Two-component hair dye for non-oxidative hair dyeing

Abstract

The invention relates to a cosmetic product for non-oxidative dyeing of keratin fibers, in particular human hair, comprising two compositions that are separate from each other, wherein the first composition comprises at least one glycosylated dye or dye precursor, in particular glycosylated natural dye or natural dye precursor, and wherein the second composition comprises at least one β-glucosidase (EC 3.2.1.21). The invention also relates to a method for non-oxidative dyeing of keratin fibers, in particular human hair, using a cosmetic product according to the invention.

Description

[0001] Henkel AG & Co. KGaA

[0002] Dr. Dieckmann / PS

[0003] 2024P00252WG - 1 -

[0004] TWO-COMPONENT HAIR DYE FOR NON-OXIDATIVE HAIR COLOR IV

[0005] DESCRIPTION

[0006] Introduction

[0007] The invention relates to a cosmetic agent for the non-oxidative dyeing of keratin fibers, in particular human hair, comprising two separate compositions, wherein the first composition comprises at least one glycosylated dye or dye precursor and wherein the second composition comprises at least one β-glucosidase (EC 3.2.1.21). The invention further relates to a method for the non-oxidative dyeing of keratin fibers, in particular human hair, using a cosmetic agent according to the invention.

[0008] The desire to change one's hair color is a major need for many consumers. To satisfy this need, the cosmetics industry offers a diverse range of products. Hair dyes that achieve particularly long-lasting color with high coverage are usually oxidative dyes. These use oxidizing agents, which, however, can damage the hair structure. Certain cationic direct-acting azo dyes are also capable of achieving hair color changes with excellent colorfastness. However, these azo dyes are synthetic dyes.

[0009] A growing number of consumers desire hair dyes and coloring methods based on natural dyes, even though these products and methods are often inferior to the aforementioned products and methods in terms of colorfastness, coverage, and range. Coloring human hair with natural products is therefore enjoying increasing popularity. A growing number of consumers also value sustainable products and products without animal-derived ingredients.

[0010] The production of shelf-stable cosmetic products containing natural plant material or plant-derived natural colorants is often difficult. The plant material contains, for example, enzymes that can react with other components of the plant material or the cosmetic product, even during storage.

[0011] Henna as a plant-based dye has been known since antiquity. Traditionally, it is used as a hair dye in the form of a fine powder made from ground henna leaves. This powder is mixed with hot water to form a paste and then applied to the top layer of hair. Compared to oxidative hair dyes, this dyeing process is more cumbersome and time-consuming.

[0012] When dyeing with indigo-producing plant materials, it is frequently observed that the color changes continuously over a period of up to two weeks after application, and the final shade only becomes established after this time. At a concentration of 2% by weight of indigo-producing plant powder in water, the color on the hair changes in the first few days after dyeing from an initial turquoise-blue tone to a more reddish and darker shade, resulting in a purplish-colored appearance.

[0013] US 2004 / 0055096 A1 discloses a dye in the production of which powdered leaves of an indigo-producing plant, such as Polygonum tinctorium, Strobilanthes cusia, Indigofera tinctoria or Isatis indigotica, whose natural enzyme content has been deactivated by heat treatment, are mixed shortly before application with a β-glucosidase-containing component (e.g. fresh leaves of an indigo-producing plant that have not been heat-treated, or the pressed juice from these unheat-treated leaves, possibly in freeze-dried form, or extracts of various mushrooms, such as enokitake (Flammulina velutipes) and shiitake (Lentinus edodes), or extracts of corn leaves, apricot kernels or almonds). By separating the indigo precursor indican, which is present in the plants in the form of 3-indoxyl-β-D-glucopyranoside, from the plant's own enzyme β-glucosidase, improved storage stability of the natural dye is achieved.

[0014] In WO 2014 / 104301 A1, a similar method for improved storage stability is disclosed as in US 2004 / 0055096 A1, namely the combination of two different indigo powders, wherein the natural enzyme content in the first leaf powder component was deactivated by heat treatment, while the second leaf powder component was dried at temperatures below 50°C and thus contained active enzyme.

[0015] German patent application DE 102022126932 A1 describes an extraction method for glycosylated lawsone from the plant Lawsonia inermis. German patent application DE 102022126931 A1 describes a method for stabilizing glycosylated lawsone by inhibiting / inactivating the plant's own β-glucosidases.

[0016] In WO 2024 / 078832 A1, a two-component composition for dyeing keratinous fibers is taught, wherein a first composition contains a henna dye precursor and a second composition contains cellulase.

[0017] In WO 2024 / 188833 A1, a two-component composition for dyeing keratinous fibers is taught, wherein a first composition contains an indican and a second composition contains a cellulase.

[0018] Therefore, there remains a need to provide non-oxidative hair coloring products with improved handling and coloring properties. In particular, there remains a need to provide non-oxidative hair coloring products based on natural dyes with improved handling and coloring properties.

[0019] The object of the invention is to provide a cosmetic agent for the non-oxidative coloring of keratin fibers, in particular human hair, using dyes, especially natural dyes, which ensures easier and improved application. Furthermore, the invention aims to provide an improved coloring process for keratin fibers, in particular human hair, specifically an improved coloring process for keratin fibers, in particular human hair, based on natural dyes.

[0020] Another object of the present invention was to provide means, kits and methods for the non-oxidative coloring of keratinous fibers, in particular human hair, using dyes, especially natural dyes, with improved storage stability.

[0021] Surprisingly, it was found that not only the handling and storage stability of the product but also the hair coloring result can be improved when a two-component system consisting of "color gel" (comprising a glycosylated dye or dye precursor, in particular a glycosylated natural dye or natural dye precursor) and "enzyme gel" (comprising a

[0022] β-glucosidase) a specific thickening system is used.

[0023] The problem underlying the invention is solved by the subject matter of claim 1. A first subject matter of the invention is therefore a cosmetic agent for non-oxidative dyeing of keratinous fibers, in particular human hair, comprising two separate compositions, wherein

[0024] (A) the first composition comprises, in each case based on the total weight of the first composition,

[0025] a) at least one glycosylated dye, in particular a glycosylated natural dye, preferably in an amount of about 0.1 to about 25 wt.%, more preferably about 0.2 to about 15 wt.%, further preferably about 0.3 to about 2 wt.%, particularly preferably about 0.4 to about 1.4 wt.%, b) at least one first thickener, preferably in an amount of about 0.1 to about 12 wt.%, more preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%,

[0026] c) optionally at least one preservative, preferably in an amount of about 0.1 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%, and

[0027] d) at least one organic solvent, preferably in an amount of about 2 to about 50 wt.%, more preferably about 4 to about 50 wt.%, further preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%, wherein the organic solvent consists of C1-4-alkanols and C 2-4 -Polyols, in particular ethanol, isopropanol, n-propanol, ethylene glycol, 1,2-propanediol, glycerol and 1,3-butylene glycol, and mixtures thereof;

[0028] e) Water, preferably in an amount of more than 45 wt.%, preferably more than 50 wt.%, further preferably more than 60 wt.%, particularly preferably more than 70 wt.% and most particularly preferably up to 75 wt.%; and

[0029] (B) the second composition comprises, in each case based on the total weight of the second composition, a) at least one β-glucosidase (EC 3.2.1.21), preferably in an amount of about 0.001 to about 2.5 wt.%, more preferably about 0.01 to about 2 wt.%, further preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.5 to about 1 wt.%, based on active enzyme protein, b) at least one second thickener, preferably in an amount of about 0.1 to about 12 wt.%, more preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%,

[0030] c) optionally at least one preservative, preferably in an amount of about 0.1 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%, and

[0031] d) at least one organic solvent, preferably in an amount of about 2 to about 50 wt.%, more preferably about 4 to about 50 wt.%, further preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%, wherein the organic solvent consists of C1-4-alkanols and C 2-4 -Polyols, in particular ethanol, isopropanol, n-propanol, ethylene glycol, 1,2-propanediol, glycerol and 1,3-butylene glycol, and mixtures thereof;

[0032] e) Water, preferably in an amount of more than 40 wt.%, preferably more than 45 wt.%, further preferably more than 50 wt.%, particularly preferably more than 60 wt.% and most particularly preferably up to 65 wt.%

[0033] where the first thickener and / or the second thickener is microcrystalline cellulose.

[0034] In preferred embodiments, the first composition has a pH value of about 3 to about 8, preferably about 3.5 to about 7.5, more preferably about 4 to about 7, particularly preferably about 4 to about 6.6, each measured at 20°C, and / or the second composition has a pH value of about 2.5 to about 10, preferably about 3 to about 9.5, more preferably about 3.5 to about 9.5, particularly preferably about 4 to about 9, each measured at 20°C.

[0035] In preferred embodiments, the first thickener is the same as the second thickener.

[0036] In preferred embodiments, the first thickener and the second thickener are different.

[0037] In preferred embodiments, the first thickener is microcrystalline cellulose and the second thickener is selected from (i) a mixture of xanthan gum and scleroglucan gum, (ii) diutan gum, (iii) clay minerals, (iv) a mixture of clay minerals and xanthan gum, and (v) mixtures thereof.

[0038] In preferred embodiments, the second thickener is microcrystalline cellulose and the first thickener is selected from (i) a mixture of xanthan gum and scleroglucan gum, (ii) diutan gum, (iii) clay minerals, (iv) a mixture of clay minerals and xanthan gum, and (v) mixtures thereof.

[0039] In preferred embodiments, the first composition and / or the second composition is a water-based gel.

[0040] In preferred embodiments, the glycosylated dye is a glycosylated natural dye obtained from plant parts, powder, or extract, selected from Indigofera, in particular Indigofera tinctoria, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha “Gerardiana”, Indigofera argentea, or Indigofera longiracemosa; Isatis, in particular Isatis tinctoria (woad); Persicaria, in particular Persicaria tinctoria (dyer's knotweed); Wrightia, in particular Wrightia tinctoria; Calanthe, in particular Calanthe veratrifolia; and Baphicacanthus cusia (synonym Strobilanthes cusia); Lawsonia, in particular Lawsonia inermis; and mixtures thereof, particularly preferably from Indigofera, in particular Indigofera tinctoria. Lawsonia, especially Lawsonia inermis; and mixtures thereof.

[0041] In preferred embodiments, plant parts, powder or extract contain the glycosylated natural dye in an amount equal to or greater than 6 wt.%, preferably equal to or greater than 10 wt.%, and / or the non-glycosylated form of the natural dye in an amount equal to or less than 0.2 wt.%, preferably equal to or less than 0.1 wt.%.

[0042] In preferred embodiments, the first composition is essentially free of enzymes, preferably free of enzymes.

[0043] In preferred embodiments, the second composition is essentially free of cellulases (EC 3.2.1.4), preferably free of cellulases (EC 3.2.1.4).

[0044] In another aspect, the invention relates to a cosmetic product comprising two separate compositions, wherein

[0045] (A) the first composition comprises, in each case based on the total weight of the first composition,

[0046] a) at least one glycosylated natural dye in an amount of about 0.1 to about 25 wt.%, preferably about 0.2 to about 15 wt.%, more preferably about 0.3 to about 2 wt.%, particularly preferably about 0.4 to about 1.4 wt.%, wherein the glycosylated natural dye is selected from hennoside and / or indican,

[0047] b) at least one first thickener in an amount of about 1 to about 12 wt.%, preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%,

[0048] c) at least one preservative in an amount of about 0.1 to about 2 wt.%, preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%, wherein the preservative is selected from 1,2-octanediol, sodium benzoate and mixtures thereof, and d) 1,2-propanediol in an amount of about 2 to about 50 wt.%, preferably about 4 to about 50 wt.%, more preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%;

[0049] e) Water in an amount of more than 45 wt.%, preferably more than 50 wt.%, further preferably more than 60 wt.%, particularly preferably more than 70 wt.% and most particularly preferably up to 75 wt.%; and

[0050] (B) the second composition comprises, in each case based on the total weight of the second composition, a) at least one β-glucosidase (EC 3.2.1.21) in an amount of about 0.001 to about 2.5 wt.%, preferably about 0.01 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.5 to about 1 wt.%, based on active enzyme protein,

[0051] b) at least one second thickener in an amount of about 1 to about 12 wt.%, preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%,

[0052] c) at least one preservative in an amount of about 0.1 to about 2 wt.%, preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%, wherein the preservative is selected from 1,2-octanediol, sodium benzoate and mixtures thereof, and d) 1,2-propanediol in an amount of about 2 to about 50 wt.%, preferably about 4 to about 50 wt.%, more preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%;

[0053] e) Water in an amount of more than 40 wt.%, preferably more than 45 wt.%, further preferably more than 50 wt.%, particularly preferably more than 60 wt.% and most particularly preferably up to 65 wt.%

[0054] where the first thickener and / or the second thickener is microcrystalline cellulose.

[0055] In another aspect, the invention relates to a method for the non-oxidative dyeing of keratinous fibers, in particular human hair, comprising the following process steps in the specified order.

[0056] (a) Providing a first composition, wherein the first composition is as described herein,

[0057] (b) Providing a second composition, the second composition being as described herein,

[0058] (c) Mixing the first composition from step (a) with the second composition from step (b) to produce a ready-to-use dye, preferably in a ratio of 15:1 to 3:1, more preferably 12:1 to 5:1, more preferably 10:1 to 7:1, particularly preferably 9:1,

[0059] (d) optionally incubate the dye from step (c) for 0 to about 45 minutes, preferably about 5 minutes to about 30 minutes, preferably about 10 minutes to about 20 minutes,

[0060] (e) Applying the dye obtained in step (c) to the keratin fibers, wherein the application of the dye obtained in step (c) to the keratin fibers takes place preferably within about 1 second to about 30 minutes, preferably within about 10 seconds to about 15 minutes after completion of step (c) or (d),

[0061] (f) Allowing the dye to act on the keratin fibers, wherein the acting time is preferably about 30 seconds to about 90 minutes, more preferably about 5 minutes to about 60 minutes, more preferably about 15 minutes to about 45 minutes, and / or wherein the acting preferably takes place at a temperature of about 25°C to about 60°C, more preferably about 30°C to about 50°C, more preferably about 35°C to about 40°C, more preferably about 30°C to about 40°C, most preferably about 37°C, (g) rinsing the keratin fibers with water, and

[0062] (h) optional drying of the keratinous fibers.

[0063] These and other aspects, features, and advantages of the invention will become apparent to the person skilled in the art upon studying the following detailed description and claims. Each feature from one aspect of the invention can be incorporated into any other aspect of the invention. Furthermore, it is understood that the examples contained herein are intended to describe and illustrate the invention, but do not limit it, and in particular, the invention is not limited to these examples.

[0064] Definitions

[0065] Unless otherwise stated, all percentages are weight percent (wt%).

[0066] Numeric ranges specified in the format "from x to y" include the stated values. If multiple preferred numeric ranges are specified in this format, it is understood that all ranges resulting from the combination of the different endpoints are also included.

[0067] "At least one," as used herein, means one or more, i.e., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or more. When referring to an ingredient, the statement refers to the type of ingredient and not the absolute number of molecules. Thus, "at least one enzyme" means, for example, at least one type of enzyme, i.e., it may refer to one type of enzyme or a mixture of several different enzymes. When used with weight specifications, the statement refers to all compounds of the specified type contained in a product, i.e., the product typically does not contain any further compounds of that type beyond the specified amount of the relevant compounds.

[0068] "About", "approximately" or "about", as used herein in reference to a numerical value, refer to the corresponding numerical value ±10%, preferably ±5%.

[0069] "Essentially free from" means, unless otherwise specified, that the composition or agent contains less than 1 wt.%, preferably less than 0.5 wt.%, more preferably less than 0.1 wt.% and particularly preferably less than 0.01 wt.% of the corresponding substance, based on the total weight of the agent or composition.

[0070] "Liquid," as used herein, includes liquids and gels as well as pasty compositions. It is preferred that the liquid compositions are free-flowing and pourable at room temperature, but it is also possible that they exhibit a yield point.

[0071] A substance, e.g., a composition or an agent, is, according to the invention, solid if it exists in the solid state at 25°C and 1013 mbar. A substance, e.g., a composition or an agent, is, according to the invention, liquid if it exists in the liquid state at 25°C and 1013 mbar. "Liquid" here also includes gel-like states.

[0072] Unless explicitly stated otherwise, the term "room temperature" within the scope of the invention is understood to mean 20°C at 1013 mbar.

[0073] According to the invention, "non-oxidative" means that no oxidizing agents other than atmospheric oxygen are used in the compositions, agents, kits, and processes according to the invention. In particular, the following oxidizing agents are excluded: hydrogen peroxide, persulfates, perbromates, percarbonates, perborates, and percarbamides. Atmospheric oxygen does not constitute an oxidizing agent in the context of the invention.

[0074] According to the invention, "non-oxidative coloring" means that the coloring is achieved without the addition of oxidizing agents other than atmospheric oxygen. Specifically excluded are the oxidizing agents hydrogen peroxide, persulfates, perbromates, percarbonates, perborates, and percarbamides. Atmospheric oxygen is not considered an oxidizing agent according to the invention.

[0075] Within the scope of the invention, "glycosylated dye" refers to a dye precursor comprising a glucose residue, wherein the glucose residue can be cleaved by a β-glucosidase, thereby converting the dye precursor into its active form. The glycosylated dye can be produced synthetically or biotechnologically, or obtained from natural sources, e.g., extracted from plants, animals, fungi, algae, or bacteria.

[0076] Within the scope of the invention, "natural dye" means a dye that can be isolated from natural sources, in particular from plants and / or parts of plants; in particular, a natural dye is not synthetically produced.

[0077] Within the scope of the invention, "glycosylated natural dye" refers to a natural dye precursor comprising a glucose residue, wherein the glucose residue can be cleaved by a β-glucosidase, thereby converting the natural dye precursor into its active form. The glycosylated natural dye can be obtained from natural sources, e.g., extracted from plants, animals, fungi, algae, or bacteria. Natural dyes derived from plants are preferred according to the invention.

[0078] Detailed description

[0079] The present invention is based on the surprising finding that not only the handling and storage stability of the agent but also the hair coloring result can be improved by using a specific thickening system in a two-component system consisting of a "color gel" (comprising a glycosylated dye or dye precursor, in particular a glycosylated natural dye or natural dye precursor) and an "enzyme gel" (comprising a β-glucosidase). The present invention therefore relates, in a first aspect, to a cosmetic agent for the non-oxidative coloring of keratinous fibers, in particular human hair, comprising two separate compositions, wherein

[0080] (A) the first composition comprises, in each case based on the total weight of the first composition,

[0081] a) at least one glycosylated dye, in particular a glycosylated natural dye, preferably in an amount of about 0.1 to about 25 wt.%, more preferably about 0.2 to about 15 wt.%, further preferably about 0.3 to about 2 wt.%, particularly preferably about 0.4 to about 1.4 wt.%, b) at least one first thickener, preferably in an amount of about 0.1 to about 12 wt.%, more preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%,

[0082] c) optionally at least one preservative, preferably in an amount of about 0.1 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%, and

[0083] d) at least one organic solvent, preferably in an amount of about 2 to about 50 wt.%, more preferably about 4 to about 50 wt.%, further preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%, wherein the organic solvent consists of C1-4-alkanols and C 2-4 -Polyols, in particular ethanol, isopropanol, n-propanol, ethylene glycol, 1,2-propanediol, glycerol and 1,3-butylene glycol, and mixtures thereof;

[0084] e) Water, preferably in an amount of more than 45 wt.%, preferably more than 50 wt.%, further preferably more than 60 wt.%, particularly preferably more than 70 wt.% and most particularly preferably up to 75 wt.%; and

[0085] (B) the second composition comprises, in each case in relation to the total weight of the second composition,

[0086] a) at least one β-glucosidase (EC 3.2.1.21), preferably in an amount of about 0.001 to about 2.5 wt.%, more preferably about 0.01 to about 2 wt.%, further preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.5 to about 1 wt.%, based on active enzyme protein, b) at least one second thickener, preferably in an amount of about 0.1 to about 12 wt.%, more preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%,

[0087] c) optionally at least one preservative, preferably in an amount of about 0.1 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%, and

[0088] d) at least one organic solvent, preferably in an amount of about 4 to about 50 wt.%, more preferably about 4 to about 50 wt.%, further preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%, wherein the organic solvent consists of C1-4-alkanols and C 2-4 -polyols, in particular ethanol, isopropanol, n-propanol, ethylene glycol, 1,2-propanediol, glycerol and 1,3-butylene glycol, and mixtures thereof; e) water, preferably in an amount of more than 40 wt.%, more preferably more than 45 wt.%, further preferably more than 50 wt.%, particularly preferably more than 60 wt.% and most particularly preferably up to 65 wt.%,

[0089] where the first thickener and / or the second thickener is microcrystalline cellulose.

[0090] First composition ("color gel")

[0091] A first composition according to the invention contains as a first essential component at least one glycosylated dye or dye precursor, in particular a glycosylated natural dye or natural dye precursor.

[0092] A first composition according to the invention contains, as a second essential component, at least one first thickener.

[0093] A first composition according to the invention may optionally contain preservatives, organic solvents, water and other suitable ingredients.

[0094] Second composition ("enzyme gel")

[0095] A second composition according to the invention contains as a first essential component at least one enzyme, wherein the enzyme is a β-glucosidase (EC 3.2.1.21; CAS 9001-22-3).

[0096] A second composition according to the invention contains, as a second essential component, at least one second thickener. The second thickener can be the same as or different from the first thickener.

[0097] A second composition according to the invention may optionally contain preservatives, organic solvents, water and other suitable ingredients.

[0098] Dye or dye precursor

[0099] A first composition according to the invention contains as a first essential component at least one glycosylated dye or dye precursor.

[0100] In preferred embodiments, a first composition according to the invention can, in principle, contain all known glycosylated dyes or dye precursors.

[0101] In preferred embodiments, a first composition according to the invention contains at least one glycosylated natural dye or natural dye precursor.

[0102] In preferred embodiments, a first composition according to the invention contains at least one glycosylated natural dye or natural dye precursor obtained from plant parts, powder or extract, wherein the plant is a natural dye-producing plant.

[0103] In preferred embodiments, a first composition according to the invention comprises at least one glycosylated natural dye or natural dye precursor obtained from plant parts, powder, or extract, wherein the plant is a natural dye-producing plant consisting of Indigofera, in particular Indigofera tinctoria, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha "Gerardiana", Indigofera argentea, or Indigofera longiracemosa; Isatis, in particular Isatis tinctoria (woad); Persicaria, in particular Persicaria tinctoria (dyer's knotweed); Wrightia, in particular Wrightia tinctoria; Calanthe, in particular Calanthe veratrifolia; and Baphicacanthus cusia (synonym Strobilanthes cusia); Lawsonia, in particular Lawsonia inermis; and mixtures thereof, particularly preferably of Indigofera, especially Indigofera tinctoria; Lawsonia, especially Lawsonia inermis; and mixtures thereof, selected.

[0104] In preferred embodiments, the glycosylated dye or dye precursor described herein and usable according to the invention, in particular glycosylated natural dye or dye precursor, is present.

[0105] Natural dye precursor, not occurring as sulfonic acid esters.

[0106] In preferred embodiments, a first composition according to the invention contains at least one glycosylated natural dye orA natural dye precursor obtained from plant parts, powder, or extract, wherein the plant is a natural dye-producing plant consisting of Indigofera, in particular Indigofera tinctoria, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha "Gerardiana", Indigofera argentea, or Indigofera longiracemosa; Isatis, in particular Isatis tinctoria (woad); Persicaria, in particular Persicaria tinctoria (dyer's knotweed); Wrightia, in particular Wrightia tinctoria; Calanthe, in particular Calanthe veratrifolia; and Baphicacanthus cusia (synonym Strobilanthes cusia); Lawsonia, in particular Lawsonia inermis; and mixtures thereof, particularly preferably of Indigofera, in particular Indigofera tinctoria; Lawsonia, in particular Lawsonia inermis; and mixtures thereof, is selected, and wherein the glycosylated natural dye or natural dye precursor is not present as a sulfonic acid ester.

[0107] The plants of the aforementioned genera and species contain no or only small amounts of natural pigment. Instead, they contain glycosidically bound natural pigment (hereinafter referred to as "glycosylated natural pigment" or "natural pigment precursor"), which is converted into natural pigment by enzymatic cleavage of the glycosidic residue. Well-known examples include indican, hennoside (glycosylated lawsone), sennoside, and apigenin glucoside. The enzymatic cleavage is carried out by an enzyme with β-glucosidase activity. The plant parts of the aforementioned genera and species that are rich in natural pigments or natural pigment precursors are the leaves of these plants. To obtain the extracts containing natural pigments (or precursors) according to the invention, the plant parts, in particular the leaves, of the aforementioned genera and species are dried and ground and used as plant powder.In preferred embodiments, glycosylated natural dyes are obtained from natural dye-producing plants or from parts of these plants using an extraction agent, wherein the extraction agent preferably consists of water, C1-4 alkanols and C. 2-4 -Polyols, in particular ethanol, isopropanol, n-propanol, ethylene glycol, 1,2-propanediol, glycerol and 1,3-butylene glycol, and mixtures thereof, are selected from the group consisting of water, ethanol and water / ethanol mixtures.

[0108] Glycosylated natural dyes can be extracted using the aforementioned polar solvents. The extraction can preferably be carried out at a temperature of approximately 60°C to approximately 200°C and / or at a pressure of approximately 20 mbar to approximately 250 mbar. Since extraction at a temperature in the range of approximately 60°C to approximately 200°C can be easily performed by heating the solvent, this procedure not only isolates the glycosylated natural dyes but also simultaneously deactivates the plant's own enzymes. Deactivation of the plant's own enzymes is desirable because it has been found that the resulting color can be significantly improved if the plant's own enzymes, which as natural products are subject to a multitude of qualitative and quantitative variations in composition, are replaced by a standardized enzyme mixture in the dye.

[0109] In preferred embodiments, plant extracts used according to the invention are essentially free of enzymes. "Essentially free of enzymes" means that the extract contains less than 0.1 wt.%, preferably less than 0.05 wt.%, more preferably less than 0.01 wt.%, and particularly preferably less than 0.001 wt.% of enzyme. In particularly preferred embodiments, plant extracts used according to the invention are free of enzymes, i.e., such extracts do not contain any enzymes naturally occurring in the plant used.

[0110] The extracted natural dyes or natural dye precursors can, after completion of the extraction procedure and separation of the solid plant parts, e.g. by filtration or decantation, not be separated from the solvent, but can be used directly as a natural dye (precursor)-containing preparation in a first composition according to the invention, optionally after concentration by narrowing the solvent or after dilution.

[0111] Alternatively, after completion of the extraction procedure and separation of the solid plant parts, e.g., by filtration or decantation, the extracted natural dyes or natural dye precursors can also be separated from the solvent, e.g., by freeze-drying or by distillation off the solvent, so that the natural dye (precursor)-containing extract is obtained in powder form. To produce a first composition according to the invention, the powdered natural dye (precursor)-containing extract can be dissolved in water and mixed with other components of the first composition.

[0112] According to the invention, dried and comminuted plant parts of a natural dye-producing plant can also be used, which have been pretreated at a temperature in the range of about 60°C to about 200°C, in particular by heating without solvents, furthermore by treatment with steam or by dry heating, preferably for a period of about 1 to about 120 minutes, more preferably about 2 to about 60 minutes, and particularly preferably about 5 to about 10 minutes. The dried and comminuted plant parts containing natural dyes are preferably used in powder form.

[0113] According to the invention, it is preferred that the powder of the natural dye-producing plant, based on its weight, consists of at least 50 wt.%, preferably at least 80 wt.%, particularly preferably 80 to 100 wt.%, from the leaves of the plant.

[0114] According to the invention, preferred plant powders have a particle size of less than 500 pm, preferably about 120 to about 200 pm, particularly preferably about 150 to about 180 pm.

[0115] According to the invention, preferred plant powders have a bulk density of about 0.20 to about 0.60 g / cm³. 3 , preferably about 0.25 to about 0.40 g / cm² 3 According to the invention, preferred plant powders have a moisture content of less than 10 wt.%, preferably about 0.1 to about 6 wt.%, and particularly preferably about 0.2 to about 3 wt.%. The moisture content is determined after drying for 3 hours at 105°C.

[0116] According to the invention, preferred powders of the natural dye-producing plant contain glycosylated natural dyes in an amount of about 2 to about 15 wt.%, preferably about 4 to about 12 wt.%, particularly preferably about 6 to about 12 wt.%, based on the weight of the powder.

[0117] According to the invention, preferred extracts of the natural dye-producing plant contain glycosylated natural dyes in an amount of about 2 to about 15 wt.%, preferably about 4 to about 12 wt.%, particularly preferably about 6 to about 12 wt.%, based on the weight of the powder.

[0118] In preferred embodiments, a first composition according to the invention contains the glycosylated natural dye-containing plant powder or extract, based on the total weight of the first composition, in an amount of about 0.01 to about 6 wt.%, preferably about 0.02 to about 5 wt.%, more preferably about 0.05 to about 4.5 wt.%, and particularly preferably about 0.1 to about 4 wt.%.

[0119] In preferred embodiments, a first composition according to the invention contains the at least one natural dye, primarily in its inactive form, in particular in its glycosylated form.

[0120] In preferred embodiments, a first composition according to the invention contains at least one glycosylated natural dye obtained from plant parts, powder or extract, wherein the plant is a natural dye-producing plant and wherein the plant parts, powder or extract contain the glycosylated natural dye in an amount of equal to or more than 6% by weight, preferably equal to or more than 10% by weight.

[0121] In preferred embodiments, a first composition according to the invention contains at least one glycosylated natural dye obtained from plant parts, powder or extract, wherein the plant is a natural dye-producing plant and wherein the plant parts, powder or extract contain the non-glycosylated form of the natural dye in an amount of equal to or less than 0.2 wt.%, preferably equal to or less than 0.1 wt.%.

[0122] In preferred embodiments, a first composition according to the invention contains at least one glycosylated natural dye obtained from plant parts, powder or extract, wherein the plant is a natural dye-producing plant and wherein the plant parts, powder or extract contain the glycosylated natural dye in an amount equal to or greater than 6 wt.%, preferably equal to or greater than 10 wt.%, and the non-glycosylated form of the natural dye in an amount equal to or less than 0.2 wt.%, preferably equal to or less than 0.1 wt.%.

[0123] In preferred embodiments, a first composition according to the invention comprises at least one glycosylated natural dye obtained from plant parts, powder, or extract, selected from Indigofera, in particular Indigofera tinctoria, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha "Gerardiana", Indigofera argentea, or Indigofera longiracemosa; Isatis, in particular Isatis tinctoria (woad); Persicaria, in particular Persicaria tinctoria (dyer's knotweed); Wrightia, in particular Wrightia tinctoria; Calanthe, in particular Calanthe veratrifolia; and Baphicacanthus cusia (synonym Strobilanthes cusia); Lawsonia, in particular Lawsonia inermis; and mixtures thereof, particularly preferably from Indigofera, in particular Indigofera tinctoria; Lawsonia, in particular Lawsonia inermis; and mixtures thereof, wherein plant parts, powder or extract contain the glycosylated natural dye in an amount equal to or greater than 6 wt.-%, preferably equal to or greater than 10% by weight, contained.

[0124] In preferred embodiments, a first composition according to the invention comprises at least one glycosylated natural dye obtained from plant parts, powder, or extract, selected from Indigofera, in particular Indigofera tinctoria, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha "Gerardiana", Indigofera argentea, or Indigofera longiracemosa; Isatis, in particular Isatis tinctoria (woad); Persicaria, in particular Persicaria tinctoria (dyer's knotweed); Wrightia, in particular Wrightia tinctoria; Calanthe, in particular Calanthe veratrifolia; and Baphicacanthus cusia (synonym Strobilanthes cusia); Lawsonia, in particular Lawsonia inermis; and mixtures thereof, particularly preferably from Indigofera, in particular Indigofera tinctoria; Lawsonia, especially Lawsonia inermis;and mixtures thereof, wherein plant parts, powder or extract contain the non-glycosylated natural pigment in an amount of 0.2% by weight or less, preferably 0.1% by weight or less.

[0125] In preferred embodiments, a first composition according to the invention comprises at least one glycosylated natural dye obtained from plant parts, powder, or extract, selected from Indigofera, in particular Indigofera tinctoria, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha "Gerardiana", Indigofera argentea, or Indigofera longiracemosa; Isatis, in particular Isatis tinctoria (woad); Persicaria, in particular Persicaria tinctoria (dyer's knotweed); Wrightia, in particular Wrightia tinctoria; Calanthe, in particular Calanthe veratrifolia; and Baphicacanthus cusia (synonym Strobilanthes cusia); Lawsonia, in particular Lawsonia inermis; and mixtures thereof, particularly preferably from Indigofera, in particular Indigofera tinctoria; Lawsonia, in particular Lawsonia inermis; and mixtures thereof, wherein plant parts, powder or extract contain the glycosylated natural dye in an amount equal to or greater than 6 wt.-%, preferably equal to or greater than 10 wt.%, and the non-glycosylated form of the natural dye in an amount equal to or less than 0.2 wt.%, preferably equal to or less than 0.1 wt.%.

[0126] In preferred embodiments, a first composition according to the invention contains at least one glycosylated dye in an amount of about 0.1 to about 25 wt.%, preferably about 0.2 to about 15 wt.%, more preferably about 0.3 to about 5 wt.%, and particularly preferably about 0.4 to about 1.4 wt.%.

[0127] In preferred embodiments, a first composition according to the invention contains at least one glycosylated natural dye obtained from plant parts, powder or extract, wherein the plant is a natural dye-producing plant, in an amount of about 0.1 to about 25 wt.%, preferably about 0.2 to about 15 wt.%, more preferably about 0.3 to about 5 wt.%, and particularly preferably about 0.4 to about 1.4 wt.%.

[0128] In preferred embodiments, a first composition according to the invention comprises at least one glycosylated natural dye obtained from plant parts, powder, or extract, selected from Indigofera, in particular Indigofera tinctoria, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha "Gerardiana", Indigofera argentea, or Indigofera longiracemosa; Isatis, in particular Isatis tinctoria (woad); Persicaria, in particular Persicaria tinctoria (dyer's knotweed); Wrightia, in particular Wrightia tinctoria; Calanthe, in particular Calanthe veratrifolia; and Baphicacanthus cusia (synonym Strobilanthes cusia); Lawsonia, in particular Lawsonia inermis; and mixtures thereof, particularly preferably from Indigofera, in particular Indigofera tinctoria; Lawsonia, in particular Lawsonia inermis; and mixtures thereof, in an amount of about 0.1 to about 25 wt.%, preferably about 0.2 to about 15 wt.%, more preferably about 0.3 to about 5 wt.%.-%, particularly preferably about 0.4 to about 1.4 wt.%.

[0129] In particularly preferred embodiments, a first composition according to the invention contains at least one glycosylated dye, in particular a glycosylated natural dye, selected from the group consisting of glycosylated anthocyanins, glycosylated flavones / flavonoids (in particular luteolin, apigenin, kaempferol, flavanomarein), and glycosylated naphthoquinones and mixtures thereof, in particular selected from indican, hennoside (glycosylated lawsone), sennoside and apigenin glucoside.

[0130] In particularly preferred embodiments, a first composition according to the invention contains at least one glycosylated dye, in particular a glycosylated natural dye, selected from the group consisting of glycosylated anthocyanins, glycosylated flavones / flavonoids (in particular luteolin, apigenin, kaempferol, flavanomarein), and glycosylated naphthoquinones and mixtures thereof, in particular selected from indican, hennoside (glycosylated lawsone), sennoside and apigenin glucoside, in an amount of about 0.1 to about 25 wt.%, preferably about 0.2 to about 15 wt.%, more preferably about 0.3 to about 5 wt.%, and particularly preferably about 0.4 to about 1.4 wt.%.

[0131] In particularly preferred embodiments, a first composition according to the invention contains at least one hennoside (glycosylated lawsone).

[0132] The primary pigment in henna is lawsone (2-hydroxy-1,4-naphthoquinone), along with other coloring quinones and phenols. Lawsone exists in plants primarily in glycosidically bound form. More precisely, in henna, the so-called hennoside, a precursor of active lawsone, is present in glycosidically bound form (see left structural formula in Scheme 1). Free lawsone (see right structural formula in Scheme 1) is the coloring species, but it is only formed through hydrolysis of the hennoside and oxidation of the aglycone (hydrolawsone) once the henna powder is mixed with water (see Scheme 1).

[0133]

[0134] R= gluc R'= R"= H

[0135] R'= gluc R = R"= H

[0136] R"= gluc R = R'= H

[0137] Scheme 1: Enzymatically catalyzed reaction of hennoside (isomeric glucoside) to lawsone (aglycone). (Scheme according to DE 102022126932 A1)

[0138] The hydrolytic cleavage of hennoside occurs enzymatically—among other things, by a plant-derived β-glucosidase—as soon as the plant powder comes into contact with water. The unbound lawsone is released, which can bind to the proteins of the hair keratin. The released form of lawsone is only reactive within a limited time window, as it can interact with the side chains of amino acids (e.g., with cysteine ​​thiols). The quinoid form of lawsone reacts continuously after release if it is not applied and bound directly to the hair fiber. This phenomenon prevents the incorporation of henna and other plant powders with glycosidically bound dyes into a stable emulsion base that intensely colors the hair, regardless of storage time.

[0139] In preferred embodiments, the glycosylated lawsone is obtained from plant parts, in particular plant leaves, wherein the plant is selected from Lawsonia inermis or other plants containing lawsone derivatives. Glycosylated lawsone is defined as any hennoside that bears one or more glycosyl groups on the oxygen atoms of the bicycle or on any oxygen atom (see left-hand structure of Scheme 1).

[0140] In preferred embodiments, the glycosylated, lawson-containing comminuted plant parts or the glycosylated, lawson-containing plant extracts are pretreated such that the plant's own β-glucosidase is deactivated. The deactivation of the plant's own β-glucosidase is preferably achieved by heat treatment, e.g., by heating the plant extract to a temperature of about 60°C to about 100°C for about 3 to 60 minutes. In preferred embodiments, the glycosylated, lawson-containing plant extracts are substantially free of enzymes. "Substantially free of enzymes" means that the extract contains less than 0.1 wt%, preferably less than 0.05 wt%, more preferably less than 0.01 wt%, and particularly preferably less than 0.001 wt% of enzyme. In particularly preferred embodiments, the extract is free of enzymes, i.e., that such extracts do not contain any enzymes naturally occurring in the plant used.

[0141] Preferred methods for extracting glycosylated lawsone are described, for example, in DE 102022126932 A1 and DE 102022126931 A1.

[0142] In preferred embodiments, a first composition according to the invention contains, based on the total weight of the first composition, at least one glycosylated lawsone in an amount of about 0.1 to about 25 wt.%, preferably about 0.2 to about 15 wt.%, more preferably about 0.3 to about 2 wt.%, and particularly preferably about 0.4 to about 1.4 wt.%.

[0143] In particularly preferred embodiments, a first composition according to the invention contains at least one indican.

[0144] The indigo precursor, or indirubin precursor, indican occurs naturally in plants. Indican is a glucoside of indoxyl and occurs naturally in some plants known as "indigo plants" or "indigo-producing plants." Synonyms for indican include 3-indoxyl-β-D-glucopyranoside, indoxyl-β-D-glucoside, 3-(β-D-glucosido)indole, or plant indican. The water-soluble indican is the glycosylated form of indoxyl (also water-soluble). The glucoside residue can be cleaved by β-glucosidase, which is also present in the corresponding plants. Through oxidative dimerization, indoxyl produces the water-insoluble natural dye indigo, or, via the intermediate isatin, the natural dye indirubin. (See Scheme 2). (2) Indoxyl

[0145]

[0146] (5) Indirubin

[0147] Scheme 2: Enzymatically catalyzed reaction of indican to indigo and indirubin. (Scheme according to US 2004 / 0055096 A1)

[0148] In preferred embodiments, the at least one indican is selected from crushed parts of an indigo-producing plant and from indican-containing extracts of an indigo-producing plant and mixtures thereof, in particular from indican-containing extracts of an indigo-producing plant.

[0149] Indigo-producing plants preferred according to the invention are selected from Indigofera, in particular Indigofera tinctoria, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha, Indigofera gerardiana, Indigofera argenta, Indigofera indica or Indigofera longiracemosa; Isatis, especially Isatis tinctoria (woad) or Isatis indigotica; Polygonum or Persicaria, especially Polygonum tinctorium or Persicaria tinctoria (dyer's knotweed); Wrightia, especially Wrightia tinctoria; Calanthe, especially Calanthe veratrifolia; and Baphicacanthus, especially Baphicacanthus cusia, synonymous Strobilanthes cusia. According to the invention, particularly preferred indigo-producing plants are selected from the genus Indigofera and in particular from Indigofera tinctoria.

[0150] In preferred embodiments, the indican-containing comminuted plant parts or indican-containing plant extracts are pretreated such that the plant's own β-glucosidase is deactivated. The deactivation of the plant's own β-glucosidase is preferably achieved by heat treatment, e.g., by heating the plant extract to a temperature of about 60°C to about 100°C for about 3 to 60 minutes. In preferred embodiments, the indican-containing plant extracts are substantially free of enzymes. "Substantially free of enzymes" means that the extract contains less than 0.1 wt%, preferably less than 0.05 wt%, more preferably less than 0.01 wt%, and particularly preferably less than 0.001 wt%, of enzyme. In particularly preferred embodiments, the extract is free of enzymes, i.e., that such extracts do not contain any enzymes naturally occurring in the plant used.

[0151] A preferred method for extracting indican is described, for example, in WO 2024 / 188836 A1. In preferred embodiments, a first composition according to the invention contains, based on the total weight of the first composition, at least one indican in an amount of about 0.1 to about 25 wt.%, preferably about 0.2 to about 15 wt.%, more preferably about 0.3 to about 2 wt.%, and particularly preferably about 0.4 to about 1.4 wt.%.

[0152] In alternative, but nevertheless preferred embodiments, a first composition according to the invention contains at least one glycosylated dye obtained from animals, fungi, algae or bacteria.

[0153] In alternative, but nevertheless preferred, embodiments, a first composition according to the invention contains at least one glycosylated dye obtained by a biotechnological process in which, in particular, bacteria, yeasts, or fungi form the glycosylated dye. Corresponding processes are described, for example, in US 2023 / 0313246 A1 or JP 2022 / 038951 A.

[0154] In alternative, but nevertheless preferred embodiments, a first composition according to the invention contains at least one glycosylated dye which is synthetically produced.

[0155] Alternatively or additionally to the aforementioned glycosylated dyes or dye precursors, in particular glycosylated natural dyes or natural dye precursors, a first composition according to the invention may, in preferred embodiments, contain at least one further dye, in particular a natural dye. Preferred examples of such dyes, in particular natural dyes, include, for example, lawsone, henna, juglone, indigo, indirubin, isatin, curcumin, spinulosin, apigenin, oceines, polyphenols or ortho-diphenols, hennotaninic acid, gallic acid, alizarin, purpurin, carminic acid, kermesic acid, lactic acid, purpurogallin, protocatecholaldehyde, carotenoids, betanin, chlorophyllin, monascus, and senna.

[0156] In preferred embodiments, a first composition according to the invention contains, alternatively or additionally to the at least one glycosylated dye, in particular a glycosylated natural dye, at least one natural dye obtained from plant parts, powder or extract, preferably selected from Cassia, in particular Cassia auriculata, preferably Cassia auriculata leaf powder or extract, Cassia obovata, preferably Cassia obovata leaf powder or extract, or Cassia angustifolia, preferably Cassia angustifolia leaf powder or extract; Indigofera, in particular Indigofera tinctoria, preferably Indigofera tinctoria leaf powder or extract, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha, Indigofera gerardiana, Indigofera argenta or Indigofera longiracemosa; Isatis, especially Isatis tinctoria (woad) or Isatis indigotica;Polygonum or Persicaria, in particular Polygonum tinctorium or Persicaria tinctoria (dyer's knotweed); Wrightia, in particular Wrightia tinctoria; Calanthe, in particular Calanthe veratrifolia; Baphicacanthus cusia (synonym Strobilanthes cusia); Lawsonia, in particular Lawsonia inermis, preferably Lawsonia inermis leaf powder or extract; Juglans, in particular Juglans nigra, preferably Juglans nigra shell powder or extract (black walnut powder); Punica, in particular Punica granatum, preferably Punica granatum shell powder or extract (pomegranate fruit peel powder or extract); Uncaria, in particular Uncaria gambir, preferably Uncaria gambir leaf or twig powder or extract;

[0157] Terminalia, in particular Terminalia chebula (haritaki powder or extract), preferably Terminalia chebula fruit powder or extract; Jacat, in particular Jacat curcas, preferably Jacat curcas leaf powder or extract; Quercus, in particular Quercus infectoria (gallnut powder or extract); Bengal quince; Rheum, in particular Rheum emodi, preferably Rheum emodi root powder or extract, or Rheum palmatum, preferably Rheum palmatum root powder or extract; Adhatoda, in particular Adhatoda vasica, preferably Adhatoda vasica leaf powder or extract; Aloe, in particular Aloe barbadensis, preferably Aloe barbadensis leaf powder or extract; Brazilwood powder or extract;

[0158] Pernambuco wood powder or extract; Nicaragua wood powder or extract; Cesalpinia sappan wood powder or extract; Cordia, in particular Cordia dichotoma, preferably Cordia dichotoma leaf powder or extract; Amla fruit powder or extract; Bhringaraj leaf powder or extract; Neem leaf powder or extract; Eucalyptus, in particular Eucalyptus globulus, preferably Eucalyptus globulus leaf powder or extract; Chamomile flower powder or extract; Emblica, in particular Emblica officinalis, preferably Emblica officinalis fruit powder or extract; Hibiscus, in particular Hibiscus sabdariffa, preferably Hibiscus sabdariffa flower powder or extract; Sorghum, in particular Sorghum bicolor, preferably Sorghum bicolor powder or extract; Reseda powder or extract; Coreops / s powder or extract; Eclipta, especially Eclipta prostrata, preferably Eclipta prostrata powder or extract;Acacia, in particular Acacia concinna, preferably Acacia concinna fruit powder or extract, or Acacia catechu, preferably Acacia catechu wood powder or extract; Prunus, in particular Prunus persica, preferably Prunus persica fruit powder or extract, or Prunus domestica, preferably Prunus domestica fruit powder or extract, or Prunus serotina, preferably Prunus serotina fruit powder or extract; Coffea, in particular Coffea arabica, preferably Coffea arabica seed powder or extract; Rubia, in particular Rubia cordifolia, preferably Rubia cordifolia root powder or extract; Citrus, in particular Citrus medica, preferably Citrus medica vulgaris fruit powder or extract; Redwood powder or extract; Logwood powder or extract; Yellowwood powder or extract; Beta, in particular Beta vulgaris, preferably Beta vulgaris root powder or extract; Camellia, especially Camellia sinensis, preferably Camellia sinensis leaf powder or extract;Sapindus, in particular Sapindus mukurossi, preferably Sapindus mukurossi fruit powder or extract; Phyllanthus, in particular Phyllanthus emblica, preferably Phyllanthus emblica fruit powder or extract; Sesamum, in particular Sesamum indicum, preferably Sesamum indicum seed powder or extract; Morus, in particular Morus nigra, preferably Morus nigra fruit powder or extract, or Morus alba, preferably Morus alba bark powder or extract; Tuber, in particular Tuber melanosporum, preferably Tuber melanosporum powder or extract; Nigella, in particular Nigella sativa, preferably Nigella sativa seed powder or extract; and mixtures thereof.

[0159] In particularly preferred embodiments, a first composition according to the invention contains, alternatively or additionally to the at least one glycosylated dye, in particular a glycosylated natural dye, at least one natural dye consisting of

[0160] obtained from plant parts, powder or extract, preferably selected from Cassia angustifolia leaf powder or extract, Indigofera tinctoria leaf powder or extract, Lawsonia inermis leaf powder or extract, Punica granatum peel powder or extract (pomegranate fruit peel powder).

[0161] or extract), Terminalia chebula fruit powder or extract, Rheum palmatum root powder or extract, Emblica officinalis fruit powder or extract, Hibiscus sabdariffa flower powder or extract, Sorghum bicolor powder or extract, Reseda powder or extract, Coreopsis powder or extract, Eclipta prostrata powder or extract, Acacia concinna fruit powder or extract, Acacia catechu wood powder or extract, Prunus persica fruit powder or extract, Prunus domestica fruit powder or extract, Coffea arabica seed powder or extract, Rubia cordifolia root powder or extract, Citrus medica vulgaris fruit powder or extract, redwood powder or extract, logwood powder or extract, yellowwood powder or extract and mixtures thereof.

[0162] In preferred embodiments, a first composition according to the invention may contain only one glycosylated natural dye obtained from plant parts, powder, or extract, selected from Indigofera, in particular Indigofera tinctoria, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha "Gerardiana", Indigofera argentea, or Indigofera longiracemosa; Isatis, in particular Isatis tinctoria (woad); Persicaria, in particular Persicaria tinctoria (dyer's knotweed); Wrightia, in particular Wrightia tinctoria; Calanthe, in particular Calanthe veratrifolia; and Baphicacanthus cusia (synonym Strobilanthes cusia); Lawsonia, in particular Lawsonia inermis; and mixtures thereof, particularly preferably from Indigofera, in particular Indigofera tinctoria. Lawsonia, especially Lawsonia inermis; and mixtures thereof.

[0163] In preferred embodiments, a first composition according to the invention can contain two or more natural dyes or natural dye precursors obtained from plant parts, powders, or extracts, preferably selected from Cassia, in particular Cassia auriculata, preferably Cassia auriculata leaf powder or extract, Cassia obovata, preferably Cassia obovata leaf powder or extract, or Cassia angustifolia, preferably Cassia angustifolia leaf powder or extract; Indigofera, in particular Indigofera tinctoria, preferably Indigofera tinctoria leaf powder or extract, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha, Indigofera gerardiana, Indigofera argenta, or Indigofera longiracemosa; Isatis, in particular Isatis tinctoria (woad) or Isatis indigotica; Polygonum or Persicaria, especially Polygonum tinctorium or Persicaria tinctoria (dyer's knotweed); Wrightia, especially Wrightia tinctoria;Calanthe, in particular Calanthe veratrifolia; Baphicacanthus cusia (synonym Strobilanthes cusia); Lawsonia, in particular Lawsonia inermis, preferably Lawsonia inermis leaf powder or extract; Juglans, in particular Juglans nigra, preferably Juglans nigra shell powder or extract (black walnut powder); Punica, in particular Punica granatum, preferably Punica granatum shell powder or extract (pomegranate fruit peel powder or extract); Uncaria, in particular Uncaria gambir, preferably Uncaria gambir leaf or twig powder or extract; Terminalia, in particular Terminalia chebula (haritaki powder or extract), preferably Terminalia chebula fruit powder or extract; Jacat, in particular Jacat curcas, preferably Jacat curcas leaf powder or extract; Quercus, in particular Quercus infectoria (gallnut powder or extract); Bengal quince;Rheum, in particular Rheum emodi, preferably Rheum emodi root powder or extract, or Rheum palmatum, preferably Rheum palmatum root powder or extract; Adhatoda, in particular Adhatoda vasica, preferably Adhatoda vasica leaf powder or extract; Aloe, in particular Aloe barbadensis, preferably Aloe barbadensis leaf powder or extract; Brazilwood powder or extract; Pernambuco wood powder or extract;

[0164] Nicaragua wood powder or extract; Cesalpinia sappan wood powder or extract; Cordia, in particular Cordia dichotoma, preferably Cordia dichotoma leaf powder or extract; Amla fruit powder or extract; Bhringaraj leaf powder or extract; Neem leaf powder or extract; Eucalyptus, in particular Eucalyptus globulus, preferably Eucalyptus globulus leaf powder or extract; Chamomile flower powder or extract; Emblica, in particular Emblica officinalis, preferably Emblica officinalis fruit powder or extract; Hibiscus, in particular Hibiscus sabdariffa, preferably Hibiscus sabdariffa flower powder or extract; Sorghum, in particular Sorghum bicolor, preferably Sorghum bicolor powder or extract; Reseda powder or extract; Coreopsis powder or extract; Eclipta, in particular Eclipta prostrata, preferably Eclipta prostrata powder or extract; Acacia, especially Acacia concinna, preferably Acacia concinna fruit powder or extract, or Acacia catechu,preferably Acacia catechu wood powder or extract; Prunus, in particular Prunus persica, preferably Prunus persica fruit powder or extract, or Prunus domestica, preferably Prunus domestica fruit powder or extract, or Prunus serotina, preferably Prunus serotina fruit powder or extract; Coffea, in particular Coffea arabica, preferably Coffea arabica seed powder or extract; Rubia, in particular Rubia cordifolia, preferably Rubia cordifolia root powder or extract; Citrus, in particular Citrus medica, preferably Citrus medica vulgaris fruit powder or extract; redwood powder or extract; logwood powder or extract; yellowwood powder or extract; Beta, in particular Beta vulgaris, preferably Beta vulgaris root powder or extract; Camellia, in particular Camellia sinensis, preferably Camellia sinensis leaf powder or extract; Sapindus, in particular Sapindus mukurossi, preferably Sapindus mukurossi fruit powder or extract; Phyllanthus, in particular Phyllanthus emblica,preferably Phyllanthus emblica fruit powder or extract; Sesamum, in particular Sesamum indicum, preferably Sesamum indicum seed powder or extract; Morus, in particular Morus nigra, preferably Morus nigra fruit powder or extract, or Morus alba, preferably Morus alba bark powder or extract; Tuber, in particular Tuber melanosporum, preferably Tuber melanosporum powder or extract; Nigella, in particular Nigella sativa, preferably Nigella sativa seed powder or extract; and mixtures thereof, especially preferably of Cassia angustifolia leaf powder or extract, Indigofera tinctoria leaf powder or extract, Lawsonia inermis leaf powder or extract, Punica granatum peel powder or extract (pomegranate fruit peel powder or extract), Terminalia chebula fruit powder or extract, Rheum palmatum root powder or extract, Emblica officinalis fruit powder or extract, Hibiscus sabdariffa flower powder or extract, Sorghum bicolor powder or extract,Reseda powder or extract, Coreopsis powder or extract, Eclipta prostrata powder or extract, Acacia concinna fruit powder or extract, Acacia catechu wood powder or extract, Prunus persica fruit powder or extract, Prunus domestica fruit powder or extract, Coffea arabica seed powder or extract, Rubia cordifolia root powder or extract, Citrus medica vulgaris fruit powder or extract, redwood powder or extract, logwood powder or extract, yellowwood powder or extract, and mixtures thereof.

[0165] In preferred embodiments, a first composition according to the invention can contain two or more natural dyes or natural dye precursors,

[0166] wherein the first natural dye or natural dye precursor is a glycosylated natural dye obtained from plant parts, powder or extract, selected from Indigofera, in particular Indigofera tinctoria, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha “Gerardiana”, Indigofera argentea or Indigofera longiracemosa; Isatis, in particular Isatis tinctoria (woad); Persicaria, in particular Persicaria tinctoria (dyer's knotweed); Wrightia, in particular Wrightia tinctoria; Calanthe, in particular Calanthe veratrifolia; and Baphicacanthus cusia (synonym Strobilanthes cusia); Lawsonia, in particular Lawsonia inermis; and mixtures thereof, particularly preferably from Indigofera, in particular Indigofera tinctoria; Lawsonia, especially Lawsonia inermis; and mixtures thereof; and

[0167] wherein the second natural dye or natural dye precursor is obtained from plant parts, powder or extract selected from Cassia, in particular Cassia auriculata, preferably Cassia auriculata leaf powder or extract, Cassia obovata, preferably Cassia obovata leaf powder or extract, or Cassia angustifolia, preferably Cassia angustifolia leaf powder or extract; Indigofera, in particular Indigofera tinctoria, preferably Indigofera tinctoria leaf powder or extract, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha, Indigofera gerardiana, Indigofera argenta or Indigofera longiracemosa; Isatis, in particular Isatis tinctoria (woad) or Isatis indigotica; Polygonum or Persicaria, especially Polygonum tinctorium or Persicaria tinctoria (dyer's knotweed); Wrightia, especially Wrightia tinctoria; Calanthe, especially Calanthe veratrifolia; Baphicacanthus cusia (synonym Strobilanthes cusia);Lawsonia, in particular Lawsonia inermis, preferably Lawsonia inermis leaf powder or extract; Juglans, in particular Juglans nigra, preferably Juglans nigra shell powder or extract (black walnut powder); Punica, in particular Punica granatum, preferably Punica granatum shell powder or extract (pomegranate fruit peel powder or extract); Uncaria, in particular Uncaria gambir, preferably Uncaria gambir leaf or twig powder or extract;

[0168] Terminalia, in particular Terminalia chebula (haritaki powder or extract), preferably Terminalia chebula fruit powder or extract; Jacat, in particular Jacat curcas, preferably Jacat curcas leaf powder or extract; Quercus, in particular Quercus infectoria (gallnut powder or extract); Bengal quince; Rheum, in particular Rheum emodi, preferably Rheum emodi root powder or extract, or Rheum palmatum, preferably Rheum palmatum root powder or extract; Adhatoda, in particular Adhatoda vasica, preferably Adhatoda vasica leaf powder or extract; Aloe, in particular Aloe barbadensis, preferably Aloe barbadensis leaf powder or extract; Brazilwood powder or extract;

[0169] Pernambuco wood powder or extract; Nicaragua wood powder or extract; Cesalpinia sappan wood powder or extract; Cordia, in particular Cordia dichotoma, preferably Cordia dichotoma leaf powder or extract; Amla fruit powder or extract; Bhringaraj leaf powder or extract; Neem leaf powder or extract; Eucalyptus, in particular Eucalyptus globulus, preferably Eucalyptus globulus leaf powder or extract; Chamomile flower powder or extract; Emblica, in particular Emblica officinalis, preferably Emblica officinalis fruit powder or extract; Hibiscus, in particular Hibiscus sabdariffa, preferably Hibiscus sabdariffa flower powder or extract; Sorghum, in particular Sorghum bicolor, preferably Sorghum bicolor powder or extract; Reseda powder or extract; Coreops / s powder or extract; Eclipta, in particular Eclipta prostrata, preferably Eclipta prostrata powder or extract; Acacia, in particular Acacia concinna, preferably Acacia concinna fruit powder or extract,or Acacia catechu, preferably Acacia catechu wood powder or extract; Prunus, in particular Prunus persica, preferably Prunus persica fruit powder or extract, or Prunus domestica, preferably Prunus domestica fruit powder or extract, or Prunus serotina, preferably Prunus serotina fruit powder or extract; Coffea, in particular Coffea arabica, preferably Coffea arabica seed powder or extract; Rubia, in particular Rubia cordifolia, preferably Rubia cordifolia root powder or extract; Citrus, in particular Citrus medica, preferably Citrus medica vulgaris fruit powder or extract; Redwood powder or extract; Logwood powder or extract; Yellowwood powder or extract; Beta, in particular Beta vulgaris, preferably Beta vulgaris root powder or extract; Camellia, in particular Camellia sinensis, preferably Camellia sinensis leaf powder or extract; Sapindus, especially Sapindus mukurossi, preferably Sapindus mukurossi fruit powder or extract; Phyllanthus,in particular Phyllanthus emblica, preferably Phyllanthus emblica fruit powder or extract; Sesamum, in particular Sesamum indicum, preferably Sesamum indicum seed powder or extract; Morus, in particular Morus nigra, preferably Morus nigra fruit powder or extract, or Morus alba, preferably Morus alba bark powder or extract; Tuber, in particular Tuber melanosporum, preferably Tuber melanosporum powder or extract; Nigella, in particular Nigella sativa, preferably Nigella sativa seed powder or extract; and mixtures thereof, especially preferably of Cassia angustifolia leaf powder or extract, Indigofera tinctoria leaf powder or extract, Lawsonia inermis leaf powder or extract, Punica granatum peel powder or extract (pomegranate peel powder or extract), Terminalia chebula fruit powder or extract, Rheum palmatum root powder or extract, Emblica officinalis fruit powder or extract, Hibiscus sabdariffa flower powder or extract,Sorghum bicolor powder or extract, Reseda powder or extract, Coreopsis powder or extract, Eclipta prostrata powder or extract, Acacia concinna fruit powder or extract, Acacia catechu wood powder or extract, Prunus persica fruit powder or extract, Prunus domestica fruit powder or extract, Coffea arabica seed powder or extract, Rubia cordifolia root powder or extract, Citrus medica vulgaris fruit powder or extract, redwood powder or extract, logwood powder or extract, yellowwood powder or extract and mixtures thereof. In preferred embodiments, a first composition according to the invention can contain two or more dyes or dye precursors, in particular natural dyes or natural dye precursors, wherein the first and / or second dye or dye precursor, in particular natural dye or natural dye precursor, is derived from glycosylated anthocyanins, glycosylated flavones / flavonoids (in particular luteolin, apigenin,Kämpferol, flavanomarein), and glycosylated naphthoquinones and mixtures thereof, in particular selected from indican, hennoside (glycosylated lawsone), sennoside and apigenin glucoside.

[0170] In preferred embodiments, a first composition according to the invention may contain at least one hennoside, sennoside, apigenin glucoside and / or indican, preferably hennoside and / or indican.

[0171] In preferred embodiments, a first composition according to the invention may comprise at least one hennoside, sennoside, apigenin glucoside and / or indican, preferably hennoside and / or indican, and additionally at least one further dye or dye precursor, in particular a natural dye or natural dye precursor, selected from the group consisting of glycosylated anthocyanins, glycosylated flavones / flavonoids (in particular luteolin, apigenin, kaempferol, flavanomarein), and glycosylated naphthoquinones and mixtures thereof.

[0172] In preferred embodiments, a first composition according to the invention may comprise at least one hennoside, sennoside, apigenin glucoside and / or indican, preferably hennoside and / or indican, and additionally at least one further natural dye or natural dye precursor selected from Cassia angustifolia leaf powder or extract, Indigofera tinctoria leaf powder or extract, Lawsonia inermis leaf powder or extract, Punica granatum peel powder or extract (pomegranate fruit peel powder or extract), Terminalia chebula fruit powder or extract, Rheum palmatum root powder or extract, Emblica officinalis fruit powder or extract, Hibiscus sabdariffa flower powder or extract, Sorghum bicolor powder or extract, Reseda powder or extract, Coreopsis powder or extract, Eclipta prostrata powder or extract, Acacia concinna fruit powder or extract, Acacia catechu wood powder or extract -extract, Prunus persica fruit powder or extract,Contains Prunus domestica fruit powder or extract, Coffea arabica seed powder or extract, Rubia cordifolia root powder or extract, Citrus medica vulgaris fruit powder or extract, redwood powder or extract, logwood powder or extract, yellowwood powder or extract and mixtures thereof.

[0173] If more than one dye or dye precursor, especially natural dye or

[0174] If a natural dye precursor is contained in a first composition according to the invention, then it is contained in an amount of about 0.1 to about 25 wt.%, preferably about 0.2 to about 15 wt.%, more preferably about 0.3 to about 2 wt.%, based on the total weight of the first composition.

[0175] Alternatively, if more than one dye or dye precursor, in particular natural dye or natural dye precursor, is contained in a first composition according to the invention, these may be contained in a total amount of about 0.1 to about 25 wt.%, preferably about 0.2 to about 15 wt.%, more preferably about 0.3 to about 2 wt.%, based on the total weight of the first composition.

[0176] enzyme

[0177] A second composition according to the invention contains as a first essential component at least one enzyme, wherein the enzyme is a β-glucosidase (EC 3.2.1.21; CAS 9001-22-3).

[0178] The term "β-glucosidase," as used herein, refers to enzymes that catalyze the hydrolysis of terminal, non-reducing β-D-glucosyl residues of saccharides from the group of β-D-glucans (high-molecular-weight β-D-glucose polysaccharides, e.g., cellulose) and glycosides (glycosides are formed when an alcohol R-OH is linked to a sugar via a glycosidic bond; glycosides are compounds of the general formula ROZ, where Z is any sugar and R is selected from any sugar residue and any hydroxy compound) with the release of β-D-glucose. Such enzymes possess "β-glucosidase activity."

[0179] β-Glucosidases are found in many plants and microorganisms and enable, for example, the breakdown of cellobiose to glucose. Other names for β-glucosidase include gentiobiase, cellobiase, emulsin, elaterase, aryl-β-glucosidase, β-D-glucosidase, β-glucoside glucohydrolase, arbutinase, amygdalinase; p-nitrophenyl-β-glucosidase, primeverosidase, amygdalase, linamarase, salicilinase, and β-1,6-glucosidase.

[0180] According to the invention, the β-glucosidase activity is measured using a standard method in which β-glucosidase hydrolyzes p-nitrophenyl-β-D-glucopyranoside and the amount / increase of the hydrolysis product can be measured at 405 nm (see, for example, the commercially available test kit "β-Glucosidase Activity Assay Kit", catalog number MAK129, Sigma Aldrich).

[0181] In a second composition according to the invention, β-glucosidases (EC 3.2.1.21) are preferably of bacterial or fungal origin, more precisely from Aspergillus, Trichoderma, Penicillium, Lactobacillus acidophilus, Pseudomonas, Bacillus subtilis, or Bifidobacterium bifidum. β-glucosidases used according to the invention are particularly preferably derived from fungi selected from Aspergillus spp., in particular Aspergillus niger, Aspergillus niger SOI017, Aspergillus niger AS 3.4309, Aspergillus niger HDF 05, Aspergillus oryzae, Aspergillus strain SA 58, Aspergillus terreus EMOO 6-4, and mixtures of Aspergillus niger and Aspergillus oryzae;

[0182] Trichoderma spp., in particular Trichoderma reesei; Penicillium spp., in particular Penicillium simplicissimum H-11, Penicillium citrinum YS40-5 and Penicillium miczynskii. Further β-glucosidases used particularly preferably according to the invention are derived from the bacterium Pseudomonas, in particular Pseudomonas ZD-8. β-glucosidases from Trichoderma reesei and Aspergillus niger are particularly preferred. Chemically modified or protein-engineered mutants are included. In preferred embodiments, the β-glucosidase can be a component of an enzyme mixture which, in addition to β-glucosidase (EC 3.2.1.21), contains other enzymes, such as... B. Endo-1,4-β-glucanase (EC 3.2.1.4) and / or Exo-1,4-β-D-glucanase (EC 3.2.1.91).

[0183] In preferred embodiments, the enzyme mixture used contains, in addition to the

[0184] β-Glucosidase does not require any other enzymes.

[0185] In preferred embodiments, a second composition according to the invention contains a β-glucosidase (EC 3.2.1.21) and is substantially free of further glycosylases (glycosylases other than β-glucosidases), in particular substantially free of endo-1,4-β-glucanases (hereinafter also referred to as "cellulases"; EC 3.2.1.4). "Substantially free of" in this context means that the second composition contains less than 0.1 wt.%, preferably less than 0.05 wt.%, more preferably less than 0.01 wt.%, and most preferably less than 0.001 wt.%, further glycosylases, in particular endo-1,4-β-glucanases (EC 3.2.1.4). In particularly preferred embodiments, the second composition is free of further glycosylases, in particular free of endo-1,4-β-glucanases (EC 3.2.1.4). This means that a second composition according to the invention contains, apart from β-glucosidase (EC 3.2.1.4),21) does not include any other enzymes, in particular no other enzymes with activity on glycosidic bonds.

[0186] In preferred embodiments, a second composition according to the invention contains β-glucosidase (EC 3.2.1.21) in an amount of about 0.001 to about 2.5 wt.%, preferably about 0.01 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.5 to about 1 wt.%, based on active enzyme protein and total weight of the second composition.

[0187] In preferred embodiments, a second composition according to the invention contains β-glucosidase (EC 3.2.1.21) in an amount of about 0.001 to about 2.5 wt.%, preferably about 0.01 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.5 to about 1 wt.%, based on active enzyme protein and total weight of the second composition, wherein the β-glucosidase (EC 3.2.1.21) is derived from Aspergillus, Trichoderma, Penicillium, Lactobacillus acidophilus, Pseudomonas, Bacillus subtilis or Bifidobacterium bifidum.

[0188] The protein concentration can be determined using known methods, e.g., the BCA method (bicinchoninic acid; 2,2'-bicinolyl-4,4'-dicarboxylic acid) or the biuret method (Gornall et al., J. Biol. Chem. 177 (1948): 751-766). The determination of the active protein concentration can be carried out by titration of the active sites using a suitable irreversible inhibitor and determination of the residual activity (cf. Bender et al., J. Am. Chem. Soc. 88, 24 (1966): 5890-5913). Thickeners

[0189] A first composition according to the invention contains, as a second essential component, at least one first thickener.

[0190] A second composition according to the invention contains, as a second essential component, at least one second thickener.

[0191] In preferred embodiments, the first thickener and / or the second thickener is microcrystalline cellulose.

[0192] In preferred embodiments, the first thickener is the same as the second thickener, wherein the first thickener and the second thickener are each microcrystalline cellulose.

[0193] In preferred embodiments, the first thickener and the second thickener are different, wherein the first thickener or the second thickener is microcrystalline cellulose.

[0194] According to the invention, at least the first thickener or the second thickener is microcrystalline cellulose.

[0195] According to the invention, "microcrystalline cellulose" refers to a purified form of cellulose produced by processing α-cellulose with mineral acids (e.g., hydrochloric acid). Cellulose is a polysaccharide and consists of several hundred to tens of thousands of β-1,4-glycosidically linked β-D-glucose or cellobiose units. Although microcrystalline cellulose is chemically identical to cellulose, it exhibits different physical properties. This is because microcrystalline cellulose is partially depolymerized by the treatment with mineral acids and has a lower molar mass (approximately 220,000 g / mol), as well as a higher degree of crystallinity.

[0196] In preferred embodiments, a first and / or second composition according to the invention contains microcrystalline cellulose in an amount of about 1 to about 12 wt.%, preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%, in particular 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5 or 8 wt.%, based on the total weight of the first or second composition.

[0197] In preferred embodiments, a first and / or second composition according to the invention contains microcrystalline cellulose in an amount of about 1 to about 12 wt.%, preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%, in particular 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5 or 8 wt.%, based on the total weight of the first or second composition.

[0198] Surprisingly, it was found that the microcrystalline cellulose according to the invention is less susceptible to degradation by β-glucosidases than amorphous cellulose or commonly used modified celluloses. This makes it a suitable thickening agent for the present invention. In preferred embodiments, the first thickener and the second thickener are different, with the first thickener or the second thickener being microcrystalline cellulose.

[0199] According to the invention, at least the first thickener or the second thickener is microcrystalline cellulose.

[0200] In preferred embodiments, the first thickener is microcrystalline cellulose as described herein, and the second thickener is selected from (i) a mixture of xanthan gum and scleroglucan gum, (ii) diutan gum, (iii) clay minerals, (iv) a mixture of clay minerals and xanthan gum, and (v) mixtures thereof.

[0201] In preferred embodiments, the second thickener is microcrystalline cellulose as described herein, and the first thickener is selected from (i) a mixture of xanthan gum and scleroglucan gum, (ii) diutan gum, (iii) clay minerals, (iv) a mixture of clay minerals and xanthan gum, and (v) mixtures thereof.

[0202] Xanthan gum (CAS 11138-66-2) is a naturally occurring polysaccharide produced by bacteria of the genus Xanthomonas (e.g., Xanthomonas campestris). Xanthan gum consists of β-(1,4)-linked D-glucose units, with a β-(1,4)-linked nucleotide attached to every second glucose unit.

[0203] The α-(1,3)-glycosidic bond forms a β-D-mannopyranosyl-(1,4)-β-D-glucuronopyranosyl-(1,2)-6-O-acetyl-αD-mannopyranosyl side chain, with some of the terminal mannose units of the side chain forming a ketal with pyruvic acid via hydroxyl groups at positions 4 and 6. Commercial xanthan gum products include, for example, Keltrol® CG-SFT (formerly CP Kelco) and Xanthan Gum FNCS-PC™ (formerly Jungbunzlauer).

[0204] Scleroglucan gum (synonym: sclerotium gum; CAS 39464-87-4) is a naturally occurring polysaccharide produced by fungi of the genus Sclerotium, particularly Sclerotium rolfsii. It is a D-glucopyranose polymer consisting of β-1,3-glucose units, the main chain of which comprises β-1,3-glucose units with a β-1,6-side chain. Useful scleroglucan gums are described in, for example, WO 2016 / 091817, FR 2633940, and US 3301848. Commercial scleroglucan gum products include, for example, Actigum® CS (formerly Sanofi Bio Industries) and Amigel® (formerly Alban Müller International).

[0205] In preferred embodiments, a first or second composition according to the invention contains the thickening mixture of xanthan gum and scleroglucan gum in an amount of 3% by weight or less. In preferred embodiments, a first or second composition according to the invention contains the thickening mixture of xanthan gum and scleroglucan gum in an amount of about 0.1 to about 3% by weight, preferably about 0.5 to about 2.7% by weight, more preferably about 0.8 to about 2.4% by weight, and particularly preferably about 1 to about 1.8% by weight, based on the total weight of the first or second composition.

[0206] In preferred embodiments, the ratio of xanthan gum to scleroglucan gum is 0.6:1 to 1.5:1, preferably 0.6:1 and increasingly preferably 0.65:1, 0.7:1, 0.75:1, 0.8:1, 0.85:1, 0.9:1, 0.95:1, 1:1, 1.05:1, 1.1:1, 1.15:1, 1.2:1, 1.25:1, 1.3:1, 1.35:1, 1.4:1, 1.45:1, 1.5:1. In particularly preferred embodiments, the ratio of xanthan gum to scleroglucan gum is 1:1 to 1.5:1, most preferably 1.2:1, 1.25:1, 1.3:1 or 1.35:1.

[0207] In preferred embodiments, a first or second composition according to the invention contains the thickener mixture of xanthan gum and scleroglucan gum in an amount of equal to or less than 3% by weight, and the ratio of xanthan gum to scleroglucan gum is 0.6:1 to 1.5:1, preferably 1:1 to 1.5:1, in particular 0.6:1, and increasingly preferably 0.65:1, 0.7:1, 0.75:1, 0.8:1, 0.85:1, 0.9:1, 0.95:1, 1:1, 1.05:1, 1.1:1, 1.15:1, 1.2:1, 1.25:1, 1.3:1, 1.35:1, 1.4:1, 1.45:1, 1.5:1, most preferably 1.2:1. 1.25:1, 1.3:1 or 1.35:1.

[0208] In preferred embodiments, a first or second composition according to the invention contains the thickener mixture of xanthan gum and scleroglucan gum in an amount of about 0.1 to about 3 wt.%, preferably about 0.5 to about 2.7 wt.%, more preferably about 0.8 to about 2.4 wt.%, and particularly preferably about 1 to about 1.8 wt.%, based on the total weight of the first or second composition, respectively, and the ratio of xanthan gum to scleroglucan gum is 0.6:1 to 1.5:1, preferably 1:1 to 1.5:1, in particular 0.6:1, and increasingly preferably 0.65:1, 0.7:1, 0.75:1, 0.8:1, 0.85:1, 0.9:1, 0.95:1, 1:1, 1.05:1, 1.1:1, 1.15:1, 1.2:1. 1.25:1, 1.3:1, 1.35:1, 1.4:1, 1.45:1, 1.5:1, especially preferred 1.2:1, 1.25:1, 1.3:1 or 1.35:1.

[0209] In preferred embodiments, a first or second composition according to the invention contains xanthan gum in an amount of about 0.1 to about 1.7 wt.%, preferably about 0.25 to about 1.5 wt.%, more preferably about 0.4 to about 1.2 wt.%, particularly preferably about 0.5 to about 1 wt.%, and scleroglucan gum in an amount of about 0.1 to about 1.3 wt.%, preferably about 0.25 to about 1.2 wt.%, more preferably about 0.4 to about 1 wt.%, particularly preferably about 0.5 to about 0.8 wt.%, in each case based on the total weight of the first or second composition.

[0210] According to the invention, diutan gum (CAS 125005-87-0 and 595585-15-2) is a polysaccharide produced by the bacterium Sphingomonas, in particular Sphingomonas elodea. It is commercially available, for example, in the products GEOVIS®, KELCO-CARE®, KELCO-CRETE® and KELCO-VIS® (formerly CP Kelco).

[0211] In preferred embodiments, a first or second composition according to the invention contains diutan gum in an amount of 3% by weight or less, preferably 2.5% by weight or less, more preferably 1.5% by weight or less, and particularly preferably 1% by weight or less. In preferred embodiments, a first or second composition according to the invention contains diutan gum in an amount of about 0.1 to about 3% by weight, more preferably about 0.25 to about 2.5% by weight, more preferably about 0.5 to about 1.5% by weight, and particularly preferably about 0.5 to about 1% by weight, based on the total weight of the first or second composition, respectively. B. 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95 or 1 wt.%.According to the invention, "clay minerals" are understood to be layered silicates (phyllosilicates) that have a layered crystal structure composed of silicon and oxygen, as well as hydrogen and usually magnesium, sodium, or aluminum. Such layered silicates have the general formula R. 1 aR 2 b(R 3 2cO5c)(OH)d

[0212] up, in the

[0213] R 1 from alkali metal ions, alkaline earth metal ions, H3O + , NH4 and mixtures thereof, preferably alkali and / or alkaline earth metal ions, particularly preferably K + , N / a + , Ca 2+ and mixtures thereof, especially preferred by Na + ,

[0214] a is 0 or any number between 0 and 3, e.g. 0, 0.1, 0.20, 25, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3, preferably any number between 0 and 2; particularly preferably any number between 0 and 1, most particularly preferably 0 or 0.3,

[0215] R 2 selected from alkali, alkaline earth or transition metal ions and mixtures thereof, preferably Mg 2+ , Fe 2+ , Fe 3+ , Ni 2+ , Al 3+ , Li + , Cr, Cu, Zn, V, Mn 2+ , Mn 3+ , Ti and mixtures thereof, especially preferably Mg 2+ , Fe 2+ , Fe 3+ , Ni 2+ , Al 3+ , Li + Cr, Cu, Zn and mixtures thereof, especially Mg 2+ , Li + and mixtures thereof,

[0216] b is any number between 1 and 10, preferably any number between 1 and 3, e.g. 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3; particularly preferably 2 or 3, most particularly preferably 3.

[0217] R 3 from AI, Si, Fe 3+ , Be, Zn, B and mixtures thereof, preferably Si and / or Al, particularly preferably Si,

[0218] c is any number between 1 and 4, preferably 1 or 2; particularly preferably 2,

[0219] R 4 selected from OH, F, O, S and mixtures thereof, preferably OH or F, particularly preferably OH,

[0220] d is any number between 1 and 10, preferably any number between 1 and 4, particularly preferably 1 or 2, most particularly preferably 2,

[0221] where (R 32COSC) is preferably selected from ((Al. Si^Os), ((AI, Si)4Oi 0), (Si20s), (Si40 ) and (AlSisOw), is particularly preferably ((AI, Si)40 ) or (Si40 ), particularly preferably (Si40 ).

[0222] A common and well-known classification or grouping for minerals can be found, for example, in "Systematics of Minerals according to Strunz", 9th edition; see, for example,

[0223] https: / / www.mineralienatlas.de / lexikon / index.php / Mineralklasse-9E.

[0224] Preferred clay minerals from the group of phyllosilicates include, in particular, phyllosilicates with mica sheets (9th EC) or phyllosilicates with kaolinite sheets (9th ED). Examples of preferred clay minerals from the group of phyllosilicates with mica sheets (9th EC) or phyllosilicates with kaolinite sheets (9th ED) are:

[0225] • 9. EC.05 Talk group, especially Minnesotaite (Fe 2+3(Si4O )(OH)2), Talk (Mg3(Si4O )(OH)2), Willemseit ((Ni, Mg)3(Si40io)(OH)2); • 9. EC.10 Pyrophyllit-Gruppe, insbesondere Ferripyrophyllit (Fe 3+ (Si2Os)(OH), Pyrophyllit (AI2(Si40io)(OH)2);

[0226] • 9. EC.15 Muskovit-Gruppe, insbesondere Glaukonit ((K, Na)(Fe 3+ , AI, Mg)2((Si, AI)40io)(OH)2), Muskovit (KAl2(AISi3Oio)(OH, F)2), Nanpingit (CsAI2((Si, AI)40io)(OH, F)2), Paragonit (NaAl2(AISi3Oio)(OH)2), Roscoelith (K(V, AI, Mg)2(AISi3Oio)(OH)2), Seladonit (K(Mg, Fe 2+ )(Fe 3+ , AI)(Si40io)(OH)2), Tainiolith (KLiMg2(Si40io)F2), Tobelith ((NH4, K)Al2(AISi3Oio)(OH)2), Zweiaxiger Glimmer (KAI2((Si, AI)40io)(OH)2), Manganiseladonit (KMgMn 3+ (Si4O )(OH)2);

[0227] • 9. EC.20 Phlogopit-Gruppe, insbesondere Annit (KFe 2+ 3(AISi3O )(OH, F)2), Aspidolith (NaMg3(AISi3Oio)(OH)2), Biotit (K(Mg, Fe 2+ )3(AISi3Oio)(OH, F)2), Ephesit (NaLiAl2(Al2Si2Oio)(OH)2), Fluorannit (KFe 2+3(AISi3Oio)(OH, F)2), Hendricksite (K(Zn, Mn)3(AISi3Oio)(OH)2), Masutomilith (K(Li, AI, Mn 2+ )3((Si, AI)40io)(OH, F)2), Phlogopite (KMg3(AISi3Oio)(OH, F)2), Polylithionite (KLi2AI(Si40io)(OH, F)2), Shiroshinite (KNaMg2(Si40io)F2), Shirozulith (K(Mn 2+ , Mg)3(AISi3Oio)(OH)2), Siderophyllite (KFe 2+ 2AI(Al2Si2O )(OH, F)2), Tetraferrites (KFe 2+ 3(Si3Fe 3+ O )(OH)2), Tetraferriphlogopite (KMg3((AI, Fe 3+ )Si3Oio)(OH, F)2), Trilithionite (KLi 1.5 Al 1.5 (AlSi3O 10 )F2), Wonesite (Na, K)(Mg, Fe, AI)6((Si, AI)802o)(OH, F)4), Zinnwaldite (KLiFe 2+ AI(AISi3Oio)(OH, F)2), Fluorophlogopite (KMg3(AISi3Oio)F2), Sokolovaite (CsLi2AI(Si40io)F2), Suhailite ((NH4)(Fe 2+ )3(AISi3Oio)(OH)2), Yangzhumingite (KMg 2.5 (Si4O 10 )F2), Oxyphlogopite (K(Mg, Ti, Fe)3((Si, AI)4O )(O, F)2), Fluorotetraferriphlogopite (KMg3(Fe 3+ Si3O 10 )F2), Garmite (CsLiMg2(Si4O 10)F2), Gorbunovite (CsLi2(Ti, Fe)(Si40io)(F, OH,0)2), Craterite (CsLi2Fe 3+ (Si40io)F2);

[0228]

[0229] • 9. EC.25 Illit-Gruppe, insbesondere Brammallit (Na, H30)(AI, Mg, Fe)2((Si, AI)40io)(OH)2 H2O), lllit ((K, H30)AI2((AISi3Oio)(OH)2H2O);

[0230] • 9. EC.30 Margarit-Gruppe, insbesondere Margarite (CaAl2((Al2Si2O )(OH)2), Fluorluansheiit (KLiAl 1.5 ☐ 0.5 (Si 3.5 Al 0.5 Oh 10 )F2);

[0231] • 9. EC.35 Clintonite-Group, insbesondere Anandite ((Ba, K)(Fe 2+ , Mg)3((Si, AI, Fe)40io)(S, OH)2), Bittyite (CaLiAl2(AIBeSi2O )(OH)2), Clintonite (Ca(Mg, AI)3(AI3SiOio)(OH)2), Ferrokinoshitalith (Ba(Fe 2+ , Mg)3(Al2Si2Oio)(OH, F)2), Kinoshitalith ((Ba, K)(Mg, Mn, AI)3(Si2AI2Oio)(OH)2), Oxykinoshitalith ((Ba, K)(Mg, Fe 2+ , Ti 4+ )3((Si, AI)40io)02), Fluoroquinoshitalith (BaMg3(AI2Si2Oio)F2);

[0232] • 9. EC.40 Montmorillonit-Gruppe, insbesondere Beidellit ((Na, Cao5)o3Al2((Si, AI)40io)(OH)2'4H20), Hectorit (Nao3(Mg, Li)3(Si40 )(F, OH)2), Montmorillonit ((Na, Ca)o3(AI, Mg)2(Si40io)(OH)2 nH20), Nontronit (Nao3Fe 3+ 2((Si, AI)40io)(OH)2'4H20), Saponit ((Ca, Na)o3(Mg, Fe 2+ )3((Si, AI)40io)(OH)2'4H20), Swinefordit ((Ca, Na)o3(AI, Li, Mg)2((Si, AI)40io)(OH, F)2'2H20), Volkonskoit

[0233] (Cao3(Cr 3+ , Mg, Fe 3+ )2((Si, AI)40io)(OH)2'4H20), Yakhontovit ((Ca, Na, K)o3(Cu, Fe 2+ , Mg)2(Si40io)(OH)2'3H20);

[0234] • 9. EC.45 Saponit-Gruppe, insbesondere Ferrosaponit

[0235] (Cao3(Fe 2+ , Mg, Fe 3+ )3((Si, AI)Si3Oio)(OH)2'4H20), Sauconit (Nao3Zn3((Si, AI)40io)(OH)2-4H20), Stevensit ((Ca2)o3Mg3(Si40io)(OH)2), Zinksilit (Zn3(Si40io)(OH)2'4H20), Kerolit ((Mg, Ni)3(Si40io)(OH)2 H20;

[0236] • 9. EC.50 Vermiculite-Group, in particular Vermiculite (Mgo 7(Mg, Fe, AI)6((Si, AI)802o)(OH)4'8H20); • 9. EC.55 Chlorite Group, particularly Bailechlor ((Zn, Fe 2+ , AI, Mg)6((Si, AI)40io)(OH)8), Chamosite ((Fe 2+ , Mg, Fe 3+ )5AI(AISi3Oio)(OH,0)8), Cookeite (LiAl4(AISi3Oio)(OH)8), Delessite ((Mg, Fe, AI)((Si, AI)40io)(0, OH)8), Donbassit (Al23(AISi3O )(OH)8), Franklinfurnaceite (Ca2(Fe 3+ , AI)Mn 3+ Mn 2+ 3(Zn2Si20io)(OH)8), Glagolevite (NaMg6(AISi3Oio)(OH,0)8H20), Gonyerite (Mn 2+ 5Fe 3+ (Si3Fe 3+ Oio)(OH)8), Clinochlor ((Mg, Fe 2+ )5AI(AISi3Oio)(OH)8), Nimit ((Ni, Mg, Fe 2+ )5AI(AISi3Oio)(OH)8), Odinite ((Fe 3+ , Mg, Al, Fe 2+ , Ti, Mn) 2.4 (Flaw 1.8 Al 0.2 O5)(OH)4), Orthochamocytes ((Fe 2+ , Mg, Fe 3+ )5AI(AISi3Oio)(OH,0)8), Pennantite (Mn 2+5AI(AISi3Oio)(OH)8), Sudoite (Mg2AI3(AISi3Oio)(OH)8), Chlorite-Group ((Fe, Mg, AI)6((Si, AI)40io)(OH)8);

[0237] • 9. EC.60 Corrensite Group, in particular Aliettit (Cao2Mg6((Si, AI)802o)(OH)4'4H20), Brinrobertsite ((Na, K, Ca)o3(AI, Fe, Mg)4((Si, AI)802o)(OH)4'3. K)(Mg, Fe, AI)9((Si, AI)802o)(OH)io nH20), Dozyite (Mg7(AI, Fe 3+ , Cr)2(AI2Si4Oi5)(OH)i2), Karpinskite ((Mg, Ni)2(Si2O5)(OH)2), Kulkeite (Na 0.35 Mg8Al(AlSi7O 20 )(OH) 10 ), Lunijianlate (Li 0.7 Al 6.2 (AlSi7O 20 )(OH,O) 10 ), Rectorite ((Na, Ca)Al4((Si, AI)802o)(OH)4'2H20), Saliotite (Na 0.5 The 0.5 Al3(AlSi3O 10 )(OH)8), Hydrobiotite (K(Mg, Fe 2+ )s((Si, AI)802o)(OH)4 ■ n H2O);

[0238] • 9. ED.05 Kaolinite group, in particular Dickite (Al2(Si2Os)(OH)4), Kaolinite (Al2(Si2Os)(OH)4), Nacrete (Al2(Si2O5)(OH)4);

[0239] • 9. ED.10 Halloysite-Group, in particular Halloysite (Al2(Si2Os)(OH)4), Hisingerite (Fe 3+ 2(Si2O5)(OH)4'2H2O);

[0240] • 9. ED.15 Serpentine Group, especially Amesite (Mg2AI(SiAIOs)(OH)4), Antigorite ((Mg, Fe 2+ )3(Si2O5)(OH)4), Berthierin ((Fe 2+ , Fe 3+ , AI)3((Si, AI)2O5)(OH)4), Brindleyite ((Ni, Mg, Fe 2+ )2AI(SiAIO5)(OH)4), Chrysotile (Mg3(Si2O5)(OH)4), Cronstedtit (Fe 2+ 2Fe 3+ (SiFe 3+ O5)(OH)4), Fraipontite ((Zn, AI)3((Si, AI)2O5)(OH)4), Greenalith ((Fe 2+ , Fe 3+ )2-3(Si2O5)(OH)4), Caryopilite (Mn 2+ 3(Si2O5)(OH)4), Kellyite ((Mn 2+ , Mg, AI)3((Si, AI)2O5)(OH)4), Clinochrysotile (Mg3(Si2O5)(OH)4), Lizardite (Mg3(Si2Os)(OH)4), Manandonite (Li2Al4((Si2AlBO 10 )(OH)8), Nepouit ((Ni, Mg)3(Si2Os)(OH)4), Orthochrysotile (Mg3(Si2O5)(OH)4), Parachrysotile (Mg3(Si2O5)(OH)4), Pecoraite (Ni3(Si2O5)(OH)4).

[0241] Besonders bevorzugte Tonminerale sind die Tonminerale der Montmorillonitgruppe (9. EC.40) und der Saponit-Gruppe (9. EC.45), insbesondere umfassend Beidellit

[0242] ((Na, Cao 5)o3Al2((Si, AI)40io)(OH)2'4H20), Hectorit (Nao3(Mg, Li)3(Si40 )(F, OH)2), Montmorillonit ((Na, Ca)o3(AI, Mg)2(Si40io)(OH)2 nH20), Nontronit (Nao3Fe 3+ 2((Si, AI)40io)(OH)2'4H20), Saponit ((Ca, Na)o3(Mg, Fe 2+ )3((Si, AI)40io)(OH)2'4H20), Swinefordit

[0243] ((Ca, Na)o3(AI, Li, Mg)2((Si, AI)40io)(OH, F)2'2H20), Volkonskoit

[0244] (Cao3(Cr 3+ , Mg, Fe 3+ )2((Si, AI)40io)(OH)2'4H20), Yakhontovit ((Ca, Na, K)o3(Cu, Fe 2+ , Mg)2(Si40io)(OH)2'3H20), Ferrosaponit

[0245] (Cao3(Fe 2+ , Mg, Fe 3+)3((Si,Al)Si3Oio)(OH)2'4H2O), Sauconite (NaO3Zn3((Si,Al)4Oio)(OH)2-4H2O), Stevensite ((Ca2)O3Mg3(Si4Oio)(OH)2), Zincsilite (Zn3(Si4Oio)(OH)2'4H2O), Kerolite ((Mg,Ni)3(Si4Oio)(OH)2H2O. In preferred embodiments, a first or second composition according to the invention contains at least one thickener selected from the group of clay minerals having the following general formula

[0246] R 1 aR 2 b(R 3 2cO5c)(OH)d

[0247] up, in the

[0248] R 1 from alkali metal ions, alkaline earth metal ions, H3O + , NH4 and mixtures thereof, preferably alkali and / or alkaline earth metal ions, particularly preferably K + , N / a + , Ca 2+ and mixtures thereof, especially preferred by Na + ,

[0249] a is 0 or any number between 0 and 3, e.g. 0, 0.1, 0.20, 25, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3, preferably any number between 0 and 2; particularly preferably any number between 0 and 1, most particularly preferably 0 or 0.3,

[0250] R 2 selected from alkali, alkaline earth or transition metal ions and mixtures thereof, preferably Mg 2+ , Fe 2+ , Fe 3+ , Ni 2+ , Al 3+ , Li + , Cr, Cu, Zn, V, Mn 2+ , Mn 3+ , Ti and mixtures thereof, especially preferably Mg 2+ , Fe 2+ , Fe 3+ , Ni 2+ , Al 3+ , Li + Cr, Cu, Zn and mixtures thereof, especially Mg 2+ , Li + and mixtures thereof,

[0251] b is any number between 1 and 10, preferably any number between 1 and 3, e.g. 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3; particularly preferably 2 or 3, most particularly preferably 3.

[0252] R 3 from AI, Si, Fe 3+ , Be, Zn, B and mixtures thereof, preferably Si and / or Al, particularly preferably Si,

[0253] c is any number between 1 and 4, preferably 1 or 2; particularly preferably 2,

[0254] R 4 selected from OH, F, O, S and mixtures thereof, preferably OH or F, particularly preferably OH,

[0255] d is any number between 1 and 10, preferably any number between 1 and 4, particularly preferably 1 or 2, most particularly preferably 2,

[0256] where (R 32COSC) is preferably selected from ((Al. Si^Os), ((Al, Si)4Oi 0), (Si20s), (Si40 ) and (AlSisOw), is particularly preferably ((Al, Si)40 ) or (Si40 ), particularly preferably (Si40 ), preferably in an amount of equal to or less than 3 wt.%, preferably equal to or less than 2.5 wt.%.

[0257] In preferred embodiments, a first or second composition according to the invention comprises at least one thickener selected from the group of clay minerals having the following general formula

[0258] R 1 aR 2 b(R 3 2cO5c)(OH)d

[0259] up, in the

[0260] R 1 from alkali metal ions, alkaline earth metal ions, H3O + , NH4 and mixtures thereof, preferably alkali and / or alkaline earth metal ions, particularly preferably K + , N / a + , Ca 2+ and mixtures thereof, especially preferred by Na +, a 0 or any number between 0 and 3, e.g. 0, 0.1, 0.20, 25, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3, preferably any number between 0 and 2; particularly preferably any number between 0 and 1, most particularly preferably 0 or 0.3,

[0261] R 2 selected from alkali, alkaline earth or transition metal ions and mixtures thereof, preferably Mg 2+ , Fe 2+ , Fe 3+ , Ni 2+ , Al 3+ , Li + , Cr, Cu, Zn, V, Mn 2+ , Mn 3+ , Ti and mixtures thereof, especially preferably Mg 2+ , Fe 2+ , Fe 3+ , Ni 2+ , Al 3+ , Li + Cr, Cu, Zn and mixtures thereof, especially Mg 2+ , Li + and mixtures thereof,

[0262] b is any number between 1 and 10, preferably any number between 1 and 3, e.g. 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3; particularly preferably 2 or 3, most particularly preferably 3.

[0263] R 3 from AI, Si, Fe 3+ , Be, Zn, B and mixtures thereof, preferably Si and / or Al, particularly preferably Si,

[0264] c is any number between 1 and 4, preferably 1 or 2; particularly preferably 2,

[0265] R 4 selected from OH, F, O, S and mixtures thereof, preferably OH or F, particularly preferably OH,

[0266] d is any number between 1 and 10, preferably any number between 1 and 4, particularly preferably 1 or 2, most particularly preferably 2,

[0267] where (R 32COSC) is preferably selected from ((Al.Si^Os), ((Al, Si)40), (Si20s), (Si40) and (AlSisOw), particularly preferably ((Al, Si)40) or (Si40), particularly preferably (Si40), preferably in an amount of about 0.1 to about 3 wt.%, preferably about 0.5 to about 2.7 wt.%, further preferably about 0.8 to about 2.4 wt.%, particularly preferably about 1 to about 1.8 wt.%, in particular e.g. 1, 1.05, 1.1, 1.15, 1.2, 1.25, 1.3, 1.35, 1.4, 1.45, 1.5, 1.55, 1.6, 1.65, 1.7, 1.75, 1.8, 1.85, 1.9, 1.95, 2, 2.05, 2.1, 2.15, 2.2, 2.25, 2.3, 2.35, 2.4, 2.45 or 2.5 wt.%, based on the total weight of the first or second composition.

[0268] In preferred embodiments, a first or second composition according to the invention comprises at least one thickener selected from the group consisting of 9.EC.05 talc group, 9.EC.10 pyrophyllite group, 9.EC.15 muscovite group, 9.EC.20 phlogopite group, 9.EC.25 illite group, 9.EC.30 margarite group, 9.EC.35 clinlithite group, 9.EC.40 montmorillonite group, 9.EC.45 saponite group, 9.EC.50 vermiculite group, 9.EC.55 chlorite group, 9.EC.60 corrensite group, 9.ED.05 kaolinite group, 9.ED.10 halloysite group, 9.ED.15 serpentine group and mixtures thereof, preferably in an amount of 3% by weight or less, more preferably 2.5% by weight or less. wt.%

[0269] In preferred embodiments, a first or second composition according to the invention comprises at least one thickener selected from the group consisting of 9. EC.05 talc group, 9. EC.10 pyrophyllite group, 9. EC.15 muscovite group, 9. EC.20 phlogopite group, 9. EC.25 illite group, 9. EC.30 margarite group, 9. EC.35 clinlithite group, 9. EC.40 montmorillonite group, 9. EC.45 saponite group, 9. EC.50 vermiculite group, 9. EC.55 chlorite group, 9. EC.60 corrensite group, 9. ED.05 kaolinite group, 9. ED.10 halloysite group, 9. ED.15 serpentine group and mixtures thereof, preferably in an amount of about 0.1 to about 3 wt.%, more preferably about 0.5 to about 2.7 wt.%, more preferably about 0.8 to about 2.4 wt.%, particularly preferably about 1 to about 1.8 wt.%, in particular e.g. 1, 1.05, 1.1, 1.15, 1.2, 1.25, 1.3, 1.35, 1.4, 1.45, 1.5, 1.55, 1.6, 1.65, 1.7, 1.75, 1.8, 1.85, 1.9, 1.95, 2, 2.05, 2.1, 2.15, 2.2, 2.25, 2.3, 2.35, 2.4, 2.45 or 2.5 wt.%-%, based on the total weight of the first or second composition.

[0270] In particularly preferred embodiments, a first or second composition according to the invention contains at least one thickener selected from clay minerals of the montmorillonite group (9. EC.40) and the saponite group (9. EC.45) and mixtures thereof, preferably in an amount of 3 wt.% or less, preferably 2.5 wt.% or less.

[0271] In particularly preferred embodiments, a first or second composition according to the invention contains at least one thickener selected from clay minerals of the montmorillonite group (9. EC.40) and the saponite group (9. EC.45) and mixtures thereof, preferably in an amount of about 0.1 to about 3 wt.%, more preferably about 0.5 to about 2.7 wt.%, further preferably about 0.8 to about 2.4 wt.%, more preferably about 1 to about 1.8 wt.%, in particular, for example, in the following quantities: B. 1, 1.05, 1.1, 1.15, 1.2, 1.25, 1.3, 1.35, 1.4, 1.45, 1.5, 1.55, 1.6, 1.65, 1.7, 1.75, 1.8, 1.85, 1.9, 1.95, 2, 2.05, 2.1, 2.15, 2.2, 2.25, 2.3, 2.35, 2.4, 2.45 or 2.5 wt.%, based on the total weight of the first or second composition.

[0272] In particularly preferred embodiments, a first or second composition according to the invention comprises at least one thickener consisting of the component from Beidellit.

[0273] ((Na, Cao 5)o3Al2((Si, AI)40io)(GH)2'4H20), hectorite (Nao3(Mg, Li)3(Si40 )(F, OH)2), montmorillonite ((Na, Ca)o3(AI, Mg)2(Si40io)(OH)2 nH20), nontronite (Nao 3Fe 3+ 2((Si, AI)40io)(OH)2'4H20), saponite ((Ca, Na)o3(Mg, Fe 2+ )3((Si, AI)40io)(OH)2'4H20), swinefordite ((Ca, Na)o3(AI, Li, Mg)2((Si, AI)40io)(OH, F)2-2H20), volkonskoite

[0274] (Cao 3(Cr 3+ , Mg, Fe 3+ )2((Si, AI)40io)(OH)2'4H20), Yakhontovit ((Ca, Na, K)o3(Cu, Fe 2+ , Mg)2(Si40io)(GH)2'3H20), ferrosaponite

[0275] (Cao 3(Fe 2+ , Mg, Fe 3+ )3((Si,Al)Si3Oio)(OH)2'4H20), Sauconite (Nao3Zn3((Si,Al)40io)(OH)2-4H20), Stevensite ((Ca2)o3Mg3(Si40io)(OH)2), Zincsilite (Zn3(Si40io)(OH)2'4H20), Kerolite ((Mg, Ni)3(Si40io)(OH)2 H20 and mixtures thereof, preferably in an amount equal to or less than 3 wt.%, preferably equal to or less than 2.5 wt.%.

[0276] In particularly preferred embodiments, a first or second composition according to the invention comprises at least one thickener consisting of the component from Beidellit.

[0277] ((Na, Cao 5)o3Al2((Si, AI)40io)(GH)2'4H20), hectorite (Nao 3(Mg, Li)3(Si40 )(F, OH)2), montmorillonite ((Na, Ca)o3(AI, Mg)2(Si40io)(OH)2 nH20), nontronite (Nao 3Fe 3+ 2((Si, AI)40io)(OH)2'4H20), saponite ((Ca, Na)o3(Mg, Fe 2+ )3((Si, AI)40io)(OH)2'4H20), swinefordite

[0278] ((Ca, Na)o3(Al, Li, Mg)2((Si, AI)40io)(OH, F)2-2H20), volkonskoite

[0279] (Cao 3(Cr 3+ , Mg, Fe 3+ )2((Si, AI)40io)(OH)2'4H20), Yakhontovit ((Ca, Na, K)o3(Cu, Fe 2+ , Mg)2(Si40io)(OH)2'3H20), ferrosaponite

[0280] (Cao 3(Fe 2+ , Mg, Fe 3+)3((Si,Al)Si3Oio)(OH)2-4H2O), Sauconite (NaO3Zn3((Si,Al)4Oio)(OH)2-4H2O), Stevensite ((Ca2)O3Mg3(Si4Oio)(OH)2), Zincsilite (Zn3(Si4Oio)(OH)2-4H2O), Kerolite ((Mg,Ni)3(Si4Oio)(OH)2 H2O and mixtures thereof, preferably in an amount of about 0.1 to about 3 wt.%, more preferably about 0.5 to about 2.7 wt.%, further preferably about 0.8 to about 2.4 wt.%, particularly preferably about 1 to about 1.8 wt.%, in particular e.g. 1, 1.05, 1.1, 1.15, 1.2, 1.25, 1.3, 1.35, 1.4, 1.45, 1.5, 1.55, 1.6, 1.65, 1.7, 1.75, 1.8, 1.85, 1.9, 1.95, 2, 2.05, 2.1, 2.15, 2.2, 2.25, 2.3, 2.35, 2.4, 2.45 or 2.5 wt.%, based on the total weight of the first or second composition.

[0281] In particularly preferred embodiments, a first or second composition according to the invention contains at least one thickener, wherein the thickener is hectorite

[0282] (Nao3(Mg, Li)3(Si40io)(F, OH)2) is, preferably in an amount of equal to or less than 3 wt.%, preferably equal to or less than 2.5 wt.%.

[0283] In particularly preferred embodiments, a first or second composition according to the invention contains at least one thickener, wherein the thickener is hectorite

[0284] (NaO3(Mg, Li)3(Si40io)(F, OH)2) is, preferably in an amount of about 0.1 to about 3 wt.%, more preferably about 0.5 to about 2.7 wt.%, further preferably about 0.8 to about 2.4 wt.%, particularly preferably about 1 to about 1.8 wt.%, especially e.g. B. 1, 1.05, 1.1, 1.15, 1.2, 1.25, 1.3, 1.35, 1.4, 1.45, 1.5, 1.55, 1.6, 1.65, 1.7, 1.75, 1.8, 1.85, 1.9, 1.95, 2, 2.05, 2.1, 2.15, 2.2, 2.25, 2.3, 2.35, 2.4, 2.45 or 2.5 wt.%, based on the total weight of the first or second composition.

[0285] In preferred embodiments, a first or second composition according to the invention comprises as the first or second thickener a mixture of xanthan gum and a thickener selected from the group of clay minerals as described herein.

[0286] In preferred embodiments, a first or second composition according to the invention comprises, as the first or second thickener, a mixture of xanthan gum and a thickener selected from the group of clay minerals with the general formula R. 1 aR 2 b(R 3 2cO5c)(OH)d

[0287] up, in the

[0288] R 1 from alkali metal ions, alkaline earth metal ions, H3O + , NH4 and mixtures thereof, preferably alkali and / or alkaline earth metal ions, particularly preferably K + , N / a + , Ca 2+ and mixtures thereof, especially preferred by Na + ,

[0289] a is 0 or any number between 0 and 3, e.g. 0, 0.1, 0.20, 25, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3, preferably any number between 0 and 2; particularly preferably any number between 0 and 1, most particularly preferably 0 or 0.3,

[0290] R 2 selected from alkali, alkaline earth or transition metal ions and mixtures thereof, preferably Mg 2+ , Fe 2+ , Fe 3+ , Ni 2+ , Al 3+ , Li + , Cr, Cu, Zn, V, Mn 2+ , Mn 3+ , Ti and mixtures thereof, especially preferably Mg 2+ , Fe 2+ , Fe 3+ , Ni 2+ , Al 3+ , Li + Cr, Cu, Zn and mixtures thereof, especially Mg 2+ , Li + and mixtures thereof,

[0291] b is any number between 1 and 10, preferably any number between 1 and 3, e.g. 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3; particularly preferably 2 or 3, most particularly preferably 3.

[0292] R 3 from AI, Si, Fe 3+ , Be, Zn, B and mixtures thereof, preferably Si and / or Al, particularly preferably Si,

[0293] c is any number between 1 and 4, preferably 1 or 2; particularly preferably 2,

[0294] R 4 selected from OH, F, O, S and mixtures thereof, preferably OH or F, particularly preferably OH,

[0295] d is any number between 1 and 10, preferably any number between 1 and 4, particularly preferably 1 or 2, most particularly preferably 2,

[0296] where (R 32COSC) is preferably selected from ((Al. Si^Os), ((Al, Si)40 ), (Si20s), (Si40 ) and (AlSisOw), particularly preferably ((Al, Si)40 ) or (Si40 ), particularly preferably (Si40 ), preferably in a total amount of equal to or less than 3 wt.%, preferably equal to or less than 2.5 wt.%.

[0297] In preferred embodiments, a first or second composition according to the invention comprises, as the first or second thickener, a mixture of xanthan gum and a thickener selected from the group of clay minerals with the general formula R. 1 aR 2 b(R 3 2cO5c)(OH)d

[0298] up, in the

[0299] R 1 from alkali metal ions, alkaline earth metal ions, H3O + , NH4 and mixtures thereof, preferably alkali and / or alkaline earth metal ions, particularly preferably K + , N / a + , Ca 2+ and mixtures thereof, especially preferred by Na+ ,

[0300] a is 0 or any number between 0 and 3, e.g. 0, 0.1, 0.20, 25, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3, preferably any number between 0 and 2; particularly preferably any number between 0 and 1, most particularly preferably 0 or 0.3,

[0301] R 2 selected from alkali, alkaline earth or transition metal ions and mixtures thereof, preferably Mg 2+ , Fe 2+ , Fe 3+ , Ni 2+ , Al 3+ , Li + , Cr, Cu, Zn, V, Mn 2+ , Mn 3+ , Ti and mixtures thereof, especially preferably Mg 2+ , Fe 2+ , Fe 3+ , Ni 2+ , Al 3+ , Li + Cr, Cu, Zn and mixtures thereof, especially Mg 2+ , Li + and mixtures thereof,

[0302] b is any number between 1 and 10, preferably any number between 1 and 3, e.g. 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3; particularly preferably 2 or 3, most particularly preferably 3.

[0303] R 3 from AI, Si, Fe 3+ , Be, Zn, B and mixtures thereof, preferably Si and / or Al, particularly preferably Si,

[0304] c is any number between 1 and 4, preferably 1 or 2; particularly preferably 2, R 4 selected from OH, F, O, S and mixtures thereof, preferably OH or F, particularly preferably OH,

[0305] d is any number between 1 and 10, preferably any number between 1 and 4, particularly preferably 1 or 2, most particularly preferably 2,

[0306] where (R 32COSC) is preferably selected from ((Al, Si)2Os), ((Al, Si)40 ), (Si20s), (Si40 ) and (AlSisOw), particularly preferably ((Al, Si)40 ) or (Si40 ), particularly preferably (Si40 ), preferably in a total amount of about 0.1 to about 3 wt.%, preferably about 0.5 to about 2.7 wt.%, further preferably about 0.8 to about 2.4 wt.%, particularly preferably about 1 to about 1.8 wt.%, in particular e.g. B. 1, 1.05, 1.1, 1.15, 1.2, 1.25, 1.3, 1.35, 1.4, 1.45, 1.5, 1.55, 1.6, 1.65, 1.7, 1.75, 1.8, 1.85, 1.9, 1.95, 2, 2.05, 2.1, 2.15, 2.2, 2.25, 2.3, 2.35, 2.4, 2.45 or 2.5 wt.%, based on the total weight of the first or second composition.

[0307] In particularly preferred embodiments, a first or second composition according to the invention contains as a first or second thickener a mixture of xanthan gum and a thickener consisting of Beidellite ((Na, Cao.5)03Al2((Si, Al)40io)(GH)2'4H20), hectorite

[0308] (Nao 3(Mg, Li)3(Si40 )(F, OH)2), montmorillonite ((Na, Ca)o3(Al, Mg)2(Si40io)(GH)2 nH20), nontronite (Nao3Fe 3+ 2((Si, AI)40io)(OH)2'4H20), saponite ((Ca, Na)o3(Mg, Fe 2+ )3((Si, AI)40io)(OH)2'4H20), swinefordite ((Ca, Na)o3(AI, Li, Mg)2((Si, AI)40io)(OH, F)2-2H20), volkonskoite

[0309] (Cao 3(Cr 3+ , Mg, Fe 3+ )2((Si, AI)40io)(OH)2'4H20), Yakhontovit ((Ca, Na, K)o3(Cu, Fe 2+ , Mg)2(Si40io)(CH)2'3H20), ferrosaponite

[0310] (Cao 3(Fe 2+ , Mg, Fe 3+ )3((Si,Al)Si3Oio)(OH)2'4H20), Sauconite (Nao3Zn3((Si,Al)40io)(OH)2-4H20), Stevensite ((Ca2)o3Mg3(Si40io)(OH)2), Zincsilite (Zn3(Si40io)(OH)2'4H20), Kerolite ((Mg, Ni)3(Si40io)(OH)2 H20 and mixtures thereof, preferably in a total amount of 3 wt.% or less, preferably 2.5 wt.% or less.

[0311] In particularly preferred embodiments, a first or second composition according to the invention contains as a first or second thickener a mixture of xanthan gum and a thickener consisting of Beidellite ((Na, Cao.5)03Al2((Si, Al)40io)(GH)2'4H20), hectorite

[0312] (Nao 3(Mg, Li)3(Si40 )(F, OH)2), montmorillonite ((Na, Ca)o3(Al, Mg)2(Si40io)(CH)2 nH20), nontronite (Nao 3Fe 3+ 2((Si, AI)40io)(OH)2'4H20), saponite ((Ca, Na)o 3(Mg, Fe 2+ )3((Si, AI)40io)(OH)2'4H20), swinefordite ((Ca, Na)o3(AI, Li, Mg)2((Si, AI)40io)(OH, F)2-2H20), volkonskoite

[0313] (Cao 3(Cr 3+ , Mg, Fe 3+ )2((Si, AI)40io)(OH)2'4H20), Yakhontovit ((Ca, Na, K)o3(Cu, Fe 2+ , Mg)2(Si40io)(CH)2'3H20), ferrosaponite

[0314] (Cao 3(Fe 2+ , Mg, Fe 3+)3((Si,Al)Si3Oio)(OH)2'4H2O), Sauconite (NaO3Zn3((Si,Al)4Oio)(OH)2-4H2O), Stevensite ((Ca2)o3Mg3(Si4Oio)(OH)2), Zincsilite (Zn3(Si4Oio)(OH)2'4H2O), Kerolite ((Mg,Ni)3(Si4Oio)(OH)2 H2O and mixtures thereof, preferably in a total amount of about 0.1 to about 3 wt.%, more preferably about 0.5 to about 2.7 wt.%, further preferably about 0.8 to about 2.4 wt.%, particularly preferably about 1 to about 1.8 wt.%, in particular e.g. 1, 1.05, 1.1, 1.15, 1.2, 1.25, 1.3, 1.35, 1.4, 1.45, 1.5, 1.55, 1.6, 1.65, 1.7, 1.75, 1.8, 1.85, 1.9, 1.95, 2, 2.05, 2.1, 2.15, 2.2, 2.25, 2.3, 2.35, 2.4, 2.45 or 2.5 wt.%, based on the total weight of the first or second composition.

[0315] In particularly preferred embodiments, a first or second composition according to the invention contains as a first or second thickener a mixture of xanthan gum and hectorite (Nao 3(Mg, Li)3(Si4O )(F, OH)2), preferably in a total amount of equal to or less than 3 wt.%, preferably equal to or less than 2.5 wt.%.

[0316] In particularly preferred embodiments, a first or second composition according to the invention contains as a first or second thickener a mixture of xanthan gum and hectorite (NaO 3(Mg, Li)3(Si40 )(F, OH)2), preferably in a total amount of about 0.1 to about 3 wt.%, more preferably about 0.5 to about 2.7 wt.%, further preferably about 0.8 to about 2.4 wt.%, more preferably about 1 to about 1.8 wt.%, in particular e.g. B. 1, 1.05, 1.1, 1.15, 1.2, 1.25, 1.3, 1.35, 1.4, 1.45, 1.5, 1.55, 1.6, 1.65, 1.7, 1.75, 1.8, 1.85, 1.9, 1.95, 2, 2.05, 2.1, 2.15, 2.2, 2.25, 2.3, 2.35, 2.4, 2.45 or 2.5 wt.%, based on the total weight of the first or second composition.

[0317] In preferred embodiments, the total amount of the first or second thickener used can be reduced to as little as 1.5 wt.% when a combination of xanthan gum and clay mineral, as described herein, is used.

[0318] The first and second thickener or thickener mixture according to the invention has proven to be advantageous for the stability, in particular for the storage stability, of the first and / or second composition according to the invention.

[0319] The first and second thickener or thickener mixture according to the invention has proven to be advantageous for the coloring properties of the first and / or second composition according to the invention.

[0320] In further embodiments, a first and / or second composition according to the invention can be added to the first and second thickener according to the invention.

[0321] The thickener mixture shall contain at least one further thickener, preferably selected from polysaccharides which may be chemically and / or physically modified, in particular celluloses or microcrystalline celluloses, cellulose ethers of Ci-4 alcohols or cellulose esters, in particular methylcelluloses, ethylcelluloses, hydroxyalkylcelluloses (e.g. hydroxyethylcellulose), methylhydroxyalkylcelluloses and carboxymethylcelluloses (e.g. cellulose gum); xanthan gum;

[0322] Scleroglucan gum; diutan gum; alginic acids and / or alginates; agar-agar and / or agarose; starch and starch derivatives such as amylose, amylopectin, and dextrins; karaya gum; locust bean gum; gum arabic; pectins; dextrans and guar gum; clay minerals; and mixtures and salts thereof. Thickeners made from polymers of acrylic acid (carbomer / polyacrylic acid) are also suitable, such as thickeners based on polyacrylic acid, polyacrylates, polyacrylamides, polyvinylpyrrolidone, polyvinyl alcohol, polyurethanes, styrene-maleic anhydride copolymers, polyethylene glycols, and hydrophobically modified polyethers. Other ingredients

[0323] A first and / or second composition according to the invention may optionally contain preservatives, organic solvents, water and other suitable ingredients.

[0324] In preferred embodiments, a first and / or second composition according to the invention may contain at least one preservative. The preservative is preferably selected from levulinic acid, pentanediol, 1,2-octanediol (INCI: caprylyl glycol).

[0325] 2-Phenoxyethanol, 4-Hydroxybenzoic acid methyl ester, 4-Hydroxybenzoic acid propyl ester, Benzyl alcohol, Benzyl alcohol, 1-Phenoxypropan-2-ol, Isopropanol, Ethanol, Zinc pyrithione, Benzoic acid, especially sodium benzoate (INCI Sodium Benzoate), Salicylic acid, Sorbic acid, Formic acid, Propionic acid, 2-Hydroxydiphenyl, 4-Hydroxybenzoic acid, Dehydroacetic acid, Dibromohexamidine, 10-Undecylenic acid, Hexetidine, Trichlorocarban, Triclosanum, 4-Chloro-3,4-dimethylphenol, Imidazolidinyl urea, Hexamethylenetetramine, 1-(4-Chlorphenoxy)-1-(imidazol-1-yl)-3,3-dimethyl-2-butanone 1,3-bis-(hydroxymethyl)-5,5-dimethyl-2,4-imidazolidinedione, 1-hydroxy-4-methyl-6-(2,4,4-trimethylpentyl)-2-pyridone, bromochlorophene, 3-methyl-4-(1-methylethyl)phenol, 5-chloro-2-methyl-3(2H)-isothiazolone, 2-Benzyl-4-chlorophenol, 2-chloroacetamide, chlorhexidine, 4,4-dimethyl-1,3-oxazolidine, 1-phenoxypropan-2-ol, hexamidinum, 5-ethyl-1-aza-3,7-dioxabicyclo-[3.3.0]-octane, Chlorphenesin, Sodium hydroxymethylaminoacetate, Benzylhemiformal, 3-iod-2-propynyl butylcarbamate, Methylisothiazolinone and Ethyllauroylarginate.

[0326] In preferred embodiments, a first and / or second composition according to the invention can contain, based on the total weight of the first or second composition, at least one preservative in an amount of about 0.1 to about 2 wt.%, preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.3 to about 1 wt.%.

[0327] In particularly preferred embodiments, a first and / or second composition according to the invention may contain at least one preservative, wherein the preservative is selected from 1,2-octanediol (INCI Caprylyl Glycol), sodium benzoate (INCI Sodium Benzoate) and mixtures thereof.

[0328] In particularly preferred embodiments, a first and / or composition according to the invention, based on the total weight of the first or second composition, may contain at least one preservative in an amount of about 0.1 to about 2 wt.%, preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.3 to about 1 wt.%, wherein the preservative is selected from 1,2-octanediol (INCI Caprylyl Glycol), sodium benzoate (INCI Sodium Benzoate) and mixtures thereof.

[0329] In particularly preferred embodiments, a first and / or second composition according to the invention can contain, based on the total weight of the first or second composition, at least one preservative in an amount of about 0.1 to about 2 wt.%, preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%, particularly preferably about 0.5 to about 1 wt.%, wherein the preservative is 1,2-octanediol (INCI Caprylyl Glycol).

[0330] In particularly preferred embodiments, a first and / or second composition according to the invention can contain, based on the total weight of the first or second composition, at least one preservative in an amount of about 0.1 to about 2 wt.%, preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%, particularly preferably about 0.3 to about 0.5 wt.%, wherein the preservative is sodium benzoate (INCI Sodium Benzoate).

[0331] In preferred embodiments, a first and / or second composition according to the invention may contain water. The water essentially serves as a solvent for the ingredients of the first or second composition. If dried natural dye or...

[0332] When plant parts, powders or extracts containing natural dye precursors are used, they are preferably dispersed in water.

[0333] In preferred embodiments, a first composition according to the invention may contain water in an amount of more than 45 wt.%, preferably more than 50 wt.%, further preferably more than 60 wt.%, particularly preferably more than 70 wt.% and most preferably up to 75 wt.%, in particular 65, 66, 67, 68, 69, 70, 71, 72, 73, 74 or 75 wt.%, based on the total weight of the first composition.

[0334] In preferred embodiments, a second composition according to the invention may contain water in an amount of more than 40 wt.%, preferably more than 45 wt.%, further preferably more than 50 wt.%, particularly preferably more than 60 wt.% and most preferably up to 65 wt.%, in particular 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64 or 65 wt.%, based on the total weight of the second composition.

[0335] In preferred embodiments, a first and / or second composition according to the invention can contain an organic solvent, wherein the organic solvent preferably consists of C1-4 alkanols and C 2-4-Polyols, in particular ethanol, isopropanol, n-propanol, ethylene glycol, 1,2-propanediol, glycerol and 1,3-butylene glycol, and mixtures thereof, are selected, preferably in an amount of about 2 to about 50 wt.%, more preferably about 4 to about 50 wt.%, further preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%, based on the total weight of the first or second composition.

[0336] In particularly preferred embodiments, a first and / or second composition according to the invention can contain 1,2-propanediol in an amount of about 2 to about 50 wt.%, preferably about 4 to about 50 wt.%, more preferably about 5 to about 30 wt.%, and particularly preferably about 10 to about 25 wt.%, based on the total weight of the first or second composition.

[0337] In preferred embodiments, a first and / or second composition according to the invention can, alternatively or additionally, contain at least one further organic solvent, wherein the organic solvent preferably has a phenyl group in the molecule. This solvent is preferably selected from phenoxyethanol, benzyl alcohol, and mixtures thereof. It has been surprisingly found that such aromatic solvents can have a positive effect on the staining results. In preferred embodiments, a first and / or second composition according to the invention can contain the organic solvent, based on the total weight of the first or second composition, in an amount of about 0.1 to about 3 wt.%, preferably about 0.5 to about 2.5 wt.%, and more preferably about 0.8 to about 1 wt.%. In a preferred embodiment, a first and / or second composition according to the invention can be free of further organic solvents.

[0338] If the first or second thickener (i) is a mixture of xanthan gum and scleroglucan gum or (ii) diutan gum, then a first and / or second composition according to the invention can be substantially free of organic solvents, preferably free of organic solvents. "Substantially free of" in this context means that the composition contains less than 1 wt.%, preferably less than 0.5 wt.%, more preferably less than 0.1 wt.%, and particularly preferably less than 0.01 wt.%, organic solvent, based on the total weight of the composition.

[0339] In preferred embodiments, a first and / or second composition according to the invention is water-based. In preferred embodiments, a first and / or second composition according to the invention is an aqueous solution or a gel, or the first and / or second composition is an emulsion, preferably an O / W emulsion or a W / O emulsion. In particularly preferred embodiments, a first and / or second composition according to the invention is an aqueous solution or a gel, in particular a gel.

[0340] In preferred embodiments, the pH value of a first composition according to the invention is adjusted to a value of about 3 to about 8, preferably about 3.5 to about 7.5, more preferably about 4 to about 7, particularly preferably about 4 to about 6.6, in each case measured at 20°C.

[0341] In preferred embodiments, the pH value of the second composition according to the invention is adjusted to a value of about 2.5 to about 10, preferably about 3 to about 9.5, more preferably about 3.5 to about 9.5, particularly preferably about 4 to about 9, in each case measured at 20°C.

[0342] In preferred embodiments, the pH value of a first and / or second composition according to the invention is adjusted using an acid or a base. Preferred acids are selected from citric acid, lactic acid, gluconic acid, acetic acid, propionic acid, oxalic acid, malonic acid, succinic acid, glutaric acid, galactaric acid (mucus acid), tartaric acid, malic acid, sulfuric acid, and phosphoric acid, and mixtures thereof, with citric acid being particularly preferred.

[0343] Preferred bases are sodium hydroxide, potassium hydroxide, arginine, lysine, monoethanolamine, triethanolamine, 2-amino-2-methylpropan-1-ol, and mixtures thereof, particularly preferably sodium hydroxide. In preferred embodiments, the desired pH is adjusted using a buffer system selected from a mixture of a moderately strong or weak acid with its conjugate or corresponding base (or the respective salt) and a mixture of a moderately strong or weak base with its conjugate or corresponding acid. Suitable buffer systems are known to those skilled in the art.

[0344] In preferred embodiments, a first composition according to the invention is essentially free of enzymes. "Essentially free of enzymes" means that the first composition contains less than 0.1 wt.%, preferably less than 0.05 wt.%, more preferably less than 0.01 wt.%, and particularly preferably less than 0.001 wt.%, enzyme. In particularly preferred embodiments, a first composition according to the invention is free of enzymes. In particular, a first composition according to the invention is essentially free of hydrolases, especially cellulases and glucosidases. In particularly preferred embodiments, a first composition according to the invention is free of hydrolases, especially cellulases (EC 3.2.1.4) and glucosidases (EC 3.2.1.21).

[0345] In particularly preferred embodiments, a first composition according to the invention is free of enzymes, i.e., a first composition according to the invention does not contain any enzymes that originate from the plant parts, powders, or extracts used.

[0346] Table 1: Inventive implementation forms 1-20

[0347] 1 2 3 4 5 6 7 8 Color source

[0348] Glycosylated 0.1-25 0.2-15 0.3-5 0.4-1.4 0.1-25 0.2-15 0.3-5 0.4-1.4 Dye

[0349] Microcrystalline 1-12 2-10 2.5-9 3-8 1-12 2-10 2.5-9 3-8 Cellulose

[0350] Preservatives 0.1-2 0.1-1, 5 0.3-1 0.3-1 0.1-2 0.1-1, 5 0.3-1 0.3-1 Organic 4-50 5-30 10-25 10-25 4-50 5-30 10-25 10-25 Solvents,

[0351] preferably 1,2-propanediol

[0352] Water Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Enzymqel

[0353] ß-Glucosidase 0.001-2.5 0.01-2 0.1-1, 5 0.5-1 0.001-2.5 0.01-2 0.1-1.5 0.5-1 Xanthan gum / 0.1-3 0.5-2, 7 0.8-2, 4 1-1.8

[0354] Scleroglucan Gum

[0355] Diutan Gum 0.1-3 0.25-2.5 0.5-1.5 0.5-1 Preservative 0.1-2 0.1-1.5 0.3-1 0.3-1 0.1-2 0.1-1.5 0.3-1 0.3-1

[0356]

[0357] Organic < 8 0.1-6 0.5-4 0.5-4 < 8 0.1-6 0.5-4 0.5-4 Solvent

[0358] Water Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100

[0359] 9 10 11 12 13 14 15 16 Color gel

[0360] Glycosylated 0.1-25 0.2-15 0.3-5 0.4-1.4 0.1-25 0.2-15 0.3-5 0.4-1.4 Dye

[0361] Microcrystalline 1-12 2-10 2.5-9 3-8 1-12 2-10 2.5-9 3-8 Cellulose

[0362] Preservatives 0.1-2 0.1-1, 5 0.3-1 0.3-1 0.1-2 0.1-1, 5 0.3-1 0.3-1 Organic 4-50 5-30 10-25 10-25 4-50 5-30 10-25 10-25 Solvents,

[0363] preferably 1,2-propanediol

[0364] Water Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Enzymqel

[0365] ß-glucosidase 0.001-2.5 0.01-2 0.1-1, 5 0.5-1 0.001-2.5 0.01-2 0.1-1.5 0.5-1 clay mineral, 0.1-3 0.5-2, 7 0.8-2, 4 1-1.8

[0366] preferably hectorite

[0367] Xanthan gum / 0.1-3 0.5-2, 7 0.8-2, 4 1-1.8 clay mineral,

[0368] preferably hectorite

[0369] Preservatives 0.1-2 0.1-1, 5 0.3-1 0.3-1 0.1-2 0.1-1, 5 0.3-1 0.3-1 Organic 2-14 4-12 6-10 6-10 2-14 4-12 6-10 6-10 Solvents,

[0370] preferably 1,2-propanediol

[0371] Water Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100

[0372] 17 18 19 20

[0373] Color gel

[0374] Glycosylated 0.1-25 0.2-15 0.3-5 0.4-1.4

[0375] dye

[0376] Microcrystalline 1-12 2-10 2.5-9 3-8

[0377] Cellulose

[0378] Preservatives 0.1-2 0.1-1.5 0.3-1 0.3-1

[0379]

[0380] Organic 4-50 5-30 10-25 10-25

[0381] Solvent,

[0382] preferably 1,2-propanediol

[0383] Water Ad 100 Ad 100 Ad 100 Ad 100

[0384] Enzyme source

[0385] ß-Glucosidase 0.001-2.5 0.01-2 0.1-1, 5 0.5-1

[0386] Microcrystalline 1-12 2-10 2.5-9 3-8

[0387] Cellulose

[0388] Preservatives 0.1-2 0.1-1.5 0.3-1 0.3-1

[0389] Organic 4-50 5-30 10-25 10-25

[0390] Solvent,

[0391] preferably 1,2-propanediol

[0392] Water Ad 100 Ad 100 Ad 100 Ad 100

[0393]

[0394] Values ​​given in wt.% based on active ingredient content and total weight of the color gel or enzyme gel.

[0395] Table 2: Inventive implementation forms 21-40

[0396] 21 22 23 24 25 26 27 28 color qel

[0397] Glycosylated 0.1-25 0.2-15 0.3-5 0.4-1.4 0.1-25 0.2-15 0.3-5 0.4-1.4 Dye

[0398] Xanthan gum / 0.1-3 0.5-2, 7 0.8-2, 4 1-1.8

[0399] Scleroglucan Gum

[0400] Diutan Gum 0.1-3 0.25-2.5 0.5-1.5 0.5-1 Preservative 0.1-2 0.1-1.5 0.3-1 0.3-1 0.1-2 0.1-1.5 0.3-1 0.3-1 Organic < 8 0.1-6 0.5-4 0.5-4 < 8 0.1-6 0.5-4 0.5-4 Solvent

[0401] Water Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Enzymqel

[0402] ß-Glucosidase 0.001-2.5 0.01-2 0.1-1, 5 0.5-1 0.001-2.5 0.01-2 0.1-1.5 0.5-1 Microcrystalline 1-12 2-10 2.5-9 3-8 1-12 2-10 2.5-9 3-8 Cellulose

[0403] Preservatives 0.1-2 0.1-1, 5 0.3-1 0.3-1 0.1-2 0.1-1, 5 0.3-1 0.3-1 Organic 4-50 5-30 10-25 10-25 4-50 5-30 10-25 10-25 Solvents,

[0404]

[0405] preferably 1,2-propanediol

[0406] Water Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100

[0407] 29 30 31 32 33 34 35 36 Color gel

[0408] Glycosylated 0.1-25 0.2-15 0.3-5 0.4-1.4 0.1-25 0.2-15 0.3-5 0.4-1.4 Dye

[0409] Clay mineral, 0.1-3 0.5-2, 7 0.8-2, 4 1-1.8

[0410] preferably hectorite

[0411] Xanthan gum / 0.1-3 0.5-2, 7 0.8-2, 4 1-1.8 clay mineral,

[0412] preferably hectorite

[0413] Preservatives 0.1-2 0.1-1, 5 0.3-1 0.3-1 0.1-2 0.1-1, 5 0.3-1 0.3-1 Organic 2-14 4-12 6-10 6-10 2-14 4-12 6-10 6-10 Solvents,

[0414] preferably 1,2-propanediol

[0415] Water Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Enzymqel

[0416] ß-Glucosidase 0.001-2.5 0.01-2 0.1-1, 5 0.5-1 0.001-2.5 0.01-2 0.1-1.5 0.5-1 Microcrystalline 1-12 2-10 2.5-9 3-8 1-12 2-10 2.5-9 3-8 Cellulose

[0417] Preservatives 0.1-2 0.1-1, 5 0.3-1 0.3-1 0.1-2 0.1-1, 5 0.3-1 0.3-1 Organic 4-50 5-30 10-25 10-25 4-50 5-30 10-25 10-25 Solvents,

[0418] preferably 1,2-propanediol

[0419] Water Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100 Ad 100

[0420] 37 38 39 40

[0421] Color gel

[0422] Glycosylated 0.1-25 0.2-15 0.3-5 0.4-1.4

[0423] dye

[0424] Microcrystalline 1-12 2-10 2.5-9 3-8

[0425] Cellulose

[0426] Preservatives 0.1-2 0.1-1.5 0.3-1 0.3-1

[0427]

[0428] Organic 4-50 5-30 10-25 10-25

[0429] Solvent,

[0430] preferably 1,2-propanediol

[0431] Water Ad 100 Ad 100 Ad 100 Ad 100

[0432] Enzyme source

[0433] ß-Glucosidase 0.001-2.5 0.01-2 0.1-1, 5 0.5-1

[0434] Microcrystalline 1-12 2-10 2.5-9 3-8

[0435] Cellulose

[0436] Preservatives 0.1-2 0.1-1.5 0.3-1 0.3-1

[0437] Organic 4-50 5-30 10-25 10-25

[0438] Solvent,

[0439] preferably 1,2-propanediol

[0440] Water Ad 100 Ad 100 Ad 100 Ad 100

[0441]

[0442] Values ​​given in wt.% based on active ingredient content and total weight of the color gel or enzyme gel.

[0443] Application mixture and process for non-oxidative dyeing of keratin fibers

[0444] In preferred embodiments, the first composition and the second composition are kept separate until use. This is particularly advantageous so that the glycosylated dye, especially a glycosylated natural dye, in the first composition is only converted into its active form shortly before use by means of the β-glucosidase in the second composition.

[0445] In preferred embodiments, an application mixture according to the invention is produced by mixing a first composition according to the invention with a second composition according to the invention.

[0446] By mixing the first composition and the second composition, the glycosylated dye, in particular glycosylated natural dye, and the β-glucosidase come into contact in an aqueous environment, and the β-glucosidase can cleave the glucose residue from the glycosylated dye, in particular glycosylated natural dye, wherein the glycosylated dye, in particular glycosylated natural product, is selected from glycosylated anthocyanins, glycosylated flavones / flavonoids (in particular luteolin, apigenin, kaempferol, flavanomarein), glycosylated naphthoquinones and mixtures, in particular selected from indican, hennoside (glycosylated lawsone), sennoside and apigenin glucoside. The mixture thus obtained with the activated dye, in particular activated natural dye, is the ready-to-use dye, which can then be applied to the keratinous fibers to be dyed, especially human hair.Another object of the present invention therefore relates to a kit for non-oxidative dyeing of keratinous fibers, in particular human hair, comprising the following components which are physically separate from each other:.

[0447] (A) a first composition, wherein the first composition comprises, each based on the total weight of the first composition,

[0448] a) at least one glycosylated dye, in particular a glycosylated natural dye, preferably in an amount of about 0.1 to about 25 wt.%, more preferably about 0.2 to about 15 wt.%, further preferably about 0.3 to about 2 wt.%, particularly preferably about 0.4 to about 1.4 wt.%, b) at least one first thickener, preferably in an amount of about 0.1 to about 12 wt.%, more preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%,

[0449] c) optionally at least one preservative, preferably in an amount of about 0.1 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%, and

[0450] d) at least one organic solvent, preferably in an amount of about 2 to about 50 wt.%, more preferably about 4 to about 50 wt.%, further preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%, wherein the organic solvent consists of C1-4-alkanols and C 2-4 -Polyols, in particular ethanol, isopropanol, n-propanol, ethylene glycol, 1,2-propanediol, glycerol and 1,3-butylene glycol, and mixtures thereof;

[0451] e) Water, preferably in an amount of more than 45 wt.%, preferably more than 50 wt.%, further preferably more than 60 wt.%, particularly preferably more than 70 wt.% and most particularly preferably up to 75 wt.%; and

[0452] (B) a second composition, wherein the second composition comprises, each in relation to the total weight of the second composition,

[0453] a) at least one β-glucosidase (EC 3.2.1.21), preferably in an amount of about 0.001 to about 2.5 wt.%, more preferably about 0.01 to about 2 wt.%, further preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.5 to about 1 wt.%, based on active enzyme protein, b) at least one second thickener, preferably in an amount of about 0.1 to about 12 wt.%, more preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%,

[0454] c) optionally at least one preservative, preferably in an amount of about 0.1 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%,

[0455] d) at least one organic solvent, preferably in an amount of about 2 to about 50 wt.%, more preferably about 4 to about 50 wt.%, further preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%, wherein the organic solvent consists of C1-4-alkanols and C 2-4 -polyols, in particular ethanol, isopropanol, n-propanol, ethylene glycol, 1,2-propanediol, glycerol and 1,3-butylene glycol, and mixtures thereof; e) water, preferably in an amount of more than 40 wt.%, more preferably more than 45 wt.%, further preferably more than 50 wt.%, particularly preferably more than 60 wt.% and most particularly preferably up to 65 wt.%,

[0456] where the first thickener and / or the second thickener is microcrystalline cellulose.

[0457] In further embodiments, a means according to the invention can also comprise a third or more component(s).

[0458] Another object of the present invention is a method for non-oxidative dyeing of keratinous fibers, in particular human hair, comprising the following process steps in the specified order

[0459] (a) Providing a first composition, the first composition comprising, each in relation to the total weight of the first composition,

[0460] a) at least one glycosylated dye, in particular a glycosylated natural dye, preferably in an amount of about 0.1 to about 25 wt.%, more preferably about 0.2 to about 15 wt.%, further preferably about 0.3 to about 2 wt.%, particularly preferably about 0.4 to about 1.4 wt.%, b) at least one first thickener, preferably in an amount of about 0.1 to about 12 wt.%, more preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%,

[0461] c) optionally at least one preservative, preferably in an amount of about 0.1 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%, and

[0462] d) at least one organic solvent, preferably in an amount of about 2 to about 50 wt.%, more preferably about 4 to about 50 wt.%, further preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%, wherein the organic solvent consists of C1-4-alkanols and C 2-4 -Polyols, in particular ethanol, isopropanol, n-propanol, ethylene glycol, 1,2-propanediol, glycerol and 1,3-butylene glycol, and mixtures thereof;

[0463] e) Water, preferably in an amount of more than 45 wt.%, preferably more than 50 wt.%, further preferably more than 60 wt.%, particularly preferably more than 70 wt.% and most particularly preferably up to 75 wt.%; and

[0464] (b) Providing a second composition, the second composition comprising, in each case, based on the total weight of the second composition,

[0465] a) at least one β-glucosidase (EC 3.2.1.21), preferably in an amount of about 0.001 to about 2.5 wt.%, more preferably about 0.01 to about 2 wt.%, further preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.5 to about 1 wt.%, based on active enzyme protein; b) at least one second thickener, preferably in an amount of about 0.1 to about 12 wt.%, more preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%; c) optionally at least one preservative, preferably in an amount of about 0.1 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.3 to about 1 wt.%.

[0466] d) at least one organic solvent, preferably in an amount of about 2 to about 50 wt.%, more preferably about 4 to about 50 wt.%, further preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%, wherein the organic solvent consists of C1-4-alkanols and C 2-4 -Polyols, in particular ethanol, isopropanol, n-propanol, ethylene glycol, 1,2-propanediol, glycerol and 1,3-butylene glycol, and mixtures thereof;

[0467] e) Water, preferably in an amount of more than 40 wt.%, preferably more than 45 wt.%, further preferably more than 50 wt.%, particularly preferably more than 60 wt.% and most particularly preferably up to 65 wt.%;

[0468] wherein the first thickener and / or the second thickener is microcrystalline cellulose;

[0469] (c) Mixing the first composition from step (a) with the second composition from step (b) to produce a ready-to-use dye, preferably in a ratio of 15:1 to 3:1, more preferably 12:1 to 5:1, more preferably 10:1 to 7:1, particularly preferably 9:1,

[0470] (d) optionally incubate the dye from step (c) for 0 to about 45 minutes, preferably about 5 minutes to about 30 minutes, preferably about 10 minutes to about 20 minutes,

[0471] (e) Applying the dye obtained in step (c) to the keratin fibers, wherein the application of the dye obtained in step (c) to the keratin fibers takes place preferably within about 1 second to about 30 minutes, preferably within about 10 seconds to about 15 minutes after completion of step (c) or (d),

[0472] (f) Allowing the dye to act on the keratinous fibers, wherein the acting time is preferably about 30 seconds to about 90 minutes, more preferably about 5 minutes to about 60 minutes, more preferably about 15 minutes to about 45 minutes, and / or wherein the acting preferably takes place at a temperature of about 20°C to about 40°C, more preferably about 25°C to about 40°C, more preferably about 25°C to about 37°C, in particular 25°C, 26°C, 27°C, 28°C, 29°C, 30°C, 31°C, 32°C, 33°C, 34°C, 35°C, 36°C or 37°C, most preferably about 37°C,

[0473] (g) Rinsing the keratinous fibers with water, and

[0474] (h) optional drying of the keratinous fibers.

[0475] In preferred embodiments, a ready-to-use dye is produced from the first composition according to the invention, as described herein, and the second composition according to the invention, as described herein, by mixing the first composition and the second composition together. In preferred embodiments, a ready-to-use dye is produced from the first composition according to the invention, as described herein, and the second composition according to the invention, as described herein, by mixing the first composition and the second composition together in a ratio of first to second composition of 15:1 to 3:1, preferably 12:1 to 5:1, more preferably 10:1 to 7:1, particularly preferably 9:1, especially 10:1, 9.5:1, 9:1, 8.5:1, 8:1 or 7:1. The mixing of the first composition and the second composition is carried out by methods customary in the trade, e.g.Mixing one of the two compositions with a suitable stirring agent. A suitable stirring agent may be, for example, a brush, spoon, or spatula with which the first composition and the second composition can be mixed in a bowl or on a plate (or a similar suitable container). Alternatively, the first composition and the second composition may also be mixed using a shaker bottle. In preferred embodiments, a ready-to-use dye may contain any embodiment of the first composition and / or the second composition described herein. Reference is made to the relevant sections of the description.

[0476] According to the invention, "ready-to-use dye" means the dye obtained by mixing all the components contained in the dyeing kit according to the invention and intended for and used for immediate application to keratinous fibers, in particular human hair. According to the invention, "immediately" in this context encompasses a period of approximately 1 second to approximately 30 minutes. The ready-to-use dye is applied to the keratinous fibers, in particular human hair. Preferably, the application of the ready-to-use dye to the keratinous fibers, in particular human hair, takes place within approximately 1 second to approximately 30 minutes, and more preferably within approximately 10 seconds to approximately 15 minutes, after preparation of the ready-to-use dye.

[0477] In preferred embodiments, the keratinous fibers onto which the ready-to-use dye is applied are dry.

[0478] In preferred embodiments, the ready-to-use dye is incubated at room temperature for 0 to about 45 minutes, preferably about 5 minutes to about 30 minutes, and more preferably about 10 minutes to about 20 minutes. The application of the ready-to-use dye to the keratinous fibers, particularly human hair, then takes place within about 1 second to about 30 minutes, and more preferably within about 10 seconds to about 15 minutes, after completion of the incubation of the ready-to-use dye.

[0479] It was surprisingly discovered that the development of the dyeing result on keratinous fibers, particularly human hair, can be further accelerated if the ready-to-use dye according to the invention is applied under the influence of heat, e.g., by a heat lamp, a hair dryer, or a hairdryer. In preferred embodiments, a dyeing process according to the invention is characterized in that the ready-to-use dye is applied under the influence of heat. In preferred embodiments, a dyeing process according to the invention is characterized in that the ready-to-use dye is applied at a temperature of approximately 25°C to approximately 60°C, preferably approximately 30°C to approximately 50°C, more preferably approximately 35°C to approximately 40°C, particularly preferably approximately 30°C to approximately 40°C, and most preferably approximately 37°C.In preferred embodiments, a dyeing process according to the invention is characterized in that the contact time of the ready-to-use dye on the keratinous fibers, in particular human hair, is approximately 30 seconds to approximately 90 minutes, preferably approximately 5 minutes to approximately 60 minutes, more preferably approximately 15 minutes to approximately 45 minutes.

[0480] In preferred embodiments, a dyeing process according to the invention is characterized in that the application of the ready-to-use dyeing agent takes place at a temperature of about 25°C to about 60°C, preferably about 30°C to about 50°C, more preferably about 35°C to about 40°C, particularly preferably about 30°C to about 40°C, and most preferably about 37°C, and that the application time of the ready-to-use dyeing agent on the keratinous fibers, in particular human hair, is about 30 seconds to about 90 minutes, preferably about 5 minutes to about 60 minutes, more preferably about 15 minutes to about 45 minutes.

[0481] After the exposure time has elapsed, the keratinous fibers, especially human hair, are rinsed with water to wash out the dye.

[0482] In preferred embodiments, the keratin fibers, particularly human hair, are dried after rinsing out the dye. In preferred embodiments, drying can be carried out without the active application of heat, particularly using an absorbent cloth, e.g., a towel. The towel-dried hair can optionally be partially or completely dried with a hairdryer or other heat source. Allowing the keratin fibers to air dry is also possible. In preferred embodiments, drying can be carried out with the application of heat at a temperature of approximately 25°C to approximately 120°C, preferably approximately 30°C to approximately 80°C, and more preferably approximately 35°C to approximately 60°C. In preferred embodiments, the heat is applied using a heat lamp, a drying rod, a hair dryer hood, a flat iron, or a hairdryer.

[0483] In conventional dyeing agents and dyeing processes using natural dyes, compounds, especially salts, of metals other than alkali metals are frequently used to improve the adhesion of the natural dye to the keratinous fibers. Such compounds are also referred to as "mordanting agents." US 2004 / 0055096 A1, for example, discloses a pretreatment or posttreatment of keratinous fibers with metals or metal salts selected from aluminum, iron, zinc, nickel, calcium, and magnesium. Other mordants include the transition metals and lanthanides and their salts and complex compounds.

[0484] The content of metal ions other than alkali metal ions, which is typically present in the water used, e.g., tap water or municipal water, is disregarded here. For example, tap water can contain an average of 2 mg of copper ions per liter, i.e., approximately 0.0002 wt% copper ions. Tap water contains approximately 20 to approximately 120 mg of calcium ions per liter, i.e., approximately 0.002 to approximately 0.012 wt% calcium ions. Tap water contains approximately 2 to approximately 25 mg of magnesium ions per liter, i.e., approximately 0.0002 to approximately 0.0025 wt% magnesium ions. At such low concentrations, no perceptible pickling effect occurs. For the purposes of the present invention, compositions and agents according to the invention are "free of pickling agents" if the pickling agent(s) is / are present at maximum concentrations that are naturally typical for the ingredients used, such as water, enzymes, and plant parts.

[0485] The dyes, dye kits, and dyeing processes according to the invention can do without the use of such mordants. In particularly preferred embodiments, the dyes and dyeing processes according to the invention are therefore characterized in that they are free of mordants.

[0486] In order to preserve the hair-friendly potential of natural dyes, preferred methods according to the invention are limited to those methods in which the keratinous fibers, in particular human hair, have not been or are not treated with a mordant for a period of up to 7 days before and up to 7 days after the application of a dye according to the invention.

[0487] In preferred embodiments, compositions, dyes, dye kits, and dyeing processes according to the invention are characterized in that they are essentially free of oxidizing agents. In the context of the invention, "essentially free of oxidizing agents" means that oxidizing agents other than atmospheric oxygen are used in the compositions, agents, kits, and processes according to the invention in an amount of no more than 1% by weight, preferably no more than 0.5% by weight, more preferably no more than 0.1% by weight, and particularly preferably no more than 0.01% by weight, wherein the oxidizing agents other than atmospheric oxygen are selected from the group consisting of hydrogen peroxide, persulfates, perbromates, percarbonates, perborates, and percarbamides. Atmospheric oxygen does not constitute an oxidizing agent in the context of the invention.In particularly preferred embodiments, compositions, dyes, dye kits, and dyeing processes according to the invention are characterized in that they are free of oxidizing agents, i.e., that no oxidizing agents other than atmospheric oxygen are used in the compositions, agents, kits, and processes according to the invention. Specifically excluded are the oxidizing agents hydrogen peroxide, persulfates, perbromates, percarbonates, perborates, and percarbamides. Atmospheric oxygen does not constitute an oxidizing agent in the context of the invention.

[0488] In order to preserve the hair-friendly potential of natural dyes, preferred methods according to the invention are limited to those methods in which the keratinous fibers, in particular human hair, have not been or are not treated with an oxidizing agent for a period of up to 7 days before and up to 7 days after the application of a dye according to the invention.

[0489] To preserve the hair-friendly potential of natural dyes, preferred methods according to the invention are limited to those in which the keratinous fibers, in particular human hair, have not been treated with a keratin-reducing compound for a period of up to 7 days before and up to 7 days after the application of a dye according to the invention. In preferred embodiments, compositions, dyes, dyeing kits, and dyeing methods according to the invention are characterized in that they are essentially free of, preferably free of, oxidation dye precursors, i.e., that no oxidation dye precursors are used in the compositions, agents, kits, and methods according to the invention.Typische Oxidationsfarbstoffvorprodukte umfassen p-Aminophenol, 4-Amino-3-methylphenol, 4-Amino-2-aminomethylphenol, 4-Amino-2-(1,2-dihydroxyethyl)phenol, 4-Amino-2-(diethylaminomethyl)phenol, 2-(2,5-Diaminophenyl)ethanol, 2-(1,2-Dihydroxyethyl)-p-phenylendiamin, N, N-Bis-(2-hydroxyethyl)-p-phenylendiamin, N-(4-Amino-3-methylphenyl)-N-[3-(1 H-imidazol-1-yl)propyl]amin, N, N'-Bis-(2-hydroxyethyl)-N, N'-bis-(4-aminophenyl)-1,3-diaminopropan-2-ol, Bis-(2-hydroxy-5-aminophenyl)methan, 1.3-Bis-(2,5-diaminophenoxy)propan-2-ol, N, N’-Bis-(4-aminophenyl)-1,4-diazacycloheptan, 1, 10-Bis-(2,5-diaminophenyl)-1,4,7,10-tetraoxadecan, 2,4,5,6-Tetraaminopyrimidin, 4-Hydroxy-2,5,6-triaminopyrimidin, 2-Hydroxy-4,5,6-triaminopyrimidin, 2,3-Diamino-6,7-dihydro-1H,5H-pyrazolo-[1,2-a]-pyrazol-1-on, 3-Aminophenol, 5-Amino-2-methylphenol, 3-Amino-2-chlor-6-methylphenol, 2-Hydroxy-4-aminophenoxyethanol, 5-Amino-4-chlor-2-methylphenol, 5-(2-Hydroxyethyl)amino-2-methylphenol, 2.4-Dichlor-3-aminophenol, 2-Aminophenol, 3-Phenylendiamin, 2-(2,4-Diaminophenoxy)ethanol, 1,3-Bis-(2,4-diaminophenoxy)propan, 1-Methoxy-2-amino-4-(2-hydroxyethylamino)benzol, 1,3-Bis-(2,4-diaminophenyl)propan, 2, 6-Bis(2'-hydroxyethylamino)-1 -methylbenzol, 2-({3-[(2-Hydroxyethyl)amino]-4-methoxy-5-methylphenyl}amino)ethanol, 2-({3-[(2-Hydroxyethyl)amino]-2-methoxy-5-methylphenyl}amino)ethanol, 2-({3-[(2-Hydroxyethyl)amino]-4,5-dimethylphenyl}amino)ethanol, 2-[3-Morpholin-4-ylphenyl)amino]ethanol, 3-Amino-4-(2-methoxyethoxy)-5-methylphenylamin, 1-Amino-3-bis-(2-hydroxyethyl)aminobenzol, Resorcin, 2-M ethyl resorcin, 4-Chlorresorcin, 1,2,4-Trihydroxybenzol, 2-Amino-3-hydroxypyridin, 3-Amino-2-methylamino-6-methoxypyridin, 2,6-Dihydroxy-3,4-dimethylpyridin, 3.5-diamino-2,6-dimethoxypyridine, 1-phenyl-3-methylpyrazol-5-one, 1-naphthol, 1,5-dihydroxynaphthalene, 2,7-dihydroxynaphthalene, 1,7-dihydroxynaphthalene, 1,8-dihydroxynaphthalene, 2-(1,3-benzodioxol-5-ylamino)ethanol, (1 -beta-hydroxyethyl-3,4-methylenedioxyaniline), 4-hydroxyindole, 6-hydroxyindole, 7-hydroxyindole, 4-hydroxyindoline, 6-hydroxyindoline and 7-hydroxyindoline.

[0490] In preferred embodiments, the dyes according to the invention have a pH value of about 2 to about 10, preferably about 3 to about 9, more preferably about 3.5 to about 8, particularly preferably about 4 to about 7, in each case measured at 20°C.

[0491] All facts, objects, and embodiments described for the first composition and the second composition according to the invention are also applicable to this subject matter of the invention. Therefore, explicit reference is made here to the disclosure at the relevant point, with the note that this disclosure also applies to the aforementioned dyes, dye kits, and dyeing processes according to the invention. EXAMPLE

[0492] The following example demonstrates that a cosmetic two-component composition, in which component 1 contains the glycosylated dye but no active enzymes, and in which component 2 contains active enzymes, produces a beneficial hair coloring.

[0493] Example 1: Applying a composition for dyeing keratinous fibers

[0494] A first composition ("color gel") according to Table 1 and a second composition ("enzyme gel") according to Table 2 were prepared.

[0495] Table 3: Color source

[0496] Ingredient framework formulation example

[0497] Natural dye preparation 1 0.1-9% 2%

[0498] Microcrystalline cellulose 1-7% 4%

[0499] preservatives

[0500] Caprylyl Glucol 0.3-1% 0.5%

[0501] Na Benzoate 0.1-0.5% 0.3%

[0502] 1,2-Propanediol 5-50% 20%

[0503] Water Ad 100% Ad 100%

[0504] pH 2 4 to 6.5 4.9

[0505]

[0506] Values ​​in wt.% based on the color gel; 1 Plant extract containing ≥ 6% hennoside (glycosylated lawsone) and ≤ 0.2% lawsone; 2 Adjustment using citric acid

[0507] Table 4: Enzyme source

[0508] Ingredient framework formulation example

[0509] β-Glucosidase 0.1-1% (active enzyme protein) 1% (active enzyme protein) Microcrystalline cellulose 1-7% 4%

[0510] preservatives

[0511] Caprylyl Glucol 0.3-1% 0.5%

[0512] Na Benzoate 0-0.5% 0.3%

[0513] 1,2-Propanediol 5-50% 20%

[0514] Water Ad 100% Ad 100%

[0515] pH 1 4 to 9 5.2

[0516]

[0517] Values ​​in wt.% based on the color gel; 1 Adjustment using citric acid and / or NaOH. The first composition and the second composition were mixed in a ratio of 9:1 immediately before application.

[0518] The mixture was either applied directly to the keratinous fibers to be dyed (buffalo belly hair strands, brush body bound, approx. 8 cm free hairs) after mixing or first incubated for 15 minutes at room temperature.

[0519] The exposure time after applying the mixture to the keratinous fibers was 30 minutes, optionally at room temperature or at 37°C (heating hood).

[0520] The mixture was then rinsed out with clear water for approximately 30 to 60 seconds, and the hair was dried using a standard hairdryer (approximately 30 to 60 seconds, distance of hairdryer to hair approximately 10 to 30 cm), and the color result was visually assessed. The results are shown in Table 3.

[0521] Table 5: Results of the hair coloring

[0522] No incubation required after mixing; 30 minutes exposure time at room temperature.

[0523] 15 minutes incubation after mixing, 30 minutes exposure time at room temperature

[0524] 15 minutes incubation after mixing, 30 minutes exposure time at 37°C

[0525]

[0526] All application examples result in a uniform color, which can be further improved by an incubation period after mixing and a warming period. Example 2: Comparison of different enzyme gel compositions

[0527] Various enzyme gels were prepared according to Table 6 and their consistency was examined. This experiment serves to investigate the influence of the respective combination of enzyme and thickener on the consistency, i.e., thickening capacity, of the composition.

[0528] Table 6: Enzyme gel for producing a dye

[0529] E1 V1 V2 V3 V4 V5 V6 V7 ß-glucosidase (tg) 10% 10% 10% 10% Cellulase (tg) 10% 10% 10% 10% Microcrystalline cellulose 4% 4%

[0530] Cellulose Gum 1.5% 1.5%

[0531] Hydroxyethylcellulose 1% 1% 1.5% 1.5% Caprylyl Glucol 1% 1% 1% 1% 1% 1% 1% 1% 1,2-Propanediol 20% 20% 20% 20% 20% 20% 20% Citric Acid 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% 0.05% Water Ad Ad Ad Ad Ad Ad Ad Ad 100% 100% 100% 100% 100% 100%

[0532]

[0533] Values ​​in wt.% based on the enzyme gel

[0534] Table 7: Results

[0535] Consistency of enzyme gel before adding enzyme. Consistency of enzyme gel after adding enzyme E1: viscous.

[0536] V1 viscous, thin viscous

[0537] V2 viscous, thin, with streaks

[0538] V3 viscous, thin, with streaks

[0539] V4 viscous, thin, with streaks

[0540] V5 thick, thin, with streaks

[0541] V6 thick, thin, with streaks

[0542] V7 thick, thin, with streaks

[0543]

[0544] The use of the respective thickeners leads to a thickening of the composition, recognizable by its viscous and thick consistency. In experiments V1 to V7, the addition of the respective enzyme to the thickened composition leads to a liquefaction of the composition; that is, the enzyme hydrolyzes the cellulose-containing thickener.

[0545] Only the combination (E1) of β-glucosidase and microcrystalline cellulose according to the invention results in a viscous and thick composition even after the addition of enzyme, i.e. the combination according to the invention enables the production of an enzyme gel according to the invention, which can be mixed with a color gel according to the invention, as described herein, to produce a dye according to the invention for the non-oxidative coloring of keratin fibers.

Claims

PATENT CLAIMS 1. A cosmetic agent for non-oxidative dyeing of keratinous fibers, especially human hair, comprising two separate compositions, wherein (A) the first composition comprises, in each case based on the total weight of the first composition, a) at least one glycosylated dye, in particular a glycosylated natural dye, preferably in an amount of about 0.1 to about 25 wt.%, more preferably about 0.2 to about 15 wt.%, further preferably about 0.3 to about 2 wt.%, particularly preferably about 0.4 to about 1.4 wt.%, b) at least one first thickener, preferably in an amount of about 0.1 to about 12 wt.%, more preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%, c) optionally at least one preservative, preferably in an amount of about 0.1 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%, and d) at least one organic solvent, preferably in an amount of about 2 to about 50 wt.%, more preferably about 4 to about 50 wt.%, further preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%, wherein the organic solvent consists of C1-4-alkanols and C 2-4 -Polyols, in particular ethanol, isopropanol, n-propanol, ethylene glycol, 1,2-propanediol, glycerol and 1,3-butylene glycol, and mixtures thereof; e) Water, preferably in an amount of more than 45 wt.%, preferably more than 50 wt.%, further preferably more than 60 wt.%, particularly preferably more than 70 wt.% and most particularly preferably up to 75 wt.%; and (B) the second composition comprises, in each case in relation to the total weight of the second composition, a) at least one β-glucosidase (EC 3.2.1.21), preferably in an amount of about 0.001 to about 2.5 wt.%, more preferably about 0.01 to about 2 wt.%, further preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.5 to about 1 wt.%, based on active enzyme protein, b) at least one second thickener, preferably in an amount of about 0.1 to about 12 wt.%, more preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%, c) optionally at least one preservative, preferably in an amount of about 0.1 to about 2 wt.%, more preferably about 0.1 to about 1.5 wt.%, more preferably about 0.3 to about 1 wt.%, d) at least one organic solvent, preferably in an amount of about 2 to about 50 wt.%, more preferably about 4 to about 50 wt.%, further preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%, wherein the organic solvent consists of C1-4- Alkanols and C 2-4 -Polyols, in particular ethanol, isopropanol, n-propanol, ethylene glycol, 1,2-propanediol, glycerol and 1,3-butylene glycol, and mixtures thereof; e) Water, preferably in an amount of more than 40 wt.%, preferably more than 45 wt.%, further preferably more than 50 wt.%, particularly preferably more than 60 wt.% and most particularly preferably up to 65 wt.%. where the first thickener and / or the second thickener is microcrystalline cellulose.

2. Cosmetic product according to claim 1, wherein the first composition has a pH value of about 3 to about 8, preferably about 3.5 to about 7.5, more preferably about 4 to about 7, particularly preferably about 4 to about 6.6, in each case measured at 20°C; and / or The second composition has a pH value of about 2.5 to about 10, preferably about 3 to about 9.5, more preferably about 3.5 to about 9.5, particularly preferably about 4 to about 9, each measured at 20°C.

3. Cosmetic composition according to claim 1 or 2, wherein the first thickener is the same as the second thickener.

4. Cosmetic composition according to claim 1 or 2, wherein the first thickener and the second thickener are different.

5. Cosmetic product according to any one of the preceding claims, wherein if the first thickener is microcrystalline cellulose, the second thickener is selected from (i) a mixture of xanthan gum and scleroglucan gum, (ii) diutan gum, (iii) clay minerals, (iv) a mixture of clay minerals and xanthan gum, and (v) mixtures thereof, or if the second thickener is microcrystalline cellulose, the first thickener is selected from (i) a mixture of xanthan gum and scleroglucan gum, (ii) diutan gum, (iii) clay minerals, (iv) a mixture of clay minerals and xanthan gum, and (v) mixtures thereof.

6. Cosmetic composition according to any of the preceding claims, wherein the first composition and / or the second composition is a water-based gel.

7. Cosmetic composition according to any one of the preceding claims, wherein the at least one glycosylated dye is a glycosylated natural dye obtained from plant parts, powder, or extract, selected from Indigofera, in particular Indigofera tinctoria, Indigo suffruticosa, Indigofera articulata, Indigofera arrecta, Indigofera heterantha "Gerardiana", Indigofera argentea, or Indigofera longiracemosa; Isatis, in particular Isatis tinctoria (woad); Persicaria, in particular Persicaria tinctoria (dyer's knotweed); Wrightia, in particular Wrightia tinctoria; Calanthe, in particular Calanthe veratrifolia', and Baphicacanthus cusia (synonym Strobilanthes cusia, Lawsonia, in particular Lawsonia inermis; and mixtures thereof, especially preferably of Indigofera, in particular Indigofera tinctoria; Lawsonia, in particular Lawsonia inermis; and mixtures thereof.

8. Cosmetic composition according to claim 7, wherein plant parts, powder or extract contain the glycosylated natural pigment in an amount equal to or greater than 6% by weight, preferably equal to or greater than 10% by weight, and / or wherein plant parts, powder or extract contain the non-glycosylated form of the natural pigment in an amount equal to or less than 0.2% by weight, preferably equal to or less than 0.1% by weight.

9. Cosmetic composition according to any of the preceding claims, wherein the first composition is substantially free of enzymes, preferably free of enzymes.

10. Cosmetic composition according to any of the preceding claims, wherein the second composition is substantially free of cellulases (EC 3.2.1.4), preferably free of cellulases (EC 3.2.1.4).

11. Cosmetic composition according to any of the preceding claims, comprising two separate compositions, wherein (A) the first composition comprises, in each case based on the total weight of the first composition, a) at least one glycosylated natural dye in an amount of about 0.1 to about 25 wt.%, preferably about 0.2 to about 15 wt.%, more preferably about 0.3 to about 2 wt.%, particularly preferably about 0.4 to about 1.4 wt.%, wherein the glycosylated natural dye is selected from hennoside and / or indican, b) at least one first thickener in an amount of about 1 to about 12 wt.%, preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%, c) at least one preservative in an amount of about 0.1 to about 2 wt.%, preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.3 to about 1 wt.%, wherein the preservative is selected from 1,2-octanediol, sodium benzoate and mixtures thereof, d) 1,2-propanediol in an amount of about 2 to about 50 wt.%, preferably about 4 to about 50 wt.%, more preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%; e) Water in an amount of more than 40 wt.%, preferably more than 45 wt.%, further preferably more than 50 wt.%, particularly preferably more than 60 wt.% and most particularly preferably up to 65 wt.%; and (B) the second composition comprises, in each case in relation to the total weight of the second composition, a) at least one β-glucosidase (EC 3.2.1.21) in an amount of about 0.001 to about 2.5 wt.%, preferably about 0.01 to about 2 wt.%, further preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.5 to about 1 wt.%, based on active enzyme protein, b) at least one second thickener in an amount of about 1 to about 12 wt.%, preferably about 2 to about 10 wt.%, more preferably about 2.5 to about 9 wt.%, particularly preferably about 3 to about 8 wt.%, c) at least one preservative in an amount of about 0.1 to about 2 wt.%, preferably about 0.1 to about 1.5 wt.%, particularly preferably about 0.3 to about 1 wt.%, wherein the preservative is selected from 1,2-octanediol, sodium benzoate and mixtures thereof, and d) 1,2-propanediol in an amount of about 2 to about 50 wt.%, preferably about 4 to about 50 wt.%, further preferably about 5 to about 30 wt.%, particularly preferably about 10 to about 25 wt.%; e) Water in an amount of more than 40 wt.%, preferably more than 45 wt.%, further preferably more than 50 wt.%, particularly preferably more than 60 wt.% and most particularly preferably up to 70 wt.% where the first thickener and / or the second thickener is microcrystalline cellulose.

12. Method for non-oxidative dyeing of keratinous fibers, in particular human hair, comprising the following process steps in the specified order (a) Providing a first composition, wherein the first composition is according to any one of claims 1 to 11, (b) Providing a second composition, wherein the second composition is according to any one of claims 1 to 11, (c) Mixing the first composition from step (a) with the second composition from step (b) to produce a ready-to-use dye, preferably in a ratio of 15:1 to 3:1, more preferably 12:1 to 5:1, more preferably 10:1 to 7:1, particularly preferably 9:1, (d) optionally incubate the dye from step (c) for 0 to about 45 minutes, preferably about 5 minutes to about 30 minutes, preferably about 10 minutes to about 20 minutes, (e) Applying the dye obtained in step (c) to the keratin fibers, wherein the application of the dye obtained in step (c) to the keratin fibers takes place preferably within about 1 second to about 30 minutes, preferably within about 10 seconds to about 15 minutes after completion of step (c) or (d), (f) Allowing the dye to act on the keratinous fibers, wherein the acting time is preferably about 30 seconds to about 90 minutes, more preferably about 5 minutes to about 60 minutes, more preferably about 15 minutes to about 45 minutes, and / or wherein the acting is preferably carried out at a temperature of about 25°C to about 60°C, more preferably about 30°C to about 50°C, preferably at about 35°C to about 40°C, particularly preferably at about 30°C to about 40°C, and most particularly preferably at about 37°C, (g) Rinsing the keratinous fibers with water, and (h) optional drying of the keratinous fibers.

13. Method according to claim 12, wherein the dye is an agent according to any one of claims 1 to 11.

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