Emulsifier system

Abstract

The disclosure relates to an emulsifier system including a formulation including a polysaccharide component and a silk fibroin component, the silk fibroin component including a plurality of fibroin heavy chain peptides or protein fragments, a plurality of fibroin light chain peptides or protein fragments, and / or a plurality of fibrohexamerin (p25) plurality of peptides or protein fragments.

Description

EMULSIFIER SYSTEMCROSS REFERENCE TO RELATED APPLICATIONSThe present application claims priority to and the benefit of U.S. Provisional Patent Application No. 63 / 567,924, filed on March 20, 2024, U.S. Provisional Patent Application No. 63 / 683,051. filed on August 14, 2024, U.S. Provisional Patent Application No. 63 / 691,540, filed on September 6, 2024, U.S. Provisional Patent Application No. 63 / 694,126, filed on September 12, 2024, and U.S. Provisional Patent Application No. 63 / 720,582, filed on November 14, 2024, each of which is incorporated herein by reference in its entirety for any and all purposes.FIELDThe disclosure relates to an emulsifier system comprising peptide compositions, e.g., silk fibroin derived peptide compositions.BACKGROUNDSilk is a natural polymer produced by a variety of insects and spiders, and comprises a filament core protein, silk fibroin, and a glue-like coating consisting of a non-filamentous protein, sericin.SUMMARYThe disclosure provides an emulsifier system comprising peptide compositions, e.g., any silk fibroin derived peptide compositions described herein, whether comprising modifications, substitutions, or replacements, or not.The disclosure provides an emulsifier system comprising a peptide or protein fragment comprising a plurality of amino acids selected from M, R, V, K, T, F, I, L, C, A, Q, Y, N, D, E, G, S, H, P, and W, wherein at least one of the amino acids is modified, substituted, or replaced. In some embodiments, the peptide or protein fragment is a fibroin peptide or protein fragment comprising an amino acid modification, substitution, or replacement of an amino acid from of amino acids selected from M, R, V, K, T, F, I, L, C, A, Q, Y, N, D, E, G, S, H, P, and W. In some embodiments, the fibroin is a fibroin heavy chain, a fibroin light chain, or a fibrohexamerin. In some embodiments, the peptide or protein fragment comprises between about 2 and about 100 amino acids. In some embodiments, the peptide orprotein fragment comprises between about 2 and about 25 amino acids. In some embodiments, the peptide or protein fragment comprises between about 25 and about 50 ammo acids. In some embodiments, the peptide or protein fragment comprises between about 50 and about 75 amino acids. In some embodiments, the peptide or protein fragment comprises between about 75 and about 100 amino acids. In some embodiments, the peptide or protein fragment comprises between about 100 and about 125 amino acids. In some embodiments, the peptide or protein fragment comprises between about 125 and about 150 amino acids. In some embodiments, the peptide or protein fragment comprises between about 150 and about 200 amino acids. In some embodiments, the peptide or protein fragment comprises between about 200 and about 250, 300. 350, 400, 450, or 500 amino acids. In some embodiments, the peptide or protein fragment comprises between one and five modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises one modification, substitution, and / or replacement. In some embodiments, the peptide or protein fragment comprises two modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises three modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises four modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises five modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises six modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises seven modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises eight modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment compnses nine modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises ten modifications, substitutions, and / or replacements. In some embodiments, a modification, substitution, and / or replacement is selected from an asparagine to aspartic acid modification, substitution, and / or replacement, a glutamine to glutamic acid modification, substitution, and / or replacement, and a methionine to methionine oxide modification, substitution, and / or replacement. In some embodiments, the fibroin is a fibroin heavy chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 5263 of the fibroin heavy chain. In some embodiments, a modification.substitution, and / or replacement is at Q58, M64. N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275, N4191. Q5216, and / or N5262 position of fibroin heavy chain. In some embodiments, the fibroin is a fibroin light chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 262 of the fibroin light chain. In some embodiments, a modification, substitution, and / or replacement is at N23, Q24, N28, M69. N105, N108, N118. N136, N138. Q149, N186. N200, Q202. N204, N240. N248, and / or Q255 position of fibroin light chain. In some embodiments, the fibroin is a fibrohexamerin (p25), and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 220 of the fibrohexamerin (p25). In some embodiments, a modification, substitution, and / or replacement is at Q62, N93, M120, N149, N172, N174, and / or N202 position of fibrohexamerin (p25).The disclosure provides an emulsifier system comprising a composition comprising a plurality of peptides or protein fragments, each comprising a plurality of amino acids selected from M, R, V, K. T, F, I, L, C, A, Q. Y, N, D, E, G, S, H, P. and W, wherein at least one of the amino acids is modified, substituted, or replaced. In some embodiments, the plurality of peptides or protein fragments comprises a fibroin peptide or protein fragment comprising an amino acid modification, substitution, or replacement of an amino acid selected from M, R, V, K, T, F, I, L, C, A, Q. Y, N, D, E, G, S, H, P, and W. In some embodiments, the fibroin is a fibroin heavy chain, a fibroin light chain, or a fibrohexamerin. In some embodiments, the peptide or protein fragment comprises between about 2 and about 100 amino acids. In some embodiments, the peptide or protein fragment comprises between about 2 and about 25 amino acids. In some embodiments, the peptide or protein fragment comprises between about 25 and about 50 amino acids. In some embodiments, the peptide or protein fragment comprises between about 50 and about 75 amino acids. In some embodiments, the peptide or protein fragment comprises between about 75 and about 100 amino acids. In some embodiments, the peptide or protein fragment comprises between about 100 and about 125 amino acids. In some embodiments, the peptide or protein fragment comprises between about 125 and about 150 amino acids. In some embodiments, the peptide or protein fragment comprises between about 150 and about 200 amino acids. In some embodiments, the peptide or protein fragment comprises between about 200 and about 250, 300, 350. 400, 450, or 500 amino acids. In some embodiments, the peptide or protein fragment compnses between one and fivemodifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises one modification, substitution, and / or replacement. In some embodiments, the peptide or protein fragment comprises two modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises three modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises four modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises five modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises six modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises seven modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises eight modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises nine modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises ten modifications, substitutions, and / or replacements. In some embodiments, a modification, substitution, and / or replacement is selected from an asparagine to aspartic acid modification, substitution, and / or replacement, a glutamine to glutamic acid modification, substitution, and / or replacement, and a methionine to methionine oxide modification, substitution, and / or replacement. In some embodiments, the fibroin is a fibroin heavy chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 5263 of the fibroin heavy chain. In some embodiments, a modification, substitution, and / or replacement is at Q58, M64, N68, N70, N77, M80, N93, M103. Q125, N132. Q139, Q275. N4191, Q5216, and / or N5262 position of fibroin heavy chain. In some embodiments, the fibroin is a fibroin light chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 262 of the fibroin light chain. In some embodiments, a modification, substitution, and / or replacement is at N23, Q24, N28, M69, N105. N108, N118. N136, N138. Q149, N186, N200, Q202, N204, N240, N248, and / or Q255 position of fibroin light chain. In some embodiments, the fibroin is a fibrohexamerin (p25), and wherein a modification, substitution, and / or replacement is at a position corresponding to anyone position from 1 to 220 of the fibrohexamerin (p25). In some embodiments, a modification, substitution, and / or replacement is at Q62, N93, M120, N149, N172,N174, and / or N202 position of fibrohexamerin (p25). In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 1% to about 99%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 1% to about 10%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 10% to about 20%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 20% to about 30%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 30% to about 40%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 40% to about 50%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 50% to about 60%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 60% to about 70%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 70% to about 80%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 80% to about 90%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between or about 90% to about 99%. As used herein, a % modification, substitution, and / or replacement is defined as (number of peptide or protein fragments comprising a modification, substitution, and / or replacement at a specific position, divided by the total number of peptide or protein fragments which include the specific position, whether comprising a modification, substitution, and / or replacement, or not) x 100.The disclosure provides an emulsifier system comprising a composition comprising a plurality of peptides or protein fragments of fibroin heavy chain, fibroin light chain, and / or fibrohexamerin (p25), the composition comprising one or more fractions, wherein the plurality of peptides or protein fragments comprises a fibroin peptide or protein fragment comprising an amino acid modification, substitution, or replacement. In some embodiments, the plurality of peptides or protein fragmentshaving a weight average molecular weight (Mw) selected from between about 1 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa. from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, or from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 250 kDa, and a poly dispersity between 1 and about 1.7, between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 10 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, or from between about 160 kDa and about 180 kDa. and a poly dispersity between 1 and about 1. 1. or 1 and about 1.2, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 10 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa. from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, or from between about 120 kDa and about 140 kDa, and a poly dispersity between 1 and about 1.1, or 1 and about 1.2, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, or from between about 100 kDa and about 120 kDa, and a poly dispersity between 1 and about 1.1. or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 10 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from betw een about 60 kDa and about 80 kDa. from between about 80 kDa and about 100 kDa, or from between about 100 kDa and about 110 kDa, and apolydispersity between 1 and about 1.1, or 1 and about 1.2, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, or from between about 120 kDa and about 140 kDa, and a poly dispersity between 1 and about 1.1. or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 20 kDa and about 40 kDa, or from between about 40 kDa and about 60 kDa, and a polydispersity between 1 and about 1.1, or 1 and about 1.2, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the one or more fractions are selected from AS77, AS78, AS79, AS80, and AS81. In some embodiments, the one or more fractions are selected from AS82, AS83, AS84, AS85, AS86, AS87, AS88, and AS89. In some embodiments, the one or more fractions are selected from AS90, AS91. AS 92, AS93, and AS94. In some embodiments, the one or more fractions are selected from AS95, AS96, AS97, AS98, AS99, and AS 100. In some embodiments, the plurality of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 220 kDa, and a poly dispersity between 1 and about 1.7. or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 210 kDa, and a poly dispersity between 1 and about 1.2, or 1 and about 1.3, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in afraction having a weight average molecular weight (Mw) selected from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, or from between about 100 kDa and about 110 kDa, and a poly dispersity between 1 and about 1.1, or 1 and about 1.2, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 60 kDa and about 80 kDa. from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 210 kDa, and a polydispersity between 1 and about 1.2, or 1 and about 1.3, or between 1 and less than 3, or between 1 and less than 5. In some embodiments, the one or more fractions are selected from AS101, AS102, AS103, AS104, and AS105. In some embodiments, the one or more fractions are selected from AS 106, AS 107, AS 108. AS 109, AS 110, and AS111. In some embodiments, an amino acid is selected from M, R, V, K, T, F, I, L, C, A, Q, Y, N, D, E, G, S, H, P, and W. In some embodiments, a peptide or protein fragment comprises between about 2 and about 100 amino acids. In some embodiments, the peptide or protein fragment comprises between about 2 and about 25 amino acids. In some embodiments, the peptide or protein fragment comprises between about 25 and about 50 amino acids. In some embodiments, the peptide or protein fragment comprises between about 50 and about 75 amino acids. In some embodiments, the peptide or protein fragment comprises between about 75 and about 100 amino acids. In some embodiments, the peptide or protein fragment compnses between about 100 and about 125 amino acids. In some embodiments, the peptide or protein fragment comprises between about 125 and about 150 amino acids. In some embodiments, the peptide or protein fragment comprises between about 150 and about 200 amino acids. In some embodiments, the peptide or protein fragment comprises between about 200 and about 250, 300. 350, 400. 450, or 500 amino acids. In some embodiments, a peptide or protein fragment comprises between one and five modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises one modification, substitution, and / or replacement. In some embodiments, the peptide or protein fragment comprises two modifications, substitutions, and / or replacements. In someembodiments, the peptide or protein fragment comprises three modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises four modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises five modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises six modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises seven modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises eight modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises nine modifications, substitutions, and / or replacements. In some embodiments, the peptide or protein fragment comprises ten modifications, substitutions, and / or replacements. In some embodiments, the fibroin is a fibroin heavy chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 5263 of the fibroin heavy chain. In some embodiments, the fibroin is a fibroin light chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 262 of the fibroin light chain. In some embodiments, the fibroin is a fibrohexamerin (p25) chain, and wherein a modification, substitution, and / or replacement is at a position corresponding to any one position from 1 to 220 of the fibrohexamerin (p25) chain. In some embodiments, a modification, substitution, and / or replacement is selected from an asparagine to aspartic acid modification, substitution, and / or replacement, a glutamine to glutamic acid modification, substitution, and / or replacement, and a methionine to methionine oxide modification, substitution, and / or replacement. In some embodiments, a modification, substitution, and / or replacement is at fibroin heavy chain position selected from Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, and / or N5262. In some embodiments, a modification, substitution, and / or replacement is at fibroin light chain position selected from N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, and / or Q255. In some embodiments, a modification, substitution, and / or replacement is at fibrohexamerin (p25) position selected from Q62, N93, M120, N149, N172, N174, and / or N202. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 1% to about 99%. In some embodiments, each modification, substitution,and / or replacement is independently ranging in the composition between about 1% to about 10%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 10% to about 20%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 20% to about 30%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 30% to about 40%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 40% to about 50%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 50% to about 60%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 60% to about 70%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 70% to about 80%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between about 80% to about 90%. In some embodiments, each modification, substitution, and / or replacement is independently ranging in the composition between or about 90% to about 99%. As used herein, a % modification, substitution, and / or replacement is defined as (number of peptide or protein fragments comprising a modification, substitution, and / or replacement at a specific position, divided by the total number of peptide or protein fragments which include the specific position, whether comprising a modification, substitution, and / or replacement, or not) x 100. In some embodiments, a molecular weight is determined by MALS.The disclosure provides an article comprising one or more peptides or protein fragments disclosed herein, and / or one or more compositions disclosed herein. A composition may include unmodified fibroin peptides and protein fragments. An article is, without limitation, selected from a personal care article disclosed herein. The disclosure provides an article comprising one or more peptides or protein fragments disclosed herein, and / or one or more compositions disclosed herein, and a substrate or any other support or combination element disclosed herein.In some embodiments, the disclosure provides an emulsifier system comprising a composition disclosed herein comprising a plurality of peptides or protein fragments, e.g., a plurality of fibroin heavy chain peptides or fibroin heavychain fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, and further comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: a ratio of Q58 to M64 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of Q58 to N68 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q58 to N70 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q58 to N77 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q58 to M80 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q58 to N93 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25;a ratio of Q58 to M103 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q58 to QI 25 modifications, substitutions, and / or replacements of about 25: 1. about 20:1. about 15:1, about 10:1, about 9:1. about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q58 to N132 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q58 to QI 39 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of Q58 to Q275 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q58 to N4191 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q58 to Q5216 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of Q58 to N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of M64 to N68 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M64 to N70 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20, or about 1:25; a ratio of M64 to N77 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M64 to M80 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M64 to N93 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M64 to M103 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M64 to Q125 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of M64 to N132 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M64 to QI 39 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M64 to Q275 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M64 to N4191 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M64 to Q5216 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25;a ratio of M64 to N5262 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N68 to N70 modifications, substitutions, and / or replacements of about 25: 1. about 20:1. about 15:1, about 10:1, about 9:1. about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N68 to N77 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N68 to M80 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N68 to N93 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N68 to M103 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N68 to Q125 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N68 to N132 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N68 to QI 39 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N68 to Q275 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20, or about 1:25; a ratio of N68 to N4191 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N68 to Q5216 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N68 to N5262 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N70 to N77 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N70 to M80 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of N70 to N93 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N70 to M103 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N70 to Q125 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N70 to N132 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N70 to Q139 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25;a ratio of N70 to Q275 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N70 to N4191 modifications, substitutions, and / or replacements of about 25: 1. about 20:1. about 15:1, about 10:1, about 9:1. about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N70 to Q5216 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N70 to N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N77 to M80 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N77 to N93 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N77 to Ml 03 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N77 to Q125 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N77 to N132 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N77 to Q139 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20, or about 1:25; a ratio of N77 to Q275 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N77 to N4191 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N77 to Q5216 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N77 to N5262 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M80 to N93 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of M80 to Ml 03 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M80 to Q125 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M80 to N132 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M80 to QI 39 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M80 to Q275 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25;a ratio of M80 to N4191 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M80 to Q5216 modifications, substitutions, and / or replacements of about 25: 1. about 20:1. about 15:1, about 10:1, about 9:1. about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M80 to N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N93 to M103 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N93 to Q125 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N93 to N132 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N93 to Q139 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N93 to Q275 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N93 to N4191 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N93 to Q5216 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20, or about 1:25; a ratio of N93 to N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M103 to Q125 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M103 to N132 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M103 to Q139 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M103 to Q275 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of M103 to N4191 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Ml 03 to Q5216 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Ml 03 to N5262 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q125 to N132 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q125 to Q139 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25;a ratio of Q125 to Q275 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q125 to N4191 modifications, substitutions, and / or replacements of about 25: 1. about 20:1. about 15:1, about 10:1, about 9:1. about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q125 to Q5216 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q125 to N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N132 to Q139 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N132 to Q275 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N132 to N4191 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N132 to Q5216 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N 132 to N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q139 to Q275 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20, or about 1:25; a ratio of QI 39 to N4191 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q139 to Q5216 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q139 to N5262 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q275 to N4191 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q275 to Q5216 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of Q275 to N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N4191 to Q5216 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N4191 to N5262 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q5216 to N5262 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25;In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of fibroin heavy chain and light chain peptides or fibroin heavy chain and light chain fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, and further comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from:a ratio of heavy chain Q58 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149,N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20: 1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6: 1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain M64 to light chain N23. Q24. N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy7chain N68 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149,N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1. about 20:1, about 15:1. about 10:1. about 9:1, about 8:1. about 7:1, about 6: 1. about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain N70 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149,N186, N200, Q202, N204. N240, N248. or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6: 1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain N77 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy7chain M80 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149,N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1. about 20: 1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6: 1, about 5:1, about 4:1,about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain N93 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149,N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain M103 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1. about 20: 1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6: 1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain Q125 to light chain N23, Q24, N28. M69, N105, N108, N118, N136. N138, Q149. N186, N200. Q202, N204. N240, N248. or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain N132 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149,N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy7chain QI 39 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1. about 20: 1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6: 1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain Q275 to light chain N23, Q24, N28. M69, N105, N108, N118, N136, N138, Q149,N186, N200, Q202, N204, N240, N248, or Q255modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain N4191 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1. about 20: 1, about 15:1, about 10:1, about 9: 1 , about 8:1, about 7:1, about 6: 1 , about 5:1, about 4: 1 , about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain Q5216 to light chain N23. Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain N5262 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1. about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25.In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of fibroin heavy chain and p25 peptides or fibroin heavy chain and p25 fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: a ratio of heavy chain Q58 to p25 Q62, N93. M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25;a ratio of heavy chain M64 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15: 1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain N68 to p25 Q62, N93. M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25: 1, about 20: 1, about 15: 1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain N70 to p25 Q62, N93. M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain N77 to p25 Q62, N93. M120. N149, N172. N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of heavy chain M80 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain N93 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1. about 9:1, about 8:1. about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain M103 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1. about 10:1. about 9: 1, about 8:1. about 7:1, about 6:1. about 5:1, about4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain Q125 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20. or about 1:25; a ratio of heavy chain N132 to p25 Q62, N93, Ml 20, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain Q139 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1. about 9: 1, about 8:1. about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain Q275 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1. about 10:1. about 9: 1, about 8:1. about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain N4191 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15: 1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain Q5216 to p25 Q62, N93, M120. N149, N172. N174, or N202 modifications, substitutions, and / or replacements of about 25: 1, about 20: 1, about 15: 1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of heavy chain N5262 to p25 Q62, N93, M120. N149, N172. N174, or N202 modifications, substitutions, and / or replacements of about 25: 1, about 20: 1,about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25.In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of light chain fibroin peptides or fibroin fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: a ratio of N23 to Q24 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q24 to N28 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q24 to M69 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q24 to N 105 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q24 to N 108 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25;a ratio of Q24 to N118 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q24 to N 136 modifications, substitutions, and / or replacements of about 25: 1. about 20:1. about 15:1, about 10:1, about 9:1. about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q24 to N 138 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q24 to QI 49 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of Q24 to N186 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q24 to N200 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q24 to Q202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of Q24 to N204 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of Q24 to N240 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q24 to N248 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20, or about 1:25; a ratio of Q24 to Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N28 to M69 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N28 to N105 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N28 to N108 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N28 to N118 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of N28 to N136 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N28 to N138 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N28 to Q149 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N28 to N186 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N28 to N200 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25;a ratio of N28 to Q202 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N28 to N204 modifications, substitutions, and / or replacements of about 25: 1. about 20:1. about 15:1, about 10:1, about 9:1. about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N28 to N240 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N28 to N248 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N28 to Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M69 to N105 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M69 to N108 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of M69 to N 118 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of M69 to N136 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M69 to N138 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20, or about 1:25; a ratio of M69 to Q149 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M69 to N186 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M69 to N200 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M69 to Q202 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M69 to N204 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of M69 to N240 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M69 to N248 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M69 to Q255 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N105 to N108 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N105 to N118 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25;a ratio of N105 to N136 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N105 to N138 modifications, substitutions, and / or replacements of about 25: 1. about 20:1. about 15:1, about 10:1, about 9:1. about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N105 to Q149 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N 105 to N 186 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N105 to N200 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N 105 to Q202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N105 to N204 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N105 to N240 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N 105 to N248 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N105 to Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20, or about 1:25; a ratio ofN108 to N118 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N108 to N136 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N108 to N138 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N108 to Q149 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N 108 to N186 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of N108 to N200 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N108 to Q202 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N108 to N204 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N108 to N240 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N108 to N248 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25;a ratio of N108 to Q255 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N118 to N136 modifications, substitutions, and / or replacements of about 25: 1. about 20:1. about 15:1, about 10:1, about 9:1. about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N118 to N138 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N 118 to Q149 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N118 to N186 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N 118 to N200 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N118 to Q202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N118 to N204 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N 118 to N240 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N118 to N248 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20, or about 1:25; a ratio of N118 to Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N136 to N138 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N136 to Q149 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N136 to N186 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N 136 to N200 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of N136 to Q202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N136 to N204 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N136 to N240 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N136 to N248 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N136 to Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25;a ratio of N138 to Q149 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N138 to N186 modifications, substitutions, and / or replacements of about 25: 1. about 20:1. about 15:1, about 10:1, about 9:1. about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N138 to N200 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N 138 to Q202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N138 to N204 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N 138 to N240 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N138 to N248 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N138 to Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of Q149 to N186 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q149 to N200 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20, or about 1:25; a ratio of Q149 to Q202 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q149 to N204 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q149 to N240 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q149 to N248 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q149 to Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of N186 to N200 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N186 to Q202 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N186 to N204 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N186 to N240 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N186 to N248 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25;a ratio of N186 to Q255 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N200 to Q202 modifications, substitutions, and / or replacements of about 25: 1. about 20:1. about 15:1, about 10:1, about 9:1. about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N200 to N204 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N200 to N240 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of N200 to N248 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N200 to Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q202 to N204 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of Q202 to N240 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of Q202 to N248 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q202 to Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20, or about 1:25; a ratio of N204 to N240 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N204 to N248 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N204 to Q255 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N240 to N248 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N240 to Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of N248 to Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25;In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of fibroin light chain and heavy chain peptides or fibroin light chain and heavy chain fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, and further comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: a ratio of light chain N23 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7: 1, about 6:1, about 5:1, about 4: 1, about 3: 1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain Q24 to heavy chain Q58, M64, N68, N70, N77, M80, N93,M103, Q125, N132, Q139, Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7: 1, about 6:1, about 5:1, about 4:1, about 3: 1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N28 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139. Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1,about 9:1, about 8:1, about 7: 1, about 6:1, about 5:1, about 4: 1, about 3: 1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain M69 to heavy chain Q58, M64, N68, N70, N77, M80, N93,M103, Q125, N132, Q139, Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8: 1, about 7: 1, about 6:1, about 5:1, about 4: 1, about 3: 1, about 2:1, about 1:1, about 1 :2, about 1:3, about 1 :4, about 1 :5, about 1 :6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N105 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125.N132, Q139. Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N108 to heavy chain Q58, M64, N68, N70, N77. M80. N93, M103, Q125, N132, Q139, Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N118 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N136 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125. N132, Q139. Q275, N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25;a ratio of light chain N138 to heavy chain Q58, M64, N68, N70, N77, M80. N93, M103, Q125, N132, Q139. Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain Q149 to heavy chain Q58, M64, N68, N70, N77. M80, N93, Ml 03, QI 25, N132, QI 39, Q275,N4191, Q5216, orN5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7: 1, about 6:1, about 5:1, about 4: 1, about 3: 1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N186 to heavy chain Q58, M64, N68, N70, N77, M80, N93,M103, Q125, N132, Q139, Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1. about 7:1, about 6:1, about 5:1, about 4:1, about 3: 1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N200 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125.N132, Q139. Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain Q202 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N204 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20: 1, about 15: 1, about 10: 1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1,about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N240 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1 :9, about 1:10, about 1:15, about 1 :20, or about 1 :25; a ratio of light chain N248 to heavy chain Q58, M64, N68, N70, N77, M80, N93,M103, Q125, N132, Q139, Q275,N4191, Q5216, or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20: 1, about 15: 1, about 10: 1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain Q255 to heavy chain Q58, M64, N68, N70, N77. M80, N93, M103, Q125. N132, Q139. Q275, N4191, Q5216. or N5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8: 1, about 7: 1, about 6:1, about 5:1, about 4:1, about 3: 1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25.In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of fibroin light chain and p25 peptides or fibroin light chain and p25 fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: a ratio of light chain N23 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1. about 10:1. about 9: 1, about 8:1. about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain Q24 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15: 1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N28 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20. or about 1:25; a ratio of light chain M69 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N105 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1. about 9: 1, about 8:1. about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N108 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1. about 10:1. about 9: 1, about 8:1. about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain Nil 8 to p25 Q62. N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15: 1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N136 to p25 Q62. N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25: 1, about 20: 1, about 15: 1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N138 to p25 Q62. N93. M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25: 1, about 20: 1,about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain Q149 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1. about 9:1, about 8:1. about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1 :6, about 1 :7, about 1 :8, about 1 :9, about 1:10, about 1:15, about 1 :20, or about 1 :25; a ratio of light chain N186 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1. about 10:1. about 9: 1, about 8:1. about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N200 to p25 Q62, N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15: 1. about 10:1. about 9:1, about 8:1. about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain Q202 to p25 Q62. N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25: 1, about 20: 1, about 15: 1, about 10:1, about 9: 1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N204 to p25 Q62. N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25: 1, about 20: 1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain N240 to p25 Q62. N93. M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25;a ratio of light chain N248 to p25 Q62. N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15: 1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of light chain Q255 to p25 Q62. N93, M120, N149, N172, N174, or N202 modifications, substitutions, and / or replacements of about 25: 1, about 20: 1, about 15: 1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25.In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of p25 fibroin peptides or fibroin fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: a ratio of Q62 to N93 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of Q62 to M120 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q62 to N149 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of Q62 to N172 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of Q62 to N 174 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25; a ratio of Q62 to N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N93 to M120 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2. about 1:3, about 1:4. about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15. about 1:20, or about 1:25; a ratio of N93 to N149 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N93 to N172 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N93 to N174 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N93 to N202 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about6:1. about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M120 to N149 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about2:l. about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8. about 1:9, about 1:10, about 1:15, about 1 :20, or about 1:25; a ratio of M120 to N172 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M120 to N174 modifications, substitutions, and / or replacements of about 25:1, about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1. about 5:1, about 4:1. about 3:1, about 2:1. about 1:1, about 1:2. about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of M120 to N202 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N149 to N172 modifications, substitutions, and / or replacements of about 25: 1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N149 to N174 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4. about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25;a ratio of N149 to N202 modifications, substitutions, and / or replacements of about 25:1. about 20:1. about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N172 to N174 modifications, substitutions, and / or replacements of about 25: 1. about 20:1. about 15:1, about 10:1, about 9:1. about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N172 to N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of N 174 to N202 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6. about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20. or about 1:25.In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of p25 and heavy chain peptides or p25 and heavy chain fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: a ratio of p25 Q62 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, orN5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7: 1, about 6:1, about 5:1, about 4:1, about 3: 1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of p25 N93 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125. N132, Q139. Q275, N4191, Q5216, orN5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1,about 9:1, about 8:1, about 7: 1, about 6:1, about 5:1, about 4: 1, about 3: 1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of p25 Ml 20 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, orN5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8: 1, about 7: 1, about 6:1, about 5:1, about 4: 1, about 3: 1, about 2:1, about 1:1, about 1 :2, about 1:3, about 1 :4, about 1 :5, about 1 :6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of p25 N149 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125. N132, Q139. Q275, N4191, Q5216. orN5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of p25 N172 to heavy chain Q58. M64. N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, orN5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of p25 N174 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125, N132, Q139, Q275, N4191, Q5216, orN5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of p25 N202 to heavy chain Q58, M64, N68, N70, N77, M80, N93, M103, Q125. N132, Q139. Q275, N4191, Q5216. orN5262 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25.In some embodiments, the disclosure provides a composition comprising a plurality of peptides or protein fragments, e.g., a plurality of p25 and light chain peptides or fibroin light chain and light chain fragments, and comprising a plurality of amino acid modifications, substitutions, and / or replacements, and further comprising one or more ratios of modifications, substitutions, and / or replacements at specific positions selected from: aratioofp25 Q62 to light chain N23, Q24,N28, M69, N105, N108, N118. N136, N138, QI 49, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7: 1, about 6:1, about 5:1, about 4: 1, about 3: 1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of p25 N93 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1. about 7:1, about 6:1, about 5:1, about 4:1, about 3: 1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of p25 M120 to light chain N23, Q24, N28, M69, N105, N108, Nil 8, N136, N138, Q149, N186, N200, Q202, N204, N240. N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio ofp25N149 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20:1, about 15:1, about 10:1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1, about 1:1, about 1:2, about 1:3, about 1:4, about 1:5, about 1:6, about 1:7, about 1:8, about 1:9, about 1:10, about 1:15, about 1:20, or about 1:25; a ratio of p25 N172 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25:1, about 20: 1, about 15: 1, about 10: 1, about 9:1, about 8:1, about 7:1, about 6:1, about 5:1, about 4:1, about 3:1, about 2:1,about 1: 1, about 1:2, about 1:3, about 1:4, about 1 :5, about 1 :6, about 1:7, about 1:8, about 1:9, about 1 : 10, about 1 : 15, about 1 :20, or about 1 :25; a ratio of p25 N 174 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25: 1, about 20: 1, about 15: 1, about 10: 1, about 9: 1, about 8: 1, about 7: 1, about 6: 1, about 5: 1, about 4: 1, about 3: 1, about 2: 1, about 1: 1, about 1 :2, about 1:3, about 1:4, about 1 :5, about 1 :6, about 1:7, about 1:8, about 1 :9, about 1 : 10, about 1 : 15, about 1 :20, or about 1 :25; a ratio of p25 N202 to light chain N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, or Q255 modifications, substitutions, and / or replacements of about 25: 1, about 20: 1, about 15: 1, about 10: 1, about 9: 1, about 8: 1, about 7: 1, about 6: 1, about 5: 1, about 4: 1, about 3: 1, about 2: 1, about 1 : 1, about 1 :2, about 1:3, about 1:4, about 1 :5, about 1 :6, about 1:7, about 1:8, about 1:9, about 1 : 10, about 1 :15, about 1 :20, or about 1 :25.In some embodiments, the disclosure further provides an emulsifier system comprising a composition comprising peptides or protein fragments described herein, and a thickening or stabilizer agent, wherein the thickening or stabilizer agent comprises a polysaccharide, a nanoclay, or both. In some embodiments, the polysaccharide is xanthan gum. In some embodiments, the emulsifier system comprises one or more components selected from an aqueous phase, an oil phase, a preservative, a humectant, and a pH adjuster.In some embodiments, the disclosure further provides personal care and / or cosmetic formulations and / or compositions comprising silk fibroin fragments having an average weight average molecular weight selected from between about 1 kDa and about 5 kDa, between about 5 kDa and about 10 kDa, between about 6 kDa and about 17 kDa, between about 10 kDa and about 15 kDa, between about 15 kDa and about 20 kDa, between about 14 kDa and about 30 kDa, between about 17 kDa and about 39 kDa, between about 20 kDa and about 25 kDa, between about 25 kDa and about 30 kDa, between about 30 kDa and about 35 kDa, between about 35 kDa and about 40 kDa, between about 39 kDa and about 54 kDa, between about 39 kDa and about 80 kDa, between about 40 kDa and about 45 kDa, between about 45 kDa and about 50 kDa, between about 60 kDa and about 100 kDa, and between about 80 kDa and about 144 kDa, and a poly dispersity between 1 and about 5, and without limitation, can also comprise modified silk fragments disclosed herein, e.g., and without limitation.fibroin peptides and / or protein fragments wherein at least one of the amino acids is modified, substituted, or replaced as disclosed herein. In some embodiments, the composition further comprises 0 to 500 ppm lithium bromide. In some embodiments, the composition further comprises 0 to 500 ppm sodium carbonate. In some embodiments, the silk fibroin fragments have a polydispersity between 1 and about 1.5. In some embodiments, the silk fibroin fragments have a poly dispersity between about 1.5 and about 2.0. In some embodiments, the silk fibroin fragments have a poly dispersity between about 1.5 and about 3.0. In some embodiments, the silk fibroin fragments have a poly dispersity between about 2.0 and about 2.5. In some embodiments, the silk fibroin fragments have a polydispersity between about 2.5 and about 3.0. In some embodiments, the silk fibroin fragments are present in the composition at about 0.001 wt. % to about 10.0 wt. % relative to the total weight of the composition. In some embodiments, the composition further comprises about 0.001% (w / w) to about 10% (w / w) sericin relative to the total weight of the composition. In some embodiments, the composition further comprises about 0.001% (w / w) to about 10% (w / w) sericin relative to the silk fibroin fragments. In some embodiments, the silk fibroin fragments do not spontaneously or gradually gelate and do not visibly change in color or turbidity when in an aqueous solution for at least 10 days prior to formulation into the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 0.01 wt. % to about 10.0 wt. % relative to the total weight of the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 0.01 wt. % to about 1.0 wt. % relative to the total weight of the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 1.0 wt. % to about 2.0 wt. % relative to the total weight of the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 2.0 wt. % to about 3.0 wt. % relative to the total weight of the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 3.0 wt. % to about 4.0 wt. % relative to the total weight of the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 4.0 wt. % to about 5.0 wt. % relative to the total w eight of the composition. In some embodiments, the silk fibroin fragments are present in the composition at about 5.0 wt. % to about 6.0 wt. % relative to the total weight of the composition.In some embodiments, the composition is formulated as a topical composition. In some embodiments, the composition further comprises a pharmaceutically acceptable carrier. In some embodiments, the composition further comprises a dermatologically acceptable carrier. In some embodiments, the pharmaceutically acceptable carrier comprises one or more of a suspension, an emulsion, a powder, a solution, a dispersion, or an elixir. In some embodiments, the pharmaceutically acceptable carrier comprises or is formulated as one or more of a gel. a jelly, a cream, a lotion, a foam, a slurry, an ointment, an oil, a paste, a suppository, a spray, a semisolid composition, a solid composition, a stick, or a mousse. In some embodiments, the pharmaceutically acceptable carrier comprises one or more of sesame oil, com oil, cottonseed oil, or peanut oil. In some embodiments, the pharmaceutically acceptable carrier comprises one or more of mannitol or dextrose. In some embodiments, the pharmaceutically acceptable carrier comprises free and / or uncrosslinked hyaluronic acid. In some embodiments, the pharmaceutically acceptable carrier comprises one or more of aliphatic oil, a fatty alcohol, a fatty acid, a glyceride, an acylglycerol, and a phospholipid. In some embodiments, the pharmaceutically acceptable carrier comprises one or more of a monoglyceride, a diglyceride, or a triglyceride. In some embodiments, the pharmaceutically acceptable carrier comprises an aqueous phase. In some embodiments, the pharmaceutically acceptable carrier comprises an oil-in-water emulsion or a water-in-oil emulsion. In some embodiments, the pharmaceutically acceptable carrier comprises one or more of a hydrocarbon oil, a fatty acid, a fatty oil, a fatty acid ester, or a cationic quaternary ammonium salt.BRIEF DESCRIPTION OF THE DRAWINGSThe presently disclosed embodiments will be further explained with reference to the attached drawings. The drawings shown are not necessarily to scale, with emphasis instead generally being placed upon illustrating the principles of the presently disclosed embodiments.Figure 1. Ion Exchange Fractionation Schemes for the isolation of the populations that constitute Low' and Mid Skid silk / modified polypeptide compositions. Low and Mid Skid silk / modified polypeptide compositions contains silk / modified polypeptides that are negatively, positively charged, or neutral. Using Q anion exchange chromatography (A) these populations were isolated.Figure 2. Chromatogram of Low Skid silk / modified polypeptide composition loaded in a Q-Sepharose HP column (Cytiva). The flow through contains the silk / modified polypeptides that do not get captured in the column and are the depleted in negatively charged amino acids. After the column is loaded with Low or Mid Skid silk / modified polypeptide compositions and the flow through is collected, the column is washed until the UV-280 absorbance becomes less than 200 AU. The captured negatively charged silk / modified polypeptides are eluted with high salt concentration (1M NaCl) and constitute AS1 1 and AS22. The chromatography is performed in Tris- containing buffers but the flow7through and the Q-elution were finally dialyzed in water.Figure 3. Analytical Size Exclusion Chromatography of Low, Mid Skid silk / modified silk compositions and their constituent AS compositions. Average molecular weight in kDa and poly dispersity measurements are shown.Figures 4A- 4B. Analytical Size Exclusion Chromatography of the Low' and Mid skid silk / modified peptide compositions and their components (see table 1 for more details). Fig. 4A, Molecular weight of the various Activated Silk new compositions described in this study. Fig. 4B, Poly dispersity (PDI) of the various Activated Silk new compositions described in this study. AS24 reconstitutes the average molecular weight and polydispersity of the Low- skid silk / modified peptide composition and it consists of 50% AS 12 and 50% AS22 (see table 1 for details). AS6 reconstitutes the average molecular weight and polydispersity of the Mid skid silk / modified peptide composition and it consists of 50% AS1 and 50% AS11 (see table 1 for details).Figure 5. Isoelectric Focusing Electrophoresis of Low Skid silk / modified polypeptide compositions. Lanes 2, 7; Low Skid silk different amounts loaded. Lanes 3, 5, 8, 10; AS12 silk, different preparations different amounts loaded. Lanes 4, 6, 9, 11; AS22 silk, different preparations different amounts loaded.Figures 6A- 6B. Self-assembly reactions of the of the Low and Mid skid silk / modified peptide compositions and their components (see table 1 for more details). Both graphs depict the kinetic parameters of gel formation during self- assembly of silk. On graph A calculation of the three self-assembly kinetic parameters is shown, t0.5, Amax and SARF. For more details look at the text.Figures 7A- 7C. Self-assembly kinetics of the Low and Mid skid silk / modified peptide compositions and their components (see table 1 for moredetails). Fig. 7A, the Self-assembly Rate Factor shows how fast the self-assembly reaction proceeds once it is initiated and the self-assembly nuclei are organized. Fig. 7B, Maximum Gel Yield shows how dense the silk gel is after self-assembly is complete. Fig. 7C, Time required for the self-assembly reaction to produce half of the maximum gel amount.Figure 8. the Low and Mid skid silk / modified peptide compositions and their components (see table 1 for more details). The Self Assembly Factor reflects the average propensity of silk to self-assemble and form gels.While the above-identified drawings set forth presently disclosed embodiments, other embodiments are also contemplated, as noted in the discussion. This disclosure presents illustrative embodiments by way of representation and not limitation. Numerous other modifications and embodiments can be devised by those skilled in the art which fall within the scope and spirit of the principles of the presently disclosed embodiments.Figure 9 is a graph of Weight average molecular weight (i.e., average molecular weight average or average MW) using Size exclusion chromatography with a refractive index detector (SEC-RI) plotted as a function of time for solubilized fibroin in 9.3 M LiBr at 100 °C - 103 °C (i.e., 3 degree Celsius temperature gradient between 100 °C and 103 °C).Figure 10 is a graph of weight average molecular weight (i.e., average molecular weight average or average MW) using Size exclusion chromatography with a refractive index detector (SEC-RI) plotted as a function of time for solubilized fibroin in 9.3 M LiBr at 122 °C - 125 °C (i.e., 3 degree Celsius temperature gradient between 122 °C and 125 °C).Figure 11 is a graph illustrating percentage of amino acid modification in silk.Figures 12A-12C are graphs illustrating percentage of amino acid modifications in Low Skid Silk and Mid Skid silk. Fig. 12A illustrates heavy chain modifications, Fig. 12B illustrates light chain modifications, and Fig. 12C illustrates fibrohexamerin modifications. N are Asparagines that become aspartic acid and Q are Glutamines that become deamidated. M corresponds to Methionies that become oxidized. The numbers after each amino acid show its position along the amino acid chain from the corresponding protein.Figures 13A- 13B are graphs illustrating percentage of amino acid modifications in Low Skid Silk and Mid Skid silk produced and lyophilized. Fig. 13A illustrates heavy chain modifications and Fig. 13B illustrates light chain modifications. N are Asparagines that become aspartic acid and Q are Glutamines that become deamidated. M corresponds to Methionies that become oxidized. The numbers after each amino acid show its position along the amino acid chain from the corresponding protein.Figures 14A- 14B are graphs illustrating percentage of amino acid modifications in Low Skid silk produced in Walpole and Medford using the Skid process with differing process parameters and variable levels. Fig. 14A illustrates heavy chain modifications and Fig. 14B illustrates light chain modifications. N are Asparagines that become aspartic acid and Q are Glutamines that become deamidated. M corresponds to Methionies that become oxidized. The numbers after each amino acid show its position along the amino acid chain from the corresponding protein.Figures 15A- 15D are graphs illustrating percentage of amino acid modifications in Low and Mid silk produced in Skid and Benchtop processes. N are Asparagines that become aspartic acid and Q are Glutamines that become deamidated. M corresponds to Methionies that become oxidized. The numbers after each amino acid show its position along the amino acid chain from the corresponding protein.Figure 16 is an explanation of the method used to calculate percentage ratios of modified amino acids at specific locations along the sequence of each peptide.Figure 17 illustrates an Anion exchange chromatography and size exclusion chromatography scheme of the isolation of Low Skid silk / modified peptide compositions. Low Skid silk / modified polypeptide compositions is composed of a variety of peptide populations, in a wide range of sizes and charge. Using Q- Sepharose anion exchange chromatography as a first step, and HiLoad Superdex 200 size exclusion chromatography as a second purification step, distinct populations of Low Skid silk / modified polypeptide compositions were separated. The Q-Sepharose eluate was loaded onto HiLoad Superdex 200 size exclusion chromatography, which resulted in negatively charged silk compositions / modified peptides fractionated by size.Figures ISA and 18B are chromatograms of the anion exchange chromatography and the following size exclusion chromatography of the eluate (Q-eluate) of Low Skid silk / modified polypeptide compositions. Fig. 18A: Anion exchange chromatography was performed with a Q-Sepharose column (Cytiva). Low- Skid silk / modified peptide compositions were separated to uncharged peptide population (flowthrough - light blue background) and eluted negatively charged silk compositions (eluate - light pink background) by anion exchange chromatography. Light yellow background indicates column wash with 50 mM Tris pH=8.0 before eluting the charged peptide population. Fig. 18B: The negatively charged eluate was loaded onto the Superdex 200 column and was flowed through the column with 50 mM Tris, 200 mM CaC12, pH=8.0. When the UV-280 absorbance started to increase fractions were collected to separate the Low Skid silk / modified peptide compositions by size. The relative elution volume of silk compositions AS77 and AS81 are indicated on the chromatogram.Figures 19A and 19B illustrates the Analytical Size Exclusion Chromatography of Low Skid silk / modified silk compositions and their constituent AS compositions. Fig. 19A. Average molecular weight in kDa of Low Skid silk (LS) and AS77-AS81 are shown. Fig. 19B. Polydispersity (PDI) measurements are shown. The numerical data is presented in Table 7.Figure 20 is a SDS polyacrylamide gel electrophoresis of Low Skid silk / modified polypeptide compositions. Lanes are indicated by fraction number, at the order of elution from the Superdex 200 column, and their respective silk composition: fraction 6 is AS77. fraction 7 is AS78. fraction 8 is AS79. fraction 9 is AS80, and fraction 10 is AS81.Figures 21 A and 21 B are graphs illustrating self-assembly reactions of the of the Low Skid silk / modified peptide compositions. Mid Skid Silk reaction was used as a positive control. Fig. 21A. Illustrate kinetic parameters of gel formation during self- assembly of silk. Self-Assembly parameters of Mid Skid silk: Amax is 0.6780 (Abs). SARF is 8.676, T0.5 is 3.668 h, and the FSAF is 3.08 (Abs / mrn). Fig. 21B. Is a snapshot of a later time point of the same self-assembly assay, 12 days after setting the assay. None of the tested fraction has self-assembled over time.Figures 22A and 22B illustrate the characterization of Low Skid silk compositions by Dynamic Light Scattering. Low skid silk / modified peptide compositions were diluted to a concentration of 1 mg / mL, filtered, and analyzed bythe Zetasizer Pro to estimate the diameter particle size of each silk composition. Fig. 22A. Illustrates intensity diameter particle size distribution measured for silk compositions AS77, AS78, AS79, AS80, and AS81. Fig. 22B. Illustrate correlogram functions of silk compositions AS77, AS78, AS79, AS80, AS81.Figure 23 illustrates size exclusion chromatography scheme of the isolation of Low Skid silk / modified peptide compositions. Low Skid silk / modified polypeptide compositions is composed of a variety of peptide populations, in a wide range of sizes, using HiLoad Superdex 200 size exclusion chromatography, distinct populations of Low Skid silk / modified polypeptide compositions were separated.Figure 24 is a chromatogram of Low Skid silk / modified polypeptide compositions loaded onto a Superdex 200 gel filtration column. Low Skid silk / modified peptide compositions were loaded onto the Superdex 200 column and were flowed through the column with 50 mM Tris, 200 mM CaC12. pH=8.0. When the UV-280 absorbance started to increase fractions were collected to separate the Low Skid silk / modified peptide compositions by size. The relative elution volume of silk compositions AS82, AS86, and AS87 are indicated on the chromatogram.Figures 25A and 25B illustrate Analytical Size Exclusion Chromatography of Low Skid silk / modified silk compositions and their constituent AS compositions. Fig. 25A. Illustrates average molecular weight in kDa of Low Skid silk (LS) and AS82- AS89 are shown. Fig. 25B. Polydispersity (PDI) measurements are shown. The numerical data is presented in Table 9.Figure 26 is an SDS polyacrylamide gel electrophoresis of Low Skid silk / modified polypeptide compositions. Lanes are indicated by fraction number, at the order of elution from the Superdex 200 column, and their respective silk composition: fraction 6 is AS82, fraction 7 is AS83, fraction 8 is AS84, fraction 9 is AS85, and fraction 10 is AS86.Figures 27A and 27B are graphs illustrating self-assembly reactions of the of the Low Skid silk / modified peptide compositions. Mid Skid Silk reaction was used as a positive control. Fig. 27A. Illustrates kinetic parameters of gel formation during self-assembly of silk. Self-Assembly parameters of Mid Skid silk: Amax is 0.6978 (Abs), SARF is 8.591, T0.5 is 3.361 h. and the FSAF is 3.46 (Abs / min). Fig. 27B. Is a snapshot of a later time point of the same self-assembly assay, 18 days after settingthe assay. AS87, AS88, and AS89 demonstrate gel formation at this time point, that was already observed five days post assay (LS, Low Skid silk; MS, Mid Skid silk).Figures 28A- 28C are graphs showing characterization of Low Skid silk compositions by Dynamic Light Scattering. Low skid silk / modified peptide compositions were diluted to a concentration of 1 mg / mL, filtered, and analyzed by the Zetasizer Pro to estimate particle size of each silk composition. Fig. 28A. Shows intensity particle size distribution measured for silk compositions AS82, AS83. AS84, AS85, AS86, AS87, AS88, and AS89. Fig. 28B. Shows intensity particle size distribution measured for silk compositions AS82, Low Skid silk / modified peptide compositions (LS), and Mid Skid silk / modified peptide compositions (MS). Fig. 28C. Shows correlogram functions of silk compositions AS82, AS83, AS84, AS85, AS86. AS87, AS88. AS89, Low Skid silk / modified peptide compositions (LS), Mid Skid silk / modified peptide compositions (MS).Figure 29 illustrates anion exchange chromatography (Q), hydrophobic interaction chromatography (HIC), and size exclusion chromatography (SEC) scheme of the isolation of Low Skid silk / modified peptide compositions. Low Skid silk / modified polypeptide compositions is composed of a variety of peptide populations, in a wide range of sizes and charge. Using Q-Sepharose anion exchange chromatography as a first step, Buty l ImpRes Hydrophobic interactions resin as a second step, and HiLoad Superdex 200 size exclusion chromatography as a third purification step, distinct populations of Low Skid silk / modified polypeptide compositions were isolated. The Q-Sepharose eluate was loaded onto a Butyl ImpRes (HIC) column, and the HIC-eluate was loaded onto a HiLoad Superdex 200 size exclusion chromatography, which resulted in fractionation of negatively charged silk compositions / modified peptides with hydrophobicity characteristics fractionated bysize. The Q-Sepharose eluate contained negatively charged peptides in all sizes. Resolving these peptides by Butyl ImpRes column resulted in elution of high- molecular-weight, negatively charged, somewhat hydrophobic silk compositions / modified peptides. The smaller negatively charged peptides were washed as flowthrough and did not bind the Butyl ImpRes column. The Q- HlC(elution) was loaded into Superdex 200 and was separated by size.Figures 30A- 30E are chromatograms of anion exchange chromatography, hydrophobic interactions chromatography, and the following size exclusion chromatography of Low Skid silk / modified polypeptide compositions. Fig.30A. illustrates anion exchange chromatography was performed with a Q-Sepharose column. Low Skid silk / modified peptide compositions were separated to uncharged peptide population (flowthrough - light blue background) and eluted negatively- charged silk compositions (eluate - light pink background) by anion exchange chromatography. Light yellow background indicates column wash with 50 mM Tris pH=8.0 before eluting the charged peptide population. Fig.30B. illustrates the negatively charged eluate (Q-elution) was loaded onto a Butyl ImpRes column, in the presence of 300 mM ammonium sulfate [(NH4)2SO4], to expose hydrophobic domains of the silk peptides, which allows binding to the column. The highly charged peptide population did not bind the column (flowthrough), highlighted in light blue. The column was washed until OD280 was reduced to -100 units (light yellow). Then, the bound silk peptides (Q-HIC(elution)) were eluted by using 50 mM Tris, pH=8.0 without ammonium sulfate (light pink). Fig.30C. illustrates the Q-HlC(elution) was further fractionated by size exclusion chromatography (SEC), using the gel filtration column Superdex 200. The Q-HIC(elution) fraction was flowed through the column with 50 mM Tris, 200 mM CaC12, pH=8.0. When the UV-280 absorbance started to increase fractions were collected to separate the Low Skid silk / modified peptide compositions by size. The relative elution volume of silk compositions AS90 and AS94 are indicated on the chromatogram. Fig.30D. the Q-HIC(flowthrough) fraction was further fractionated by SEC. using the Superdex 200 column, at the same procedure as in (VC). The relative elution volume of silk compositions AS95 and AS 100 are indicated on the chromatogram. Fig.30E. illustrates the superimposition of chromatograms (VC) and (VD). the Q-HIC(elution) fraction has a higher-molecular- weight range compared to the Q-HIC( flow through) fractions, which elutes later in SEC, and has lower-molecular-weight range.Figures 31A- 31B are graphs showing analytical Size Exclusion Chromatography of Low Skid silk / modified silk compositions and their constituent AS compositions. Fig.31A. Average molecular weight in kDa of Low- Skid silk (LS) and AS90-AS100 are shown. Fig.31B. Poly dispersity- (PDI) measurements are shown. The numerical data is presented in Table 11.Figures 32A- 32B are SDS polyacrylamide gel electrophoresis of Low Skid silk / modified polypeptide compositions. Fig. 32A. Q-HIC(elution) SEC fractions. Fig. 32B. Q-HIC(flowthrough) SEC fractions. Lanes are indicated by fraction number, at the order of elution from the Superdex 200 column, and their respective silk composition: in Fig. 32A, fraction 6 is AS90, fraction 7 is AS91, fraction 8 is AS92, fraction 9 is AS93, and fraction 10 is AS94. In Fig. 32B, fraction 8 is AS95. fraction 9 is AS96. fraction 10 is AS97, fraction 11 is AS98, fraction 12 is AS 99. and fraction 13 is AS 100.Figure 33 illustrates self-assembly reactions of the of the Low Skid silk / modified peptide compositions. Mid Skid Silk reaction was used as a positive control, kinetic parameters of gel formation during self-assembly of silk. Q- HlC(elution) is the elution fraction that was eluted from the Butyl ImpRes column, prior to SEC purification; LS, Low Skid silk; MS, Mid Skid silk. Self-Assembly parameters of Mid Skid silk: Amax is 0.6974 (Abs), SARF is 8.661, T0.5 is 3.834 h, and the FSAF is 3.03 (Abs / min).Figures 34A- 34F illustrate the characterization of Low Skid silk compositions by Dynamic Light Scattering. Low and Mid skid silk / modified peptide compositions were diluted to a concentration of 1 mg / mL, filtered, and analyzed by the Zetasizer Pro to estimate the diameter particle size of each silk composition. Fig. 34A. Intensity diameter particle size distribution measured for silk compositions AS90, Q-HIC(elution) fraction (prior to fractionation by SEC), Low Skid silk (LS), and Mid Skid silk (MS). Fig. 34B. Correlogram functions of silk compositions presented in (34A). Fig. 34C. Intensity diameter particle size distribution measured for silk compositions AS90-AS94, derived from Q-HIC(elution)-SEC fractionation process. Fig. 34D. Correlogram functions of silk compositions presented in (34C). Fig. 34E. Intensity diameter particle size distribution measured for silk compositions AS95-AS100, derived from Q-EIIC(flowthrough)-SEC fractionation process. Fig. 34F. Correlogram functions of silk compositions presented in (34E).Figure 35. illustrates size exclusion chromatography scheme of the isolation of Mid Skid silk / modified peptide compositions. Mid Skid silk / modified polypeptide compositions is composed of a variety of peptide populations, in a wide range of sizes, using HiLoad Superdex 200 size exclusion chromatography, distinctpopulations of Mid Skid silk / modified polypeptide compositions were able to be separated.Figure 36. Is a chromatogram of Mid Skid silk / modified polypeptide compositions loaded onto a Superdex 200 gel filtration column. Mid Skid silk / modified peptide compositions were loaded onto the Superdex 200 column and were flowed through the column with 50 rnM Tris, 200 mM CaC12, pH=8.0. When the UV-280 absorbance started to increase fractions were collected to separate the Mid Skid silk / modified peptide compositions by size. The relative elution volume of silk compositions AS 107 and AS1 11 are indicated on the chromatogram.Figures 37A- 37B. Illustrates analytical Size Exclusion Chromatography of Mid Skid silk / modified silk compositions and their constituent AS compositions. Fig. 37A. Average molecular weight in kDa of Mid Skid silk (MS) and AS 106- AS 111 are shown. Fig. 37B. Poly dispersity (PDI) measurements are shown. The numerical data is presented in Table 14.Figure 38. Is a SDS polyacrylamide gel electrophoresis of Mid Skid silk / modified polypeptide compositions. Lanes are indicated by fraction number, at the order of elution from the Superdex 200 column, and their respective silk composition: fraction 6 is AS107, fraction 7 is AS108, fraction 8 is AS109. fraction 9 is AS 110, and fraction 10 is AS 11 1.Figure 39. Illustrates self-assembly reactions of the of the Mid Skid silk / modified peptide compositions. Kinetic parameters of gel formation during self- assembly of silk. Dashed red lines show how the self-assembly parameters Amax, SARF, and TO.5 were calculated for unfractionated Mid Skid silk (MS). These numerical calculated parameters of silk compositions AS106-AS111 can be found in Table 16. Low Skid silk (LS) was used as a negative control. LS, Low Skid silk; MS, Mid Skid silk.Figures 40A-40B. Illustrates characterization of Mid Skid silk compositions by Dynamic Light Scattering. Mid skid silk / modified peptide compositions were diluted to a concentration of 1 mg / mL, filtered, and analyzed by the Zetasizer Pro to estimate particle size of each silk composition. Fig. 40A. Intensity particle size distribution measured for silk compositions AS 106, AS 107, AS 108, AS 109, AS 110,AS111, and Mid Skid (MS). Fig. 50B. Correlation functions of silk compositions presented in (40A).Figure 41. Illustrates anion exchange chromatography and size exclusion chromatography scheme of the isolation of Mid Skid silk / modified peptide compositions. Mid Skid silk / modified polypeptide compositions is composed of a variety of peptide populations, in a wide range of sizes and charge. Using Q- Sepharose anion exchange chromatography as a first step, and HiLoad Superdex 200 size exclusion chromatography as a second purification step, distinct populations of Mid Skid silk / modified polypeptide compositions were separated. The Q-Sepharose eluate was loaded onto HiLoad Superdex 200 size exclusion chromatography, which resulted in negatively charged silk compositions / modified peptides fractionated by size.Figures 42A-42B. Are chromatograms of the anion exchange chromatography and the following size exclusion chromatography of the eluate (Q-eluate) of Mid Skid silk / modified polypeptide compositions. Fig. 42A. Anion exchange chromatography was performed with a Q-Sepharose column (Cytiva). Mid Skid silk / modified peptide compositions were separated to uncharged peptide population (flowthrough - light blue background) and eluted negatively charged silk compositions (eluate - light pink background) by anion exchange chromatography. Light yellow background indicates column w ash with 50 mM Tris pH=8.0 before eluting the charged peptide population. Fig. 42B. The negatively charged eluate (Q-elution) was loaded onto the Superdex 200 column and was flowed through the column with 50 mM Tris, 200 mM CaC12, pH=8.0. When the UV-280 absorbance started to increase fractions w ere collected to separate the Mid Skid silk / modified peptide compositions by size. The relative elution volume of silk compositions AS 101 and AS 105 are indicated on the chromatogram.Figures 43A-43B. Illustrate analytical Size Exclusion Chromatography of Mid Skid silk / modified silk compositions and their constituent AS compositions. Fig. 43A. Average molecular w eight in kDa of Mid Skid silk (MS) and AS101-AS105 are shown. Fig. 43B. Poly dispersity (PDI) measurements are shown. The numerical data is presented in Table 16.Figure 44A. Is a SDS polyacrylamide gel electrophoresis of Mid Skid silk / modified polypeptide compositions. Lanes are indicated by fraction number, at the order of elution from the Superdex 200 column, and their respective silkcomposition: fraction 6 is AS101, fraction 7 is AS102, fraction 8 is AS103, fraction 9 is AS104, and fraction 10 is AS 105.Figure 44B illustrates self-assembly reactions of the of the Mid Skid silk / modified peptide compositions. Low Skid Silk reaction was used as a negative control. Kinetic parameters of gel formation during self-assembly of silk are shown. Red dotted lines are shown to clarify the calculations of Amax, SARF (Self-Assembly Rate Factor), and TO.5 parameters in Table 17.Figures 45A-45C. Are graphs illustrating characterization of Mid Skid silk compositions by Dynamic Light Scattering. Mid skid silk / modified peptide compositions were diluted to a concentration of 1 mg / mL. filtered, and analyzed by the Zetasizer Pro (Malvern) to estimate the diameter particle size of each silk composition. Fig. 45A. Intensity diameter particle size distribution by intensity measured for silk compositions AS101, AS102, AS103, AS104, and AS105. Fig. 45B. Intensity diameter particle size distribution by intensity measured for silk compositions AS101, AS105, and Mid Skid silk (MS), to emphasize the size difference between AS 101 and AS 105. Fig. 45C. Correlogram functions of silk compositions AS101, AS102, AS103, AS104, AS105, and Mid Skid silk (MS).Figure 46. is an illustration of the values for three molar mass moments (Mn, M w. and Mz) as it relates to molar mass and the number of molecules at each molar mass. This example is applicable to a polydisperse sample; for a monodisperse sample, Mn = Mw = Mz.Figures 47A- 47B are analytical SEC-MALS of Low, Mid and High Molecular Weight Silk. Fig. 47A. Weight Average Molecular Weight in kDa of Low, Mid, and High Molecular Weight Silk. Fig. 47B. Poly dispersity Index (PDI) measurements of Low, Mid, and High Molecular Weight Silk are shown.Figures 48A- 48B. Are analytical SEC-MALS of Low, Mid and High Molecular Weight Silk organized by Silk Type produced by different process parameters and variable levels. Individual data points are shown and the mean is represented by the heigh of the box. The bars encompass one standard deviation. Fig. 48A. Weight- Av erage Molecular Weight Ranges for Low, Mid, and High Molecular Weight Silk. Fig. 48B. PDI Ranges for Low, Mid, and High Molecular Weight Silk.Figures 49A- 49B are nalytical SEC-MALS of Low Skid silk / modified silk compositions and the constituent AS compositions as separated by Q-SEC (Q-eluent). Fig. 49A. Average molecular weight in kDa of Low Skid silk (LS) and AS77-AS81are shown. Fig. 49B. Poly dispersity (PDI) measurements are shown. The numerical data is presented in Table 24.Figures 50A- 50B are analytical SEC-MALS of Low Skid silk / modified silk compositions and the constituent AS compositions as separated by SEC. Fig. 50A. Average molecular weight in kDa of Low Skid silk (LS) and AS82-AS89 are shown. Fig. 50B. Poly dispersity (PDI) measurements are shown. The numerical data is presented in Table 25.Figures 51 A- 51B are analytical SEC-MALS of Low Skid silk / modified silk compositions and the constituent AS compositions as separated by Q-HIC-SEC (Q- HIC-Eluent). Fig. 51A. Average molecular weight in kDa of Low Skid silk (LS) and AS90-AS94 are shown. Fig. 51B. Polydispersity (PDI) measurements are shown. The numerical data is presented in Table 26.Figures 52A- 52B are analytical SEC-MALS of Low Skid silk / modified silk compositions and the constituent AS compositions as separated by Q-HIC-SEC (Q- HIC-Flowthrough). Fig. 52A. Average molecular weight in kDa of Low Skid silk (LS) and AS95-AS100 are shown. Fig. 52B. Poly dispersity (PDI) measurements are shown. The numerical data is presented in Table 26.Figures 53A- 53B are analytical SEC-MALS of Mid Skid silk / modified silk compositions and the constituent AS compositions as separated by Q— SEC (Q-flow through). Fig. 53A. Average molecular weight in kDa of Mid Skid silk (MS) and AS101-AS105 are shown. Fig. 53B. Poly dispersity (PDI) measurements are shown. The numerical data is presented in Table 27.Figures 54A- 54B are analytical SEC-MALS of Mid Skid silk / modified silk compositions and the constituent AS compositions as separated by SEC. Fig. 54A. Average molecular weight in kDa of Mid Skid silk (MS) and AS106-AS111 are shown. Fig. 54B. Poly dispersity (PDI) measurements are shown. The numerical data is presented in Table 28.Figure 55 is a chart illustrating mean TEWL values for barrier redux emulsion.Figure 56 is a chart illustrating the effect of the barrier redux emulsion on w rinkles and photoaging.Figure 57 is a chart illustrating the effect of the barrier redux emulsion on hyperpigmentation.Figure 58 is a chart illustrating the effect of the barrier redux emulsion on wrinkles.Figure 59 is a chart illustrating the effect of the barrier redux emulsion on crow’s feet wrinkles.Figure 60 is a chart illustrating the effect of the barrier redux emulsion on crow's feet lines.Figure 61 is a chart illustrating effect of the barrier redux emulsion on marionette area wrinkles.Figure 62 is a chart illustrating the effect of the barrier redux emulsion on skin roughness.Figure 63 is a chart illustrating the effect of the barrier redux emulsion on global wrinkles.Figure 64 is a chart illustrating the effect of the barrier redux emulsion on global fine lines.Figure 65 is a chart illustrating the effect of the barrier redux emulsion on the appearance of medium, deep lines and wrinkles.Figure 66 is a chart illustrating the effect of the barrier redux emulsion on skin tone evenness.Figure 67 is a chart illustrating the effect of the barrier redux emulsion on skin texture.Figure 68 is a chart illustrating the effect of the barrier redux emulsion on skin scaling.Figures 69A - 69C show the sequence listing for fibroin heavy chain.Figure 70 shows the sequence listing for fibroin light chain.Figure 71 shows the sequence listing for fibrohexamerin.Figure 72 is a flow chart showing various embodiments for producing silk fibroin protein fragments (SPFs) of the present disclosure.Figure 73 is a flow chart showing various parameters that can be modified during the process of producing a silk protein fragment solution of the present disclosure during the extraction and the dissolution steps.Figure 74 is a graph illustrating the improvement in Investigator Global Assessment (IGA) after using the Body Wash.Figure 75 is a graph illustrating the improvement in redness after using the Body Wash.Figure 76 is a graph illustrating the improvement in eczema count after using the Body Wash.Figure 77 is a graph illustrating the improvement in individual comeometer after using the Body Wash.Figure 78 is a graph illustrating the improvement in individual TEWL after using the Body Wash.Figure 79 is a graph illustrating responses of the Self-perception Questionnaires after using the Body Wash.Figure 80 is a graph illustrating responses of the Self-perception Questionnaires after using the Body Wash.Figure 81 illustrates three chromatography principles of silk fractionalization.Figure 82 illustrates the size exclusion chromatography of silk fractionalization.Figure 83 illustrates the anion exchange chromatography followed by size exclusion chromatography of silk fractionalization.Figure 84 illustrates the anion exchange chromatography followed by hydrophobic interactions chromatography and size exclusion chromatography.Figure 85 is a chart summarizing the three pipelines of silk fractionalization.Figure 86 is the characterizations methods of silk fractionalization.Figure 87 illustrates size exclusion chromatography of Low Skid silk.Figure 88 illustrates an example of silk composition characterization: Size exclusion chromatography of Low Skid silk.Figure 89 illustrates an example of silk composition characterization: Size exclusion chromatography of Low Skid silk Dynamic tight scattering.Figure 90 is a chart including Z-averages of Low Skid silk / modified polypeptide composition.Figure 91 illustrates molecular weight and Polydispersity determination by HPLC.Figure 92 illustrates Silk fractionation Size exclusion chromatography of Low Skid silk. Lower-molecular- weight fractions self-assemble slowly over time.Figure 93 is a chart including assays for characterizing silk fractions.Figure 94 is a comparison of Molecular weight and Poly dispersity determination by two methods.Figure 95 is a photograph of powdered emulsifier formulation with 1:2 ratio of 10% liquid silk and xanthan gum powderFigure 96 is a graph of viscosity of silk emulsions with increasing oil concentration.Figure 97 is a graph of shear thinning viscosity of alpha 720 silk emulsions with varying oil concentrations.Figure 98 is a comparison of shear thinning viscosity of mid skid silk emulsions compared to other formulations.Figure 99 is a graph of storage modulus of formulations of alpha 720 silk with xanthan gum and varying oil concentrations.Figure 100 is a graph of storage modulus of formulations of alpha 720 silk with sclerotium gum and varying oil concentrations.Figure 101 is a graph of storage modulus of formulations of alpha 113 silk with xanthan gum and varying oil concentrations.Figure 102 is a graph of storage modulus of formulations of alpha 113 silk with sclerotium gum and varying oil concentrations.Figure 103 is a graph of storage modulus and loss modulus of freshly reconstituted and 2 week old formulations of alpha 720 silk and xanthan gum.Figure 104 is an illustration of non-occlusive Activated Silk 33B-α.Figure 105 is an illustration of Activated Silk 33B-α working to firm and regulate claudin-1 production.Figure 106 is an illustration of claudin-1 up-regulation.Figure 107 is an image of untreated skin. Claudin-1 (orange stain) and cell nuclei (blue stain).Figures 108A and 108B are images of insulted skin 24 hours after control and test treatments. Claudin-1 (orange stain) and cell nuclei (blue stain). 108A is not Activated Silk 33B-α. 108B is Activated Silk 33B-α.Figure 109 is a graph illustrating percent change in transepidermal water loss (TEWL). P-Value: <0.05.Figure 110 is a graph illustrating reduction in redness. P-Value: <0.05.Figure 111 is a graph illustrating reduction in wrinkles. P-Value: <0.05.Figure 112 is a graph illustrating clinical responses / bioactive peptide activity validations.Figure 113 is an image of a skin model tissue (red) and Activated Silk™ 33B- α 2% (grey). Fig. 113 is Post wash: Activated Silk™ 33B-α 2% with capryl glucoside. Activated silk™ 33B-α 2%: GREY STAIN.Figure 114are images on a skin model tissue. Figure 114 is Pre-wash: without Activated Silk™ 33B-α.Figure 115 is a graph illustrating percent change in transepidermal water loss (tewl).Figure 116 is a graph illustrating appearance of percent change in redness.Figure 117 is a graph illustrating reduction in visible symptoms associated with eczema-prone skin. Clinical results show pow erful evidence that the use of the Activated Silk™ 33B- ingredient visibly reduces symptoms associated with eczema- prone skin.Figure 118 is a photograph of Activated Silk™ 33B-α in pellet form.Figure 119 are graphs illustrating total collagen deposition.Figure 120A and 120B are microscopic views of collagen levels. Figure 120A is the control and Figure 120B is Activated Silk™ 27P-α**. , **Magnification level 10X, Cells stained with Sirius Red.Figure 121 are photographs showing improvements in skin health.Figure 122 is a graph illustrating wrinkle reduction for Activated silk™ 27P-α vs. placebo control.Figure 123 is a graph illustrating improvement in skin firmness for Activated silk™ 27P-α vs. placebo control.Figure 124 is a graph illustrating improvement in skin redness for Activated silk™ 27P-α vs. placebo control.Figure 125 is a graph illustrating clinical responses / bioactive peptide activity validations.Figure 126 are graphs illustrating late-state keratinocyte differentiation. Activated silk 8A-α Penetrates epidermis and promotes differentiation to move spot pigmentation out of skin, as evidenced by an up-regulation in involucrin - a protein that signals the later stages of keratinocyte differentiation.Figure 127 are graphs illustrating Activated silk 8A-α effect on skin brightness and skin hyperpigmentation.Figure 128 are images illustrating Activated silk 8A-α effect on skin brightness and skin hyperpigmentation. Measure of the ratio of red light vs. green light reflected from skin. The higher the value on a color scale from 1 (black) to 28 (white) indicates the total level of skin tone above normal levels.Figure 129 are images illustrating Activated silk 8A-α effect on skin brightness and skin hyperpigmentation.Figure 130 are images illustrating Activated silk 8A-α effect on skin brightness and skin hyperpigmentation.Figure 131 are images illustrating Activated silk 8A-α effect on skin brightness and skin hyperpigmentation.Figure 132 is a graph illustrating regeneration of damaged skin with Keratinocyte Differentiation Factor (KDF). Activated Silk™ KDF regenerates and hydrates the outer layers of the epidermis - fighting hyperpigmentation and the oxidative effects of environmental pollution at lower doses and offering non-steroidal relief from symptoms associated with eczema prone skin at higher doses. KDF Penetrates epidermis and promotes keratinocyte differentiation to regenerate the epidermis via the up-regulation of differentiation proteins keratin 1 and 10. Hydrates via the production of filaggrin. a natural skin hydration protein. Creates skin barrier integrity via the production of claudin and occludin.Figure 133 is a photograph showing skin on day 0, before KDF treatment.Figure 134 is a photograph showing an improvement in skin health on day 28 after KDF treatment.Figure 135 are images showing KDF increases Keratin 1 expression in skin tissue, resulting in differentiation of keratinocytes.Figure 136A- 136C illustrates three different peptide matrices, Fig. 136A:X01, Fig. 136B: X02 (for skin delivery), and Fig. 136C: X03 (for skin and cell delivery).Figure 137 are images showing emulsified X01.Figure 138 illustrates a hydrogel (pink) emulsified 40% oil (not stained) and actively bound to the surface of skin which has been clinically proven to increase hydration long after product application and washing. X01 (pink) was digitally “lifted” from its bound surface.Figure 139 is a graph illustrating the peptide Matrix hydrogel (1% w / v) viscosity as a function of with increasing oil (met) concentration.Figure 140 is a graph illustrating Viscosity as a function of Peptide Matrix hydrogel concentration.Figure 141 is a graph illustrating structural stability from a network effect formed by Activated Silk™ Peptide Matrix hydrogels.Figure 142 are images depicting X01 hydrogel, after emulsification with oil.Figures 143A-143D illustrated skin penetration (control) Image (A) showing planar view mid- thickness of the epidermis, (B) showing topical view, and (C) Skin penetration (24hrs) hydrogel with emulsified oil (sphere above, labeled pink on right) penetrates epidermis following topical application.(D) shows a size scale.Figure 144 are images showing X03 Peptide Matrix Hydrogels. Delivery of emulsified oil active (pink) into keratinocytes.Figure 145 is a graph illustrating the spreadability (In comparison to benchmark-No Glycerin).Figure 146 is a graph illustrating spreadability (Shear thinning profile; 1% MTR-01). The viscosity profiles above show that with increasing oil concentrations, the emulsions are significantly more viscous at rest storage (at very low shear rates between 0.001s-1and 0.1s-1). However, on application (> 100s-1), they all have similar viscosities and feel relatively the same.Figure 147 is a graph illustrating textures for different product formats. Fig. 147 shows the viscosity of emulsions made with MTR-1 and MTR-2.Figure 148 is a graph illustrating structural strength (stability) in emulsions made with 1% emulsifier. As shown in the plot, the emulsion made with MTR emulsifiers have a much higher storage modulus and yield stress than the emulsion with Olivem-1000. Emulsions also passed freeze-thaw testing (3 cycles; -5C to 30C) and centrifugations (4000 rpm, 20mins). Emulsions confirmed stable over a pH range from 3 to 7 and at salt concentrations up to 2%.Figure 149 are representative images of emulsifier silk binding to biosurface. Emulsion on hair: Silk is red, oil is green, blue is hair autofluorescence.Figure 150 are representative images of emulsifier silk binding to biosurface, but contrast is increased, showing the coating.Figure 151 are representative images showing silk emulsion coating a monolayer of fixed cells (green is WGA-cell bodies, magenta is mid silk).Figures 152A- 152B are 3D Renderings showing silk coating corneal envelope. Fig. 152A is the mock rendering. Fig. 152B is 10 mg / ml Silk.Figures 153A- 153E are images showing emulsion visualization with labeled silk. Fig. 153A shows emulsions formulated with Alexa-594 labeled silk (mid and low) were visualized at 1 :3 dilution in water using confocal microscopy and silk deposits on vesicle surface (Mid Skid). Fig. 153B is an image showing Mid Skid (Maximum intensity projection). Fig. 153C includes a scale bar. Fig. 153D shows Mid Skid (single z slice). Fig. 153E shows Low Skid (Maximum Intensity Projection).Figure 154 is an image showing formulations tested in the centrifuge stability (5000 rpm. 20mins). L-R: (1) Emulsion made with 1% LMR-2 stable after centrifugation (2) Emulsion made with 1% LMR-1 stable after centrifugation (3) Emulsion made with 1% Olivem 1000 MB separated after centrifugation.Figure 155 is an image showing formulations tested in the freeze-thaw stability (3 cycles -5C to 30C). Top to bottom: (1) Emulsion made with 0.5% - 1.25% 720X stable after FT cycling (2) Emulsion made with 0.5% - 1.25% 113X stable after FT cycling (3)Emulsion made with 0.5%, 1% , 2.5% and 5% Olivem 1000 MB separated after FT cycling.Figure 156 is an image showing formulations tested in the centrifuge stability (5000 rpm. 20mins). L-R: (1) Emulsion made with 3% Olivem and 30% oil unstable after centrifugation.Figure 157 is an graph showing textures for different applications. Emulsions of 20% olive oil in water would require about 2.1% Olivem 1000 MB to achieve same viscosity as 1% low silk emulsifier and about 3.25% to achieve similar viscosity as mid emulsifier. With Olivem 1000 the maximum oil load is 20%, while with silk- xanthan emulsifier stable emulsions of up to 50% oil in water could be achieved. This is particularly interesting for food emulsions which typically contain higher oil concentrations than cosmetic applications.Figure 158 is a graph showing that a drastic increase in viscosities can also be achieved with the silk emulsifier by varying oil concentrations. (2) Emulsion made with 3% Olivem and 30% oil unstable after centrifugation (3) Emulsion made with 1% Olivem 1000 MB separated after centrifugation.Figure 159 is a graph showing shear thinning profile on application. Shear thinning rheology ideal to show that the emulsions can be spread easily onapplication. The steady state viscosity profiles show that with increasing oil concentrations, the emulsions are significantly more viscous on storage (btw 0.001s1and 0.1s-1). However, on application (btw 100s-1and 10000s-1), they all have similar viscosities and feel relatively the same.Figures 160A- 160D are graphs showing evaluation of yield stress in emulsions of 20% olive oil in water using 1% emulsifier. The minimum amount of stress required to break down the internal structure of a complex fluid like an emulsion and allow it to flow. As shown in the plot, the emulsion made with LMR emulsifiers have a much higher storage modulus and yield stress than emulsion with Olivem-1000.Figure 161 is a graph showing the effect of pH on viscosity of emulsions. Changes in the pH have no effect on the stability of the silk-xanthan emulsions up to pH 7.5. (centrifuge stability and FT Test all passed) Beyond 7.5, color change is observed in the emulsions which predicts chemical instability on the emulsions. Changes in the pH also affect the viscosity of the emulsions as shown in the graph.Figure 162 is a graph showing the effect of salt on viscosity of emulsions. Changes in NaCl concentration had no effect on the stability of the silk-xanthan emulsions up to 2% (FT and centrifuge stability ). Changes in the NaCl concentration also had minimal effects on the viscosity of the emulsions as shown in the plot.Figure 163 is a graph showing the results of the thermal cycle stability test.Figure 164 is a graph showing relative structural change in emulsions after induced accelerated thermal instability . The sample that shows a smaller value for the relative structural change at the end of the thermal cycles has the higher thermal stability. From the results, the emulsion made with the lyo noodles seems more structurally resilient to thermal instability compare to the emulsion made with the powder blend of silk and xanthan gum.Figure 165 is a bar graph show ing viscosity profiles of various silk + gum emulsifiers.Figures 166A- 166B are images showing emulsions size detected by optical microscope. Fig. 166A. Emulsion made with Olivem 1000 (13.93 um), and Fig. 166B. emulsion made with silk emulsifier (34.72 um).Figures 167A- 167B are images showing oil droplets movement checked by Optical microscope. Fig. 167A. Olivem 1000MB (1%)- 40X, and Fig. 167B. Silk emulsifier ( l %-low-20% oil)- 10X. Optical microscope videos show the oil dropletsin the Olivem emulsions moving around while the silk emulsions remain static, which indicates that silk emulsion is more stable than the Olivem emulsionFigures 168A- 168B are images showing oil droplets movement checked by Optical microscope. Emulsions size detected by optical microscope images of Fig. 168A. Emulsion made at 8000 rpm for 2.5 min (52.58 um), and Fig. 168B, emulsion made at 8000 rpm for 15 min(21.59 um). Both the emulsion droplets size and the size poly dispersity decreased with a longer stirring time.Figures 169A- 169B are images showing polarized microscopy images of Olivem emulsions. Minimal Lamellar structure was found in emulsion made with Olivem. No Lamellar structure was found in emulsions made with silk emulsifier.Figures 170A- 170B are Cryo SEM images of emulsions made with low skid silk (Ag-tagged silk, 1% emulsifier. 20% of oil, no glycerin).Figures 171A- 171B are Cryo SEM images of emulsions made with mid skid silk (Ag-tagged silk, 1% emulsifier. 20% of oil, no glycerin). More silk network / scaffold were found in the emulsions made with mid silk thant low silk. It might be due to the mid skid silk has 1. Longer chain. 2. Less charges, 3. More hydrophobic. More network / scaffold also means more stable.Figures 172A- 172B are Cry o SEM images of emulsions made with Olivem 1000 (no silk, 1% emulsifier. 20% of oil, no glycerin).Figures 173A- 173B are Cryo SEM images of emulsions made with xanthan gum (no silk, 1% emulsifier. 20% of oil, 5% glycerin).Figures 174A- 174B are Cry o SEM images of emulsions made with xanthan gum (no silk, 1% emulsifier. 20% of oil, 5% glycerin).Figure 175 is an image showing Mid Skid -594 with green fluorescent Medium-chain triglycerides (MCT-Green). The lipids are on the inside and the silk is on the outside (MCT-Green: 18: 1-18: 1-C11 TopFluor).Figure 176 is an image showing fluorescence dye tagged silk deposits on vesicle surface (Mid Skid).Figure 177 is an image showing a Mid Skid Emulsion.Figure 178 is an image showing a Low Skid (Maximum Intensity Projection).Figure 179 is a Silk matrix structure with branched filaments 3D rendering.Figures 180A- 180C are images showing displacement of oil by silk filaments (single slice).Figure 181 is an image showing an undiluted mid emulsion - 2D projection.Figure 182 is an image showing an undiluted low emulsion - 2D projection.Figures 183A- 183C are images showing silk emulsions. Fig. 183A shows Low Skid, Fig. 183B shows Mid Skid, and Fig. 183C shows the scale.Figure 184 is an image showing wheat germ agglutinin (“WGA”) -stained keratinocyte sheet (example).Figures 185A- 185B are images showing wheat germ agglutinin. Large protein that labels the surface of cells but cannot get in. FIG. 185A shows the top of cell cluster, Green-WGA labels surface of cells. FIG. 185B shows the middle of the cell cluster, Green-WGA cannot get in through.Figure 186 are images showing WGA 48 hours treatment followed by 4 washes.Figures 187A and 187B depict a schematic overview of the experimental setup. FIG 187A: Hairs were disinfected with 90% ethanol and washed with water. Then, hairs were incubated with or without biotinylated silk. The hairs were washed to discard unbound biotinylated silk, and then the hairs were heated to 95°C, for 10 min, in the presence of SDS and B-mercaptoethanol (BME). The supernatant was collected and contained extracted biotinylated silk and hair proteins. The extract was loaded onto an SDS-PAGE gel, and proteins were transferred to a PVDF membrane. Biotinylated silk was detected by interacting the membrane with Streptavidin conjugated to horseradish peroxidase (Streptavidin-HRP). and the membrane was exposed. FIG. 187B: Hairs were disinfected and washed with 90% ethanol and washed with water. Then, hairs were incubated with or without fluorescently labeled silk / products that contain fluorescently labeled silk. The hairs were washed to discard unbound fluorescently labeled silk and were imaged with a fluorescent microscope.Figures 188A and 188B show pulldown of bound silk on hairs. FIG. 188A shows SDS-PAGE of extracted hair proteins and silk: Hairs were disinfected with 90% ethanol and washed with water. Then, hairs w ere incubated with varying concentrations of silk (60, 30, 10, 5 mg / mL, or just PBSX1). The hairs were washed to discard unbound silk, and then heated to 95 C, for 10 min, in the presence of SDS and B-mercaptoethanol (BME). The supernatant w as collected and contained extracted silk and hair proteins. The extract was loaded onto an SDS-PAGE gel to visualize the difference between samples. FIG. 188B shows a Western Blot to detect pulldown of biotinylated silk on hairs. Hairs were disinfected with 90% ethanol and washed with water. Then, hairs were incubated with or without 10 mg / mL Low Skid and Mid Skidbiotinylated silk. Additionally, hair samples were incubated with 10 mg / mL unlabeled Low Skid and Mid Skid silk. The hairs were washed to discard unbound biotinylated silk, and then the hairs were heated to 95 C, for 10 min, in the presence of SDS and β- mercaptoethanol (BME). The supernatant was collected and contained extracted biotinylated silk and hair proteins. The extract was diluted 1 : 10, and loaded onto an SDS-PAGE gel, and transferred to a PVDF membrane. Biotinylated silk was detected by interacting the membrane with Streptavidin conjugated to horseradish peroxidase (Streptavidin-HRP), and the membrane was exposed. LS, Low Skid silk; MS, Mid Skid silk; LS-B, biotinylated Low Skid silk; MS-B, biotinylated Mid Skid silk.Figure 189 depicts images of hairs post-incubation with Low SkidA594, Mid SkidA594 silk peptide compositions or control unlabeled silk. Both Low skid and Mid skid silk polypeptide compositions at various concentrations left residual labeled silk polypeptides on hair when visualized by confocal microscopy (red, left panels). Hair structure was visualized by autofluorescence (cyan) after 405 nm excitation (merged images on right panels). Note the lack of fluorescence seen after incubation with 10 mg / ml unlabeled Low Skid silk, indicating that fluorescent signal is specific to labeled silk polypeptides. Images are shown as maximum Z-proj ections for each channel.Figure 190 depicts images of hairs post-incubation with various formulas containing A594-labeled silk. Residual silk is detected on hair when applied in both shampoo and conditioner formulas followed by washing. Application of emulsions containing either Low skid or Mid Skid as well as Topfluor-TG, a green fluorescent triglyceride, leads to both oil phase and silk peptide retention on hair.Figure 191 illustrates changes in the pH have no effect on the stability of the silk-xanthan emulsions up to pH 7.5. (centrifuge stability and FT Test all passed) Beyond 7.5, color change is observed in the emulsions which predicts chemical instability in the emulsions, changes in the pH also affect the viscosity of the emulsions as shown in the plot.Figure 192 illustrates changes in the NaCl concentration had no effect on the stability of the silk-xanthan emulsions up to 2% (FT and centrifuge stability). Changes in the NaCl concentration also had minimal effects on the viscosity of the emulsions as shown in the plot.Figure 193 show photographs of silk & thickener formulations (0.8% xanthan gum - 5% oil - 0% mid mw silk (10% solution)). Separation noted in all conditions in the absence of soluble fibroin.Figure 194 shows photographs of silk & thickener formulations (0.8% xanthan gum - 5% oil - 0.2% solids mid mw silk). Results show better emulsion across all different oils with 0.2% of mid mw silk.Figure 195 shows photographs of silk & thickener formulations (silk / clay / thickener, 0.8% xanthan gum, 5% grapeseed oil, 0.1 % clay). Results show thicker and viscous emulsions with 1% solid mid mw silk.Figure 196 shows photographs of silk & thickener formulations ( 0.8% xanthan gum, 5% grapeseed oil, 0.1% clay). Results show emulsions are stable with 0.2 and 1% Mid MW Silk & Clay.Figure 197 is a photograph illustrating the viscosity of various emulsions with various soluble fibroin concentrations.Figure 198 shows optical photographs of the emulsions. Soluble fibroin results in reduced and more uniform droplet size.Figures 199 A- 199B are examples of the proteoglycan molecules. Fig. 199A is an illustration of the P1 and P2 molecules and how they become a hydrogel. Fig. 199B is an example of a proteoglycan molecule.Figure 200 is a photograph of an emulsified oil droplet.Figure 201 is a photograph of strand of hair before and after activated silk treatment.Figures 202A- 202B are graphs illustrating clinical efficiency of silk hydrogels on skin. Controlled release of hydration clinically supports improved 24hr skin hydration over baseline, similar to high molecular weight (Mw) HA. Hydrogel P2 significantly increased skin elasticity and firmness vs high Mw HA.Figure 203 shows photographs of clinical efficiency of silk hydrogels for hair. Silk hydrogels deliver the power of a keratin treatment without cross- linkers, heat or even the treatment itself and deliver keratin sealing and protection benefits in rinse- off products. Silk hydrogels show lasting hold results in non-toxic hair memory treatments, repairs split ends, and delivers all of the results associated with HA and hydrolyzed silk, but with enduring and lasting properties.Figure 203 shows photographs of Emulsified P 1 binding to skin keratin and hair keratin (green) in situ following multiple washes.Figure 204 shows photographs of P2 Hydrogel with emulsified oil (pink on right) penetrates epidermis following topical application. The effect results from the unique aliphatic biochemical composition of the Activated Silk™ peptide in P1 & P2 proteoglycans. Activated Silk™ peptide in P1 & P2 delivers maximum performance of delivered actives and effectively deliver previously unavailable actives, from those for anti-aging to wound healing and hydration.Figure 205 shows photographs of skin penetration of the Activated Silk™ peptide in P1 & P2.Figure 206 are graphs showing the water affinity of the P2 hydrogels and viscosity of P1 and P2 hydrogels.Figure 207 are graphs showing that P1 & P2 demonstrate superior spreadability as compared to HA’s viscous (sticky) feel without scarifice to initial hydrogel properties.Figure 208 is a photograph of P1 and P2 molecules before becoming a hydrogel.Figure 209 are graphs showing that silk hydrogels bind and deliver more oils and actives, naturally, with an expanded range of viscosity and haptics to choose from, all with excellent stability.Figure 210A- 210B illustrate the reconstitution of an emulsifier product described herein.Figure 211 is a graph showing viscosity of emulsions made with varying concentrations of 720X and 30% oil concentration.Figure 212 is a graph showing the shear thinning viscosity profile of emulsions made with 720X at varying oil concentrations.Figure 213 is a graph illustrating the thermal stability of emulsions made with 1% 720X after three weeks at 50 °C.Figures 214A- 214F illustrate Rheological properties of Activated Silk™ Hydrogel P1-alpha and P1-beta. (A): the viscosity of hydrogel P1-alpha and P1-beta emulsified product (20% oil) as a function of hydrogel concentration; (B): the viscosity of 1% hydrogel P1 -alpha and P1 -beta emulsified product at different oil content held by the hydrogel; (C): the response of the viscosity of P1-alpha and P1- beta at the concentrations of 0.25% and 1% to the shear rate and compared to that of Carbopol Fusion S20 and Sepimax Zen; (D): the response of modulus of P1 -alpha and P1 -beta at the concentrations of 0.25% and 1% to the oscillation stress and comparedto that of Carbopol Fusion S20 and Sepimax Zen; (E): the response of the viscosity of 1% P1-alpha emulsified formulations with various amount of oil to the shear rate; (F): viscosity of P1 -alpha emulsions in comparison to benchmark at 30% oil concentration.Figures 215A- 215D shows emulsification capability of hydrogel P1-alpha and P1-beta. (A): summary of the stability of emulsions made with 0.25% to 1.50% of P1-alpha and P1-beta and a oil content ranging from 1% to 80%; (B): the viscosity and texture of emulsions made with 1 % P 1 -alpha at 1 %, 20% and 50% oil levels; (C): the viscosity and texture of emulsions made with 1% P1-beta at 1%, 20% and 50% oil levels; (D): the viscosity and texture of emulsions made with 0.5% and 1.0% of P1 - alpha or P1 -beta with 50% of oil.Figures 216A-216B show Activated Silk™ hydrogel stability: the effect of (A) salt and (B) pH on the viscosity of emulsions made of 1% P1 -alpha and P1 -beta with 20% olive oil.Figure 217A is a table of emulsions made with Activated Silk™ hydrogels and compared to that of emulsions made with Olivem emulsifier at different concentrations. Figure 217B is a Cryo-SEM image of emulsion made with Activated Silk™ Hydrogel emulsifiers.Figures 218A- 218D illustrates Shear thinning viscosity test of hydrogel solutions (A), 0.5% concentration and B, 1% concentration) compared to HA solutions; Dehydration (C) and rehydration tests (D) of hydrogel compared to HA solution at 1% of concentration.]Figures 219A- 219B show how Activated Silk™ Hydrogels significantly improves hydration over 24 hours compared to t=0 baseline and because of their ability to bind to keratin and remain on skin. Activated Silk™ Hydrogels also improve skin firmness and elasticity.Figures 220A- 220B illustrate how confocal microscopy detected the binding of Activated silk hydrogel and different formulations with the hydrogel on hair. The silk was labeled as red and the hair was labeled as blue. Hair was incubated in the dark with hydrogels and formulations (from left to right: 1% Hydrogel P1 in H2O , 1% Hydrogel P2 in H2O, Hydrogel P1 shampoo, Hydrogel P2 shampoo, Hydrogel P1 conditioner, Hydrogel P2 conditioner, 1% Hydrogel P1 solution with 5% MCT oil, 1% Hydrogel P2 solution with 5% MCT oil) made with fluorescence labeled silk for ~1.5 h, followed by washing with water 6 times.Figure 221 illustrates the penetration of labeled silk in hair imaged by confocal microscopy. Images represent penetration of silk-594 (red) at ~25 μm depth into root, middle and tip of 50 μm hair strands (blue). Hair strands were treated with Hydrogel P1 / P2, Shampoo P1 / P2 or Emulsion P1 / P2. Positive control shows permanent red hair dye bound to hair strand and negative control shows no red intensity signal detected.Figures 222A- 222B illustrate split-end hair treated with Activated Silk Hydrogel (A, water was used as control); Split-end hair treated with Activated silk hydrogel followed by harsh conditions survival testing of high humidity (Bl, 40 °C, 90% RH), iron test (B2, 450 F for 10 seconds) and rinsing survival test (B3).Figure 223 illustrates curl retention over time (t = 0 hours and t = 20 hours) of bleached Caucasian straight hair treated with water, JMO spray fixant, and various concentrations of Activated silk hydrogels (0.25% and 1%).Figure 224 illustrates anti-frizz tests on Virgin dark brown curly hairs with different formulations (from left to right: untreated hair; SLES shampoo: 15% SLES; Hydrogel P2 shampoo: SLES shampoo with 1% of Activated silk hydrogel; Hydrogel P2 conditioner: 0.5% BTAC, 1% hydrogel, 5% MCT oil; Hydrogel P2 solution: 1% Activated silk hydrogel; Hydrogel P2 emulsion: 1% hydrogel, 5% MCT oil; Olaplex No.7 Bonding Oil).Figures 225A- 225B illustrate heat protection test on bleached Caucasian straight hair treated with hydrogel solution (A); Thermogravimetric analysis (TGA) test on the bleached Caucasian straight hair treated with hydrogel solution, with a commercial benchmark product as the control (B).Figure 226 shows moisture loss test on bleached Caucasian straight hair treated with hydrogel solutions, with water treated hair as a control.Figure 227 is an image of a tray-Frozen Activated Silk Hydrogel lyophilization using 10% feed solution.Figure 228 is an image of pellets from cryopelletization of Activated Silk Hydrogel Solutions.Figure 229 shows the results from Size Exclusion Chromatography (SEC) with a refractive index detector for (i) Fibroin Proteogly can-1, and the control of (ii) unreacted xanthan gum.Figure 230 presents the results of stability studies using centrifugation (5000 rpm, 20 min) for a 50% MCT oil mixture with 1% fibroin solution, 1% xanthan gum solution, and 1% Fibroin Proteoglycan- 1.Figure 231 illustrates that 1% of Fibroin Proteoglycan shows much higher storage modulus (G’) than the same concentration of fibroin solution and xanthan gum solution alone.DETAILED DESCRIPTIONIn some embodiments, compositions of the present disclosure include peptides compositions selected from compositions #1001 to #2450, having weight average molecular weights selected from about 1 kDa to about 145 kDa, and a poly dispersity selected from between 1 and about 5 (including, without limitation, a poly dispersity of 1), between 1 and about 1.5 (including, without limitation, a poly dispersity of 1), between about 1.5 and about 2, between about 1.5 and about 3, between about 2 and about 2.5, between about 2.5 and about 3, between about 3 and about 3.5. between about 3.5 and about 4, between about 4 and about 4.5. and between about 4.5 and about 5.Methods of making silk fibroin or silk fibroin protein fragments and their applications in various fields are known and are described for example in U.S. Patents Nos. 9,187.538, 9,511.012, 9,517,191. 9,522,107, 9.522,108, 9,545.369, and 10,166,177, 10,287,728 and 10,301,768, all of which are incorporated herein in their entireties. Raw silk from silkworm Bombyx mori is composed of two primary proteins: silk fibroin (approximately 75%) and sericin (approximately 25%). Silk fibroin is a fibrous protein with a semi-crystalline structure that provides stiffness and strength. As used herein, the term “silk fibroin” means the fibers of the cocoon of Bombyx mori having a weight average molecular weight of about 370,000 Da. The crude silkworm fiber consists of a double thread of fibroin. The adhesive substance holding these double fibers together is sericin. The silk fibroin is composed of a heavy chain having a weight average molecular weight of about 350,000 Da (H chain), and a light chain having a weight average molecular weight about 25,000 Da (L chain). Silk fibroin is an amphiphilic polymer with large hydrophobic domains occupying the major component of the polymer, which has a high molecular weight. The hydrophobic regions are interrupted by small hydrophilic spacers, and the N- and C- termini of the chains are also highly hydrophilic. The hydrophobic domains of the H-chain contain a repetitive hexapeptide sequence of Gly-Ala-Gly-Ala-Gly-Ser and repeats of Gly-Ala / Ser / Tyr dipeptides, which can form stable anti-parallel-sheet crystallites. The amino acid sequence of the L-chain is non-repetitive, so the L-chain is more hydrophilic and relatively elastic. The hydrophilic (Tyr, Ser) and hydrophobic (Gly, Ala) chain segments in silk fibroin molecules are arranged alternatively such that allows self-assembling of silk fibroin molecules.The disclosure provides peptide compositions, e.g., silk fibroin derived peptide compositions. International Publication No. WO 2024 / 1 19107 and International Publication No. WO 2024 / 059862 are incorporated by reference herein.As used herein, the term ‘‘fibroin” includes silk worm fibroin and insect or spider silk protein. In an embodiment, fibroin is obtained from Bombyx mori. Raw silk from Bombyx mori is composed of two primary proteins: silk fibroin (approximately 75%) and sericin (approximately 25%). As used herein, the term ’‘silk fibroin” means the fibers of the cocoon of Bombyx mori having a weight average molecular weight of about 370,000 Da. Methods of making silk fibroin protein fragments, and / or compositions thereof, are known and are described for example in U.S. Patents Nos. 9,187,538, 9,511,012, 9,517,191, 9,522,107, 9,522,108, 9,545,369, and 10,166,177.Recombinant silk described in patents and patent applications, incorporated by reference herein: US 2004590196. US 7,754,851, US 2007654470, US 7.951,908, US 2010785960, US 8,034,897, US 20090263430, US 2008226854, US 20090123967, US 2005712095, US 2007991037, US 20090162896, US 200885266, US 8,372,436, US 2007989907, US 2009267596, US 2010319542, US 2009265344, US 2012684607, US 2004583227. US 8,030,024, US 2006643569, US 7.868,146, US 2007991916, US 8,097,583, US 2006643200, US 8,729,238, US 8,877,903, US 20190062557, US 20160280960, US 20110201783, US 2008991916, US 2011986662, US 2012697729, US 20150328363, US 9,034,816, US 20130172478, US 9.217,017, US 20170202995, US 8,721,991. US 2008227498, US 9,233,067, US 8,288,512, US 2008161364, US 7.148,039, US 1999247806, US 2001861597, US 2004887100, US 9,481,719, US 8,765,688, US 200880705, US 2010809102, US 8,367,803, US 2010664902, US 7,569,660, US 1999138833, US 2000591632, US 20120065126, US 20100278882, US 2008161352, US 20100015070, US 2009513709, US 20090194317, US 2004559286, US 200589551, US 2008187824, US 20050266242, US 20050227322, and US 20044418.Recombinant silk is also described in other patents and patent applications, incorporated by reference herein: US 20190062557. US 20150284565, US 20130225476, US 20130172478, US 20130136779, US 20130109762, US 20120252294, US 20110230911, US 20110201783, US 20100298877, US 10,478,520, US 10,253,213, US 10,072,152, US 9,233,067, US 9,217,017, US 9,034,816, US 8,877,903, US 8,729,238, US 8,721,991, US 8,097,583, US 8,034,897, US 8.030,024, US 7.951,908, US 7,868.146, and US 7,754.851.Recombinant silk protein and / or methods described herein, may include one or more recombinant silk proteins described above or recited in U.S. Patent Nos. 8.173,772, 8,278,416, 8,618,255, 8,642,734, 8,691,581, 8.729,235, 9,115,204, 9,157,070, 9.309,299, 9,644.012, 9,708,376. 9,051,453, 9,617,315, 9.968,682, 9,689,089, 9,732,125, 9,856,308, 9,926,348, 10,065,997, 10,316,069, and 10,329,332; and U.S. Patent Publication Nos. 2009 / 0226969, 2011 / 0281273, 2012 / 0041177, 2013 / 0065278, 2013 / 0115698, 2013 / 0316376, 2014 / 0058066, 2014 / 0079674, 2014 / 0245923, 2015 / 0087046. 2015 / 0119554. 2015 / 0141618, 2015 / 0291673, 2015 / 0291674. 2015 / 0239587. 2015 / 0344542. 2015 / 0361144, 2015 / 0374833, 2015 / 0376247, 2016 / 0024464, 2017 / 0066804, 2017 / 0066805, 2015 / 0293076, 2016 / 0222174, 2017 / 0283474, 2017 / 0088675, 2019 / 0135880, 2015 / 0329587, 2019 / 0040109, 2019 / 0135881, 2019 / 0177363. 2019 / 0225646, 2019 / 0233481, 2019 / 0031842. 2018 / 0355120. 2019 / 0186050. 2019 / 0002644, 2020 / 0031887, 2018 / 0273590, 20191 / 094403, 2019 / 0031843, 2018 / 0251501, 2017 / 0066805, 2018 / 0127553, 2019 / 0329526, 2020 / 0031886, 2018 / 0080147, 2019 / 0352349, 2020 / 0043085, 2019 / 0144819, 2019 / 0228449. 2019 / 0340666, 2020 / 0000091, 2019 / 0194710, 2019 / 0151505. 2018 / 0265555. 2019 / 0352330, 2019 / 0248847, and 2019 / 0378191, the entirety of which are incorporated herein by reference.As used herein, the term “substantially free of inorganic residuals” means that the composition exhibits residuals of 0.1 % (w / w) or less. In an embodiment, substantially free of inorganic residuals refers to a composition that exhibits residuals of 0.05% (w / w) or less. In an embodiment, substantially free of inorganic residuals refers to a composition that exhibits residuals of 0.01 % (w / w) or less. In an embodiment, the amount of inorganic residuals is between 0 ppm (“non-detectable” or “ND”) and 1000 ppm. In an embodiment, the amount of inorganic residuals is ND to about 500 ppm. In an embodiment, the amount of inorganic residuals is ND to about 400 ppm. In an embodiment, the amount of inorganic residuals is ND to about 300ppm. In an embodiment, the amount of inorganic residuals is ND to about 200 ppm. In an embodiment, the amount of inorganic residuals is ND to about 100 ppm. In an embodiment, the amount of inorganic residuals is between 10 ppm and 1000 ppm.As used herein, the term “substantially free of organic residuals” means that the composition exhibits residuals of 0.1 % (w / w) or less, in an embodiment, substantially free of organic residuals refers to a composition that exhibits residuals of 0.05% (w / w) or less. In an embodiment, substantially free of organic residuals refers to a composition that exhibits residuals of 0.01 % (w / w) or less. In an embodiment, the amount of organic residuals is between 0 ppm (“non-detectable” or “ND”) and 1000 ppm. In an embodiment, the amount of organic residuals is ND to about 500 ppm. In an embodiment, the amount of organic residuals is ND to about 400 ppm. In an embodiment, the amount of organic residuals is ND to about 300 ppm. In an embodiment, the amount of organic residuals is ND to about 200 ppm. In an embodiment, the amount of organic residuals is ND to about 100 ppm. In an embodiment, the amount of organic residuals is between 10 ppm and 1000 ppm.Compositions of the present disclosure exhibit “biocompatibility” meaning that the compositions are compatible with living tissue or a living system by not being toxic, injurious, or physiologically reactive and not causing immunological rejection. Such biocompatibility can be evidenced by participants topically applying compositions of the present disclosure on their skin for an extended period of time. In an embodiment, the extended period of time is about 3 days. In an embodiment, the extended period of time is about 7 days, in an embodiment, the extended period of time is about 14 days, in an embodiment, the extended period of time is about 21 days. In an embodiment, the extended period of time is about 30 days. In an embodiment, the extended period of time is selected from the group consisting of about I month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, about 12 months, and indefinitely.Compositions of the present disclosure can be “hypoallergenic” meaning that they are relatively unlikely to cause an allergic reaction. Such hypoallergenicity can be evidenced by participants topically applying compositions of the present disclosure on their skin for an extended period of time. In an embodiment, the extended period of time is about 3 days. In an embodiment, the extended period of time is about 7 days. In an embodiment, the extended period of time is about 14 days. In anembodiment, the extended period of time is about 21 days. In an embodiment, the extended period of time is about 30 days. In an embodiment, the extended period of time is selected from the group consisting of about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6 months, about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, about 12 months, and indefinitely.In an embodiment, a peptide composition of the present disclosure has non- detectable levels of LiBr residuals. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is between 10 ppm and 1000 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is between 10 ppm and 300 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 25 ppm. In an embodiment, the amount of the Li Br residuals in a composition of the present disclosure is less than 50 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 75 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 100 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 200 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 300 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 400 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 500 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 600 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 700 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 800 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 900 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is less than 1000 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non- detectable to 500 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 450 ppm. In an embodiment, the amount of the LiBr residue in a composition of the present disclosure is non-detectable to 400 ppm. In an embodiment, the amount of the LiBrresiduals in a composition of the present disclosure is non-detectable to 350 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 300 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 250 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 200 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 150 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is non-detectable to 100 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is 100 ppm to 200 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is 200 ppm to 300 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is 300 ppm to 400 ppm. In an embodiment, the amount of the LiBr residuals in a composition of the present disclosure is 400 ppm to 500 ppm.In an embodiment, a peptide composition of the present disclosure has non- detectable levels of Na2CO3residuals. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 100 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 200 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 300 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 400 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 500 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 600 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 700 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 800 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 900 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is less than 1000 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is non-detectable to 500 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the presentdisclosure is non-detectable to 450 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is non-detectable to 400 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is non- detectable to 350 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is non-detectable to 300 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is non- detectable to 250 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is non-detectable to 200 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is non-detectable to 150 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is non-detectable to 100 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is 100 ppm to 200 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is 200 ppm to 300 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is 300 ppm to 400 ppm. In an embodiment, the amount of the Na2CO3residuals in a composition of the present disclosure is 400 ppm to 500 ppm.As used herein when referring to a number or a numerical range, the term “about"’ means that the stated number or numerical range is included together with numbers or numerical ranges within experimental variability, or within statistical experimental error from the stated number or numerical range, wherein the variation or error is from 0% to 15%, or from 0% to 10%, or from 0% to 5% of the stated number or numerical range.The silk proteins or fragments thereof, silk solutions or mixtures (e.g., SPF or SFS solutions or mixture), and the like, may be prepared according to the methods described in U.S. Patent Nos. 9,187,538, 9,522,107, 9,522,108, 9,511,012, 9,517,191, 9,545,369, and 10,166,177, and U.S. Patent Publication Nos. 2016 / 0222579 and 2016 / 0281294, and International Patent Publication Nos. WO 2016 / 090055 and WO 2017 / 011679, the entirety of which are incorporated herein by reference. Methods of using silk fibroin or silk fibroin fragments in coating applications are known and are described for example in U.S. Patents Nos. 10,287,728 and 10,301,768.Silk is a natural polymer produced by a variety of insects and spiders. Silk produced by Bombyx mori (silkworm) comprises a filament core protein, silk fibroin, and a glue-like coating consisting of a nonfilamentous protein, sericin. Silk fibroin isa FDA approved, edible, non-toxic, and relative inexpensive silkworm cocoon derived proteins. The structure and content of amino acids in the silk fibroin protein are very similar to the tissue of the human body.Methods of making silk fibroin protein fragments are known and are described for example in U.S. Patents Nos. 9,187,538, 9,511,012, 9,517,191, 9,522,107, 9,522,108, 9,545,369, and 10,166,177. Methods of using silk fibroin or silk fibroin fragments in coating applications, including coating applications of animal hair, are known and are described for example in U.S. Patent Application Publications Nos. 20160222579, and 20160281294. Compositions and methods of using silk fibroin or silk fibroin fragments in cosmetic applications are known and are described for example in U.S. Patent Application Publications Nos. 20180280274 and 20180008522, and International Patent Application Publication No. WO 2019005848. All of the publications cited herein are incorporated by reference herein in their entireties.The recent advancements in silk material technology include the emergence of the top-down and bottom-up engineering of silk cocoon, specifically regenerate cocoon into an aqueous silk solution and to use genetic engineering to produce recombinant silk with molecularly defined composition (Tran et al., A review of the emerging role of silk for the treatment of the eye, Pharm. Res., 2018, vol. 35, pp. 1- 16). In recent years, silk fibroin proteins are reported to have found applications in ocular tissue reconstruction, comeal tissue engineering and in ocular surface repair due to their biocompatibility, tunable properties, and transparency. Silk films have been found to support corneal cell growth and to develop stratified epithelial cell sheets equivalent to amniotic membrane substrates (Lawrence et al., Silk film biomaterials for comea tissue engineering. Biomaterials, 2009; 30(7): 1299-308; Harkin et al.. Silk fibroin in ocular tissue reconstruction, Biomaterials, 2011; Chirtla et al., Bombyx mori silk fibroin membranes as potential substrata for epithelial constructs used in the management of ocular surface disorders. Tissue Engineering Pan A, 2008; 14(7): 1203- 11.). Silk fibroin protein and hydrolyzed peptide fragments have been shown to inhibit transcription and upstream activation of NF-KB protein subunits and proinflammatory molecules that are classically under the control ofNF- KB (Hayden et al., Cell Research, 2011. 21(2): 223-244; Chon et al., International Journal of Molecular Medicine, 2012. 30(5): 1203-1210; Kim et al., J. Neurosurg., 2011. 1 14(2): 485-90; J. Microbiol. BiotechnoL 2012. 22(4): 494-500).DefinitionsAs used in the preceding sections and throughout the rest of this specification, unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one skilled in the art to which this disclosure belongs. All patents and publications referred to herein are incorporated by reference in their entireties.All percentages, parts and ratios are based upon the total weight of the eye care compositions of the present disclosure, unless otherwise specified. All such weights as they pertain to listed ingredients are based on the active level and, therefore, do not include solvents or by-products that may be included in commercially available materials, unless otherwise specified. The term “weight percent” may be denoted as “wt. %” or % w / w herein.As used herein, the term “a”, “an”, or “the” generally is construed to cover both the singular and the plural forms.The term “about” as used herein, generally refers to a particular numeric value that is within an acceptable error range as determined by one of ordinary skill in the art, which will depend in part on how the numeric value is measured or determined, i.e., the limitations of the measurement system. For example, “about” can mean zero variation, and a range of ±20%, ±10%, or ±5% of a given numeric value.As used herein, the term “dermatologically acceptable carrier” means a carrier suitable for use in contact with mammalian keratinous tissue without causing any adverse effects such as undue toxicity, incompatibility, instability, allergic response, for example. A dermatologically acceptable carrier may include, without limitations, water, liquid or solid emollients, humectants, solvents, and the like.As used herein, the term “hydrophilic-lipophilic balance” (HLB) of a surfactant and / or emulsifier is a measure of the degree to which it is hydrophilic or hydrophobic, as determined by calculating values for the different regions of the molecule, as described by Griffin’s method HLB = 20 * Mh / M, where Mhis the molecular mass of the hydrophilic portion of the surfactant and / or emulsifier, and M is the molecular mass of the entire surfactant and / or emulsifier molecule, giving a result on a scale of 0 to 20. A HLB value of 0 corresponds to a completely lipophilic molecule, and a value of 20 corresponds to a completely hydrophilic molecule. The HLB value can be used to predict the surfactant and / or emulsifier properties of amolecule: HLB < 10: Lipid-soluble (water-insoluble), HLB >10: Water-soluble (lipid- insoluble), HLB = 1-3: anti-foaming agent, 3-6: W / O (water-in-oil) emulsifier, 7-9: wetting and spreading agent, 8-16: O / W (oil-in-water) emulsifier, 13-16: detergent, 16-18: solubilizer or hydrotrope.As used herein, “average weight average molecular weight” refers to an average of two or more values of weight average molecular weight of silk fibroin or fragments thereof of the same compositions, the two or more values determined by two or more separate experimental readings.As used herein, the term polymer "polydispersity (PD)” is generally used as a measure of the broadness of a molecular weight distribution of a polymer, and is defined bv the formula polvdispersitv PD = — .As used herein, the term “substantially homogeneous” may refer to silk fibroin- based protein fragments that are distributed in a normal distribution about an identified molecular weight. As used herein, the term “substantially homogeneous” may refer to an even distribution of a component or an additive, for example, silk fibroin fragments, dermatologically acceptable carrier, etc., throughout a composition of the present disclosure.As used herein, the terms “silk fibroin peptide,” “silk fibroin protein fragment,” and “silk fibroin fragment” are used interchangeably. Molecular weight or number of amino acids units are defined when molecular size becomes an important parameter.As used herein, the term “fast-dissolving solid forms” refers to fast-dissolving solid forms including freeze dried forms (cakes, wafers, thin films), and compressed tablets.As used herein, the terms “peptide” or “protein” refers to a chain of amino acids that are held together by peptide bonds (also called amide bonds). The basic distinguishing factors for proteins and peptides are size and structure. Peptides are smaller than proteins. Traditionally, peptides are defined as molecules that consist of between 2 and 50 amino acids, whereas proteins are made up of 50 or more amino acids. In addition, peptides tend to be less well defined in structure than proteins, which can adopt complex conformations known as secondary, tertiary, and quaternary structures.As used herein, the term ‘‘fibroin” or “silk protein” is a type of structural protein produced by certain spider and insect species that produce silk (See definition provided in WIPO Pearl-WIPO’s Multilingual Terminology Portal database, https: / / wipopearl.wipo.int / en / linguistic). Fibroin may include silkworm fibroin, insect or spider silk protein (e.g., spidroin), recombinant spider protein, silk proteins present in other spider silk types, e.g., tubuliform silk protein (TuSP). flagelliform silk protein, minor ampullate silk proteins, aciniform silk protein, pyriform silk protein, aggregate silk glue), silkworm fibroin produced by genetically modified silkworm, or recombinant silkworm fibroin.As used herein, the term “silk fibroin” refers to silkworm fibroin, silk fibroin produced by genetically modified silkworm, or recombinant silkworm fibroin (See (1) Narayan Ed., Encyclopedia of Biomedical Engineering, Vol. 2, Elsevier, 2019; (2) Kobayashi et al. Eds, Encyclopedia of Polymeric Nanomaterials, Springer, 2014, https: / / link.springer.com / referenceworkentiy710.1007%2F978-3-642-36199-9_323-l). In an embodiment, silk fibroin is obtained from Bombyx mori.The term “solid solution” as used herein, refers to the active agent molecularly dissolved in the solid excipient matrix such as hydrophobic polymers, wherein the active agent is miscible with the polymer matrix excipient.The term “solid dispersion” as used herein, refers to the active agent dispersed as crystalline or amorphous particles, wherein the active agent is dispersed in an amorphous polymer and is distributed at random between the polymer matrix excipient.As used herein, the term “substantially homogeneous” may refer to silk fibroin-based protein fragments that are distributed in a normal distribution about an identified molecular weight. As used herein, the term “substantially homogeneous” may also refer to an even distribution of a component or an additive, for example, silk fibroin-based protein fragments, dermatologically acceptable carrier, etc., throughout the silk eye care composition.As used herein, the term “surface tension” refers to the tendency of fluid surfaces to shrink into the minimum surface area possible. At liquid-air interfaces, surface tension results from the greater attraction of liquid molecules to each other (due to cohesion) than to the molecules in the air (due to adhesion). The net effect is an inward force at its surface that causes the liquid to behave as if its surface were covered with a stretched elastic membrane. Because of the relatively high attraction ofwater molecules to each other through a web of hydrogen bonds, water has a higher surface tension (72.8 mN / m at 20 °C) than most other liquids.SPF Definitions and PropertiesAs used herein, “silk protein fragments” (SPF) include, without limitation, one or more of: “silk fibroin fragments” as defined herein; “recombinant silk fragments” as defined herein; “spider silk fragments” as defined herein; “silk fibroin-like protein fragments” as defined herein; “chemically modified silk fragments” as defined herein; “sericin or sericin fragments” as defined herein; and / or modified silk fragments disclosed herein, e.g., and without limitation, fibroin peptides and / or protein fragments wherein at least one of the amino acids is modified, substituted, or replaced as disclosed herein. SPF may have any molecular weight values or ranges described herein, and any poly dispersity values or ranges described herein.SPF Molecular Weight and Poly dispersityIn an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 1 to about 5 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 5 to about 10 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 10 to about 15 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 1 to about 20 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 14 to about 30 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 20 to about 25 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 25 to about 30 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 30 to about 35 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 35 to about 40 kDa. Inan embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 39 to about 54 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 40 to about 45 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 45 to about 50 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 50 to about 55 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 55 to about 60 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 60 to about 65 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 65 to about 70 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 70 to about 75 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 75 to about 80 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 80 to about 85 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 85 to about 90 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 90 to about 95 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 95 to about 100 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 100 to about 105 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 105 to about 110 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 110 to about 115 kDa. In an embodiment, a composition of thepresent disclosure includes SPF having an average weight average molecular weight selected from between about 115 to about 120 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 120 to about 125 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 125 to about 130 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 130 to about 135 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 135 to about 140 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 140 to about 145 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 145 to about 150 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 150 to about 155 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 155 to about 160 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 160 to about 165 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 165 to about 170 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 170 to about 175 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 175 to about 180 kDa.In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 180 to about 185 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 185 to about 190 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 190 to about 195 kDa. In an embodiment, a composition of the presentdisclosure includes SPF having an average weight average molecular weight selected from between about 195 to about 200 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 200 to about 205 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 205 to about 210 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 210 to about 215 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 215 to about 220 kDa.In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 220 to about 225 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 225 to about 230 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 230 to about 235 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 235 to about 240 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 240 to about 245 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 245 to about 250 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 250 to about 255 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 255 to about 260 kDa.In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 260 to about 265 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 265 to about 270 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 270 to about 275 kDa. In an embodiment, a composition of the presentdisclosure includes SPF having an average weight average molecular weight selected from between about 275 to about 280 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 280 to about 285 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 285 to about 290 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 290 to about 295 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 295 to about 300 kDa.In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 300 to about 305 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 305 to about 310 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 310 to about 315 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 315 to about 320 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 320 to about 325 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 325 to about 330 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 330 to about 335 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 335 to about 340 kDa.In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 340 to about 345 kDa. In an embodiment, a composition of the present disclosure includes SPF having an average weight average molecular weight selected from between about 345 to about 350 kDa.In some embodiments, compositions of the present disclosure include SPF compositions selected from compositions #1001 to #2450, having weight averagemolecular weights selected from about 1 kDa to about 145 kDa. and a poly dispersity selected from between 1 and about 5 (including, without limitation, a poly dispersity of 1), between 1 and about 1.5 (including, without limitation, a polydispersity of 1), between about 1.5 and about 2, between about 1.5 and about 3, between about 2 and about 2.5, between about 2.5 and about 3, between about 3 and about 3.5, between about 3.5 and about 4, between about 4 and about 4.5. and between about 4.5 and about 5:In an embodiment, SPF in a composition of the present disclosure have a polydispersity selected from between 1 to about 5.0, including, without limitation, a poly dispersity of 1. In an embodiment, SPF in a composition of the present disclosure have a polydispersity selected from between about 1.5 to about 3.0. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity selected from between 1 to about 1.5, including, without limitation, a polydispersity of 1. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity selected from between about 1.5 to about 2.0. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity selected from between about 2.0 to about 2.5. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity selected from between about 2.5 to about 3.0.In an embodiment, SPF in a composition of the present disclosure have a poly dispersity selected from between about 3.0 to about 3.5. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity selected from between about 3.5 to about 4.0. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity selected from between about 4.0 to about 4.5.In an embodiment, SPF in a composition of the present disclosure have a poly dispersity selected from between about 4.5 to about 5.0.In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of 1. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 1.1. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 1.2. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 1.3. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 1.4. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 1.5. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 1.6. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 1.7. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 1.8. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 1.9. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 2.0. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 2.1. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 2.2. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 2.3. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 2.4. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 2.5. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 2.6. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 2.7. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 2.8. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 2.9. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 3.0. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 3.1. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 3.2. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 3.3. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 3.4. In an embodiment, SPF in a composition of the present disclosure have apoly dispersity of about 3.5. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 3.6. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 3.7. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 3.8. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 3.9. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 4.0. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 4.1. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 4.2. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 4.3. In an embodiment, SPF in a composition of the present disclosure have a polydispersity of about 4.4. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 4.5. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 4.6. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 4.7. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 4.8. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 4.9. In an embodiment, SPF in a composition of the present disclosure have a poly dispersity of about 5.0.Silk Fibroin FragmentsMethods of making silk fibroin or silk fibroin protein fragments and their applications in various fields are known and are described for example in U.S. Patents Nos. 9,187.538, 9,511,012, 9,517,191, 9,522,107, 9.522,108, 9,545.369, and 10.166.177, 10,287,728 and 10,301.768. all of which are incorporated herein in their entireties. Raw silk from silkw orm Bombyx mori is composed of two primary proteins: silk fibroin (approximately 75%) and sericin (approximately 25%). Silk fibroin is a fibrous protein with a semi-crystalline structure that provides stiffness and strength. As used herein, the term "‘silk fibroin" means the fibers of the cocoon of Bombyx mori having a weight average molecular weight of about 370,000 Da. The crude silkw orm fiber consists of a double thread of fibroin. The adhesive substance holding these double fibers together is sericin. The silk fibroin is composed of a heavy chain having a weight average molecular weight of about 350,000 Da (H chain), and alight chain having a weight average molecular weight about 25,000 Da (L chain). Silk fibroin is an amphiphilic polymer with large hydrophobic domains occupying the major component of the polymer, which has a high molecular weight. The hydrophobic regions are interrupted by small hydrophilic spacers, and the N- and C- termini of the chains are also highly hydrophilic. The hydrophobic domains of the Id- chain contain a repetitive hexapeptide sequence of Gly-Ala-Gly-Ala-Gly-Ser and repeats of Gly-Ala / Ser / Tyr dipeptides, which can form stable anti-parallel-sheet crystallites. The amino acid sequence of the L-chain is non-repetitive, so the L-chain is more hydrophilic and relatively elastic. The hydrophilic (Tyr, Ser) and hydrophobic (Gly, Ala) chain segments in silk fibroin molecules are arranged alternatively such that allows self-assembling of silk fibroin molecules.Provided herein are methods for producing pure and highly scalable silk fibroin-protein fragment mixture solutions that may be used across multiple industries for a variety of applications. Without wishing to be bound by any particular theory, it is believed that these methods are equally applicable to fragmentation of any SPF described herein, including without limitation recombinant silk proteins, and fragmentation of silk-like or fibroin-like proteins.As used herein, the term “fibroin” includes silk worm fibroin and insect or spider silk protein. In an embodiment, fibroin is obtained from Bombyx mori. Raw silk from Bombyx mori is composed of two primary proteins: silk fibroin (approximately 75%) and sericin (approximately 25%). Silk fibroin is a fibrous protein with a semi-crystalline structure that provides stiffness and strength. As used herein, the term “silk fibroin” means the fibers of the cocoon of Bombyx mori having a weight average molecular weight of about 370,000 Da. Conversion of these insoluble silk fibroin fibrils into water-soluble silk fibroin protein fragments requires the addition of a concentrated neutral salt (e.g., 8-10 M lithium bromide), which interferes with inter- and intramolecular ionic and hydrogen bonding that would otherwise render the fibroin protein insoluble in water. Methods of making silk fibroin protein fragments, and / or compositions thereof, are known and are described for example in U.S. Patents Nos. 9,187,538, 9,511,012, 9,517,191, 9,522,107, 9,522,108, 9,545,369, and 10,166,177.FIG. 73 is a flow chart showing generic embodiments for producing some embodiments of the present disclosure. It should be understood that not all of the steps illustrated are necessarily required to fabricate all silk solutions of the presentdisclosure. As illustrated in FIG. 74, step A, cocoons (heat-treated or non-heat- treated), silk fibers, silk powder, spider silk or recombinant spider silk can be used as the silk source.FIG. 74 is a flow chart showing generic embodiments for various parameters that can be modified during the process of producing a silk protein fragment solution of the present disclosure during the extraction and the dissolution steps.In some embodiments, specifically disclosed herein, molecular weight of the silk fibroin protein fragment composition was determined using High Pressure Liquid Chromatography (HPLC) with a Refractive Index Detector (RID), and polydispersity was calculated using Cirrus GPC Online GPC / SEC Software Version 3.3 (Agilent). In some embodiments specifically disclosed herein, molecular weight of the silk fibroin protein fragment composition was determined using SEC-MALS methods.Data analysis and calculations - Calculation of Average Molecular Weight using Cirrus SoftwareUpload the chromatography data files of the standards and the analytical samples into Cirrus SEC data collection and molecular weight analysis software. Calculate the weight average molecular weight (Mw), number average molecular weight (Mn), peak average molecular weight (Mp), and polydispersity for each injection of the sample. Spider Silk FragmentsSpider silks are natural polymers that consist of three domains: a repetitive middle core domain that dominates the protein chain, and non-repetitive N-terminal and C-terminal domains. The large core domain is organized in a block copolymer- like arrangement, in which two basic sequences, crystalline [poly(A) or poly(GA)] and less crystalline (GGX or GPGXX) polypeptides alternate. Dragline silk is the protein complex composed of major ampullate dragline silk protein 1 (MaSpl) and major ampullate dragline silk protein 2 (MaSp2). Both silks are approximately 3500 amino acid long. MaSp1 can be found in the fiber core and the periphery, whereas MaSp2 forms clusters in certain core areas. The large central domains of MaSp1 and MaSp2 are organized in block copolymer-like arrangements, in which two basic sequences, crystalline [poly(A) or poly(GA)] and less crystalline (GGX or GPGXX) polypeptides alternate in core domain. Specific secondary structures have been assigned to poly(A) / (GA), GGX and GPGXX motifs including β-sheet, a-helix and β-spiral respectively. The primary sequence, composition and secondary structuralelements of the repetitive core domain are responsible for mechanical properties of spider silks; whereas, non-repetitive N- and C-terminal domains are essential for the storage of liquid silk dope in a lumen and fiber formation in a spinning duct.The main difference between MaSp1 and MaSp2 is the presence of proline (P) residues accounting for 15% of the total amino acid content in MaSp2, whereas MaSp1 is proline-free. By calculating the number of proline residues in N. clavipes dragline silk, it is possible to estimate the presence of the two proteins in fibers; 81% MaSp1 and 19% MaSp2. Different spiders have different ratios of MaSp1 and MaSp2. For example, a dragline silk fiber from the orb weaver Argiope aurantia contains 41% MaSp1 and 59% MaSp2. Such changes in the ratios of major ampullate silks can dictate the performance of the silk fiber.At least seven different types of silk proteins are known for one orb-weaver species of spider. Silks differ in primary sequence, physical properties and functions. For example, dragline silks used to build frames, radii and lifelines are known for outstanding mechanical properties including strength, toughness and elasticity. On an equal weight basis, spider silk has a higher toughness than steel and Kevlar.Flageliform silk found in capture spirals has extensibility of up to 500%. Minor ampullate silk, w hich is found in auxiliary spirals of the orb-web and in prey wrapping, possesses high toughness and strength almost similar to major ampullate silks, but does not supercontract in water.Spider silks are known for their high tensile strength and toughness. The recombinant silk proteins also confer advantageous properties to cosmetic or dermatological compositions, in particular to be able to improve the hydrating or softening action, good film forming properly and low surface density. Diverse and unique biomechanical properties together with biocompatibility and a slow rate of degradation make spider silks excellent candidates as biomaterials for tissue engineering, guided tissue repair and drug delivery, for cosmetic products (e.g. nail and hair strengthened skin care products), and industrial materials (e.g. nanowires, nanofibers, surface coatings).In an embodiment, a silk protein may include a polypeptide derived from natural spider silk proteins. The polypeptide is not limited particularly as long as it is derived from natural spider silk proteins, and examples of the polypeptide include natural spider silk proteins and recombinant spider silk proteins such as variants, analogs, derivatives or the like of the natural spider silk proteins. In terms of excellenttenacity, the polypeptide may be derived from major dragline silk proteins produced in major ampullate glands of spiders. Examples of the major dragline silk proteins include major ampullate spidroin MaSpl and MaSp2 from Nephila clavipes, and ADF3 and ADF4 fromAreneus diadematus, etc. Examples of the polypeptide derived from major dragline silk proteins include variants, analogs, derivatives or the like of the major dragline silk proteins. Further, the polypeptide may be derived from flagelliform silk proteins produced in flagelliform glands of spiders. Examples of the flagelliform silk proteins include flagelliform silk proteins derived from Nephila clavipes. etc.Examples of the polypeptide derived from major dragline silk proteins include a polypeptide containing two or more units of an amino acid sequence represented by the formula 1: REP1-REP2 (1), preferably a polypeptide containing five or more units thereof, and more preferably a polypeptide containing ten or more units thereof. Alternatively, the polypeptide derived from major dragline silk proteins may be a polypeptide that contains units of the amino acid sequence represented by the formula 1 : REP1-REP2 (1) and that has, at a C-terminal. an ammo acid sequence represented by any of SEQ ID NOS: 1 to 3 of U.S. Patent No. 9,051,453, which is incorporated by reference herein in its entirety, or an amino acid sequence having a homology of 90% or more with the amino acid sequence represented by any of SEQ ID NOS: 1 to 3 of U.S. Patent No. 9,051,453. which is incorporated by reference herein in its entirety. In the polypeptide derived from major dragline silk proteins, units of the amino acid sequence represented by the formula 1: REP1-REP2 (1) may be the same or may be different from each other. In the case of producing a recombinant protein using a microbe such as Escherichia coli as a host, the molecular weight of the polypeptide derived from major dragline silk proteins is 500 kDa or less, or 300 kDa or less, or 200 kDa or less, in terms of productivity .In the formula (1), the REP1 indicates poly alanine. In the REP1, the number of alanine residues arranged in succession is preferably 2 or more, more preferably 3 or more, further preferably 4 or more, and particularly preferably 5 or more. Further, in the REP1, the number of alanine residues arranged in succession is preferably 20 or less, more preferably 16 or less, further preferably 12 or less, and particularly preferably 10 or less. In the formula (1), the REP2 is an amino acid sequence composed of 10 to 200 amino acid residues. The total number of glycine, serine, glutamine and alanine residues contained in the amino acid sequence is 40% or more,preferably 60% or more, and more preferably 70% or more with respect to the total number of amino acid residues contained therein.In the major dragline silk, the REP1 corresponds to a crystal region in a fiber where a crystal β sheet is formed, and the REP2 corresponds to an amorphous region in a fiber where most of the parts lack regular configurations and that has more flexibility. Further, the [REP1-REP2] corresponds to a repetitious region (repetitive sequence) composed of the crystal region and the amorphous region, which is a characteristic sequence of dragline silk proteins.Recombinant Silk FragmentsIn some embodiments, the recombinant silk protein refers to recombinant spider silk polypeptides, recombinant insect silk polypeptides, or recombinant mussel silk polypeptides. In some embodiments, the recombinant silk protein fragment disclosed herein include recombinant spider silk polypeptides of Araneidae or Araneoids. or recombinant insect silk polypeptides of Bombyx mori. In some embodiments, the recombinant silk protein fragment disclosed herein include recombinant spider silk polypeptides of Araneidae or Araneoids. In some embodiments, the recombinant silk protein fragment disclosed herein include block copolymer having repetitive units derived from natural spider silk polypeptides of Araneidae or Araneoids. In some embodiments, the recombinant silk protein fragment disclosed herein include block copolymer having synthetic repetitive units derived from spider silk polypeptides of Araneidae ox Araneoids and non-repetitive units derived from natural repetitive units of spider silk polypeptides of Araneidae or Araneoids.Recent advances in genetic engineering have provided a route to produce various types of recombinant silk proteins. Recombinant DNA technology has been used to provide a more practical source of silk proteins. As used herein “recombinant silk protein” refers to synthetic proteins produced heterologously in prokaryotic or eukaryotic expression systems using genetic engineering methods.Various methods for synthesizing recombinant silk peptides are known and have been described by Ausubel et al., Current Protocols in Molecular Biology § 8 (John Wiley & Sons 1987, (1990)), incorporated herein by reference. A gram - negative, rod-shaped bacterium E. coli is a well-established host for industrial scale production of proteins. Therefore, the majority of recombinant silks have beenproduced in E. coli. E. coli which is easy to manipulate, has a short generation time, is relatively low cost and can be scaled up for larger amounts protein production.The recombinant silk proteins can be produced by transformed prokaryotic or eukaryotic systems containing the cDNA coding for a silk protein, for a fragment of this protein or for an analog of such a protein. The recombinant DNA approach enables the production of recombinant silks with programmed sequences, secondary structures, architectures and precise molecular weight. There are four main steps in the process: (i) design and assembly of synthetic silk-like genes into genetic ‘cassettes’, (ii) insertion of this segment into a DNA recombinant vector, (iii) transformation of this recombinant DNA molecule into a host cell and (iv) expression and purification of the selected clones.The term '‘recombinant vectors”, as used herein, includes any vectors known to the skilled person including plasmid vectors, cosmid vectors, phage vectors such as lambda phage, viral vectors such as adenoviral or baculoviral vectors, or artificial chromosome vectors such as bacterial artificial chromosomes (BAC). yeast artificial chromosomes (YAC), or P1 artificial chromosomes (PAC). Said vectors include expression as well as cloning vectors. Expression vectors comprise plasmids as well as viral vectors and generally contain a desired coding sequence and appropriate DNA sequences necessary for the expression of the operably linked coding sequence in a particular host organism (e.g., bacteria, yeast, or plant) or in in vitro expression systems. Cloning vectors are generally used to engineer and amplify a certain desired DNA fragment and may lack functional sequences needed for expression of the desired DNA fragments.The prokaryotic systems include Gram-negative bacteria or Gram-positive bacteria. The prokaryotic expression vectors can include an origin of replication which can be recognized by the host organism, a homologous or heterologous promoter which is functional in the said host, the DNA sequence coding for the spider silk protein, for a fragment of this protein or for an analogous protein. Nonlimiting examples of prokaryotic expression organisms are Escherichia coli. Bacillus subtilis. Bacillus megaterium, Corynebacterium glutamicum, Anabaena, Caulobacter, Gluconobacter, Rhodobacter, Pseudomonas, Para coccus. Bacillus (e.g. Bacillus subtilis) Brevibacterium, Corynebacterium, Rhizobium (Sinorhizobium), Flavobacterium, Klebsiella, Enterobacter, Lactobacillus, Lactococcus, Methylobacterium. Propionibacterium, Staphylococcus or Streptomyces cells.The eukaryotic systems include yeasts and insect, mammalian or plant cells. In this case, the expression vectors can include a yeast plasmid origin of replication or an autonomous replication sequence, a promoter, a DNA sequence coding for a spider silk protein, for a fragment or for an analogous protein, a polyadenylation sequence, a transcription termination site and, lastly, a selection gene. Nonlimiting examples of eukaryotic expression organisms include yeasts, such as Saccharomyces cerevisiae, Pichia pastoris, basidiosporogenous, ascosporogenous . filamentous fungi, such as Aspergillus niger, Aspergillus oryzae, Aspergillus nidulans, Trichoderma reesei, Acremonium chrysogenum, Candida, Hansenula, Kluyveromyces , Saccharomyces (e.g. Saccharomyces cerevisiae), Schizosaccharomyces, Pichia (e.g. Pichia pastoris) or Yarrowia cells etc., mammalian cells, such as HeLa cells, COS cells, CHO cells etc., insect cells, such as Sf9 cells, MEL cells, etc., ‘'insect host cells” such as Spodoptera frugiperda or Trichoplusia ni cells. SF9 cells, SF-21 cells or High-Five cells, wherein SF-9 and SF-21 are ovarian cells from Spodoptera frugiperda, and High-Five cells are egg cells from Trichoplusia ni., “plant host cells”, such as tobacco, potato or pea cells.A variety of heterologous host systems have been explored to produce different types of recombinant silks. Recombinant partial spidroins as well as engineered silks have been cloned and expressed in bacteria (Escherichia coli), yeast (Pichia pastoris , insects (silkworm larvae), plants (tobacco, soybean, potato. Arabidopsis), mammalian cell lines (BHT / hamster) and transgenic animals (mice, goats). Most of the silk proteins are produced with an N- or C-terminal His-tags to make purification simple and produce enough amounts of the protein.In some embodiments, the host suitable for expressing the recombinant spider silk protein using heterogeneous system may include transgenic animals and plants. In some embodiments, the host suitable for expressing the recombinant spider silk protein using heterogeneous system comprises bacteria, yeasts, mammalian cell lines. In some embodiments, the host suitable for expressing the recombinant spider silk protein using heterogeneous system comprises E. coli. In some embodiments, the host suitable for expressing the recombinant spider silk protein using heterogeneous system comprises transgenic B. mori silkworm generated using genome editing technologies (e.g., CRISPR).The recombinant silk protein in this disclosure comprises synthetic proteins which are based on repeat units of natural silk proteins. Besides the syntheticrepetitive silk protein sequences, these can additionally comprise one or more natural nonrepetitive silk protein sequences.In some embodiments, '‘recombinant silk protein” refers to recombinant silkworm silk protein or fragments thereof. The recombinant production of silk fibroin and silk sericin has been reported. A variety of hosts are used for the production including E. coli, Sacchromyces cerevisiae, Pseudomonas sp. , Rhodopseudomonas sp. , Bacillus sp.. and Strepomyces. See EP 0230702, which is incorporate by reference herein by its entirety.Provided herein also include design and biological-synthesis of silk fibroin protein-like multiblock polymer comprising GAGAGX hexapeptide (X is A, Y, V or S) derived from the repetitive domain of B. mori silk heavy chain (H chain) In some embodiments, this disclosure provides silk protein-like multiblock polymers derived from the repetitive domain of B. mori silk heavy chain (H chain) comprising the GAGAGS hexapeptide repeating units. The GAGAGS hexapeptide is the core unit of H-chain and plays an important role in the formation of crystalline domains. The silk protein-like multiblock polymers containing the GAGAGS hexapeptide repeating units spontaneously aggregate into β-sheet structures, similar to natural silk fibroin protein, where in the silk protein-like multiblock polymers having any weight average molecular weight described herein.In some embodiments, this disclosure provides silk-peptide like multiblock copolymers composed of the GAGAGS hexapeptide repetitive fragment derived from H chain of B. mori silk heavy chain and mammalian elastin VPGVG motif produced by E. coli. In some embodiments, this disclosure provides fusion silk fibroin proteins composed of the GAGAGS hexapeptide repetitive fragment derived from H chain of B. mori silk heavy chain and GVGVP produced by E. coli, where in the silk protein- like multiblock polymers having any weight average molecular weight described herein.In some embodiments, this disclosure provides B. mori silkworm recombinant proteins composed of the (GAGAGS)i6 repetitive fragment. In some embodiments, this disclosure provides recombinant proteins composed of the (GAGAGS)16repetitive fragment and the non-repetitive (GAGAGS)16-F-COOH, (GAGAGS)16-F- F-COOH, (GAGAGS)16-F-F-F-COOH, (GAGAGS)16-F-F-F-F-COOH, (GAGAGS)16-F-F-F-F-F-F-F-F-COOH, (GAGAGS)16-F-F-F-F-F-F-F-F-F-F-F-F- COOH produced by E. coli, where F has the following amino acid sequenceSGFGPVANGGSGEASSESDFGSSGFGPVANASSGEASSESDFAG, and where in the silk protein-like multiblock polymers having any weight average molecular weight described herein.In some embodiments, “recombinant silk protein” refers to recombinant spider silk protein or fragments thereof. The productions of recombinant spider silk proteins based on a partial cDNA clone have been reported. The recombinant spider silk proteins produced as such comprise a portion of the repetitive sequence derived from a dragline spider silk protein, Spidroin 7, from the spider Nephila clavipes. see Xu et al. (Proc. Natl. Acad. Sci. U.S.A., 87:7120-7124 (1990). cDNA clone encoding a portion of the repeating sequence of a second fibroin protein, Spidroin 2. from dragline silk of Nephila clavipes and the recombinant synthesis thereof is described in J. Biol. Chem.. 1992, volume 267, pp. 19320-19324. The recombinant synthesis of spider silk proteins including protein fragments and variants of Nephila clavipes from transformed E. coli is described in U.S. Pat. Nos. 5,728,810 and 5,989,894. cDNA clones encoding minor ampullate spider silk proteins and the expression thereof is described in U.S. Pat. Nos. 5,733,771 and 5.756,677. cDNA clone encoding the flagelliform silk protein from an orb-web spinning spider is described in U.S. Pat. No. 5,994,099. U.S. Pat. No. 6,268,169 describes the recombinant synthesis of spider silk like proteins derived from the repeating peptide sequence found in the natural spider dragline of Nephila clavipes by E. coli, Bacillus subtilis, andPichia pastoris recombinant expression systems. WO 03 / 020916 describes the cDNA clone encoding and recombinant production of spider spider silk proteins having repeative sequences derived from the major ampullate glands of Nephila madagascariensis, Nephila senegalensis, Tetragnatha kauaiensis, Tetragnatha versicolor, Argiope aurantia, Argiope trifasciata, Gasteracantha mammosa, and Latrodectus geometricus. the flagelliform glands of Argiope trifasciata, the ampullate glands of Dolomedes ienebrosus. two sets of silk glands from Plectreurys tristis, and the silk glands of the mygalomorph Euagrus chisoseus. Each of the above reference is incorporated herein by reference in its entirety.In some embodiments, the recombinant spider silk protein is a hybrid protein of a spider silk protein and an insect silk protein, a spider silk protein and collagen, a spider silk protein and resilin, or a spider silk protein and keratin. The spider silk repetitive unit comprises or consists of an amino acid sequence of a region that comprises or consists of at least one peptide motif that repetitively occurs within anaturally occurring major ampullate gland polypeptide, such as a dragline spider silk polypeptide, a minor ampullate gland polypeptide, a flagelliform polypeptide, an aggregate spider silk polypeptide, an aciniform spider silk polypeptide or a pyriform spider silk polypeptide.In some embodiments, the recombinant spider silk protein in this disclosure comprises synthetic spider silk proteins derived from repetitive units of natural spider silk proteins, consensus sequence, and optionally one or more natural non-repetitive spider silk protein sequences. The repeated units of natural spider silk polypeptide may include dragline spider silk polypeptides or flagelliform spider silk polypeptides of Araneidae or Araneoids .As used herein, the spider silk "‘repetitive unit” comprises or consists of at least one peptide motif that repetitively occurs within a naturally occurring major ampullate gland polypeptide, such as a dragline spider silk polypeptide, a minor ampullate gland polypeptide, a flagelliform polypeptide, an aggregate spider silk polypeptide, an aciniform spider silk polypeptide or a pyriform spider silk polypeptide. A “repetitive unit” refers to a region which corresponds in amino acid sequence to a region that comprises or consists of at least one peptide motif (e g. AAAAAA or GPGQQ) that repetitively occurs within a naturally occurring silk polypeptide (e.g. MaSpI, ADF-3, ADF-4, or Flag) (i.e. identical amino acid sequence) or to an amino acid sequence substantially similar thereto (i.e. variational amino acid sequence). A “repetitive unit” having an amino acid sequence which is “substantially similar” to a corresponding amino acid sequence within a naturally occurring silk polypeptide (i.e. wild-ty pe repetitive unit) is also similar with respect to its properties, e.g. a silk protein comprising the “substantially similar repetitive unit” is still insoluble and retains its insolubility. A “repetitive unit” having an amino acid sequence which is “identical” to the amino acid sequence of a naturally occurring silk polypeptide, for example, can be a portion of a silk polypeptide corresponding to one or more peptide motifs of MaSpI, MaSpII. ADF-3 and / or ADF-4. A “repetitive unit” having an amino acid sequence which is “substantially similar” to the amino acid sequence of a naturally occurring silk polypeptide, for example, can be a portion of a silk polypeptide corresponding to one or more peptide motifs of MaSpI, MaSpII, ADF-3 and / or ADF-4, but having one or more amino acid substitution at specific amino acid positions.As used herein, the term ‘‘consensus peptide sequence” refers to an amino acid sequence which contains amino acids which frequently occur in a certain position (e.g. “G”) and wherein, other amino acids which are not further determined are replaced by the place holder '‘X”. In some embodiments, the consensus sequence is at least one of (i) GPGXX, wherein X is an amino acid selected from A, S, G, Y, P and Q; (ii) GGX, wherein X is an amino acid selected from Y, P, R, S, A, T, N and Q, preferably Y, P and Q; (iii) Ax, wherein x is an integer from 5 to 10.The consensus peptide sequences GPGXX and GGX, i.e. glycine rich motifs, provide flexibility to the silk polypeptide and thus, to the thread formed from the silk protein containing said motifs. In detail, the iterated GPGXX motif forms turn spiral structures, which imparts elasticity to the silk polypeptide. Major ampullate and flagelliform silks both have a GPGXX motif. The iterated GGX motif is associated with a helical structure having three amino acids per turn and is found in most spider silks. The GGX motif may provide additional elastic properties to the silk. The iterated poly alanine Ax (peptide) motif forms a crystalline β-sheet structure that provides strength to the silk polypeptide, as described for example in WO 03 / 057727. In some embodiments, the recombinant spider silk protein in this disclosure comprises two identical repetitive units each comprising at least one, preferably one, amino acid sequence selected from the group consisting of: GGRPSDTYG and GGRPSSSYG derived from Resilin. Resilin is an elastomeric protein found in most arthropods that provides low stiffness and high strength.As used herein, “non-repetitive units” refers to an amino acid sequence which is “substantially similar” to a corresponding non-repetitive (carboxy terminal) amino acid sequence within a naturally occurring dragline polypeptide (i.e. wild-type non- repetitive (carboxy terminal) unit), preferably within ADF-3, ADF-4, NR3, NR4 of the spider Araneus diadematus, which is also described in U.S. Pat. No. 9,217,017, which is incorporated by reference herein in its entirety, C 16 peptide (sp...

Claims

CLAIMS1. A formulation comprising a polysaccharide component and a silk fibroin component, wherein the silk fibroin component comprises a plurality of fibroin heavy chain peptides or protein fragments, a plurality of fibroin light chain peptides or protein fragments, and / or a plurality of fibrohexamerin (p25) plurality of peptides or protein fragments, each peptide or protein fragment comprising a plurality of amino acids selected from M, R, V, K, T, F, I, L, C, A, Q, Y, N, D, E, G, S, H, P, and W, wherein at one or more independent occurrences at least one amino acid in a peptide or protein fragment is modified, substituted, or replaced.

2. The formulation of claim 1, wherein a modification, substitution, and / or replacement is selected from an asparagine to aspartic acid modification, substitution, and / or replacement, a glutamine to glutamic acid modification, substitution, and / or replacement, and a methionine to methionine oxide modification, substitution, and / or replacement.

3. The formulation of claim 1 or 2, wherein an amino acid modification, substitution, and / or replacement in a fibroin heavy chain peptide or protein fragment is at Q58, M64, N68. N70. N77, M80. N93, M103, Q125. N132, Q139. Q275, N4191, Q5216, and / or N5262, wherein each amino acid position is defined relative to the full sequence of the fibroin heavy chain.

4. The formulation of claim 1 or 2, wherein an amino acid modification, substitution, and / or replacement in a fibroin light chain peptide or protein fragment is at N23, Q24, N28, M69, N105, N108, N118, N136, N138, Q149, N186, N200, Q202, N204, N240, N248, and / or Q255, wherein each amino acid position is defined relative to the full sequence of the fibroin light chain.

5. The formulation of claim 1 or 2, wherein an amino acid modification, substitution, and / or replacement in a fibrohexamerin (p25) chain peptide or protein fragment is at Q62, N93, M120, N149, N172, N174, and / or N202, wherein each amino acid position is defined relative to the full sequence of the fibrohexamerin (p25) chain.

6. The formulation of any one of claims 1 to 5, the plurality of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 1 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, from between about 160 kDa and about180 kDa, from between about 180 kDa and about 200 kDa, from between about 200 kDa and about 250 kDa, or from between about 250 kDa and about 300 kDa.

7. The formulation of claim 6, the plurality of peptides or protein fragments having a polydispersity between 1 and about 3.

8. The formulation of any one of claims 1 to 5, the plurality of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 1 kDa and about 20 kDa. from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, or from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 250 kDa, and a poly dispersity between 1 and about 1.7, or between about 1.7 and about 2.5.

9. The formulation of any one of claims 1 to 5, the plurality of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 10 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa. from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, or from between about 160 kDa and about 180 kDa, and a poly dispersity between 1 and about 1.1, between about 1.1 and about 1.2, or between about 1.2 and about 2.4.

10. The formulation of any one of claims 1 to 5, the plurality' of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa, from betw een about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 220 kDa, and a poly dispersity between 1 and about 1.7, or between about 1.7 and about 2.5.

11. The formulation of any one of claims 1 to 5, the composition comprising a plurality of peptides or protein fragments fractions.

12. The formulation of claim 11, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 10 kDa and about 20 kDa. from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, or from between about 120 kDa and about 140 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.

2. between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.

3. or between about 2.3 and about 2.4.

13. The formulation of claim 11, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 60 kDa and about 80 kDa. from between about 80 kDa and about 100 kDa. or from between about 100 kDa and about 120 kDa, and a poly dispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.

8. betweenabout 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about2.

2. between about 2.2 and about 2.3, or between about 2.3 and about 2.4.

14. The formulation of claim 1 1, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 10 kDa and about 20 kDa, from between about 20 kDa and about 40 kDa, from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, or from between about 100 kDa and about 110 kDa, and a poly dispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.4.

15. The formulation of claim 11. the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, or from between about 120 kDa and about 140 kDa. and a poly dispersity between 1 and about 1.

1. between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2. 1 and about 2.2, between about 2.2 and about2.3, or between about 2.3 and about 2.4.

16. The formulation of claim 11, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 20 kDa and about 40 kDa. or from between about 40 kDa and about 60 kDa, and a poly dispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9and about 2, between about 2. 1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.4.

17. The formulation of claim 1 1, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa. from between about 100 kDa and about120 kDa, from between about 120 kDa and about 140 kDa, from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, or from between about 200 kDa and about 210 kDa. and a poly dispersity between 1 and about 1.

1. between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2. 1 and about 2.2, between about 2.2 and about 2.

3. or between about 2.3 and about 2.4.

18. The formulation of claim 11, the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 40 kDa and about 60 kDa, from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, or from between about 100 kDa and about 110 kDa, and a poly dispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.

7. between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2. 1 and about 2.2, between about 2.2 and about 2.3, or between about 2.3 and about 2.4.

19. The formulation of claim 11. the plurality of peptides or protein fragments in a fraction having a weight average molecular weight (Mw) selected from between about 60 kDa and about 80 kDa, from between about 80 kDa and about 100 kDa, from between about 100 kDa and about 120 kDa, from between about 120 kDa and about 140 kDa. from between about 140 kDa and about 160 kDa, from between about 160 kDa and about 180 kDa, from between about 180 kDa and about 200 kDa, orfrom between about 200 kDa and about 210 kDa, and a poly dispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.

3. between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2.1 and about 2.

2. between about 2.2 and about 2.3, or between about 2.3 and about 2.4.

20. The formulation of any one of claims 1 to 5, the plurality of peptides or protein fragments having a weight average molecular weight (Mw) selected from between about 10 kDa and about 30 kDa, from between about 30 kDa and about 50 kDa, or from between about 50 kDa and about 70 kDa, and a polydispersity between 1 and about 1.1, between about 1.1 and about 1.2, between about 1.2 and about 1.3, between about 1.3 and about 1.4, between about 1.4 and about 1.5, between about 1.5 and about 1.6, between about 1.6 and about 1.7, between about 1.7 and about 1.8, between about 1.8 and about 1.9, between about 1.9 and about 2, between about 2. 1 and about 2.2, between about 2.2 and about 2.

3. or between about 2.3 and about 2.4.

21. The formulation of any one of claims 1 to 20, wherein the polysaccharide component comprises a gum selected form xanthan gum, sclerotium gum, and guar gum.

22. The formulation of any one of claims 1 to 20, wherein the polysaccharide component comprises one or more of microcrystalline cellulose, cellulose gum, carrageenan gum, hydroxyethyl cellulose, carrageenan, pullulan, hyaluronan, and / or sodium alginate.

23. The formulation of any one of claims 1 to 22, wherein the (w / w) ratio of the polysaccharide component to the silk fibroin component is selected from about 1 : 10, about 1:9, about 1 :8, about 1 :7, about 1:6, about 1:5, about 1 :4, about 1 :3, about 1 :2, about 1 : 1, about 2:1, about 3: 1, about 4: 1, about 5: 1, about 6: 1, about 7: 1, about 8: 1, about 9: 1, and about 10:1.

24. The formulation of any one of claims 1 to 23, wherein polysaccharide component and the silk fibroin component are formulated as a fibroin proteoglycan complex.

25. The formulation of claim 24, wherein the fibroin proteoglycan complex comprises a plurality of non-covalent interactions, and / or a plurality of covalent bonds.

26. The formulation of any one of claims 1 to 25, wherein the formulation is formulated as an emulsifier formulation, wherein the emulsifier formulation can be liquid or substantially solid.

27. The formulation of any one of claims 1 to 25, wherein the formulation is formulated as a hydrogel.

28. The formulation of any one of claims 1 to 25, wherein the formulation is formulated as a powder.

29. The formulation of any one of claims 1 to 25, wherein the formulation is formulated into a pellet form.

30. The formulation of any one of claims 1 to 25, wherein the formulation is formulated as a lyophilized formulation.

31. The formulation of any one of claims 1 to 25, wherein the formulation is formulated as a hair care product.

32. The formulation of any one of claims 1 to 25, wherein the formulation is formulated as a shampoo, a hair conditioner, or skin cream.

33. The formulation of any one of claims 1 to 32, further comprising one or more of a co-surfactant and / or a co-emulsifier, a preservative, a rheology modifier, a humectant, an alcohol, an ester, a chelating agent, an anti-bacterial, a preservative, a thickening agent, a nanoclay, and / or a stabilizer agent.

34. The formulation of any one of claims 1 to 32, further comprising one or more of an anionic surfactant, a non-sulfate anionic surfactant, an amphoteric surfactant, a non-ionic surfactant, a cationic surfactant, a cationic thickener, and / or a poly quatemi urn.

35. The formulation of any one of claims 1 to 32, further comprising one or more of sodium laureth sulfate, sodium lauryl sarcosinate, cocamidopropyl betaine, capryl glucoside, caprylyl / capryl glucoside, coco-caprylate / caprate, behentrimonium chloride, cetrimonium chloride, stearamidopropyl dimethylamine, hydroxy propyltrimonium chloride, and / or polyquatemium-7, and / or di heptyl succinate.

36. The formulation of any one of claims 1 to 32, further comprising one or more of a carbomer, a capiyloyl glycerin / sebacic acid copolymer, and / or an acrylate copolymer.

37. The formulation of any one of claims 1 to 32, further comprising an alcohol component.

38. The formulation of any one of claims 1 to 32, further comprising one or more of phenethyl alcohol, pentylene glycol, propanediol, glycerin, behenyl alcohol, ce teary I alcohol, a C14-22 alcohol, pentylene glycol, and / or cetyl alcohol.

39. The formulation of any one of claims 1 to 32, further comprising one or more of glycolic acid, citric acid, mandelic acid, aspartic acid, gluconolactone, and / or lactobionic acid.

40. The formulation of any one of claims 1 to 32, further comprising a vegetable oil. a mineral oil, and / or a silicone oil.

41. The formulation of any one of claims 1 to 32, further comprising one or more of jojoba oil, argan oil, almond oil, coconut oil, squalane, mineral oil, dimethicone, olive oil, avocado oil, grape seed oil, fractionated coconut oil. undecane, tridecane, and / or cyclopentasiloxane.

42. The formulation of any one of claims 1 to 32, further comprising one or more of shea butter, cocoa butter, beeswax, and / or sunflower wax.

43. The formulation of any one of claims 1 to 32, further comprising one or more of tetrasodium glutamate diacetate, polyglyceryl-3 stearate, glycery l stearate SE, jojoba esters, sodium stearoyl glutamate, squalane, diheptyl succinate, capryloyl glycerin / sebacic acid copolymer, polyglyceryl-6 distearate, behenoyl stearic acid, myristyl myristate, C18-21 alkane, sodium cocoyl glycinate, lauryl glucoside, C12-20 alkyl glucoside, dicaprylyl carbonate, sucrose laurate, sucrose stearate, and / or cocamidopropyl betaine.

44. The formulation of any one of claims 1 to 32, further comprising one or more of Melia Azadirachta Leaf Extract, Melia Azadirachta Flower Extract, Corallina Officinalis Extract, Coccinia Indica Fruit Extract, Solanum Melongena (Eggplant) Fruit Extract. Aloe Barbadensis Flower Extract, Simmondsia Chinensis (Jojoba) Seed Oil. Ocimum Sanctum Leaf Extract. Ocimum Basilicum (Basil) Flower / Leaf Extract, and / or Curcuma Longa (Tumeric) Root Extract.

45. The formulation of any one of claims 31 to 44. wherein the formulation is formulated as an emulsion.

46. The formulation of any one of claims 31 to 44, wherein the formulation is formulated as a cream, paste, wax, and / or a moisturizer.

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