Fermentation production method for threonine and use of inactivating or attenuating activity of nucleoside phosphorylase in microorganism in production of threonine

By reducing or inactivating nucleoside phosphorylase activity and removing feedback inhibition through genetic engineering, the threonine synthesis pathway was optimized, solving the problem of low yield caused by complex microbial metabolic networks and improving the production efficiency of L-threonine.

WO2026124123A1 Publication Date: 2026-06-18MEIHUA BIOTECH LANGFANG CO LTD
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Patent Information

Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
MEIHUA BIOTECH LANGFANG CO LTD
Filing Date
2025-11-17
Publication Date
2026-06-18

AI Technical Summary

⚠Technical Problem

In the existing technology for producing L-threonine using Corynebacterium glutamicum, the microbial metabolic network is complex, and there are negative effects caused by unknown mutations, which affect the yield and efficiency.

⚗Method used

By using genetic engineering methods to reduce or inactivate the activity of nucleoside phosphorylase and relieve the feedback inhibition of key genes lysC and hom in threonine synthesis, the gene thrABC in the threonine terminal synthesis pathway was expressed, and a recombinant microorganism was constructed to optimize its fermentation production of L-threonine.

🎯Benefits of technology

It significantly improved the yield and production efficiency of L-threonine, increased the concentration of threonine produced by fermentation, and enhanced the threonine synthesis capacity of microorganisms.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention relates to the technical field of microorganisms, and provides a fermentation production method for threonine and a use of inactivating or attenuating the activity of nucleoside phosphorylase in a microorganism in production of the threonine. It has been found in studies that inactivating or attenuating the activity of nucleoside phosphorylase in a microorganism can increase the yield of threonine produced by fermentation. Thus, a fermentation production method for L-threonine is provided, wherein a recombinant microorganism is used for fermentation production, the recombinant microorganism, compared with a starting strain, exhibits reduced or inactivated activity of nucleoside phosphorylase, and the amino acid sequence of the nucleoside phosphorylase is shown in any one of SEQ ID NOs: 28-30. A new and effective method is provided for production of threonine.
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