Arrays and methods for spatial epigenomic profiling
DNA arrays with barcoded polynucleotides address the limitations of current spatially-resolved omics technologies by enabling cost-effective and detailed spatial epigenomic profiling in diverse tissues, including FFPE samples, through in-situ transposase loading for histone modification analysis.
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
- Filing Date
- 2025-12-16
- Publication Date
- 2026-06-25
AI Technical Summary
Current spatially-resolved omics technologies face challenges in accessing molecular readouts like chromatin accessibility and epigenetics, particularly in formalin-fixed paraffin-embedded tissues, and are costly, limiting their applicability and integration in multi-omic views.
Development of DNA arrays with barcoded polynucleotides harboring a mosaic sequence, allowing in-situ loading of transposase for spatial epigenomic profiling, enabling cost-effective and large-scale analysis of histone modifications in various tissue types, including FFPE samples.
The DNA arrays provide detailed molecular views of chromatin regulation by revealing active, repressive, or bivalent chromatin states across tissues, offering a cost-effective and efficient method for spatial epigenomics in fresh-frozen and FFPE tissues.
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