Nucleic acid detection composition based on differently modified templates, kit, and method
The nucleic acid detection system, which utilizes site-specific cleavage nucleases and universal primers for amplification, solves the problems of low sensitivity and complex operation in the detection of DNA methylation and RNA modification in existing technologies. It achieves highly sensitive and specific detection of trace amounts of nucleic acid, and provides an efficient detection method, especially at the single-cell level.
WO2026130213A1PCT designated stage Publication Date: 2026-06-25SHANGHAI SCI-TECH INNO CENTER FOR INFECTION & IMMUNITY
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- SHANGHAI SCI-TECH INNO CENTER FOR INFECTION & IMMUNITY
- Filing Date
- 2025-12-11
- Publication Date
- 2026-06-25
Smart Images
Figure CN2025141794_25062026_PF_FP_ABST
Abstract
Provided are a capture oligonucleotide, composition or kit for nucleic acid amplification, and a method. Specifically, the capture oligonucleotide comprises, from the 5' end to the 3' end, a universal sequence (3s) and a binding capture sequence (1s); wherein (1), the binding capture sequence (1s) is at least partially identical to the 5' end sequence of a target molecule or the binding capture sequence (1s) is at least partially identical to the 3' end sequence of a specific modification site on the target molecule; (2), the capture oligonucleotide further comprises a nucleic acid extension-blocking modification located at the 3' end of the binding capture sequence (1s); and optionally (3), the 3' end of the binding capture sequence (1s) has a nucleic acid analogue modification. Also provided are a composition or kit used for nucleic acid amplification detection, a detection system, and a method. The product and method can achieve nucleic acid detection with good specificity and high sensitivity.
Need to check novelty before this filing date? Find Prior Art