Solution for regenerating passivation of dental implants and reducing bacterial activity, and uses of said solution

Abstract

The present invention relates to a solution for regenerating passivation of dental implants and reducing bacterial activity, the composition of which contains between 10 vol-% and 50 vol-% citric acid solution and, per litre of solution, between 0.2 g and 1.0 g collagen and between 5 g and 50 g salts of divalent magnesium or calcium ions. The invention comprises the use of the solution as a mouthwash, an agent for improving hydrophilicity and osseointegration, and an agent for reducing bacterial activity in dental implants.

Description

[0001]DESCRIPTIONSolution for the regeneration and passivation of dental implants and the reduction of bacterial activity, and its uses.Technical sector.The present invention relates to a solution for the regeneration and passivation of dental implants, including abutments made of materials with osteogenic capacity, and the reduction of bacterial activity. Said solution is applicable to dental health and is applied to dental implants and accessories such as crowns and abutments, used in dental implantology.Prior state of the art Today, the placement of dental implants is the most widely used treatment for replacing patients' teeth. These dental implants have allowed patients to restore aesthetics, chewing ability, and phonetic functions. Although the success rate of this treatment is high, in some cases complications may arise due to the adhesion of bacterial colonies or poor osseointegration.To avoid the failure of dental implants, the physical-chemical properties and topography of the surface must be taken into account, since they are of great importance in increasing the rate of osseointegration and decreasing bacterial colonization on the surface. Therefore, the technical problem that arises is to increase the osseointegration and antibacterial properties of implants, abutments and crowns in order to increase the success rate of implant placement. Explanation of the invention. To solve the technical problem posed, a liquid solution has been developed, the objective of which is to produce a coating with antibacterial and osseointegrative properties on dental implants, abutments and crowns, by immersing them for a certain period of time in said solution.According to the invention, said solution comprises: collagen, a solution of citric acid and salts of divalent ions such as magnesium or calcium; in the following proportions: for each liter of citric acid solution of 10 to 50% by volume, between 0.2 and 1.0 grams of collagen and between 5 and 50 grams of salts of divalent ions of magnesium or calcium. The ability to increase osseointegration and antibacterial properties, in addition to increasing the success rate of implant placement, constitutes a solution for machined surfaces after implantoplasty. For the development of the invention, studies were carried out consisting of determining the immersion time of the implant / crown in the solution based on the collagen concentration and the determination of the contact angle on the surface. Immersion times of less than 10 minutes were worked with, taking into account the clinical application of the solution.The cellular response (cytotoxicity control) of the samples was studied with the optimal immersion time determined above, observing cell adhesion with osteoblastic cells (SaOS-2). Finally, the antibacterial properties of the samples were analyzed at the optimal time by monitoring the growth of the bacterial strain Streptococcus gordonii. This invention includes the use of the aforementioned solution as: a mouthwash for dental health applied to dental implants and their accessories; an agent to improve the hydrophilicity of the surface of dental implants; an agent to improve the osseointegration of dental implants; and an agent to reduce bacterial activity on the surface of dental implants. Brief description of the content of the drawings.In order to complement the description being provided, this specification is accompanied by a set of drawings which, for illustrative but not limiting purposes, include graphs showing the results of experimental methods carried out using the solution of the invention, specifically: - Figure 1 shows a graph showing the results obtained after measurements of the contact angle of the surfaces of discs made of different materials, carried out on control samples (Ctrl) and on samples treated for 2 and 10 minutes with the solution of the invention. - Figure 2 shows a graph showing the cytocompatibility results for different materials and different treatments. - Figure 1 shows a graph showing the results obtained from the bacterial adhesion test using the “Alamar Blue” test. Detailed description of embodiments of the invention.The materials and experimental methodology used are listed below. Titanium (grade 4), Zirconium, Composite, and Cobalt-Chrome discs were immersed in four different solutions. After immersion for 2 and 10 minutes, the samples were analyzed by scanning electron microscopy (SEM) and the contact angle (CA) was evaluated to determine the optimal treatment time and surface wettability. The studied solutions are shown in the following table: Osteoblastic lineage cells (SaOS-2) were used to determine cell viability and proliferation capacity. Cell attachment and cytotoxicity were determined by the Resazurin Sodium Salt (Alamar Blue) assay and cell morphology by the confocal assay (F-Actin (Phalloidin) Immunofluorescence) after 3 days of incubation. For the evaluation of bacterial activity, the bacterial strain S. Gordonii was used. The antibacterial properties of the proposed treatments were determined by the Resazurin Sodium Salt (Alamar Blue) assay after 1 day of incubation. As shown in the graph in Figure 1, treatments of the different materials (Titanium, Zirconium, Composite and Cobalt-Chromium) with the solution of the invention have considerably decreased the contact angle of the treated samples with respect to the control samples.The treatments have provided the sample surfaces with a hydrophilic / superhydrophilic character. The combination of the constituent elements of the solution of the invention and used in this study has been able to provide cell viability greater than 70%, which is when no cytotoxicity is considered present, therefore, promoting the adhesion and proliferation of osteoblasts. In addition, it also provides the surface with an antibacterial character without damaging the cells. Figure 2 shows the results of the cellular cytotoxicity test for the surface of the four proposed materials under the five conditions. In all cases, it has been observed that they exceed 70% cell viability and therefore the treatment with the solution of the invention is cytocompatible. Figure 3 shows the results obtained after the Alamar Blue test to determine bacterial adhesion and growth on the surfaces proposed for the study.Titanium, Zirconium and Composite surfaces respond favorably to the four applied treatments, reducing the adhesion of bacteria on the samples. On the other hand, on Chromium - Cobalt surfaces none of the applied treatments favor the reduction of bacteria in the samples. However, the treatment that stands out for its results over the other treatments is the one carried out with the solution of the invention containing 25% Citric Acid + 500 Collagen + 1% Mg. Once the nature of the invention has been sufficiently described, it is stated for the appropriate purposes that the same may include any modifications that are considered appropriate, provided that this does not entail an alteration of the essential characteristics of the invention claimed below.

Claims

CLAIMS1. Solution for regeneration of passivated dental implants and reduction of bacterial activity, characterized in that its composition comprises a citric acid solution of 10 to 50% by volume and, for each liter of solution, between 0.2 and 1.0 grams of collagen and between 5 and 50 grams of divalent ion salts of magnesium or calcium.

2. The use of the solution of claim 1 as a mouthwash for dental health applied to dental implants and their accessories.

3. The use of the solution of claim 1 as an agent to improve the hydrophilicity of the surface of dental implants.

4. The use of the solution of claim 1 as an agent to improve the osseointegration of dental implants.

5. The use of the solution of claim 1 as an agent to reduce bacterial activity on the surface of dental implants.

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