Method for optimizing nucleic acid library
By removing invalid sequences from single-cell and spatiotemporal omics libraries and utilizing primer and distinguishing element separation techniques, the problems of insufficient sequencing accuracy and throughput in existing technologies have been solved, enabling higher quality sequencing data and more comprehensive transcript information acquisition.
WO2026123312A1PCT designated stage Publication Date: 2026-06-18BGI RESEARCH HANGZHOU +1
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- BGI RESEARCH HANGZHOU
- Filing Date
- 2024-12-12
- Publication Date
- 2026-06-18
Smart Images
Figure CN2024138901_18062026_PF_FP_ABST
Abstract
The present application relates to a method for optimizing a nucleic acid library, and more specifically relates to a method for optimizing a single-cell library or spatio-temporal omics library. The nucleic acid library comprises a valid sequence and an invalid sequence. The valid sequence sequentially comprises a barcode domain, a target nucleic acid domain and a first universal primer binding domain. The invalid sequence comprises a first invalid sequence, wherein the first invalid sequence contains a barcode domain but does not contain a target nucleic acid domain or a first universal primer binding domain. The method comprises removing a first invalid sequence, and removing the first invalid sequence comprises the following steps: removing the first invalid sequence by using a first primer, wherein the first primer is partially or completely complementary to the first universal primer binding domain, and the first primer is labeled with a first distinguishing element.
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