidity of 6% or less with distilled water at a ratio of 4:1 (w / w) to approximately 20 Brix.

[0066] The apple fermentation composition used was commercially available apple cider vinegar (containing 20.86% yeast and acetic acid bacteria fermented product, purified water, and apple concentrate; acidity 6-8%; CJ Cheil Sugar Co., Ltd. Healthy Fermented Apple Cider Vinegar). To remove microorganisms from the apple fermentation composition and apple concentrate, they were filtered using a 0.2 μm filter.

[0067] Example 1: Evaluation of photoaging inhibition ability in vitro The photoaging inhibitory ability of apple fermentation composition and apple concentrate was analyzed using HaCaT cells in which overexpression of the collagen-degrading enzyme MMP-1 (Matrix metalloproteinases-1) was induced by UVB irradiation.

[0068] Specifically, HaCaT cell lines (Korea Cell Bank) were cultured in FBS-contained DMEM medium (Gibco, USA) at 37°C and 5% CO2, and cells between 8 and 12 passages were obtained. The obtained HaCaT cells were cultured in FBS-contained DMEM medium in a 0.7 × 10⁶ solution. 5 The cells were diluted to a cell / well ratio and seeded into a 24-well plate, then cultured at 37°C and 5% CO2 for 2 days. To induce starvation conditions, the medium was changed with FBS-free DMEM and cultured for 1 day (Figure 1, shown as NC). Subsequently, the cells were washed twice with PBS (Phosphate-Buffered Saline, Gibco), and 500 ml of PBS was added to each well, followed by UVB 20 mJ / cm². 2 MMP-1 overexpression was induced by irradiation, and the cells were further washed twice with PBS (Figure 1, shown as CON). As an experimental group, the apple fermentation composition and apple concentrate from Production Example 1 were each diluted to a 1% (v / v) concentration in FBS-free DMEM and treated with the cells (CON), followed by incubation for 1 day. After incubation, the supernatant was taken and centrifuged at 4°C and 13,000 rpm for 3 minutes to obtain the supernatant from which the cells had been removed. Subsequently, the supernatant was filtered using a 0.2 μm filter to remove any remaining bacterial cells.

[0069] The obtained supernatant was analyzed using an ELISA assay to measure the concentration of MMP-1. Specifically, the ELISA assay was performed using Human Total MMP-1 DuoSet ELISA (R&D systems) according to the manufacturer's instructions as follows: First, capture antibody was deposited overnight into each well of a 96-well plate. After washing three times with washing buffer, the wells were treated with reagent diluent for 1 hour. Subsequently, after three further washes with washing buffer, the supernatant and standard solution were added to each well separately for each experimental group and control group (NC, CON), and incubated for 2 hours. After washing, detection antibody was added and incubated for 2 hours. After further washing, each well was treated with streptavidin-HRP B for 20 minutes. After stopping the reaction with stop solution, the absorbance was measured at 450 nm, and the MMP-1 concentration was calculated using the 4-parameter logistic assay.

[0070] As a result, both the fermented apple composition and the apple concentrate significantly reduced MMP-1 at a concentration of 1% (v / v), with the fermented apple composition (29.1%) showing a greater reduction in MMP-1 compared to the apple concentrate (11.5%) (Figure 1 and Table 1).

[0071] This study confirmed that both the fermented apple composition and the concentrated apple liquid exhibited photoaging inhibitory properties, and that the fermented apple composition, in particular, demonstrated superior photoaging inhibitory properties compared to the concentrated apple liquid.

[0072] [Table 1]

[0073] From the above description, a person skilled in the art will understand that this application can be implemented in other specific forms without altering its technical idea or essential features. In this regard, it should be understood that the embodiments described above are illustrative and not restrictive in all respects. The scope of this application should be interpreted as encompassing all modified or altered forms derived from the meaning and scope of the claims and their equivalent concepts, as described in the above detailed description. (Note) The technical concepts that can be understood from the above embodiments and modified examples are described below. [Item 1] An apple fermentation composition that has the effect of reducing or suppressing the expression of collagen-degrading enzymes caused by ultraviolet light. [Item 2] The apple fermentation composition according to item 1, wherein the collagen-degrading enzyme is MMP-1. [Item 3] The apple fermentation composition according to item 1, wherein the apple fermentation composition is an apple cider vinegar fermentation composition. [Item 4] The apple fermentation composition according to item 3, wherein the apple fermentation composition is an apple yeast fermentation and acetic acid bacteria fermentation composition. [Item 5] The apple fermentation composition according to item 1, wherein the apple fermentation composition has a skin-improving effect. [Item 6] The apple fermentation composition described in item 1, wherein the apple fermentation composition has an anti-aging effect on the skin. [Item 7] The apple fermentation composition according to item 6, wherein the aging is photoaging. [Item 8] The apple fermentation composition according to item 1, wherein the apple fermentation composition has the effect of suppressing collagen degradation caused by ultraviolet light. [Item 9] The apple fermentation composition according to item 1, wherein when the apple fermentation composition is applied to cells irradiated with ultraviolet light, it reduces or suppresses the expression of UV-induced collagen-degrading enzymes by 10% or more compared to UV-irradiated cells that were not treated with the apple fermentation composition. [Item 10] The apple fermentation composition according to item 1, wherein the apple fermentation composition has the effect of suppressing wrinkle formation caused by ultraviolet light. [Item 11] A food product containing the apple fermentation composition described in item 1. [Item 12] The food described in item 12 is for the purpose of improving or promoting skin health. [Item 13] A health functional food containing the apple fermentation composition described in item 1. [Item 14] The health functional food described in item 13 is for the improvement or promotion of skin health. [Item 15] A cosmetic composition comprising the apple fermentation composition described in item 1. [Item 16] The cosmetic composition according to item 15, wherein the cosmetic composition is for the purpose of reducing or improving wrinkles on the skin.

Claims

1. Use of a food composition comprising an apple fermentation composition for reducing or suppressing the expression of the collagen-degrading enzyme MMP-1 caused by ultraviolet light, The aforementioned apple fermentation composition includes a fermented product of apple concentrate and purified water by yeast and acetic acid bacteria. Use (excluding medical procedures involving humans).

2. The use according to claim 1, wherein the apple fermentation composition has the effect of suppressing collagen degradation caused by ultraviolet light.

3. The use according to claim 1, wherein when the apple fermentation composition is applied to cells irradiated with ultraviolet light, the expression of collagen-degrading enzymes induced by ultraviolet light is reduced or suppressed by 10% or more compared to ultraviolet-irradiated cells that were not treated with the apple fermentation composition.

4. Use of a health functional food comprising an apple fermentation composition for alleviating or improving skin wrinkles by reducing or suppressing the expression of the collagen-degrading enzyme MMP-1 caused by ultraviolet light, The aforementioned apple fermentation composition includes a fermented product of apple concentrate and purified water by yeast and acetic acid bacteria. Use (excluding medical procedures involving humans).

5. A method for alleviating or improving skin wrinkles by reducing or suppressing the expression of the collagen-degrading enzyme MMP-1 caused by ultraviolet light, The procedure includes administering an apple fermentation composition. The aforementioned apple fermentation composition includes a fermented product of apple concentrate and purified water by yeast and acetic acid bacteria. Methods (excluding medical procedures performed on humans).

6. A method for reducing or suppressing the expression of the ultraviolet-induced collagen-degrading enzyme MMP-1, The procedure includes administering an apple fermentation composition. The aforementioned apple fermentation composition includes a fermented product of apple concentrate and purified water by yeast and acetic acid bacteria. Methods (excluding medical procedures performed on humans).

7. A food composition for alleviating or improving skin wrinkles by reducing or suppressing the expression of the collagen-degrading enzyme MMP-1 caused by ultraviolet light, Contains an apple fermentation composition, The aforementioned apple fermentation composition includes a fermented product of apple concentrate and purified water by yeast and acetic acid bacteria. Food composition.

8. The food composition according to claim 7, wherein the apple fermentation composition has the effect of suppressing collagen degradation caused by ultraviolet light.

9. The food composition according to claim 7, wherein when the apple fermentation composition is applied to cells irradiated with ultraviolet light, the expression of collagen-degrading enzymes due to ultraviolet light is reduced or suppressed by 10% or more compared to ultraviolet-irradiated cells that were not treated with the apple fermentation composition.

10. A health functional food for alleviating or improving skin wrinkles by reducing or suppressing the expression of the collagen-degrading enzyme MMP-1 caused by ultraviolet light, Contains an apple fermentation composition, The aforementioned apple fermentation composition includes a fermented product of apple concentrate and purified water by yeast and acetic acid bacteria. Healthy functional food.

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