Perfusion module for a bioreactor, and system comprising a perfusion module and a bioreactor

Abstract

The invention relates to a perfusion module (100, 400, 600, 900) for a bioreactor, comprising a main body (102, 402, 602, 902) having a fluid passage (110, 410, 610, 910) and a recess (112, 412, 612, 912) inclined toward said fluid passage (110, 410, 610, 910), a flow-permeable flow guidance element (104, 404, 604, 904), a filter element (106, 406, 606, 906), and at least one fastening element (108, 408, 608, 908), wherein the at least one fastening element (108, 408, 608, 908) is designed to fasten the filter element (106, 406, 606, 906) to the flow guidance element (104, 404, 604, 904), wherein, when the perfusion module (100, 400, 600, 900) is in an assembled condition, the flow guidance element (104, 404, 604, 904) is located between the main body (102, 402, 602, 902) and the filter element (106, 406, 606, 906), and the recess (112, 412, 612, 912) of the main body (102, 402, 602, 902) and the flow guidance element (104, 404, 604, 904) together form a cavity (200, 500, 700, 1000) that is connected to the fluid passage (110, 410, 610, 910) and to the flow guidance element (104, 404, 604, 904) such as to be flow-permeable.

Description

[0001] December 17, 2025

[0002] Perfusion module for a bioreactor and system with a perfusion module and a bioreactor

[0003] The present invention relates to a perfusion module for a bioreactor and to a system comprising such a perfusion module and a bioreactor.

[0004] In chemistry or biology, for example for microbiological investigations or cell cultures, it is common to introduce or remove a reagent or medium from a vessel. This is the case, for instance, when a nutrient medium is added for a cell culture. A challenge here is to keep the objects of investigation, such as biological material (e.g., human or animal cells, bacteria, archaea, fungi, plants, enzyme reactions), within the vessel and to remove only a fluid (e.g., a nutrient medium), waste products, or similar substances without damaging the remaining biological material, impairing its function, or negatively affecting its development (growth or developmental biology).

[0005] It is known from the prior art to fix biological material onto a solid support material while a nourishing fluid is exchanged for the biological material or flows past the fixed biological material. A disadvantage of this method is that the biological material is fixed, which is undesirable in certain investigations or cultivations and complicates further processing. Another known approach involves using a filter or membrane element to retain the biological material when used medium, waste products of the biological material, or other fluids are removed. However, a common problem arises here: due to the pressure differential or suction used to remove the medium, the biological material adheres to the filter or membrane element and may clog pores or openings of the filter or membrane element.Due to the biological properties of the filtrate, organisms can actively adhere to and within the filter medium, leading to so-called "bio-fouling".

[0006] Against this background, the present invention aims to overcome the disadvantages of the prior art and, in particular, to provide improved means for introducing or removing a fluid into or from a biological system.

[0007] The aforementioned problem is solved by a perfusion module for a bioreactor, comprising a base body having a fluid passage and a recess converging towards the fluid passage, a flow-permeable flow guide element, a filter element and at least one fastening element, wherein the at least one fastening element is designed to fasten the filter element to the flow guide element, wherein, in an assembled state of the perfusion module, the flow guide element is arranged between the base body and the filter element and the recess of the base body and the flow guide element together form a cavity permeable to flow between the fluid passage and the flow guide element.

[0008] The aforementioned task is also solved by a system with a perfusion module as disclosed and with a bioreactor having a fluid container, in that the perfusion module forms the bottom of the fluid container.

[0009] The interaction of the base body, the flow guide element, and the filter element, and their specific design, can achieve improved flow when introducing or removing a fluid into or out of a biological system. In particular, the solution as disclosed allows, on the one hand, a

[0010] GO 241130WO December 17, 2025, a more homogeneous flow field can be achieved at the filter element.

[0011] On the other hand, a relatively large filter area is provided at the filter element or at the bottom of the reactor chamber. Both aspects together create the synergistic effect of a relatively low filtration rate per unit area, which slows down clogging or blockage of the filter element. Furthermore, biological material, especially cells, is treated more gently due to the lower flow forces at the filter element. At the same time, the filter area in the reactor can be almost continuously flushed during mixing, which further reduces clogging. This also allows for better control of fouling.

[0012] The suitability of the perfusion module as a base for a fluid container allows the system to expand its functionality for bioreactors, especially for shear-sensitive organisms such as stem or immune cells.

[0013] According to the disclosure, the flow-guiding element is arranged between the base body and the filter element. Furthermore, the recess of the base body and the flow-guiding element together form a cavity permeable to the fluid passage and the flow-guiding element. The recess of the base body slopes towards the fluid passage. Thus, the cavity is designed to guide a fluid entering the cavity through the flow-guiding element to the fluid passage in a flow-optimized manner when a first main flow direction is present. Such a flow direction can occur, for example, in the application of filtration, where suction is applied at the fluid passage. This suction can be generated, for example, by a pump connected to the fluid passage or by hydrostatics.When flowing in the first main direction, a fluid can be distributed across the surface of the filter element and drawn towards the fluid passage by a narrowing current within the cavity. Thanks to the suction effect distributed across the entire surface of the filter element, otherwise flow-related accumulation or deposits of biological material are prevented.

[0014] GO 241130WO December 17, 2025: Increased currents in the bioreactor are reduced. In other words, the high filter surface area to reactor volume ratio allows biological material to be filtered with little suction or pressure differential, which makes potentially irreversible clogging of the filter medium more difficult. Deposits on the filter surface can then be backwashed essentially evenly.

[0015] The design of the cavity as disclosed also makes it possible to distribute a fluid entering through the fluid passage within the cavity in a second main flow direction, or to broaden the incoming flow within the cavity and guide it in a broadened flow across the surface of the flow-guiding element. The uniform inflow of the liquid through a large cross-section reduces strong or rapid flow movements at the filter surface. This enables, in particular, a uniform and controllable backwashing or slurry formation at the filter element, which is advantageous for shear-sensitive cells.

[0016] Designing the perfusion module as the base for the fluid reservoir of a bioreactor enables media exchange during the bioreactor's operation, or so-called "in-situ" media exchange. This allows nutrients to be introduced and metabolic waste products to be flushed out during a process similar to cell culture. This results in a higher concentration of biological material in the system and / or a higher process yield in so-called "biology productions," i.e., in cases where cells are not the product but rather produce and expel the desired product. Furthermore, a rapid concentration of the components retained in the bioreactor or by the filter element can be achieved, thereby facilitating the harvesting of biological material and intensifying downstream processing. Overall, the perfusion model described above enables process intensification.

[0017] GO 241130WO December 17, 2025 Furthermore, the suitability of the perfusion module as the base of the fluid tank reduces the need for separate components. This also allows for a space-saving system design and reduces the likelihood of contamination compared to external perfusion systems.

[0018] For the purposes of this disclosure, bioreactors can be fluid containers in embodiments commonly used in chemistry and bioprocess engineering, whether intended for multiple or single use. A non-exhaustive list of examples includes: stirred tank reactors, bubble column reactors, fixed-bed reactors, fluidized-bed reactors, membrane bioreactors, photobioreactors, reactors for tissue engineering, roller bottles, flasks, and wave bags.

[0019] The bioreactor preferably comprises a fluid vessel with a wall comprising a bottom and a side, and a lid compatible with the fluid vessel. The fluid vessel and the lid can form a housing that includes a cavity for holding at least one fluid. This cavity can be called the reactor chamber and can be of any size. The bioreactor can thus have a working volume in the range of 0 to 2000 L, particularly in one of the following ranges: 0.1 to 0.3 L, 0.3 to 2 L, 2 to 10 L, 10 to 50 L, 50 to 200 L, or 200 to 2000 L. Particularly preferred values ​​for the working volume of the bioreactor are: 0.3 L, 2 L, 10 L, 50 L, 200 L, and 2000 L. Working volumes up to approximately 2 L are particularly suitable for research and development purposes for therapeutic agents. Working volumes in the range of approximately 2 to approximately 10 L are particularly suitable for chemistry studies as preparation for production processes.Working volumes from approximately 10 L are particularly suitable for production processes.

[0020] Optionally, the bioreactor can have a gassing and stirring unit located within the reactor chamber. This gassing and stirring unit can include at least one membrane element and can be used for fluid transfer, for example.

[0021] GO 241130WO 17 December 2025 is designed for gas transfer as well as for power transmission to drive the aeration and stirring unit. Alternatively or additionally, the bioreactor may have a stirrer, for example a so-called "Rushton impeller" or a skewed blade stirrer.

[0022] The perfusion module is preferably designed to serve as the base of the fluid vessel of a bioreactor. For this purpose, the perfusion module can have a connection area for a fluid-tight seal with the wall of the fluid vessel. A sealing element, such as a sealing ring or an adhesive compound, can be provided at this connection area. The connection area can be located on the base body, the filter element, or the mounting element. Alternatively, at least two elements consisting of the base body, the filter element, or the mounting element can form the connection area. Alternatively, the wall of the fluid vessel can have a section designed as a mounting element.

[0023] The fluid passage can be designed for flow in a first main flow direction and in a second main flow direction opposite to the first. This allows the perfusion module to be used both for introducing a fluid into the reactor chamber of the bioreactor, for example for backflushing, and for removing a fluid from the reactor chamber, for example for filtration.

[0024] The fluid passage can be designed to introduce a fluid into or out of the cavity. The fluid may be subject to suction or a pressure differential created by a pump.

[0025] According to the disclosure, the recess of the base body tapers towards the fluid passage. In this context, the recess can have a funnel-shaped profile when viewed in cross-section, with the funnel-shaped profile tapering towards the fluid passage.

[0026] GO 241130WO December 17, 2025 The at least one fastening element is designed, as disclosed, to attach the filter element to the flow guide element. For this purpose, the at least one fastening element may have a connecting section that can be connected to the base body and a receiving area for receiving the flow guide element and the filter element. Thus, the at least one fastening element is designed to attach the flow guide element and the filter element to the base body. Alternatively or additionally, the base body may have a counter-fastening element that can be connected to the at least one fastening element.

[0027] Examples of a fastening element include a thread or clamping mechanism, an element for receiving an adhesive, an adhesive, a welded joint, without these being limiting.

[0028] Multiple fastening elements can be provided, or the single fastening element can be multi-part. The fastening elements can be distributed across the filter element, either section by section or at specific points, to attach the filter element to the flow guide element. A large filter element can be attached using multiple fastening elements or the multi-part fastening element. In this way, the perfusion module can be adapted to large bioreactors.

[0029] Alternatively or additionally, at least one fastening element can be connected to the flow guide element. The flow guide element can, in turn, be connected to the base body. In this way, the filter element can be attached to the flow guide element via the fastening element, and the flow guide element can then be attached to the base body.

[0030] Preferably, the fastening element is designed to clamp the filter element to or above the flow guide element.

[0031] G0241130WO December 17, 2025 The following describes various embodiments of the perfusion module and the system, with each embodiment applying independently to the perfusion module and the system. Furthermore, the individual embodiments can be combined with one another as desired.

[0032] In one embodiment of the perfusion module, the filter element has a first separation efficiency, the flow guide element has a second separation efficiency, and the first separation efficiency is greater than the second separation efficiency.

[0033] When suction or a pressure differential is applied to the fluid passage of the perfusion module, biological material can be gently retained in the reactor chamber of a connected bioreactor using the filter element, which has the first sieve efficiency. Additionally, fluid drawn in by the suction can be fluidized into the cavity by means of the flow guide element, which has the second sieve efficiency, and distributed across its surface. In a specific example, the perfusion module can be used to retain cells, cell aggregates, or microcarriers via the filter element, while simultaneously directing a medium for cell cultivation out of the reactor chamber via the flow guide element, either across the bottom of the bioreactor or through the filter element of the perfusion module.

[0034] The separation efficiency (η) describes the effectiveness of a separation process. Specifically, the separation efficiency corresponds to the ratio of the amount or concentration of the substance to be separated (e.g., biological material) that is separated by the filter element or flow guide element to the concentration of the medium.

[0035] GO 241130WO 17 December 2025 where the substance to be separated is separated by the filter element or by the flow guide element.

[0036] The permeability of the filter element and the flow guide element can be enhanced by at least one element from the following list, which is not exhaustive: pore size, porosity, tortuosity, pore distribution, opening size (e.g., opening diameter), distribution of penetrating openings, pore density or density of penetrating openings, and permeability. In a specific example, the filter element may have pores with an average pore size, the flow guide element may have penetrating openings with an average opening size, and the pore size may be equal to or smaller than the opening size.

[0037] The filter element can have pores with an average pore size in the range of 0.2 to 200 pm, although this range is not limiting.

[0038] Alternatively or additionally, the filter element can have penetrating openings, the size of which is smaller than the size of the components of a biological material or than the size of carriers for the biological material that are to be retained in the reactor space. This allows the biological material to be filtered from a fluid; the penetrating openings can, for example, have an average size in the range of 0.2 to 200 pm.

[0039] Depending on the selected initial permeability, pore size, or opening size of the filter element, the perfusion module can be used for various applications. For example, depending on the pore size or the size of the penetrating openings, the perfusion module can be used for carrier separation or for separating cells, cell aggregates, and microcarriers. Further application examples include the separation of dissolved components of the medium, such as in dialysis, of large proteins like antibodies and enzymes, of small proteins, and of individual salts.

[0040] GO 241130WO 17 December 2025, for example, urea. The separation can occur against the background of charge differences.

[0041] The filter element can, for example, have a membrane of one of the following types: dense membrane for diffusive entry, porous membrane, woven membrane, spun membrane. The membrane can be made of at least one of the following materials: plastic, ceramic, metal, or natural materials such as cellulose.

[0042] In one embodiment of the perfusion module, the flow guidance element is provided to have pores and / or penetrating openings.

[0043] This allows for a flow distribution across the surface of the flow guidance element.

[0044] The penetrating openings of the flow-guiding element preferably each have a principal direction of extension, with the respective principal directions of extension converging towards the fluid passage of the base body. Alternatively, the respective principal directions of extension of the penetrating openings of the flow-guiding element can be parallel to each other. By aligning the principal directions of extension of the openings, the flow can, for example, be directed towards the fluid passage or distributed over the surface of one side of the flow-guiding element.

[0045] In one embodiment of the perfusion module, the flow guide element has a central region that is free of pores and / or penetrating openings. This improves fluid flow and, in particular, prevents increased flow into the central region of the flow guide element.

[0046] GO 241130WO 17 December 2025 In one embodiment of the perfusion module, it is provided that the pores and / or the penetrating openings are distributed essentially homogeneously over an area of ​​the flow guidance element.

[0047] This allows for a uniform distribution of the flow of a fluid passing through the flow guide element. A relatively small cross-section of the pores or openings in the flow guide element contributes to creating flow resistance. This flow resistance causes any negative or positive pressure below the flow guide element to be distributed evenly across the entire surface of the flow guide element within the cavity. In this way, the flow through the pores or openings is as uniform as possible.

[0048] In one embodiment of the perfusion module, the pores and / or the penetrating openings are distributed over an area of ​​the flow guidance element according to a mathematical function, wherein the mathematical function is based on an angular function based on a multiple of the golden angle and on a radial distribution function.

[0049] The radial distribution function, combined with the angle function based on the golden angle, creates a non-periodic yet uniform pore distribution across the surface of the flow-guiding element. This novel approach to pore arrangement improves the flow distribution. Furthermore, the porosity of the flow-guiding element can be maintained across different system scales, thus enabling comparability between systems of varying sizes.

[0050] Furthermore, compared to, for example, a grid-like distribution of pores and / or penetrating openings, truncated pores and / or penetrating openings at the edge of the flow-guiding element can be avoided. In addition, this allows for complete rotational symmetry of the pore distribution.

[0051] GO 241130WO December 17, 2025 In a particular embodiment, the mathematical function can be based on phyllotaxis. This distribution method uses the golden angle g to determine the angular position yi of a pore i. The angular position y^ can be expressed as follows:

[0052] γᵢ = g · i

[0053] It can be assumed that the golden angle is approximately 137.5°.

[0054] To position the pores or penetrating openings at different radii, a radial distribution function can be used. For this purpose, a radius can be assigned to each pore. The fraction of the circular area spanned by a given pore radius, relative to the flow-guiding element surface, can correspond to the fraction of the respective pore index relative to the total number of pores. This fraction can be expressed as follows:

[0055] Af nr 2 i

[0056] A str . ~ n r st r 2 ~ n

[0057] where A[ corresponds to the area of ​​the pore with index i; where

[0058]

[0059] corresponds to the area of ​​the flow guiding element; where r i the radial position of the pore with the index i on the surface of the flow-guiding element; where r str- which corresponds to the radius of the flow-guiding element; and where n corresponds to the number of pores. From this, the following expression can be derived:

[0060] Ji

[0061] ~ rt ' ^ir

[0062]

[0063] In practice, with l / nThe pores are placed at the boundary of their radial ring segment, meaning that the pores are aligned with the edges of these concentric regions. To achieve a more balanced distribution, where the individual pores are located more towards the center of their respective radial segment rather than at its boundary, the index can be shifted by half a step. Overall, the radial distribution function can be expressed as follows:

[0064] G0241130WO December 17, 2025 i - 0.5

[0065] ■ r S tr.

[0066]

[0067] In combination with the trigonometric function based on a multiple of the golden angle, this radial distribution function can achieve a non-periodic yet uniform pore distribution across the flow guide element, inspired by natural phyllotaxis. In particular, this allows for an approximately equal angular area distribution of the pores across the surface of the flow guide element.

[0068] In one embodiment of the perfusion module, it is provided that the flow guiding element is essentially rotationally symmetrical relative to an axis of rotation and has a porosity that decreases in the direction of the axis of rotation, and / or that the flow guiding element is essentially rotationally symmetrical relative to an axis of rotation and the penetrating openings have a size that decreases in the direction of the axis of rotation, and / or that the flow guiding element is essentially rotationally symmetrical relative to an axis of rotation and the penetrating openings are arranged with a distance to each other that increases in the direction of the axis of rotation.

[0069] Such distributions of pores and / or penetrating openings in the flow guide element allow for a gradual flow distribution, for example, with a gradually increasing or decreasing flow velocity when viewed in a cross-sectional view of the flow guide element. For this purpose, the flow guide element can exhibit a gradient with respect to the size of the pores or penetrating openings. Alternatively or additionally, the flow guide element can exhibit a gradient with respect to the spacing of the pores or penetrating openings. The gradient can be particularly evident in a radial cross-sectional view of the flow guide element.

[0070] GO 241130WO 17 December 2025 A rotational symmetry within the meaning of the present disclosure can be described with an axis of rotation and with a rotation index, wherein the rotation index denotes the number of repetitions of a pattern core element around the axis of rotation. The rotation index can be equal to or greater than one.

[0071] In one embodiment of the perfusion module, it is provided that, viewed in a radial cross-sectional view, the flow guide element has a basic profile, wherein the basic profile is essentially flat, or that, viewed in a radial cross-sectional view, the flow guide element has a basic profile, wherein the basic profile is curved, or that, viewed in a radial cross-sectional view, the flow guide element has a first basic profile and a second basic profile, wherein the first basic profile is essentially flat and the second basic profile is curved.

[0072] The curvature allows the filter element to be positioned and held in such a way that the flow at the perfusion module or at the filter element in the reactor space can be adapted to a stirring flow in the reactor space.

[0073] The filter element can be attached to the flow guide element by at least one fastening element in such a way that the filter element is tensioned according to the curvature of the flow guide element. One example of this is clamping the edge of the filter element between the fastening element and the flow guide element. In another example, the fastening element is designed with a mesh-like area which, when the perfusion module is assembled, presses the filter element against the flow guide element.

[0074] Alternatively or additionally, the filter element can be connected to the flow guide element by gluing, welding or electrostatic adhesion, whereby

[0075] GO 241130WO December 17, 2025 The filter element essentially assumes the shape of the flow guide element. In this way, the filter element can assume a convex shape, even in the absence of a pressure gradient. The filter element can be locally bonded or welded to the flow guide element, for example, with individual spacers of the flow guide element.

[0076] Alternatively or additionally, the filter element can assume a curved position corresponding to the curvature of the flow guide element due to an applied suction or pressure difference between the reactor space and the cavity.

[0077] A basic profile can be understood as a profile that characterizes a main extent surface of one side of the flow guidance element. In a specific example, if the flow guidance element has spacers, for example in the form of bumps or protrusions, then the profile of the surface of the flow guidance element without the spacers can be considered the basic profile.

[0078] In particular, one side of the flow guide element can have the first basic profile, while a second side, facing opposite the first, has the second basic profile. With sides having different basic profiles, the flow in the cavity of the perfusion module and the flow at the surface of the filter element in the reactor chamber can be influenced.

[0079] The flow guide element can be designed such that its curvature is directed towards the fluid outlet of the base body. This creates a local low point on the surface of the filter element stretched over the flow guide element. This allows the fluid to be removed from the reactor chamber as completely as possible.

[0080] Alternatively, the flow guidance element can be designed such that the curvature is in the direction opposite to the fluid outlet of the base body.

[0081] GO 241130WO, dated December 17, 2025, allows the flow on the surface of the filter element to be adapted to the flow conditions in the reactor chamber. For example, the curvature can be tailored to the arrangement of a gassing and stirring unit to achieve the most homogeneous flow possible in the reactor chamber.

[0082] Preferably, in an assembled state of the perfusion module, the filter element is clamped onto the flow guide element by the fastening element.

[0083] In one embodiment of the perfusion module, the flow guidance element, viewed in a radial cross-sectional view, has a base profile, wherein the base profile is multiply curved or corrugated. This allows the flow pattern in the reactor chamber to be spatially controlled by the perfusion module.

[0084] In one embodiment of the perfusion module, it is provided that the flow guide element has at least one spacer, and that the at least one spacer is designed to support the filter element held on the flow guide element by the at least one fastening element.

[0085] The spacers allow the filter element to be held at a distance from the base surface of the flow guide element. This creates a gap between the filter element and the base surface of the flow guide element. This gap, in addition to the cavity formed by the recess of the base body and the flow guide element, can contribute to the distribution of the fluid flow.

[0086] In the case where the sum of the cross-sectional areas of the penetrating openings of the flow guiding element is significantly smaller than the total area

[0087] GO 241130WO 17, December 2025 of the filter, for example in a ratio of 20 to 80%, a significantly larger area of ​​the filter can be flowed through by means of the spacers or the space between the filter element and the base of the flow guide element.

[0088] The spacers, like the pores and / or penetrating openings, can be distributed across the surface of the flow guide element according to a mathematical function. This mathematical function comprises an angular function based on a multiple of the golden angle and a radial distribution function. The angular positioning function for the spacers can specify an offset of half the golden angle. This ensures that the spacers are not located in the same position as the pores.

[0089] In particular, if the flow guidance element is designed with a curved base profile, the filter element can be supported in a correspondingly curved position and simultaneously spaced away from the base surface of the flow guidance element.

[0090] The inclusion of spacers enables a three-dimensional lateral structuring of the flow guide element and a corresponding three-dimensional positioning of the filter element. This allows the filter element to be adapted to the flow conditions in the reactor, resulting in rapid and gentle resuspension of sedimented biological material such as microorganisms, organoids, cells, or other deposits (fouling).

[0091] Fouling control can be achieved in the reactor room.

[0092] In one embodiment of the perfusion module, viewed in a radial cross-sectional view, at least one spacer has a curved or corrugated profile. This allows the filter element to be held in a curved position, thus providing the same advantages as with a

[0093] GO 241130WO December 17, 2025 can be achieved with a curved or corrugated base profile of the flow guidance element.

[0094] In one embodiment of the perfusion module, it is provided that at least two spacers are provided, wherein the at least two spacers are essentially the same height relative to a basic profile of the flow guiding element, or that at least two spacers are provided, wherein the at least two spacers are of different heights relative to a basic profile of the flow guiding element.

[0095] When the flow guide element has a flat base profile, the different heights of the spacers allow the filter element to be supported in a curved position. Conversely, when the flow guide element has a curved base profile, the spacers, which are essentially the same height, allow the filter element to be supported in a curved position corresponding to the curvature of the base profile.

[0096] In one embodiment of the perfusion module, it is provided that the at least one spacer or the at least two spacers form a rotationally symmetrical pattern.

[0097] This allows for an optimized distribution of the flow across the surface of the filter element. Additionally, it reduces the risk of clogging or blockage of pores or penetrating openings in the filter element. The rotational symmetry, or a rotation parameter that characterizes the rotationally symmetrical pattern, can be adapted to a stirred flow intended for the reactor chamber of a bioreactor connected to the perfusion module. Such a stirred flow can be generated, for example, by a gassing and stirring unit.

[0098] GO 241130WO December 17, 2025 In one embodiment of the perfusion module, it is provided that, in an assembled state of the perfusion module, the filter element and the at least one fastening element together form a surface with a medium collection area.

[0099] The medium collection area facilitates the emptying of the bioreactor or its fluid container. Furthermore, it helps to collect components separated during cultivation by the rotation of the aeration and stirring unit.

[0100] The medium collection area can be formed as a local low point in the direction of the gravitational force. This allows collection to be achieved through centripetal acceleration and / or due to the gravitational force.

[0101] Alternatively or additionally, the medium collection area can be designed as the section of the fluid container that is furthest from the rotational axis of the aeration and stirring unit. This allows for the fluidic collection of components separated during the rotation of the aeration and stirring unit along its axis.

[0102] Examples of the design of the medium collection area are: circumferential channel in the bottom of the fluid container, conical area in the bottom of the fluid container, valleys in the bottom of the fluid container, although this list is not exhaustive.

[0103] In one embodiment of the perfusion module, it is provided that, in an assembled state of the perfusion module and viewed in a radial cross-sectional view, the filter element and the at least one fastening element together have a curved profile with a bulge formed towards the center of the filter element, and that the flanks of the bulge form a medium collection channel around the filter element.

[0104] GO 241130WO December 17, 2025 The curvature allows for the optimization of flow patterns in the reactor space, especially in combination with a rotational movement of the gassing and stirring unit.

[0105] The curvature can be oriented towards the reactor chamber. In this way, the medium collection area can, for example, be designed as a circumferential trough.

[0106] Alternatively, the bulge can be directed in the opposite direction to the reactor chamber. This allows a medium collection area to be formed as a local low point.

[0107] The curvature of the bottom sections can correspond to a curve with a radius of curvature. Additionally, the bottom section of the fluid container can have a diameter corresponding to the inner diameter of the fluid container. In one embodiment of the bioreactor, the curvature is designed such that the radius of curvature is equal to or greater than half the diameter of the bottom section, in particular that the radius of curvature is equal to or greater than the diameter of the bottom section, preferably that the radius of curvature is in the range of the diameter of the bottom section up to five times the diameter of the bottom section.

[0108] The specified curvature allows the medium to flow into the medium collection area while minimizing negative impacts on the flow. If the ratio of the curvature radius to the diameter of the base area is greater than specified, the base is too flat to form a medium collection area. If the ratio is smaller than specified, the increased curvature can negatively affect the flow pattern in the reactor chamber and the flow into the membrane module.

[0109] GO 241130WO December 17, 2025 In one embodiment of the perfusion module, it is provided that the recess of the base body has an outer circumference and the fluid passage is substantially centered relative to the outer circumference.

[0110] A significant advantage of this is that the perfusion module can be designed with rotational symmetry, allowing the pressure and flow in the reactor space to develop more uniformly.

[0111] In one embodiment of the perfusion module, the recess has an outer circumference and the fluid passage is essentially decentered relative to the outer circumference.

[0112] A decentralized arrangement is particularly suitable when designing the perfusion module for a bioreactor with a relatively large reactor space, for example approximately 2 L or approximately 10 L, in order to improve the accessibility of the fluid passage.

[0113] In one embodiment of the perfusion module, it is provided that at least one support element is provided in the recess of the base body, and that the at least one support element is designed to support the flow guidance element in an assembled state of the perfusion module and under negative or positive pressure in the cavity.

[0114] This increases the mechanical stability of the perfusion module and thus also the reliability in use and the overall longevity of the perfusion module.

[0115] The at least one support element can be provided on the flow guide element and / or on the base body. Alternatively, the support element can be designed as a separate component and for arrangement within the cavity.

[0116] GO 241130WO 17 December 2025 In one embodiment of the perfusion module, it is provided that at least one segmentation element is provided in the recess of the base body for segmentation, in particular for the essentially isovolumetric segmentation of a fluid flow over the volume of the cavity.

[0117] With the aid of at least one segmentation element, a fluid flow segmentation of a fluid flow passing through the cavity can be achieved, thus reducing local blockage of the pores or penetrating openings of the filter element or flow guide element due to heterologous pressure differences.

[0118] Isovolumetric segmentation of the cavity can be understood as a division of the cavity into sub-volumes of equal size.

[0119] Preferably, the at least one support element and the at least one segmentation element are formed by protrusions in the depression of the base body. In particular, protrusions in the depression of the base body can assume the role of both the support element and the segmentation element.

[0120] In one embodiment of the perfusion module, a bearing element is provided for receiving an axis.

[0121] As the size or volume of a bioreactor increases, the mechanical requirements can change. Stirring the fluid can be advantageous for certain reactions, which is why a movable stirring element can be installed in the reactor chamber. With a relatively large reactor chamber and a correspondingly large volume of fluid, for example, one or more liters, moving the stirrer can become difficult. In such cases, mounting the stirrer through the bottom of the bioreactor can be particularly helpful. The disclosed embodiment allows for such a mounting.

[0122] GO 241130WO December 17, 2025 Storage must also be ensured when inserting a perfusion module as disclosed.

[0123] The bearing element can have a material composition that exhibits a low friction ratio with the material of the axle to be mounted.

[0124] In addition, the bearing element can have a shape that is adapted to the shape of the axle to be accommodated.

[0125] In one embodiment of the system, the bioreactor is provided to have a stirrer arranged in the fluid container with an axis of movement, and the axis of movement is received by the bearing element of the mounting element of the perfusion module.

[0126] This allows the system to be designed to be particularly stable, mechanically precise, and safe. In particular, the system can be scaled for larger bioreactor volumes. This enables adequate stirring or mixing of the fluid or biological system within the reactor chamber, as well as the introduction or removal of components from the fluid into or out of the biological system.

[0127] In one embodiment of the perfusion module, the bearing element is formed on the fastening element. Alternatively, the bearing element can be formed on the base body.

[0128] Integrating the bearing element into the mounting element is particularly suitable when the mounting element is part of the reactor chamber wall. This allows the agitator to be stably held by the bearing element.

[0129] An example of how the bearing element can be designed on the fastening element is that the fastening element is designed with an annular section and a platform. The annular section can be used for fastening.

[0130] G0241130WO December 17, 2025 of the edge of the filter element on the base body. The platform can be arranged concentrically relative to the annular section and include the bearing element. The annular section and the platform can be connected by webs. The webs can be designed and / or arranged according to the support element of the base body. Such a design and / or arrangement of the webs enables the lowest possible flow disturbance.

[0131] Designing the base body allows for a simplified design of the fastening element.

[0132] In one embodiment of the perfusion module, the fastening element comprises a first fastening part and a second fastening part, wherein the first fastening part is designed to fasten the filter element to the flow guide element and / or to the base body, and wherein the second fastening part is designed to fasten the filter element to the flow guide element and / or to the base body and comprises the bearing element.

[0133] The two-part mounting element allows the filter element to be held to the base body on the one hand, and on the other hand, an axle, in particular the axle of a bioreactor stirrer, to be accommodated. In a specific example, the first and second mounting parts can be designed as connecting pieces. Alternatively or additionally, the first and second mounting parts can be connected to each other by a positive-locking connection. Alternatively or additionally, the first mounting part can be designed to attach the filter element to an edge region of the base body. The second mounting part can be designed to attach the filter element to a central region of the base body. In this example, the second mounting part enables a centered position of the bearing element on the base body.

[0134] GO 241130WO December 17, 2025 Basic body. This design is particularly advantageous when the filter element is ring-shaped.

[0135] In one embodiment of the perfusion module, the fastening element may comprise a first fastening part and a second fastening part, wherein the first fastening part is designed to fasten the filter element to the flow guide element and / or to the base body when a fluid flows through the filter element in a first direction, and wherein the second fastening part is designed to fasten the filter element to the flow guide element and / or to the base body when a fluid flows through the filter element in a second direction.

[0136] In this way, the mounting of the filter element can be designed to be dependent on the flow direction. In a specific example, the first mounting element can be designed to attach the filter element to the flow guide element and / or the base body when the flow is directed towards the reactor chamber. The second mounting element can be designed to attach the filter element to the flow guide element and / or the base body when the flow is directed away from the reactor chamber.

[0137] In one embodiment of the perfusion module, it is provided that the recess of the base body has an outer circumference, and that, in an assembled state of the perfusion module, the bearing element is essentially centered relative to the outer circumference of the recess.

[0138] A centered positioning of the bearing element allows for the centered mounting of an agitator shaft within the bioreactor chamber, particularly when the perfusion module forms the base of the bioreactor. This ensures a uniform flow within the reactor chamber. This is especially advantageous when combined with a centered fluid outlet on the base body.

[0139] GO 241130WO 17 December 2025 In one embodiment of the system, it is provided that the fluid container comprises a wall with a bottom area and a wall area, that the wall area has the at least one fastening element of the perfusion module, and that the bottom area is formed by the filter element, the flow guide element and the base body of the perfusion module.

[0140] In this embodiment, the fastening element is designed as part or section of the wall area. In this way, a separate design of the fastening element from the wall area can be dispensed with.

[0141] In one embodiment of the system, the fluid container comprises a one-piece wall with a bottom section and a wall section, the bottom section having the fluid passage and the recess and forming the base body of the perfusion module. Alternatively, the fluid container comprises a one-piece wall with a bottom section and a wall section, the bottom section forming the flow-guiding element of the perfusion module. Alternatively, the fluid container comprises a one-piece wall with a bottom section and a wall section, the bottom section forming the filter element of the perfusion module.

[0142] In all three alternatives, one component of the perfusion module is formed by the bottom section of the fluid container. This reduces the number of separate components of the perfusion module and thus also the effort required to connect the individual components.

[0143] In another embodiment of the system, the fastening element is connected to the base body by means of an adhesive bond. This allows the perfusion module to be manufactured simply and cost-effectively.

[0144] GO 241130WO December 17, 2025 In a further embodiment of the system, a check valve is installed upstream of the fluid passage in the base body. This allows the flow to be blocked or allowed to pass depending on its direction. Multiple check valves can be provided, enabling targeted and directed fluid supply and / or discharge. For example, several sources or receiving containers can be connected to the reactor chamber via the perfusion module, allowing multiple fluids to be supplied to or discharged from the reactor chamber.

[0145] In another embodiment of the system, the perfusion module is detachably connected to the bioreactor's fluid reservoir. This allows the perfusion module to be replaced and, if necessary, easily cleaned. The possibility of replacing the bottom of the fluid reservoir, particularly with a perfusion module as disclosed, increases the bioreactor's range of functionalities. Furthermore, the detachably removable perfusion module is suitable for single use, thus eliminating the need for complex cleaning or sterilization procedures.

[0146] In another embodiment of the system, the perfusion module is at least partially manufactured using additive manufacturing or forming processes. Other possible manufacturing methods include machining and photopolymerization. This allows for complex perfusion module designs, for example, with flow-optimized support elements or segmentation elements.

[0147] Further features and advantages of the perfusion module and the system will become apparent from the following description of exemplary embodiments, with reference to the attached drawing.

[0148] The drawing shows

[0149] GO 241130WO 17 December 2025 Fig. 1 a first embodiment of a perfusion module in a perspective exploded view;

[0150] Fig. 2 shows the perfusion module from Fig. 1 in a cross-sectional view;

[0151] Fig. 3 shows a first embodiment of a system with a bioreactor and with the perfusion module from Fig. 1;

[0152] Fig. 4 shows a second embodiment of a perfusion module in a perspective exploded view;

[0153] Fig. 5 shows the perfusion module from Fig. 4 in a cross-sectional view;

[0154] Fig. 6 shows a third embodiment of a perfusion module in a perspective exploded view;

[0155] Fig. 7 shows the perfusion module from Fig. 6 in a cross-sectional view;

[0156] Fig. 8 shows a curved profile of the perfusion module from Fig. 6 in a schematic cross-sectional view;

[0157] Fig. 9 shows a fourth embodiment of a perfusion module in a perspective exploded view and

[0158] Fig. 10 shows the perfusion module from Fig. 9 in a cross-sectional view.

[0159] Fig. 1 shows a first embodiment of a perfusion module 100 in a perspective exploded view. The perfusion module 100 comprises a base body 102, a flow guide element 104, a filter element 106, and a fastening element 108.

[0160] GO 241130WO December 17, 2025 The base body 102 has a fluid passage 110, a recess 112 converging towards the fluid passage 110, support elements 114, 116, a first connecting section 118 for connection to the fastening element 108, and a second connecting section 120 for connection to the wall area of ​​a fluid vessel of a bioreactor. The support elements 114, 116 are distributed over the surface of the recess 112 on the base body 102.

[0161] The flow guide element 104 is disc-shaped with an outer diameter 122 and has penetrating openings 124, 126, 128 as well as a plurality of spacers 130, 132. The flow guide element 104 is essentially rotationally symmetrical and the penetrating openings 124, 126, 128 have a decreasing size in the direction of the axis of rotation.

[0162] The filter element 106 is designed as a filter sheet made of a porous material. Furthermore, the filter element 106 is disc-shaped with an outer diameter of 134.

[0163] The fastening element 108 is designed as a fastening ring with an inner diameter 136 and with a connecting section 138, wherein the connecting section 138 of the fastening ring can be connected to the first connecting section 118 of the base body 102.

[0164] The outer diameter 122 of the flow guide element 104 and the outer diameter 134 of the filter element 106 are each larger than the inner diameter 136 of the fastening element 108. Thus, the perfusion module 100 is designed such that the fastening element 108 can serve to fasten the filter element 106 to the flow guide element 104, wherein, in an assembled state of the perfusion module 100, the flow guide element 104 is arranged between the base body 102 and the filter element 106.

[0165] GO 241130WO December 17, 2025 Fig. 2 shows the perfusion module 100 from Fig. 1 in a cross-sectional view. For this reason, the reference numerals used in the description of Fig. 1 to identify components of the perfusion module 100 are reused for the description of Fig. 2.

[0166] The perfusion module 100 is shown in an assembled state in Fig. 2. Here, the flow guide element 104 is arranged between the base body 102 and the filter element 106, with the fastening element 108 holding the filter element 106 and the flow guide element 104 to the base body 102. Thus, the recess 112 of the base body 102 and the flow guide element 104 together form a cavity 200 that is permeable to the fluid passage 110 and to the flow guide element 104. The flow guide element 104 is supported by the support elements 114, 116 of the base body 102.

[0167] The flow guide element 104 has a basic profile 202 with a curvature 204. The curvature 204 is directed towards the fluid passage 110 and the filter element 106 is locally bonded to the flow guide element 104, so that the filter element 106 assumes a curved position corresponding to the curvature 204 of the flow guide element 104.

[0168] Fig. 3 shows a first embodiment of a system 300 with a bioreactor 302 and with the perfusion module 100 from Fig. 1. Here too, the reference numerals used in the description of Fig. 1 to identify components of the perfusion module 100 are used for the description of Fig. 3 and supplemented as necessary.

[0169] The bioreactor 302 comprises a fluid vessel 304, a lid 306 compatible with the fluid vessel 304, and a gassing and stirring unit 308. The fluid vessel 304 has a wall section 310 and a bottom section 312.

[0170] GO 241130WO December 17, 2025, wherein the perfusion module 100 forms the bottom section 312 of the fluid vessel 304. The perfusion module 100 is connected to the wall section 310 via the second connecting section 120 of the base body 102. The perfusion module 100, or the filter element 106, the wall section 310, and the cover 306 together form a reactor chamber 314 in which the aeration and stirring unit 308 is arranged.

[0171] The gassing and stirring unit 308 has a membrane element 316 and is connected to the cover 306 via a connection 318, the connection 318 being provided both for fluid transfer, for example gas transfer, and for power transmission to drive the gassing and stirring unit 308.

[0172] The perfusion module 100 is connected to a pump 320 via the fluid passage 110.

[0173] Fig. 4 shows a second embodiment of a perfusion module 400 in a perspective exploded view. The perfusion module 400 comprises a base body 402, a flow guide element 404, a filter element 406, and a fastening element 408.

[0174] The base body 402 has a fluid passage 410, a recess 412 converging towards the fluid passage 410, support and segmentation elements 414, 416, and a connecting section 418 for connection to the fastening element 408. The support and segmentation elements 414, 416 are designed as elongated projections, each with a principal direction of extension 422, 424, and are arranged in the recess 412 such that the principal directions of extension 422, 424 converge. Furthermore, the support and segmentation elements 414, 416 have partially different lengths in order to direct and distribute the flow exiting or entering the fluid passage 410.

[0175] GO 241130WO December 17, 2025 The flow guiding element 404 is disc-shaped with an outer diameter 426 and has penetrating openings 428, 430 as well as a plurality of spacers 432, 434. The penetrating openings 428, 430 are distributed essentially homogeneously over the surface of the flow guiding element 404.

[0176] The filter element 406 is designed as a filter sheet made of a porous material. Furthermore, the filter element 406 is disc-shaped with an outer diameter of 436.

[0177] The perfusion module 400 is designed for a bioreactor with a fluid vessel having a wall section 438. The fastening element 408 is formed by an edge region of the wall section 438. The edge region, or fastening element 408, has a connecting section 440 for connection with the connecting section 418 of the base body 402 and an inner diameter 442. The outer diameter 426 of the flow guide element 404 and the outer diameter 436 of the filter element 406 are each larger than the inner diameter 442 of the fastening element 408. Thus, the perfusion module 400 is designed such that the fastening element 408 serves to fasten the filter element 406 to the flow guide element 404.

[0178] Fig. 5 shows the perfusion module 400 from Fig. 4 in a cross-sectional view. The perfusion module 400 is shown in an assembled state in Fig. 5.

[0179] The connecting section 440 of the fastening element 408 is connected to the connecting section 418 of the base body 402. This connects the filter element 406 and the flow guide element 404 to the base body 402, with the flow guide element 404 positioned between the base body 402 and the filter element 406. The flow guide element 404 and the recess 412 of the base body 402 thus form a cavity 500. The support and segmentation elements 414 and 416 are arranged within this cavity 500.

[0180] GO 241130WO December 17, 2025 The flow guide element 404 has a flat base profile 502. The filter element 406 is supported by the spacers 432, 434 of the flow guide element 404. Furthermore, the filter element 406 is welded to individual spacers 432, 434 in order to keep the filter element 406 in the position shown as far as possible when a pressure difference is applied between the reactor chamber and the cavity 500 of the perfusion module 400.

[0181] Fig. 6 shows a third embodiment of a perfusion module 600 in a perspective exploded view. The perfusion module 600 comprises a base body 602, a flow guide element 604, a filter element 606, and a fastening element 608.

[0182] The base body 602 has a fluid passage 610, a recess 612 converging towards the fluid passage 610, support and segmentation elements 614, 616 and a connecting section 618 for connection with the fastening element 608. The support and segmentation elements 614, 616 are designed as concentrically arranged projections.

[0183] The flow guidance element 604 is disc-shaped with an outer diameter 620 and has penetrating openings 622, 624 as well as a plurality of spacers 626, 628.

[0184] Filter element 606 is designed as a non-woven, porous filter medium. A specific example of such a filter medium is a filter fleece. Furthermore, filter element 606 is disc-shaped with an outer diameter.

[0185] The perfusion module 600 is designed for a bioreactor with a fluid vessel having a wall section 630. The fastening element 608 is formed by an edge region of the wall section 630. The edge region or fastening element 608 has a connecting section 632 for connection with

[0186] GO 241130WO 17 December 2025 the connecting section 618 of the base body 602 and an inner diameter 634.

[0187] The outer diameter 620 of the flow guide element 604 and the outer diameter of the filter element 606 are each larger than the inner diameter 634 of the fastening element 608. Thus, the perfusion module 600 is designed such that the fastening element 608 serves to fasten the filter element 606 to the flow guide element 604.

[0188] Fig. 7 shows the perfusion module 600 from Fig. 6 in a cross-sectional view. The perfusion module 600 is shown in an assembled state in Fig. 7.

[0189] The connecting section 632 of the fastening element 608 is connected to the connecting section 618 of the base body 602. This connects the filter element 606 and the flow guide element 604 to the base body 602, with the flow guide element 604 positioned between the base body 602 and the filter element 606. The flow guide element 604 and the recess 612 of the base body 602 thus form a cavity 700. The support and segmentation elements 614 and 616 are arranged in the cavity 700, having different heights to compensate for the slope of the recess 612. The filter element 606 is supported by the spacers 626 and 628 of the flow guide element 604.

[0190] The cross-sectional view shown shows that the flow-guiding element 604 has a base profile 702 with a camber 704, the camber 704 being directed in the opposite direction to the fluid outlet of the base body 602. It is further shown that the support and segmentation elements 614, 616 have different heights, the different heights of the support and segmentation elements 614, 616 being adapted to the camber 704 of the base profile 702 of the flow-guiding element 604.

[0191] GO 241130WO 17 December 2025. Furthermore, the spacers 626, 628 are essentially the same height relative to the basic profile 702 of the flow guidance element 604.

[0192] In the assembled state shown, the filter element 606 is clamped onto the spacers 626, 628 of the flow guide element 604 by the fastening element 608. In this state, the filter element 606 and the fastening element 608 together have a curved profile with a bulge 704 formed towards the center of the filter element 606, and the flanks of the bulge 704 form a medium collection channel 706 surrounding the filter element 606.

[0193] Fig. 8 shows a curved profile 800 of the perfusion module 600 from Fig. 6 in a schematic cross view.

[0194] The profile 800 of the bottom section has an edge section 802, a middle section 804, and a curvature 806 corresponding to a radius of curvature 808. Furthermore, the bottom section has a diameter da that corresponds to the inner diameter of the fluid container for which the bottom section is intended. The curvature 806 is designed such that the radius of curvature 808 has a value ranging from equal to five times the diameter da of the bottom section.

[0195] Viewed in the cross-sectional view shown, the curvature 806 forms a slope 812, 814 beyond the central region 804, which descends towards the edge region 802. The profile 800 rises in the edge region 802 in the opposite direction to the slope 812, 814 – upwards in this illustration – with a height h₁ or h₃. Thus, the curvature 806 and the edge region 802 together form a channel 816 surrounding the central region 804, which, when used in a bioreactor with such a bottom area, serves as a medium collection area.

[0196] GO 241130WO December 17, 2025 Fig. 9 shows a fourth embodiment of a perfusion module 900 in a perspective exploded view. The perfusion module 900 comprises a base body 902, a flow guide element 904, a filter element 906, and a fastening element 908.

[0197] The base body 902 has a fluid passage 910, a recess 912 converging towards the fluid passage 910, support and segmentation elements 914, 916, and a connecting section 918 for connection to the fastening element 908. The support and segmentation elements 914, 916 are designed as elongated projections, each with a principal direction of extension 920, 922, and are arranged in the recess 912 such that the principal directions of extension 920, 922 converge. Furthermore, the support and segmentation elements 914, 916 have partially different lengths in order to direct and distribute the flow exiting or entering the fluid passage 910.

[0198] The flow guidance element 904 is disc-shaped with an outer diameter 924 and has penetrating openings 926, 928 as well as a plurality of spacers 930, 932.

[0199] The filter element 906 is designed as a membrane fabric. Furthermore, the filter element 906 is disc-shaped with an outer diameter of 934.

[0200] The perfusion module 900 is designed for a bioreactor with a fluid vessel having a wall section 936. The fastening element 908 is formed by an edge region of the wall section 936. The edge region, or fastening element 908, has a connecting section 938 for connection to the connecting section 918 of the base body 902 and an inner diameter 940.

[0201] GO 241130WO December 17, 2025 The outer diameter 924 of the flow guide element 904 and the outer diameter 934 of the filter element 906 are each larger than the inner diameter 940 of the fastening element 908. Thus, the perfusion module 900 is designed such that the fastening element 908 serves to fasten the filter element 906 to the flow guide element 904.

[0202] Fig. 10 shows the perfusion module 900 from Fig. 9 in a cross-sectional view. The perfusion module 900 is shown in an assembled state in Fig. 10.

[0203] The connecting section 938 of the fastening element 908 is connected to the connecting section 918 of the base body 902. This connects the filter element 906 and the flow guide element 904 to the base body 902, with the flow guide element 904 positioned between the base body 902 and the filter element 906. The flow guide element 904 and the recess 912 of the base body 902 thus form a cavity 1000. The support and segmentation elements 914 and 916 are arranged in the cavity 1000. The filter element 906 is supported by the spacers 930 and 932 of the flow guide element 904.

[0204] The flow guide element 904 has a flat base profile 1002. The cross-sectional view shown shows that the spacers 930, 932 have different heights relative to the base profile 1002 of the flow guide element 904. The heights of the spacers 930, 932 are selected such that the filter element 906 is supported in a curved position. In the assembled state shown, the filter element 906 is clamped onto the spacers 930, 932 of the flow guide element 904 by the fastening element 908. In this state, the filter element 906 and the fastening element 908 together have a curved profile with a bulge 1004 formed towards the center of the filter element 906, and the flanks of the bulge 1004 form a medium collection channel 1006 surrounding the filter element 906.

[0205] GO 241130WO December 17, 2025 December 17, 2025

Claims

December 17, 2025 Patent claims 1. Perfusion module (100, 400, 600, 900) for a bioreactor, with a base body (102, 402, 602, 902) having a fluid passage (110, 410, 610, 910) and a recess (112, 412, 612, 912) converging towards the fluid passage (110, 410, 610, 910), with a flow-permeable flow guide element (104, 404, 604, 904), with a filter element (106, 406, 606, 906) and with at least one fastening element (108, 408, 608, 908), wherein the at least one fastening element (108, 408, 608, 908) is designed to fasten the filter element (106, 406, 606, 906) to the flow guide element (104, 404, 604, 904), wherein, in an assembled state of the perfusion module (100, 400, 600, 900), the flow guide element (104, 404, 604, 904) is arranged between the base body (102, 402, 602, 902) and the filter element (106, 406, 606, 906), and the recess (112, 412, 612, 912) of the base body (102, 402, 602, 902) and the flow guide element (104, 404, 604, 904) together form a cavity permeable to the fluid passage (110, 410, 610, 910) and to the flow guide element (104, 404, 604, 904). (200, 500, 700, 1000).

2. Perfusion module (100, 400, 600, 900) according to claim 1, characterized by that the filter element (106, 406, 606, 906) has a first separation efficiency, that the flow guidance element (104, 404, 604, 904) has a second separation efficiency, and that the first separation efficiency is greater than the second separation efficiency.

3. Perfusion module (100, 400, 600, 900) according to one of the preceding claims, characterized by that the flow guiding element (104, 404, 604, 904) has pores and / or penetrating openings (124, 126, 128, 428, 430, 622, 624, 926, 928) 4. Perfusion module (100, 400, 600, 900) according to claim 3, characterized by that the flow guidance element (104, 404, 604, 904) has a central area, wherein the central area is free of pores and / or free of penetrating openings.

5. Perfusion module (100, 400, 600, 900) according to claim 3 or 4, characterized by that the pores and / or the penetrating openings (124, 126, 128, 428, 430, 622, 624, 926, 928) are distributed essentially homogeneously over an area of ​​the flow guidance element (104, 404, 604, 904).

6. Perfusion module (100, 400, 600, 900) according to one of claims 3 to 5, characterized in that, that the pores and / or the penetrating openings (124, 126, 128, 428, 430, 622, 624, 926, 928) are distributed over an area of ​​the flow guidance element (104, 404, 604, 904) according to a mathematical function, the mathematical function being based on a trigonometric function based on a multiple of the golden angle and on a radial distribution function.

7. Perfusion module (100, 400, 600, 900) according to claim 3 or 4, characterized by GO 241130WO December 17, 2025 that the flow guidance element (104, 404, 604, 904) is essentially rotationally symmetrical relative to an axis of rotation and has a porosity that decreases in the direction of the axis of rotation, and / or that the flow guidance element (104, 404, 604, 904] is essentially rotationally symmetrical relative to an axis of rotation and the penetrating openings (124, 126, 128, 428, 430, 622, 624, 926, 928] have a size that decreases in the direction of the axis of rotation, and / or that the flow guidance element (104, 404, 604, 904) is essentially rotationally symmetrical relative to an axis of rotation and the penetrating openings (124, 126, 128, 428, 430, 622, 624, 926, 928) are arranged with a distance to each other that increases in the direction of the axis of rotation.

8. Perfusion module (100, 400, 600, 00) according to one of the preceding claims, characterized by that, viewed in a radial cross-sectional view, the flow guidance element (104, 404, 604, 904) has a basic profile (202, 502, 702, 1002), wherein the basic profile (202, 502, 702, 1002) is essentially flat, or that, viewed in a radial cross-sectional view, the flow guidance element (104, 404, 604, 904) has a basic profile (202, 502, 702, 1002), wherein the basic profile (202, 502, 702, 1002) is curved, or that, viewed in a radial cross-sectional view, the flow guidance element (104, 404, 604, 904) has a first basic profile (202, 502, 702, 1002) and a second basic profile (202, 502, 702, 1002), GO 241130WO December 17, 2025 wherein the first basic profile (202, 502, 702, 1002) is essentially flat and the second basic profile (202, 502, 702, 1002) is curved.

9. Perfusion module (100, 400, 600, 900) according to one of the preceding claims, characterized by that the flow guide element (104, 404, 604, 904) has at least one spacer (130, 132, 432, 434, 626, 628, 930, 932), and that the at least one spacer (130, 132, 432, 434, 626, 628, 930, 932) is designed to support the filter element (106, 406, 606, 906) held on the flow guide element (104, 404, 604, 904) by the at least one fastening element (108, 408, 608, 908).

10. Perfusion module (100, 400, 600, 900) according to claim 9, characterized by that at least two spacers (130, 132, 432, 434, 626, 628, 930, 932) are provided, wherein the at least two spacers (130, 132, 432, 434, 626, 628, 930, 932) are of essentially the same height relative to a basic profile (202, 502, 702, 1002) of the flow guidance element (104, 404, 604, 904), or that at least two spacers (130, 132, 432, 434, 626, 628, 930, 932) are provided, wherein the at least two spacers (130, 132, 432, 434, 626, 628, 930, 932) are of different heights relative to a basic profile (202, 502, 702, 1002) of the flow guidance element (104, 404, 604, 904).

11. Perfusion module (100, 400, 600, 900) according to claim 9 or 10, characterized by GO 241130WO December 17, 2025 that the at least one spacer (130, 132, 432, 434, 626, 628, 930, 932) or the at least two spacers (130, 132, 432, 434, 626, 628, 930, 932) form a rotationally symmetric pattern.

12. Perfusion module (100, 400, 600, 900) according to one of the preceding claims, characterized by that, in an assembled state of the perfusion module (100, 400, 600, 900), the filter element (106, 406, 606, 906) and the at least one fastening element (108, 408, 608, 908) together form a surface with a medium collection area (706, 1006).

13. Perfusion module (100, 400, 600, 900) according to claim 12, characterized by that, in an assembled state of the perfusion module (100, 400, 600, 900) and viewed in a radial cross-sectional view, the filter element (106, 406, 606, 906) and the at least one fastening element (108, 408, 608, 908) together have a curved profile with a curvature (204, 704, 806, 1004) formed towards the center of the filter element (106, 406, 606, 906), and that the flanks of the curvature (204, 704, 806, 1004) form a medium collection channel (706, 1006) surrounding the filter element (106, 406, 606, 906).

14. Perfusion module (100, 400, 600, 900) according to one of the preceding claims, characterized by that the recess (112, 412, 612, 912) of the base body (102, 402, 602, 902) has an outer perimeter and the fluid passage (110, 410, 610, 910) is substantially centered relative to the outer perimeter.

15. Perfusion module (100, 400, 600, 900) according to one of claims 1 to 13, GO 241130WO December 17, 2025 characterized by that the depression (112, 412, 612, 912) has an outer perimeter and the fluid passage (110, 410, 610, 910) is substantially decentered relative to the outer perimeter.

16. Perfusion module (100, 400, 600, 900) according to one of the preceding claims, characterized by that at least one support element (114, 116, 414, 416, 614, 616, 616, 914, 916) is provided in the recess (112, 412, 612, 912) of the base body (102, 402, 602, 902), and that the at least one support element (114, 116, 414, 416, 614, 616, 914, 916) is designed to support the flow guide element (104, 404, 604, 904) in an assembled state of the perfusion module (100, 400, 600, 900) and in the case of negative pressure or positive pressure in the cavity (200, 500, 700, 1000).

17. Perfusion module (100, 400, 600, 900) according to one of the preceding claims, characterized by that at least one segmentation element (414, 416, 614, 616, 916) is provided in the recess (112, 412, 612, 912) of the base body (102, 402, 602, 902) for segmentation, in particular for the essentially isovolumetric segmentation of a fluid flow over the volume of the cavity (200, 500, 700, 1000).

18. Perfusion module (100, 400, 600, 900) according to one of the preceding claims, characterized by that a bearing element is provided for receiving an axle. GO 241130WO December 17, 2025 19. Perfusion module (100, 400, 600, 900) according to claim 18, characterized by that the bearing element is formed on the fastening element, or that the bearing element is formed on the base body 20. Perfusion module (100, 400, 600, 900) according to claim 19, characterized by that the fastening element comprises a first fastening part and a second fastening part, wherein the first fastening part is designed for fastening the filter element to the flow guide element and / or to the base body, and wherein the second fastening part is designed for fastening the filter element to the flow guide element and / or to the base body and comprises the bearing element.

21. Perfusion module (100, 400, 600, 900) according to one of claims 18 to 20, characterized in that, that the recess of the base body has an outer circumference, and that, in an assembled state of the perfusion module, the bearing element is substantially centered relative to the outer circumference of the recess.

22. System (300) with a perfusion module (100, 400, 600, 900) according to one of the preceding claims and with a bioreactor comprising a fluid reservoir (304), characterized by that the perfusion module (100, 400, 600, 900) forms the bottom of the fluid container (304) 23. System (300) according to claim 22, GO 241130WO December 17, 2025 characterized by that the fluid container (304) comprises a wall with a bottom area (312) and a wall area (310, 438, 630, 936), that the wall area (310, 438, 630, 936) has at least one fastening element (108, 408, 608, 908) of the perfusion module (100, 400, 600, 900), and that the bottom area (312) is formed by the filter element (106, 406, 606, 906), the flow guide element (104, 404, 604, 904) and the base body (102, 402, 602, 902) of the perfusion module (100, 400, 600, 900).

24. System (300) according to claim 22, characterized by that the fluid container (304) comprises a one-piece wall with a bottom region (312) and a wall region (310, 438, 630, 936), wherein the bottom region (312) has the fluid passage (110, 410, 610, 910) and the recess (112, 412, 612, 912) and forms the base body (102, 402, 602, 902) of the perfusion module (100, 400, 600, 900), or that the fluid container (304) comprises a one-piece wall with a bottom region (312) and a wall region (310, 438, 630, 936), wherein the bottom region (312) forms the flow guidance element (104, 404, 604, 904) of the perfusion module (100, 400, 600, 900), or that the fluid vessel (304) comprises a one-piece wall with a bottom region (312) and a wall region (310, 438, 630, 936), wherein the bottom region (312) forms the filter element (106, 406, 606, 906) of the perfusion module (100, 400, 600, 900).

25. System according to any of the preceding claims, characterized by GO 241130WO December 17, 2025 that the bioreactor has a stirrer arranged in the fluid vessel with one axis of movement, and that the axis of movement is accommodated by the bearing element of the fastening element of the perfusion module. GO 241130WO December 17, 2025

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