Cosmetic composition for preventing scalp heat aging and hair loss containing extracts of anastatica hierochuntica, opuntia ficus-indica, and mesembryanthemum crystallinum

Abstract

The present invention relates to a cosmetic composition for improving skin condition or scalp condition, comprising a mixture of an extract of Anastatica hierochuntica, an extract of Opuntia ficus-indica, and an extract of Mesembryanthemum crystallinum. The cosmetic composition can have a scalp heat aging prevention effect, an anti-inflammatory effect, and a hair loss prevention effect.

Description

Cosmetic composition for preventing scalp heat aging and hair loss containing extracts of resurrection plant, prickly pear, and ice plant

[0001] This invention relates to a cosmetic composition having skin condition improvement effects and scalp condition improvement effects comprising a mixture of plant extracts.

[0002] The recent continuous rise in average temperatures due to global warming is increasing interest in skin health. Skin repeatedly exposed to heat suffers from problems such as reduced elasticity, increased wrinkles, and moisture loss. This weakens the skin barrier function, creating an environment that increases sensitivity to external stimuli and promotes inflammatory responses. Consequently, skin redness occurs easily, and weakened immune function increases the likelihood of inflammation.

[0003] The scalp also faces similar problems when exposed to high temperatures. Vasodilation and increased blood flow caused by heat stimulate the sebaceous glands, leading to excessive sebum secretion. This results in clogged pores and inflammation, which can lead to scalp conditions such as seborrheic dermatitis. Furthermore, changes in skin protein structures and damage to collagen and elastin fibers caused by heat can accelerate scalp aging and ultimately lead to hair loss.

[0004] Against this backdrop, the importance of research and development on skin care products and methods capable of effectively resolving heat-related problems of the scalp and skin is being highlighted.

[0005] Accordingly, the inventors recognized the need for scalp and skin heat care and sought to develop a cosmetic composition for alleviating heat-induced inflammation and preventing hair loss in a scalp heat aging model. As a result, the present invention was completed by confirming that a mixture of specific plant extracts exhibits excellent effects in preventing scalp heat aging, anti-inflammation, and hair loss.

[0006] One aspect provides a mixture of extracts of resurrection plant (Anastatica hierochuntica), prickly pear (Opuntia ficus-indica), and ice plant (Mesembryanthemum crystallinum).

[0007] Another aspect is to provide a cosmetic composition for improving skin condition or scalp condition, comprising the above mixture.

[0008] Another aspect is to provide a topical skin composition for improving skin condition or scalp condition, comprising the above mixture.

[0009] Another aspect is to provide a health functional food composition for improving skin condition or scalp condition, comprising the above mixture.

[0010] Another aspect is to provide a pharmaceutical composition for the treatment or prevention of skin inflammatory diseases or hair loss, comprising the above mixture.

[0011] Another aspect provides for the use of a mixture of resurrection plant (Anastatica hierochuntica) extract, prickly pear (Opuntia ficus-indica) extract, and ice plant (Mesembryanthemum crystallinum) extract for improving skin or scalp condition.

[0012] Another aspect provides for the use of a mixture of resurrection plant (Anastatica hierochuntica) extract, prickly pear (Opuntia ficus-indica) extract, and ice plant (Mesembryanthemum crystallinum) extract for the treatment or prevention of skin inflammatory diseases or hair loss.

[0013] Another aspect provides the use of a mixture of resurrection plant (Anastatica hierochuntica) extract, prickly pear (Opuntia ficus-indica) extract, and ice plant (Mesembryanthemum crystallinum) extract for the preparation of cosmetic compositions for improving skin condition or scalp condition.

[0014] Another aspect provides a method for improving skin or scalp condition, comprising the step of administering to an individual a mixture of effective amounts of resurrection plant (Anastatica hierochuntica) extract, prickly pear (Opuntia ficus-indica) extract, and ice plant (Mesembryanthemum crystallinum) extract.

[0015] Another aspect provides a method for treating or preventing skin inflammatory diseases or hair loss, comprising the step of administering to an individual a mixture of effective amounts of Anastatica hierochuntica extract, Opuntia ficus-indica extract, and Mesembryanthemum crystallinum extract.

[0016] One aspect provides a mixture of resurrection plant extract, prickly pear extract, and ice plant extract.

[0017] The term "resurrection plant" (Anastatica hierochuntica, Myrothamnus flabellifolia, Selaginella lepidophylla, Lycopodium lepidophyllum) refers to a plant capable of surviving in extremely dry environments. It possesses the ability to recover and survive upon receiving water, even in extreme dry conditions where it loses most of its moisture. It is characterized by leaves that fold and curl when dry and unfold upon receiving moisture. Due to these adaptive properties for dry environments, it is attracting attention as an ingredient in skincare products with moisturizing and antioxidant effects.

[0018] The term "Opuntia ficus-indica" refers to a cactus native to Mexico that is currently cultivated in various regions around the world, characterized by its flat, palm-shaped stems. It is also known as the "prickly pear," "fan cactus," or "Opuntia ficus-indica var. sarboten," and produces fruits in colors such as yellow, red, and purple. Due to its excellent water retention and adaptability to drought, it is utilized as an ingredient in skincare products with moisturizing, antioxidant, and soothing effects.

[0019] The term "ice plant (Mesembryanthemum crystallinum, glacial ficoide)," also known as "ice plant" or "crystal ice plant," refers to an annual or biennial herbaceous plant belonging to the Asteraceae family. Native to South Africa, the Mediterranean coast, and Australia, it is characterized by thick, succulent leaves with transparent, teardrop-shaped protrusions on the surface that resemble ice crystals. These leaf protrusions accumulate salt and store water, providing strong resistance to salinity and drought. Furthermore, as it performs CAM photosynthesis, it can survive even in extreme environments. Containing antioxidants, flavonoids, and various minerals, it is utilized as an ingredient in skincare products for its moisturizing, antioxidant, anti-inflammatory, and skin regeneration effects.

[0020] The term "extract" refers to an active ingredient isolated from a natural product. The extract may be obtained by an extraction process using water, an organic solvent, or a mixture thereof, and includes the extract liquid, the dried powder thereof, or any form formulated using the extract. It includes the extract itself and all formulations of extracts that can be formed using the extract, such as the extract liquid obtained by the extraction treatment of a natural product, a diluted or concentrated solution of the extract liquid, a dried product obtained by drying the extract liquid, a modified or purified product of the extract liquid, a fraction, or a mixture thereof.

[0021] The above extract may be prepared using general manufacturing methods known in the art and is not particularly limited. For example, it may be prepared by sonication extraction, supercritical extraction, vacuum extraction, reflux extraction, hot-water extraction, autoclave extraction, high-pressure extraction, enzyme extraction, or by a known method using an extraction solvent, and specifically, it may be prepared by sonication extraction, but is not limited thereto.

[0022] The above extract may be a plant extract, specifically a resurrection plant extract, a prickly pear extract, or an ice plant extract.

[0023] The term "ultrasonic extraction method" refers to an extraction method that utilizes acoustic cavitation generated by irradiating an extraction solvent with ultrasound (sound waves having a frequency of 20 kHz or higher). Specifically, it is an extraction method that utilizes the phenomenon in which strong shock waves and extreme local temperature and pressure conditions generated during the process in which microbubbles created by ultrasound grow and then explosively collapse destroy plant cell walls, thereby rapidly releasing useful components from within the cell into the solvent. Compared to conventional hot water extraction or solvent extraction methods, it has the advantage of shortening extraction time and improving extraction efficiency.

[0024] The ultrasound used in the above ultrasound extraction method may be 16 kHz to 100 kHz, 16 kHz to 500 kHz, 16 kHz to 200 kHz, 16 kHz to 100 kHz, 20 kHz to 100 kHz, 20 kHz to 80 kHz, 20 kHz to 70 kHz, or 20 kHz to 60 kHz.

[0025] The above ultrasonic extraction method may use one or more extraction solvents selected from the group consisting of distilled water, ethanol, methanol, butanol, acetone, ethyl acetate, hexane, propanol, butylene glycol, and propylene glycol.

[0026] In the above extract, the amount of extraction solvent relative to the weight of the raw material may be 1 to 100 times, 5 to 50 times, 5 to 40 times, 5 to 40 times, 5 to 35 times, 10 to 30 times, 15 to 25 times, or 20 times.

[0027] The above extract may be extracted at 0°C to 100°C, 5°C to 100°C, 10°C to 90°C, 20°C to 80°C, 30°C to 70°C, 35°C to 65°C, or 40°C to 60°C.

[0028] The above extract may be extracted for 6 to 150 hours, 12 to 144 hours, 24 to 132 hours, 48 ​​to 108 hours, 52 to 104 hours, 44 to 100 hours, 48 ​​to 96 hours, 52 to 92 hours, 56 to 88 hours, 60 to 84 hours, 64 to 80 hours, 68 to 76 hours, or 72 hours.

[0029] The amount of solvent and extraction time can be appropriately selected and increased. In the above extraction methods, the extraction process can be repeated two or more times as needed. The extract obtained by filtering the above extract may be prepared by concentrating the filtrate under reduced pressure, or the extract obtained after filtration may be freeze-dried or vacuum-dried to form a powder.

[0030] The above-mentioned resurrection plant extract, prickly pear extract, or ice plant extract may be extracted by ultrasonic extraction.

[0031] In one embodiment, the mixture may be a mixture of resurrection plant extract, prickly pear extract, and ice plant extract.

[0032] The above mixture may be a mixture of resurrection plant extract, prickly pear extract, and ice plant extract in a weight ratio of 1:0.1 to 10:0.1 to 10, 1:0.4 to 6:0.4 to 6, 1:0.6 to 4:0.6 to 4, 1:0.8 to 2:0.8 to 2, 1:0.8 to 1.5, 0.8 to 1.5, 1:0.8 to 1.2:0.8 to 1.2, more specifically 1:1:1.

[0033] The above mixture may contain resurrection plant extract in an amount of 10 to 90 weight%, 15 to 80 weight%, 20 to 70 weight%, 22 to 60 weight%, 23 to 50 weight%, 25 to 40 weight%, 27 to 38 weight%, 29 to 36 weight%, 31 to 35 weight%, or 33.3 weight%.

[0034] The above mixture may contain prickly pear extract in an amount of 10 to 90 weight%, 15 to 80 weight%, 20 to 70 weight%, 22 to 60 weight%, 23 to 50 weight%, 25 to 40 weight%, 27 to 38 weight%, 29 to 36 weight%, 31 to 35 weight%, or 33.3 weight%.

[0035] The above mixture may contain ice plant extract in an amount of 10 to 90 weight%, 15 to 80 weight%, 20 to 70 weight%, 22 to 60 weight%, 23 to 50 weight%, 25 to 40 weight%, 27 to 38 weight%, 29 to 36 weight%, 31 to 35 weight%, or 33.3 weight%.

[0036] Specifically, the above mixture may be a mixture of 25 to 40 weight% resurrection plant extract, 25 to 40 weight% prickly pear extract, and 25 to 40 weight% ice plant extract.

[0037] The above mixture may be a mixture of resurrection plant extract, prickly pear extract, and ice plant extract at room temperature, for example, at -10℃ to 50℃, 0℃ to 40℃, 10℃ to 30℃, 20℃ to 30℃, or 25℃.

[0038] In one embodiment, it was confirmed that the mixture of the resurrection plant extract, the prickly pear extract, and the ice plant extract showed significantly superior effects in preventing scalp heat aging, anti-inflammatory effects, and hair loss prevention effects compared to the use of the resurrection plant extract, the prickly pear extract, or the ice plant extract alone (Experimental Examples 1 to 3).

[0039] Another aspect provides a composition comprising the above mixture. In the composition, any terms or elements mentioned that are identical to those already mentioned are as described above.

[0040] A composition containing the above mixture may have effects such as preventing scalp heat aging, anti-inflammatory effects, and preventing hair loss.

[0041] In one embodiment, the composition may be for improving skin condition or scalp condition.

[0042] In one embodiment, the improvement of the skin condition may mean preventing skin aging, improving skin wrinkles, improving skin elasticity, strengthening the skin barrier, alleviating skin heat sensation, anti-inflammatory, antioxidant, anti-acne, etc., and specifically may mean preventing skin aging, alleviating skin heat sensation, or anti-inflammatory, and more specifically may mean anti-inflammatory, but is not limited thereto.

[0043] In one embodiment, the improvement of the scalp condition may mean preventing hair loss, promoting hair growth, protecting hair, promoting hair growth, increasing hair thickness, preventing scalp aging, preventing scalp heat aging, alleviating scalp heat sensation, suppressing scalp inflammation, etc. Specifically, it may mean protecting hair, preventing hair loss, preventing scalp heat aging, alleviating scalp heat sensation, or suppressing scalp inflammation, and more specifically, it may mean preventing scalp heat aging, anti-inflammation, or preventing hair loss, but is not limited thereto.

[0044] The term "improvement of skin condition" refers to all actions that enhance the health and appearance of the skin, including improving moisture balance, increasing elasticity, improving skin texture, reducing pigmentation, whitening the skin, strengthening the skin barrier, reducing inflammation, regulating sebum secretion, providing antioxidant effects, improving skin blood circulation, promoting skin regeneration, healing skin wounds, alleviating skin heat, and preventing skin aging. Specifically, the improvement of skin condition may refer to, but is not limited to, preventing skin aging, alleviating skin heat, or anti-inflammatory effects.

[0045] The term "anti-inflammatory" refers to any action that suppresses inflammation.

[0046] The term "improvement of scalp condition" refers to all actions that improve the health and appearance of the scalp and hair, including normalization of the hair cycle, strengthening of hair roots, prevention of hair loss, improvement of the scalp environment, regulation of scalp hormone balance, reduction of scalp inflammation, alleviation of scalp heat sensation, prevention of scalp heat aging, prevention of oxidative stress, strengthening of hair protein, supplying nutrition to the scalp, regulation of scalp sebum, promotion of hair growth, improvement of hair thickness, stress management, etc. The above-mentioned improvement of scalp condition may mean hair protection, prevention of hair loss, prevention of scalp heat aging, alleviation of scalp heat sensation, or suppression of scalp inflammation, but is not limited thereto.

[0047] The term "prevention of scalp thermal aging" refers to all actions that prevent and inhibit scalp damage and aging caused by external heat sources or exposure to ultraviolet rays. Since scalp thermal aging can lead to hair loss, inflammation, and a decline in scalp function, proper scalp care is essential.

[0048] The term "hair loss prevention" refers to all actions that prevent abnormal hair loss and maintain healthy hair growth.

[0049] In one embodiment, the composition may reduce the mRNA expression of the heat-sensing factor TRPV1 / β-actin. Accordingly, the composition may have an effect of preventing scalp heat aging or alleviating scalp heat sensation.

[0050] In one embodiment, the composition may reduce the mRNA expression of the inflammatory factor IL-1b / β-actin. Accordingly, the composition may have an anti-inflammatory effect or an effect that inhibits scalp inflammation.

[0051] In one embodiment, the composition may reduce the mRNA expression of DKK-1 / β-actin, a hair loss factor. Accordingly, the composition may have a hair protective effect or a hair loss prevention effect.

[0052] In one embodiment, it was confirmed that a 1:1:1 mixture of resurrection plant extract, prickly pear extract, and ice plant extract prepared by the method according to one aspect of the above was significantly superior to the use of resurrection plant extract, prickly pear extract, or ice plant extract alone in terms of preventing scalp heat aging, anti-inflammatory effect, and hair loss prevention effect (Experimental Examples 1 to 3).

[0053] In one embodiment, the composition may be a cosmetic composition.

[0054] The above cosmetic composition may be prepared in a formulation comprising a lotion (skin lotion), skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, eye cream, hand cream, foundation, essence, nourishing essence, eye essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cream, body cleanser, suspension, gel, powder, paste, mask pack or sheet, or aerosol composition. Compositions of such formulations may be prepared according to methods conventional in the field.

[0055] The ingredients included in the above cosmetic composition may include ingredients commonly used in cosmetic compositions in addition to the mixture of resurrection plant extract, prickly pear extract, and ice plant extract as active ingredients, and may include, for example, conventional auxiliary agents and carriers such as stabilizers, solubilizers, vitamins, pigments, and fragrances.

[0056] In one embodiment, the composition may be a skin external application composition.

[0057] In this specification, the term "external skin preparation" is a concept that generally encompasses all compositions used externally, and may refer to a wide range of products that can be applied to cosmetic compositions containing various cosmetics such as basic cosmetics, makeup cosmetics, hair cosmetics, shaving cosmetics, or various pharmaceuticals or quasi-pharmaceuticals such as ointments. For example, the above quasi-pharmaceuticals may be disinfectants, shower foams, mouthwash, wet wipes, detergent soaps, hand washes, humidifier fillers, masks, ointments, coating agents, or filter fillers.

[0058] The above-mentioned topical skin composition may additionally contain, in addition to the active ingredient, ingredients commonly incorporated in topical preparations according to the intended use and the properties of the topical composition, specifically moisturizers, UV absorbers, vitamins, animal and plant extracts, digestive agents, whitening agents, vasodilators, astringents, cooling agents, hormones, etc.

[0059] The formulation of the above-mentioned external skin composition may take an appropriate form depending on the intended use and the properties of the external composition, and specifically may be an aqueous solution type, a solubilization type, an emulsion type, a liquid type, a gel type, a paste type, an ointment type, an aerosol type, a water-oil two-layer type, or a water-oil-powder three-layer type, but the formulation and form of the external agent of the present invention are not limited by the above-mentioned formulation.

[0060] In addition, the above-mentioned topical skin preparation may further include a mechanism necessary to penetrate or transfer the active ingredient into the skin tissue.

[0061] In one embodiment, the composition may be a pharmaceutical composition.

[0062] The above pharmaceutical composition may be a pharmaceutical composition for the treatment or prevention of skin inflammatory diseases or hair loss.

[0063] The above-mentioned skin inflammatory disease includes all inflammatory diseases that may occur on the skin regardless of the cause. For example, it may include skin wounds, dermatitis, pruritus, eczematous skin disease, dry eczema, erythema, urticaria, psoriasis, drug rash, acne, inflammatory scalp disease, seborrheic dermatitis or scalp dermatitis, atopic dermatitis, contact dermatitis, or stress-induced inflammation.

[0064] The term "treatment" refers to any act of improving or beneficially altering the symptoms of a skin inflammatory disease through the administration of the above-mentioned composition.

[0065] The term "prevention" refers to any act in which the possibility of developing skin inflammatory diseases is suppressed or delayed by the administration of the above-mentioned composition.

[0066] The above pharmaceutical composition may be formulated and used in the form of tablets, pills, powders, granules, capsules, suspensions, oral liquids, emulsions, or syrups, as well as external preparations, suppositories, or injectable solutions, according to conventional methods. Specifically, when formulating, it may be prepared using diluents or excipients such as commonly used fillers, weighting agents, binders, wetting agents, disintegrants, and surfactants.

[0067] The above pharmaceutical composition may be administered orally or parenterally (e.g., intravenously, subcutaneously, intraperitoneally, or topically) depending on the intended method, and the dosage may be appropriately selected by a person skilled in the art, although it depends on the patient's condition and body weight, the severity of the disease, the form of the drug, the route of administration, and the time.

[0068] In one embodiment, the composition may be a health functional food composition.

[0069] The above-mentioned health functional food composition may be used alone or in combination with other foods or food ingredients, and may be used appropriately according to conventional methods. The amount of the active ingredient mixture may be appropriately determined according to the purpose of use (prevention, health, or therapeutic treatment). There are no special restrictions on the types of the above-mentioned health functional food. Among the types of health functional foods, the beverage composition may contain various flavoring agents or natural carbohydrates as additional ingredients, as in ordinary beverages. The above-mentioned natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As sweeteners, natural sweeteners such as taumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used. The above health functional food composition may also contain nutritional agents, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, or combinations thereof. The above health functional food composition may also contain fruit pulp for the production of natural fruit juice, fruit juice beverages, vegetable beverages, or combinations thereof.

[0070] Another aspect provides the use of a mixture of resurrection plant (Anastatica hierochuntica) extract, prickly pear (Opuntia ficus-indica) extract, and ice plant (Mesembryanthemum crystallinum) extract for improving skin or scalp condition.

[0071] Another aspect provides the use of a mixture of resurrection plant (Anastatica hierochuntica) extract, prickly pear (Opuntia ficus-indica) extract, and ice plant (Mesembryanthemum crystallinum) extract for the treatment or prevention of skin inflammatory diseases or hair loss.

[0072] Another aspect provides the use of a mixture of resurrection plant (Anastatica hierochuntica) extract, prickly pear (Opuntia ficus-indica) extract, and ice plant (Mesembryanthemum crystallinum) extract for the preparation of cosmetic compositions for improving skin condition or scalp condition.

[0073] Another aspect provides a method for improving skin or scalp condition, comprising the step of administering to a subject an effective amount of a mixture of resurrection plant (Anastatica hierochuntica) extract, prickly pear (Opuntia ficus-indica) extract, and ice plant (Mesembryanthemum crystallinum) extract.

[0074] Another aspect provides a method for treating or preventing skin inflammatory diseases or hair loss, comprising the step of administering to an individual an effective amount of a mixture of resurrection plant (Anastatica hierochuntica) extract, prickly pear (Opuntia ficus-indica) extract, and ice plant (Mesembryanthemum crystallinum) extract.

[0075] In the above aspects, any terms or elements mentioned that are identical to those already mentioned are as stated above.

[0076] In the above aspects, "effective amount" means an amount of a composition containing a pharmacological component or active component in the composition in a sufficient quantity to provide a therapeutic, corrective, or preventive effect to an individual to whom the mixture or composition is to be administered. The effective amount may include the meanings of a "preventive effective amount," a "corrective effective amount," or a "therapeutic effective amount."

[0077] In the above aspects, "individual" may mean any individual requiring improvement of skin condition or scalp condition, or any individual requiring treatment or prevention of skin inflammatory disease or hair loss. The individual may include or exclude humans, and may include mammals, and the mammals may include, for example, humans, dogs, cats, rats, mice, rats, guinea pigs, horses, cattle, pigs, sheep, monkeys, chimpanzees, etc.

[0078] In the above aspects, "administration" means administering an effective amount of the mixture or composition directly to an individual to form it within the individual's body. The administration may be oral or parenteral, and may include transdermal administration, subcutaneous administration, or application to the skin. Suitable dosages of the mixture or composition may be prescribed differently depending on factors such as the formulation method, mode of administration, patient's age, body weight, sex, pathological condition, diet, time of administration, route of administration, excretion rate, and response sensitivity.

[0079] A mixture of resurrection plant extract, prickly pear extract, and ice plant extract according to one aspect has effects of preventing scalp heat aging, anti-inflammatory effects, and hair loss prevention effects, and can be applied in various ways such as cosmetic compositions for improving skin condition or scalp condition, external skin preparation compositions, pharmaceutical compositions, and health functional food compositions.

[0080] Figure 1 shows the mRNA expression levels of TRPV1 / β-actin to confirm the anti-thermal aging effects of the extract and mixture on the scalp.###p<0.001 vs. None.**p<0.01 vs. Heat.***p<0.001 vs. Heat.

[0081] Figure 2 shows the mRNA expression levels of IL-1b / β-actin to confirm the anti-inflammatory effects of the extracts and mixtures.###p<0.001 vs. None.*p<0.05 vs. Heat.***p<0.001 vs. Heat.

[0082] Figure 3 shows the mRNA expression levels of DKK-1 / β-actin to confirm the anti-hair loss effects of the extract and mixture.###p<0.001 vs. None.*p<0.05 vs. Heat.**p<0.01 vs. Heat.

[0083] The following examples will be explained in more detail. However, these examples are intended to illustrate one or more specific examples, and the scope of the present invention is not limited to these examples.

[0084] [Comparative Example]

[0085] Comparative Examples 1–3. Preparation of Resurrection Plant extract, Prickly Pear extract, and Ice Plant extract

[0086] A solvent (600g purified water, 400g butylene glycol) mixed with purified water and butylene glycol in a ratio of 6:4 was added to 50g of the raw material of the resurrection plant (Anastatica hierochuntica, Rose of Jericho) at a ratio of 20 times (1:20) relative to the original material, and then extracted for 72 hours by applying ultrasound of 20 to 60 kHz at a temperature range of 40 to 60℃ to prepare a resurrection plant extract (Comparative Example 1).

[0087] A solvent (600g purified water, 400g butylene glycol) mixed with purified water and butylene glycol in a ratio of 6:4 was added to 50g of Opuntia ficus-indica at a ratio of 20 times (1:20) relative to the raw Opuntia ficus-indica, and then extracted for 72 hours by applying ultrasound of 20 to 60kHz at a temperature range of 40 to 60℃ to prepare an Opuntia ficus-indica extract (Comparative Example 2).

[0088] For 50g of ice plant (Mesembryanthemum crystallinum, glacial ficoide), a solvent (600g purified water, 400g butylene glycol) mixed with purified water and butylene glycol in a ratio of 6:4 was added at a ratio of 20 times (1:20) relative to the raw ice plant, and then an ice plant extract was prepared by applying ultrasound of 20 to 60 kHz at a temperature range of 40 to 60℃ for 72 hours (Comparative Example 3).

[0089] [Example]

[0090] Example 1. Preparation of a mixture of resurrection plant extract, prickly pear extract, and ice plant extract

[0091] Example 1 was prepared by mixing resurrection plant extract (Comparative Example 1), prickly pear extract (Comparative Example 2), and ice plant extract (Comparative Example 3), prepared according to the above process, in a weight ratio of 1:1:1 (weight%) at room temperature (about 25℃).

[0092] [Experimental Example]

[0093] Experimental Example 1. Confirmation of the anti-thermal aging effect of the mixture on the scalp

[0094] To confirm the scalp heat aging prevention effect of Comparative Examples 1 to 3 and Example 1, an experiment was performed as follows.

[0095] Specifically, 3 x 10 human follicle dermal papilla cells (hDPCs) in a 6-well plate 5After seeding under cells / well conditions, the cells were cultured for 24 hours at 37°C, 5% CO2, and 95% humidity. Subsequently, the cells were washed once with DPBS and replaced with serum-free medium; 1 ppm of Example 1 and Comparative Examples 1, 2, and 3 were added, respectively, and the cells were further cultured for 24 hours at 37°C and 5% CO2. As a negative control, 37°C and 5% CO2 Standard cell culture conditions were used.

[0096] RNA was isolated from cells using the RNeasy Mini kit (Qiagen) and quantified using Nanodrop. cDNA was synthesized in an amplifier using 1 µg of RNA (C1000 Thermal Cycler, Bio-Rad, USA). Using the synthesized cDNA as a template, real-time polymerase chain reaction (PCR) was performed against the target gene TRPV1 on a real-time PCR machine (Step One Plus, Applied Biosystems, USA) by adding CyberGreen (SYBR Green supermix, Applied Biosystems, USA) along with the primers and cDNA. The expression level of the TRPV1 gene was finally analyzed after correction for the β-actin gene.

[0097] As a result, when the same concentration of active ingredient was applied as shown in Table 1 below, it was confirmed that Example 1 had a superior effect in reducing the expression of the heat-sensing factor TRPV1 compared to Comparative Examples 1 to 3 (Fig. 1).

[0098] [Table 1]

[0099]

[0100] Experimental Example 2. Confirmation of anti-inflammatory effect of the mixture

[0101] To confirm the anti-inflammatory effects of Comparative Examples 1 to 3 and Example 1, experiments were performed as follows.

[0102] Specifically, 3 x 10 human follicle dermal papilla cells (hDPCs) in a 6-well plate 5 After seeding under cells / well conditions, the cells were cultured for 24 hours at 37°C, 5% CO2, and 95% humidity. Subsequently, the cells were washed once with DPBS and replaced with serum-free medium; 1 ppm of Example 1 and Comparative Examples 1, 2, and 3 were added, respectively, and the cells were further cultured for 24 hours at 37°C and 5% CO2. As a negative control, 37°C and 5% CO2 Standard cell culture conditions were used.

[0103] RNA was isolated from cells using the RNeasy Mini kit (Qiagen) and quantified using Nanodrop. cDNA was synthesized in an amplifier using 1 µg of RNA (C1000 Thermal Cycler, Bio-Rad, USA). Using the synthesized cDNA as a template, real-time polymerase chain reaction (PCR) was performed against the target gene IL-1b on a real-time PCR machine (Step One Plus, Applied Biosystems, USA) by adding CyberGreen (SYBR Green supermix, Applied Biosystems, USA) along with the primers and cDNA. The expression level of the IL-1b gene was finally analyzed after correction for the β-actin gene.

[0104] As a result, when the same concentration of the active ingredient was applied as shown in Table 2 below, it was confirmed that the effect of reducing the expression of the inflammatory factor IL-1b in Example 1 was significantly superior compared to Comparative Examples 1 to 3 (Fig. 2).

[0105] [Table 2]

[0106]

[0107] Experimental Example 3. Confirmation of the hair loss prevention effect of the mixture

[0108] To confirm the hair loss prevention effects of Comparative Examples 1 to 3 and Example 1, an experiment was performed as follows.

[0109] Specifically, 3 x 10 human follicle dermal papilla cells (hDPCs) in a 6-well plate 5 After seeding under cells / well conditions, the cells were cultured for 24 hours at 37°C, 5% CO2, and 95% humidity. Subsequently, the cells were washed once with DPBS and replaced with serum-free medium; 1 ppm of Example 1 and Comparative Examples 1, 2, and 3 were added, respectively, and the cells were further cultured for 24 hours at 37°C and 5% CO2. As a negative control, 37°C and 5% CO2 Standard cell culture conditions were used.

[0110] RNA was isolated from cells using the RNeasy Mini kit (Qiagen) and quantified using Nanodrop. cDNA was synthesized in an amplifier using 1 µg of RNA (C1000 Thermal Cycler, Bio-Rad, USA). Using the synthesized cDNA as a template, real-time polymerase chain reaction (PCR) was performed against the target gene DKK-1 on a real-time PCR machine (Step One Plus, Applied Biosystems, USA) by adding CyberGreen (SYBR Green supermix, Applied Biosystems, USA) along with the primers and cDNA. The expression level of the DKK-1 gene was finally analyzed after correction for the β-actin gene.

[0111] As a result, when the same concentration of the active ingredient was applied as shown in Table 3 below, it was confirmed that Example 1 had a superior effect in reducing the expression of the hair loss factor DKK-1 compared to Comparative Examples 1 to 3 (Fig. 3).

[0112] [Table 3]

[0113]

[0114] In summary, when using the mixture of Example 1, which is a 1:1:1 mixture of resurrection plant extract, prickly pear extract, and ice plant extract, there is a significantly excellent effect of preventing scalp heat aging, anti-inflammatory effect, and hair loss prevention effect, so it can be used as a cosmetic composition for improving skin condition or scalp condition.

Claims

1. A cosmetic composition for improving skin condition or scalp condition, comprising a mixture of extracts of Anastatica hierochuntica (resurrection plant), Opuntia ficus-indica (prickly pear), and Mesembryanthemum crystallinum (ice plant).

2. A cosmetic composition for improving skin condition or scalp condition, wherein the resurrection plant extract, prickly pear extract, and ice plant extract are extracted by ultrasonic extraction.

3. A cosmetic composition for improving skin condition or scalp condition according to claim 1, wherein the mixture comprises 25 to 40 weight% of resurrection plant extract, 25 to 40 weight% of prickly pear extract, and 25 to 40 weight% of ice plant extract.

4. A cosmetic composition for improving skin condition or scalp condition, wherein the mixture of resurrection plant extract, prickly pear extract, and ice plant extract is mixed in a weight ratio of 1:0.1 to 10:0.1 to 10.

5. A cosmetic composition for improving skin condition or scalp condition, wherein the skin condition improvement according to Claim 1 is an anti-inflammatory.

6. A cosmetic composition for improving skin condition or scalp condition, wherein the improvement of scalp condition according to Claim 1 is prevention of scalp heat aging, anti-inflammation, and prevention of hair loss.

7. A topical skin composition for improving skin or scalp condition, comprising a mixture of extracts of *Anastatica hierochuntica*, *Opuntia ficus-indica*, and *Mesembryanthemum crystallinum*.

8. A health functional food composition for improving skin condition or scalp condition, comprising a mixture of resurrection plant (Anastatica hierochuntica) extract, prickly pear (Opuntia ficus-indica) extract, and ice plant (Mesembryanthemum crystallinum) extract.

9. A pharmaceutical composition for the treatment or prevention of skin inflammatory diseases or hair loss, comprising a mixture of extracts of *Anastatica hierochuntica*, *Opuntia ficus-indica*, and *Mesembryanthemum crystallinum*.

10. Use of a mixture of resurrection plant (Anastatica hierochuntica) extract, prickly pear (Opuntia ficus-indica) extract, and ice plant (Mesembryanthemum crystallinum) extract for improving skin condition or scalp condition.

11. Use of a mixture of extracts of resurrection plant (Anastatica hierochuntica), prickly pear (Opuntia ficus-indica), and ice plant (Mesembryanthemum crystallinum) for the treatment or prevention of skin inflammatory diseases or hair loss.

12. A method for improving skin or scalp condition, comprising the step of administering to a subject an effective amount of a mixture of resurrection plant (Anastatica hierochuntica) extract, prickly pear (Opuntia ficus-indica) extract, and ice plant (Mesembryanthemum crystallinum) extract.

13. A method for treating or preventing skin inflammatory diseases or hair loss, comprising the step of administering to an individual a mixture of effective amounts of Anastatica hierochuntica extract, Opuntia ficus-indica extract, and Mesembryanthemum crystallinum extract.

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